BLASTX nr result
ID: Bupleurum21_contig00004542
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004542
(1800 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003538733.1| PREDICTED: oxysterol-binding protein-related... 707 0.0
ref|XP_004163646.1| PREDICTED: oxysterol-binding protein-related... 679 0.0
ref|XP_004150860.1| PREDICTED: LOW QUALITY PROTEIN: oxysterol-bi... 674 0.0
ref|XP_002529779.1| Oxysterol-binding protein, putative [Ricinus... 625 e-177
gb|ABR16670.1| unknown [Picea sitchensis] 618 e-174
>ref|XP_003538733.1| PREDICTED: oxysterol-binding protein-related protein 2A-like [Glycine
max]
Length = 739
Score = 707 bits (1825), Expect = 0.0
Identities = 375/603 (62%), Positives = 432/603 (71%), Gaps = 14/603 (2%)
Frame = +2
Query: 2 FVLRHGVLSYSKIHRLENLALLSPGNXXXXXXXXXXXXXX--------TRPKNGET---- 145
F+LR+GVLSY+KI ENL LL P + TR K+ +T
Sbjct: 73 FLLRNGVLSYAKIRCPENLNLLIPSDDVRLIGEISTNRLARVDGAATVTRRKHRKTSSSS 132
Query: 146 GIVQLKISSFRESRSDNRRFYIFTATKTLHLRTHSRKERLAWIRALVXXXXXXXXXXXXX 325
G+V LKISSFRES+SD+RRFYIFTATKT HLRT SRK+R+AWI+ALV
Sbjct: 133 GVVHLKISSFRESKSDDRRFYIFTATKTFHLRTDSRKDRVAWIQALVSTRGLYPLQPLND 192
Query: 326 XXXXXTPSNISLSTAKLKKRLRENGVSEMLVNDCEQIMISEFSDIQGQVEVLCEERSNLL 505
P+NISLST +LK RL E G E LV +CEQI+++EFS++QGQ+EVLC+ERSNLL
Sbjct: 193 HLLL-APNNISLSTERLKIRLLEEGTGENLVKECEQILLTEFSEMQGQLEVLCQERSNLL 251
Query: 506 GTLWQLEAANFEAKTSGATDCE*YHTRKLEYSSSSLGRGKYTEYSTTESPDDAGKQELEE 685
T+ QLEAAN E + + + E Y K E+ SSLGRGKY+E STTES DD KQE+EE
Sbjct: 252 DTIRQLEAANTEPEAAAIHNGE-YQLTKNEF--SSLGRGKYSECSTTESSDDIEKQEMEE 308
Query: 686 VSXXXXXXXXXXXXXXXXAITSCRSVGVGHMADSVDGQINDADRINSNRDISVCGYPPFE 865
VS C S G + +P
Sbjct: 309 VSDEDEISYYDTRECFTEPDCKCESAGAMNQ------------------------HPQIA 344
Query: 866 RRNKLPDPVEKEKGVSLWSMIKDLVGKDLTRVCLPVYFNEPISTLQKCFEELEYSHLLDR 1045
RR KLPDPVEKEKGVSLWSMIKD +GKDLTRVCLPVYFNEPIS+LQKC E+LEYS+LLD+
Sbjct: 345 RRKKLPDPVEKEKGVSLWSMIKDNLGKDLTRVCLPVYFNEPISSLQKCCEDLEYSYLLDQ 404
Query: 1046 AYEYGKEGNSLLRILNVAAFAVSGYASTDGRHCKPFNPMLGETYEADYPEKGVRFFSEKV 1225
AYEYGK GN LLR L VAAFAVSGYAS++GR+CKPFNP+LGETYEADYP+KG+RFFSEKV
Sbjct: 405 AYEYGKSGNGLLRALYVAAFAVSGYASSEGRNCKPFNPLLGETYEADYPDKGIRFFSEKV 464
Query: 1226 SHHPTLIACHCEGKGWKFWGDSNLRSKFWGTSIQIDPLGTLTLEFDDGEIFQWSKVTTTI 1405
SHHPTLIACHCEG+GWKFW D+N+ SKFWG SIQ+DP+G LTLEF DGEIFQWSKVTTTI
Sbjct: 465 SHHPTLIACHCEGRGWKFWADTNIHSKFWGRSIQLDPVGVLTLEFSDGEIFQWSKVTTTI 524
Query: 1406 YNLILGKLYCDHHGTMQICGNRQYSCTLKFKEQSILDRSPRQVHGYVEDDTGEKVATLVG 1585
YNLILGK+YCDHHG M I GNR YSC LKFKEQ+ILDR+PRQVHG++ED TG+KVATL G
Sbjct: 525 YNLILGKIYCDHHGNMDIRGNRTYSCRLKFKEQTILDRNPRQVHGFIEDVTGKKVATLFG 584
Query: 1586 KWDEHMYYTIGDRVSKPKDV--SSASLLWKRNETPPNLTRYNLTPFAITLNELTPGLQLC 1759
KWD+ MYY G+ KPKD S+ASLLWKR PNLTRYNLTPFAITLNELTPGL+
Sbjct: 585 KWDDSMYYMNGNVNVKPKDFTSSNASLLWKRTMPSPNLTRYNLTPFAITLNELTPGLKEK 644
Query: 1760 LIP 1768
L P
Sbjct: 645 LPP 647
>ref|XP_004163646.1| PREDICTED: oxysterol-binding protein-related protein 2A-like [Cucumis
sativus]
Length = 726
Score = 679 bits (1751), Expect = 0.0
Identities = 355/593 (59%), Positives = 426/593 (71%), Gaps = 4/593 (0%)
Frame = +2
Query: 2 FVLRHGVLSYSKIHRLENLALLSPGNXXXXXXXXXXXXXXTRPKNGETG-IVQLKISSFR 178
FVLR GVLSYSKI +L+SP + +R K +TG IV LK+S FR
Sbjct: 80 FVLRDGVLSYSKI------SLISPDDDDVRLIGEISTNRLSRRKRHKTGGIVHLKVSRFR 133
Query: 179 ESRSDNRRFYIFTATKTLHLRTHSRKERLAWIRALVXXXXXXXXXXXXXXXXXXTPSNIS 358
ES+SD+RRFYIFTATKTLHLRT S+K+R+ WI+AL P+++S
Sbjct: 134 ESKSDDRRFYIFTATKTLHLRTESKKDRVTWIQALASSRSLFPLQIVNDNLPF-VPNDLS 192
Query: 359 LSTAKLKKRLRENGVSEMLVNDCEQIMISEFSDIQGQVEVLCEERSNLLGTLWQLEAANF 538
LST KLKKRL E G+S+ +V DCEQI++SEFS++QGQ++VL EERS L+ TL +LEAAN
Sbjct: 193 LSTQKLKKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEERSCLIDTLRELEAANI 252
Query: 539 EAKTSGATDCE*YHTRKLEYSSSSLGRGKYTEYSTTESPDDAGKQELEEVSXXXXXXXXX 718
E + +G + E Y K ++S+ GKY+E +TTES DD KQ+LE+ S
Sbjct: 253 EIEANGVHNGE-YQLMKHDFSTEC--SGKYSECTTTESSDDVEKQDLEDGSDADESFFYD 309
Query: 719 XXXXXXXAITSCRSVGVGHMADSVDGQINDADRINSNRDISVCGYPPFERRNKLPDPVEK 898
SC S RI ++ SV +RR KLPDPVEK
Sbjct: 310 TKETFSELSLSCGS------------------RIENSVHFSV------QRRKKLPDPVEK 345
Query: 899 EKGVSLWSMIKDLVGKDLTRVCLPVYFNEPISTLQKCFEELEYSHLLDRAYEYGKEGNSL 1078
EK VSLWSMIKD VGKDLTRVCLPVYFNEPIS+LQKC E++EYSHLLD+AYEYG++GN+
Sbjct: 346 EKRVSLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEYGRQGNNF 405
Query: 1079 LRILNVAAFAVSGYASTDGRHCKPFNPMLGETYEADYPEKGVRFFSEKVSHHPTLIACHC 1258
LR+L VAAFAVSGYAS++GRHCKPFNP+LGETYEAD+PEKG+RFFSEKVSHHPT+IACHC
Sbjct: 406 LRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHC 465
Query: 1259 EGKGWKFWGDSNLRSKFWGTSIQIDPLGTLTLEFDDGEIFQWSKVTTTIYNLILGKLYCD 1438
EG+GW+FWGDSNLRSKFWG SIQ+DP+G L LEFDDGE+FQWSKVTT IYNLILGK+YCD
Sbjct: 466 EGRGWRFWGDSNLRSKFWGRSIQLDPVGVLNLEFDDGEVFQWSKVTTNIYNLILGKVYCD 525
Query: 1439 HHGTMQICGNRQYSCTLKFKEQSILDRSPRQVHGYVEDDTGEKVATLVGKWDEHMYYTIG 1618
HHGTM I GNR+YSCTLKFKE SILDR+P QVHG++ED +G KVAT+ GKWDE MYY G
Sbjct: 526 HHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFIEDASGRKVATIFGKWDEGMYYVEG 585
Query: 1619 DRVSKPK---DVSSASLLWKRNETPPNLTRYNLTPFAITLNELTPGLQLCLIP 1768
++ PK S +LWK + PP+LTRYNLT FAITLNELTP L+ L P
Sbjct: 586 EKNWNPKRNRPPSDELMLWKSEKPPPDLTRYNLTSFAITLNELTPDLKERLPP 638
>ref|XP_004150860.1| PREDICTED: LOW QUALITY PROTEIN: oxysterol-binding protein-related
protein 2A-like [Cucumis sativus]
Length = 728
Score = 674 bits (1738), Expect = 0.0
Identities = 355/595 (59%), Positives = 426/595 (71%), Gaps = 6/595 (1%)
Frame = +2
Query: 2 FVLRHGVLSYSKIHRLENLALLSPGNXXXXXXXXXXXXXXTRPKNGETG-IVQLKISSFR 178
FVLR GVLSYSKI +L+SP + +R K +TG IV LK+S FR
Sbjct: 80 FVLRDGVLSYSKI------SLISPDDDDVRLIGEISTNRLSRRKRHKTGGIVHLKVSRFR 133
Query: 179 ESRSDNRRFY--IFTATKTLHLRTHSRKERLAWIRALVXXXXXXXXXXXXXXXXXXTPSN 352
ES+SD+RRFY IFTATKTLHLRT S+K+R+ WI+AL P++
Sbjct: 134 ESKSDDRRFYYSIFTATKTLHLRTESKKDRVTWIQALASSRSLFPLQIVNDNLPF-VPND 192
Query: 353 ISLSTAKLKKRLRENGVSEMLVNDCEQIMISEFSDIQGQVEVLCEERSNLLGTLWQLEAA 532
+SLST KLKKRL E G+S+ +V DCEQI++SEFS++QGQ++VL EERS L+ TL +LEAA
Sbjct: 193 LSLSTQKLKKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEERSCLIDTLRELEAA 252
Query: 533 NFEAKTSGATDCE*YHTRKLEYSSSSLGRGKYTEYSTTESPDDAGKQELEEVSXXXXXXX 712
N E + +G + E Y K ++S+ GKY+E +TTES DD KQ+LE+ S
Sbjct: 253 NIEIEANGVHNGE-YQLMKHDFSTEC--SGKYSECTTTESSDDVEKQDLEDGSDADESFF 309
Query: 713 XXXXXXXXXAITSCRSVGVGHMADSVDGQINDADRINSNRDISVCGYPPFERRNKLPDPV 892
SC S RI ++ SV +RR KLPDPV
Sbjct: 310 YDTKETFSELSLSCGS------------------RIENSVHFSV------QRRKKLPDPV 345
Query: 893 EKEKGVSLWSMIKDLVGKDLTRVCLPVYFNEPISTLQKCFEELEYSHLLDRAYEYGKEGN 1072
EKEK VSLWSMIKD VGKDLTRVCLPVYFNEPIS+LQKC E++EYSHLLD+AYEYG++GN
Sbjct: 346 EKEKRVSLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEYGRQGN 405
Query: 1073 SLLRILNVAAFAVSGYASTDGRHCKPFNPMLGETYEADYPEKGVRFFSEKVSHHPTLIAC 1252
+ LR+L VAAFAVSGYAS++GRHCKPFNP+LGETYEAD+PEKG+RFFSEKVSHHPT+IAC
Sbjct: 406 NFLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIAC 465
Query: 1253 HCEGKGWKFWGDSNLRSKFWGTSIQIDPLGTLTLEFDDGEIFQWSKVTTTIYNLILGKLY 1432
HCEG+GW+FWGDSNLRSKFWG SIQ+DP+G L LEFDDGE+FQWSKVTT IYNLILGK+Y
Sbjct: 466 HCEGRGWRFWGDSNLRSKFWGRSIQLDPVGVLNLEFDDGEVFQWSKVTTNIYNLILGKVY 525
Query: 1433 CDHHGTMQICGNRQYSCTLKFKEQSILDRSPRQVHGYVEDDTGEKVATLVGKWDEHMYYT 1612
CDHHGTM I GNR+YSCTLKFKE SILDR+P QVHG++ED +G KVAT+ GKWDE MYY
Sbjct: 526 CDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFIEDASGRKVATIFGKWDEGMYYV 585
Query: 1613 IGDRVSKPK---DVSSASLLWKRNETPPNLTRYNLTPFAITLNELTPGLQLCLIP 1768
G++ PK S +LWK + PP+LTRYNLT FAITLNELTP L+ L P
Sbjct: 586 EGEKNWNPKRNRPPSDELMLWKSEKPPPDLTRYNLTSFAITLNELTPDLKERLPP 640
>ref|XP_002529779.1| Oxysterol-binding protein, putative [Ricinus communis]
gi|223530723|gb|EEF32593.1| Oxysterol-binding protein,
putative [Ricinus communis]
Length = 563
Score = 625 bits (1613), Expect = e-177
Identities = 316/450 (70%), Positives = 359/450 (79%), Gaps = 6/450 (1%)
Frame = +2
Query: 437 MISEFSDIQGQVEVLCEERSNLLGTLWQLEAANFEAKTSGATDCE*YHTRKLEYSSSSLG 616
M+SEFS+IQGQ+++LCEERS+LL TL QLEAAN EA+T+G D E + K E+SS LG
Sbjct: 1 MLSEFSEIQGQLKILCEERSSLLDTLRQLEAANIEAETTGIPDGE-FQLTKHEFSS--LG 57
Query: 617 RGKYTEYSTTESPDDAGKQELEEVSXXXXXXXXXXXXXXXXAIT---SCRSVGVGHMADS 787
RGK++E STTES DD KQELEEVS S + V
Sbjct: 58 RGKFSECSTTESSDDIEKQELEEVSEEDESSFYDTKEYFTEPTFVRGSIKEVACHQEKHR 117
Query: 788 VDGQINDADRINSNRDISVCGYPPFERRNKLPDPVEKEKGVSLWSMIKDLVGKDLTRVCL 967
Q ++ +++++ + + GYP ERR KLPDPVEKEKGVSLWSMIKD VGKDL+RVCL
Sbjct: 118 EHFQSDNEEKMHARKQLCKSGYPHVERRKKLPDPVEKEKGVSLWSMIKDNVGKDLSRVCL 177
Query: 968 PVYFNEPISTLQKCFEELEYSHLLDRAYEYGKEGNSLLRILNVAAFAVSGYASTDGRHCK 1147
PVYFNEPIS+LQKCFE+LEYS+LLDRAYEYGKEGNSL+RILNVAAFAVSGYAS++GRHCK
Sbjct: 178 PVYFNEPISSLQKCFEDLEYSYLLDRAYEYGKEGNSLMRILNVAAFAVSGYASSEGRHCK 237
Query: 1148 PFNPMLGETYEADYPEKGVRFFSEKVSHHPTLIACHCEGKGWKFWGDSNLRSKFWGTSIQ 1327
PFNP+LGETYEAD+P+KGVRFFSEKVSHHPT+IACHCEG+GWKFWGDSNLRSKFWG SIQ
Sbjct: 238 PFNPLLGETYEADFPDKGVRFFSEKVSHHPTVIACHCEGRGWKFWGDSNLRSKFWGRSIQ 297
Query: 1328 IDPLGTLTLEFDDGEIFQWSKVTTTIYNLILGKLYCDHHGTMQICGNRQYSCTLKFKEQS 1507
+DP+G LTLEFDDGEIFQWSKVTTTIYNLILGKLYCDHHG M I GN QYSC LKFKEQS
Sbjct: 298 LDPVGVLTLEFDDGEIFQWSKVTTTIYNLILGKLYCDHHGPMHIQGNGQYSCKLKFKEQS 357
Query: 1508 ILDRSPRQVHGYVEDDTGEKVATLVGKWDEHMYYTIGDRVSKPKDVSS---ASLLWKRNE 1678
ILDR+P QV+G+VED G KVATL GKWD+ MYYT GD SK KD SS A+LLW+ +
Sbjct: 358 ILDRNPHQVNGFVEDTMGNKVATLFGKWDDSMYYTKGDGSSKTKDCSSSPNATLLWRSTK 417
Query: 1679 TPPNLTRYNLTPFAITLNELTPGLQLCLIP 1768
PPNLTRYNLT FAITLNELTPGLQ L P
Sbjct: 418 PPPNLTRYNLTSFAITLNELTPGLQEKLPP 447
>gb|ABR16670.1| unknown [Picea sitchensis]
Length = 785
Score = 618 bits (1593), Expect = e-174
Identities = 330/606 (54%), Positives = 403/606 (66%), Gaps = 17/606 (2%)
Frame = +2
Query: 2 FVLRHGVLSYSKIHRLENLALLSPGNXXXXXXXXXXXXXXTRPKNG---------ETGIV 154
FVL GVLSY KIH +++ +PK+ G V
Sbjct: 98 FVLHDGVLSYYKIHGPHKISVSRETEKAVRVIGEDSLKLIRKPKSQGIEPSHRRRPLGEV 157
Query: 155 QLKISSFRESRSDNRRFYIFTATKTLHLRTHSRKERLAWIRALVXXXXXXXXXXXXXXXX 334
LK+SS RESRSD++RF +FT TK LHLR SR++R AWI AL
Sbjct: 158 HLKVSSIRESRSDDKRFSVFTGTKRLHLRAESREDRAAWIEALQAAKDLFPHISINDLMS 217
Query: 335 XXTPSNISLSTAKLKKRLRENGVSEMLVNDCEQIMISEFSDIQGQVEVLCEERSNLLGTL 514
NI++ST KL+ RL E G+SE + + EQIM++EFS +Q Q+ +L +++ LL TL
Sbjct: 218 PI--ENITVSTEKLRCRLLEAGLSEAAIQESEQIMMAEFSVLQNQLNLLQQKQFRLLETL 275
Query: 515 WQLEAANFEAKTSGATDCE*YHTRKLEYSSSSLGRGKYTEYSTTESPDDAGKQELEEVSX 694
QLE F+ +T + + R SSS GKY+E S T+S DD +Q+ E
Sbjct: 276 RQLETEKFDLETKVVDESQ---HRSKHVSSSKSRNGKYSEGSVTDSDDDNERQDGPEEES 332
Query: 695 XXXXXXXXXXXXXXXAITSCRSVGVGHMADSVD---GQINDADRINSNRDISVCGY--PP 859
+ S R GH SVD G R + D+ + G+ P
Sbjct: 333 DEDESTYFDTREFLSS-NSLRRTDSGHRRSSVDSDEGLALSNSRFGVDVDMQMVGFDHPY 391
Query: 860 FERRNKLPDPVEKEKGVSLWSMIKDLVGKDLTRVCLPVYFNEPISTLQKCFEELEYSHLL 1039
+RR LP+P EKEKGVSLWSMIKD +GKDLT+VCLPVYFNEP+S+LQKCFE+LEYS+LL
Sbjct: 392 VQRRKNLPEPKEKEKGVSLWSMIKDNIGKDLTKVCLPVYFNEPLSSLQKCFEDLEYSYLL 451
Query: 1040 DRAYEYGKEGNSLLRILNVAAFAVSGYASTDGRHCKPFNPMLGETYEADYPEKGVRFFSE 1219
DRAYE+GK GN+L+RILNVAAFAVSGYASTDGR+CKPFNP+LGETYEADYP+KG+RF SE
Sbjct: 452 DRAYEWGKRGNNLMRILNVAAFAVSGYASTDGRNCKPFNPLLGETYEADYPDKGIRFISE 511
Query: 1220 KVSHHPTLIACHCEGKGWKFWGDSNLRSKFWGTSIQIDPLGTLTLEFDDGEIFQWSKVTT 1399
KVSHHP ++ACHCEG+GWKFWGDSNL+SKFWG SIQ+DP+G LTLEFDDGE+F WSKVTT
Sbjct: 512 KVSHHPMIVACHCEGRGWKFWGDSNLKSKFWGRSIQLDPIGVLTLEFDDGEVFHWSKVTT 571
Query: 1400 TIYNLILGKLYCDHHGTMQICGNRQYSCTLKFKEQSILDRSPRQVHGYVEDDTGEKVATL 1579
+IYNLILGKLYCDH+GTM+I GN +YSC LKFKEQSI+DR+P QV G V++ TG V+TL
Sbjct: 572 SIYNLILGKLYCDHYGTMRIQGNHEYSCKLKFKEQSIIDRNPHQVQGLVQNRTGNTVSTL 631
Query: 1580 VGKWDEHMYYTIGDRVSKPK---DVSSASLLWKRNETPPNLTRYNLTPFAITLNELTPGL 1750
GKWDE +YY D SK K S A LLW+RN+ P N TRYNLT FAITLNELTPGL
Sbjct: 632 FGKWDESLYYINDDAASKSKGDDPSSEAVLLWERNKPPKNPTRYNLTRFAITLNELTPGL 691
Query: 1751 QLCLIP 1768
+ L P
Sbjct: 692 KEKLPP 697