BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004558
         (1437 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADE06393.1| stearoyl-acyl-carrier protein desaturase [Macadam...   607   e-171
gb|AFS68798.1| stearoyl-acyl desaturase [Solanum cardiophyllum]       604   e-170
gb|AFS68797.1| stearoyl-acyl desaturase [Solanum acaule]              604   e-170
sp|Q41319.2|STAD_SOLCO RecName: Full=Acyl-[acyl-carrier-protein]...   602   e-170
ref|XP_002274708.2| PREDICTED: acyl-[acyl-carrier-protein] desat...   602   e-170

>gb|ADE06393.1| stearoyl-acyl-carrier protein desaturase [Macadamia tetraphylla]
          Length = 396

 Score =  607 bits (1564), Expect = e-171
 Identities = 304/397 (76%), Positives = 332/397 (83%), Gaps = 3/397 (0%)
 Frame = +3

Query: 132  MALKLNHSITFQPHKVSSFGLSQSTHLRSHKVSMASTLRVPSMEVEQVKKPFTPPREVHV 311
            MALKLN  + FQ  K  SFGL  + +LRS KV MASTLR  S EVE +K+PF+PPREVHV
Sbjct: 1    MALKLN-PLLFQSQKYPSFGLPTAANLRSPKVFMASTLRSSSKEVENLKRPFSPPREVHV 59

Query: 312  QVTHTMPPEKVDIFKSLHSWAEKDLLVHLKPVEKCWQPNDFLPDPASEGFMEQIKELRER 491
            QVTH+MPP+K++IFKSL  WAEK++L+HLK VEK WQP DFLPDPASEGF EQ+KELRER
Sbjct: 60   QVTHSMPPQKIEIFKSLDDWAEKNILMHLKSVEKSWQPQDFLPDPASEGFDEQVKELRER 119

Query: 492  SMEIPDEYY---VXXXXXXXXXXXXXXXXXXXXXXXDETGASLTPWAVWTRAWTAEENRH 662
            + EIPD+Y+   V                       DETGASLT WAVWTRAWTAEENRH
Sbjct: 120  AKEIPDDYFIVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAVWTRAWTAEENRH 179

Query: 663  GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 842
            GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTEN+PYLGFIYTSFQERATFISHGNT
Sbjct: 180  GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNT 239

Query: 843  ARHAKEYGDLKLAQVCGIIAADEKRHETAYTKIVEKLLEIDPNDTVLALADMMKKKISMP 1022
            AR AKE+GD+KLAQ+CG IAADEKRHETAYTKIVEKL EIDP+ TVLA ADMM+KKISMP
Sbjct: 240  ARLAKEHGDMKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMP 299

Query: 1023 AHLMYDGQDDNLFEHFSSVAQRLGVYTAKDYADILEFLVERWNIEKLTGLSCEGNKAQDF 1202
            AHLMYDGQDDNLFEHFS+VAQRLGVYTA+DYADILEFLV RWN+EKLTGLS EG KAQDF
Sbjct: 300  AHLMYDGQDDNLFEHFSAVAQRLGVYTARDYADILEFLVGRWNVEKLTGLSGEGQKAQDF 359

Query: 1203 VCGLAPRIRRLEERAQARAKKKSTAPFSWIFGKEIQL 1313
            VCGLAPRIRRLEERAQ R K+ S  PFSWIF KE+ L
Sbjct: 360  VCGLAPRIRRLEERAQGRTKQASKIPFSWIFDKEVML 396


>gb|AFS68798.1| stearoyl-acyl desaturase [Solanum cardiophyllum]
          Length = 393

 Score =  604 bits (1558), Expect = e-170
 Identities = 302/397 (76%), Positives = 335/397 (84%), Gaps = 3/397 (0%)
 Frame = +3

Query: 132  MALKLNHSITFQPHKVSSFGLSQSTHLRSHKVSMASTLRVPSMEVEQVKKPFTPPREVHV 311
            MAL  N S TFQ  K +      S+ LRS +V MASTLR PS+E   VKKPF+PPREVHV
Sbjct: 1    MALNFN-SATFQSIKTTR---RPSSSLRSPRVFMASTLRPPSVEGGNVKKPFSPPREVHV 56

Query: 312  QVTHTMPPEKVDIFKSLHSWAEKDLLVHLKPVEKCWQPNDFLPDPASEGFMEQIKELRER 491
            QVTH+MPPEK +IF SLH WAE ++LVHLKPVE+CWQ +DFLPDPASEGF +QIKELRER
Sbjct: 57   QVTHSMPPEKREIFDSLHGWAENNILVHLKPVEECWQASDFLPDPASEGFEDQIKELRER 116

Query: 492  SMEIPDEYYVXXXXXXXXXXXXXXXXXXXXXXX---DETGASLTPWAVWTRAWTAEENRH 662
              EIPD+Y+V                          DETG SLTPWA+WTRAWTAEENRH
Sbjct: 117  CKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGVSLTPWAIWTRAWTAEENRH 176

Query: 663  GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 842
            GDLLNKYLYLSGRVDM+QIEKTIQYL+GSGMDPRTENNPYLGFIYTSFQERATFISHGNT
Sbjct: 177  GDLLNKYLYLSGRVDMRQIEKTIQYLVGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 236

Query: 843  ARHAKEYGDLKLAQVCGIIAADEKRHETAYTKIVEKLLEIDPNDTVLALADMMKKKISMP 1022
            ARHAKE+GD+KLAQVCGIIAADEKRHETAYTKIVEKL E+DP+ TVLA+ADMM+KKISMP
Sbjct: 237  ARHAKEHGDMKLAQVCGIIAADEKRHETAYTKIVEKLFEVDPDGTVLAVADMMRKKISMP 296

Query: 1023 AHLMYDGQDDNLFEHFSSVAQRLGVYTAKDYADILEFLVERWNIEKLTGLSCEGNKAQDF 1202
            AHLMYDG+DDNLFEHFS+VAQRLGVYTAKDYADILEFLV RW IEKLTGLS EG+KAQD+
Sbjct: 297  AHLMYDGRDDNLFEHFSAVAQRLGVYTAKDYADILEFLVGRWEIEKLTGLSGEGHKAQDY 356

Query: 1203 VCGLAPRIRRLEERAQARAKKKSTAPFSWIFGKEIQL 1313
            VCGLAPRIR+LEERAQARAK+K+  PFSW+FGK+I+L
Sbjct: 357  VCGLAPRIRKLEERAQARAKQKAPVPFSWVFGKDIKL 393


>gb|AFS68797.1| stearoyl-acyl desaturase [Solanum acaule]
          Length = 393

 Score =  604 bits (1558), Expect = e-170
 Identities = 304/397 (76%), Positives = 334/397 (84%), Gaps = 3/397 (0%)
 Frame = +3

Query: 132  MALKLNHSITFQPHKVSSFGLSQSTHLRSHKVSMASTLRVPSMEVEQVKKPFTPPREVHV 311
            MAL  N S TFQ  K +    S    LRS +V MASTLR PS+E   VKKPF+PPREVHV
Sbjct: 1    MALNFN-SPTFQSIKTTRRPCSP---LRSPRVFMASTLRPPSVEGGNVKKPFSPPREVHV 56

Query: 312  QVTHTMPPEKVDIFKSLHSWAEKDLLVHLKPVEKCWQPNDFLPDPASEGFMEQIKELRER 491
            QVTH+MPPEK +IF SLH WAE ++LVHLKPVEKCWQ +DFLPDPASEGF +Q+KELRER
Sbjct: 57   QVTHSMPPEKREIFDSLHGWAENNILVHLKPVEKCWQASDFLPDPASEGFEDQVKELRER 116

Query: 492  SMEIPDEYYVXXXXXXXXXXXXXXXXXXXXXXX---DETGASLTPWAVWTRAWTAEENRH 662
              EIPD+Y+V                          DETGASLTPWA+WTRAWTAEENRH
Sbjct: 117  CKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTPWAIWTRAWTAEENRH 176

Query: 663  GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 842
            GDLLNKYLYLSGRVDM+QIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT
Sbjct: 177  GDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 236

Query: 843  ARHAKEYGDLKLAQVCGIIAADEKRHETAYTKIVEKLLEIDPNDTVLALADMMKKKISMP 1022
            ARHAKE+GD+KLAQVCGIIAADEKRHE AYTKIVEKL E+DP+ TVLA+ADMM+KKISMP
Sbjct: 237  ARHAKEHGDMKLAQVCGIIAADEKRHEAAYTKIVEKLFEVDPDGTVLAVADMMRKKISMP 296

Query: 1023 AHLMYDGQDDNLFEHFSSVAQRLGVYTAKDYADILEFLVERWNIEKLTGLSCEGNKAQDF 1202
            AHLMYDGQDDNLFEHFS+VAQRLGVYTAKDYADILEFLV RW IEKLTGLS EG+KAQD+
Sbjct: 297  AHLMYDGQDDNLFEHFSAVAQRLGVYTAKDYADILEFLVGRWEIEKLTGLSGEGHKAQDY 356

Query: 1203 VCGLAPRIRRLEERAQARAKKKSTAPFSWIFGKEIQL 1313
            VCGLAPRIR+LEERAQARAK+K+  PFSW+FGK+I+L
Sbjct: 357  VCGLAPRIRKLEERAQARAKQKAPVPFSWVFGKDIKL 393


>sp|Q41319.2|STAD_SOLCO RecName: Full=Acyl-[acyl-carrier-protein] desaturase, chloroplastic;
            AltName: Full=Stearoyl-ACP desaturase; Flags: Precursor
            gi|85857257|emb|CAA55535.2| stearoyl-acyl carrier protein
            desaturase [Solanum commersonii]
          Length = 393

 Score =  602 bits (1552), Expect = e-170
 Identities = 302/397 (76%), Positives = 335/397 (84%), Gaps = 3/397 (0%)
 Frame = +3

Query: 132  MALKLNHSITFQPHKVSSFGLSQSTHLRSHKVSMASTLRVPSMEVEQVKKPFTPPREVHV 311
            MAL  N S TFQ  K +    S    LRS +V MASTLR PS+E   VKKPF+PPREVHV
Sbjct: 1    MALNFN-SPTFQSIKTTRRPCSP---LRSPRVFMASTLRPPSVEDGNVKKPFSPPREVHV 56

Query: 312  QVTHTMPPEKVDIFKSLHSWAEKDLLVHLKPVEKCWQPNDFLPDPASEGFMEQIKELRER 491
            QVTH+MPPEK +IF SLH WA+ ++LVHLKPVEKCWQ +DFLPDPASEGF +Q+KELRER
Sbjct: 57   QVTHSMPPEKREIFDSLHGWADNNILVHLKPVEKCWQASDFLPDPASEGFEDQVKELRER 116

Query: 492  SMEIPDEYYVXXXXXXXXXXXXXXXXXXXXXXX---DETGASLTPWAVWTRAWTAEENRH 662
              EIPD+Y+V                          DETGASLTPWA+WTRAWTAEENRH
Sbjct: 117  CKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTPWAIWTRAWTAEENRH 176

Query: 663  GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 842
            GDLLNKYLYLSGRVDM+QIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT
Sbjct: 177  GDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 236

Query: 843  ARHAKEYGDLKLAQVCGIIAADEKRHETAYTKIVEKLLEIDPNDTVLALADMMKKKISMP 1022
            ARHAKE+GD+KLAQVCGIIAADEKRHETAYTKIVEKL E+DP+ TVLA+ADMM+KKISMP
Sbjct: 237  ARHAKEHGDMKLAQVCGIIAADEKRHETAYTKIVEKLFEVDPDGTVLAVADMMRKKISMP 296

Query: 1023 AHLMYDGQDDNLFEHFSSVAQRLGVYTAKDYADILEFLVERWNIEKLTGLSCEGNKAQDF 1202
            AHLMYDG+DDNLFEHFS+VAQRLGVYTAKDYADILEFLV RW IEKLTGLS EG+KA+D+
Sbjct: 297  AHLMYDGRDDNLFEHFSTVAQRLGVYTAKDYADILEFLVGRWEIEKLTGLSGEGHKARDY 356

Query: 1203 VCGLAPRIRRLEERAQARAKKKSTAPFSWIFGKEIQL 1313
            VCGLAPRIR+LEERAQARAK+K+  PFSW+FGK+I+L
Sbjct: 357  VCGLAPRIRKLEERAQARAKQKAPVPFSWVFGKDIKL 393


>ref|XP_002274708.2| PREDICTED: acyl-[acyl-carrier-protein] desaturase, chloroplastic-like
            [Vitis vinifera]
          Length = 396

 Score =  602 bits (1552), Expect = e-170
 Identities = 294/397 (74%), Positives = 335/397 (84%), Gaps = 3/397 (0%)
 Frame = +3

Query: 132  MALKLNHSITFQPHKVSSFGLSQSTHLRSHKVSMASTLRVPSMEVEQVKKPFTPPREVHV 311
            MAL+LNH ++FQ HK  SF L     +RS  V MASTLR  S EVE +KKPF+PPREVHV
Sbjct: 1    MALQLNH-LSFQFHKFPSFSLPPMASIRSPTVFMASTLRSGSKEVENLKKPFSPPREVHV 59

Query: 312  QVTHTMPPEKVDIFKSLHSWAEKDLLVHLKPVEKCWQPNDFLPDPASEGFMEQIKELRER 491
            QVTH+MPP+K++IFKS+ +WAE+++LVHLKPVEK WQP DFLPDPAS+GF EQ+KELRER
Sbjct: 60   QVTHSMPPQKIEIFKSMENWAEQNILVHLKPVEKSWQPQDFLPDPASDGFHEQVKELRER 119

Query: 492  SMEIPDEYYVXXXXXXXXXXXXXXXXXXXXXXX---DETGASLTPWAVWTRAWTAEENRH 662
            + E+PD+Y+V                          DETGASLT WA+WTRAWTAEENRH
Sbjct: 120  AKELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAIWTRAWTAEENRH 179

Query: 663  GDLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNT 842
            GDLLNKYLYLSGRVDM+QIEKTIQYLIGSGMDPRTEN+PYLGFIYTSFQERATFISHGNT
Sbjct: 180  GDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNT 239

Query: 843  ARHAKEYGDLKLAQVCGIIAADEKRHETAYTKIVEKLLEIDPNDTVLALADMMKKKISMP 1022
            ARHAKE+GD+KLAQ+CG IA+DEKRHETAYTKIVEKL EIDP+ TVLA ADMM+KKISMP
Sbjct: 240  ARHAKEHGDMKLAQICGTIASDEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMP 299

Query: 1023 AHLMYDGQDDNLFEHFSSVAQRLGVYTAKDYADILEFLVERWNIEKLTGLSCEGNKAQDF 1202
            AHLMYDG+DDNLF+HFS+VAQRLGVYTAKDYADILEFLV RWN+EKLTGLS +G KAQD+
Sbjct: 300  AHLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVGRWNVEKLTGLSGDGRKAQDY 359

Query: 1203 VCGLAPRIRRLEERAQARAKKKSTAPFSWIFGKEIQL 1313
            VCGLAPR R+LEERAQ RAK+  T PFSWIF +E++L
Sbjct: 360  VCGLAPRFRKLEERAQGRAKEALTIPFSWIFDREVKL 396