BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002052
         (1625 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEO86798.1| methionine synthase [Camellia sinensis]                905   0.0  
gb|ABI35986.1| methionine synthase [Orobanche ramosa]                 903   0.0  
ref|XP_002325856.1| vitamin-b12 independent methionine synthase,...   901   0.0  
ref|XP_004141126.1| PREDICTED: 5-methyltetrahydropteroyltrigluta...   899   0.0  
ref|XP_002328083.1| vitamin-b12 independent methionine synthase,...   899   0.0  

>gb|AEO86798.1| methionine synthase [Camellia sinensis]
          Length = 765

 Score =  905 bits (2339), Expect = 0.0
 Identities = 448/541 (82%), Positives = 500/541 (92%)
 Frame = -1

Query: 1625 DTNYHFIVPELGPDVKFSYASHKAVHEYKEAKALGVETVPVLVGPISYLLLSKPEKGVEK 1446
            DTNYHFIVPELGP++KFSYASHKAV EYKEAKALGV+TVPVLVGP+SYLLLSKP KGVEK
Sbjct: 114  DTNYHFIVPELGPEIKFSYASHKAVDEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEK 173

Query: 1445 SFSPLSLVNEILPIYKEVVSELKEAGATWVQFDEPTLVMDLDSDELQAFSLAYTELESSL 1266
            SFS LSL+++ILPIYKEVV+ELK AGA+W+QFDEPTLV DL+S +LQAF+ AY+ELES+L
Sbjct: 174  SFSLLSLLDKILPIYKEVVAELKAAGASWIQFDEPTLVKDLESHQLQAFTAAYSELESAL 233

Query: 1265 SGLNVLVESYFGDLPIESYKTITSLKAITGFGFDLVRGSKTIDLVKCGFPSDRYLFAGVV 1086
            SGLNV+VE+YF D+P E++KT+TSLK +TGFGFDLVRG++T+DL+  GFPS  YLFAGVV
Sbjct: 234  SGLNVIVETYFADVPAEAFKTLTSLKCVTGFGFDLVRGAQTLDLITSGFPSGEYLFAGVV 293

Query: 1085 DGRNIWANDLNASLSSLQYLKSLVGKDKLVVSTSCSLLHTAVDKISETELDEEFKSWLAF 906
            DGRNIWANDL ASLS LQ L+ +VGKDKLVVSTSCSLLHTAVD ++ET+ D+E KSWLAF
Sbjct: 294  DGRNIWANDLAASLSLLQTLEGIVGKDKLVVSTSCSLLHTAVDLVNETKFDKELKSWLAF 353

Query: 905  AAQKVVEVNALAEALTGNKDEAFFTANSAALASRMSSQRVTDEAVQKAVATLKGSDHRRG 726
            AAQKVVEVN+LA+AL G+KDEAFF+ N+AA ASR SS RVT+EAVQKA A L+GSDHRR 
Sbjct: 354  AAQKVVEVNSLAKALAGHKDEAFFSENAAAQASRKSSPRVTNEAVQKAAAALRGSDHRRA 413

Query: 725  TSVGARLEAQQLKLNLPILPTTTIGSFPQTMELRKVRREYKANKISEEVYVNIIKDEINK 546
            T+V ARL+AQQ KLNLPILPTTTIGSFPQT+ELR+VRREYKA KISEE YV  IK+EINK
Sbjct: 414  TNVSARLDAQQKKLNLPILPTTTIGSFPQTIELRRVRREYKAKKISEEEYVKAIKEEINK 473

Query: 545  VVKLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTLNGWVQSYGSRCVKPPIIYGDVS 366
            VVKLQEEL IDVLVHGEPERNDMVEYFGEQLSGFAF+ NGWVQSYGSRCVKPPII+GDVS
Sbjct: 474  VVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIFGDVS 533

Query: 365  RPKAMTVFWSSIAQSMTKRPMKGMLTGPVTILNWSFVRDDQPRFETCYQIALAIKNEVED 186
            RPKAMTVFWSS+AQSMT RPMKGMLTGPVTILNWSFVR+DQPRFETCYQIALAIK+EVED
Sbjct: 534  RPKAMTVFWSSMAQSMTTRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVED 593

Query: 185  LEKAGIDVIQIDEAALREGLPLRKAEQAFYLDWAVQSFRITNAGVKDTTQIHTHMCYSNF 6
            LEKAGI+VIQ+DEAALREGLPLRK+EQAFYLDWAV SFRITN GV DTTQIHTHMCYSNF
Sbjct: 594  LEKAGINVIQMDEAALREGLPLRKSEQAFYLDWAVHSFRITNCGVTDTTQIHTHMCYSNF 653

Query: 5    N 3
            N
Sbjct: 654  N 654


>gb|ABI35986.1| methionine synthase [Orobanche ramosa]
          Length = 765

 Score =  903 bits (2333), Expect = 0.0
 Identities = 445/541 (82%), Positives = 497/541 (91%)
 Frame = -1

Query: 1625 DTNYHFIVPELGPDVKFSYASHKAVHEYKEAKALGVETVPVLVGPISYLLLSKPEKGVEK 1446
            DTNYHFIVPELGPDVKFSYASHKAV+EY EAKALGV TVPVLVGP++YLLLSKP KGVEK
Sbjct: 114  DTNYHFIVPELGPDVKFSYASHKAVNEYNEAKALGVNTVPVLVGPVTYLLLSKPAKGVEK 173

Query: 1445 SFSPLSLVNEILPIYKEVVSELKEAGATWVQFDEPTLVMDLDSDELQAFSLAYTELESSL 1266
            +F  LSL+++ILPIYKEV++ELK AGA+W+QFDEPTLV+DL+S +L+AF+ AY ELESSL
Sbjct: 174  TFPLLSLLDKILPIYKEVIAELKAAGASWIQFDEPTLVLDLESHQLEAFTKAYAELESSL 233

Query: 1265 SGLNVLVESYFGDLPIESYKTITSLKAITGFGFDLVRGSKTIDLVKCGFPSDRYLFAGVV 1086
            SGLNVL+E+YF D+P  +YK +TSL A++GFGFDLVRG++T+DL+K GFPS +YLFAGVV
Sbjct: 234  SGLNVLIETYFADVPAAAYKALTSLSAVSGFGFDLVRGTQTLDLIKGGFPSGKYLFAGVV 293

Query: 1085 DGRNIWANDLNASLSSLQYLKSLVGKDKLVVSTSCSLLHTAVDKISETELDEEFKSWLAF 906
            DGRNIWANDL+ASL +LQ L+ ++GKDKLVVSTSCSLLHTAVD ++E +LD+E KSWLAF
Sbjct: 294  DGRNIWANDLSASLCTLQSLEGIIGKDKLVVSTSCSLLHTAVDLVNEPKLDQEIKSWLAF 353

Query: 905  AAQKVVEVNALAEALTGNKDEAFFTANSAALASRMSSQRVTDEAVQKAVATLKGSDHRRG 726
            AAQKVVEVNALA+AL G KDEAFF+AN+AA ASR SS RVT+EAVQKA A LKGSDHRR 
Sbjct: 354  AAQKVVEVNALAKALAGEKDEAFFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRA 413

Query: 725  TSVGARLEAQQLKLNLPILPTTTIGSFPQTMELRKVRREYKANKISEEVYVNIIKDEINK 546
            T+V ARL+AQQ KLNLPILPTTTIGSFPQT+ELR+VRREYKA KISEE Y+  IK+EINK
Sbjct: 414  TNVSARLDAQQKKLNLPILPTTTIGSFPQTIELRRVRREYKAKKISEEEYIKAIKEEINK 473

Query: 545  VVKLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTLNGWVQSYGSRCVKPPIIYGDVS 366
            VVKLQEEL IDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVS
Sbjct: 474  VVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVS 533

Query: 365  RPKAMTVFWSSIAQSMTKRPMKGMLTGPVTILNWSFVRDDQPRFETCYQIALAIKNEVED 186
            RPK MTVFWS+ AQSMTKRPMKGMLTGPVTILNWSFVR+DQPRFETCYQIALAIK+EVED
Sbjct: 534  RPKPMTVFWSTAAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVED 593

Query: 185  LEKAGIDVIQIDEAALREGLPLRKAEQAFYLDWAVQSFRITNAGVKDTTQIHTHMCYSNF 6
            LEKAGI VIQIDEAALREGLPLRK+E AFYLDWAV SFRITN GV+DTTQIHTHMCYSNF
Sbjct: 594  LEKAGITVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVEDTTQIHTHMCYSNF 653

Query: 5    N 3
            N
Sbjct: 654  N 654


>ref|XP_002325856.1| vitamin-b12 independent methionine synthase,
            5-methyltetrahydropteroyltriglutamate-homocysteine
            [Populus trichocarpa] gi|222862731|gb|EEF00238.1|
            vitamin-b12 independent methionine synthase,
            5-methyltetrahydropteroyltriglutamate-homocysteine
            [Populus trichocarpa]
          Length = 765

 Score =  901 bits (2328), Expect = 0.0
 Identities = 446/541 (82%), Positives = 494/541 (91%)
 Frame = -1

Query: 1625 DTNYHFIVPELGPDVKFSYASHKAVHEYKEAKALGVETVPVLVGPISYLLLSKPEKGVEK 1446
            DTNYHFIVPELGPDVKFSYASHKAV EYKEAKALGV+TVPVL+GP+SYLLLSKP KGVEK
Sbjct: 114  DTNYHFIVPELGPDVKFSYASHKAVTEYKEAKALGVDTVPVLIGPVSYLLLSKPAKGVEK 173

Query: 1445 SFSPLSLVNEILPIYKEVVSELKEAGATWVQFDEPTLVMDLDSDELQAFSLAYTELESSL 1266
            SFS LSL+ +ILPIYKEV+SELKEAGA+W+QFDEPTLVMDL+S +LQAF+ AY+ LES+L
Sbjct: 174  SFSLLSLLGKILPIYKEVISELKEAGASWIQFDEPTLVMDLESHKLQAFTGAYSALESTL 233

Query: 1265 SGLNVLVESYFGDLPIESYKTITSLKAITGFGFDLVRGSKTIDLVKCGFPSDRYLFAGVV 1086
            SGLNVL+E+YF D+P E YKT+TSLK +T FGFDLVRG+KT+DL+K  FP  +YLFAGVV
Sbjct: 234  SGLNVLIETYFADIPAEQYKTLTSLKGVTAFGFDLVRGTKTLDLIKGDFPKGKYLFAGVV 293

Query: 1085 DGRNIWANDLNASLSSLQYLKSLVGKDKLVVSTSCSLLHTAVDKISETELDEEFKSWLAF 906
            DGRNIWANDL AS S+L+ L+ +VGKDKLVVSTSCSLLHTAVD ++ET+LD+E KSWLAF
Sbjct: 294  DGRNIWANDLAASFSTLETLEGIVGKDKLVVSTSCSLLHTAVDLVNETKLDKEIKSWLAF 353

Query: 905  AAQKVVEVNALAEALTGNKDEAFFTANSAALASRMSSQRVTDEAVQKAVATLKGSDHRRG 726
            AAQKVVEVNALA+AL G KDE  F+AN+AALASR SS RV +EAVQKA A LKGSDHRR 
Sbjct: 354  AAQKVVEVNALAKALAGQKDEEIFSANAAALASRKSSPRVNNEAVQKAAAALKGSDHRRA 413

Query: 725  TSVGARLEAQQLKLNLPILPTTTIGSFPQTMELRKVRREYKANKISEEVYVNIIKDEINK 546
            T+V ARL+AQQ KLNLPILPTTTIGSFPQTMELRKVRREYKA K+SE+ YV  IK+EI K
Sbjct: 414  TNVSARLDAQQKKLNLPILPTTTIGSFPQTMELRKVRREYKAKKVSEDDYVKAIKEEIRK 473

Query: 545  VVKLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTLNGWVQSYGSRCVKPPIIYGDVS 366
            VVKLQEE  IDVLVHGEPERNDMVEYFGEQLSGFAF+ NGWVQSYGSRCVKPPIIYGDVS
Sbjct: 474  VVKLQEEFDIDVLVHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIYGDVS 533

Query: 365  RPKAMTVFWSSIAQSMTKRPMKGMLTGPVTILNWSFVRDDQPRFETCYQIALAIKNEVED 186
            RPK+MTVFWS++AQSMT RPMKGMLTGPVTILNWSFVR+DQPR+ETCYQIALAIK+EVED
Sbjct: 534  RPKSMTVFWSTLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYETCYQIALAIKDEVED 593

Query: 185  LEKAGIDVIQIDEAALREGLPLRKAEQAFYLDWAVQSFRITNAGVKDTTQIHTHMCYSNF 6
            LEK GI VIQIDEAALREGLPLRK+EQAFYLDWAV SFRITN GV+DTTQIHTHMCYSNF
Sbjct: 594  LEKNGITVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNF 653

Query: 5    N 3
            N
Sbjct: 654  N 654


>ref|XP_004141126.1| PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine
            methyltransferase-like [Cucumis sativus]
            gi|449489509|ref|XP_004158333.1| PREDICTED:
            5-methyltetrahydropteroyltriglutamate--homocysteine
            methyltransferase-like [Cucumis sativus]
          Length = 765

 Score =  899 bits (2324), Expect = 0.0
 Identities = 445/541 (82%), Positives = 495/541 (91%)
 Frame = -1

Query: 1625 DTNYHFIVPELGPDVKFSYASHKAVHEYKEAKALGVETVPVLVGPISYLLLSKPEKGVEK 1446
            DTNYHFIVPELGP+VKFSYASHKAV EYKEAKALGVETVPVL+GP+SYLLLSKP KGV+K
Sbjct: 114  DTNYHFIVPELGPEVKFSYASHKAVEEYKEAKALGVETVPVLIGPVSYLLLSKPAKGVDK 173

Query: 1445 SFSPLSLVNEILPIYKEVVSELKEAGATWVQFDEPTLVMDLDSDELQAFSLAYTELESSL 1266
            SFS LSL+++ILPIYKEVVS+LK AGA+W+QFDEPTLV DLDSD+L+AFS AY +LES+L
Sbjct: 174  SFSLLSLLDKILPIYKEVVSDLKAAGASWIQFDEPTLVKDLDSDKLKAFSDAYAQLESTL 233

Query: 1265 SGLNVLVESYFGDLPIESYKTITSLKAITGFGFDLVRGSKTIDLVKCGFPSDRYLFAGVV 1086
            SGLNVLVE+YF D+P E+YKT+TSLK +T +GFDLVRG+KT+DL+K  FP  ++LFAGVV
Sbjct: 234  SGLNVLVETYFADIPAEAYKTLTSLKGVTAYGFDLVRGTKTLDLIKGDFPKGKFLFAGVV 293

Query: 1085 DGRNIWANDLNASLSSLQYLKSLVGKDKLVVSTSCSLLHTAVDKISETELDEEFKSWLAF 906
            DGRNIWANDL AS+S L+ L  +VGKD LVVSTSCSLLHTAVD ++ET+LD E KSWLAF
Sbjct: 294  DGRNIWANDLAASVSVLEELAGIVGKDHLVVSTSCSLLHTAVDLVNETKLDNEIKSWLAF 353

Query: 905  AAQKVVEVNALAEALTGNKDEAFFTANSAALASRMSSQRVTDEAVQKAVATLKGSDHRRG 726
            AAQK++EVNALA+AL GNKD AFF +N+ A ASR +S RVT+EAVQKA A LKGSDHRR 
Sbjct: 354  AAQKIIEVNALAKALAGNKDGAFFASNAGAHASRKASPRVTNEAVQKAAAALKGSDHRRA 413

Query: 725  TSVGARLEAQQLKLNLPILPTTTIGSFPQTMELRKVRREYKANKISEEVYVNIIKDEINK 546
            T+V ARL+AQQ KLNLPILPTTTIGSFPQT+ELR+VRREYKANKISE+ YV  IK+EINK
Sbjct: 414  TNVSARLDAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKANKISEDEYVKAIKEEINK 473

Query: 545  VVKLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTLNGWVQSYGSRCVKPPIIYGDVS 366
            VVKLQEEL IDVLVHGEPERNDMVEYFGEQLSGFAFT+NGWVQSYGSRCVKPPIIYGDVS
Sbjct: 474  VVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVS 533

Query: 365  RPKAMTVFWSSIAQSMTKRPMKGMLTGPVTILNWSFVRDDQPRFETCYQIALAIKNEVED 186
            RPK MTVFWS+ AQSMT RPMKGMLTGPVTILNWSFVR DQPRFETCYQIALAIK+EVED
Sbjct: 534  RPKPMTVFWSTTAQSMTSRPMKGMLTGPVTILNWSFVRVDQPRFETCYQIALAIKDEVED 593

Query: 185  LEKAGIDVIQIDEAALREGLPLRKAEQAFYLDWAVQSFRITNAGVKDTTQIHTHMCYSNF 6
            LEKAGI+VIQIDEAALREGLPLRK+EQAFYLDWAV SFRITN GV+DTTQIHTHMCYSNF
Sbjct: 594  LEKAGINVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNF 653

Query: 5    N 3
            N
Sbjct: 654  N 654


>ref|XP_002328083.1| vitamin-b12 independent methionine synthase,
            5-methyltetrahydropteroyltriglutamate-homocysteine
            [Populus trichocarpa] gi|222837598|gb|EEE75963.1|
            vitamin-b12 independent methionine synthase,
            5-methyltetrahydropteroyltriglutamate-homocysteine
            [Populus trichocarpa]
          Length = 765

 Score =  899 bits (2324), Expect = 0.0
 Identities = 443/541 (81%), Positives = 500/541 (92%)
 Frame = -1

Query: 1625 DTNYHFIVPELGPDVKFSYASHKAVHEYKEAKALGVETVPVLVGPISYLLLSKPEKGVEK 1446
            DTNYH+IVPELGP+V FSYASHKAV+EYKEAKALGV+TVPVLVGP+SYLLLSKP KGVEK
Sbjct: 114  DTNYHYIVPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEK 173

Query: 1445 SFSPLSLVNEILPIYKEVVSELKEAGATWVQFDEPTLVMDLDSDELQAFSLAYTELESSL 1266
            SFS LSL+++ILP+Y+EV++ELK AGA+W+QFDEP LVMDL + ELQAF+ AY+ LE+SL
Sbjct: 174  SFSLLSLIDKILPVYQEVLAELKAAGASWIQFDEPKLVMDLGAHELQAFTHAYSALEASL 233

Query: 1265 SGLNVLVESYFGDLPIESYKTITSLKAITGFGFDLVRGSKTIDLVKCGFPSDRYLFAGVV 1086
            SGLNVLVE+YF D+P+E+YKT+TSLK++TGFGFDLVRG+KT++L+K GFPS +YLFAGVV
Sbjct: 234  SGLNVLVETYFADVPVEAYKTLTSLKSVTGFGFDLVRGTKTLELIKGGFPSGKYLFAGVV 293

Query: 1085 DGRNIWANDLNASLSSLQYLKSLVGKDKLVVSTSCSLLHTAVDKISETELDEEFKSWLAF 906
            DGRNIWAN+L +SL +L+ L+ +VGKDKLVVSTSCSLLHTAVD ++E +LD E KSWLAF
Sbjct: 294  DGRNIWANNLGSSLDTLKALEGIVGKDKLVVSTSCSLLHTAVDLVNEPKLDREIKSWLAF 353

Query: 905  AAQKVVEVNALAEALTGNKDEAFFTANSAALASRMSSQRVTDEAVQKAVATLKGSDHRRG 726
            AAQKVVEVNALA+AL G +DEAFF+AN+AA ASR SS RVT+EAVQKA   LKGSDHRR 
Sbjct: 354  AAQKVVEVNALAKALAGQQDEAFFSANAAAQASRKSSPRVTNEAVQKAAVALKGSDHRRA 413

Query: 725  TSVGARLEAQQLKLNLPILPTTTIGSFPQTMELRKVRREYKANKISEEVYVNIIKDEINK 546
            T+V ARL+AQQ KLNLPILPTTTIGSFPQTM+LR+VRREYKA KISE+ YV  IK+EINK
Sbjct: 414  TNVSARLDAQQKKLNLPILPTTTIGSFPQTMDLRRVRREYKAKKISEQNYVEAIKEEINK 473

Query: 545  VVKLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTLNGWVQSYGSRCVKPPIIYGDVS 366
            VVK+QEEL IDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVS
Sbjct: 474  VVKIQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVS 533

Query: 365  RPKAMTVFWSSIAQSMTKRPMKGMLTGPVTILNWSFVRDDQPRFETCYQIALAIKNEVED 186
            RPKAMTVFWSS+AQSMTKRPMKGMLTGPVTILNWSFVR+DQPRFETCYQIALAIK+EVED
Sbjct: 534  RPKAMTVFWSSMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVED 593

Query: 185  LEKAGIDVIQIDEAALREGLPLRKAEQAFYLDWAVQSFRITNAGVKDTTQIHTHMCYSNF 6
            LEKAGI VIQIDEAALREGLPLRK+E AFYL+WAV SFRITN GV+DTTQIHTHMCYSNF
Sbjct: 594  LEKAGITVIQIDEAALREGLPLRKSEHAFYLNWAVHSFRITNCGVEDTTQIHTHMCYSNF 653

Query: 5    N 3
            N
Sbjct: 654  N 654