BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002044
         (1115 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAJ51944.1| NADP-dependent malic enzyme [Nicotiana benthamiana]   158   e-116
gb|AAK83074.1|AF288921_1 putative cytosolic NADP-malic enzyme [F...   158   e-115
gb|AAK83073.1|AF288920_1 putative cytosolic NADP-malic enzyme [F...   158   e-114
ref|XP_003547744.1| PREDICTED: NADP-dependent malic enzyme-like ...   158   e-113
ref|XP_002324450.1| predicted protein [Populus trichocarpa] gi|2...   159   e-113

>dbj|BAJ51944.1| NADP-dependent malic enzyme [Nicotiana benthamiana]
          Length = 532

 Score =  158 bits (400), Expect(3) = e-116
 Identities = 81/131 (61%), Positives = 98/131 (74%), Gaps = 4/131 (3%)
 Frame = -2

Query: 1054 DSTLKDMK-NGELE---SNNSGVAGGVEDMYGEDRATQDQPVTPWAISVASGHTXXXXXX 887
            +STLK++  NG+      +  GVAGGVEDMYGEDRAT+DQP+TPW  SVASG++      
Sbjct: 2    ESTLKEISSNGDSVLDMKDKCGVAGGVEDMYGEDRATEDQPITPWTFSVASGYSL----- 56

Query: 886  XXXXXXXXXMRDPRHNKGTAFTEKERDAHYLRGLLPPVVLSQELQEKKMIQNIRSYEVPL 707
                     +RDP +NKG AFTE+ERDAHYLRGLLPP VLSQELQEK++I N+R Y+VPL
Sbjct: 57   ---------LRDPHYNKGLAFTEEERDAHYLRGLLPPTVLSQELQEKRLIHNLRQYQVPL 107

Query: 706  QKYTAMMDLQE 674
            Q+Y AMMDLQE
Sbjct: 108  QRYMAMMDLQE 118



 Score =  156 bits (394), Expect(3) = e-116
 Identities = 74/80 (92%), Positives = 79/80 (98%)
 Frame = -3

Query: 561 NVEELLPIVYTPVVGEACQKYGSIFKRPQGLYISLKEKGRILEVLKNWPEKSIQVIVVTD 382
           +VEELLPIVYTP VGEACQKYGSIF+RPQGL+ISLKEKG+ILEVLKNWPEK+IQVIVVTD
Sbjct: 131 HVEELLPIVYTPTVGEACQKYGSIFRRPQGLFISLKEKGKILEVLKNWPEKTIQVIVVTD 190

Query: 381 GERILGLGDLGCQGMGIPVG 322
           GERILGLGDLGCQGMGIPVG
Sbjct: 191 GERILGLGDLGCQGMGIPVG 210



 Score =  153 bits (386), Expect(3) = e-116
 Identities = 74/78 (94%), Positives = 78/78 (100%)
 Frame = -2

Query: 235 GKLALYTALGGVRPSVCLPITIDVGTNNQKLLDDEFYIGLKQKRATGQEYAELLEEFMTA 56
           GKL+LYTALGGVRPSVCLPITIDVGTNNQKLLD+EFYIGLKQKRATGQEYAELLEEFM+A
Sbjct: 210 GKLSLYTALGGVRPSVCLPITIDVGTNNQKLLDNEFYIGLKQKRATGQEYAELLEEFMSA 269

Query: 55  VKQNYGEKILIQFEDFAN 2
           VKQNYGEK+LIQFEDFAN
Sbjct: 270 VKQNYGEKVLIQFEDFAN 287


>gb|AAK83074.1|AF288921_1 putative cytosolic NADP-malic enzyme [Flaveria pringlei]
          Length = 589

 Score =  158 bits (399), Expect(3) = e-115
 Identities = 74/80 (92%), Positives = 79/80 (98%)
 Frame = -3

Query: 561 NVEELLPIVYTPVVGEACQKYGSIFKRPQGLYISLKEKGRILEVLKNWPEKSIQVIVVTD 382
           NVEELLP+VYTP VGEACQKYGSIFKRPQGLYISLKEKG+ILEVL+NWPE++IQVIVVTD
Sbjct: 128 NVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKILEVLRNWPERNIQVIVVTD 187

Query: 381 GERILGLGDLGCQGMGIPVG 322
           GERILGLGDLGCQGMGIPVG
Sbjct: 188 GERILGLGDLGCQGMGIPVG 207



 Score =  157 bits (397), Expect(3) = e-115
 Identities = 77/127 (60%), Positives = 97/127 (76%), Gaps = 1/127 (0%)
 Frame = -2

Query: 1051 STLKDMKNGE-LESNNSGVAGGVEDMYGEDRATQDQPVTPWAISVASGHTXXXXXXXXXX 875
            STLK MK GE +  ++S V GGV+D+YGEDRA++DQ +TPW +SVASG+T          
Sbjct: 3    STLKQMKEGESVVDHSSAVGGGVDDVYGEDRASEDQLITPWTVSVASGYTL--------- 53

Query: 874  XXXXXMRDPRHNKGTAFTEKERDAHYLRGLLPPVVLSQELQEKKMIQNIRSYEVPLQKYT 695
                 +RDP HNKG AFT++ERD+HYLRGLLPP V +QELQEKK++QNIRSYEVPL +Y 
Sbjct: 54   -----LRDPHHNKGLAFTQRERDSHYLRGLLPPAVATQELQEKKLMQNIRSYEVPLHRYV 108

Query: 694  AMMDLQE 674
            AMM+L+E
Sbjct: 109  AMMELEE 115



 Score =  148 bits (373), Expect(3) = e-115
 Identities = 70/78 (89%), Positives = 76/78 (97%)
 Frame = -2

Query: 235 GKLALYTALGGVRPSVCLPITIDVGTNNQKLLDDEFYIGLKQKRATGQEYAELLEEFMTA 56
           GKLALYTALGGVRPS CLPITIDVGTNNQKLLDDEFYIGL+QKRATG+EY +LLEEFM+A
Sbjct: 207 GKLALYTALGGVRPSACLPITIDVGTNNQKLLDDEFYIGLRQKRATGKEYYDLLEEFMSA 266

Query: 55  VKQNYGEKILIQFEDFAN 2
           VKQNYGEK+L+QFEDFAN
Sbjct: 267 VKQNYGEKVLVQFEDFAN 284


>gb|AAK83073.1|AF288920_1 putative cytosolic NADP-malic enzyme [Flaveria pringlei]
          Length = 589

 Score =  158 bits (399), Expect(3) = e-114
 Identities = 74/80 (92%), Positives = 79/80 (98%)
 Frame = -3

Query: 561 NVEELLPIVYTPVVGEACQKYGSIFKRPQGLYISLKEKGRILEVLKNWPEKSIQVIVVTD 382
           NVEELLP+VYTP VGEACQKYGSIFKRPQGLYISLKEKG+ILEVL+NWPE++IQVIVVTD
Sbjct: 128 NVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKILEVLRNWPERNIQVIVVTD 187

Query: 381 GERILGLGDLGCQGMGIPVG 322
           GERILGLGDLGCQGMGIPVG
Sbjct: 188 GERILGLGDLGCQGMGIPVG 207



 Score =  155 bits (393), Expect(3) = e-114
 Identities = 77/128 (60%), Positives = 96/128 (75%), Gaps = 1/128 (0%)
 Frame = -2

Query: 1054 DSTLKDMKNGE-LESNNSGVAGGVEDMYGEDRATQDQPVTPWAISVASGHTXXXXXXXXX 878
            +S LK MK GE +   +S V GGV+D+YGEDRA++DQ +TPW +SVASG+T         
Sbjct: 2    ESILKQMKEGESVVDPSSAVGGGVDDVYGEDRASEDQLITPWTVSVASGYTL-------- 53

Query: 877  XXXXXXMRDPRHNKGTAFTEKERDAHYLRGLLPPVVLSQELQEKKMIQNIRSYEVPLQKY 698
                  +RDP HNKG AFTE+ERD+HYLRGLLPP V +QELQEKK++QNIRSYEVPL +Y
Sbjct: 54   ------LRDPHHNKGLAFTERERDSHYLRGLLPPAVATQELQEKKLMQNIRSYEVPLHRY 107

Query: 697  TAMMDLQE 674
             AMM+L+E
Sbjct: 108  VAMMELEE 115



 Score =  148 bits (373), Expect(3) = e-114
 Identities = 70/78 (89%), Positives = 76/78 (97%)
 Frame = -2

Query: 235 GKLALYTALGGVRPSVCLPITIDVGTNNQKLLDDEFYIGLKQKRATGQEYAELLEEFMTA 56
           GKLALYTALGGVRPS CLPITIDVGTNNQKLLDDEFYIGL+QKRATG+EY +LLEEFM+A
Sbjct: 207 GKLALYTALGGVRPSACLPITIDVGTNNQKLLDDEFYIGLRQKRATGKEYYDLLEEFMSA 266

Query: 55  VKQNYGEKILIQFEDFAN 2
           VKQNYGEK+L+QFEDFAN
Sbjct: 267 VKQNYGEKVLVQFEDFAN 284


>ref|XP_003547744.1| PREDICTED: NADP-dependent malic enzyme-like [Glycine max]
          Length = 591

 Score =  158 bits (400), Expect(3) = e-113
 Identities = 75/80 (93%), Positives = 80/80 (100%)
 Frame = -3

Query: 561 NVEELLPIVYTPVVGEACQKYGSIFKRPQGLYISLKEKGRILEVLKNWPEKSIQVIVVTD 382
           +VEELLPIVYTPVVGEACQKYGSIFKRPQGL+ISLKEKG++LEVLKNWPE+SIQVIVVTD
Sbjct: 130 HVEELLPIVYTPVVGEACQKYGSIFKRPQGLFISLKEKGKVLEVLKNWPERSIQVIVVTD 189

Query: 381 GERILGLGDLGCQGMGIPVG 322
           GERILGLGDLGCQGMGIPVG
Sbjct: 190 GERILGLGDLGCQGMGIPVG 209



 Score =  150 bits (380), Expect(3) = e-113
 Identities = 78/130 (60%), Positives = 96/130 (73%), Gaps = 3/130 (2%)
 Frame = -2

Query: 1054 DSTLKDMKNGELE---SNNSGVAGGVEDMYGEDRATQDQPVTPWAISVASGHTXXXXXXX 884
            +STLK +++GE     S  S V+GGVED+YGED AT+DQ VTPW  SVASG++       
Sbjct: 2    ESTLKALRDGESVLDLSPRSTVSGGVEDVYGEDHATEDQLVTPWNFSVASGYSL------ 55

Query: 883  XXXXXXXXMRDPRHNKGTAFTEKERDAHYLRGLLPPVVLSQELQEKKMIQNIRSYEVPLQ 704
                    +RDP++NKG AFTEKERDAHYLRGLLPP + SQ+LQEK++I NIR Y+VPLQ
Sbjct: 56   --------LRDPQYNKGLAFTEKERDAHYLRGLLPPTITSQQLQEKQLINNIRQYQVPLQ 107

Query: 703  KYTAMMDLQE 674
            KY AMM+LQE
Sbjct: 108  KYQAMMELQE 117



 Score =  149 bits (377), Expect(3) = e-113
 Identities = 71/78 (91%), Positives = 77/78 (98%)
 Frame = -2

Query: 235 GKLALYTALGGVRPSVCLPITIDVGTNNQKLLDDEFYIGLKQKRATGQEYAELLEEFMTA 56
           GKLALYTALGGVRPS CLPITIDVGTNN+KLL+DEFYIGL+QKRATGQEY+ELL+EFMTA
Sbjct: 209 GKLALYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELLQEFMTA 268

Query: 55  VKQNYGEKILIQFEDFAN 2
           VKQNYGEK+LIQFEDFAN
Sbjct: 269 VKQNYGEKVLIQFEDFAN 286


>ref|XP_002324450.1| predicted protein [Populus trichocarpa] gi|222865884|gb|EEF03015.1|
           predicted protein [Populus trichocarpa]
          Length = 591

 Score =  159 bits (401), Expect(3) = e-113
 Identities = 74/80 (92%), Positives = 79/80 (98%)
 Frame = -3

Query: 561 NVEELLPIVYTPVVGEACQKYGSIFKRPQGLYISLKEKGRILEVLKNWPEKSIQVIVVTD 382
           NVEELLP+VYTP VGEACQKYGSIFKRPQGLYISLKEKG++L+VLKNWP+KSIQVIVVTD
Sbjct: 130 NVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSIQVIVVTD 189

Query: 381 GERILGLGDLGCQGMGIPVG 322
           GERILGLGDLGCQGMGIPVG
Sbjct: 190 GERILGLGDLGCQGMGIPVG 209



 Score =  154 bits (390), Expect(3) = e-113
 Identities = 79/130 (60%), Positives = 96/130 (73%), Gaps = 3/130 (2%)
 Frame = -2

Query: 1054 DSTLKDMKNGELESN---NSGVAGGVEDMYGEDRATQDQPVTPWAISVASGHTXXXXXXX 884
            +STLK+M++G    +    S V GGVED+YGEDRAT+DQ VTPW ISVASG T       
Sbjct: 2    ESTLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGFTL------ 55

Query: 883  XXXXXXXXMRDPRHNKGTAFTEKERDAHYLRGLLPPVVLSQELQEKKMIQNIRSYEVPLQ 704
                    +RDP+HNKG AFTEKERDAHYLRGLLPP  +SQ+LQEKK++  IR Y++PLQ
Sbjct: 56   --------LRDPQHNKGLAFTEKERDAHYLRGLLPPATISQQLQEKKLMNTIRQYQLPLQ 107

Query: 703  KYTAMMDLQE 674
            KYTAMM+L+E
Sbjct: 108  KYTAMMELEE 117



 Score =  145 bits (365), Expect(3) = e-113
 Identities = 68/78 (87%), Positives = 75/78 (96%)
 Frame = -2

Query: 235 GKLALYTALGGVRPSVCLPITIDVGTNNQKLLDDEFYIGLKQKRATGQEYAELLEEFMTA 56
           GKL+LYTALGGVRPS CLP+TIDVGTNN++LL DEFYIGL+QKRATGQEY+ELL EFMTA
Sbjct: 209 GKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKDEFYIGLRQKRATGQEYSELLHEFMTA 268

Query: 55  VKQNYGEKILIQFEDFAN 2
           VKQNYGEK+LIQFEDFAN
Sbjct: 269 VKQNYGEKVLIQFEDFAN 286