BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002443
         (1292 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFW20019.1| antifreeze protein [Daucus carota]                     452   e-125
gb|AAC62932.1| antifreeze protein [Daucus carota] gi|4455920|emb...   451   e-124
gb|AAV66074.1| antifreeze protein [Daucus carota]                     447   e-123
emb|CAA88846.1| polygalacturonase inhibitor [Actinidia deliciosa]     425   e-116
gb|ABU82741.1| polygalacturonase-inhibiting protein [Vitis thunb...   418   e-114

>gb|AFW20019.1| antifreeze protein [Daucus carota]
          Length = 332

 Score =  452 bits (1163), Expect = e-125
 Identities = 220/313 (70%), Positives = 252/313 (80%)
 Frame = +3

Query: 132  LLNPSLSELCNKNDKKVLLQIKKALKNPYLLASWVPTEDCCEWYLVKCDEKTHRINSLIV 311
            L N S S+ CN NDK+ LLQIK ALKNP +  SWV  +DCC W LV+CDE ++RI SLI+
Sbjct: 20   LPNLSASQRCNNNDKQALLQIKTALKNPTITDSWVSDDDCCGWDLVECDETSNRIISLII 79

Query: 312  SDDDKLTGQIPPQVADLPYLETLWFRKLPNLVGPIPSSISKLTNLKSVRLSWTNLSGPVP 491
             DD+ LTGQIPPQV DLPYL+ LWFRKLPNL G IP  IS L +LKS+RLS T+LSGPVP
Sbjct: 80   QDDEALTGQIPPQVGDLPYLQALWFRKLPNLFGKIPEEISALKDLKSLRLSSTSLSGPVP 139

Query: 492  AFFTKLTNLTYLDLSFNKLSGSIPPQLSQLANLEALHLDRNQLTGNIPDTFGSFPGSPDL 671
             FF +LT LT LDLSFNKL G IPPQLS L NL+ALHL++NQLTG IPD FG+F GSPD+
Sbjct: 140  LFFPQLTKLTCLDLSFNKLLGVIPPQLSTLPNLKALHLEQNQLTGEIPDIFGNFAGSPDI 199

Query: 672  YLSHNQLTGSVPKTFARSNPYRIDLSRNKLEGDISFMFTKNKRLETADFSRNMFKFDFSK 851
            YLSHNQLTG VPKTFA S+P R+D S N+LEGDISF+F  NKRLET DFS N+  F+FS+
Sbjct: 200  YLSHNQLTGFVPKTFASSDPIRLDFSGNRLEGDISFLFGPNKRLETLDFSGNVLSFNFSR 259

Query: 852  VQQFPPKLIYLDLNHNQITGSLPSGLSKVELQTFNVSYNRLCGKIPTGGNLDTFDNTAYF 1031
            VQ FPP L YLDLNHNQI+GSL S L+K++LQTFNVS N LCGKIPTGGNL  FD TAY 
Sbjct: 260  VQGFPPSLTYLDLNHNQISGSLSSELAKLDLQTFNVSDNNLCGKIPTGGNLQRFDRTAYL 319

Query: 1032 HNRCLCGSPLPKC 1070
            HN CLCG+PLP+C
Sbjct: 320  HNSCLCGAPLPEC 332


>gb|AAC62932.1| antifreeze protein [Daucus carota] gi|4455920|emb|CAB37347.1|
            antifreeze polypeptide [Daucus carota]
          Length = 332

 Score =  451 bits (1159), Expect = e-124
 Identities = 218/313 (69%), Positives = 252/313 (80%)
 Frame = +3

Query: 132  LLNPSLSELCNKNDKKVLLQIKKALKNPYLLASWVPTEDCCEWYLVKCDEKTHRINSLIV 311
            L N S S+ CN NDK+ LLQIK ALKNP +  SWV  +DCC W LV+CDE ++RI SLI+
Sbjct: 20   LPNLSASQRCNNNDKQALLQIKTALKNPTITDSWVSDDDCCGWDLVECDETSNRIISLII 79

Query: 312  SDDDKLTGQIPPQVADLPYLETLWFRKLPNLVGPIPSSISKLTNLKSVRLSWTNLSGPVP 491
             DD+ LTGQIPPQV DLPYL+ LWFRKLPNL G IP  IS L +LKS+RLS T+LSGPVP
Sbjct: 80   QDDEALTGQIPPQVGDLPYLQALWFRKLPNLFGKIPEEISALKDLKSLRLSSTSLSGPVP 139

Query: 492  AFFTKLTNLTYLDLSFNKLSGSIPPQLSQLANLEALHLDRNQLTGNIPDTFGSFPGSPDL 671
             FF +LT LT LDLSFNKL G IPPQLS L NL+ALHL+RN+LTG IPD FG+F GSPD+
Sbjct: 140  LFFPQLTKLTCLDLSFNKLLGVIPPQLSTLPNLKALHLERNELTGEIPDIFGNFAGSPDI 199

Query: 672  YLSHNQLTGSVPKTFARSNPYRIDLSRNKLEGDISFMFTKNKRLETADFSRNMFKFDFSK 851
            YLSHNQLTG VPKTFAR++P R+D S N+LEGDISF+F   KRLE  DFS N+  F+FS+
Sbjct: 200  YLSHNQLTGFVPKTFARADPIRLDFSGNRLEGDISFLFGPKKRLEMLDFSGNVLSFNFSR 259

Query: 852  VQQFPPKLIYLDLNHNQITGSLPSGLSKVELQTFNVSYNRLCGKIPTGGNLDTFDNTAYF 1031
            VQ+FPP L YLDLNHNQI+GSL S L+K++LQTFNVS N LCGKIPTGGNL  FD TAY 
Sbjct: 260  VQEFPPSLTYLDLNHNQISGSLSSELAKLDLQTFNVSDNNLCGKIPTGGNLQRFDRTAYL 319

Query: 1032 HNRCLCGSPLPKC 1070
            HN CLCG+PLP+C
Sbjct: 320  HNSCLCGAPLPEC 332


>gb|AAV66074.1| antifreeze protein [Daucus carota]
          Length = 332

 Score =  447 bits (1150), Expect = e-123
 Identities = 217/313 (69%), Positives = 251/313 (80%)
 Frame = +3

Query: 132  LLNPSLSELCNKNDKKVLLQIKKALKNPYLLASWVPTEDCCEWYLVKCDEKTHRINSLIV 311
            L N S S+ CN NDK+ LLQIK ALKNP +  SWV  +DCC W LV+CDE ++RI SLI+
Sbjct: 20   LPNLSASQRCNNNDKQALLQIKTALKNPTITDSWVSDDDCCGWDLVECDETSNRIISLII 79

Query: 312  SDDDKLTGQIPPQVADLPYLETLWFRKLPNLVGPIPSSISKLTNLKSVRLSWTNLSGPVP 491
             DD+ LTGQIPPQV DLPYL+ LWFRKLPNL G IP  IS L +LKS+RLS T+LSGPVP
Sbjct: 80   QDDEALTGQIPPQVGDLPYLQALWFRKLPNLFGKIPEEISALKDLKSLRLSSTSLSGPVP 139

Query: 492  AFFTKLTNLTYLDLSFNKLSGSIPPQLSQLANLEALHLDRNQLTGNIPDTFGSFPGSPDL 671
             FF +LT LT LDLSFNKL G IPPQLS LANL+ALHL+RN+LTG IPD FG+F GSPD+
Sbjct: 140  LFFPQLTKLTCLDLSFNKLLGVIPPQLSTLANLKALHLERNELTGEIPDIFGNFAGSPDI 199

Query: 672  YLSHNQLTGSVPKTFARSNPYRIDLSRNKLEGDISFMFTKNKRLETADFSRNMFKFDFSK 851
            YLSHNQLTG VPKTF R++P R+D S N+LEGDISF+F   KRLET DFS N   F+FS+
Sbjct: 200  YLSHNQLTGFVPKTFGRADPIRLDFSGNRLEGDISFLFGPKKRLETLDFSGNELSFNFSR 259

Query: 852  VQQFPPKLIYLDLNHNQITGSLPSGLSKVELQTFNVSYNRLCGKIPTGGNLDTFDNTAYF 1031
            VQ+FPP L YLDLNHNQI+GSL S L+K++LQTFNVS N LCGKIPTGGNL  FD TAY 
Sbjct: 260  VQEFPPSLTYLDLNHNQISGSLSSELAKLDLQTFNVSDNNLCGKIPTGGNLQRFDRTAYL 319

Query: 1032 HNRCLCGSPLPKC 1070
             + CLCG+PLP+C
Sbjct: 320  RDSCLCGAPLPEC 332


>emb|CAA88846.1| polygalacturonase inhibitor [Actinidia deliciosa]
          Length = 327

 Score =  425 bits (1093), Expect = e-116
 Identities = 212/314 (67%), Positives = 248/314 (78%), Gaps = 1/314 (0%)
 Frame = +3

Query: 132  LLNPSLSELCNKNDKKVLLQIKKALKNPYLLASWVPTEDCCEWYLVKCDEKTHRINSLIV 311
            LL+PSLS+ CN NDKKVLL+IK+AL NPYLLASW P  DCC+WY V CD  T+RI +L +
Sbjct: 15   LLSPSLSDRCNPNDKKVLLRIKQALNNPYLLASWNPDNDCCDWYNVDCDLTTNRIIALTI 74

Query: 312  SDDDKLTGQIPPQVADLPYLETLWFRKLPNLVGPIPSSISKLTNLKSVRLSWTNLSGPVP 491
               + ++GQIP  V DLPYL+TL FRKL NL G IPS+ISKL+NLK VRLSWTNLSGPVP
Sbjct: 75   FSGN-ISGQIPAAVGDLPYLQTLIFRKLSNLTGQIPSAISKLSNLKMVRLSWTNLSGPVP 133

Query: 492  AFFTKLTNLTYLDLSFNKLSGSIPPQLSQLANLEALHLDRNQLTGNIPDTFGSFPGS-PD 668
            +FF++L NLT+LDLSFN L+GSIP  LS+L NL+A+HLDRN+LTG IP++FG F G  PD
Sbjct: 134  SFFSQLKNLTFLDLSFNDLTGSIPSSLSKLTNLDAIHLDRNKLTGPIPNSFGEFTGQVPD 193

Query: 669  LYLSHNQLTGSVPKTFARSNPYRIDLSRNKLEGDISFMFTKNKRLETADFSRNMFKFDFS 848
            LYLSHNQLTGS+PKT    N   ID+SRN L GDISFMF  NK ++  DFSRN F+FD S
Sbjct: 194  LYLSHNQLTGSIPKTLGDLNFTVIDVSRNMLSGDISFMFGSNKTIQIVDFSRNKFQFDLS 253

Query: 849  KVQQFPPKLIYLDLNHNQITGSLPSGLSKVELQTFNVSYNRLCGKIPTGGNLDTFDNTAY 1028
            KV  FP  L  LDLNHN+I GSLP GL+K++LQ  NVSYNRLCG IPTGG L  FD T+Y
Sbjct: 254  KV-VFPQSLTSLDLNHNKIYGSLPVGLTKLDLQYLNVSYNRLCGHIPTGGKLQGFDQTSY 312

Query: 1029 FHNRCLCGSPLPKC 1070
            FHNRCLCG+PLP C
Sbjct: 313  FHNRCLCGAPLPDC 326


>gb|ABU82741.1| polygalacturonase-inhibiting protein [Vitis thunbergii]
          Length = 333

 Score =  418 bits (1075), Expect = e-114
 Identities = 207/311 (66%), Positives = 240/311 (77%), Gaps = 1/311 (0%)
 Frame = +3

Query: 141  PSLSELCNKNDKKVLLQIKKALKNPYLLASWVPTEDCCEWYLVKCDEKTHRINSLIVSDD 320
            PSLSE CN  DKKVLLQIKKAL NPY+LASW P  DCCEWY V+CD  THRINSL +   
Sbjct: 24   PSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCEWYCVECDLTTHRINSLTIFSG 83

Query: 321  DKLTGQIPPQVADLPYLETLWFRKLPNLVGPIPSSISKLTNLKSVRLSWTNLSGPVPAFF 500
             +L+GQIP  V DLP+LETL FRKL NL G IP +I+KL +LK VRLSWTNLSGPVPAFF
Sbjct: 84   -QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFF 142

Query: 501  TKLTNLTYLDLSFNKLSGSIPPQLSQLANLEALHLDRNQLTGNIPDTFGSFPGS-PDLYL 677
            ++L NLTYLDLSFN LSG IP  LS L NL+ALHLDRN LTG IPD+FG F GS P LYL
Sbjct: 143  SELKNLTYLDLSFNNLSGPIPGSLSLLPNLDALHLDRNHLTGPIPDSFGKFAGSTPGLYL 202

Query: 678  SHNQLTGSVPKTFARSNPYRIDLSRNKLEGDISFMFTKNKRLETADFSRNMFKFDFSKVQ 857
            SHNQL+G +P +F   +P  +DLSRNKLEGD+S  F  NK  +T DFSRN+F+FD S+V 
Sbjct: 203  SHNQLSGKIPYSFREFDPIVMDLSRNKLEGDLSIFFNANKSTQTVDFSRNLFQFDLSRV- 261

Query: 858  QFPPKLIYLDLNHNQITGSLPSGLSKVELQTFNVSYNRLCGKIPTGGNLDTFDNTAYFHN 1037
            +FP  L  LDL+HN+I GSLP  ++ ++LQ  NVSYNRLCGKIP GG L +FD  +YFHN
Sbjct: 262  EFPKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHN 321

Query: 1038 RCLCGSPLPKC 1070
            RCLCG+PL  C
Sbjct: 322  RCLCGAPLQSC 332