BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003550
         (2058 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFL91701.1| anthranilate synthase alpha-subunit 2 [Mitragyna ...   903   0.0  
emb|CAC29060.1| anthranilate synthase alpha subunit [Catharanthu...   879   0.0  
emb|CAN83610.1| hypothetical protein VITISV_035611 [Vitis vinifera]   868   0.0  
ref|XP_002279476.1| PREDICTED: anthranilate synthase component I...   867   0.0  
gb|AAA74900.1| anthranilate synthase alpha subunit [Ruta graveol...   865   0.0  

>gb|AFL91701.1| anthranilate synthase alpha-subunit 2 [Mitragyna speciosa]
          Length = 575

 Score =  903 bits (2333), Expect = 0.0
 Identities = 446/543 (82%), Positives = 487/543 (89%), Gaps = 6/543 (1%)
 Frame = -1

Query: 2049 RTLKCSALSPTSLADQSVKFKEAAKDGNLIPLYRAIFSDHLTPVLAYRCLVKEDDRDAPS 1870
            RT +CS +SP SL DQS KFKEAAK GNLIPLYR IFSDHLTPVLAYRCLVKEDDR+APS
Sbjct: 33   RTFRCSTVSPPSLVDQSAKFKEAAKHGNLIPLYRPIFSDHLTPVLAYRCLVKEDDREAPS 92

Query: 1869 FLFESVDPGLKASSVGRYSVIGAQPTMEIVAKESVVTIMDHHKGKRTEEIVDDPMVVPRR 1690
            FLFESV+PGLKASS+GRYSVIGAQPTMEI+AKE++VTIMDHH+G+R E+  +DPM++PRR
Sbjct: 93   FLFESVEPGLKASSLGRYSVIGAQPTMEIIAKENMVTIMDHHQGRRVEQFEEDPMLIPRR 152

Query: 1689 IMERWKPQRVDELPEAFCGGWVGYFSYDTVRYLEKKKMPFLNAPEDDRNLPDVHLGLYDD 1510
            IME+WKPQR++ELP AFCGGWVGYFSYDTVRY+EKKK+PF NAP DDRNLPDVHLGLYDD
Sbjct: 153  IMEKWKPQRIEELPAAFCGGWVGYFSYDTVRYVEKKKLPFSNAPIDDRNLPDVHLGLYDD 212

Query: 1509 VIVFDHVEKKAFVIHWVHMGRFSSVEEAFNDGMDRLETLVSRVHDIVPPRLSAGSIKLYT 1330
            VIVFDHVEKKA++IHWV +  FSSVEEA+NDG++RLE+LVSRVHDIVPPRL+AGSI LYT
Sbjct: 213  VIVFDHVEKKAYLIHWVRLDEFSSVEEAYNDGINRLESLVSRVHDIVPPRLAAGSINLYT 272

Query: 1329 SLFGPSLKNSSMSCEAYKEAVLQAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIV 1150
            SLFGPSLK S+M+ E Y++AVLQAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIV
Sbjct: 273  SLFGPSLKKSTMTSEDYEKAVLQAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIV 332

Query: 1149 NPSPYMTYLQARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKTPKEDYMLENQLLHD 970
            NPSPYMTYLQARGCILVASSPEILTRVKK  ITNRPLAGT RRGKTPKEDYMLE QLLHD
Sbjct: 333  NPSPYMTYLQARGCILVASSPEILTRVKKGTITNRPLAGTTRRGKTPKEDYMLETQLLHD 392

Query: 969  EKQCAEHIMLVDLGRNDVGK------VXXXXXXXXXXXXXXXXXXXXVTGELLDGLTSWD 808
            EKQCAEHIMLVDLGRNDVGK      V                    VTGELLD LTSWD
Sbjct: 393  EKQCAEHIMLVDLGRNDVGKVSKPGSVNVEKLMNIERYSHVTHISSTVTGELLDHLTSWD 452

Query: 807  ALRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISYTGDMDIALALRTIVFPT 628
             LRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGIS++GDMD+ALALRTIVFPT
Sbjct: 453  VLRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISFSGDMDVALALRTIVFPT 512

Query: 627  GMRHDTMYSYKDANKRRDWVAHLQAGAGIVADSDPADEQRECENKAAGLARAIDLAESSF 448
            G R+DTMYSYKD  KRR+W+AHLQ+GAGIVADSDP+DEQREC+NKAA LARAIDLAESSF
Sbjct: 513  GTRYDTMYSYKDEIKRREWIAHLQSGAGIVADSDPSDEQRECQNKAAALARAIDLAESSF 572

Query: 447  VKK 439
            V+K
Sbjct: 573  VEK 575


>emb|CAC29060.1| anthranilate synthase alpha subunit [Catharanthus roseus]
          Length = 608

 Score =  879 bits (2270), Expect = 0.0
 Identities = 437/540 (80%), Positives = 476/540 (88%), Gaps = 6/540 (1%)
 Frame = -1

Query: 2040 KCSALSPTSLADQSVKFKEAAKDGNLIPLYRAIFSDHLTPVLAYRCLVKEDDRDAPSFLF 1861
            KCSA+SP S  D S KFK AAK GNLIPLYR IFSDHLTPVLAYRCLVKEDDR+APSFLF
Sbjct: 69   KCSAVSPPSFVDHSAKFKVAAKHGNLIPLYRPIFSDHLTPVLAYRCLVKEDDREAPSFLF 128

Query: 1860 ESVDPGLKASSVGRYSVIGAQPTMEIVAKESVVTIMDHHKGKRTEEIVDDPMVVPRRIME 1681
            ESV+PGLK S+VGRYSVIGAQPTMEIVAKE++VT+MDH +G+R E+  +DPMVVPRRIME
Sbjct: 129  ESVEPGLKVSNVGRYSVIGAQPTMEIVAKENMVTVMDHRQGRRVEQYEEDPMVVPRRIME 188

Query: 1680 RWKPQRVDELPEAFCGGWVGYFSYDTVRYLEKKKMPFLNAPEDDRNLPDVHLGLYDDVIV 1501
            +WKPQR +ELPEAFCGGWVGYFSYDTVRY+EKKK+PF NAP DDR+LPD+HLGLYDDVIV
Sbjct: 189  KWKPQRTEELPEAFCGGWVGYFSYDTVRYVEKKKLPFSNAPVDDRDLPDIHLGLYDDVIV 248

Query: 1500 FDHVEKKAFVIHWVHMGRFSSVEEAFNDGMDRLETLVSRVHDIVPPRLSAGSIKLYTSLF 1321
            FDHVEKKA+VIHWV + +FSSVEEA+ DGM RLE+LVSRVHDIVPP+LSAGSI L+TSLF
Sbjct: 249  FDHVEKKAYVIHWVRLDQFSSVEEAYKDGMKRLESLVSRVHDIVPPKLSAGSINLHTSLF 308

Query: 1320 GPSLKNSSMSCEAYKEAVLQAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIVNPS 1141
            GP LKNS+M+ E Y++AVLQAKEHILAGDIFQ+VLSQRFERRTFADPFEVYRALRIVNPS
Sbjct: 309  GPKLKNSTMTSEEYEKAVLQAKEHILAGDIFQLVLSQRFERRTFADPFEVYRALRIVNPS 368

Query: 1140 PYMTYLQARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKTPKEDYMLENQLLHDEKQ 961
            PYMTYLQARGCILVASSPEILTRVK+  ITNRPLAGT RRGKTPKEDYMLE QLL+DEKQ
Sbjct: 369  PYMTYLQARGCILVASSPEILTRVKQGTITNRPLAGTTRRGKTPKEDYMLEQQLLNDEKQ 428

Query: 960  CAEHIMLVDLGRNDVGKV------XXXXXXXXXXXXXXXXXXXXVTGELLDGLTSWDALR 799
            CAEHIMLVDLGRNDVGKV                          VTGELLD LTSWD LR
Sbjct: 429  CAEHIMLVDLGRNDVGKVSKPGSEKVEKLMNIEPYSHVMHISSTVTGELLDNLTSWDVLR 488

Query: 798  AALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISYTGDMDIALALRTIVFPTGMR 619
            AALPVGTVSGAPKVKAMELID+LEVTRRGPYSGGFGGIS++GDMD+ALALRTIVF  G R
Sbjct: 489  AALPVGTVSGAPKVKAMELIDELEVTRRGPYSGGFGGISFSGDMDVALALRTIVFSIGTR 548

Query: 618  HDTMYSYKDANKRRDWVAHLQAGAGIVADSDPADEQRECENKAAGLARAIDLAESSFVKK 439
            +DTMYSYK   KRR+W+AHLQAGAGIVADSDPADEQRECENK+A LARAIDLAESSFV K
Sbjct: 549  YDTMYSYKTRFKRREWIAHLQAGAGIVADSDPADEQRECENKSAALARAIDLAESSFVDK 608


>emb|CAN83610.1| hypothetical protein VITISV_035611 [Vitis vinifera]
          Length = 601

 Score =  868 bits (2243), Expect = 0.0
 Identities = 431/534 (80%), Positives = 475/534 (88%), Gaps = 6/534 (1%)
 Frame = -1

Query: 2022 PTSLADQSVKFKEAAKDGNLIPLYRAIFSDHLTPVLAYRCLVKEDDRDAPSFLFESVDPG 1843
            P    D S KF EAAK GNLIPL+R+IFSDHLTPVLAYRCLVKEDDRDAPSFL+ESV+PG
Sbjct: 68   PVFAVDHSAKFFEAAKKGNLIPLHRSIFSDHLTPVLAYRCLVKEDDRDAPSFLYESVEPG 127

Query: 1842 LKASSVGRYSVIGAQPTMEIVAKESVVTIMDHHKGKRTEEIVDDPMVVPRRIMERWKPQR 1663
            +++S+VGRYSVIGAQPT+EIVAKE++VTIMDH  G RTEEIV+DPM +PRR+ME WKPQ 
Sbjct: 128  IQSSNVGRYSVIGAQPTIEIVAKENMVTIMDHEAGSRTEEIVEDPMSIPRRMMEGWKPQL 187

Query: 1662 VDELPEAFCGGWVGYFSYDTVRYLEKKKMPFLNAPEDDRNLPDVHLGLYDDVIVFDHVEK 1483
            +DELPEAFCGGWVGYFSYDTVRY+EKKK+PFL+AP+DDRNLPDVHLGLY+DV+VFDHV+K
Sbjct: 188  LDELPEAFCGGWVGYFSYDTVRYVEKKKLPFLSAPKDDRNLPDVHLGLYEDVLVFDHVKK 247

Query: 1482 KAFVIHWVHMGRFSSVEEAFNDGMDRLETLVSRVHDIVPPRLSAGSIKLYTSLFGPSLKN 1303
            + FVIHWV + ++SSVEEAFNDGM+RLE LVSRVHDIVPP+L+AGSIKL T LFGPSL+ 
Sbjct: 248  QVFVIHWVRLDQYSSVEEAFNDGMNRLEALVSRVHDIVPPKLAAGSIKLQTGLFGPSLEK 307

Query: 1302 SSMSCEAYKEAVLQAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIVNPSPYMTYL 1123
            S+M+C+ Y +AVL+AKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIVNPSPYMTYL
Sbjct: 308  STMTCDEYMKAVLEAKEHILAGDIFQIVLSQRFERRTFADPFEVYRALRIVNPSPYMTYL 367

Query: 1122 QARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKTPKEDYMLENQLLHDEKQCAEHIM 943
            QARGCILVASSPEILTRVKK KI NRPLAGT+RRGKTPKED MLENQL +DEKQCAEHIM
Sbjct: 368  QARGCILVASSPEILTRVKKNKIINRPLAGTVRRGKTPKEDIMLENQLRNDEKQCAEHIM 427

Query: 942  LVDLGRNDVGK------VXXXXXXXXXXXXXXXXXXXXVTGELLDGLTSWDALRAALPVG 781
            LVDLGRNDVGK      V                    VTGELLD LTSWDALRAALPVG
Sbjct: 428  LVDLGRNDVGKVSKPGSVTVEKLMNIERYSHVMHISSTVTGELLDHLTSWDALRAALPVG 487

Query: 780  TVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISYTGDMDIALALRTIVFPTGMRHDTMYS 601
            TVSGAPKVKAMELIDQLEVTRRGPYSGGFGGIS++GDMDIALALRTIVFP+G R DTM+S
Sbjct: 488  TVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISFSGDMDIALALRTIVFPSGSRFDTMFS 547

Query: 600  YKDANKRRDWVAHLQAGAGIVADSDPADEQRECENKAAGLARAIDLAESSFVKK 439
            YKD NKRR+WVAHLQAGAGIVADS PADEQRECENKAA LARAIDLAESSF++K
Sbjct: 548  YKDMNKRREWVAHLQAGAGIVADSVPADEQRECENKAAALARAIDLAESSFIEK 601


>ref|XP_002279476.1| PREDICTED: anthranilate synthase component I-2, chloroplastic-like
            [Vitis vinifera]
          Length = 622

 Score =  867 bits (2241), Expect = 0.0
 Identities = 432/546 (79%), Positives = 479/546 (87%), Gaps = 6/546 (1%)
 Frame = -1

Query: 2058 SRGRTLKCSALSPTSLADQSVKFKEAAKDGNLIPLYRAIFSDHLTPVLAYRCLVKEDDRD 1879
            S G  +  +   P    D S KF EAAK GNLIPL+R+IFSDHLTPVLAYRCLVKEDDRD
Sbjct: 77   SLGNCVGFNWCDPVFAVDHSAKFFEAAKKGNLIPLHRSIFSDHLTPVLAYRCLVKEDDRD 136

Query: 1878 APSFLFESVDPGLKASSVGRYSVIGAQPTMEIVAKESVVTIMDHHKGKRTEEIVDDPMVV 1699
            APSFL+ESV+PG+++S+VGRYSVIGAQPT+EIVAKE++VTIMDH  G RTEEIV+DPM +
Sbjct: 137  APSFLYESVEPGIQSSNVGRYSVIGAQPTIEIVAKENMVTIMDHEAGSRTEEIVEDPMSI 196

Query: 1698 PRRIMERWKPQRVDELPEAFCGGWVGYFSYDTVRYLEKKKMPFLNAPEDDRNLPDVHLGL 1519
            PRR+ME WKPQ +DELPEAFCGGWVGYFSYDTVRY+EKKK+PFL+AP+DDRNLPDVHLGL
Sbjct: 197  PRRMMEGWKPQLLDELPEAFCGGWVGYFSYDTVRYVEKKKLPFLSAPKDDRNLPDVHLGL 256

Query: 1518 YDDVIVFDHVEKKAFVIHWVHMGRFSSVEEAFNDGMDRLETLVSRVHDIVPPRLSAGSIK 1339
            Y+DV+VFDHV+K+ FVIHWV + ++SSVEEAFNDGM+RLE LVSRVHDIVPP+L+AGSIK
Sbjct: 257  YEDVLVFDHVKKQVFVIHWVRLDQYSSVEEAFNDGMNRLEALVSRVHDIVPPKLAAGSIK 316

Query: 1338 LYTSLFGPSLKNSSMSCEAYKEAVLQAKEHILAGDIFQIVLSQRFERRTFADPFEVYRAL 1159
            L T LFGPSL+ S+M+C+ Y +AVL+AKEHILAGDIFQIVLSQRFERRTFADPFEVYRAL
Sbjct: 317  LQTGLFGPSLEKSTMTCDEYMKAVLEAKEHILAGDIFQIVLSQRFERRTFADPFEVYRAL 376

Query: 1158 RIVNPSPYMTYLQARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKTPKEDYMLENQL 979
            RIVNPSPYMTYLQARGCILVASSPEILTRVKK +I NRPLAGT+RRGKTPKED MLENQL
Sbjct: 377  RIVNPSPYMTYLQARGCILVASSPEILTRVKKNRIINRPLAGTVRRGKTPKEDIMLENQL 436

Query: 978  LHDEKQCAEHIMLVDLGRNDVGK------VXXXXXXXXXXXXXXXXXXXXVTGELLDGLT 817
             +DEKQCAEHIMLVDLGRNDVGK      V                    VTGELLD LT
Sbjct: 437  RNDEKQCAEHIMLVDLGRNDVGKVSKPGSVTVEKLMNIERYSHVMHISSTVTGELLDHLT 496

Query: 816  SWDALRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISYTGDMDIALALRTIV 637
            SWDALRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGIS++GDMDIALALRTIV
Sbjct: 497  SWDALRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISFSGDMDIALALRTIV 556

Query: 636  FPTGMRHDTMYSYKDANKRRDWVAHLQAGAGIVADSDPADEQRECENKAAGLARAIDLAE 457
            FP+G R DTM+SYKD NKRR+WVAHLQAGAGIVADS PADEQRECENKAA LARAIDLAE
Sbjct: 557  FPSGSRFDTMFSYKDMNKRREWVAHLQAGAGIVADSVPADEQRECENKAAALARAIDLAE 616

Query: 456  SSFVKK 439
            SSF++K
Sbjct: 617  SSFIEK 622


>gb|AAA74900.1| anthranilate synthase alpha subunit [Ruta graveolens]
          Length = 608

 Score =  865 bits (2234), Expect = 0.0
 Identities = 434/557 (77%), Positives = 479/557 (85%), Gaps = 18/557 (3%)
 Frame = -1

Query: 2055 RGRTLKCSALSPTS------------LADQSVKFKEAAKDGNLIPLYRAIFSDHLTPVLA 1912
            R RTLKC+A + TS            L DQS  F EA+K GNLIPLYR IFSDHLTPVLA
Sbjct: 52   RLRTLKCAASASTSASTSASPSPSPSLVDQSANFHEASKKGNLIPLYRCIFSDHLTPVLA 111

Query: 1911 YRCLVKEDDRDAPSFLFESVDPGLKASSVGRYSVIGAQPTMEIVAKESVVTIMDHHKGKR 1732
            YRCLVKEDDRDAPSFLFESV+PG +ASS+GRYSV+GAQP +EIVAKE++VTI+DH  G+R
Sbjct: 112  YRCLVKEDDRDAPSFLFESVEPGSQASSIGRYSVVGAQPAIEIVAKENMVTILDHEGGQR 171

Query: 1731 TEEIVDDPMVVPRRIMERWKPQRVDELPEAFCGGWVGYFSYDTVRYLEKKKMPFLNAPED 1552
            TE+ V+DPM VPRRIME WKPQ +DELPEAFCGGWVGYFSYDTVRY+EKKK+PF +AP D
Sbjct: 172  TEQFVEDPMDVPRRIMEGWKPQLIDELPEAFCGGWVGYFSYDTVRYVEKKKLPFFSAPTD 231

Query: 1551 DRNLPDVHLGLYDDVIVFDHVEKKAFVIHWVHMGRFSSVEEAFNDGMDRLETLVSRVHDI 1372
            DRNLPDVHLGLYDDVIVFDHVEKKAFVIHWV + ++SSV EA+NDGM+RLE LVSRVHDI
Sbjct: 232  DRNLPDVHLGLYDDVIVFDHVEKKAFVIHWVRLDQYSSVAEAYNDGMNRLENLVSRVHDI 291

Query: 1371 VPPRLSAGSIKLYTSLFGPSLKNSSMSCEAYKEAVLQAKEHILAGDIFQIVLSQRFERRT 1192
            VPP+L +GSIKL+T  FGP L+ SSM+ EAYKEAVL+AKEHILAGDIFQIVLSQRFERRT
Sbjct: 292  VPPKLRSGSIKLHTRHFGPKLERSSMTSEAYKEAVLEAKEHILAGDIFQIVLSQRFERRT 351

Query: 1191 FADPFEVYRALRIVNPSPYMTYLQARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKT 1012
            FADPFE+YR+LRIVNPSPYMTYLQARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKT
Sbjct: 352  FADPFEIYRSLRIVNPSPYMTYLQARGCILVASSPEILTRVKKRKITNRPLAGTIRRGKT 411

Query: 1011 PKEDYMLENQLLHDEKQCAEHIMLVDLGRNDVGK------VXXXXXXXXXXXXXXXXXXX 850
             KED + E +LL+DEKQCAEHIMLVDLGRNDVGK      V                   
Sbjct: 412  RKEDLVFEKELLNDEKQCAEHIMLVDLGRNDVGKVSEPGSVKVEKLMNIEHYSHVMHISS 471

Query: 849  XVTGELLDGLTSWDALRAALPVGTVSGAPKVKAMELIDQLEVTRRGPYSGGFGGISYTGD 670
             VTGELLD LTSWDALRAALPVGTVSGAPKVKAME+ID+LEVTRRGPY GGFGGIS+TGD
Sbjct: 472  TVTGELLDHLTSWDALRAALPVGTVSGAPKVKAMEIIDKLEVTRRGPYGGGFGGISFTGD 531

Query: 669  MDIALALRTIVFPTGMRHDTMYSYKDANKRRDWVAHLQAGAGIVADSDPADEQRECENKA 490
            +DIALALRT+VF T  R+DTMYSYKD +KRR+W+AHLQAGAGIVADSDPADEQRECENKA
Sbjct: 532  LDIALALRTMVFQTATRYDTMYSYKDVDKRREWIAHLQAGAGIVADSDPADEQRECENKA 591

Query: 489  AGLARAIDLAESSFVKK 439
            A LARAIDLAESSF++K
Sbjct: 592  AALARAIDLAESSFIEK 608