BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002488
         (1391 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAI67717.1| protein disulfide isomerase 1 [Daucus carota]         510   e-142
gb|ABK96365.1| unknown [Populus trichocarpa x Populus deltoides]      452   e-125
ref|XP_002300980.1| predicted protein [Populus trichocarpa] gi|2...   451   e-124
ref|XP_002285864.1| PREDICTED: protein disulfide-isomerase [Viti...   446   e-123
sp|P29828.1|PDI_MEDSA RecName: Full=Protein disulfide-isomerase;...   437   e-120

>dbj|BAI67717.1| protein disulfide isomerase 1 [Daucus carota]
          Length = 515

 Score =  510 bits (1314), Expect = e-142
 Identities = 273/417 (65%), Positives = 299/417 (71%), Gaps = 2/417 (0%)
 Frame = +2

Query: 146  SRVSIGYYTVFIGLVLLSGSICSAEETS-ENVIEHVLTLDHSNFSETVEKHDFIVVEFYA 322
            SRV I  + V + L LLSGSICSAEE S E+V++HVLTLDHSNFSE V KH+ IVVEFYA
Sbjct: 5    SRVVISGFVVLMSLALLSGSICSAEEESKESVVDHVLTLDHSNFSEIVGKHESIVVEFYA 64

Query: 323  PWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGV-QGFPTIKILRN 499
            PWCGHCK+LAPEYEKAASVLSSHDP IVLAKVDANEE+NKE+A    V +  PT+KILRN
Sbjct: 65   PWCGHCKSLAPEYEKAASVLSSHDPAIVLAKVDANEEANKELAISISVFKVSPTLKILRN 124

Query: 500  GGKQQQEYKGPREADGIVSYLKKQVGPASTEIKXXXXXXXXXXXXXXXXXXXXXXXXXXX 679
            GGK  QEYKGPREA+GIVSYLKKQVGPAS EIK                           
Sbjct: 125  GGKLSQEYKGPREAEGIVSYLKKQVGPASAEIKSAEDASSLIDEKKIPLIGLFPVLS--- 181

Query: 680  XXXXXXXXXXXXXXXXXXXXXXXXGEEYDNFTALAEKLRSDYDFGHTLDAKLIPKGDSSI 859
                                    GEE++NFTA +EKLRSDYDFGHT+DAK IP+GDSSI
Sbjct: 182  ------------------------GEEFENFTAPSEKLRSDYDFGHTVDAKFIPQGDSSI 217

Query: 860  TKPTLRLLKPFDELFVDSQNFQVDAMEKFIDESSVPTVTLFNKDPSNHPFLVKFFDSPVA 1039
            +KPTLRLLKPFDELFVDSQ+F VDAME+FI ES VPTVTLFN+DPSNHPFLVKFFDSP A
Sbjct: 218  SKPTLRLLKPFDELFVDSQDFHVDAMEEFIAESGVPTVTLFNQDPSNHPFLVKFFDSPDA 277

Query: 1040 KVMLFLNFSTDKFDDFKTKYNDAAVXXXXXXXXXXXXXXEASQGAFQYFGLSEDQVPVLL 1219
            K MLFLNFSTDKFDDFK  YND AV              EAS+GAFQYFGLSEDQ PV+L
Sbjct: 278  KAMLFLNFSTDKFDDFKKNYNDVAVLYKGKGLNFLLGDLEASKGAFQYFGLSEDQAPVIL 337

Query: 1220 VQTSDGQKYLKANAEAGQIAPWLKDYVDGKLKPFVKSXXXXXXXXXXXXXXXRDSIQ 1390
            VQTSD QKYLK N EA QIAPWLK+Y+DGKLKP+VKS               RDSIQ
Sbjct: 338  VQTSDSQKYLKGNVEADQIAPWLKEYMDGKLKPYVKSDPIPEVNNEPVKVVVRDSIQ 394



 Score = 67.4 bits (163), Expect = 8e-09
 Identities = 34/88 (38%), Positives = 52/88 (59%)
 Frame = +2

Query: 305 VVEFYAPWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPTI 484
           ++EFYAPWCGHCK LAP  ++ A V   +D  +++AK DA   +N   +  + VQGFPT+
Sbjct: 405 LIEFYAPWCGHCKKLAPILDEVA-VSFENDADVIIAKFDAT--TNDVPSEVFDVQGFPTL 461

Query: 485 KILRNGGKQQQEYKGPREADGIVSYLKK 568
              R+       Y+G R  D  + +++K
Sbjct: 462 -YFRSASGTVVPYEGDRTKDDFIEFIQK 488


>gb|ABK96365.1| unknown [Populus trichocarpa x Populus deltoides]
          Length = 505

 Score =  452 bits (1164), Expect = e-125
 Identities = 231/397 (58%), Positives = 281/397 (70%)
 Frame = +2

Query: 140  MASRVSIGYYTVFIGLVLLSGSICSAEETSENVIEHVLTLDHSNFSETVEKHDFIVVEFY 319
            MAS VS       + L++   ++ + E+ S+   E+VLTLDHSNF+ETV KHDFIVVEFY
Sbjct: 1    MASTVSFWSCIFLLSLIV---ALTAGEDESK---EYVLTLDHSNFNETVSKHDFIVVEFY 54

Query: 320  APWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPTIKILRN 499
            APWCGHCK LAPEYEKAAS+LSS+DPQ+VLAKVDANE++NKEIASQY V+GFPTI ILR 
Sbjct: 55   APWCGHCKKLAPEYEKAASILSSNDPQVVLAKVDANEDANKEIASQYDVKGFPTIVILRK 114

Query: 500  GGKQQQEYKGPREADGIVSYLKKQVGPASTEIKXXXXXXXXXXXXXXXXXXXXXXXXXXX 679
            GGK  QEYKGPREADGIV YLKKQ GPAS E+K                           
Sbjct: 115  GGKSVQEYKGPREADGIVEYLKKQSGPASAELKSDDDATGFIGDKKVVIVGVFPKFS--- 171

Query: 680  XXXXXXXXXXXXXXXXXXXXXXXXGEEYDNFTALAEKLRSDYDFGHTLDAKLIPKGDSSI 859
                                    GEE++NF A+AEKLRSDY+FGHTLDAK +P+G+SS+
Sbjct: 172  ------------------------GEEFENFLAVAEKLRSDYEFGHTLDAKYLPRGESSV 207

Query: 860  TKPTLRLLKPFDELFVDSQNFQVDAMEKFIDESSVPTVTLFNKDPSNHPFLVKFFDSPVA 1039
            + P +RL KPFDELFVDS++F VDA+EKF++ESS+P VTLFNKDPSNHPF+VK+FDSP+A
Sbjct: 208  SGPLVRLFKPFDELFVDSKDFNVDALEKFVEESSIPIVTLFNKDPSNHPFVVKYFDSPLA 267

Query: 1040 KVMLFLNFSTDKFDDFKTKYNDAAVXXXXXXXXXXXXXXEASQGAFQYFGLSEDQVPVLL 1219
            K MLF+NFS++  D  +TKY + A               EASQGA QYFGL EDQVP+++
Sbjct: 268  KAMLFMNFSSENGDSIRTKYQEVAGLHKGDGLVFLLGDVEASQGALQYFGLKEDQVPLIV 327

Query: 1220 VQTSDGQKYLKANAEAGQIAPWLKDYVDGKLKPFVKS 1330
            +QT+DGQKYLKAN  + QIAPWLK+Y +GK+ PF KS
Sbjct: 328  IQTTDGQKYLKANLVSDQIAPWLKEYKEGKVPPFKKS 364



 Score = 69.3 bits (168), Expect = 2e-09
 Identities = 38/89 (42%), Positives = 53/89 (59%)
 Frame = +2

Query: 302 IVVEFYAPWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPT 481
           + +EFYAPWCGHC+ LAP  E+ A    S D  +V+AK+DA   +N   +  Y V+GFPT
Sbjct: 394 VFLEFYAPWCGHCQKLAPILEEVAISFQS-DADVVIAKLDAT--ANDIPSDTYDVKGFPT 450

Query: 482 IKILRNGGKQQQEYKGPREADGIVSYLKK 568
           I      GK  Q Y+G R    I+ +++K
Sbjct: 451 IFFRSATGKLVQ-YEGDRTKQDIIDFIEK 478


>ref|XP_002300980.1| predicted protein [Populus trichocarpa] gi|222842706|gb|EEE80253.1|
            predicted protein [Populus trichocarpa]
          Length = 505

 Score =  451 bits (1159), Expect = e-124
 Identities = 230/397 (57%), Positives = 280/397 (70%)
 Frame = +2

Query: 140  MASRVSIGYYTVFIGLVLLSGSICSAEETSENVIEHVLTLDHSNFSETVEKHDFIVVEFY 319
            MAS VS       + L++   ++ + E+ S+   E+VLTLDHSNF+ETV KHDFIVVEFY
Sbjct: 1    MASTVSFWSCIFLLSLIV---ALSAGEDESK---EYVLTLDHSNFNETVSKHDFIVVEFY 54

Query: 320  APWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPTIKILRN 499
            APWCGHCK LAPEYEKAAS+LSS+DPQ+VLAKVDANE++NKEIASQY V+GFPTI ILR 
Sbjct: 55   APWCGHCKKLAPEYEKAASILSSNDPQVVLAKVDANEDANKEIASQYDVKGFPTIVILRK 114

Query: 500  GGKQQQEYKGPREADGIVSYLKKQVGPASTEIKXXXXXXXXXXXXXXXXXXXXXXXXXXX 679
            GGK  QEYKGPREADGIV YLKKQ GPAS E+K                           
Sbjct: 115  GGKSVQEYKGPREADGIVEYLKKQSGPASAELKSDDDATGFIGDKKVVIVGVFPKFS--- 171

Query: 680  XXXXXXXXXXXXXXXXXXXXXXXXGEEYDNFTALAEKLRSDYDFGHTLDAKLIPKGDSSI 859
                                    GEE++NF A+AEKLRSDY+FGHTLDAK +P+G+SS+
Sbjct: 172  ------------------------GEEFENFLAVAEKLRSDYEFGHTLDAKYLPRGESSV 207

Query: 860  TKPTLRLLKPFDELFVDSQNFQVDAMEKFIDESSVPTVTLFNKDPSNHPFLVKFFDSPVA 1039
            + P +RL KPFDELFVDS++F VDA+EKF++ESS+P VTLFNKDPSNHPF+VK+FDSP+A
Sbjct: 208  SGPLVRLFKPFDELFVDSKDFNVDALEKFVEESSIPIVTLFNKDPSNHPFVVKYFDSPLA 267

Query: 1040 KVMLFLNFSTDKFDDFKTKYNDAAVXXXXXXXXXXXXXXEASQGAFQYFGLSEDQVPVLL 1219
            K MLF+NFS++  D  +TKY + A               EASQGA QYFGL EDQVP+++
Sbjct: 268  KAMLFMNFSSENGDSIRTKYQEVAGLHKGDGLVFLLGDVEASQGALQYFGLKEDQVPLIV 327

Query: 1220 VQTSDGQKYLKANAEAGQIAPWLKDYVDGKLKPFVKS 1330
            +QT+DGQKYLK N  + QIAPWLK+Y +GK+ PF KS
Sbjct: 328  IQTTDGQKYLKPNLVSDQIAPWLKEYKEGKVPPFKKS 364



 Score = 69.3 bits (168), Expect = 2e-09
 Identities = 38/89 (42%), Positives = 53/89 (59%)
 Frame = +2

Query: 302 IVVEFYAPWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPT 481
           + +EFYAPWCGHC+ LAP  E+ A    S D  +V+AK+DA   +N   +  Y V+GFPT
Sbjct: 394 VFLEFYAPWCGHCQKLAPILEEVAISFQS-DADVVIAKLDAT--ANDIPSDTYDVKGFPT 450

Query: 482 IKILRNGGKQQQEYKGPREADGIVSYLKK 568
           I      GK  Q Y+G R    I+ +++K
Sbjct: 451 IFFRSATGKLVQ-YEGDRTKQDIIDFIEK 478


>ref|XP_002285864.1| PREDICTED: protein disulfide-isomerase [Vitis vinifera]
            gi|302141800|emb|CBI19003.3| unnamed protein product
            [Vitis vinifera]
          Length = 499

 Score =  446 bits (1146), Expect = e-123
 Identities = 223/397 (56%), Positives = 274/397 (69%)
 Frame = +2

Query: 140  MASRVSIGYYTVFIGLVLLSGSICSAEETSENVIEHVLTLDHSNFSETVEKHDFIVVEFY 319
            MA+RVSI  + +     +L+ S        +   E VLTL HSNFS+ V KHDFIVVEFY
Sbjct: 1    MATRVSICLFVLAFAFSILASSPVKISAAEDEAKEFVLTLTHSNFSDIVSKHDFIVVEFY 60

Query: 320  APWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPTIKILRN 499
            APWCGHCK +APEYEKAAS+LSSHDP I+LAKVDAN+++NKE+AS++ ++GFPT+KILRN
Sbjct: 61   APWCGHCKKIAPEYEKAASILSSHDPPIILAKVDANDDANKELASEFEIRGFPTLKILRN 120

Query: 500  GGKQQQEYKGPREADGIVSYLKKQVGPASTEIKXXXXXXXXXXXXXXXXXXXXXXXXXXX 679
            GGK  +EYKGPREADGIV YLKKQ GPAS EIK                           
Sbjct: 121  GGKSIEEYKGPREADGIVEYLKKQSGPASAEIKSAEDASSLIVDNKIVIVGVFPKFS--- 177

Query: 680  XXXXXXXXXXXXXXXXXXXXXXXXGEEYDNFTALAEKLRSDYDFGHTLDAKLIPKGDSSI 859
                                    GEE++NFTA+AEK RSDYDF HT DAK +P+G+SS+
Sbjct: 178  ------------------------GEEFENFTAVAEKFRSDYDFVHTSDAKFLPRGESSV 213

Query: 860  TKPTLRLLKPFDELFVDSQNFQVDAMEKFIDESSVPTVTLFNKDPSNHPFLVKFFDSPVA 1039
            T P +RLLKPFDELFVD Q+F VDA+EKF++ESSVP VTLFNKDPSNHPF++KFF+ P A
Sbjct: 214  TGPLVRLLKPFDELFVDFQDFHVDALEKFVEESSVPIVTLFNKDPSNHPFVIKFFNGPNA 273

Query: 1040 KVMLFLNFSTDKFDDFKTKYNDAAVXXXXXXXXXXXXXXEASQGAFQYFGLSEDQVPVLL 1219
            K MLFL+ S++ FD FK+KY++ A               EASQGAFQYFGL +DQVP+++
Sbjct: 274  KAMLFLDHSSELFDAFKSKYHEVAEQYKGKGINFLLGDLEASQGAFQYFGLKDDQVPLIV 333

Query: 1220 VQTSDGQKYLKANAEAGQIAPWLKDYVDGKLKPFVKS 1330
            +QT+DGQKYLK N E   IAPW+K+Y DGK+ P+ KS
Sbjct: 334  IQTNDGQKYLKPNLEPDHIAPWVKEYQDGKVLPYKKS 370



 Score = 69.7 bits (169), Expect = 2e-09
 Identities = 36/97 (37%), Positives = 57/97 (58%)
 Frame = +2

Query: 302 IVVEFYAPWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPT 481
           ++VEFYAPWCGHCK LAP  ++ A +   +D  +V+AK+DA   +N      + V+G+PT
Sbjct: 400 VLVEFYAPWCGHCKKLAPILDEVA-ISFENDADVVIAKLDAT--ANDIPNDTFDVKGYPT 456

Query: 482 IKILRNGGKQQQEYKGPREADGIVSYLKKQVGPASTE 592
           +      G   Q Y+G R  + I+ ++KK    A+ +
Sbjct: 457 LYFKSASGNISQ-YEGDRSKEDIIEFIKKNRDKAAQQ 492


>sp|P29828.1|PDI_MEDSA RecName: Full=Protein disulfide-isomerase; Short=PDI; Flags:
            Precursor gi|19654|emb|CAA77575.1| protein disulfide
            isomerase [Medicago sativa]
          Length = 512

 Score =  437 bits (1125), Expect = e-120
 Identities = 225/397 (56%), Positives = 276/397 (69%)
 Frame = +2

Query: 140  MASRVSIGYYTVFIGLVLLSGSICSAEETSENVIEHVLTLDHSNFSETVEKHDFIVVEFY 319
            MA  V+I  + +   L+LL  S   AEE+S +  E VLTLD++NF +TV+KHDFIVVEFY
Sbjct: 1    MAKNVAI--FGLLFSLLLLVPSQIFAEESSTDAKEFVLTLDNTNFHDTVKKHDFIVVEFY 58

Query: 320  APWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPTIKILRN 499
            APWCGHCK LAPEYEKAAS+LS+H+P +VLAKVDANEE NK++AS+  V+GFPTIKI RN
Sbjct: 59   APWCGHCKKLAPEYEKAASILSTHEPPVVLAKVDANEEHNKDLASENDVKGFPTIKIFRN 118

Query: 500  GGKQQQEYKGPREADGIVSYLKKQVGPASTEIKXXXXXXXXXXXXXXXXXXXXXXXXXXX 679
            GGK  QEYKGPREA+GIV YLKKQ GPASTEIK                           
Sbjct: 119  GGKNIQEYKGPREAEGIVEYLKKQSGPASTEIKSADDATAFVGDNKVVIVGVFPKFS--- 175

Query: 680  XXXXXXXXXXXXXXXXXXXXXXXXGEEYDNFTALAEKLRSDYDFGHTLDAKLIPKGDSSI 859
                                    GEEYDNF ALAEKLRSDYDF HTL+AK +PKGDSS+
Sbjct: 176  ------------------------GEEYDNFIALAEKLRSDYDFAHTLNAKHLPKGDSSV 211

Query: 860  TKPTLRLLKPFDELFVDSQNFQVDAMEKFIDESSVPTVTLFNKDPSNHPFLVKFFDSPVA 1039
            + P +RL KPFDELFVDS++F V+A+EKFI+ESS P VT+FN +PSNHPF+VKFF+SP A
Sbjct: 212  SGPVVRLFKPFDELFVDSKDFNVEALEKFIEESSTPIVTVFNNEPSNHPFVVKFFNSPNA 271

Query: 1040 KVMLFLNFSTDKFDDFKTKYNDAAVXXXXXXXXXXXXXXEASQGAFQYFGLSEDQVPVLL 1219
            K MLF+NF+T+  + FKTKY++ A               E+SQGAFQYFGL E+QVP+++
Sbjct: 272  KAMLFINFTTEGAESFKTKYHEVAEQYKQQGVSFLVGDVESSQGAFQYFGLKEEQVPLII 331

Query: 1220 VQTSDGQKYLKANAEAGQIAPWLKDYVDGKLKPFVKS 1330
            +Q +DG+K+ K N E  Q+  WLK Y DGK++PFVKS
Sbjct: 332  IQHNDGKKFFKPNLELDQLPTWLKAYKDGKVEPFVKS 368



 Score = 71.6 bits (174), Expect = 4e-10
 Identities = 39/102 (38%), Positives = 59/102 (57%), Gaps = 3/102 (2%)
 Frame = +2

Query: 302 IVVEFYAPWCGHCKNLAPEYEKAASVLSSHDPQIVLAKVDANEESNKEIASQYGVQGFPT 481
           +++EFYAPWCGHCK LAP  ++ A    S D  +V+AK+DA   +N      + VQG+PT
Sbjct: 398 VLIEFYAPWCGHCKQLAPILDEVAVSFQS-DADVVIAKLDAT--ANDIPTDTFDVQGYPT 454

Query: 482 IKILRNGGKQQQEYKGPREADGIVSYLKK---QVGPASTEIK 598
           +      GK  Q Y G R  + I+ +++K   + G A  E++
Sbjct: 455 LYFRSASGKLSQ-YDGGRTKEDIIEFIEKNKDKTGAAHQEVE 495