BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000965
         (1013 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001243291.1| malate dehydrogenase, cytoplasmic-like [Glyc...   568   e-160
ref|XP_002332745.1| predicted protein [Populus trichocarpa] gi|2...   565   e-159
ref|NP_001236661.1| cytosolic malate dehydrogenase [Glycine max]...   563   e-158
gb|AFK38114.1| unknown [Lotus japonicus]                              562   e-158
emb|CAC12826.1| malate dehydrogenase [Nicotiana tabacum]              560   e-157

>ref|NP_001243291.1| malate dehydrogenase, cytoplasmic-like [Glycine max]
           gi|366985127|gb|AEX09388.1| malate dehydrogenase
           [Glycine max]
          Length = 328

 Score =  568 bits (1465), Expect = e-160
 Identities = 283/310 (91%), Positives = 291/310 (93%)
 Frame = -3

Query: 930 MAKEPARVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELVD 751
           MAK+P RVLVTGAAGQIGYALVPMIARG+MLG DQPVILHMLDIPPAAE+LNGVKMELVD
Sbjct: 1   MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 750 AAFPLLKGVVATTDVVEACTGVNYAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 571
           AAFPLLKGVVATTDVVEACTGVN AVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 120

Query: 570 AAANCKVLVVANPANTNALILKEYAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK 391
           AAANCKVLVVANPANTNALILKE+APSIPEKNI+CLTRLDHNRALGQISERLN+QVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNIQVSDVK 180

Query: 390 NAIIWGNHSSTQYPDVNHATVGGKPVRELVADDEWLNGEFIKTVQQRGAAIIKARKXXXX 211
           N IIWGNHSSTQYPDVNHATVG KPVREL+ADD WLNGEFI TVQQRGAAIIKARK    
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVGEKPVRELIADDAWLNGEFITTVQQRGAAIIKARKLSSA 240

Query: 210 XXXXXXACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIVQGLS 31
                 ACDHIRDWVLGTP+GTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEW IVQGL 
Sbjct: 241 LSAASAACDHIRDWVLGTPQGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWTIVQGLP 300

Query: 30  IDEFSRKKLD 1
           IDEFSRKKLD
Sbjct: 301 IDEFSRKKLD 310


>ref|XP_002332745.1| predicted protein [Populus trichocarpa] gi|222833073|gb|EEE71550.1|
           predicted protein [Populus trichocarpa]
          Length = 332

 Score =  565 bits (1455), Expect = e-159
 Identities = 287/314 (91%), Positives = 290/314 (92%), Gaps = 4/314 (1%)
 Frame = -3

Query: 930 MAKEPARVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELVD 751
           MAKEPARVLVTGAAGQIGYALVPMIARG+MLG DQPVILHMLDIPPAAEALNGVKMELVD
Sbjct: 1   MAKEPARVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAEALNGVKMELVD 60

Query: 750 AAFPLLKGVVATTDVVEACTGVNYAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 571
           AAFPLLKGVVATTDVVEACTGVN AVMVGGFPRKEGMERKDVM+KNVSIYKSQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120

Query: 570 AAANCKVLVVANPANTNALILKEYAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK 391
           AAANCKVLVVANPANTNALILKE+APSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 390 NAIIWGNHSSTQYPDVNHATV----GGKPVRELVADDEWLNGEFIKTVQQRGAAIIKARK 223
           N IIWGNHSSTQYPDVNHATV    G KPVRELV DD WLN EFI TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVKDDAWLNAEFITTVQQRGAAIIKARK 240

Query: 222 XXXXXXXXXXACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIV 43
                     ACDHI DWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTC NGEWKIV
Sbjct: 241 LSSALSAASSACDHIHDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCQNGEWKIV 300

Query: 42  QGLSIDEFSRKKLD 1
           QGLSIDEFSRKKLD
Sbjct: 301 QGLSIDEFSRKKLD 314


>ref|NP_001236661.1| cytosolic malate dehydrogenase [Glycine max]
           gi|42521311|gb|AAS18241.1| cytosolic malate
           dehydrogenase [Glycine max]
          Length = 332

 Score =  563 bits (1450), Expect = e-158
 Identities = 284/314 (90%), Positives = 291/314 (92%), Gaps = 4/314 (1%)
 Frame = -3

Query: 930 MAKEPARVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELVD 751
           MAK+P RVLVTGAAGQIGYALVPMIARG+MLG DQPVILHMLDIPPAAE+LNGVKMELVD
Sbjct: 1   MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 750 AAFPLLKGVVATTDVVEACTGVNYAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 571
           AAFPLLKGVVATTDVVEACTGVN AVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 120

Query: 570 AAANCKVLVVANPANTNALILKEYAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK 391
           AAANCKVLVVANPANTNALILKE+APSIPEKNI+CLTRLDHNRALGQISERL VQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLTVQVSDVK 180

Query: 390 NAIIWGNHSSTQYPDVNHATV----GGKPVRELVADDEWLNGEFIKTVQQRGAAIIKARK 223
           N IIWGNHSSTQYPDVNHATV    G KPVREL+ADD WLNGEFI TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVATSAGEKPVRELIADDAWLNGEFITTVQQRGAAIIKARK 240

Query: 222 XXXXXXXXXXACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIV 43
                     ACDHIRDWVLGTP+GTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEW IV
Sbjct: 241 LSSALSAASAACDHIRDWVLGTPQGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWAIV 300

Query: 42  QGLSIDEFSRKKLD 1
           QGLSIDEFSRKKLD
Sbjct: 301 QGLSIDEFSRKKLD 314


>gb|AFK38114.1| unknown [Lotus japonicus]
          Length = 332

 Score =  562 bits (1449), Expect = e-158
 Identities = 284/314 (90%), Positives = 292/314 (92%), Gaps = 4/314 (1%)
 Frame = -3

Query: 930 MAKEPARVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELVD 751
           MAK+P RVLVTGAAGQIGYALVPMIARG+MLG DQPVILHMLDIPPAAE+LNGVKMELVD
Sbjct: 1   MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 750 AAFPLLKGVVATTDVVEACTGVNYAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 571
           AAFPLLKGVVATTDVVEACTGVN AVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 120

Query: 570 AAANCKVLVVANPANTNALILKEYAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK 391
           AAANCKVLVVANPANTNALILKE+APSIPE+NI+CLTRLDHNRALGQISERL+V VSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLSVPVSDVK 180

Query: 390 NAIIWGNHSSTQYPDVNHATV----GGKPVRELVADDEWLNGEFIKTVQQRGAAIIKARK 223
           N IIWGNHSSTQYPDVNHATV    G KPVRELV+DD WLNGEFI TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKTPAGEKPVRELVSDDTWLNGEFITTVQQRGAAIIKARK 240

Query: 222 XXXXXXXXXXACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIV 43
                     ACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIV
Sbjct: 241 LSSALSAASGACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIV 300

Query: 42  QGLSIDEFSRKKLD 1
           QGLSIDEFSRKKLD
Sbjct: 301 QGLSIDEFSRKKLD 314


>emb|CAC12826.1| malate dehydrogenase [Nicotiana tabacum]
          Length = 332

 Score =  560 bits (1442), Expect = e-157
 Identities = 283/314 (90%), Positives = 288/314 (91%), Gaps = 4/314 (1%)
 Frame = -3

Query: 930 MAKEPARVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELVD 751
           MAK+P RVLVTGAAGQIGYALVPMIARG+MLGADQPVILHMLDIPPAAEALNGVKMELVD
Sbjct: 1   MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPAAEALNGVKMELVD 60

Query: 750 AAFPLLKGVVATTDVVEACTGVNYAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKH 571
           AAFPLLKGVVATTD VEACTGVN AVMVGGFPRKEGMERKDVM+KNVSIYKSQASALEKH
Sbjct: 61  AAFPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120

Query: 570 AAANCKVLVVANPANTNALILKEYAPSIPEKNITCLTRLDHNRALGQISERLNVQVSDVK 391
           AA NCKVLVVANPANTNALILKEYAPSIPEKNI+CLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEYAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 390 NAIIWGNHSSTQYPDVNHATV----GGKPVRELVADDEWLNGEFIKTVQQRGAAIIKARK 223
           N IIWGNHSS+QYPDVNHATV    G KPVRELVADD WLNGEFI TVQQRGAAIIKARK
Sbjct: 181 NVIIWGNHSSSQYPDVNHATVATPAGEKPVRELVADDAWLNGEFISTVQQRGAAIIKARK 240

Query: 222 XXXXXXXXXXACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCANGEWKIV 43
                     ACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPV C NGEW IV
Sbjct: 241 LSSALSAASSACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVACKNGEWSIV 300

Query: 42  QGLSIDEFSRKKLD 1
           QGL IDEFSRKKLD
Sbjct: 301 QGLPIDEFSRKKLD 314