BLASTX nr result
ID: Bupleurum21_contig00002252
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00002252
(1671 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002523869.1| Aspartic proteinase nepenthesin-2 precursor,... 531 e-148
ref|XP_002272243.1| PREDICTED: aspartic proteinase nepenthesin-2... 517 e-144
ref|XP_002309394.1| predicted protein [Populus trichocarpa] gi|2... 493 e-137
ref|XP_002312826.1| predicted protein [Populus trichocarpa] gi|2... 488 e-135
ref|XP_003520712.1| PREDICTED: aspartic proteinase nepenthesin-2... 487 e-135
>ref|XP_002523869.1| Aspartic proteinase nepenthesin-2 precursor, putative [Ricinus
communis] gi|223536957|gb|EEF38595.1| Aspartic proteinase
nepenthesin-2 precursor, putative [Ricinus communis]
Length = 447
Score = 531 bits (1367), Expect = e-148
Identities = 255/436 (58%), Positives = 332/436 (76%), Gaps = 2/436 (0%)
Frame = +3
Query: 165 ITLPLKLINTNLT-SQSQYQKLYYLASLSQARAHHLKTPKPQQQFSNTQLFPRSYGGYSI 341
I++PL TN SQ QKL YL S S ARAHHLK P+ T +F SYGGYSI
Sbjct: 26 ISIPLSHSYTNQNPSQDHLQKLNYLVSTSLARAHHLKNPQ------TTPVFSHSYGGYSI 79
Query: 342 SLNIGTPPQTIPFVMDTGSDFVWFPCTRKYICRNCTFPATQPPPVFIPKQSSTSKVLGCL 521
SL+ GTPPQT+ FVMDTGS FVWFPCT +Y+C NC+F + P F+PK SS+SK++GC
Sbjct: 80 SLSFGTPPQTLSFVMDTGSSFVWFPCTLRYLCNNCSFTSRISP--FLPKHSSSSKIIGCK 137
Query: 522 NKKCGWVHRDQDVRTRCSECVNNIKNCSQICPPYIIMYGSGSTGGVSIVDSLNLPGKNVP 701
N KC W+H+ RC++C NN +NCSQICPPY+I+YGSG+TGGV++ ++L+L G VP
Sbjct: 138 NPKCSWIHQTD---LRCTDCDNNSRNCSQICPPYLILYGSGTTGGVALSETLHLHGLIVP 194
Query: 702 DFLVGCSVFSSRQPAGIAGFGRGPSSLPNQLGLKKFSYCLLSHKFDDSPESSSLVLYSGK 881
+FLVGCSVFSSRQPAGIAGFGRGPSSLP+QLGL KFSYCLLSHKFDD+ ESSSLVL S
Sbjct: 195 NFLVGCSVFSSRQPAGIAGFGRGPSSLPSQLGLTKFSYCLLSHKFDDTQESSSLVLDSQS 254
Query: 882 DSDQKTSKLSYTPILKFPEIQDKQAYTTFSGYYYLGLRKILVGGKKIKIPFKYLTPGPTG 1061
DSD+KT+ L YTP++K P++QDK A FS YYY+ LR+I +GG+ +KIP+KYL+P G
Sbjct: 255 DSDKKTAALMYTPLVKNPKVQDKPA---FSVYYYVSLRRISIGGRSVKIPYKYLSPDKDG 311
Query: 1062 DGGTIVDSGSTFTFLTKNVHELVVSALVEQVKDYKRAKQVESITGLSPCFDISGHRSVNF 1241
+GGTI+DSG+TFT+++ E++ + + QVK+Y+RA VE+++GL PCF++SG + +
Sbjct: 312 NGGTIIDSGTTFTYMSTEAFEILSNEFISQVKNYERALMVEALSGLKPCFNVSGAKELEL 371
Query: 1242 PEMKFHFKGGAEMVLPLVNYFSFVSKELEVVCLTMVTD-AGNSGGPAIILGNFQLQNFYT 1418
P+++ HFKGGA++ LPL NYF+F+ EV C T+VTD A + GP +ILGNFQ+QNFY
Sbjct: 372 PQLRLHFKGGADVELPLENYFAFLGSR-EVACFTVVTDGAEKASGPGMILGNFQMQNFYV 430
Query: 1419 EFDLANERFGFRQQLC 1466
E+DL NER GF+++ C
Sbjct: 431 EYDLQNERLGFKKESC 446
>ref|XP_002272243.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
Length = 467
Score = 517 bits (1331), Expect = e-144
Identities = 260/439 (59%), Positives = 322/439 (73%), Gaps = 5/439 (1%)
Frame = +3
Query: 165 ITLPLKLINTNLTSQSQYQKLYYLASLSQARAHHLKTPKPQQQFSNTQLFPRSYGGYSIS 344
ITLPL + Y+ L +L S S RA HLK PK S T LF SYG YSI
Sbjct: 36 ITLPLSASKPS-PPPDPYRNLRHLVSASLIRARHLKNPKTTPT-STTPLFTHSYGAYSIP 93
Query: 345 LNIGTPPQTIPFVMDTGSDFVWFPCTRKYICRNCTFPATQPPP-VFIPKQSSTSKVLGCL 521
L+ GTPPQT+P +MDTGSD VWFPCT +Y+CRNC+F + P +FIPK SS+SKVLGC+
Sbjct: 94 LSFGTPPQTLPLIMDTGSDLVWFPCTHRYVCRNCSFSTSNPSSNIFIPKSSSSSKVLGCV 153
Query: 522 NKKCGWVHRDQDVRTRCSECVNNIKNCSQICPPYIIMYGSGSTGGVSIVDSLNLPGKNVP 701
N KCGW+H + V++RC +C NC+QICPPY++ YGSG TGG+ + ++L+LPGK VP
Sbjct: 154 NPKCGWIHGSK-VQSRCRDCEPTSPNCTQICPPYLVFYGSGITGGIMLSETLDLPGKGVP 212
Query: 702 DFLVGCSVFSSRQPAGIAGFGRGPSSLPNQLGLKKFSYCLLSHKFDDSPESSSLVLYSGK 881
+F+VGCSV S+ QPAGI+GFGRGP SLP+QLGLKKFSYCLLS ++DD+ ESSSLVL
Sbjct: 213 NFIVGCSVLSTSQPAGISGFGRGPPSLPSQLGLKKFSYCLLSRRYDDTTESSSLVLDGES 272
Query: 882 DSDQKTSKLSYTPILKFPEIQDKQAYTTFSGYYYLGLRKILVGGKKIKIPFKYLTPGPTG 1061
DS +KT+ LSYTP ++ P++ K A FS YYYLGLR I VGGK +KIP+KYL PG G
Sbjct: 273 DSGEKTAGLSYTPFVQNPKVAGKHA---FSVYYYLGLRHITVGGKHVKIPYKYLIPGADG 329
Query: 1062 DGGTIVDSGSTFTFLTKNVHELVVSALVEQVKDYKRAKQVESITGLSPCFDISGHRSVNF 1241
DGGTI+DSG+TFT++ + ELV + +QV+ KRA +VE ITGL PCF+ISG + +F
Sbjct: 330 DGGTIIDSGTTFTYMKGEIFELVAAEFEKQVQS-KRATEVEGITGLRPCFNISGLNTPSF 388
Query: 1242 PEMKFHFKGGAEMVLPLVNYFSFVSKELEVVCLTMVTD--AGN--SGGPAIILGNFQLQN 1409
PE+ F+GGAEM LPL NY +F+ + +VVCLT+VTD AG SGGPAIILGNFQ QN
Sbjct: 389 PELTLKFRGGAEMELPLANYVAFLGGD-DVVCLTIVTDGAAGKEFSGGPAIILGNFQQQN 447
Query: 1410 FYTEFDLANERFGFRQQLC 1466
FY E+DL NER GFRQQ C
Sbjct: 448 FYVEYDLRNERLGFRQQSC 466
>ref|XP_002309394.1| predicted protein [Populus trichocarpa] gi|222855370|gb|EEE92917.1|
predicted protein [Populus trichocarpa]
Length = 469
Score = 493 bits (1270), Expect = e-137
Identities = 250/448 (55%), Positives = 318/448 (70%), Gaps = 11/448 (2%)
Frame = +3
Query: 159 ATITLPLKLINTN---LTSQSQYQKLYYLASLSQARAHHLKTPKPQQQFSNTQLFPRSYG 329
+TIT+PL ++ ++S++ + L +LASLS +RAHH+K+PK + T LFPRSYG
Sbjct: 31 STITIPLSAPSSTKLIVSSKNPWGALNHLASLSLSRAHHIKSPKTKFSLLKTPLFPRSYG 90
Query: 330 GYSISLNIGTPPQTIPFVMDTGSDFVWFPCTRKYICRNCTFPATQPP--PVFIPKQSSTS 503
GYSISLN GTPPQT FVMDTGS VWFPCT +Y+C C FP + P FIPKQSS+S
Sbjct: 91 GYSISLNFGTPPQTTKFVMDTGSSLVWFPCTSRYLCSRCDFPNIEVTGIPTFIPKQSSSS 150
Query: 504 KVLGCLNKKCGWVHRDQDVRTRCSECVNNIKNCSQICPPYIIMYGSGSTGGVSIVDSLNL 683
++GC N KC W+ + V+++C EC +NC+Q CPPY+I YG GST G+ + ++L+
Sbjct: 151 NLIGCKNHKCSWLFGPK-VQSKCQECDPTTQNCTQSCPPYVIQYGLGSTAGLLLSETLDF 209
Query: 684 PGKN-VPDFLVGCSVFSSRQPAGIAGFGRGPSSLPNQLGLKKFSYCLLSHKFDDSPESSS 860
P K +P FLVGCS+FS RQP GIAGFGR P SLP+QLGLKKFSYCL+SH FDD+P SS
Sbjct: 210 PHKKTIPGFLVGCSLFSIRQPEGIAGFGRSPESLPSQLGLKKFSYCLVSHAFDDTPASSD 269
Query: 861 LVLYSGKDSDQ-KTSKLSYTPILKFPEIQDKQAYTTFSGYYYLGLRKILVGGKKIKIPFK 1037
LVL +G SD KT LSYTP K P F YYY+ LR I++G +K+P+K
Sbjct: 270 LVLDTGSGSDDTKTPGLSYTPFQKNPT-------AAFRDYYYVLLRNIVIGDTHVKVPYK 322
Query: 1038 YLTPGPTGDGGTIVDSGSTFTFLTKNVHELVVSALVEQVKDYKRAKQVESITGLSPCFDI 1217
+L PG G+GGTIVDSG+TFTF+ K V+ELV +QV Y A +V++ TGL PCF+I
Sbjct: 323 FLVPGSDGNGGTIVDSGTTFTFMEKPVYELVAKEFEKQVAHYTVATEVQNQTGLRPCFNI 382
Query: 1218 SGHRSVNFPEMKFHFKGGAEMVLPLVNYFSFVSKELEVVCLTMVTD----AGNSGGPAII 1385
SG +SV+ PE FHFKGGA+M LPL NYFSFV + V+CLT+V+D +G GGPAII
Sbjct: 383 SGEKSVSVPEFIFHFKGGAKMALPLANYFSFV--DSGVICLTIVSDNMSGSGIGGGPAII 440
Query: 1386 LGNFQLQNFYTEFDLANERFGFRQQLCL 1469
LGN+Q +NF+ EFDL NERFGF+QQ C+
Sbjct: 441 LGNYQQRNFHVEFDLKNERFGFKQQNCV 468
>ref|XP_002312826.1| predicted protein [Populus trichocarpa] gi|222849234|gb|EEE86781.1|
predicted protein [Populus trichocarpa]
Length = 445
Score = 488 bits (1255), Expect = e-135
Identities = 250/453 (55%), Positives = 331/453 (73%), Gaps = 17/453 (3%)
Frame = +3
Query: 159 ATITLPLKLINTN-LTSQSQYQKLYYLASLSQARAHHLKTPKPQQQFSNTQ-LFPRSYGG 332
++IT+PL+ TN + Q QYQKL +L + S ARA HLK P+ + T LF SYGG
Sbjct: 7 SSITIPLQHPQTNQIPFQDQYQKLNHLVTTSLARARHLKNPQTTPATTTTAPLFSHSYGG 66
Query: 333 YSISLNIGTPPQTIPFVMDTGSDFVWFPCTRKYICRNCTFPATQPPP---VFIPKQSSTS 503
YS+SL+ GTPPQT+ F+MDTGSD VWFPCT Y+C++C+F ++ P FIPK+SS+S
Sbjct: 67 YSVSLSFGTPPQTLSFIMDTGSDIVWFPCTSHYLCKHCSFSSSSPSSRIQPFIPKESSSS 126
Query: 504 KVLGCLNKKCGWVHR-----DQDVRTRCSECVNNIKNC-SQICPPYIIMYGSGSTGGVSI 665
K+LGC N KC W+H DQD CS IK+C +Q CPPY+I YGSG+TGGV++
Sbjct: 127 KLLGCKNPKCSWIHHSNINCDQD----CS-----IKSCLNQTCPPYMIFYGSGTTGGVAL 177
Query: 666 VDSLNLPGKNVPDFLVGCSVFSSRQPAGIAGFGRGPSSLPNQLGLKKFSYCLLSHKFDD- 842
++L+L + P+FLVGCSVFSS QPAGIAGFGRG SSLP+QLGL KFSYCLLSH+FDD
Sbjct: 178 SETLHLHSLSKPNFLVGCSVFSSHQPAGIAGFGRGLSSLPSQLGLGKFSYCLLSHRFDDD 237
Query: 843 SPESSSLVLYSGK-DSDQKTSKLSYTPILKFPEIQDKQAYTTFSGYYYLGLRKILVGGKK 1019
+ +SSSLVL + DSD+KT+ L YTP +K P++ +K ++FS YYYLGLR+I VGG
Sbjct: 238 TKKSSSLVLDMEQLDSDKKTNALVYTPFVKNPKVDNK---SSFSVYYYLGLRRITVGGHH 294
Query: 1020 IKIPFKYLTPGPTGDGGTIVDSGSTFTFLTKNVHELVVSALVEQVKDYKRAKQVESITGL 1199
+K+P+KYL+PG G+GG I+DSG+TFTF+ + E + + Q+KDY+R K++E GL
Sbjct: 295 VKVPYKYLSPGEDGNGGVIIDSGTTFTFMAREAFEPLSDEFIRQIKDYRRVKEIEDAIGL 354
Query: 1200 SPCFDISGHRSVNFPEMKFHFKGGAEMVLPLVNYFSFVSKELEVVCLTMVTD--AG--NS 1367
PCF++S ++V+FPE++ +FKGGA++ LP+ NYF+FV EV CLT+VTD AG
Sbjct: 355 RPCFNVSDAKTVSFPELRLYFKGGADVALPVENYFAFVGG--EVACLTVVTDGVAGPERV 412
Query: 1368 GGPAIILGNFQLQNFYTEFDLANERFGFRQQLC 1466
GGP +ILGNFQ+QNFY E+DL NER GF+Q+ C
Sbjct: 413 GGPGMILGNFQMQNFYVEYDLRNERLGFKQEKC 445
>ref|XP_003520712.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Glycine max]
Length = 474
Score = 487 bits (1254), Expect = e-135
Identities = 247/445 (55%), Positives = 307/445 (68%), Gaps = 10/445 (2%)
Frame = +3
Query: 162 TITLPLK--LINTNLTSQSQYQKLYYLASLSQARAHHLKTPKPQQ-QFSNTQLFPRSYGG 332
TITLPL LI + + + L + AS S RAHHLK + T +P+SYGG
Sbjct: 32 TITLPLSPLLIKPHSSDSDPFHSLKFAASASLTRAHHLKHRNNNSPSVATTPAYPKSYGG 91
Query: 333 YSISLNIGTPPQTIPFVMDTGSDFVWFPCTRKYICRNCTFPA--TQPPPVFIPKQSSTSK 506
YSI LN+GTPPQT PFV+DTGS VWFPCT +Y+C +C FP T P FIPK SST+K
Sbjct: 92 YSIDLNLGTPPQTSPFVLDTGSSLVWFPCTSRYLCSHCNFPNIDTTKIPTFIPKNSSTAK 151
Query: 507 VLGCLNKKCGWVHRDQDVRTRCSECVNNIKNCSQICPPYIIMYGSGSTGGVSIVDSLNLP 686
+LGC N KCG++ DV+ RC +C +NCS CP YII YG GST G ++D+LN P
Sbjct: 152 LLGCRNPKCGYIF-GSDVQFRCPQCKPESQNCSLTCPAYIIQYGLGSTAGFLLLDNLNFP 210
Query: 687 GKNVPDFLVGCSVFSSRQPAGIAGFGRGPSSLPNQLGLKKFSYCLLSHKFDDSPESSSLV 866
GK VP FLVGCS+ S RQP+GIAGFGRG SLP+Q+ LK+FSYCL+SH+FDD+P+SS LV
Sbjct: 211 GKTVPQFLVGCSILSIRQPSGIAGFGRGQESLPSQMNLKRFSYCLVSHRFDDTPQSSDLV 270
Query: 867 LYSGKDSDQKTSKLSYTPILKFPEIQDKQAYTTFSGYYYLGLRKILVGGKKIKIPFKYLT 1046
L D KT+ LSYTP P + F YYYL LRK++VGGK +KIP+ +L
Sbjct: 271 LQISSTGDTKTNGLSYTPFRSNPSTNN----PAFKEYYYLTLRKVIVGGKDVKIPYTFLE 326
Query: 1047 PGPTGDGGTIVDSGSTFTFLTKNVHELVVSALVEQV-KDYKRAKQVESITGLSPCFDISG 1223
PG G+GGTIVDSGSTFTF+ + V+ LV V+Q+ K+Y RA+ E+ +GLSPCF+ISG
Sbjct: 327 PGSDGNGGTIVDSGSTFTFMERPVYNLVAQEFVKQLEKNYSRAEDAETQSGLSPCFNISG 386
Query: 1224 HRSVNFPEMKFHFKGGAEMVLPLVNYFSFVSKELEVVCLTMVTDAG----NSGGPAIILG 1391
++V FPE+ F FKGGA+M PL NYFS V + EVVCLT+V+D G + GPAIILG
Sbjct: 387 VKTVTFPELTFKFKGGAKMTQPLQNYFSLVG-DAEVVCLTVVSDGGAGPPKTTGPAIILG 445
Query: 1392 NFQLQNFYTEFDLANERFGFRQQLC 1466
N+Q QNFY E+DL NERFGF + C
Sbjct: 446 NYQQQNFYIEYDLENERFGFGPRSC 470