BLASTX nr result
ID: Bupleurum21_contig00002472
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00002472
(1581 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EME31274.1| importin alpha [Galdieria sulphuraria] 77 1e-11
gb|ABM05487.1| Impa1 [Nicotiana benthamiana] 77 1e-11
ref|NP_001234006.1| importin subunit alpha [Solanum lycopersicum... 77 2e-11
dbj|BAE71209.1| putative importin alpha [Trifolium pratense] 74 1e-10
ref|XP_002281670.1| PREDICTED: importin subunit alpha-1 [Vitis v... 73 2e-10
>gb|EME31274.1| importin alpha [Galdieria sulphuraria]
Length = 531
Score = 77.4 bits (189), Expect = 1e-11
Identities = 82/313 (26%), Positives = 129/313 (41%), Gaps = 5/313 (1%)
Frame = +1
Query: 652 IDNLTATMKNAASMNQLEDIEKCTRELYYI--IKMYPKVCTYLKKDDFKLLVGFLKNELY 825
I L + +A + QLE CT+E + I+ P + + V FL E
Sbjct: 76 IPKLVQDIWSADTATQLE----CTKEFRQLLSIEKNPPISEVISTSVVPRFVEFLNREDS 131
Query: 826 PNLQSLVAIILQSVFANS-RNGNMVIEYITSELLVKLISSNHGDVHVQALELLHSITMAN 1002
P LQ A +L ++ + + + V+E + VKL++S H DV QA+ L +I A
Sbjct: 132 PQLQFEAAWVLTNIASGTCEHTTTVVEAGAVPIFVKLMTSPHEDVREQAIWALGNI--AG 189
Query: 1003 PGPEFKANLEEALGPLTVLL--FSKLTDDQVLHSSSKALAAICLVYPELPPDKLKTVLNG 1176
PE++ NL G + LL S + +L +++ L+ +C P+ P D ++ L
Sbjct: 190 DSPEYR-NLVLQQGAMAPLLEQLSHTSKFSMLRNATWTLSNLCRGKPQPPFDLVRPALKT 248
Query: 1177 LVNILKHKSEEFMNDACSGFHYHWDGSYRKILLNACLGFQYLCDGRKNMFLEKEYFEPLY 1356
L +++ EE + DAC Y DGS KI +E L
Sbjct: 249 LAHLVYSNDEEVLTDACWALSYLSDGSNDKI----------------QAVIESGVTRRLV 292
Query: 1357 EAXXXXXXXWDRNGSHEIPVAALNALGSIVRCSDRFIEFMIDRDQLSLIVDALEQEELHF 1536
E + S + AL +G+IV D + +I+ L + L +
Sbjct: 293 ELLM--------HQSVSVQTPALRTIGNIVTGDDTQTQIVINCSALPCLATLLNSPKKSI 344
Query: 1537 VKIVCWIFSNITA 1575
K CW SNITA
Sbjct: 345 RKEACWTISNITA 357
>gb|ABM05487.1| Impa1 [Nicotiana benthamiana]
Length = 532
Score = 77.4 bits (189), Expect = 1e-11
Identities = 91/370 (24%), Positives = 155/370 (41%), Gaps = 8/370 (2%)
Frame = +1
Query: 490 IDRNSGKKRNREKQIKIY*RMKRDRDEYLKDPLFTRVIWKDPFFEFVYETKK--KEIDNL 663
+D G++R + ++I K R+E L ++ F + K+++NL
Sbjct: 20 VDAEEGRRRREDNMVEI---RKNKREESLLKKRREGLLQAQQFSSTAAAVSQLDKKLENL 76
Query: 664 TATMKNAASMNQLEDIEKCTRELYYI--IKMYPKVCTYLKKDDFKLLVGFLKNELYPNLQ 837
+ S + +E CT + + I+ P + ++ V FL E YP LQ
Sbjct: 77 PDMISGVWSDDSSMQLE-CTTQFRKLLSIERNPPIEEVIQSGVVPRFVEFLAREDYPQLQ 135
Query: 838 SLVAIILQSVFAN-SRNGNMVIEYITSELLVKLISSNHGDVHVQALELLHSITMANPGPE 1014
A L ++ + S N +VI+Y + + V+L+SS DV QA+ L +I A P+
Sbjct: 136 FEAAWALTNIASGTSENTKVVIDYGSVPIFVRLLSSPSDDVREQAVWALGNI--AGDSPK 193
Query: 1015 FKANLEEALGPLTVLL--FSKLTDDQVLHSSSKALAAICLVYPELPPDKLKTVLNGLVNI 1188
++ +L G L LL F++ +L +++ L+ C P+ ++ K L L +
Sbjct: 194 YR-DLVLGHGALAPLLAQFNEHAKLSMLRNATWTLSNFCRGKPQPQFEQTKEALPTLARL 252
Query: 1189 LKHKSEEFMNDACSGFHYHWDGSYRKILLNACLGFQYLCDGRKNMFLEKEYFEPLYEAXX 1368
+ EE + DAC Y DG+ KI G +C + L
Sbjct: 253 IHSNDEEVLTDACWALSYLSDGTNDKIQAVIEAG---VCPRLIELLL------------- 296
Query: 1369 XXXXXWDRNGSHEIPVAALNALGSIVRCSDRFIEFMIDRDQLSLIVDALEQEELHFVKI- 1545
+ S + + AL +G+IV D + +ID L +++ L Q +K
Sbjct: 297 --------HSSPSVLIPALRTVGNIVTGDDIQTQVIIDHHALPCLLNLLTQNYKKSIKKE 348
Query: 1546 VCWIFSNITA 1575
CW SNITA
Sbjct: 349 ACWTISNITA 358
>ref|NP_001234006.1| importin subunit alpha [Solanum lycopersicum]
gi|3915737|sp|O22478.2|IMA_SOLLC RecName: Full=Importin
subunit alpha; AltName: Full=Karyopherin subunit alpha;
Short=KAP alpha gi|3228370|gb|AAC23722.1| importin alpha
[Solanum lycopersicum]
Length = 527
Score = 76.6 bits (187), Expect = 2e-11
Identities = 93/372 (25%), Positives = 155/372 (41%), Gaps = 10/372 (2%)
Frame = +1
Query: 490 IDRNSGKKRNREKQIKIY*RMKRDRDEYLKDPLFTRVIWKDPFFEFV----YETKKKEID 657
+D G++R + ++I K R+E L ++ F + K + +
Sbjct: 20 VDAEEGRRRREDNMVEI---RKNKREENLLKKRREGLLQAQQFPSTAAVSHLDKKLETLP 76
Query: 658 NLTATMKNAASMNQLEDIEKCTRELYYI--IKMYPKVCTYLKKDDFKLLVGFLKNELYPN 831
L A + + S QLE CT + + I+ P + ++ V FL + YP
Sbjct: 77 ELIAGVWSDDSSLQLE----CTTQFRKLLSIERNPPIEEVIQSGVVPRFVEFLARDDYPQ 132
Query: 832 LQSLVAIILQSVFAN-SRNGNMVIEYITSELLVKLISSNHGDVHVQALELLHSITMANPG 1008
LQ A L ++ + S N +VI+Y + + ++L+SS DV QA+ L +I A
Sbjct: 133 LQFEAAWALTNIASGTSENTKVVIDYGSVPIFIRLLSSPSDDVREQAVWALGNI--AGDS 190
Query: 1009 PEFKANLEEALGPLTVLL--FSKLTDDQVLHSSSKALAAICLVYPELPPDKLKTVLNGLV 1182
P+++ +L G L LL F++ +L +++ L+ C P+ ++ K L L
Sbjct: 191 PKYR-DLVLGHGALVALLAQFNEQAKLSMLRNATWTLSNFCRGKPQPLFEQTKAALPTLG 249
Query: 1183 NILKHKSEEFMNDACSGFHYHWDGSYRKILLNACLGFQYLCDGRKNMFLEKEYFEPLYEA 1362
++ EE + DAC Y DG+ KI G +C + L
Sbjct: 250 RLIHSNDEEVLTDACWALSYLSDGTNDKIQAVIEAG---VCSRLVELLL----------- 295
Query: 1363 XXXXXXXWDRNGSHEIPVAALNALGSIVRCSDRFIEFMIDRDQLSLIVDALEQEELHFVK 1542
+ S + + AL +G+IV D + MID L +V+ L Q +K
Sbjct: 296 ----------HSSPSVLIPALRTVGNIVTGDDIQTQVMIDHHALPCLVNLLTQNYKKSIK 345
Query: 1543 I-VCWIFSNITA 1575
CW SNITA
Sbjct: 346 KEACWTISNITA 357
>dbj|BAE71209.1| putative importin alpha [Trifolium pratense]
Length = 533
Score = 73.6 bits (179), Expect = 1e-10
Identities = 87/367 (23%), Positives = 153/367 (41%), Gaps = 5/367 (1%)
Frame = +1
Query: 490 IDRNSGKKRNREKQIKIY*RMKRDRDEYLKDPLFTRVIWKDPFFEFVYETKKKEIDNLTA 669
+D G++R + ++I K R+E L + + +K+++NL +
Sbjct: 22 VDAEEGRRRREDTMVEI---RKNRREESLMKKRREGLPPQQIPSSLHSTVVEKKLENLPS 78
Query: 670 TMKNAASMNQLEDIEKCTRELYYI-IKMYPKVCTYLKKDDFKLLVGFLKNELYPNLQSLV 846
+ +A S + +E T+ + I+ P + ++ V FL E +P LQ
Sbjct: 79 MVASAWSDDNNLQLEATTQFRKLLSIERTPPIEEVIQTGVVSRFVEFLMREDFPQLQFEA 138
Query: 847 AIILQSVFAN-SRNGNMVIEYITSELLVKLISSNHGDVHVQALELLHSITMANPGPEFKA 1023
A L ++ + S N +VI++ + VKL++S+ DV QA+ L ++ +P
Sbjct: 139 AWALTNIASGTSENTKVVIDHGAVPIFVKLLASSSDDVREQAVWALGNVAGDSPRCRDLV 198
Query: 1024 NLEEALGPLTVLL--FSKLTDDQVLHSSSKALAAICLVYPELPPDKLKTVLNGLVNILKH 1197
AL PL L +KL+ +L +++ L+ C P+ P D++K L L ++
Sbjct: 199 LGHGALVPLLAQLNEHAKLS---MLRNATWTLSNFCRGKPQPPFDQVKPALPALAGLIHS 255
Query: 1198 KSEEFMNDACSGFHYHWDGSYRKILLNACLGFQYLCDGRKNMFLEKEYFEPLYEAXXXXX 1377
EE + DAC Y DG+ KI G +C + L
Sbjct: 256 NDEEVLTDACWALSYLSDGTNDKIQAVIEAG---VCPRLVELLLHP-------------- 298
Query: 1378 XXWDRNGSHEIPVAALNALGSIVRCSDRFIEFMIDRDQLSLIVDALEQEELHFVKI-VCW 1554
S + + AL +G+IV D+ + +I+ L + + L +K CW
Sbjct: 299 -------SPSVLIPALRTVGNIVTGDDQQTQVIINHQSLPCLFNLLTNNYKKSIKKEACW 351
Query: 1555 IFSNITA 1575
SNITA
Sbjct: 352 TISNITA 358
>ref|XP_002281670.1| PREDICTED: importin subunit alpha-1 [Vitis vinifera]
gi|296089795|emb|CBI39614.3| unnamed protein product
[Vitis vinifera]
Length = 535
Score = 72.8 bits (177), Expect = 2e-10
Identities = 91/376 (24%), Positives = 150/376 (39%), Gaps = 8/376 (2%)
Frame = +1
Query: 472 KTMLAEIDRNSGKKRNREKQIKIY*RMKRDRDEYLKDPLFTRVIWKDPFFEFVYETK--- 642
K+ +D ++R + ++I R + D LK ++ F +T
Sbjct: 14 KSYKTGVDAEEARRRREDNLVEI--RKNKREDNLLKKRREGLILQSQQFSSDASQTTAVV 71
Query: 643 KKEIDNLTATMKNAASMNQLEDIEKCTRELYYI-IKMYPKVCTYLKKDDFKLLVGFLKNE 819
+K ++++T ++ S + +E T+ + I+ P + +K V FL
Sbjct: 72 EKRLESITLMVQGVWSDDPASQLEATTQFRKLLSIERSPPIDEVIKAGVVPRFVEFLGRH 131
Query: 820 LYPNLQSLVAIILQSVFAN-SRNGNMVIEYITSELLVKLISSNHGDVHVQALELLHSITM 996
P LQ A L +V + S + +VIE+ + V+L+SS DV QA+ L ++
Sbjct: 132 DLPQLQFEAAWALTNVASGTSEHTRVVIEHGAVPMFVQLLSSASDDVREQAVWALGNVAG 191
Query: 997 ANPGPEFKANLEEALGPLTVLL--FSKLTDDQVLHSSSKALAAICLVYPELPPDKLKTVL 1170
+P AL PL L SKL+ +L +++ L+ C P P +++K L
Sbjct: 192 DSPSCRDLVLGHGALMPLLSQLNEHSKLS---MLRNATWTLSNFCRGKPPTPFEQVKPAL 248
Query: 1171 NGLVNILKHKSEEFMNDACSGFHYHWDGSYRKILLNACLGFQYLCDGRKNMFLEKEYFEP 1350
L ++ EE + DAC Y DG KI G +C + L
Sbjct: 249 PVLRQLIHLNDEEVLTDACWALSYLSDGPNDKIQAVLEAG---VCPRLVELLLHP----- 300
Query: 1351 LYEAXXXXXXXWDRNGSHEIPVAALNALGSIVRCSDRFIEFMIDRDQLSLIVDALEQEEL 1530
S + + AL +G+IV D +F+ID L + L Q
Sbjct: 301 ----------------SPTVLIPALRTVGNIVTGDDAQTQFVIDNQVLPCLYQLLTQNHK 344
Query: 1531 HFVKI-VCWIFSNITA 1575
+K CW SNITA
Sbjct: 345 KSIKKEACWTISNITA 360