BLASTX nr result
ID: Bupleurum21_contig00002423
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00002423
(1499 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAD29679.1|AF133209_1 CLC-Nt2 protein [Nicotiana tabacum] 587 e-165
ref|XP_002300101.1| Cl-channel clc-7 [Populus trichocarpa] gi|22... 582 e-163
ref|XP_002273594.2| PREDICTED: chloride channel protein CLC-b [V... 581 e-163
ref|XP_002509531.1| chloride channel clc, putative [Ricinus comm... 580 e-163
gb|ADK66982.1| chloride channel ClC4 [Vitis vinifera] 578 e-162
>gb|AAD29679.1|AF133209_1 CLC-Nt2 protein [Nicotiana tabacum]
Length = 786
Score = 587 bits (1514), Expect = e-165
Identities = 291/380 (76%), Positives = 312/380 (82%)
Frame = +2
Query: 359 WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 538
WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV
Sbjct: 235 WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 294
Query: 539 VVVLRLSMEYCKYGNCGLFGQGGLILFDVSDVSLRYHLIDFIPXXXXXXXXXXLGSLYNH 718
VV+LR +EYCK GNCGLFG+GGLI+FDVS VS+ YH++D IP LGSLYNH
Sbjct: 295 VVILRAFIEYCKSGNCGLFGRGGLIMFDVSGVSVSYHVVDIIPVVVIGIIGGLLGSLYNH 354
Query: 719 LLHKILRLYNIINEXXXXXXXXXXXXXXXXXXACLYGLPFLAGCTPCDPSLQEACPTTGR 898
+LHKILRLYN+INE C+YGLPFLA C PCDPSL +CP TG
Sbjct: 355 VLHKILRLYNLINEKGKLHKVLLALSVSLFTSICMYGLPFLAKCKPCDPSLPGSCPGTGG 414
Query: 899 VGNFRQFNCPSGHYNDLATLLHTTNDDAVRNIFSTNTSTEFKYFSLIIFFVLYCILGLFT 1078
GNF+QFNCP G+YNDLATLL TTNDDAVRNIFS NT EF+ SLI +FVLYCILGL T
Sbjct: 415 TGNFKQFNCPDGYYNDLATLLLTTNDDAVRNIFSINTPGEFQVMSLISYFVLYCILGLIT 474
Query: 1079 FGIAVPSGLFLPIILMGSAYGRMLGIAMGDYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 1258
FGIAVPSGLFLPIILMGSAYGR+L IAMG YTKID GLYAVLGAASLMAGSMRMTVSLCV
Sbjct: 475 FGIAVPSGLFLPIILMGSAYGRLLAIAMGSYTKIDPGLYAVLGAASLMAGSMRMTVSLCV 534
Query: 1259 IFXXXXXXXXXXPITMFVLLIAKTVGDCFNPSIYDIILDLKGLPFLDAHPEPWMRNITVG 1438
IF PITM VLLIAK+VGDCFN SIY+IIL+LKGLPFLDA+PEPWMRNIT G
Sbjct: 535 IFLELTNNLLLLPITMLVLLIAKSVGDCFNLSIYEIILELKGLPFLDANPEPWMRNITAG 594
Query: 1439 ELADVKPPVVTLRGIEKVGR 1498
ELADVKPPVVTL G+EKVGR
Sbjct: 595 ELADVKPPVVTLCGVEKVGR 614
Score = 130 bits (327), Expect = 9e-28
Identities = 62/71 (87%), Positives = 67/71 (94%)
Frame = +1
Query: 1 ANFLLTLAASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTLMVKIVGSIGAVSAG 180
ANF+LTL A++LCVCFAPTAAGPGIPEIKAYLNGVDTPNM+GATTL VKI+GSI AVSA
Sbjct: 142 ANFVLTLIAALLCVCFAPTAAGPGIPEIKAYLNGVDTPNMYGATTLFVKIIGSIAAVSAS 201
Query: 181 LDLGKEGPLVH 213
LDLGKEGPLVH
Sbjct: 202 LDLGKEGPLVH 212
>ref|XP_002300101.1| Cl-channel clc-7 [Populus trichocarpa] gi|222847359|gb|EEE84906.1|
Cl-channel clc-7 [Populus trichocarpa]
Length = 785
Score = 582 bits (1499), Expect = e-163
Identities = 286/380 (75%), Positives = 311/380 (81%)
Frame = +2
Query: 359 WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 538
WLRYFNNDRDRRD+ITCG+SSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV
Sbjct: 234 WLRYFNNDRDRRDIITCGASSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 293
Query: 539 VVVLRLSMEYCKYGNCGLFGQGGLILFDVSDVSLRYHLIDFIPXXXXXXXXXXLGSLYNH 718
VV+LR +E C G CGLFG+GGLI+FDVSDV + YH++D IP LGSLYN+
Sbjct: 294 VVILRTFIEICNSGKCGLFGKGGLIMFDVSDVVVTYHVMDVIPITIIGILGGILGSLYNY 353
Query: 719 LLHKILRLYNIINEXXXXXXXXXXXXXXXXXXACLYGLPFLAGCTPCDPSLQEACPTTGR 898
LLHK+L +YN+IN+ CLYGLPFLA C PCDPS+QE CPT R
Sbjct: 354 LLHKVLVVYNLINQKGRIHKLLLALTVSIFTSVCLYGLPFLAKCQPCDPSVQEICPTNSR 413
Query: 899 VGNFRQFNCPSGHYNDLATLLHTTNDDAVRNIFSTNTSTEFKYFSLIIFFVLYCILGLFT 1078
GNF+QFNCP GHYNDLATLL TTNDDAVRNIFS+N EF+ SL+IFFVLYCILGLFT
Sbjct: 414 SGNFKQFNCPDGHYNDLATLLFTTNDDAVRNIFSSNALKEFQPASLLIFFVLYCILGLFT 473
Query: 1079 FGIAVPSGLFLPIILMGSAYGRMLGIAMGDYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 1258
FGIAVPSGLFLPIILMGSAYGR+LGIAMG YTKIDQGLYAVLGAASLMAGSMRMTVSLCV
Sbjct: 474 FGIAVPSGLFLPIILMGSAYGRLLGIAMGSYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 533
Query: 1259 IFXXXXXXXXXXPITMFVLLIAKTVGDCFNPSIYDIILDLKGLPFLDAHPEPWMRNITVG 1438
IF PITM VLLI+KTVGD FNPSIY+IILDLKGLPFLDA+PEPWMRN+TV
Sbjct: 534 IFLELTNNLLLLPITMIVLLISKTVGDSFNPSIYEIILDLKGLPFLDANPEPWMRNLTVA 593
Query: 1439 ELADVKPPVVTLRGIEKVGR 1498
ELAD KPPVVTL G+EKV R
Sbjct: 594 ELADAKPPVVTLCGVEKVSR 613
Score = 134 bits (337), Expect = 6e-29
Identities = 66/71 (92%), Positives = 67/71 (94%)
Frame = +1
Query: 1 ANFLLTLAASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTLMVKIVGSIGAVSAG 180
AN LLTL ASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFG TTL+VKI GSIGAVSAG
Sbjct: 141 ANLLLTLFASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGVTTLIVKIFGSIGAVSAG 200
Query: 181 LDLGKEGPLVH 213
LDLGKEGPLVH
Sbjct: 201 LDLGKEGPLVH 211
>ref|XP_002273594.2| PREDICTED: chloride channel protein CLC-b [Vitis vinifera]
gi|296082356|emb|CBI21361.3| unnamed protein product
[Vitis vinifera]
Length = 789
Score = 581 bits (1497), Expect = e-163
Identities = 285/380 (75%), Positives = 310/380 (81%)
Frame = +2
Query: 359 WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 538
WLRYFNNDRDRRDLITCG+SSGVCAAFR+PVGGVLF+LEEVATWWRSALLWRTFFSTAVV
Sbjct: 238 WLRYFNNDRDRRDLITCGASSGVCAAFRAPVGGVLFSLEEVATWWRSALLWRTFFSTAVV 297
Query: 539 VVVLRLSMEYCKYGNCGLFGQGGLILFDVSDVSLRYHLIDFIPXXXXXXXXXXLGSLYNH 718
VVLR +EYC G CGLFG+GGLI+FDVSDV++ YH +D P LGSLYNH
Sbjct: 298 AVVLRAFIEYCNSGKCGLFGRGGLIMFDVSDVTVTYHAMDIAPVALIGLIGGVLGSLYNH 357
Query: 719 LLHKILRLYNIINEXXXXXXXXXXXXXXXXXXACLYGLPFLAGCTPCDPSLQEACPTTGR 898
LLHK+LR+YN+IN+ CLY LPFLA C+PCD S+ E CPT GR
Sbjct: 358 LLHKVLRVYNLINQKGKIHKLLLSLSVSLFTSICLYCLPFLATCSPCDSSITETCPTNGR 417
Query: 899 VGNFRQFNCPSGHYNDLATLLHTTNDDAVRNIFSTNTSTEFKYFSLIIFFVLYCILGLFT 1078
GNF+QFNCP G+YNDLA+LL TTNDDAVRNIFSTNT+TEF SL+IFF LY ILGL T
Sbjct: 418 TGNFKQFNCPDGYYNDLASLLFTTNDDAVRNIFSTNTATEFHPLSLLIFFGLYYILGLIT 477
Query: 1079 FGIAVPSGLFLPIILMGSAYGRMLGIAMGDYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 1258
FGIAVPSGLFLPIILMGSAYGR+LGIAMG YTKIDQGLYAVLGAASLMAGSMRMTVSLCV
Sbjct: 478 FGIAVPSGLFLPIILMGSAYGRLLGIAMGSYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 537
Query: 1259 IFXXXXXXXXXXPITMFVLLIAKTVGDCFNPSIYDIILDLKGLPFLDAHPEPWMRNITVG 1438
IF PITM VLLIAK+VGDC NPSIYDIIL LKGLPFLDA+PEPWMRN+TVG
Sbjct: 538 IFLELTNNLLLLPITMIVLLIAKSVGDCINPSIYDIILHLKGLPFLDANPEPWMRNLTVG 597
Query: 1439 ELADVKPPVVTLRGIEKVGR 1498
ELAD KPPVVTLRG+EKV R
Sbjct: 598 ELADAKPPVVTLRGVEKVAR 617
Score = 132 bits (332), Expect = 2e-28
Identities = 64/71 (90%), Positives = 68/71 (95%)
Frame = +1
Query: 1 ANFLLTLAASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTLMVKIVGSIGAVSAG 180
ANF+LTL A+ LCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGA+TL+VKI GSIGAVSAG
Sbjct: 145 ANFVLTLFAAFLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGASTLIVKIFGSIGAVSAG 204
Query: 181 LDLGKEGPLVH 213
LDLGKEGPLVH
Sbjct: 205 LDLGKEGPLVH 215
>ref|XP_002509531.1| chloride channel clc, putative [Ricinus communis]
gi|223549430|gb|EEF50918.1| chloride channel clc,
putative [Ricinus communis]
Length = 787
Score = 580 bits (1496), Expect = e-163
Identities = 282/380 (74%), Positives = 312/380 (82%)
Frame = +2
Query: 359 WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 538
WLRYFNNDRDRRD+ITCGSSSGVCAAFR+PVGGVLFALEEVATWWRSALLWRTFFSTAVV
Sbjct: 236 WLRYFNNDRDRRDIITCGSSSGVCAAFRAPVGGVLFALEEVATWWRSALLWRTFFSTAVV 295
Query: 539 VVVLRLSMEYCKYGNCGLFGQGGLILFDVSDVSLRYHLIDFIPXXXXXXXXXXLGSLYNH 718
VV+LR +E CK G CGLFG+GGLI+FDVSDV++ YH++D +P LGSLYN+
Sbjct: 296 VVILRAFIEICKSGKCGLFGKGGLIMFDVSDVTVSYHVMDILPVIIIGIIGGLLGSLYNY 355
Query: 719 LLHKILRLYNIINEXXXXXXXXXXXXXXXXXXACLYGLPFLAGCTPCDPSLQEACPTTGR 898
LLHK+LRLYN+IN+ CLYGLPFLA C PCDPS+ E CPT R
Sbjct: 356 LLHKVLRLYNLINQKGKMHKLLLSLTVSLFTSVCLYGLPFLAKCQPCDPSVTELCPTNDR 415
Query: 899 VGNFRQFNCPSGHYNDLATLLHTTNDDAVRNIFSTNTSTEFKYFSLIIFFVLYCILGLFT 1078
GNF+QFNCP GHYNDLATLL TTNDDAVRNIFS+NT EF+ +L+IFF LYC+LGLFT
Sbjct: 416 SGNFKQFNCPKGHYNDLATLLLTTNDDAVRNIFSSNTPHEFQPATLLIFFALYCVLGLFT 475
Query: 1079 FGIAVPSGLFLPIILMGSAYGRMLGIAMGDYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 1258
FGIAVPSGLFLPIILMGSAYGR+LG+AMG YT +DQGLYAVLGAASLMAGSMRMTVSLCV
Sbjct: 476 FGIAVPSGLFLPIILMGSAYGRLLGVAMGSYTNLDQGLYAVLGAASLMAGSMRMTVSLCV 535
Query: 1259 IFXXXXXXXXXXPITMFVLLIAKTVGDCFNPSIYDIILDLKGLPFLDAHPEPWMRNITVG 1438
IF PITM VLLIAKTVGD FNPSIY+IIL LKGLPFLDA+PEPWMRN+TVG
Sbjct: 536 IFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYEIILHLKGLPFLDANPEPWMRNLTVG 595
Query: 1439 ELADVKPPVVTLRGIEKVGR 1498
ELAD KPP+VTL G+EKV R
Sbjct: 596 ELADAKPPLVTLCGVEKVSR 615
Score = 124 bits (311), Expect = 6e-26
Identities = 60/70 (85%), Positives = 64/70 (91%)
Frame = +1
Query: 4 NFLLTLAASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTLMVKIVGSIGAVSAGL 183
N +LT AS LCV FAPTAAGPGIPEIKAYLNG+DTPNMFGATTL+VKI GSIGAV+AGL
Sbjct: 144 NLILTAFASALCVWFAPTAAGPGIPEIKAYLNGIDTPNMFGATTLIVKIFGSIGAVAAGL 203
Query: 184 DLGKEGPLVH 213
DLGKEGPLVH
Sbjct: 204 DLGKEGPLVH 213
>gb|ADK66982.1| chloride channel ClC4 [Vitis vinifera]
Length = 789
Score = 578 bits (1489), Expect = e-162
Identities = 284/380 (74%), Positives = 309/380 (81%)
Frame = +2
Query: 359 WLRYFNNDRDRRDLITCGSSSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVV 538
WLRYFNNDRDRRDLITCG+SSGVCAAFR+PVGGVLF+LEEVATWWRSALLWRTFFSTAVV
Sbjct: 238 WLRYFNNDRDRRDLITCGASSGVCAAFRAPVGGVLFSLEEVATWWRSALLWRTFFSTAVV 297
Query: 539 VVVLRLSMEYCKYGNCGLFGQGGLILFDVSDVSLRYHLIDFIPXXXXXXXXXXLGSLYNH 718
VVLR +EYC G CGLFG+GGLI+FDVSDV++ YH +D P LGSLYNH
Sbjct: 298 AVVLRAFIEYCNSGKCGLFGRGGLIMFDVSDVTVTYHAMDIAPVALIGLIGGVLGSLYNH 357
Query: 719 LLHKILRLYNIINEXXXXXXXXXXXXXXXXXXACLYGLPFLAGCTPCDPSLQEACPTTGR 898
LLHK+LR+YN+IN+ CLY LPFLA C+PCD S+ E CPT GR
Sbjct: 358 LLHKVLRVYNLINQKGKIHKLLLSLSVSLFTSICLYCLPFLATCSPCDSSITETCPTNGR 417
Query: 899 VGNFRQFNCPSGHYNDLATLLHTTNDDAVRNIFSTNTSTEFKYFSLIIFFVLYCILGLFT 1078
GNF+QFNCP G+YNDLA+LL TTNDDAVRNIFSTNT+TEF SL+IFF LY ILGL T
Sbjct: 418 TGNFKQFNCPDGYYNDLASLLFTTNDDAVRNIFSTNTATEFHPLSLLIFFGLYYILGLIT 477
Query: 1079 FGIAVPSGLFLPIILMGSAYGRMLGIAMGDYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 1258
FGIAVPSGLFLPIILMGSAYGR+LGIAMG YTKIDQGLYAVLGAASLMAGSMRMTVSLCV
Sbjct: 478 FGIAVPSGLFLPIILMGSAYGRLLGIAMGSYTKIDQGLYAVLGAASLMAGSMRMTVSLCV 537
Query: 1259 IFXXXXXXXXXXPITMFVLLIAKTVGDCFNPSIYDIILDLKGLPFLDAHPEPWMRNITVG 1438
IF PITM VLLIAK+VGDC NPSIYDIIL LKGLPFLDA+PEPWMRN+TVG
Sbjct: 538 IFLELTNNLLLLPITMIVLLIAKSVGDCINPSIYDIILHLKGLPFLDANPEPWMRNLTVG 597
Query: 1439 ELADVKPPVVTLRGIEKVGR 1498
ELAD KP VVTLRG+EKV R
Sbjct: 598 ELADAKPQVVTLRGVEKVAR 617
Score = 132 bits (332), Expect = 2e-28
Identities = 64/71 (90%), Positives = 68/71 (95%)
Frame = +1
Query: 1 ANFLLTLAASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTLMVKIVGSIGAVSAG 180
ANF+LTL A+ LCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGA+TL+VKI GSIGAVSAG
Sbjct: 145 ANFVLTLFAAFLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGASTLIVKIFGSIGAVSAG 204
Query: 181 LDLGKEGPLVH 213
LDLGKEGPLVH
Sbjct: 205 LDLGKEGPLVH 215