BLASTX nr result
ID: Bupleurum21_contig00004360
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004360
(1176 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002314534.1| predicted protein [Populus trichocarpa] gi|2... 108 2e-21
ref|XP_002314537.1| predicted protein [Populus trichocarpa] gi|2... 108 3e-21
ref|XP_002314535.1| predicted protein [Populus trichocarpa] gi|2... 107 6e-21
gb|AAS02080.1| protein disulfide isomerase [Quercus suber] 107 6e-21
emb|CAC87937.1| PDI-like protein [Quercus suber] 107 6e-21
>ref|XP_002314534.1| predicted protein [Populus trichocarpa] gi|222863574|gb|EEF00705.1|
predicted protein [Populus trichocarpa]
Length = 564
Score = 108 bits (271), Expect = 2e-21
Identities = 83/271 (30%), Positives = 121/271 (44%), Gaps = 7/271 (2%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK--NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPV 193
+ T +L+ Y+ LK+K NFEVVLI + D EE F ++F+TMPWLALPFKD
Sbjct: 214 EFTPKLVELYKTLKEKRENFEVVLISLDDE--------EEDFKESFETMPWLALPFKDKS 265
Query: 194 IKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDLE 373
+KL R P LV++G GK P A+++ GI+AYPFT + +L
Sbjct: 266 CEKLVRYFEL-------RTIPNLVIIGQDGKTLNPNVAELIEEHGIEAYPFTPEKLDELA 318
Query: 374 -VRKVK----EVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTF 538
+ K K ++S ++ N I S+V ++ GK ++ P FL
Sbjct: 319 AIEKAKLESQTLESVLVIGENDFVIDKSGSKVPVSELVGKNILLYFSAQWCPPCRAFLPK 378
Query: 539 LKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGSPLLLELLHDAFGD 718
L Y+ D FEVI + S +E +++PWL F +G +L G
Sbjct: 379 LIEAYHTIKRKDNAFEVIFISSDRDQSTFDEFYSEMPWLALPFGDGRKQILSRKFKIQGI 438
Query: 719 ARLVAFDLHGEVVRKAEYLDIEGMDDPKFPF 811
VA G + K + FPF
Sbjct: 439 PAAVAIGPSGRTITKEARKHLTAYGADAFPF 469
Score = 63.9 bits (154), Expect = 7e-08
Identities = 60/226 (26%), Positives = 93/226 (41%), Gaps = 6/226 (2%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK-NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPVI 196
+ T L+ YE+L K +FEVV I ++ G ++E+F F MPWLA+PF D
Sbjct: 54 NFTPLLVEVYEQLSSKGDFEVVFI------SSDG--DDESFNTYFSEMPWLAIPFSD--- 102
Query: 197 KKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDLE- 373
+ R+ + G P LV+ GK G +M G+ YPF + L+
Sbjct: 103 TETRQRLKEVFKVRG---IPHLVIFDTNGKVSCDDGVSTVMEHGVDGYPFNLDRLNFLKE 159
Query: 374 ----VRKVKEVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTFL 541
+K + + S ++ S I ND ++ ++GK + EF L
Sbjct: 160 QEENAKKNQTISSILVSSSRDYVISNDGKKIPVLDLEGKLVGLYFSAHAHRMCREFTPKL 219
Query: 542 KLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGS 679
Y E FEV+ + + D E +PWL F + S
Sbjct: 220 VELYKTLKEKRENFEVVLISLDDEEEDFKESFETMPWLALPFKDKS 265
>ref|XP_002314537.1| predicted protein [Populus trichocarpa] gi|222863577|gb|EEF00708.1|
predicted protein [Populus trichocarpa]
Length = 555
Score = 108 bits (270), Expect = 3e-21
Identities = 81/271 (29%), Positives = 123/271 (45%), Gaps = 7/271 (2%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK--NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPV 193
+ T +L+ Y+RLK+K NFEVVLI + + E+ F ++F+TMPW ALPFKD
Sbjct: 205 EFTPKLVELYKRLKEKGENFEVVLISLD--------SEEKHFKESFETMPWFALPFKDKS 256
Query: 194 IKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDL- 370
+KL R P LV++G GK P A+++ + GI+AYPFT + +L
Sbjct: 257 CEKLARYFEL-------RTIPNLVIIGQDGKTLNPNVAELIEDHGIEAYPFTPEKLEELA 309
Query: 371 EVRKVK----EVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTF 538
E+ K K ++S ++ N I S+V+ + GK ++ P FL
Sbjct: 310 EIEKAKLESQTLESVLVNGENDFVIDKSGSKVRVSDLVGKNILLYFSAQWCPPCRAFLPK 369
Query: 539 LKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGSPLLLELLHDAFGD 718
L Y+ D FEVI + S +E +++PWL F + +L G
Sbjct: 370 LIEAYHTIKAKDNAFEVIFISSDRDQSTFDEFYSEMPWLALPFGDERKQILSRKFKIQGI 429
Query: 719 ARLVAFDLHGEVVRKAEYLDIEGMDDPKFPF 811
VA G + K + + FPF
Sbjct: 430 PAAVAIGPSGRTITKEARMHLTSYGADAFPF 460
Score = 62.8 bits (151), Expect = 2e-07
Identities = 71/280 (25%), Positives = 111/280 (39%), Gaps = 9/280 (3%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK-NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPVI 196
+ T L+ YE+L K +FEVV I ++E+F F MPWLA+PF D
Sbjct: 45 NFTPLLVEVYEQLSSKGDFEVVFI--------SSDRDDESFNTYFSEMPWLAIPFSDTET 96
Query: 197 KKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDLE- 373
+K + ++ P LV+ GK G + G+ YPF + L+
Sbjct: 97 RKRLK------EVFKVRGIPNLVIFDTNGKVSCDDGVSTVKEHGVDGYPFNLDRLNFLKE 150
Query: 374 ----VRKVKEVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTFL 541
+K + + S ++ S I ND ++ ++GK + EF L
Sbjct: 151 QEENAKKNQTISSILVSSSRDYVISNDGKKIPVLDLEGKLVGLYFSIHAHRMCREFTPKL 210
Query: 542 KLEYNRRMGTDEEFEVIQV-LDSEVLSDDNEHVADVPWLVQLFDEGSPLLLELLHDAFGD 718
Y R E FEV+ + LDSE E +PW F + S L +
Sbjct: 211 VELYKRLKEKGENFEVVLISLDSEE-KHFKESFETMPWFALPFKDKSCEKLARYFELRTI 269
Query: 719 ARLVAFDLHGEVVRK--AEYLDIEGMDDPKFPFYAGGLEK 832
LV G+ + AE ++ G++ +PF LE+
Sbjct: 270 PNLVIIGQDGKTLNPNVAELIEDHGIE--AYPFTPEKLEE 307
>ref|XP_002314535.1| predicted protein [Populus trichocarpa] gi|222863575|gb|EEF00706.1|
predicted protein [Populus trichocarpa]
Length = 501
Score = 107 bits (267), Expect = 6e-21
Identities = 86/294 (29%), Positives = 128/294 (43%), Gaps = 10/294 (3%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK--NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPV 193
+ T +L+ Y+ LK+K NFEVVLI + D EE F ++F+TMPWLALPFKD
Sbjct: 214 EFTPKLVELYKTLKEKGENFEVVLISLDDE--------EEDFKESFETMPWLALPFKDKS 265
Query: 194 IKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDL- 370
+KL R P LV++G GK P A+++ GI+AYPFT + +L
Sbjct: 266 CEKLVRYFEL-------RTIPNLVIIGQDGKTLNPNVAELIEEHGIEAYPFTPEKLDELA 318
Query: 371 EVRKVK----EVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTF 538
+ K K ++S ++ N I S+V + GK ++ P FL
Sbjct: 319 AIEKAKLESQTLESVLVNGENDFLIDKSGSKVPVSDLVGKNILLYFSAQWCPPCRAFLPK 378
Query: 539 LKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGSPLLLELLHDAFGD 718
L Y+ D FEVI + S +E +++PWL F + +L G
Sbjct: 379 LIEAYHTIKRKDNAFEVIFISSDRDQSTFDEFYSEMPWLALPFGDERKQILSRKFKIQGI 438
Query: 719 ARLVAFDLHGEVVRKAEYLDIEGMDDPKFPF---YAGGLEKEIYSDLYTLFNWN 871
VA G + K + + FPF + +E+E+ WN
Sbjct: 439 PAAVAIGPSGRTITKEARMHLTAYGADAFPFTEEHLKQMEEELEEKAKGKEGWN 492
Score = 62.0 bits (149), Expect = 3e-07
Identities = 59/226 (26%), Positives = 91/226 (40%), Gaps = 6/226 (2%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDKN-FEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPVI 196
+ T L+ YE+L K FEVV I ++ G ++E+F F MPWLA+PF D
Sbjct: 54 NFTPLLVEVYEQLSSKGGFEVVFI------SSDG--DDESFNTYFSEMPWLAIPFSD--- 102
Query: 197 KKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDLE- 373
+ R+ + G P LV+ GK G + G+ YPF + L+
Sbjct: 103 TETRQRLKEVFKVRG---IPRLVIFDTNGKVSSDNGVRHVKEHGVDGYPFNLDRLNFLKE 159
Query: 374 ----VRKVKEVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTFL 541
+K + + S ++ S I ND ++ ++GK + EF L
Sbjct: 160 QEENAKKNQTISSILVSSSRDYVISNDGKKIPVLDLEGKLVGLYFSVHAHRMCGEFTPKL 219
Query: 542 KLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGS 679
Y E FEV+ + + D E +PWL F + S
Sbjct: 220 VELYKTLKEKGENFEVVLISLDDEEEDFKESFETMPWLALPFKDKS 265
>gb|AAS02080.1| protein disulfide isomerase [Quercus suber]
Length = 506
Score = 107 bits (267), Expect = 6e-21
Identities = 83/278 (29%), Positives = 125/278 (44%), Gaps = 7/278 (2%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK--NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPV 193
D T +L+ Y++LK++ +FE+VLI + D E F + F TMPWLA+PFKD
Sbjct: 215 DFTPKLVEVYKKLKERGESFEIVLISLDDE--------ENDFKEGFNTMPWLAVPFKDKC 266
Query: 194 IKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDL- 370
+KL R DLE P +VV+GP GK P A+++ GI+AYPFT + +L
Sbjct: 267 CEKLARY----FDLE---TLPTVVVIGPDGKTLNPNVAELIEEHGIEAYPFTPEKLAELA 319
Query: 371 EVRKVK----EVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTF 538
E+ K + +++ ++ + + I S+V ++ GK ++ P FL
Sbjct: 320 EIEKARLEAQTLETILVSEESDFVIDKSGSKVLVSELVGKNILLYFSAHWCPPCRAFLPK 379
Query: 539 LKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGSPLLLELLHDAFGD 718
L YN D FE+I + S +E A +PWL F + L G
Sbjct: 380 LVKAYNEIKEKDNAFEIIFISSDRDQSSFDEFFAGMPWLALPFGDKRKSFLARKFKIQGI 439
Query: 719 ARLVAFDLHGEVVRKAEYLDIEGMDDPKFPFYAGGLEK 832
VA G V K I +PF L++
Sbjct: 440 PAAVAIGPSGRTVTKEARQLITAHGADAYPFTEDHLKR 477
Score = 73.6 bits (179), Expect = 9e-11
Identities = 63/232 (27%), Positives = 101/232 (43%), Gaps = 8/232 (3%)
Frame = +2
Query: 2 PDCSYDDLTVQLMSTYERLKDK-NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALP 178
P C + T L+ YE L K +FEVV I N+E+F MPWLA+P
Sbjct: 54 PCCHF---TPNLVEVYEELLPKGDFEVVFI--------SSDRNDESFNGYLAKMPWLAIP 102
Query: 179 FKDPVIKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRS 358
F D +K + +L P L+ + GK G I+ +G+ YPFT
Sbjct: 103 FSDSETRKRLK------ELFKVRGIPNLIFLDATGKVVTNQGVRIIGEYGVDGYPFTTER 156
Query: 359 MVDLE-----VRKVKEVKSYMIWDSNTVFIRNDKSEVQFCQVDGKR--LIFLIGQCCLPE 517
+ L+ +K + + + ++ S + + ND +EV +++GK L F I + CL
Sbjct: 157 INFLKEEEENAKKNQSLSTILVHGSRSHLVSNDGNEVPIPELEGKTVGLYFSIKRLCL-- 214
Query: 518 DAEFLTFLKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDE 673
+F L Y + E FE++ + + +D E +PWL F +
Sbjct: 215 --DFTPKLVEVYKKLKERGESFEIVLISLDDEENDFKEGFNTMPWLAVPFKD 264
>emb|CAC87937.1| PDI-like protein [Quercus suber]
Length = 506
Score = 107 bits (267), Expect = 6e-21
Identities = 83/278 (29%), Positives = 125/278 (44%), Gaps = 7/278 (2%)
Frame = +2
Query: 20 DLTVQLMSTYERLKDK--NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALPFKDPV 193
D T +L+ Y++LK++ +FE+VLI + D E F + F TMPWLA+PFKD
Sbjct: 215 DFTPKLVEVYKKLKERGESFEIVLISLDDE--------ENDFKEGFNTMPWLAVPFKDKC 266
Query: 194 IKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRSMVDL- 370
+KL R DLE P +VV+GP GK P A+++ GI+AYPFT + +L
Sbjct: 267 CEKLARY----FDLE---TLPTVVVIGPDGKTLNPNVAELIEEHGIEAYPFTPEKLAELA 319
Query: 371 EVRKVK----EVKSYMIWDSNTVFIRNDKSEVQFCQVDGKRLIFLIGQCCLPEDAEFLTF 538
E+ K + +++ ++ + + I S+V ++ GK ++ P FL
Sbjct: 320 EIEKARLEAQTLETILVSEESDFVIDKSGSKVLVSELVGKNILLYFSAHWCPPCRAFLPK 379
Query: 539 LKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDEGSPLLLELLHDAFGD 718
L YN D FE+I + S +E A +PWL F + L G
Sbjct: 380 LVKAYNEIKEKDNAFEIIFISSDRDQSSFDEFFAGMPWLALPFGDKRKSFLARKFKIQGI 439
Query: 719 ARLVAFDLHGEVVRKAEYLDIEGMDDPKFPFYAGGLEK 832
VA G V K I +PF L++
Sbjct: 440 PAAVAIGPSGRTVTKEARQLITAHGADAYPFTEDHLKR 477
Score = 70.9 bits (172), Expect = 6e-10
Identities = 62/232 (26%), Positives = 100/232 (43%), Gaps = 8/232 (3%)
Frame = +2
Query: 2 PDCSYDDLTVQLMSTYERLKDK-NFEVVLIYIHDSWNTQGRTNEETFWKTFKTMPWLALP 178
P C + T L+ YE L K +FEVV I N+E+F MPWLA+P
Sbjct: 54 PCCHF---TPNLVEVYEELLPKGDFEVVFI--------SSDRNDESFNGYLAKMPWLAIP 102
Query: 179 FKDPVIKKLRRICSYPLDLEGPAPNPGLVVVGPRGKFFEPYGADILMNFGIQAYPFTRRS 358
F D +K + +L P L+ + GK G I+ +G+ YPFT
Sbjct: 103 FSDSETRKRLK------ELFKVRGIPNLIFLDATGKVVTNQGVRIIGEYGVDGYPFTTER 156
Query: 359 MVDLE-----VRKVKEVKSYMIWDSNTVFIRNDKSEVQFCQVDGKR--LIFLIGQCCLPE 517
+ L+ +K + + + ++ S + + ND + V +++GK L F I + CL
Sbjct: 157 INFLKEEEENAKKNQSLSTILVHGSRSHLVSNDGNGVPIPELEGKTVGLYFSIKRLCL-- 214
Query: 518 DAEFLTFLKLEYNRRMGTDEEFEVIQVLDSEVLSDDNEHVADVPWLVQLFDE 673
+F L Y + E FE++ + + +D E +PWL F +
Sbjct: 215 --DFTPKLVEVYKKLKERGESFEIVLISLDDEENDFKEGFNTMPWLAVPFKD 264