BLASTX nr result
ID: Bupleurum21_contig00002346
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00002346
(1548 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285825.1| PREDICTED: eukaryotic translation initiation... 479 e-132
ref|XP_004169059.1| PREDICTED: LOW QUALITY PROTEIN: eukaryotic t... 459 e-127
ref|XP_004150695.1| PREDICTED: eukaryotic translation initiation... 459 e-127
ref|XP_002331282.1| predicted protein [Populus trichocarpa] gi|2... 445 e-122
ref|XP_003552707.1| PREDICTED: eukaryotic translation initiation... 427 e-117
>ref|XP_002285825.1| PREDICTED: eukaryotic translation initiation factor 5-like [Vitis
vinifera]
Length = 450
Score = 479 bits (1232), Expect = e-132
Identities = 261/447 (58%), Positives = 293/447 (65%), Gaps = 6/447 (1%)
Frame = -1
Query: 1323 MALQNIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPAAYTTKYFG 1144
MALQNIGAGN+DDAFYRYKMP+MITKIEGRGNGIKTN+VNMVDIAKAL RPA+YTTKYFG
Sbjct: 1 MALQNIGAGNRDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60
Query: 1143 CELGAQSKFDEKSATSHVNGSHDTGKLAALLENFIKKYVQCYGCGNPETEIIITKTQMIT 964
CELGAQSKFDE++ TSHVNG+HDT KLA LLENFIKKYVQCYGCGNPETEI+ITKTQMIT
Sbjct: 61 CELGAQSKFDERTGTSHVNGAHDTPKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMIT 120
Query: 963 LKCAACGHLSDVDMRDKLTTFIIKNPP-XXXXXXXXXXXXXXXXXXXXAGEAVD-----X 802
LKCAACG +SDVDMRDKLTTFI+KNPP GEA D
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKTSKDKKAMRRAEKERLKEGEAADEEQKKL 180
Query: 801 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSDEDNSPAGSQVDEMEAAAGNDDDNDVQWQ 622
SDED SP GSQ DE E ++ D+DVQW+
Sbjct: 181 KKEAVKKKGSSVSSKDVTSKVASTKKTTSVSDEDRSPTGSQADENETEPADESDDDVQWK 240
Query: 621 TDTSAEAARQRIQEQLSAVTAGMVMLDTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 442
TD S EAA+QRI+EQLSAVTAGMVML
Sbjct: 241 TDPSVEAAQQRIKEQLSAVTAGMVMLS---------------------------MDEEKA 273
Query: 441 XXXXXXXAREEKPEFNGQTNGAADSSTPHEKLVLEVKNYLKKGSPASKFKPFLGTLSGTS 262
EEK E G NG S E+LV E+K YLKKG A K K FLG+LSGTS
Sbjct: 274 KSTKKSPKHEEKSELKGAQNG-TKSGNNQERLVDEIKEYLKKGPSAVKLKNFLGSLSGTS 332
Query: 261 QQVFDALFEAMFDGAGKGFSSEVTKKKNYLAAATQDEGSQLLLLNALETFCGNASSEAVK 82
Q++ DALFEA+F G GKG + EV KKK YLAA TQ++ SQ +LL A+E+FCG ++ EAVK
Sbjct: 333 QEIIDALFEALFMGIGKGLAKEVAKKKTYLAAVTQEDRSQSILLRAIESFCGKSNPEAVK 392
Query: 81 EVAFALKALYDNDVLEEEFIVEWYKNG 1
EV LKALYDND+LEEEFI+EWYK G
Sbjct: 393 EVGLVLKALYDNDLLEEEFILEWYKQG 419
>ref|XP_004169059.1| PREDICTED: LOW QUALITY PROTEIN: eukaryotic translation initiation
factor 5-like [Cucumis sativus]
Length = 444
Score = 459 bits (1182), Expect = e-127
Identities = 250/444 (56%), Positives = 292/444 (65%), Gaps = 3/444 (0%)
Frame = -1
Query: 1323 MALQNIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPAAYTTKYFG 1144
MALQNIGA N+DDAFYRYKMP+M+TKIEGRGNGIKTN+VNMV+IAKALGRPA+YTTKYFG
Sbjct: 1 MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
Query: 1143 CELGAQSKFDEKSATSHVNGSHDTGKLAALLENFIKKYVQCYGCGNPETEIIITKTQMIT 964
CELGAQSKFDEK+ TS VNG+H+T KLA LLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61 CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
Query: 963 LKCAACGHLSDVDMRDKLTTFIIKNPP-XXXXXXXXXXXXXXXXXXXXAGEAVD--XXXX 793
+KCAACG +SDVDMRDKLTTFI+KNPP GEA D
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKX 180
Query: 792 XXXXXXXXXXXXXXXXXXXXXXXXXXXSDEDNSPAGSQVDEMEAAAGNDDDNDVQWQTDT 613
SDED+SP S D+ + AA ++DD+DVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
Query: 612 SAEAARQRIQEQLSAVTAGMVMLDTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 433
S EAA+QRIQEQLSAVTA MVML T
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLST-----------------------------TEENKS 271
Query: 432 XXXXAREEKPEFNGQTNGAADSSTPHEKLVLEVKNYLKKGSPASKFKPFLGTLSGTSQQV 253
+ EK NG NG S+ H L+ E+K+YLKKG+ + K FL +L+GT Q++
Sbjct: 272 SKKSSEHEKSAANGNKNGNGVST--HGALIQEIKDYLKKGASTTDLKSFLESLAGTRQEI 329
Query: 252 FDALFEAMFDGAGKGFSSEVTKKKNYLAAATQDEGSQLLLLNALETFCGNASSEAVKEVA 73
+AL EA+FDG GKGFS EV KKK +LA EGSQ++LL A+E+FC N S EA KEVA
Sbjct: 330 VNALVEALFDGVGKGFSKEVVKKKKFLAVVAAVEGSQIILLRAIESFCLNTSPEAGKEVA 389
Query: 72 FALKALYDNDVLEEEFIVEWYKNG 1
LK+LYD DV+EEEF++EWY+ G
Sbjct: 390 LVLKSLYDGDVIEEEFVLEWYQQG 413
>ref|XP_004150695.1| PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis
sativus]
Length = 444
Score = 459 bits (1182), Expect = e-127
Identities = 249/444 (56%), Positives = 291/444 (65%), Gaps = 3/444 (0%)
Frame = -1
Query: 1323 MALQNIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPAAYTTKYFG 1144
MALQNIGA N+DDAFYRYKMP+M+TKIEGRGNGIKTN+VNMV+IAKALGRPA+YTTKYFG
Sbjct: 1 MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
Query: 1143 CELGAQSKFDEKSATSHVNGSHDTGKLAALLENFIKKYVQCYGCGNPETEIIITKTQMIT 964
CELGAQSKFDEK+ TS VNG+H+T KLA LLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61 CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
Query: 963 LKCAACGHLSDVDMRDKLTTFIIKNPP-XXXXXXXXXXXXXXXXXXXXAGEAVD--XXXX 793
+KCAACG +SDVDMRDKLTTFI+KNPP GEA D
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
Query: 792 XXXXXXXXXXXXXXXXXXXXXXXXXXXSDEDNSPAGSQVDEMEAAAGNDDDNDVQWQTDT 613
SDED+SP S D+ + AA ++DD+DVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
Query: 612 SAEAARQRIQEQLSAVTAGMVMLDTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 433
S EAA+QRIQEQLSAVTA MVML T
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLST-----------------------------TEENKS 271
Query: 432 XXXXAREEKPEFNGQTNGAADSSTPHEKLVLEVKNYLKKGSPASKFKPFLGTLSGTSQQV 253
+ EK NG NG S+ H L+ E+K+YLKKG+ + K FL +L+GT Q++
Sbjct: 272 SKKSSEHEKSAANGNKNGNGVST--HGALIQEIKDYLKKGASTTDLKSFLESLAGTRQEI 329
Query: 252 FDALFEAMFDGAGKGFSSEVTKKKNYLAAATQDEGSQLLLLNALETFCGNASSEAVKEVA 73
+AL EA+FDG GKGFS EV KKK +LA EGSQ++LL A+E+FC N S EA KEVA
Sbjct: 330 VNALVEALFDGVGKGFSKEVVKKKKFLAVVAAVEGSQIILLRAIESFCLNTSPEAGKEVA 389
Query: 72 FALKALYDNDVLEEEFIVEWYKNG 1
LK+LYD DV+EEEF++EWY+ G
Sbjct: 390 LVLKSLYDGDVIEEEFVLEWYQQG 413
>ref|XP_002331282.1| predicted protein [Populus trichocarpa] gi|222873707|gb|EEF10838.1|
predicted protein [Populus trichocarpa]
Length = 444
Score = 445 bits (1145), Expect = e-122
Identities = 253/445 (56%), Positives = 285/445 (64%), Gaps = 4/445 (0%)
Frame = -1
Query: 1323 MALQNIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPAAYTTKYFG 1144
MALQNIGA N DDAFYRYKMP+MITKIEGRGNGIKTNIVNMVDIAKAL RPA+YTTKYFG
Sbjct: 1 MALQNIGAANSDDAFYRYKMPKMITKIEGRGNGIKTNIVNMVDIAKALARPASYTTKYFG 60
Query: 1143 CELGAQSKFDEKSATSHVNGSHDTGKLAALLENFIKKYVQCYGCGNPETEIIITKTQMIT 964
CELGAQSKFDEK+ TSHVNGSHDT KLA LLENFIKKYVQCYGCGNPETE+IITK+QMI
Sbjct: 61 CELGAQSKFDEKTGTSHVNGSHDTAKLAGLLENFIKKYVQCYGCGNPETEVIITKSQMIQ 120
Query: 963 LKCAACGHLSDVDMRDKLTTFIIKNPP-XXXXXXXXXXXXXXXXXXXXAGEAVD--XXXX 793
LKCAACG +SDVDMRDKLTTFI+KNPP GEA D
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPETKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180
Query: 792 XXXXXXXXXXXXXXXXXXXXXXXXXXXSDEDNSPAGSQVDEMEAAAGNDDDNDVQWQTDT 613
+E SP SQVDE E A DD++DVQWQTDT
Sbjct: 181 KNGKKKGSSTKDGHAKASSIKKKANSSDEERGSPTHSQVDEKEEA---DDEDDVQWQTDT 237
Query: 612 SAEAARQRIQEQLSAVTAGMVMLDTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 433
S EAARQRIQEQLSA TA MVML
Sbjct: 238 SLEAARQRIQEQLSAATADMVML-----------------------AADETEKNSKTASK 274
Query: 432 XXXXAREEKPEFNGQTNGAADSSTPHEKLVLEVKNYLKKGSPASKFKPFLGTLSGTSQQV 253
REE+P+ +S+ +E LV EVK LKKG ASK + LG+L G++Q+
Sbjct: 275 ENGSPREEEPK------AGCGNSSAYETLVEEVKANLKKGVSASKIQSVLGSLDGSAQEK 328
Query: 252 FDALFEAMFDGAGKGFSSEVTKKKNYLAAA-TQDEGSQLLLLNALETFCGNASSEAVKEV 76
+ LFEA+F+G KGF+ EV KKK YLAAA QDEGSQLLLL A+ FCG +SS A+KEV
Sbjct: 329 MNGLFEALFEGVEKGFAKEVGKKKKYLAAAVAQDEGSQLLLLRAIGAFCGKSSSSALKEV 388
Query: 75 AFALKALYDNDVLEEEFIVEWYKNG 1
A +K LYD DVLEEE++V+WY+ G
Sbjct: 389 ALVMKDLYDADVLEEEYMVQWYQEG 413
>ref|XP_003552707.1| PREDICTED: eukaryotic translation initiation factor 5-like [Glycine
max]
Length = 439
Score = 427 bits (1099), Expect = e-117
Identities = 247/446 (55%), Positives = 277/446 (62%), Gaps = 5/446 (1%)
Frame = -1
Query: 1323 MALQNIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPAAYTTKYFG 1144
MALQNIGA N DDAFYRYKMPRMITKIEGRGNGIKTN+VNMVDIAKAL RPA+YTTKYFG
Sbjct: 1 MALQNIGAANSDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60
Query: 1143 CELGAQSKFDEKSATSHVNGSHDTGKLAALLENFIKKYVQCYGCGNPETEIIITKTQMIT 964
CELGAQSKFDEK+ TSHVNG+HDT KLA LLENFIKKYVQCYGCGNPETEIIITK QMI
Sbjct: 61 CELGAQSKFDEKTGTSHVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKNQMIQ 120
Query: 963 LKCAACGHLSDVDMRDKLTTFIIKNPP-XXXXXXXXXXXXXXXXXXXXAGEAVD--XXXX 793
LKCAACG +SDVDMRDKLTTFIIKNPP GE D
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIIKNPPEVKKGSKDKKAMRRAEKERLKEGELADEEQKKV 180
Query: 792 XXXXXXXXXXXXXXXXXXXXXXXXXXXSDED-NSPAGSQVDEMEAAAGNDDDNDVQWQTD 616
SDED SP SQ+DE E A DDD+DVQWQTD
Sbjct: 181 KKEVKKKGSSSSKDGTKSTSSKKKASGSDEDRTSPTHSQIDEKEEALDEDDDDDVQWQTD 240
Query: 615 TSAEAARQRIQEQLSAVTAGMVMLDTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 436
TS +AARQRIQEQLSAVTA MVML T
Sbjct: 241 TSLDAARQRIQEQLSAVTADMVMLSTDEP------------------------------- 269
Query: 435 XXXXXAREEKPEFNGQTNGAADSSTPH-EKLVLEVKNYLKKGSPASKFKPFLGTLSGTSQ 259
E+K E + +G+ + ++ H LV EVK LKKG A++ L +Q
Sbjct: 270 -------EKKKEASNGNDGSQNGNSMHCRTLVGEVKANLKKGVGANELLSQLAAHPVPAQ 322
Query: 258 QVFDALFEAMFDGAGKGFSSEVTKKKNYLAAATQDEGSQLLLLNALETFCGNASSEAVKE 79
+ AL EA+F+G KGF+ E KKKNY + A DEGSQ LLL+A+E F ++S A+KE
Sbjct: 323 EKMSALVEALFEGTEKGFAKEADKKKNYFSVAVADEGSQPLLLHAIEEFSCKSTSNALKE 382
Query: 78 VAFALKALYDNDVLEEEFIVEWYKNG 1
VA LKALYD DVLEEE IV+WY+ G
Sbjct: 383 VALVLKALYDADVLEEEHIVQWYQKG 408