BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002458
         (2114 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265294.2| PREDICTED: alanine aminotransferase 2 [Vitis...   866   0.0  
ref|XP_002304255.1| predicted protein [Populus trichocarpa] gi|2...   863   0.0  
emb|CBI35796.3| unnamed protein product [Vitis vinifera]              863   0.0  
gb|AET06148.1| PLP-dependent aminotransferase [Papaver somniferum]    858   0.0  
gb|AAR05449.1| alanine aminotransferase [Capsicum annuum]             849   0.0  

>ref|XP_002265294.2| PREDICTED: alanine aminotransferase 2 [Vitis vinifera]
          Length = 526

 Score =  866 bits (2238), Expect = 0.0
 Identities = 426/494 (86%), Positives = 459/494 (92%)
 Frame = -1

Query: 1805 NQSPPPNLSAMPPSYSITLDSINPKVLECEYAVRGEIVILAQKLQQELLDNPGSHPFDEI 1626
            + SP  + SA  P+  +TL++INPKVLECEYAVRGEIV LAQ+LQQEL + PGS PFDEI
Sbjct: 34   SDSPSSSSSAAMPT-PVTLETINPKVLECEYAVRGEIVTLAQRLQQELQEKPGSLPFDEI 92

Query: 1625 LYCNIGNPQSLNQQPITFFREVLALCDHPAILDKSETQGLFSADAIERAWQILDQIPGRA 1446
            LYCNIGNPQSL QQPITFFREV+ALCDHP ILDKSETQGLFSADAIERAWQILDQIPGRA
Sbjct: 93   LYCNIGNPQSLGQQPITFFREVIALCDHPPILDKSETQGLFSADAIERAWQILDQIPGRA 152

Query: 1445 TGAYSHSQGIKGLRDTIAAGIEARDGFPADSNDLFLTDGASPAVHMMMQLLISSKKDGIL 1266
            TGAYSHSQGIKGLRDTIAAGIEARDGFP++ ND+FLTDGASPAVHMMMQLLI ++KDGIL
Sbjct: 153  TGAYSHSQGIKGLRDTIAAGIEARDGFPSNPNDIFLTDGASPAVHMMMQLLIRTEKDGIL 212

Query: 1265 IPIPQYPLYSASIALHGGTLVPYYLDEATGWGLEISELKKQLEDAKSNGITVKALVVINP 1086
             PIPQYPLYSASI LHGGTLVPYYLDEATGWGLEISEL+KQLEDAKS GITV+ALVVINP
Sbjct: 213  CPIPQYPLYSASIVLHGGTLVPYYLDEATGWGLEISELQKQLEDAKSKGITVRALVVINP 272

Query: 1085 GNPTGQVLAEENQRDIVKFCKAEGLVLLADEVYQENVYVPEKKFHSFKKVARSMGYGEKD 906
            GNPTGQVL+E+NQRDIV+FCK EGLVLLADEVYQENVYVP+KKFHSFKK++RSMGYGE+D
Sbjct: 273  GNPTGQVLSEDNQRDIVEFCKKEGLVLLADEVYQENVYVPDKKFHSFKKISRSMGYGEED 332

Query: 905  IPLVSFQSVSKGYHGECGKRGGYMEVTGFSPEIREQIYKVASVNLCSNISGQILASLVMC 726
            I +VSFQSVSKGY+GECGKRGGYMEVTGF  ++REQIYKVASVNLCSNI+GQILASLVM 
Sbjct: 333  ISVVSFQSVSKGYYGECGKRGGYMEVTGFGADVREQIYKVASVNLCSNITGQILASLVMS 392

Query: 725  PPKVGDESYESYTAEKDGILSSLARRAKTLEDAFNGLEGITCNKAEGAMYLFPRLHLPQN 546
            PPKVGDESYESY  EKDGILSSLARRAKTLEDA N LEG+TCNKAEGAMYLFPR+HLP+ 
Sbjct: 393  PPKVGDESYESYFMEKDGILSSLARRAKTLEDALNSLEGVTCNKAEGAMYLFPRIHLPEK 452

Query: 545  AIKAAETAKTAPDAFYARRLLNATGIVVVPGSGFRQVPGTWHIRCTILPPEDKIPAIVGR 366
            AIKAAE AKTAPDAFYARRLLNATGIVVVPGSGF QVPGTWH RCTILP EDKIPA+V R
Sbjct: 453  AIKAAEAAKTAPDAFYARRLLNATGIVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSR 512

Query: 365  LTEFHKSFMDEFRD 324
            LTEFHK FM+EFRD
Sbjct: 513  LTEFHKGFMEEFRD 526


>ref|XP_002304255.1| predicted protein [Populus trichocarpa] gi|222841687|gb|EEE79234.1|
            predicted protein [Populus trichocarpa]
          Length = 481

 Score =  863 bits (2230), Expect = 0.0
 Identities = 429/484 (88%), Positives = 449/484 (92%)
 Frame = -1

Query: 1775 MPPSYSITLDSINPKVLECEYAVRGEIVILAQKLQQELLDNPGSHPFDEILYCNIGNPQS 1596
            MPP   ++LD+INPKVL+CEYAVRGEIV LAQ LQ+EL   PGSHPFDEILYCNIGNPQS
Sbjct: 1    MPP---VSLDNINPKVLKCEYAVRGEIVTLAQALQEELKSKPGSHPFDEILYCNIGNPQS 57

Query: 1595 LNQQPITFFREVLALCDHPAILDKSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGI 1416
            L QQPITFFREVLALCDHP+ILD+SETQGLFSADAIERAWQILDQIPGRATGAYSHSQGI
Sbjct: 58   LGQQPITFFREVLALCDHPSILDRSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGI 117

Query: 1415 KGLRDTIAAGIEARDGFPADSNDLFLTDGASPAVHMMMQLLISSKKDGILIPIPQYPLYS 1236
            KGLRDTIAAGIEARDGFPAD ND+FLTDGASPAVHMMMQLLI S+KDGIL PIPQYPLYS
Sbjct: 118  KGLRDTIAAGIEARDGFPADPNDIFLTDGASPAVHMMMQLLIRSEKDGILCPIPQYPLYS 177

Query: 1235 ASIALHGGTLVPYYLDEATGWGLEISELKKQLEDAKSNGITVKALVVINPGNPTGQVLAE 1056
            ASIALHGG LVPYYLDEATGWGLE+SELKKQL DAKS GIT +ALVVINPGNPTGQVLAE
Sbjct: 178  ASIALHGGALVPYYLDEATGWGLEVSELKKQLADAKSKGITPRALVVINPGNPTGQVLAE 237

Query: 1055 ENQRDIVKFCKAEGLVLLADEVYQENVYVPEKKFHSFKKVARSMGYGEKDIPLVSFQSVS 876
            +NQR IV+FCK EGLVLLADEVYQENVYVPEKKF SFKKVARSMGYGEKDI LVSFQSVS
Sbjct: 238  DNQRGIVEFCKQEGLVLLADEVYQENVYVPEKKFDSFKKVARSMGYGEKDISLVSFQSVS 297

Query: 875  KGYHGECGKRGGYMEVTGFSPEIREQIYKVASVNLCSNISGQILASLVMCPPKVGDESYE 696
            KGY+GECGKRGGYMEVTGF PEIREQIYKVASVNLCSNISGQILASLVM PPKVGDESYE
Sbjct: 298  KGYYGECGKRGGYMEVTGFGPEIREQIYKVASVNLCSNISGQILASLVMSPPKVGDESYE 357

Query: 695  SYTAEKDGILSSLARRAKTLEDAFNGLEGITCNKAEGAMYLFPRLHLPQNAIKAAETAKT 516
            SY+AEKDGILSSLARRAKTLEDAFN LE +TCNKAEGAMYLFP + LP+ AIKAAE AKT
Sbjct: 358  SYSAEKDGILSSLARRAKTLEDAFNNLEAVTCNKAEGAMYLFPCICLPEKAIKAAEAAKT 417

Query: 515  APDAFYARRLLNATGIVVVPGSGFRQVPGTWHIRCTILPPEDKIPAIVGRLTEFHKSFMD 336
            APD FY RRLLNATGIV VPGSGF Q+PGTWH RCTILP EDKIPA+V RLTEFHKSFMD
Sbjct: 418  APDNFYCRRLLNATGIVFVPGSGFGQLPGTWHFRCTILPQEDKIPAVVSRLTEFHKSFMD 477

Query: 335  EFRD 324
            EFRD
Sbjct: 478  EFRD 481


>emb|CBI35796.3| unnamed protein product [Vitis vinifera]
          Length = 482

 Score =  863 bits (2229), Expect = 0.0
 Identities = 421/478 (88%), Positives = 451/478 (94%)
 Frame = -1

Query: 1757 ITLDSINPKVLECEYAVRGEIVILAQKLQQELLDNPGSHPFDEILYCNIGNPQSLNQQPI 1578
            +TL++INPKVLECEYAVRGEIV LAQ+LQQEL + PGS PFDEILYCNIGNPQSL QQPI
Sbjct: 5    VTLETINPKVLECEYAVRGEIVTLAQRLQQELQEKPGSLPFDEILYCNIGNPQSLGQQPI 64

Query: 1577 TFFREVLALCDHPAILDKSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGIKGLRDT 1398
            TFFREV+ALCDHP ILDKSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGIKGLRDT
Sbjct: 65   TFFREVIALCDHPPILDKSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGIKGLRDT 124

Query: 1397 IAAGIEARDGFPADSNDLFLTDGASPAVHMMMQLLISSKKDGILIPIPQYPLYSASIALH 1218
            IAAGIEARDGFP++ ND+FLTDGASPAVHMMMQLLI ++KDGIL PIPQYPLYSASI LH
Sbjct: 125  IAAGIEARDGFPSNPNDIFLTDGASPAVHMMMQLLIRTEKDGILCPIPQYPLYSASIVLH 184

Query: 1217 GGTLVPYYLDEATGWGLEISELKKQLEDAKSNGITVKALVVINPGNPTGQVLAEENQRDI 1038
            GGTLVPYYLDEATGWGLEISEL+KQLEDAKS GITV+ALVVINPGNPTGQVL+E+NQRDI
Sbjct: 185  GGTLVPYYLDEATGWGLEISELQKQLEDAKSKGITVRALVVINPGNPTGQVLSEDNQRDI 244

Query: 1037 VKFCKAEGLVLLADEVYQENVYVPEKKFHSFKKVARSMGYGEKDIPLVSFQSVSKGYHGE 858
            V+FCK EGLVLLADEVYQENVYVP+KKFHSFKK++RSMGYGE+DI +VSFQSVSKGY+GE
Sbjct: 245  VEFCKKEGLVLLADEVYQENVYVPDKKFHSFKKISRSMGYGEEDISVVSFQSVSKGYYGE 304

Query: 857  CGKRGGYMEVTGFSPEIREQIYKVASVNLCSNISGQILASLVMCPPKVGDESYESYTAEK 678
            CGKRGGYMEVTGF  ++REQIYKVASVNLCSNI+GQILASLVM PPKVGDESYESY  EK
Sbjct: 305  CGKRGGYMEVTGFGADVREQIYKVASVNLCSNITGQILASLVMSPPKVGDESYESYFMEK 364

Query: 677  DGILSSLARRAKTLEDAFNGLEGITCNKAEGAMYLFPRLHLPQNAIKAAETAKTAPDAFY 498
            DGILSSLARRAKTLEDA N LEG+TCNKAEGAMYLFPR+HLP+ AIKAAE AKTAPDAFY
Sbjct: 365  DGILSSLARRAKTLEDALNSLEGVTCNKAEGAMYLFPRIHLPEKAIKAAEAAKTAPDAFY 424

Query: 497  ARRLLNATGIVVVPGSGFRQVPGTWHIRCTILPPEDKIPAIVGRLTEFHKSFMDEFRD 324
            ARRLLNATGIVVVPGSGF QVPGTWH RCTILP EDKIPA+V RLTEFHK FM+EFRD
Sbjct: 425  ARRLLNATGIVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSRLTEFHKGFMEEFRD 482


>gb|AET06148.1| PLP-dependent aminotransferase [Papaver somniferum]
          Length = 484

 Score =  858 bits (2217), Expect = 0.0
 Identities = 420/479 (87%), Positives = 451/479 (94%)
 Frame = -1

Query: 1760 SITLDSINPKVLECEYAVRGEIVILAQKLQQELLDNPGSHPFDEILYCNIGNPQSLNQQP 1581
            S+T+DSINPKVL+CEYAVRG IV  AQKLQ+E+ +NPGSHPFDEILYCNIGNPQSL QQP
Sbjct: 6    SVTIDSINPKVLQCEYAVRGAIVNQAQKLQEEIKENPGSHPFDEILYCNIGNPQSLGQQP 65

Query: 1580 ITFFREVLALCDHPAILDKSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGIKGLRD 1401
            ITFFREVLALCDHP ILDKSETQGLFSAD+IERAWQILDQ+PGRATGAYSHSQG+K LRD
Sbjct: 66   ITFFREVLALCDHPVILDKSETQGLFSADSIERAWQILDQMPGRATGAYSHSQGVKCLRD 125

Query: 1400 TIAAGIEARDGFPADSNDLFLTDGASPAVHMMMQLLISSKKDGILIPIPQYPLYSASIAL 1221
             +AAGI ARDGFPAD ND+FLTDGASPAVHMMMQLLISS+KDG+L PIPQYPLYSASIAL
Sbjct: 126  AVAAGIAARDGFPADPNDIFLTDGASPAVHMMMQLLISSEKDGVLCPIPQYPLYSASIAL 185

Query: 1220 HGGTLVPYYLDEATGWGLEISELKKQLEDAKSNGITVKALVVINPGNPTGQVLAEENQRD 1041
            HGG+LVPYYLDEATGWGLE+SELKKQLEDAKS GITV++LVVINPGNPTGQVLAEENQR 
Sbjct: 186  HGGSLVPYYLDEATGWGLEVSELKKQLEDAKSKGITVRSLVVINPGNPTGQVLAEENQRA 245

Query: 1040 IVKFCKAEGLVLLADEVYQENVYVPEKKFHSFKKVARSMGYGEKDIPLVSFQSVSKGYHG 861
            IV+FCK E LVLLADEVYQENVYV +K FHSFKKVARSMGYGEKDI LVSFQSVSKGYHG
Sbjct: 246  IVEFCKKENLVLLADEVYQENVYVEDKSFHSFKKVARSMGYGEKDISLVSFQSVSKGYHG 305

Query: 860  ECGKRGGYMEVTGFSPEIREQIYKVASVNLCSNISGQILASLVMCPPKVGDESYESYTAE 681
            ECGKRGGYMEVTGFSPE+REQIYKVASVNLCSNISGQILASLVM PPKVGDES+ESY+AE
Sbjct: 306  ECGKRGGYMEVTGFSPEVREQIYKVASVNLCSNISGQILASLVMNPPKVGDESFESYSAE 365

Query: 680  KDGILSSLARRAKTLEDAFNGLEGITCNKAEGAMYLFPRLHLPQNAIKAAETAKTAPDAF 501
            K+GILSSLARRAK LEDAFN LEG+TCNKAEGAMYLFPR+HL Q AIKAAETAKTAPDAF
Sbjct: 366  KNGILSSLARRAKALEDAFNNLEGVTCNKAEGAMYLFPRIHLSQKAIKAAETAKTAPDAF 425

Query: 500  YARRLLNATGIVVVPGSGFRQVPGTWHIRCTILPPEDKIPAIVGRLTEFHKSFMDEFRD 324
            YA+RLL ATGIVVVPGSGF QVPGTWHIRCTILP E+KIPAI+ RLT+FH++FM EFRD
Sbjct: 426  YAKRLLEATGIVVVPGSGFGQVPGTWHIRCTILPQEEKIPAIITRLTDFHQAFMAEFRD 484


>gb|AAR05449.1| alanine aminotransferase [Capsicum annuum]
          Length = 481

 Score =  849 bits (2193), Expect = 0.0
 Identities = 415/477 (87%), Positives = 447/477 (93%)
 Frame = -1

Query: 1760 SITLDSINPKVLECEYAVRGEIVILAQKLQQELLDNPGSHPFDEILYCNIGNPQSLNQQP 1581
            SIT+D+INPKVL+CEYAVRGEIV +AQKLQQ+L DNPGSHPFDEILYCNIGNPQSL QQP
Sbjct: 3    SITIDTINPKVLKCEYAVRGEIVTIAQKLQQDLKDNPGSHPFDEILYCNIGNPQSLAQQP 62

Query: 1580 ITFFREVLALCDHPAILDKSETQGLFSADAIERAWQILDQIPGRATGAYSHSQGIKGLRD 1401
            ITFFREVLALCDHP+ILDKSETQGLFSADAIERA+QILDQIPGRATGAYSHSQGIKGLRD
Sbjct: 63   ITFFREVLALCDHPSILDKSETQGLFSADAIERAFQILDQIPGRATGAYSHSQGIKGLRD 122

Query: 1400 TIAAGIEARDGFPADSNDLFLTDGASPAVHMMMQLLISSKKDGILIPIPQYPLYSASIAL 1221
            TIA+GIEARDGFPAD NDLFLTDGASPAVHMMMQLLI S+ DGIL PIPQYPLYSASIAL
Sbjct: 123  TIASGIEARDGFPADPNDLFLTDGASPAVHMMMQLLIRSQNDGILCPIPQYPLYSASIAL 182

Query: 1220 HGGTLVPYYLDEATGWGLEISELKKQLEDAKSNGITVKALVVINPGNPTGQVLAEENQRD 1041
            HGGTLVPYYLDE TGWGLEISEL+ QL  AKSNGI V+ALVVINPGNPTGQVL E NQR+
Sbjct: 183  HGGTLVPYYLDEQTGWGLEISELEHQLNTAKSNGIDVRALVVINPGNPTGQVLGEANQRE 242

Query: 1040 IVKFCKAEGLVLLADEVYQENVYVPEKKFHSFKKVARSMGYGEKDIPLVSFQSVSKGYHG 861
            IV+FCK EGLVLLADEVYQENVYVP+KKFHSFKK+ RSMGYGEKDI LVSFQSVSKG++G
Sbjct: 243  IVEFCKKEGLVLLADEVYQENVYVPDKKFHSFKKITRSMGYGEKDISLVSFQSVSKGFYG 302

Query: 860  ECGKRGGYMEVTGFSPEIREQIYKVASVNLCSNISGQILASLVMCPPKVGDESYESYTAE 681
            ECGKRGGYME+TGFSPE+REQIYK+ASVNLCSNISGQILASLVM PPKVGDESYES++AE
Sbjct: 303  ECGKRGGYMEITGFSPEVREQIYKLASVNLCSNISGQILASLVMSPPKVGDESYESFSAE 362

Query: 680  KDGILSSLARRAKTLEDAFNGLEGITCNKAEGAMYLFPRLHLPQNAIKAAETAKTAPDAF 501
            K+ +LSSLARRA+ L+DA N LEG+TCN+AEGAMYLFPR++LP  AIKAAE AKTAPDAF
Sbjct: 363  KEAVLSSLARRAQALQDALNSLEGVTCNRAEGAMYLFPRINLPDKAIKAAEVAKTAPDAF 422

Query: 500  YARRLLNATGIVVVPGSGFRQVPGTWHIRCTILPPEDKIPAIVGRLTEFHKSFMDEF 330
            YA+ LLNATGIVVVPGSGFRQVPGTWH RCTILP E+KIPAIV RLTEFHK FMDEF
Sbjct: 423  YAKLLLNATGIVVVPGSGFRQVPGTWHFRCTILPQEEKIPAIVSRLTEFHKKFMDEF 479