BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004664
         (1488 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAH47641.1| progesterone 5beta reductase-B [Nicotiana tabacum]    329   e-176
gb|ADG41743.1| putative progesterone 5 beta-reductase [Nicotiana...   327   e-176
gb|AEX31545.1| putative steroid 5beta-reductase [Solanum lycoper...   333   e-174
dbj|BAH47640.1| progesterone 5beta reductase-A [Nicotiana tabacum]    330   e-174
gb|AEX31544.1| putative steroid 5beta-reductase [Solanum tuberosum]   332   e-173

>dbj|BAH47641.1| progesterone 5beta reductase-B [Nicotiana tabacum]
          Length = 388

 Score =  329 bits (844), Expect(2) = e-176
 Identities = 149/201 (74%), Positives = 170/201 (84%), Gaps = 2/201 (0%)
 Frame = -2

Query: 779 VEKKEGLTWSVHRPGNIFGFSPYSMMNLIGTLCVYATICKHEGVPMRFPGSKGIWEGYSD 600
           VEKKEGLTWSVHRPG IFGFSPYS+MN++GTLCVYA ICKHEG+P++FPG K  W+GYSD
Sbjct: 189 VEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVKAAWDGYSD 248

Query: 599 ASDADLIAEHQIWATVDPYAKNEAFNCSNGDVFKWKHLWKVFGEQFEVECVEGGE--KLC 426
            SDADLIAEHQIWA VDPYAKNEAFN  NGDVFKWKH WKV  EQF VE  E  E  +  
Sbjct: 249 CSDADLIAEHQIWAAVDPYAKNEAFNVRNGDVFKWKHFWKVLAEQFGVEAAEFDEVNRCT 308

Query: 425 LVELMKDKGPVWDEIVKKNGLTPTKLEEVAIWWFVDMVLGIESHPLDTMNKSKEHGFLGF 246
           L E+MKDKGPVWDEIVK+NGLTP KLE+V +WWFVD++L  +  PLDTMNKSKEHGFLGF
Sbjct: 309 LAEMMKDKGPVWDEIVKENGLTPAKLEDVGVWWFVDLILAGDC-PLDTMNKSKEHGFLGF 367

Query: 245 RNSKSSLITWIDRMKGYKIVP 183
           RNS+ + I+WID++K YK+VP
Sbjct: 368 RNSQKAFISWIDKVKAYKVVP 388



 Score =  316 bits (810), Expect(2) = e-176
 Identities = 139/167 (83%), Positives = 157/167 (94%)
 Frame = -3

Query: 1390 MSWWWSGAIGAARKRFEEDDAPPKYESVALVVGVTGIVGNSLAEILPLSDTPGGPWKVYG 1211
            MSWWW+GAIGAA+K+FEEDDAPPKY+SVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1    MSWWWAGAIGAAKKKFEEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 1210 VARRPRPSWNADHPVDYIQCDVSDPEDTQSKLSKLTDVTHIFYVTWSSRPTEAENCEING 1031
            VARRPRPSWNADHP+DYIQCD+S+PEDTQSKLS LTDVTH+FYVTW+SR TE ENCEING
Sbjct: 61   VARRPRPSWNADHPIDYIQCDISNPEDTQSKLSLLTDVTHVFYVTWASRSTEVENCEING 120

Query: 1030 KMFRNVLNALIPNATKLKHICLQTGRKHYIGPFELYGKVGHDPPYSE 890
            KMF+NVL+ +IPN   L+HICLQTGRKHY+GPFELYGKV HD P+ E
Sbjct: 121  KMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDFPFHE 167


>gb|ADG41743.1| putative progesterone 5 beta-reductase [Nicotiana tabacum]
          Length = 388

 Score =  327 bits (839), Expect(2) = e-176
 Identities = 149/201 (74%), Positives = 170/201 (84%), Gaps = 2/201 (0%)
 Frame = -2

Query: 779 VEKKEGLTWSVHRPGNIFGFSPYSMMNLIGTLCVYATICKHEGVPMRFPGSKGIWEGYSD 600
           VEKKEGLTWSVHRPG IFGFSPYS+MN++GTLCVYA ICKHEG+P++FPG K  W+GYSD
Sbjct: 189 VEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVKAAWDGYSD 248

Query: 599 ASDADLIAEHQIWATVDPYAKNEAFNCSNGDVFKWKHLWKVFGEQFEVECVEGGE--KLC 426
            SDADLIAEHQIWA VDPYAKNEAFN SNGDVFKWKH WKV  EQF VE  E  E  +  
Sbjct: 249 CSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVEAAEFDEVNRCT 308

Query: 425 LVELMKDKGPVWDEIVKKNGLTPTKLEEVAIWWFVDMVLGIESHPLDTMNKSKEHGFLGF 246
           L E+MKDKGPVWDEIVK+NGLT  KLE+V +WWFVD++L  +  PLDTMNKSKEHGFLGF
Sbjct: 309 LAEMMKDKGPVWDEIVKENGLTLAKLEDVGVWWFVDLILAGDC-PLDTMNKSKEHGFLGF 367

Query: 245 RNSKSSLITWIDRMKGYKIVP 183
           RNS+ + I+WID++K YK+VP
Sbjct: 368 RNSQKAFISWIDKVKAYKVVP 388



 Score =  317 bits (813), Expect(2) = e-176
 Identities = 139/167 (83%), Positives = 157/167 (94%)
 Frame = -3

Query: 1390 MSWWWSGAIGAARKRFEEDDAPPKYESVALVVGVTGIVGNSLAEILPLSDTPGGPWKVYG 1211
            MSWWW+GAIGAA+K+FEEDDAPPKY+SVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1    MSWWWAGAIGAAKKKFEEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 1210 VARRPRPSWNADHPVDYIQCDVSDPEDTQSKLSKLTDVTHIFYVTWSSRPTEAENCEING 1031
            VARRPRPSWNADHP+DYIQCD+S+PEDTQSKLS LTDVTH+FYVTW+SR TE ENCEING
Sbjct: 61   VARRPRPSWNADHPIDYIQCDISNPEDTQSKLSLLTDVTHVFYVTWASRSTEVENCEING 120

Query: 1030 KMFRNVLNALIPNATKLKHICLQTGRKHYIGPFELYGKVGHDPPYSE 890
            KMF+NVL+ +IPN   L+HICLQTGRKHY+GPFELYGKV HD P+ E
Sbjct: 121  KMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDSPFHE 167


>gb|AEX31545.1| putative steroid 5beta-reductase [Solanum lycopersicum]
          Length = 387

 Score =  333 bits (853), Expect(2) = e-174
 Identities = 151/201 (75%), Positives = 173/201 (86%), Gaps = 2/201 (0%)
 Frame = -2

Query: 779 VEKKEGLTWSVHRPGNIFGFSPYSMMNLIGTLCVYATICKHEGVPMRFPGSKGIWEGYSD 600
           VEKKEGLTWSVHRPG IFGFSPYS+MN++GTLCVYA ICKHEG+P++FPG KG W+GYSD
Sbjct: 188 VEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVKGAWDGYSD 247

Query: 599 ASDADLIAEHQIWATVDPYAKNEAFNCSNGDVFKWKHLWKVFGEQFEVECVE--GGEKLC 426
            SDADLIAEHQIWA VDPYAKNEAFN SNGDVFKWKH WKV GEQF +E  E   G++  
Sbjct: 248 CSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLGEQFGLEAAEFDEGKRCT 307

Query: 425 LVELMKDKGPVWDEIVKKNGLTPTKLEEVAIWWFVDMVLGIESHPLDTMNKSKEHGFLGF 246
           LVE+MKDKG VWDEIVK+NGL PTKLEEV +WWFVD++L  +   LDTMNKSKEHGFLGF
Sbjct: 308 LVEMMKDKGAVWDEIVKENGLVPTKLEEVGVWWFVDLILSGDC-ALDTMNKSKEHGFLGF 366

Query: 245 RNSKSSLITWIDRMKGYKIVP 183
           RNS+ + I+WID++K YK+VP
Sbjct: 367 RNSQKAFISWIDKVKAYKVVP 387



 Score =  308 bits (790), Expect(2) = e-174
 Identities = 134/164 (81%), Positives = 154/164 (93%)
 Frame = -3

Query: 1381 WWSGAIGAARKRFEEDDAPPKYESVALVVGVTGIVGNSLAEILPLSDTPGGPWKVYGVAR 1202
            WW+GAIGAA+K+FE+D+APPKYESVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYGVAR
Sbjct: 3    WWAGAIGAAKKKFEDDEAPPKYESVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGVAR 62

Query: 1201 RPRPSWNADHPVDYIQCDVSDPEDTQSKLSKLTDVTHIFYVTWSSRPTEAENCEINGKMF 1022
            R RPSWNADHP++Y+QCD+S+PEDTQSKLS LTDVTH+FYVTW++R TE ENCEINGKMF
Sbjct: 63   RARPSWNADHPIEYVQCDISNPEDTQSKLSVLTDVTHVFYVTWANRSTEVENCEINGKMF 122

Query: 1021 RNVLNALIPNATKLKHICLQTGRKHYIGPFELYGKVGHDPPYSE 890
            RNVLN +IPN   L+HICLQTGRKHY+GPFELYGKV HDPP+ E
Sbjct: 123  RNVLNVIIPNCPNLRHICLQTGRKHYLGPFELYGKVSHDPPFHE 166


>dbj|BAH47640.1| progesterone 5beta reductase-A [Nicotiana tabacum]
          Length = 389

 Score =  330 bits (846), Expect(2) = e-174
 Identities = 151/201 (75%), Positives = 172/201 (85%), Gaps = 2/201 (0%)
 Frame = -2

Query: 779 VEKKEGLTWSVHRPGNIFGFSPYSMMNLIGTLCVYATICKHEGVPMRFPGSKGIWEGYSD 600
           VEKKEGLTWSVHRPG IFGFSPYS+MN++GTLCVYA ICKHEG+P++FPG K  W+GYSD
Sbjct: 190 VEKKEGLTWSVHRPGVIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVKAAWDGYSD 249

Query: 599 ASDADLIAEHQIWATVDPYAKNEAFNCSNGDVFKWKHLWKVFGEQFEVECVEGGE--KLC 426
            SDADLIAEHQIWA VDPYAKNEAFN SNGDVFKWKH WKV  EQF VE  E  E  +  
Sbjct: 250 CSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVEAAEFDEENRCT 309

Query: 425 LVELMKDKGPVWDEIVKKNGLTPTKLEEVAIWWFVDMVLGIESHPLDTMNKSKEHGFLGF 246
           LVE+MKDKGPVWDEIVK+NGLTPTKLE+V +WWFVD++L  +   LDTMNKSKEHGFLGF
Sbjct: 310 LVEMMKDKGPVWDEIVKENGLTPTKLEDVGVWWFVDLMLAGDCR-LDTMNKSKEHGFLGF 368

Query: 245 RNSKSSLITWIDRMKGYKIVP 183
           RNS+ + I+WID++K YK+VP
Sbjct: 369 RNSQKAFISWIDKVKAYKVVP 389



 Score =  310 bits (794), Expect(2) = e-174
 Identities = 137/168 (81%), Positives = 157/168 (93%), Gaps = 1/168 (0%)
 Frame = -3

Query: 1390 MSWWWSGAIGAARKRFEEDDAPPKYESVALVVGVTGIVGNSLAEILPLSDTPGGPWKVYG 1211
            MSWWW+GAIGAA+K+FEEDDAPPKY+SVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1    MSWWWAGAIGAAKKKFEEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 1210 VARRPRPSWNADHPVDYIQCDVSDPEDTQSKLSKLTDVTHIFYVTWSSRPTEAENCEING 1031
            +ARRPRPSWNADHP+DYIQCD+S+PEDTQSKLS LTDVTH+FYVTW+SR TE ENCEING
Sbjct: 61   LARRPRPSWNADHPIDYIQCDISNPEDTQSKLSLLTDVTHVFYVTWASRSTEVENCEING 120

Query: 1030 KMFRNVLNALIPNATKLKHICLQTGRKHYIGPFELYGK-VGHDPPYSE 890
            KMF+NV++ +IPN   L+HICLQTGRKHY+GPFELYGK V HD P+ E
Sbjct: 121  KMFQNVVSVVIPNCPNLRHICLQTGRKHYLGPFELYGKLVAHDSPFHE 168


>gb|AEX31544.1| putative steroid 5beta-reductase [Solanum tuberosum]
          Length = 387

 Score =  332 bits (852), Expect(2) = e-173
 Identities = 151/201 (75%), Positives = 172/201 (85%), Gaps = 2/201 (0%)
 Frame = -2

Query: 779 VEKKEGLTWSVHRPGNIFGFSPYSMMNLIGTLCVYATICKHEGVPMRFPGSKGIWEGYSD 600
           VEKKEGLTWSVHRPG IFGFSPYS+MN++GTLCVYA ICKHEG+P++FPG KG W+GYSD
Sbjct: 188 VEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVKGAWDGYSD 247

Query: 599 ASDADLIAEHQIWATVDPYAKNEAFNCSNGDVFKWKHLWKVFGEQFEVECVE--GGEKLC 426
            SDADLIAEHQIWA VDPYAKNEAFN SNGDVFKWKH WKV  EQF VE  E   G++  
Sbjct: 248 CSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVEAAEFDEGKRCT 307

Query: 425 LVELMKDKGPVWDEIVKKNGLTPTKLEEVAIWWFVDMVLGIESHPLDTMNKSKEHGFLGF 246
           LVE+MKDKG VWDEIVK+NGL PTKLEEV +WWF D++L  +  PLDTMNKSKEHGFLGF
Sbjct: 308 LVEMMKDKGAVWDEIVKENGLVPTKLEEVGVWWFADLMLSGDC-PLDTMNKSKEHGFLGF 366

Query: 245 RNSKSSLITWIDRMKGYKIVP 183
           RNS+ + I+WID++K YK+VP
Sbjct: 367 RNSQKAFISWIDKVKAYKVVP 387



 Score =  304 bits (778), Expect(2) = e-173
 Identities = 133/164 (81%), Positives = 152/164 (92%)
 Frame = -3

Query: 1381 WWSGAIGAARKRFEEDDAPPKYESVALVVGVTGIVGNSLAEILPLSDTPGGPWKVYGVAR 1202
            WW+GAIGAA+K+FEED+APPKYESVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYGVAR
Sbjct: 3    WWAGAIGAAKKKFEEDEAPPKYESVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGVAR 62

Query: 1201 RPRPSWNADHPVDYIQCDVSDPEDTQSKLSKLTDVTHIFYVTWSSRPTEAENCEINGKMF 1022
            R RPSWNADHP++Y+QCD+S+PEDTQSKLS LTDVTH+FYVTW++R  E ENCEINGKMF
Sbjct: 63   RARPSWNADHPIEYVQCDISNPEDTQSKLSVLTDVTHVFYVTWANRSKEVENCEINGKMF 122

Query: 1021 RNVLNALIPNATKLKHICLQTGRKHYIGPFELYGKVGHDPPYSE 890
            RNVLN +IPN   L+HICLQTGRKHY+GPFELYGKV HD P+ E
Sbjct: 123  RNVLNVIIPNCPHLRHICLQTGRKHYLGPFELYGKVSHDSPFHE 166