BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003683
         (1949 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280407.1| PREDICTED: uncharacterized protein LOC100242...   582   e-163
emb|CBI28000.3| unnamed protein product [Vitis vinifera]              545   e-152
ref|XP_002325029.1| predicted protein [Populus trichocarpa] gi|2...   520   e-145
ref|XP_002514216.1| rRNA methylase, putative [Ricinus communis] ...   518   e-144
ref|XP_004135672.1| PREDICTED: uncharacterized tRNA/rRNA methylt...   508   e-141

>ref|XP_002280407.1| PREDICTED: uncharacterized protein LOC100242436 [Vitis vinifera]
          Length = 595

 Score =  582 bits (1499), Expect = e-163
 Identities = 331/597 (55%), Positives = 397/597 (66%), Gaps = 46/597 (7%)
 Frame = -3

Query: 1845 MYCNFTKVQAFPSAFRVSSHSPKCYNTLTCSKMQTLFVGSQFKL---PNMINPCQMNTLF 1675
            MYCNFTK QAFP A RVSS  P C+      K Q L +G   K    P ++    ++  F
Sbjct: 1    MYCNFTKCQAFPLAIRVSSQ-PICFRKFHTLKTQFLPIGFHPKPSQNPILVKCATLSCGF 59

Query: 1674 NLV-QENVRVIRSPIRSLGSCHR---EGRVSKPRNYSSGLRIKHVLESRSLVGRARXXXX 1507
                 EN   ++S    +   H+   +G   +PR+ S G R+K+V++S + +        
Sbjct: 60   GCKPHENSHSLKSTGILIELHHKALEKGTTRRPRSVSCGFRLKNVVQSYNFLRGRSFSSS 119

Query: 1506 XXXXXXXXXXXXXSEKALPWL----AKSRFSD----------------AKRSQKIGATPK 1387
                         + KALPWL    AK R S                 AKRS+K+  T +
Sbjct: 120  YSVRVVGGSGAMRTGKALPWLTAKGAKERKSSDKVVNLKNNQSYWEKSAKRSEKV-TTAE 178

Query: 1386 TSRSSWEESAETFLK-----------GSXXXXXXXXXXXXXXXRSNFRKKXXXXXXXXXX 1240
            TSRSSWEESAE F K           G+                 + +            
Sbjct: 179  TSRSSWEESAERFGKNHSSRELLPKNGTLRAGTEGSKNIMVDMVESQKNGYMERDESAEE 238

Query: 1239 XXXXVNDPRWDNIRNKFGRNVDVKPRSERPEMRRWDRQEMWGKKTWKEATESTLPKMIGE 1060
                ++DPRWD I+N++    DVK R ERPE+RRW++QE WG+KTWKEATEST+PK++GE
Sbjct: 239  EMEVIDDPRWDKIKNRYTETGDVKGRYERPELRRWNKQENWGRKTWKEATESTIPKIVGE 298

Query: 1059 GVYGVGPVLAALSAGRREFYTLYTQEGLDLSTNNXXXXXXKGFEKVLRIAEKLGLSKKEI 880
            G+YGVGPVLAALSAGRREFY LY QEGLDLS NN      KGFEKVL++AEK+GLS K++
Sbjct: 299  GIYGVGPVLAALSAGRREFYALYVQEGLDLSGNNRKKKDKKGFEKVLKLAEKIGLSIKDL 358

Query: 879  SKHDLNMISDNRPHQGLVLDASPLEMVSIKELDPAPDE-EKGLLWLALDEVTDPQNLGAI 703
            SKHDLNM+ DNRPHQGLVLDASPLEMV IKELDP   E EKG LW+ALDEVTDPQNLGAI
Sbjct: 359  SKHDLNMVVDNRPHQGLVLDASPLEMVKIKELDPVLTEGEKGPLWVALDEVTDPQNLGAI 418

Query: 702  IRSAYFFGAAGVVLCAKNSAPLSGVVSKASAGSLELMELRSCRNMMQFLVSSADNGWRVL 523
            IRSAYFFG +GVVLCAKNSAPLSGVVSKASAGSLELMELR C+NMMQFL SSA+NGW+VL
Sbjct: 419  IRSAYFFGVSGVVLCAKNSAPLSGVVSKASAGSLELMELRFCKNMMQFLTSSAENGWQVL 478

Query: 522  GGSVASRAVPLNEVDSGSPTILVLGSEGTGLRPLVERSCTQLIRIPGNIPVDVSAGVEDD 343
            GGSV+ RA+PLNE+  G+PTILVLGSEGTGLRPLVERSCTQLIRIPGNIPV V+AG  +D
Sbjct: 479  GGSVSPRAIPLNEISPGAPTILVLGSEGTGLRPLVERSCTQLIRIPGNIPVHVTAGEVED 538

Query: 342  AETSKMDHKSSAEEFRSFLAVESLNVSVAAGVLLHHLIG-------IKGDNQIQEVE 193
             +TS+MD +   E+F+SFLAVESLNVSVAAGVLLHHL+G       + GD Q+  ++
Sbjct: 539  VDTSEMDRRCLTEDFQSFLAVESLNVSVAAGVLLHHLVGNSYENNCLLGDKQVDALQ 595


>emb|CBI28000.3| unnamed protein product [Vitis vinifera]
          Length = 569

 Score =  545 bits (1404), Expect = e-152
 Identities = 320/597 (53%), Positives = 379/597 (63%), Gaps = 46/597 (7%)
 Frame = -3

Query: 1845 MYCNFTKVQAFPSAFRVSSHSPKCYNTLTCSKMQTLFVGSQFKL---PNMINPCQMNTLF 1675
            MYCNFTK QAFP A RVSS  P C+      K Q L +G   K    P ++    ++  F
Sbjct: 1    MYCNFTKCQAFPLAIRVSSQ-PICFRKFHTLKTQFLPIGFHPKPSQNPILVKCATLSCGF 59

Query: 1674 NLV-QENVRVIRSPIRSLGSCHR---EGRVSKPRNYSSGLRIKHVLESRSLVGRARXXXX 1507
                 EN   ++S    +   H+   +G   +PR+ S G R+K+V++S + +        
Sbjct: 60   GCKPHENSHSLKSTGILIELHHKALEKGTTRRPRSVSCGFRLKNVVQSYNFLRGRSFSSS 119

Query: 1506 XXXXXXXXXXXXXSEKALPWL----AKSRFSD----------------AKRSQKIGATPK 1387
                         + KALPWL    AK R S                 AKRS+K+  T +
Sbjct: 120  YSVRVVGGSGAMRTGKALPWLTAKGAKERKSSDKVVNLKNNQSYWEKSAKRSEKV-TTAE 178

Query: 1386 TSRSSWEESAETFLK-----------GSXXXXXXXXXXXXXXXRSNFRKKXXXXXXXXXX 1240
            TSRSSWEESAE F K           G+                 + +            
Sbjct: 179  TSRSSWEESAERFGKNHSSRELLPKNGTLRAGTEGSKNIMVDMVESQKNGYMERDESAEE 238

Query: 1239 XXXXVNDPRWDNIRNKFGRNVDVKPRSERPEMRRWDRQEMWGKKTWKEATESTLPKMIGE 1060
                ++DPRWD I+N++    DVK R ERPE+RRW++QE WG+KTWKEATEST+PK++GE
Sbjct: 239  EMEVIDDPRWDKIKNRYTETGDVKGRYERPELRRWNKQENWGRKTWKEATESTIPKIVGE 298

Query: 1059 GVYGVGPVLAALSAGRREFYTLYTQEGLDLSTNNXXXXXXKGFEKVLRIAEKLGLSKKEI 880
            G+YGVGPVLAALSAGRREFY LY QEGLDLS NN      KGFEKVL++AEK+GLS K++
Sbjct: 299  GIYGVGPVLAALSAGRREFYALYVQEGLDLSGNNRKKKDKKGFEKVLKLAEKIGLSIKDL 358

Query: 879  SKHDLNMISDNRPHQGLVLDASPLEMVSIKELDPAPDE-EKGLLWLALDEVTDPQNLGAI 703
            SKHDLNM+ DNRPHQGLVLDASPLEMV IKELDP   E EKG LW+ALDEVTDPQNLGAI
Sbjct: 359  SKHDLNMVVDNRPHQGLVLDASPLEMVKIKELDPVLTEGEKGPLWVALDEVTDPQNLGAI 418

Query: 702  IRSAYFFGAAGVVLCAKNSAPLSGVVSKASAGSLELMELRSCRNMMQFLVSSADNGWRVL 523
            IRSAYFFG +GVVLCAKNSAPLSGVVSKASAGSLELMELR C+NMMQFL SSA+NGW+VL
Sbjct: 419  IRSAYFFGVSGVVLCAKNSAPLSGVVSKASAGSLELMELRFCKNMMQFLTSSAENGWQVL 478

Query: 522  GGSVASRAVPLNEVDSGSPTILVLGSEGTGLRPLVERSCTQLIRIPGNIPVDVSAGVEDD 343
            GGSV+ RA+PLNE+  G+PTILVLGSEGTGLRPLVERSCTQLIRIPGNIP          
Sbjct: 479  GGSVSPRAIPLNEISPGAPTILVLGSEGTGLRPLVERSCTQLIRIPGNIP---------- 528

Query: 342  AETSKMDHKSSAEEFRSFLAVESLNVSVAAGVLLHHLIG-------IKGDNQIQEVE 193
                            SFLAVESLNVSVAAGVLLHHL+G       + GD Q+  ++
Sbjct: 529  ----------------SFLAVESLNVSVAAGVLLHHLVGNSYENNCLLGDKQVDALQ 569


>ref|XP_002325029.1| predicted protein [Populus trichocarpa] gi|222866463|gb|EEF03594.1|
            predicted protein [Populus trichocarpa]
          Length = 571

 Score =  520 bits (1338), Expect = e-145
 Identities = 307/579 (53%), Positives = 366/579 (63%), Gaps = 33/579 (5%)
 Frame = -3

Query: 1845 MYCNFTKVQAFPSAFRVS--------------SHSPKCYNTLTCSKMQTLFVG---SQFK 1717
            MY N  K Q FP A RVS               HS    N L C+   TL  G      K
Sbjct: 1    MYSNLAKGQLFPLATRVSLKPRCLKPQFLPIGCHSKPSLNPL-CAHFTTLSHGFGHKAHK 59

Query: 1716 LPNMINPCQMNTLFNLVQENVRVIRSPIRSLGSCHREG----RVSKPRNYSSGLRIKHVL 1549
            + N + P + N  F          +S  RS      EG    RV K   + +  ++K   
Sbjct: 60   VRNFVRPTKTNAEF----------QSFSRSFSQRVDEGSGVVRVGKSLPWLNSKKVKEAK 109

Query: 1548 ---------ESRSLVGRA--RXXXXXXXXXXXXXXXXXSEKALPWLAKSRFSDAKRSQKI 1402
                      S S  G +  R                  +  +     S + ++K+  + 
Sbjct: 110  GLEIGASGKSSNSYRGESVKRLEKVSGDSSWVNSRKRLEKGTVAETGYSSWEESKKRLEE 169

Query: 1401 GATPKTSRSSWEESAETFLKGSXXXXXXXXXXXXXXXRSNFRKKXXXXXXXXXXXXXXVN 1222
             A  KT RSSWE+ AE                          ++              V+
Sbjct: 170  VAVGKTERSSWEDIAEA--------ASGRENIHKPSGYGYNEREDGVEGGEAEEDLEVVD 221

Query: 1221 DPRWDNIRNKFGRNVDVKPRSERPEMRRWDRQEMWGKKTWKEATESTLPKMIGEGVYGVG 1042
            DPRWD I+N+F   V V+ ++E+P+ +RW+RQ+ WG+KTWKEATE T+PK++GEGV+GVG
Sbjct: 222  DPRWDKIKNRFRGRVGVRGQTEKPDFQRWNRQDNWGRKTWKEATEFTVPKIVGEGVFGVG 281

Query: 1041 PVLAALSAGRREFYTLYTQEGLDLSTNNXXXXXXKGFEKVLRIAEKLGLSKKEISKHDLN 862
            PVLAALS GRREFY LY QEGLDLS NN      KGFEKVLR+A+K+GLS KE+SKHDLN
Sbjct: 282  PVLAALSTGRREFYALYIQEGLDLSGNNRKRKDKKGFEKVLRMAKKIGLSTKEVSKHDLN 341

Query: 861  MISDNRPHQGLVLDASPLEMVSIKELDPA-PDEEKGLLWLALDEVTDPQNLGAIIRSAYF 685
            MI DNRPHQGLVLDASPLEMV I+ELD   PDEEKG LW+ALDEVTDPQNLGAIIRSAYF
Sbjct: 342  MIVDNRPHQGLVLDASPLEMVKIQELDAVLPDEEKGSLWVALDEVTDPQNLGAIIRSAYF 401

Query: 684  FGAAGVVLCAKNSAPLSGVVSKASAGSLELMELRSCRNMMQFLVSSADNGWRVLGGSVAS 505
            FGA+GVVLCAKNSAPLSGVVSKASAGSLELMELR C+NMMQFLVSSA+NGWRVLGGSV+S
Sbjct: 402  FGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLVSSAENGWRVLGGSVSS 461

Query: 504  RAVPLNEVDSGSPTILVLGSEGTGLRPLVERSCTQLIRIPGNIPVDVSAGVEDDAETSKM 325
            +AV L+EV  G PTILVLGSEGTGLRPLVERSCTQL+RIPGNIPVD + G  D+ +  ++
Sbjct: 462  KAVSLSEVLPGQPTILVLGSEGTGLRPLVERSCTQLVRIPGNIPVDATVGGGDEVKDEEI 521

Query: 324  DHKSSAEEFRSFLAVESLNVSVAAGVLLHHLIGIKGDNQ 208
            + + S+ EFRSFLAVESLNVSVAAGVLLHHLIG   +N+
Sbjct: 522  NLECSSAEFRSFLAVESLNVSVAAGVLLHHLIGNNNENR 560


>ref|XP_002514216.1| rRNA methylase, putative [Ricinus communis]
            gi|223546672|gb|EEF48170.1| rRNA methylase, putative
            [Ricinus communis]
          Length = 517

 Score =  518 bits (1333), Expect = e-144
 Identities = 272/399 (68%), Positives = 307/399 (76%), Gaps = 1/399 (0%)
 Frame = -3

Query: 1419 KRSQKIGATPKTSRSSWEESAETFLKGSXXXXXXXXXXXXXXXRSNFRKKXXXXXXXXXX 1240
            K  +K G T +++RSSWEES E  L+ +                 N              
Sbjct: 110  KNLEKEGVT-RSARSSWEESVEPALESNRNRMVGRVKDREYDCGFNETNASVDKEEEGEG 168

Query: 1239 XXXXVNDPRWDNIRNKFGRNVDVKPRSERPEMRRWDRQEMWGKKTWKEATESTLPKMIGE 1060
                  DPRWD I+N+F    + K R+E+ E  RW++QE WG+KTW+EATESTLPKM+G 
Sbjct: 169  ELDVSEDPRWDKIKNRFNGMTNAKGRTEKTEFNRWNKQENWGRKTWREATESTLPKMVGG 228

Query: 1059 GVYGVGPVLAALSAGRREFYTLYTQEGLDLSTNNXXXXXXKGFEKVLRIAEKLGLSKKEI 880
            GVYGVGPVLAALSA RREFY LY QEGLDL TNN      KGFEKVL++A+++GLS + +
Sbjct: 229  GVYGVGPVLAALSAARREFYALYVQEGLDL-TNNKKKKDKKGFEKVLKMAQRIGLSIRRV 287

Query: 879  SKHDLNMISDNRPHQGLVLDASPLEMVSIKELDPAPDE-EKGLLWLALDEVTDPQNLGAI 703
            SKHDLNMI DNRPHQGLVLDASPLEMV I ELDP   E EKG LW+ALDEVTDPQNLGAI
Sbjct: 288  SKHDLNMIVDNRPHQGLVLDASPLEMVKIMELDPVSSEDEKGSLWVALDEVTDPQNLGAI 347

Query: 702  IRSAYFFGAAGVVLCAKNSAPLSGVVSKASAGSLELMELRSCRNMMQFLVSSADNGWRVL 523
            IRSAYFFGAAG+VLCAKNSAPLSGVVSKASAGSLE+MELR CRNMMQFLVSSADNGWRVL
Sbjct: 348  IRSAYFFGAAGIVLCAKNSAPLSGVVSKASAGSLEIMELRYCRNMMQFLVSSADNGWRVL 407

Query: 522  GGSVASRAVPLNEVDSGSPTILVLGSEGTGLRPLVERSCTQLIRIPGNIPVDVSAGVEDD 343
            GGSVAS AVPLNEV  G PTILVLGSEGTGLRPLVERSCTQL+RIPGNIP+DV+ G  D 
Sbjct: 408  GGSVASMAVPLNEVVPGEPTILVLGSEGTGLRPLVERSCTQLVRIPGNIPMDVTMGGNDV 467

Query: 342  AETSKMDHKSSAEEFRSFLAVESLNVSVAAGVLLHHLIG 226
             E+ +++ + S+E FRSFLAVESLNVSVAAGVLLHHLIG
Sbjct: 468  VESEEINLQHSSEGFRSFLAVESLNVSVAAGVLLHHLIG 506


>ref|XP_004135672.1| PREDICTED: uncharacterized tRNA/rRNA methyltransferase MAV_0574-like
            [Cucumis sativus]
          Length = 529

 Score =  508 bits (1307), Expect = e-141
 Identities = 285/498 (57%), Positives = 337/498 (67%), Gaps = 21/498 (4%)
 Frame = -3

Query: 1629 SLGSCHREGRVSKPRNYSSGLRIKH--VLESRSLVGRARXXXXXXXXXXXXXXXXXSEKA 1456
            S+GS  R   ++K R +SS  R+K   VL    L   A                      
Sbjct: 32   SVGSSLRSVSLNKSRCFSSSYRLKDEGVLSGNKLPWLAAASSVEKAKWLDKSTTKKRGDR 91

Query: 1455 LPWLAK-----------SRFSDAKRSQKIGATP--KTSRSSWEESAETFLKGSXXXXXXX 1315
             PW              +R S  +  ++ G  P    +RS+WE SAE F + S       
Sbjct: 92   SPWKESDFKSKRFGKNGNRSSRDEPEKRYGKEPIGSANRSTWEVSAEKFARRSIELADEK 151

Query: 1314 XXXXXXXXRSNFRK-----KXXXXXXXXXXXXXXVNDPRWDNIRNKFGRNVDVKPRSERP 1150
                     +  +K     +              + DPRWD I+++F   VD +  +ERP
Sbjct: 152  RESRRTRMDNEEQKDDDIERRGSYEEVEDEETEAIADPRWDKIKSRFRGMVDERGATERP 211

Query: 1149 EMRRWDRQEMWGKKTWKEATESTLPKMIGEGVYGVGPVLAALSAGRREFYTLYTQEGLDL 970
            E +RW++QE WG+KTWKEA+ESTLPKMIGEGVYGVGPVLAALSAGRREFY LY QEG+DL
Sbjct: 212  EFQRWNKQESWGRKTWKEASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYVQEGIDL 271

Query: 969  STNNXXXXXXKGFEKVLRIAEKLGLSKKEISKHDLNMISDNRPHQGLVLDASPLEMVSIK 790
            + NN      KGFEKVL++AEK  L+ KEISKHDLNMISDNRPHQGLVLDASPLEMV IK
Sbjct: 272  NNNNKKKKDKKGFEKVLKLAEKNALTIKEISKHDLNMISDNRPHQGLVLDASPLEMVKIK 331

Query: 789  ELDPAP-DEEKGLLWLALDEVTDPQNLGAIIRSAYFFGAAGVVLCAKNSAPLSGVVSKAS 613
            ELDP   +++KG LWLALDEVTDPQNLGAIIRSAYFFGA+GVVLCAKNSAPLSGVVSKAS
Sbjct: 332  ELDPVSLEDDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKAS 391

Query: 612  AGSLELMELRSCRNMMQFLVSSADNGWRVLGGSVASRAVPLNEVDSGSPTILVLGSEGTG 433
            AGSLELMELR C+NMMQFL SSA+NGWRVLG SV+S+++ L EV  G+PTILVLGSEGTG
Sbjct: 392  AGSLELMELRYCKNMMQFLTSSAENGWRVLGASVSSKSIDLKEVLPGAPTILVLGSEGTG 451

Query: 432  LRPLVERSCTQLIRIPGNIPVDVSAGVEDDAETSKMDHKSSAEEFRSFLAVESLNVSVAA 253
            LRPLVERSC+QL+RIPGNIPVDV+   E+D ET+      S +EFRSF+AVESLNVSVAA
Sbjct: 452  LRPLVERSCSQLVRIPGNIPVDVTIDTENDIETT---DGRSVDEFRSFMAVESLNVSVAA 508

Query: 252  GVLLHHLIGIKGDNQIQE 199
            GV +HHL+G    N+  E
Sbjct: 509  GVFVHHLVGNSYKNESLE 526