BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004545
         (1403 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270537.2| PREDICTED: E3 ubiquitin-protein ligase UBR2-...   379   e-102
ref|XP_002532325.1| ubiquitin ligase E3 alpha, putative [Ricinus...   375   e-101
emb|CBI30553.3| unnamed protein product [Vitis vinifera]              359   1e-96
ref|XP_003530206.1| PREDICTED: E3 ubiquitin-protein ligase UBR3-...   356   7e-96
ref|XP_003623126.1| E3 ubiquitin-protein ligase ubr1 [Medicago t...   352   1e-94

>ref|XP_002270537.2| PREDICTED: E3 ubiquitin-protein ligase UBR2-like [Vitis vinifera]
          Length = 2048

 Score =  379 bits (972), Expect = e-102
 Identities = 216/469 (46%), Positives = 287/469 (61%), Gaps = 2/469 (0%)
 Frame = -3

Query: 1401 TLDNKREGAALPFESLEEQMYAMIRGALHDKLLHLDSANTIEDTAAREDISTRNEEAKTL 1222
            T ++  E  +  F++LEE MY  I+  + + L H +   T E  +A E    R   A  +
Sbjct: 1313 TSNDTGERTSYNFDTLEEDMYLCIQKEMCNLLTHSNLV-TDEKFSAAEGGPKRGVNAGEV 1371

Query: 1221 LLGKYIAAXXXXXXXXXXXXXXXXXXXXXXXSKENTAPHAYDGFGPAGCDGIYLSSCGHA 1042
            LLGKYIA                        S+  T   AYDG GP+ CDGI+LSSCGHA
Sbjct: 1372 LLGKYIATLSRAAKENPSASGNAQSHNDRAMSESTTLVPAYDGLGPSDCDGIHLSSCGHA 1431

Query: 1041 VHEGCLNQYLFSLKDRYTRRIDFEGGDFMDPDQVEFLCPVCRGLANSVLPALPADSEKLC 862
            VH+GCL++YL SLK+R         G        EFLCPVCR LANSVLPALP DS+K  
Sbjct: 1432 VHQGCLDRYLSSLKER--GHYGLSNG--------EFLCPVCRQLANSVLPALPGDSQKGW 1481

Query: 861  RHPMAPTACPSDTLVAFTPCDVDLHPXXXXXXXXXXXXXADISVRDEIIKAVPLQHDGKK 682
            +     +A   D   + T  + +++               ++  + EI+K +P++  G+ 
Sbjct: 1482 KKLTISSAGSPDAAGSLTTLNDEINSLCIQQALSLLQSACNVVGKGEILKTIPMEGIGRI 1541

Query: 681  WPNPESALRVLCGMYFPMK-NKISGSGRVSYSMMMWDTLKYSIITTEIAARSCRTTLSPE 505
             P  E  LR++C MYFP K +K+SGS RVS  ++MWD LKYS+I+TEIA+R  RT+ +P 
Sbjct: 1542 APTIEPFLRMICRMYFPGKYDKVSGSTRVSQFIIMWDILKYSLISTEIASRCGRTSTTPT 1601

Query: 504  FSLNALQKELKSSSGFALSLLLKNVQYSRTNDSLSVLLRLRGIKLFAESICSGFSVENFP 325
            + +++L KEL SS+GF L+LLL  VQ  R  +   VLLR RGI+LFA S+C G SV+ FP
Sbjct: 1602 YCVDSLYKELNSSTGFILTLLLSIVQSMRNENPHHVLLRFRGIQLFAGSVCHGISVDEFP 1661

Query: 324  SHMCRQGA-MLHILENAETEVRYPDTQFWTRASDPILAHDAFSSLMWILFCLPWPTLVCK 148
            S    QG  ML ILE+ ETEV YPD QFW RASDP+LAHD FSSL+W+LFCLP+P L+CK
Sbjct: 1662 STASTQGGNMLSILEHIETEVSYPDIQFWKRASDPVLAHDPFSSLIWVLFCLPYPFLLCK 1721

Query: 147  ESFLSLVHIFYAVTITQAIITYGGKRQHNITEMGYHDGLISDIYKFMGE 1
            E F SLVH++YAV++ QAIITY GK+Q  I  +G+ D LI+DI   +G+
Sbjct: 1722 EVFFSLVHLYYAVSVVQAIITYCGKQQCKINGLGFQDCLITDISNIVGK 1770


>ref|XP_002532325.1| ubiquitin ligase E3 alpha, putative [Ricinus communis]
            gi|223527968|gb|EEF30052.1| ubiquitin ligase E3 alpha,
            putative [Ricinus communis]
          Length = 2073

 Score =  375 bits (962), Expect = e-101
 Identities = 212/460 (46%), Positives = 281/460 (61%), Gaps = 2/460 (0%)
 Frame = -3

Query: 1374 ALPFESLEEQMYAMIRGALHDKLLHLDSANTIEDTAAREDISTRNEEAKTLLLGKYIAAX 1195
            A   E+LE   Y  IR  +++  +   S     D +A E     N    ++LLGKYIAA 
Sbjct: 1328 ACSLETLERDYYISIRKEINNHTIFSSSGLKDVDISAGEGGLKSNRGVSSVLLGKYIAAF 1387

Query: 1194 XXXXXXXXXXXXXXXXXXXXXXSKENTAPHAYDGFGPAGCDGIYLSSCGHAVHEGCLNQY 1015
                                   K  +   AY+ FGPA CDG+YLSSCGHAVH+GCL++Y
Sbjct: 1388 SREITEHPSSSENSLDDIA----KRESTLQAYEKFGPADCDGVYLSSCGHAVHQGCLDRY 1443

Query: 1014 LFSLKDRYTRRIDFEGGDFMDPDQVEFLCPVCRGLANSVLPALPADSEKLCRHPMAPTAC 835
            L SLK+R+ RR+ FEGG  +DPDQ EFLCPVCR L+NS+LP+LP D +++ + PM  T  
Sbjct: 1444 LSSLKERFVRRLVFEGGHIVDPDQGEFLCPVCRRLSNSILPSLPGDFQRVWKEPMISTVS 1503

Query: 834  PSDTLV-AFTPCDVDLHPXXXXXXXXXXXXXADISVRDEIIKAVPLQHDGKKWPNPESAL 658
             +D +   F  C+                  A++  + +I K  PLQ + +   + +S  
Sbjct: 1504 STDAVGHLFASCEGS-DSLWLPRALSLLQSAANMIQKGDIWKTFPLQRNERMKQDLDSIS 1562

Query: 657  RVLCGMYFPMK-NKISGSGRVSYSMMMWDTLKYSIITTEIAARSCRTTLSPEFSLNALQK 481
            RVL  MYFP + +K S S R +  M+MWDTLKYS+++ EIAARS R  ++P +SL+AL K
Sbjct: 1563 RVLFKMYFPSRQDKFSRSTRANQFMIMWDTLKYSLVSMEIAARSGRIHMTPTYSLDALYK 1622

Query: 480  ELKSSSGFALSLLLKNVQYSRTNDSLSVLLRLRGIKLFAESICSGFSVENFPSHMCRQGA 301
            EL+SSSGF L+LLLK V   R+ +SL VL R RGI+LFA+SICSG S ++      R+G 
Sbjct: 1623 ELQSSSGFVLALLLKIVHSLRSKNSLHVLQRFRGIQLFAKSICSGVSADHASRTCGRKGD 1682

Query: 300  MLHILENAETEVRYPDTQFWTRASDPILAHDAFSSLMWILFCLPWPTLVCKESFLSLVHI 121
               IL+  E E+ YPD QFW +A+DPIL HDAFSSLMW+LFCLP P L C+ES LSLVHI
Sbjct: 1683 ASSILKQVEKELPYPDIQFWNQAADPILIHDAFSSLMWVLFCLPHPFLSCEESLLSLVHI 1742

Query: 120  FYAVTITQAIITYGGKRQHNITEMGYHDGLISDIYKFMGE 1
            FY V+I QAI+   G  Q+N  + G+HD LI+DI   + E
Sbjct: 1743 FYLVSIAQAILAIYGPDQYNNRKPGFHDCLITDISHVLEE 1782


>emb|CBI30553.3| unnamed protein product [Vitis vinifera]
          Length = 1254

 Score =  359 bits (921), Expect = 1e-96
 Identities = 192/379 (50%), Positives = 253/379 (66%), Gaps = 4/379 (1%)
 Frame = -3

Query: 1125 KENTAPHAYDGFGPAGCDGIYLSSCGHAVHEGCLNQYLFSLKDRYTRRIDFEGGDFMDPD 946
            +  T   AYDG GP+ CDGI+LSSCGHAVH+GCL++YL SLK+R      F     M  +
Sbjct: 601  ESTTLVPAYDGLGPSDCDGIHLSSCGHAVHQGCLDRYLSSLKERLQ---PFALNFLMATE 657

Query: 945  QVE--FLCPVCRGLANSVLPALPADSEKLCRHPMAPTACPSDTLVAFTPCDVDLHPXXXX 772
              E  FLCPVCR LANSVLPALP DS+K  +     +A   D   + T  + +++     
Sbjct: 658  STEGEFLCPVCRQLANSVLPALPGDSQKGWKKLTISSAGSPDAAGSLTTLNDEINSLCIQ 717

Query: 771  XXXXXXXXXADISVRDEIIKAVPLQHDGKKWPNPESALRVLCGMYFPMK-NKISGSGRVS 595
                      ++  + EI+K +P++  G+  P  E  LR++C MYFP K +K+SGS RVS
Sbjct: 718  QALSLLQSACNVVGKGEILKTIPMEGIGRIAPTIEPFLRMICRMYFPGKYDKVSGSTRVS 777

Query: 594  YSMMMWDTLKYSIITTEIAARSCRTTLSPEFSLNALQKELKSSSGFALSLLLKNVQYSRT 415
              ++MWD LKYS+I+TEIA+R  RT+ +P + +++L KEL SS+GF L+LLL  VQ  R 
Sbjct: 778  QFIIMWDILKYSLISTEIASRCGRTSTTPTYCVDSLYKELNSSTGFILTLLLSIVQSMRN 837

Query: 414  NDSLSVLLRLRGIKLFAESICSGFSVENFPSHMCRQGA-MLHILENAETEVRYPDTQFWT 238
             +   VLLR RGI+LFA S+C G SV+ FPS    QG  ML ILE+ ETEV YPD QFW 
Sbjct: 838  ENPHHVLLRFRGIQLFAGSVCHGISVDEFPSTASTQGGNMLSILEHIETEVSYPDIQFWK 897

Query: 237  RASDPILAHDAFSSLMWILFCLPWPTLVCKESFLSLVHIFYAVTITQAIITYGGKRQHNI 58
            RASDP+LAHD FSSL+W+LFCLP+P L+CKE F SLVH++YAV++ QAIITY GK+Q  I
Sbjct: 898  RASDPVLAHDPFSSLIWVLFCLPYPFLLCKEVFFSLVHLYYAVSVVQAIITYCGKQQCKI 957

Query: 57   TEMGYHDGLISDIYKFMGE 1
              +G+ D LI+DI   +G+
Sbjct: 958  NGLGFQDCLITDISNIVGK 976


>ref|XP_003530206.1| PREDICTED: E3 ubiquitin-protein ligase UBR3-like [Glycine max]
          Length = 2037

 Score =  356 bits (914), Expect = 7e-96
 Identities = 206/470 (43%), Positives = 276/470 (58%), Gaps = 3/470 (0%)
 Frame = -3

Query: 1401 TLDNKREGAALPFESLEEQMYAMIRGALHDKLLHLDSANTIEDTAAREDISTRNEEAKTL 1222
            T  + +E     FE+LE+ MY  +R  +HD LL  +  N  E  +     S    +  ++
Sbjct: 1293 TYYHDKENTPYTFETLEQGMYFSVRDEMHDLLLSSNLLNEDEKVSTVGGNSNFIIDTGSV 1352

Query: 1221 LLGKYIAAXXXXXXXXXXXXXXXXXXXXXXXSKENTAPH-AYDGFGPAGCDGIYLSSCGH 1045
            LLGKY A                          E+T+ H AYDGFGP  CDG++LSSCGH
Sbjct: 1353 LLGKYTADLVQEMSEVSSVSENASNETASV---ESTSQHPAYDGFGPTDCDGVHLSSCGH 1409

Query: 1044 AVHEGCLNQYLFSLKDRYTRRIDFEGGDFMDPDQVEFLCPVCRGLANSVLPALPADSEKL 865
            AVH+GCL++YL SLK+R  RRI FEGG  +DPDQ EFLCPVCR LAN VLP LP + +K 
Sbjct: 1410 AVHQGCLDRYLSSLKERSVRRIVFEGGHIVDPDQGEFLCPVCRRLANCVLPTLPGELQKP 1469

Query: 864  CRHPMAPTACPSDTLVAFTPCDVDLHPXXXXXXXXXXXXXADISVRDEIIKAVPLQHDGK 685
             +     +    +T           +              A+   +D+ + A+PL H  +
Sbjct: 1470 FKQSTILSTSSINTAPPLAELSELTYSLRLHLGLKLLQSAANAVGKDKFLNAIPLHHIDR 1529

Query: 684  KWPNPESALRVLCGMYFPMKN-KISGSGRVSYSMMMWDTLKYSIITTEIAARSCRTTLSP 508
               N E  +  L  MY P K  K+S   R+++SM+MWDTLKYS+ + EIAAR  +T+ +P
Sbjct: 1530 TRTNLEKFIWGLSKMYSPCKEEKLSRFSRLNHSMLMWDTLKYSLTSMEIAARCGKTSFTP 1589

Query: 507  EFSLNALQKELKSSSGFALSLLLKNVQYSRTNDSLSVLLRLRGIKLFAESICSGFSVENF 328
             F+L+AL +ELKSSSGF LSL+LK VQ +R+N+SL VL R RG++L AESICSG S+   
Sbjct: 1590 NFALSALYEELKSSSGFILSLMLKLVQKTRSNNSLHVLQRFRGVQLLAESICSGVSLNYA 1649

Query: 327  PSHMCRQGAMLHILENAETEVRYPDTQFWTRASDPILAHDAFSSLMWILFCLPWPTLVCK 148
             +    +G ML IL+  E ++   +  FW++ASDP+L HD FS+LMW+LFCLP P L C+
Sbjct: 1650 NNDESGRGDMLSILKQIEMDLSNTNISFWSQASDPVLLHDPFSTLMWVLFCLPHPFLSCE 1709

Query: 147  ESFLSLVHIFYAVTITQAIITYGGKRQHNIT-EMGYHDGLISDIYKFMGE 1
            ES LSLVH+FY V +TQAII Y  K +   + E    D LI+DIY  M E
Sbjct: 1710 ESLLSLVHVFYIVAVTQAIILYYEKSKDKPSRESALSDCLITDIYNVMDE 1759


>ref|XP_003623126.1| E3 ubiquitin-protein ligase ubr1 [Medicago truncatula]
            gi|355498141|gb|AES79344.1| E3 ubiquitin-protein ligase
            ubr1 [Medicago truncatula]
          Length = 2105

 Score =  352 bits (903), Expect = 1e-94
 Identities = 205/472 (43%), Positives = 281/472 (59%), Gaps = 8/472 (1%)
 Frame = -3

Query: 1392 NKREGAALPFESLEEQMYAMIRGALHDKLLHLDSANTIEDTAAREDISTRNEEAKT---- 1225
            +++E +   F++LE+ M+  IR  +HD    L S+NT+ +    E +ST    +      
Sbjct: 1280 DEKEKSPYTFDTLEQVMHVSIRDEMHD----LSSSNTMNED---EKVSTAEGNSNVRITE 1332

Query: 1224 -LLLGKYIAAXXXXXXXXXXXXXXXXXXXXXXXSKENTAPH-AYDGFGPAGCDGIYLSSC 1051
              LLGKY A                          E+T+PH + DGFGP  CDG++LSSC
Sbjct: 1333 CALLGKYAADVVKEMSEISSASGNASNENASV---ESTSPHLSNDGFGPTDCDGVHLSSC 1389

Query: 1050 GHAVHEGCLNQYLFSLKDRYTRRIDFEGGDFMDPDQVEFLCPVCRGLANSVLPALPADSE 871
            GHAVH+GCLN+YL SLK+R  RRI FEGG  +DPDQ E LCPVCR L N VLP LP +  
Sbjct: 1390 GHAVHQGCLNRYLSSLKERSVRRIVFEGGHIVDPDQGEILCPVCRRLVNGVLPTLPGELH 1449

Query: 870  KLCRHPMAPTACPSDTLVAFTPCDVDLHPXXXXXXXXXXXXXADISVRDEIIKAVPLQHD 691
                 P+  +A    +   F   +   +              A+   +D+ +KA+PL H 
Sbjct: 1450 T----PLVLSASSIHSTSPFADSNGATYSLRIQEALNLLKSAANAVGKDQFLKAIPLHHI 1505

Query: 690  GKKWPNPESALRVLCGMYFPMK-NKISGSGRVSYSMMMWDTLKYSIITTEIAARSCRTTL 514
             +  PN E     L  MYFP K +K+S   +V++S++MWDTLKYS+ + EI AR  +T+L
Sbjct: 1506 DETRPNVEKFSLGLSKMYFPGKQDKLSRFSKVNHSLLMWDTLKYSLTSMEIVARCGKTSL 1565

Query: 513  SPEFSLNALQKELKSSSGFALSLLLKNVQYSRTNDSLSVLLRLRGIKLFAESICSGFSVE 334
            +P F+L+A+ KEL+SSSGF L +LLK VQ +R+ +S+ VL R RG++LFAESICSG S+ 
Sbjct: 1566 TPNFALSAMYKELESSSGFILYMLLKLVQKTRSKNSIHVLQRFRGVQLFAESICSGVSLS 1625

Query: 333  NFPSHMCRQGAMLHILENAETEVRYPDTQFWTRASDPILAHDAFSSLMWILFCLPWPTLV 154
            +  + +  +G ML +L++ E +    D  FW  ASDP+LAHD FS+LMW+LFCLP P L 
Sbjct: 1626 HADNVISGRGDMLSVLKHIEMDQSNTDICFWNEASDPVLAHDPFSTLMWVLFCLPHPFLS 1685

Query: 153  CKESFLSLVHIFYAVTITQAIITYGGKR-QHNITEMGYHDGLISDIYKFMGE 1
            C+ES LSLVH FY V +TQAII Y  K    + +E    D +I+DI K MGE
Sbjct: 1686 CEESLLSLVHAFYMVAVTQAIILYHEKSLDKSSSESTLSDCMITDINKIMGE 1737