BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002193
         (1299 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280969.1| PREDICTED: cytochrome P450 716B2 [Vitis vini...   422   e-116
ref|XP_002522937.1| cytochrome P450, putative [Ricinus communis]...   416   e-114
ref|XP_002528002.1| cytochrome P450, putative [Ricinus communis]...   408   e-111
ref|XP_002309057.1| cytochrome P450 [Populus trichocarpa] gi|222...   404   e-110
gb|AFK79029.1| cytochrome P450 CYP716A41 [Bupleurum chinense]         402   e-110

>ref|XP_002280969.1| PREDICTED: cytochrome P450 716B2 [Vitis vinifera]
          Length = 482

 Score =  422 bits (1086), Expect = e-116
 Identities = 199/386 (51%), Positives = 272/386 (70%), Gaps = 2/386 (0%)
 Frame = +1

Query: 148  KHYPPGSLGLPYIGETINFARHLSNGVPEKFIIERQRKYSRKIFKTSLFGETMVFLCSSE 327
            ++ P G+ GLPYIGE+I F  +   G PEKFI ER  K+S K+FKTSLFGET    C S 
Sbjct: 36   RNLPSGNTGLPYIGESIQFLSNGRKGHPEKFISERMLKFSSKVFKTSLFGETAAVFCGSA 95

Query: 328  GNKFVFGNEEKFFRVWFPQSVDKVFTKSGNKSSQDEAIKMRKNMIPFLRADLLRSYVPVM 507
            GNKF+F NE K    W+P SV+K+F  S   SSQ+E+ KMRK +  FL+ + L+ Y+ +M
Sbjct: 96   GNKFLFSNENKLVTAWWPSSVNKIFPSSLQTSSQEESKKMRKLLPGFLKPEALQRYISIM 155

Query: 508  DSFMKEQVNLYWSC-ETLKACPLIKKFTFTLACRLFFSINDPLELEKLSKPFAEIVNGII 684
            D   +      W+  E +   PL K FTF LACRLF S+ DP  +EKL++PF E+  GII
Sbjct: 156  DVIAQRHFESSWNNKEEVTVFPLAKMFTFWLACRLFLSVEDPDHVEKLAEPFNELAAGII 215

Query: 685  SIPIDLPGTRFRGAIKSANIIRHALEVLIRQRKVDIKEKRATLTQDVLSLMLSQADDESE 864
            ++PIDLPGT F   IK++N++R  L  +I++RK+++ + +A+ TQD+LS ML   D+  E
Sbjct: 216  ALPIDLPGTSFNKGIKASNLVRKELHAIIKKRKMNLADNKASTTQDILSHMLLTCDENGE 275

Query: 865  -MSEMDIASDFVAVLMASYDTTNTTLTFVIKYLAENPEIYDQVFKEQMEVAKTKGADELL 1041
             M+E DIA   + +L+  +DT + T+TF++K+LAE P +YD+VFKEQME+AK+K   ELL
Sbjct: 276  YMNEEDIADKILGLLVGGHDTASATITFIVKFLAELPHVYDEVFKEQMEIAKSKAPGELL 335

Query: 1042 NLDDLQKMTYSWNVACEVLRITPPTVGAFREVITDFTYEGYTIPKGWKVYWVPHLTHRDA 1221
            N +D+ KM YSWNVACEV+R+ PP  GAFRE + DF +EG++IPKGWK+YW  H THR+ 
Sbjct: 336  NWEDIPKMRYSWNVACEVMRLAPPVQGAFREAMNDFIFEGFSIPKGWKLYWSTHSTHRNP 395

Query: 1222 EYYKNPEKFDPSRFEGEGVPPYTFVP 1299
            E++  PEKFDPSRF+G+G  PYT+VP
Sbjct: 396  EFFPKPEKFDPSRFDGKGPAPYTYVP 421


>ref|XP_002522937.1| cytochrome P450, putative [Ricinus communis]
            gi|223537831|gb|EEF39448.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 480

 Score =  416 bits (1069), Expect = e-114
 Identities = 202/402 (50%), Positives = 275/402 (68%), Gaps = 10/402 (2%)
 Frame = +1

Query: 124  YNHFISKYKH--------YPPGSLGLPYIGETINFARHLSNGVPEKFIIERQRKYSRKIF 279
            ++ FI  YKH         PPG  GLP++GE++ F      G PEKF+ +R  KYS +IF
Sbjct: 18   FSIFIIFYKHKSQYNYPSLPPGKPGLPFVGESLEFLSSGWKGHPEKFVFDRTSKYSSEIF 77

Query: 280  KTSLFGETMVFLCSSEGNKFVFGNEEKFFRVWFPQSVDKVFTKSGNKSSQDEAIKMRKNM 459
            KT+L G+     C +  NKF+F NE K  + W+P SV+K+F  S   SS++EAIKMRK +
Sbjct: 78   KTNLLGQPAAVFCGASANKFLFSNENKLVQAWWPDSVNKIFPSSLQTSSKEEAIKMRKLL 137

Query: 460  IPFLRADLLRSYVPVMDSFMKEQVNLYWSCET-LKACPLIKKFTFTLACRLFFSINDPLE 636
              F++ + L+ Y+ +MD+  +      W  +  +   PL K +TF LACRLF S+ DP  
Sbjct: 138  PQFMKPEALQRYIGIMDTIAQRHFASGWEKKNEVVVFPLAKNYTFWLACRLFVSLEDPDH 197

Query: 637  LEKLSKPFAEIVNGIISIPIDLPGTRFRGAIKSANIIRHALEVLIRQRKVDIKEKRATLT 816
            + K + PF E+ +GIIS+PIDLPGT FR AIK++N IR  L  +I+QRK+D+ E +A+ T
Sbjct: 198  IAKFADPFQELASGIISVPIDLPGTPFRRAIKASNFIRKELISIIKQRKIDLAEGKASGT 257

Query: 817  QDVLSLMLSQADDESE-MSEMDIASDFVAVLMASYDTTNTTLTFVIKYLAENPEIYDQVF 993
            QD+LS ML  +D++ + M+EMDIA   + +L+  +DT +   TF+IKYLAE P+IYD V+
Sbjct: 258  QDILSHMLLTSDEDGKFMNEMDIADKILGLLIGGHDTASAACTFIIKYLAELPQIYDAVY 317

Query: 994  KEQMEVAKTKGADELLNLDDLQKMTYSWNVACEVLRITPPTVGAFREVITDFTYEGYTIP 1173
            KEQME+AK+KG  ELLN +D+QKM YSWNVACEV+R+ PP  GAFRE I DF + G+ IP
Sbjct: 318  KEQMEIAKSKGEGELLNWEDIQKMKYSWNVACEVMRVAPPLQGAFREAINDFIFNGFYIP 377

Query: 1174 KGWKVYWVPHLTHRDAEYYKNPEKFDPSRFEGEGVPPYTFVP 1299
            KGWK+YW  + TH+ A Y++ PEKFDPSRFEG+G  PYTFVP
Sbjct: 378  KGWKLYWSANSTHKSATYFEEPEKFDPSRFEGKGPAPYTFVP 419


>ref|XP_002528002.1| cytochrome P450, putative [Ricinus communis]
            gi|223532628|gb|EEF34414.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 471

 Score =  408 bits (1049), Expect = e-111
 Identities = 200/388 (51%), Positives = 266/388 (68%), Gaps = 1/388 (0%)
 Frame = +1

Query: 139  SKYKHYPPGSLGLPYIGETINFARHLSNGVPEKFIIERQRKYSRKIFKTSLFGETMVFLC 318
            S  K+ PPG+ G P IGE+I F      G PEKFI +R  K+S K+FKTSL  E     C
Sbjct: 25   SSRKNLPPGNTGWPLIGESIEFLSTGRKGHPEKFIFDRMEKFSSKVFKTSLLLEPAAVFC 84

Query: 319  SSEGNKFVFGNEEKFFRVWFPQSVDKVFTKSGNKSSQDEAIKMRKNMIPFLRADLLRSYV 498
             + GNKF+F NE K    W+P SV+K+F  S   SSQ+E+ +MRK +  FL+ + L+ Y+
Sbjct: 85   GAAGNKFLFSNENKLVTAWWPNSVNKIFPSSLQTSSQEESKRMRKLLPQFLKPEALQRYI 144

Query: 499  PVMDSFMKEQVNLYWSC-ETLKACPLIKKFTFTLACRLFFSINDPLELEKLSKPFAEIVN 675
             +MD   +      W+  + +   PL K +TF LACRLF S+ D  E+EK +KPF  + +
Sbjct: 145  SIMDVIAQRHFAFGWNNKQQVTVFPLAKMYTFWLACRLFLSMEDREEVEKFAKPFDVLAS 204

Query: 676  GIISIPIDLPGTRFRGAIKSANIIRHALEVLIRQRKVDIKEKRATLTQDVLSLMLSQADD 855
            GIISIPID PGT F   IK++N +R  L  +I QRK+D+ E +A+ TQD+LS ML+ AD+
Sbjct: 205  GIISIPIDFPGTPFNRGIKASNEVRRELIKMIEQRKIDLAENKASPTQDILSHMLTTADE 264

Query: 856  ESEMSEMDIASDFVAVLMASYDTTNTTLTFVIKYLAENPEIYDQVFKEQMEVAKTKGADE 1035
               M+EMDIA   + +L+  +DT +  +TFV+KYLAE P++Y++V +EQME+AK K A E
Sbjct: 265  Y--MNEMDIADKILGLLIGGHDTASAAITFVVKYLAEMPQVYNKVLEEQMEIAKAKAAGE 322

Query: 1036 LLNLDDLQKMTYSWNVACEVLRITPPTVGAFREVITDFTYEGYTIPKGWKVYWVPHLTHR 1215
            LLN +D+QKM YSWNVACEV+R+ PP  GAFRE +TDFTY G+TIPKGWK+YW  + THR
Sbjct: 323  LLNWEDIQKMRYSWNVACEVMRLAPPLQGAFREAMTDFTYAGFTIPKGWKLYWGANSTHR 382

Query: 1216 DAEYYKNPEKFDPSRFEGEGVPPYTFVP 1299
            + E +  PEKFDPSRFEG+G  PYTFVP
Sbjct: 383  NPECFPEPEKFDPSRFEGKGPAPYTFVP 410


>ref|XP_002309057.1| cytochrome P450 [Populus trichocarpa] gi|222855033|gb|EEE92580.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 484

 Score =  404 bits (1039), Expect = e-110
 Identities = 191/396 (48%), Positives = 268/396 (67%), Gaps = 2/396 (0%)
 Frame = +1

Query: 118  NIYNHFISKYKHYPPGSLGLPYIGETINFARHLSNGVPEKFIIERQRKYSRKIFKTSLFG 297
            N + +++S   + PPG+ GLP IGE++ F      G PEKFI++R  K+S K+FKTSLF 
Sbjct: 27   NKHKYYLSSLNNLPPGNTGLPLIGESLEFLTTGQKGQPEKFILDRMAKFSSKVFKTSLFC 86

Query: 298  ETMVFLCSSEGNKFVFGNEEKFFRVWFPQSVDKVFTKSGNKSSQDEAIKMRKNMIPFLRA 477
            E     C + GNKF+F NE K    W+P SV+K+F  S   SSQ+E+ KMRK    F + 
Sbjct: 87   EPTAVFCGAAGNKFLFSNENKLVTAWWPDSVNKIFPSSQQTSSQEESKKMRKLFPLFFKP 146

Query: 478  DLLRSYVPVMDSFMKEQVNLYWSC-ETLKACPLIKKFTFTLACRLFFSINDPLELEKLSK 654
            + L+ Y+ VMD   +  +   W   + +   PL K +TF LACRLF S+ DP E++K +K
Sbjct: 147  ESLQRYISVMDVIAQRHLASDWEGKQEVSVFPLAKTYTFWLACRLFLSMEDPEEVQKFAK 206

Query: 655  PFAEIVNGIISIPIDLPGTRFRGAIKSANIIRHALEVLIRQRKVDIKEKRATLTQDVLSL 834
            PF ++  GIISIPIDLP T F   +K++N++   L  +I+QRK+D+ E +A+ TQD+LS 
Sbjct: 207  PFNDLAAGIISIPIDLPWTPFNRGVKASNVVHKELLKIIKQRKIDLAENKASPTQDILSH 266

Query: 835  MLSQADDESE-MSEMDIASDFVAVLMASYDTTNTTLTFVIKYLAENPEIYDQVFKEQMEV 1011
            ML+ ADD  + M ++DIA   + +L+  +DT +  +TF++KYLAE P +Y+++ +EQ E+
Sbjct: 267  MLTTADDNGQCMKKIDIADKILGLLVGGHDTASAAITFIVKYLAELPHVYNKLLEEQREI 326

Query: 1012 AKTKGADELLNLDDLQKMTYSWNVACEVLRITPPTVGAFREVITDFTYEGYTIPKGWKVY 1191
            AKTK   ELLN +D+Q+M YSWNVACEV+R+ PP  GAFRE +T+F Y G+TIPKGWK+Y
Sbjct: 327  AKTKTPGELLNWEDIQRMRYSWNVACEVMRVAPPLQGAFREAMTEFNYAGFTIPKGWKLY 386

Query: 1192 WVPHLTHRDAEYYKNPEKFDPSRFEGEGVPPYTFVP 1299
            W  + TH++ E +  PE FDPSRFEG G  PYTFVP
Sbjct: 387  WSANTTHKNPECFPEPENFDPSRFEGNGPAPYTFVP 422


>gb|AFK79029.1| cytochrome P450 CYP716A41 [Bupleurum chinense]
          Length = 482

 Score =  402 bits (1034), Expect = e-110
 Identities = 194/387 (50%), Positives = 266/387 (68%), Gaps = 2/387 (0%)
 Frame = +1

Query: 145  YKHYPPGSLGLPYIGETINFARHLSNGVPEKFIIERQRKYSRKIFKTSLFGETMVFLCSS 324
            YK  PPG  G   IGE++ F      G PEKFI +R  KY+ KIFK+SLFGE  +  C +
Sbjct: 35   YKSLPPGETGYFLIGESLEFLSTGRKGHPEKFIFDRMTKYASKIFKSSLFGEKTIVFCGA 94

Query: 325  EGNKFVFGNEEKFFRVWFPQSVDKVFTKSGNKSSQDEAIKMRKNMIPFLRADLLRSYVPV 504
              NKF+F +E K  + W+P SV+K+F  S   SS++EAIKMRK +  F + + L+ YV V
Sbjct: 95   ANNKFLFSDENKLVQSWWPNSVNKLFPSSTQTSSKEEAIKMRKMLPNFFKPEALQRYVGV 154

Query: 505  MDSFMKEQVNLYWSCE-TLKACPLIKKFTFTLACRLFFSINDPLELEKLSKPFAEIVNGI 681
            MD   ++  +  W  + T+   PL K+FTF LACRLF S+ DP ++ K ++PF  + +G+
Sbjct: 155  MDEIAQKHFDSCWENKHTVIVAPLTKRFTFWLACRLFVSLEDPTQVAKFAEPFNLLASGV 214

Query: 682  ISIPIDLPGTRFRGAIKSANIIRHALEVLIRQRKVDIKEKRATLTQDVLSLMLSQADDES 861
             SIPIDLPGT F  AIK++N IR  L  +I++RKVD+++  A+ TQD+LS ML  +D+  
Sbjct: 215  FSIPIDLPGTAFNRAIKASNFIRKTLIGIIKKRKVDLEDGTASATQDILSHMLLTSDETG 274

Query: 862  E-MSEMDIASDFVAVLMASYDTTNTTLTFVIKYLAENPEIYDQVFKEQMEVAKTKGADEL 1038
            + M+E DIA   + +L+  +DT ++    ++KYLAE P IYD V++EQME+AK+K   EL
Sbjct: 275  KFMTEADIADKILGLLIGGHDTASSACALIVKYLAELPHIYDGVYREQMEIAKSKSPGEL 334

Query: 1039 LNLDDLQKMTYSWNVACEVLRITPPTVGAFREVITDFTYEGYTIPKGWKVYWVPHLTHRD 1218
            LN DD+QKM YSWNVACEVLR+ PP  G+FREV++DF + G++IPKGWK+YW  + TH+ 
Sbjct: 335  LNWDDVQKMKYSWNVACEVLRLAPPLQGSFREVLSDFMHNGFSIPKGWKIYWSANSTHKS 394

Query: 1219 AEYYKNPEKFDPSRFEGEGVPPYTFVP 1299
            +EY+  PEKFDP RFEG G  PYTFVP
Sbjct: 395  SEYFPEPEKFDPRRFEGSGPAPYTFVP 421