BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003541
         (1811 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002518000.1| diacylglycerol kinase, alpha, putative [Rici...   751   0.0  
ref|XP_003526295.1| PREDICTED: diacylglycerol kinase iota-like [...   744   0.0  
ref|NP_565492.1| diacylglycerol kinase 5 [Arabidopsis thaliana] ...   743   0.0  
gb|AAM62810.1| putative diacylglycerol kinase [Arabidopsis thali...   741   0.0  
gb|AAG23129.1|AF198259_1 diacylglycerol kinase [Solanum lycopers...   739   0.0  

>ref|XP_002518000.1| diacylglycerol kinase, alpha, putative [Ricinus communis]
            gi|223542982|gb|EEF44518.1| diacylglycerol kinase, alpha,
            putative [Ricinus communis]
          Length = 490

 Score =  751 bits (1940), Expect = 0.0
 Identities = 361/487 (74%), Positives = 410/487 (84%), Gaps = 3/487 (0%)
 Frame = -1

Query: 1631 NFASDSFLKDFYIPDYLLGAGTKGENLND---VPSCPVLVFINSKSGGQLGGDLLTTYRS 1461
            +F  +S  K+F IP ++        +++     P CPVLVFINSKSGGQLGGDLL TYRS
Sbjct: 4    DFDLESIKKEFGIPSFIFDPELFENDVDSDAPPPDCPVLVFINSKSGGQLGGDLLLTYRS 63

Query: 1460 ILNPNQVHDLGEETPDKVLRRLYVALEKLRVNGDELAPKIQERLRLIVAGGDGTAGWLLG 1281
            +LN  QV DLGEE PDKVL+R+YV LE L+  G +LA  IQ+RLR+IVAGGDGTAGWLLG
Sbjct: 64   LLNEKQVFDLGEEAPDKVLQRIYVNLEILKSRGIQLAIHIQKRLRIIVAGGDGTAGWLLG 123

Query: 1280 VVCDLKLPYPPPIATVPLGTGNNLPFAFGWGKKNPGTDRNSVLSFLMQVKGAKEMKIDSW 1101
            VVCDLK P+PPPIATVPLGTGNNLPFAFGWGKKNPGT  +SVLSFL QVK AKEMKID+W
Sbjct: 124  VVCDLKFPHPPPIATVPLGTGNNLPFAFGWGKKNPGTYSSSVLSFLNQVKKAKEMKIDNW 183

Query: 1100 HILLRMKVPKEGACDPIAPLDLPHSLHAFHRVSSSDDLNKEGCHTFRGGFWNYFSMGMDA 921
            HIL+RM+ P++G+CDPIAPL+LPHSLHAFHRVS++D+LN EG HTFRGGFWNYFSMGMDA
Sbjct: 184  HILMRMRAPRQGSCDPIAPLELPHSLHAFHRVSANDELNLEGYHTFRGGFWNYFSMGMDA 243

Query: 920  QVSYAFHSERKLHPEKFKNQLNNQKTYAKLGCTQGWFSASLSHPSSRNIAQLAKVMIMRR 741
            QVSYAFHSERKLHPEKFKNQL NQ TYAKLGCTQGWF ASL HPSS+NIAQLAKV IM+R
Sbjct: 244  QVSYAFHSERKLHPEKFKNQLVNQSTYAKLGCTQGWFMASLFHPSSKNIAQLAKVKIMKR 303

Query: 740  HGGWKELTIPHYIRSIICLNLPSFSGGLNPWGTPNKNKLRDRELTPPYVDDSLLEIVGFK 561
            HG W++L IP  IRSI+CLNLPSFSGGL+PWGTPN  K RDR+LTPPYVDD LLE+VGF+
Sbjct: 304  HGQWQDLHIPRSIRSIVCLNLPSFSGGLSPWGTPNSKKQRDRDLTPPYVDDGLLEVVGFR 363

Query: 560  DAWHGLVLLAPKGHGTRLAQAHKVKFEFHKGAANETFMRIDGEPWKQPLPVDDDTVVVEI 381
            DAWHGLVLLAP GHGTRLAQAH+++FEFHKGAA+ TFMRIDGEPWKQPLP DDDTVVVEI
Sbjct: 364  DAWHGLVLLAPNGHGTRLAQAHRIRFEFHKGAADHTFMRIDGEPWKQPLPEDDDTVVVEI 423

Query: 380  SPLGQVKMLATPECRAKSVYDQSTPTSHXXXXXXDSNGEDPVDEGSRKFGAADTFKIPDE 201
            S LGQV MLAT +C+++SVYD S+P+SH          E    E  +KFGAADTFKIPD 
Sbjct: 424  SHLGQVNMLATHDCKSRSVYDPSSPSSHDDGDDDSDKEESEHGEERKKFGAADTFKIPDG 483

Query: 200  VDIAHLS 180
            ++IAHLS
Sbjct: 484  IEIAHLS 490


>ref|XP_003526295.1| PREDICTED: diacylglycerol kinase iota-like [Glycine max]
          Length = 484

 Score =  744 bits (1920), Expect = 0.0
 Identities = 358/485 (73%), Positives = 405/485 (83%), Gaps = 3/485 (0%)
 Frame = -1

Query: 1625 ASDSFLKDFYIPDYLLGAGTKG---ENLNDVPSCPVLVFINSKSGGQLGGDLLTTYRSIL 1455
            AS  FL +F IPD++L  G+     EN+ DVP CPVLVF+NSKSGGQLGG LL TYR +L
Sbjct: 2    ASHQFLDNFIIPDHILVPGSSDSDVENVGDVPHCPVLVFVNSKSGGQLGGHLLKTYRDLL 61

Query: 1454 NPNQVHDLGEETPDKVLRRLYVALEKLRVNGDELAPKIQERLRLIVAGGDGTAGWLLGVV 1275
            NP QV DLGE  PDKVLR +Y  LE L V G + A KI+ERL+LIVAGGDGTAGWLLGVV
Sbjct: 62   NPKQVFDLGEHAPDKVLRTVYANLEGLNVRGYQFADKIKERLKLIVAGGDGTAGWLLGVV 121

Query: 1274 CDLKLPYPPPIATVPLGTGNNLPFAFGWGKKNPGTDRNSVLSFLMQVKGAKEMKIDSWHI 1095
            CDLKL +PPPIATVPLGTGNNLPFAFGWGKKNP TD+ S+ +FL QV  A +MKID+WHI
Sbjct: 122  CDLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPATDQRSIEAFLDQVMKATKMKIDNWHI 181

Query: 1094 LLRMKVPKEGACDPIAPLDLPHSLHAFHRVSSSDDLNKEGCHTFRGGFWNYFSMGMDAQV 915
            L+RM+ PKEG CDPI PL+LPHSLHAFHRVS SD+ N EGCHTFRGGFWNYFSMGMDAQV
Sbjct: 182  LMRMRAPKEGPCDPIPPLELPHSLHAFHRVSESDEFNMEGCHTFRGGFWNYFSMGMDAQV 241

Query: 914  SYAFHSERKLHPEKFKNQLNNQKTYAKLGCTQGWFSASLSHPSSRNIAQLAKVMIMRRHG 735
            SYAFHSERK +PEKFKNQL NQ TYAKLGC+QGWF AS+SHP+ RNIAQLAKV  M+RHG
Sbjct: 242  SYAFHSERKKNPEKFKNQLINQTTYAKLGCSQGWFFASMSHPADRNIAQLAKVKFMKRHG 301

Query: 734  GWKELTIPHYIRSIICLNLPSFSGGLNPWGTPNKNKLRDRELTPPYVDDSLLEIVGFKDA 555
             W++L IP  IRSI+CLNLPSFSGG NPWGTPN+ K  DR+LTPP+VDD LLEIVGF++A
Sbjct: 302  EWQDLDIPPSIRSIVCLNLPSFSGGFNPWGTPNRRKQSDRDLTPPFVDDGLLEIVGFRNA 361

Query: 554  WHGLVLLAPKGHGTRLAQAHKVKFEFHKGAANETFMRIDGEPWKQPLPVDDDTVVVEISP 375
            WHGLVLLAPKGHGTRLAQAH+++FEF KGAA+ TFMRIDGEPWKQPLPVDDDTV+VEIS 
Sbjct: 362  WHGLVLLAPKGHGTRLAQAHRIQFEFRKGAADHTFMRIDGEPWKQPLPVDDDTVMVEISH 421

Query: 374  LGQVKMLATPECRAKSVYDQSTPTSHXXXXXXDSNGEDPVDEGSRKFGAADTFKIPDEVD 195
             GQV ML+T  C++KSVYD S+P  H      DS+ ED V E  RKFGAADTF+IPDEVD
Sbjct: 422  HGQVNMLSTHNCKSKSVYDPSSP--HHEDEEDDSDEEDSVAEEFRKFGAADTFRIPDEVD 479

Query: 194  IAHLS 180
            ++HLS
Sbjct: 480  VSHLS 484


>ref|NP_565492.1| diacylglycerol kinase 5 [Arabidopsis thaliana]
            gi|42570849|ref|NP_973498.1| diacylglycerol kinase 5
            [Arabidopsis thaliana] gi|20197681|gb|AAD20931.2|
            putative diacylglycerol kinase [Arabidopsis thaliana]
            gi|222423216|dbj|BAH19585.1| AT2G20900 [Arabidopsis
            thaliana] gi|222424359|dbj|BAH20135.1| AT2G20900
            [Arabidopsis thaliana] gi|330252000|gb|AEC07094.1|
            diacylglycerol kinase 5 [Arabidopsis thaliana]
            gi|330252001|gb|AEC07095.1| diacylglycerol kinase 5
            [Arabidopsis thaliana]
          Length = 491

 Score =  743 bits (1918), Expect = 0.0
 Identities = 362/494 (73%), Positives = 417/494 (84%), Gaps = 6/494 (1%)
 Frame = -1

Query: 1643 METMNFASDSFLKDFYIPDYLLGAGTKGENLND---VPSCPVLVFINSKSGGQLGGDLLT 1473
            ME  N  SD FLK+FYIP Y+L A T+ E   +    P+ PVLVFINSKSGGQLGG+L+ 
Sbjct: 1    MEKYNSLSD-FLKEFYIPTYVLSAETEEEEEEESRPTPASPVLVFINSKSGGQLGGELIL 59

Query: 1472 TYRSILNPNQVHDLGEETPDKVLRRLYVALEKLRVNGDELAPKIQERLRLIVAGGDGTAG 1293
            TYRS+LN NQV DL +ETPDKVLRR+Y+ LE+L+   D+ A +I+E+L++IVAGGDGTAG
Sbjct: 60   TYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLK--DDDFARQIREKLKIIVAGGDGTAG 117

Query: 1292 WLLGVVCDLKLPYPPPIATVPLGTGNNLPFAFGWGKKNPGTDRNSVLSFLMQVKGAKEMK 1113
            WLLGVVCDLKL +PPPIATVPLGTGNNLPFAFGWGKKNPGTDR +V SFL QV  AK MK
Sbjct: 118  WLLGVVCDLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMK 177

Query: 1112 IDSWHILLRMKVPKEG-ACDPIAPLDLPHSLHAFHRVSSSDDLNKEGCHTFRGGFWNYFS 936
            ID+WHIL+RMK PKEG +CDP+APL+LPHSLHAFHRVS +D+LNKEGCHTFRGGFWNYFS
Sbjct: 178  IDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFS 237

Query: 935  MGMDAQVSYAFHSERKLHPEKFKNQLNNQKTYAKLGCTQGWFSASLSHPSSRNIAQLAKV 756
            +GMDAQ+SYAFHSERKLHPEKFKNQL NQ TY KLGCTQGWF ASL HP+SRNIAQLAKV
Sbjct: 238  LGMDAQISYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKV 297

Query: 755  MIMRRHGGWKELTIPHYIRSIICLNLPSFSGGLNPWGTPNKNKLRDRELTPPYVDDSLLE 576
             I  R+G W++L IPH IRSI+CLNLPSFSGGLNPWGTPN  K RDR LTPP+VDD L+E
Sbjct: 298  KIATRNGQWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIE 357

Query: 575  IVGFKDAWHGLVLLAPKGHGTRLAQAHKVKFEFHKGAANETFMRIDGEPWKQPLPVDDDT 396
            +VGF++AWHGLVLLAP GHGTRLAQA++++FEFHKGA + TFMR+DGEPWKQPLP+DD+T
Sbjct: 358  VVGFRNAWHGLVLLAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDET 417

Query: 395  VVVEISPLGQVKMLATPECRAKSVYDQSTPTSHXXXXXXDSNGEDPVDEGS--RKFGAAD 222
            V+VEIS LGQV MLAT +CR++SV+D STP         D N +D V EG   RKFGAAD
Sbjct: 418  VMVEISHLGQVNMLATHDCRSRSVFDPSTPRHQDGAEDYDDNEDDSVAEGEEFRKFGAAD 477

Query: 221  TFKIPDEVDIAHLS 180
            TFKIPDEVDI+ LS
Sbjct: 478  TFKIPDEVDISQLS 491


>gb|AAM62810.1| putative diacylglycerol kinase [Arabidopsis thaliana]
          Length = 491

 Score =  741 bits (1913), Expect = 0.0
 Identities = 362/494 (73%), Positives = 416/494 (84%), Gaps = 6/494 (1%)
 Frame = -1

Query: 1643 METMNFASDSFLKDFYIPDYLLGAGTKGENLND---VPSCPVLVFINSKSGGQLGGDLLT 1473
            ME  N  SD FLK+FYIP Y+L A T+ E   +    P+ PVLVFINSKSGGQLGG+L+ 
Sbjct: 1    MEKYNSLSD-FLKEFYIPTYVLSAETEEEEEQESRPTPASPVLVFINSKSGGQLGGELIL 59

Query: 1472 TYRSILNPNQVHDLGEETPDKVLRRLYVALEKLRVNGDELAPKIQERLRLIVAGGDGTAG 1293
            TYRS+LN NQV DL +ETPDKVLRR+Y+ LE+L+   D+ A +I E+L++IVAGGDGTAG
Sbjct: 60   TYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLK--DDDSARQIGEKLKIIVAGGDGTAG 117

Query: 1292 WLLGVVCDLKLPYPPPIATVPLGTGNNLPFAFGWGKKNPGTDRNSVLSFLMQVKGAKEMK 1113
            WLLGVVCDLKL +PPPIATVPLGTGNNLPFAFGWGKKNPGTDR +V SFL QV  AK MK
Sbjct: 118  WLLGVVCDLKLSHPPPIATVPLGTGNNLPFAFGWGKKNPGTDRTAVESFLEQVLKAKVMK 177

Query: 1112 IDSWHILLRMKVPKEG-ACDPIAPLDLPHSLHAFHRVSSSDDLNKEGCHTFRGGFWNYFS 936
            ID+WHIL+RMK PKEG +CDP+APL+LPHSLHAFHRVS +D+LNKEGCHTFRGGFWNYFS
Sbjct: 178  IDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAFHRVSPTDELNKEGCHTFRGGFWNYFS 237

Query: 935  MGMDAQVSYAFHSERKLHPEKFKNQLNNQKTYAKLGCTQGWFSASLSHPSSRNIAQLAKV 756
            +GMDAQ+SYAFHSERKLHPEKFKNQL NQ TY KLGCTQGWF ASL HP+SRNIAQLAKV
Sbjct: 238  LGMDAQISYAFHSERKLHPEKFKNQLVNQSTYVKLGCTQGWFCASLFHPASRNIAQLAKV 297

Query: 755  MIMRRHGGWKELTIPHYIRSIICLNLPSFSGGLNPWGTPNKNKLRDRELTPPYVDDSLLE 576
             I  R+G W++L IPH IRSI+CLNLPSFSGGLNPWGTPN  K RDR LTPP+VDD L+E
Sbjct: 298  KIATRNGQWQDLHIPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIE 357

Query: 575  IVGFKDAWHGLVLLAPKGHGTRLAQAHKVKFEFHKGAANETFMRIDGEPWKQPLPVDDDT 396
            +VGF++AWHGLVLLAP GHGTRLAQA++++FEFHKGA + TFMR+DGEPWKQPLP+DD+T
Sbjct: 358  VVGFRNAWHGLVLLAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDET 417

Query: 395  VVVEISPLGQVKMLATPECRAKSVYDQSTPTSHXXXXXXDSNGEDPVDEGS--RKFGAAD 222
            V+VEIS LGQV MLAT +CR++SV+D STP         D N +D V EG   RKFGAAD
Sbjct: 418  VMVEISHLGQVNMLATHDCRSRSVFDPSTPRHQDGAEDYDDNEDDSVAEGEEFRKFGAAD 477

Query: 221  TFKIPDEVDIAHLS 180
            TFKIPDEVDI+ LS
Sbjct: 478  TFKIPDEVDISQLS 491


>gb|AAG23129.1|AF198259_1 diacylglycerol kinase [Solanum lycopersicum]
          Length = 489

 Score =  739 bits (1909), Expect = 0.0
 Identities = 353/478 (73%), Positives = 407/478 (85%), Gaps = 2/478 (0%)
 Frame = -1

Query: 1607 KDFYIPDYLLGAGTKGENLN--DVPSCPVLVFINSKSGGQLGGDLLTTYRSILNPNQVHD 1434
            +DFYIP Y+L       +L   DVP+CPVLVF+NSKSGGQLGG+LL T+R +LN  QV D
Sbjct: 13   RDFYIPTYILAPNASSNSLRLPDVPTCPVLVFVNSKSGGQLGGELLRTFRHLLNKYQVFD 72

Query: 1433 LGEETPDKVLRRLYVALEKLRVNGDELAPKIQERLRLIVAGGDGTAGWLLGVVCDLKLPY 1254
            LG+E PD VLRRLY+ +E+L+ NGD  A +I+ER+++IVAGGDGTAGWLLGVV DLKL  
Sbjct: 73   LGDEAPDSVLRRLYLNIERLKGNGDHFAAEIEERMKIIVAGGDGTAGWLLGVVSDLKLSQ 132

Query: 1253 PPPIATVPLGTGNNLPFAFGWGKKNPGTDRNSVLSFLMQVKGAKEMKIDSWHILLRMKVP 1074
            PPPIATVPLGTGNNLPFAFGWGKKNPGTD NSV+SFL QV  AKEMK+DSWHIL+RM+ P
Sbjct: 133  PPPIATVPLGTGNNLPFAFGWGKKNPGTDLNSVISFLKQVMNAKEMKMDSWHILMRMRAP 192

Query: 1073 KEGACDPIAPLDLPHSLHAFHRVSSSDDLNKEGCHTFRGGFWNYFSMGMDAQVSYAFHSE 894
            K G+CDP+APL+LPHSLHAFHRVS SD+LN EG HTFRGGFWNYFSMGMDAQVSYAFHSE
Sbjct: 193  KVGSCDPVAPLELPHSLHAFHRVSPSDELNVEGFHTFRGGFWNYFSMGMDAQVSYAFHSE 252

Query: 893  RKLHPEKFKNQLNNQKTYAKLGCTQGWFSASLSHPSSRNIAQLAKVMIMRRHGGWKELTI 714
            RK++P+KFKNQL NQ +Y KLGCTQGWF A L HPSS+NIAQL KV IM++ GGW++L I
Sbjct: 253  RKMNPDKFKNQLVNQSSYLKLGCTQGWFFAPLIHPSSKNIAQLTKVKIMKKQGGWQDLHI 312

Query: 713  PHYIRSIICLNLPSFSGGLNPWGTPNKNKLRDRELTPPYVDDSLLEIVGFKDAWHGLVLL 534
            P  +RSI+CLNLPSFSGGLNPWGTPN NK R R+LTPP+VDD LLE+VGF+DAWHGLVLL
Sbjct: 313  PPSVRSIVCLNLPSFSGGLNPWGTPNSNKRRYRDLTPPFVDDGLLEVVGFRDAWHGLVLL 372

Query: 533  APKGHGTRLAQAHKVKFEFHKGAANETFMRIDGEPWKQPLPVDDDTVVVEISPLGQVKML 354
            APKGHGTRLAQAH ++FEF KGAA+ TFMRIDGEPWKQPLP +DDTVVVEIS LGQVKML
Sbjct: 373  APKGHGTRLAQAHGIRFEFQKGAADHTFMRIDGEPWKQPLPENDDTVVVEISHLGQVKML 432

Query: 353  ATPECRAKSVYDQSTPTSHXXXXXXDSNGEDPVDEGSRKFGAADTFKIPDEVDIAHLS 180
            AT +CRAKS++D S+  SH      DS+ E+ V+E  RKFGAADTFKIPDEVD++ LS
Sbjct: 433  ATHDCRAKSIHDPSSQFSH-ETDEVDSDEENSVNEEQRKFGAADTFKIPDEVDVSSLS 489