BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003516
         (828 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACD40019.1| DNase 2 [Eucommia ulmoides] gi|187942425|gb|ACD40...   391   e-106
gb|ABA41005.1| DNase 1 [Eucommia ulmoides] gi|187942423|gb|ACD40...   390   e-106
ref|XP_002311254.1| predicted protein [Populus trichocarpa] gi|2...   389   e-106
gb|AAK52082.1| nuclease [Nicotiana tabacum]                           383   e-104
gb|AAX21543.1| Ca(2+)-dependent DNase [Populus tomentosa]             382   e-104

>gb|ACD40019.1| DNase 2 [Eucommia ulmoides] gi|187942425|gb|ACD40021.1| DNase 2
           [Eucommia ulmoides]
          Length = 328

 Score =  391 bits (1004), Expect = e-106
 Identities = 189/276 (68%), Positives = 213/276 (77%)
 Frame = +1

Query: 1   ANWYRKLSVTWRESNPPPITPEEAARLIIQTLKNHLKADVEGLLAYYGLPRPHSLVELSS 180
           ANWY+K+S  WRE+ PPP TPEE +RL+I TLK H KADVEG+L++YGLP PH+LVE+S 
Sbjct: 61  ANWYKKISEAWREAKPPPRTPEEVSRLVILTLKRHQKADVEGILSFYGLPLPHTLVEISH 120

Query: 181 GEPQSRPEGLKFELHTLPVDVKAVADGDTVTVYVSTMDPRESXXXXXXXXXXXXXXXXXX 360
           G P  +PEGLKFEL TLPVD KAVADGDT+TVYV+T+DPRES                  
Sbjct: 121 GTPPLQPEGLKFELQTLPVDAKAVADGDTITVYVNTIDPRESSSIPREVQMAAVQRSKAR 180

Query: 361 XXXXXXXXXXXXXXXXXEADALHKKITDMGYRVLNINNQEVLAKKYRIRLRGIDAPESKM 540
                            +AD L KKI D GYRVL I N++VLAKKYRIRLRGIDAPES M
Sbjct: 181 SKKNYP-----------KADELQKKIVDAGYRVLTIQNEDVLAKKYRIRLRGIDAPESSM 229

Query: 541 PYGPEAKEELVKIVQGKCLKVLVFTEDRYGRCVGDIYCNGIFVQESMLKKGLVWHYTAYD 720
           PYG EAKEEL K+VQGKCL+VLVFTEDRYGRCVGDIYC+GIFVQE MLKKG  WHYTAYD
Sbjct: 230 PYGKEAKEELTKLVQGKCLRVLVFTEDRYGRCVGDIYCDGIFVQEIMLKKGYAWHYTAYD 289

Query: 721 KRPDLDKWEKEARAKRIGLWASSNPEKPWEWRKNRR 828
           +RP+L+KWEKEARAKRIGLWASSNPE PWEWRK+RR
Sbjct: 290 RRPELEKWEKEARAKRIGLWASSNPEMPWEWRKDRR 325


>gb|ABA41005.1| DNase 1 [Eucommia ulmoides] gi|187942423|gb|ACD40020.1| DNase 1
           [Eucommia ulmoides]
          Length = 328

 Score =  390 bits (1002), Expect = e-106
 Identities = 189/276 (68%), Positives = 213/276 (77%)
 Frame = +1

Query: 1   ANWYRKLSVTWRESNPPPITPEEAARLIIQTLKNHLKADVEGLLAYYGLPRPHSLVELSS 180
           ANWY+K+S  WRE+ PPP TPEE +RL+I TLK H KADVEGLL++YGLP PH+LVE+S 
Sbjct: 61  ANWYKKISEAWREAKPPPRTPEEVSRLVILTLKRHQKADVEGLLSFYGLPLPHTLVEISH 120

Query: 181 GEPQSRPEGLKFELHTLPVDVKAVADGDTVTVYVSTMDPRESXXXXXXXXXXXXXXXXXX 360
           G P  +PEGLKFEL TLPVD KAVADGDT+TVYV+T+DPRES                  
Sbjct: 121 GTPPLQPEGLKFELQTLPVDAKAVADGDTITVYVNTIDPRESSSIPREVQMAAVQRSKAR 180

Query: 361 XXXXXXXXXXXXXXXXXEADALHKKITDMGYRVLNINNQEVLAKKYRIRLRGIDAPESKM 540
                            +AD L KKI D GYRVL I N++VLAKKYRIRLRGIDAPES M
Sbjct: 181 SKKNYP-----------KADELQKKIVDAGYRVLTIQNEDVLAKKYRIRLRGIDAPESSM 229

Query: 541 PYGPEAKEELVKIVQGKCLKVLVFTEDRYGRCVGDIYCNGIFVQESMLKKGLVWHYTAYD 720
           PYG EAKEEL K+VQGKCL+VLVFTEDRYGRCVGDIYC+GIFVQ+ MLKKG  WHYTAYD
Sbjct: 230 PYGKEAKEELTKLVQGKCLRVLVFTEDRYGRCVGDIYCDGIFVQKIMLKKGYAWHYTAYD 289

Query: 721 KRPDLDKWEKEARAKRIGLWASSNPEKPWEWRKNRR 828
           +RP+L+KWEKEARAKRIGLWASSNPE PWEWRK+RR
Sbjct: 290 RRPELEKWEKEARAKRIGLWASSNPEMPWEWRKDRR 325


>ref|XP_002311254.1| predicted protein [Populus trichocarpa] gi|222851074|gb|EEE88621.1|
           predicted protein [Populus trichocarpa]
          Length = 335

 Score =  389 bits (999), Expect = e-106
 Identities = 188/276 (68%), Positives = 213/276 (77%)
 Frame = +1

Query: 1   ANWYRKLSVTWRESNPPPITPEEAARLIIQTLKNHLKADVEGLLAYYGLPRPHSLVELSS 180
           ANWYRKL   W+E+ PPP TPEEAARL+IQTLK H KADVEGLLA+YGLP PH+L+++S+
Sbjct: 68  ANWYRKLLEAWKEAKPPPKTPEEAARLVIQTLKRHQKADVEGLLAFYGLPLPHTLIQVST 127

Query: 181 GEPQSRPEGLKFELHTLPVDVKAVADGDTVTVYVSTMDPRESXXXXXXXXXXXXXXXXXX 360
           G P S PEG+KFE+ TLPVD KAVADGDT+TVYVST DPRES                  
Sbjct: 128 GIPASLPEGVKFEMPTLPVDAKAVADGDTITVYVSTTDPRESAYVPREVQTATVQRSKAR 187

Query: 361 XXXXXXXXXXXXXXXXXEADALHKKITDMGYRVLNINNQEVLAKKYRIRLRGIDAPESKM 540
                            +ADALHKKI + GYRVLNI NQE+LA+KYRIRLRGIDAPES M
Sbjct: 188 AERNYG-----------KADALHKKIVEAGYRVLNIQNQEILARKYRIRLRGIDAPESAM 236

Query: 541 PYGPEAKEELVKIVQGKCLKVLVFTEDRYGRCVGDIYCNGIFVQESMLKKGLVWHYTAYD 720
           PYG EAKEEL  +VQGKCL++ V+ EDRYGRCVGDIYCNGIFVQE MLKKGL WHYTAYD
Sbjct: 237 PYGKEAKEELANLVQGKCLRIFVYGEDRYGRCVGDIYCNGIFVQEIMLKKGLAWHYTAYD 296

Query: 721 KRPDLDKWEKEARAKRIGLWASSNPEKPWEWRKNRR 828
           +R +L+ WEKEARAKR+GLWAS NPEKPWEWRK+RR
Sbjct: 297 QRWELETWEKEARAKRVGLWASPNPEKPWEWRKDRR 332


>gb|AAK52082.1| nuclease [Nicotiana tabacum]
          Length = 330

 Score =  383 bits (983), Expect = e-104
 Identities = 190/276 (68%), Positives = 211/276 (76%), Gaps = 1/276 (0%)
 Frame = +1

Query: 1   ANWYRKLSVTWRESNPPPITPEEAARLIIQTLKNHLKADVEGLLAYYGLPRPHSLVELSS 180
           A WY+KLS  WRE+ PPP TPEEA+RL+IQTLK H KADVEGLLA+YGLP PHSLVELSS
Sbjct: 60  AKWYKKLSDAWRETKPPPRTPEEASRLVIQTLKRHQKADVEGLLAFYGLPLPHSLVELSS 119

Query: 181 -GEPQSRPEGLKFELHTLPVDVKAVADGDTVTVYVSTMDPRESXXXXXXXXXXXXXXXXX 357
            G P S P+G+KFELHTLPVD +AVADGDTVTVYVST DPRES                 
Sbjct: 120 DGVPPSHPQGVKFELHTLPVDARAVADGDTVTVYVSTTDPRESSCLPRDVQVAAVQRSNA 179

Query: 358 XXXXXXXXXXXXXXXXXXEADALHKKITDMGYRVLNINNQEVLAKKYRIRLRGIDAPESK 537
                             +AD LHK+I D GYRV+ +NN+EVLA+KYRIRLRGIDAPES 
Sbjct: 180 RAQKNYP-----------KADELHKQIIDSGYRVIPVNNEEVLARKYRIRLRGIDAPESA 228

Query: 538 MPYGPEAKEELVKIVQGKCLKVLVFTEDRYGRCVGDIYCNGIFVQESMLKKGLVWHYTAY 717
           MPYG EA EEL K+VQGK L+VLV+ EDRYGR VGDIYCNGIFVQE MLKKGL WHYTAY
Sbjct: 229 MPYGKEATEELTKVVQGKSLRVLVYEEDRYGRSVGDIYCNGIFVQEVMLKKGLAWHYTAY 288

Query: 718 DKRPDLDKWEKEARAKRIGLWASSNPEKPWEWRKNR 825
           DKRP+L+KWEKEARAKRIGLWA+ NPE PWEWRK +
Sbjct: 289 DKRPELEKWEKEARAKRIGLWAARNPEMPWEWRKQK 324


>gb|AAX21543.1| Ca(2+)-dependent DNase [Populus tomentosa]
          Length = 335

 Score =  382 bits (982), Expect = e-104
 Identities = 186/276 (67%), Positives = 210/276 (76%)
 Frame = +1

Query: 1   ANWYRKLSVTWRESNPPPITPEEAARLIIQTLKNHLKADVEGLLAYYGLPRPHSLVELSS 180
           ANWYRKL   W+E+ PPP TPEEAARL+IQTLK H KADVEGLLA+YGLP  H+LV++S+
Sbjct: 68  ANWYRKLLEAWKEAKPPPKTPEEAARLVIQTLKRHQKADVEGLLAFYGLPLAHTLVQVST 127

Query: 181 GEPQSRPEGLKFELHTLPVDVKAVADGDTVTVYVSTMDPRESXXXXXXXXXXXXXXXXXX 360
           G P S PEG+KFE+ TLPVD KAVADGDT+TVYVST DPRES                  
Sbjct: 128 GIPTSLPEGVKFEMPTLPVDAKAVADGDTITVYVSTTDPRESAYVPREVQTATVQRSKAR 187

Query: 361 XXXXXXXXXXXXXXXXXEADALHKKITDMGYRVLNINNQEVLAKKYRIRLRGIDAPESKM 540
                            +ADALHKKI + GYRVLNI NQE+LA+KYRIRLRGIDAPES M
Sbjct: 188 AERNYG-----------KADALHKKIVEAGYRVLNIQNQEILARKYRIRLRGIDAPESAM 236

Query: 541 PYGPEAKEELVKIVQGKCLKVLVFTEDRYGRCVGDIYCNGIFVQESMLKKGLVWHYTAYD 720
           PYG EAKEE   +VQGKCL++ V+ EDRYGRCVGDIYCNGIFVQE MLKKGL WHYTAYD
Sbjct: 237 PYGKEAKEEFANLVQGKCLRIFVYGEDRYGRCVGDIYCNGIFVQEVMLKKGLAWHYTAYD 296

Query: 721 KRPDLDKWEKEARAKRIGLWASSNPEKPWEWRKNRR 828
           +R +L+ WEKEARAKR+GLWAS NPEKPWEWR +RR
Sbjct: 297 QRWELETWEKEARAKRVGLWASPNPEKPWEWRMDRR 332