BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000895
         (1735 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278413.1| PREDICTED: phospholipase C 3-like [Vitis vin...   832   0.0  
ref|XP_004160171.1| PREDICTED: phospholipase C 3-like [Cucumis s...   823   0.0  
ref|XP_004143151.1| PREDICTED: phospholipase C 3-like [Cucumis s...   823   0.0  
ref|XP_002299814.1| predicted protein [Populus trichocarpa] gi|2...   821   0.0  
ref|NP_172203.2| phospholipase C [Arabidopsis thaliana] gi|21703...   809   0.0  

>ref|XP_002278413.1| PREDICTED: phospholipase C 3-like [Vitis vinifera]
          Length = 532

 Score =  832 bits (2150), Expect = 0.0
 Identities = 398/529 (75%), Positives = 456/529 (86%), Gaps = 1/529 (0%)
 Frame = -3

Query: 1589 MIGRRYFLTVVFLHFLLMKTQSFPFAKKDR-KIEGPVKTIVVLVMENRSFDHIFGWIKKT 1413
            M  RR  L  V L +L+  ++S  F    + KI GP+KT+VVLVMENRSFDH+ GW+K  
Sbjct: 1    MASRRALLAAVVLLYLVFSSESLDFNIHGKYKIHGPIKTLVVLVMENRSFDHVLGWLKSA 60

Query: 1412 RPDINGLTGQEFNYLNASDPTSPKILVTDNAFYVDSDPGHSIQAIREQIFGSNIASSDPA 1233
            RP+I+GLTG+E N +NASDP SP+I V+D+A +VDSDPGHS QAIREQ+FG+     +PA
Sbjct: 61   RPEIDGLTGKESNRVNASDPDSPEIWVSDDALFVDSDPGHSFQAIREQVFGTGDWFQNPA 120

Query: 1232 PMNGFAQQAASMGVDGLPETVMSGFKPELLPIYSELANEFAVFDRWFASVPASTQPNRFF 1053
            PM+GFAQQA +M  +GL +TVMSGFKP +LP+Y+ LANEFAVFDRWFA+VPASTQPNRF+
Sbjct: 121  PMSGFAQQAKNMS-EGLSKTVMSGFKPAVLPVYTALANEFAVFDRWFAAVPASTQPNRFY 179

Query: 1052 VHSATSHGASSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFQSLRKLKHIT 873
            VHSATSHGA+SNVRKDLIHGFPQ+TIFDSLDEN L+FGIYYQNIPATLFF+SLRKL+H+T
Sbjct: 180  VHSATSHGATSNVRKDLIHGFPQRTIFDSLDENDLSFGIYYQNIPATLFFKSLRKLRHVT 239

Query: 872  KFHNYALKFKSDAKNGNLPNYVVVEQRYFDNKLFPANDDHPSHDVSLGQSLVKEVYEVLR 693
            +FH+YALKFK  AK G LPNYVV+EQRYFD K FPANDDHPSHDV++GQ  VKEVYE+LR
Sbjct: 240  RFHDYALKFKLHAKRGKLPNYVVIEQRYFDVKEFPANDDHPSHDVAIGQKFVKEVYEILR 299

Query: 692  ASPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPYYFRFDRLGVRVPTLLIS 513
            +SPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDP+YFRFDRLGVRVPT+L+S
Sbjct: 300  SSPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPFYFRFDRLGVRVPTILVS 359

Query: 512  PWIEKGLVIHQPSGPTASSEFEHSSIPATVKKLFNLDSNFLTKRDAWAGTFEKYFFLHDN 333
            PWIEKG VIH+P+GPT  S+FEHSSIPATVKKLFNL SNFLTKRDAWAGTFE YF+  D 
Sbjct: 360  PWIEKGTVIHEPTGPTPHSQFEHSSIPATVKKLFNLKSNFLTKRDAWAGTFENYFYFRDT 419

Query: 332  PRDDCPEKLPEVTKSLRSGGPNEDARLSEFQIELIQLASQLNGDYLLNTYPDIGKSLTVA 153
            PRDDCPE LPEVT  LR  GP ED  LSEFQ+ELIQLASQLNGDY+LNTYP IGKS+TV 
Sbjct: 420  PRDDCPETLPEVTTPLRPRGPKEDLSLSEFQVELIQLASQLNGDYVLNTYPYIGKSMTVG 479

Query: 152  EGSKYAADAIERFLEAGRAALRTGANDSAMVTMRPSLTSRTSGEEYSNY 6
            E ++YA DA+ RFLEAG+AALR GANDSA+VTMRPSLTSRT G +Y  Y
Sbjct: 480  EANRYAEDAVRRFLEAGKAALRAGANDSAIVTMRPSLTSRTMGPDYKKY 528


>ref|XP_004160171.1| PREDICTED: phospholipase C 3-like [Cucumis sativus]
          Length = 534

 Score =  823 bits (2126), Expect = 0.0
 Identities = 392/523 (74%), Positives = 456/523 (87%), Gaps = 2/523 (0%)
 Frame = -3

Query: 1589 MIGRRYFLTVVFLHFLLMKTQSFPFAKKDRK--IEGPVKTIVVLVMENRSFDHIFGWIKK 1416
            M+ R   LT  FL +LL  + +F F  K R+  I+GP+K++VVLVMENRSFDH+ GW+K 
Sbjct: 1    MVLREAVLTSFFLIYLLFSSHAFEFDFKKRRHEIQGPIKSVVVLVMENRSFDHVLGWLKS 60

Query: 1415 TRPDINGLTGQEFNYLNASDPTSPKILVTDNAFYVDSDPGHSIQAIREQIFGSNIASSDP 1236
             RP+I+GLTG+E N ++ SDP S ++ V+++A ++DSDPGHS QAIREQIFGSN +S +P
Sbjct: 61   VRPEIDGLTGKESNRVSVSDPNSEEVFVSNDAIFIDSDPGHSFQAIREQIFGSNDSSVNP 120

Query: 1235 APMNGFAQQAASMGVDGLPETVMSGFKPELLPIYSELANEFAVFDRWFASVPASTQPNRF 1056
            APMNGFAQQAA+M    +P+TVMSGFKPE +P+Y+ELAN+FAVFDRWFASVPASTQPNRF
Sbjct: 121  APMNGFAQQAAAMDEIDMPKTVMSGFKPERVPVYTELANQFAVFDRWFASVPASTQPNRF 180

Query: 1055 FVHSATSHGASSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFQSLRKLKHI 876
            +VHSATSHGA SNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFF+SLRKLKHI
Sbjct: 181  YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFKSLRKLKHI 240

Query: 875  TKFHNYALKFKSDAKNGNLPNYVVVEQRYFDNKLFPANDDHPSHDVSLGQSLVKEVYEVL 696
             KFH+YALKFK  AK G LPNY V+EQRYFD  L+PANDDHPSHDV+ GQ  VKEVYE+L
Sbjct: 241  VKFHSYALKFKLHAKLGRLPNYAVIEQRYFDVDLYPANDDHPSHDVARGQKFVKEVYEIL 300

Query: 695  RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPYYFRFDRLGVRVPTLLI 516
            RASPQWKEMALLITYDEHGGFYDHVPTPV+GVPNPDGIIGPDPYYFRFDRLGVRVPT+L+
Sbjct: 301  RASPQWKEMALLITYDEHGGFYDHVPTPVTGVPNPDGIIGPDPYYFRFDRLGVRVPTILV 360

Query: 515  SPWIEKGLVIHQPSGPTASSEFEHSSIPATVKKLFNLDSNFLTKRDAWAGTFEKYFFLHD 336
            SPW+EKG VIH+P GPT++S+FEHSS+PATVKKLFNL SNFLTKRDAWAGTF+ Y  L D
Sbjct: 361  SPWVEKGTVIHEPVGPTSTSQFEHSSVPATVKKLFNLKSNFLTKRDAWAGTFDHYLKLRD 420

Query: 335  NPRDDCPEKLPEVTKSLRSGGPNEDARLSEFQIELIQLASQLNGDYLLNTYPDIGKSLTV 156
            +PRDDCPE LP+VT  LR  GP E A+LSEFQ+ELIQLASQLNGD++LN+YP+IGK +TV
Sbjct: 421  HPRDDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDHVLNSYPNIGKYMTV 480

Query: 155  AEGSKYAADAIERFLEAGRAALRTGANDSAMVTMRPSLTSRTS 27
             E +KYA DA++RFLEAGR AL  GAN+SA+VTMRPSLTSRT+
Sbjct: 481  GEANKYAEDAVKRFLEAGRVALMAGANESAIVTMRPSLTSRTT 523


>ref|XP_004143151.1| PREDICTED: phospholipase C 3-like [Cucumis sativus]
          Length = 534

 Score =  823 bits (2126), Expect = 0.0
 Identities = 392/523 (74%), Positives = 456/523 (87%), Gaps = 2/523 (0%)
 Frame = -3

Query: 1589 MIGRRYFLTVVFLHFLLMKTQSFPFAKKDRK--IEGPVKTIVVLVMENRSFDHIFGWIKK 1416
            M+ R   LT  FL +LL  + +F F  K R+  I+GP+K++VVLVMENRSFDH+ GW+K 
Sbjct: 1    MVLREAVLTSFFLIYLLFSSHAFEFDFKKRRHEIQGPIKSVVVLVMENRSFDHVLGWLKS 60

Query: 1415 TRPDINGLTGQEFNYLNASDPTSPKILVTDNAFYVDSDPGHSIQAIREQIFGSNIASSDP 1236
             RP+I+GLTG+E N ++ SDP S ++ V+++A ++DSDPGHS QAIREQIFGSN +S +P
Sbjct: 61   VRPEIDGLTGKESNRVSVSDPNSEEVFVSNDAIFIDSDPGHSFQAIREQIFGSNDSSVNP 120

Query: 1235 APMNGFAQQAASMGVDGLPETVMSGFKPELLPIYSELANEFAVFDRWFASVPASTQPNRF 1056
            APMNGFAQQAA+M    +P+TVMSGFKPE +P+Y+ELAN+FAVFDRWFASVPASTQPNRF
Sbjct: 121  APMNGFAQQAAAMDEIDMPKTVMSGFKPERVPVYTELANQFAVFDRWFASVPASTQPNRF 180

Query: 1055 FVHSATSHGASSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFQSLRKLKHI 876
            +VHSATSHGA SNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFF+SLRKLKHI
Sbjct: 181  YVHSATSHGAMSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFKSLRKLKHI 240

Query: 875  TKFHNYALKFKSDAKNGNLPNYVVVEQRYFDNKLFPANDDHPSHDVSLGQSLVKEVYEVL 696
             KFH+YALKFK  AK G LPNY V+EQRYFD  L+PANDDHPSHDV+ GQ  VKEVYE+L
Sbjct: 241  VKFHSYALKFKLHAKLGRLPNYAVIEQRYFDVDLYPANDDHPSHDVARGQKFVKEVYEIL 300

Query: 695  RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPYYFRFDRLGVRVPTLLI 516
            RASPQWKEMALLITYDEHGGFYDHVPTPV+GVPNPDGIIGPDPYYFRFDRLGVRVPT+L+
Sbjct: 301  RASPQWKEMALLITYDEHGGFYDHVPTPVTGVPNPDGIIGPDPYYFRFDRLGVRVPTILV 360

Query: 515  SPWIEKGLVIHQPSGPTASSEFEHSSIPATVKKLFNLDSNFLTKRDAWAGTFEKYFFLHD 336
            SPW+EKG VIH+P GPT++S+FEHSS+PATVKKLFNL SNFLTKRDAWAGTF+ Y  L D
Sbjct: 361  SPWVEKGTVIHEPVGPTSTSQFEHSSVPATVKKLFNLKSNFLTKRDAWAGTFDHYLKLRD 420

Query: 335  NPRDDCPEKLPEVTKSLRSGGPNEDARLSEFQIELIQLASQLNGDYLLNTYPDIGKSLTV 156
            +PRDDCPE LP+VT  LR  GP E A+LSEFQ+ELIQLASQLNGD++LN+YP+IGK +TV
Sbjct: 421  HPRDDCPETLPKVTAPLRPWGPKEHAKLSEFQVELIQLASQLNGDHVLNSYPNIGKYMTV 480

Query: 155  AEGSKYAADAIERFLEAGRAALRTGANDSAMVTMRPSLTSRTS 27
             E +KYA DA++RFLEAGR AL  GAN+SA+VTMRPSLTSRT+
Sbjct: 481  GEANKYAEDAVKRFLEAGRVALMAGANESAIVTMRPSLTSRTT 523


>ref|XP_002299814.1| predicted protein [Populus trichocarpa] gi|222847072|gb|EEE84619.1|
            predicted protein [Populus trichocarpa]
          Length = 535

 Score =  821 bits (2120), Expect = 0.0
 Identities = 393/532 (73%), Positives = 454/532 (85%), Gaps = 4/532 (0%)
 Frame = -3

Query: 1589 MIGRRYFLTVVFLHFLLMKTQSFPF----AKKDRKIEGPVKTIVVLVMENRSFDHIFGWI 1422
            M  RR  LT +   +L++ +QS  F     +   KI+GP+KT+VVLVMENRSFDH+ GW+
Sbjct: 1    MTSRRLSLTSLLFLYLVVSSQSLDFDDLHKRHKPKIKGPIKTLVVLVMENRSFDHVLGWL 60

Query: 1421 KKTRPDINGLTGQEFNYLNASDPTSPKILVTDNAFYVDSDPGHSIQAIREQIFGSNIASS 1242
            K TRPDI+GL+G E N ++ASD  S ++ V+D+A ++DSDPGHS QAIREQIFGSN + +
Sbjct: 61   KSTRPDIDGLSGSESNRISASDRNSDEVFVSDDAVFIDSDPGHSFQAIREQIFGSNDSFA 120

Query: 1241 DPAPMNGFAQQAASMGVDGLPETVMSGFKPELLPIYSELANEFAVFDRWFASVPASTQPN 1062
            DPAPMNGF QQA SMG + + +TVMSGFKP LLP+Y+ELANEFAVFDRWFASVPASTQPN
Sbjct: 121  DPAPMNGFVQQAKSMG-ETMSKTVMSGFKPSLLPVYTELANEFAVFDRWFASVPASTQPN 179

Query: 1061 RFFVHSATSHGASSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFQSLRKLK 882
            RF+VHSATSHGA SNVRKDLIHGFPQ+TIFDSLD+NGL+FGIYYQNIPATLFF+SLRKLK
Sbjct: 180  RFYVHSATSHGAMSNVRKDLIHGFPQRTIFDSLDDNGLSFGIYYQNIPATLFFKSLRKLK 239

Query: 881  HITKFHNYALKFKSDAKNGNLPNYVVVEQRYFDNKLFPANDDHPSHDVSLGQSLVKEVYE 702
            H+ KFH+Y LKFK  AK G LPNYVVVEQRYFD +LFPANDDHPSHDV+ GQ  VKEVYE
Sbjct: 240  HLLKFHSYELKFKLHAKLGKLPNYVVVEQRYFDVELFPANDDHPSHDVARGQRFVKEVYE 299

Query: 701  VLRASPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPYYFRFDRLGVRVPTL 522
            +LR+SPQWKEMALLITYDEHGGFYDHVPTPV GVPNPDGIIGPDPYYF+FDRLG+RVPT 
Sbjct: 300  ILRSSPQWKEMALLITYDEHGGFYDHVPTPVRGVPNPDGIIGPDPYYFQFDRLGIRVPTF 359

Query: 521  LISPWIEKGLVIHQPSGPTASSEFEHSSIPATVKKLFNLDSNFLTKRDAWAGTFEKYFFL 342
            LISPWI+KG VIH+P GP   S+FEHSSIPATVKKLFNL+SNFLT+RDAWAG+FE YF+L
Sbjct: 360  LISPWIDKGTVIHEPDGPRPDSQFEHSSIPATVKKLFNLNSNFLTRRDAWAGSFENYFYL 419

Query: 341  HDNPRDDCPEKLPEVTKSLRSGGPNEDARLSEFQIELIQLASQLNGDYLLNTYPDIGKSL 162
             D PRDDCPE LP+VT SLR  GP EDA LSEFQ+E+IQLASQLNGD++LN YPDIGKS+
Sbjct: 420  RDTPRDDCPETLPDVTTSLRPWGPKEDASLSEFQVEMIQLASQLNGDHVLNAYPDIGKSM 479

Query: 161  TVAEGSKYAADAIERFLEAGRAALRTGANDSAMVTMRPSLTSRTSGEEYSNY 6
            TV E ++YA DA+ RFLEAGRAALR GAN+SA+VTM+PSLTSR    +   Y
Sbjct: 480  TVGEANRYAEDAVRRFLEAGRAALRAGANESAIVTMKPSLTSRVPAGDNGRY 531


>ref|NP_172203.2| phospholipase C [Arabidopsis thaliana] gi|21703147|gb|AAM74513.1|
            At1g07230/F10K1_4 [Arabidopsis thaliana]
            gi|24417127|dbj|BAC22506.1| phosphatidylglycerol specific
            phospholipase C [Arabidopsis thaliana]
            gi|58652062|gb|AAW80856.1| At1g07230 [Arabidopsis
            thaliana] gi|332189974|gb|AEE28095.1| phospholipase C
            [Arabidopsis thaliana]
          Length = 533

 Score =  809 bits (2089), Expect = 0.0
 Identities = 394/531 (74%), Positives = 451/531 (84%), Gaps = 2/531 (0%)
 Frame = -3

Query: 1589 MIGRRYFLTVVFLHFLLMKTQSFPF--AKKDRKIEGPVKTIVVLVMENRSFDHIFGWIKK 1416
            M  RR   TV+   +LL+ +QS  F  ++K  KI+GP+KTIVV+VMENRSFDHI GW+K 
Sbjct: 1    MAFRRVLTTVILFCYLLISSQSIEFKNSQKPHKIQGPIKTIVVVVMENRSFDHILGWLKS 60

Query: 1415 TRPDINGLTGQEFNYLNASDPTSPKILVTDNAFYVDSDPGHSIQAIREQIFGSNIASSDP 1236
            TRP+I+GLTG+E N LN SDP S KI V+D+A +VD DPGHS QAIREQIFGSN  S DP
Sbjct: 61   TRPEIDGLTGKESNPLNVSDPNSKKIFVSDDAVFVDMDPGHSFQAIREQIFGSNDTSGDP 120

Query: 1235 APMNGFAQQAASMGVDGLPETVMSGFKPELLPIYSELANEFAVFDRWFASVPASTQPNRF 1056
              MNGFAQQ+ SM   G+ + VMSGFKPE+LP+Y+ELANEF VFDRWFASVP STQPNRF
Sbjct: 121  K-MNGFAQQSESME-PGMAKNVMSGFKPEVLPVYTELANEFGVFDRWFASVPTSTQPNRF 178

Query: 1055 FVHSATSHGASSNVRKDLIHGFPQKTIFDSLDENGLTFGIYYQNIPATLFFQSLRKLKHI 876
            +VHSATSHG SSNV+KDL+ GFPQKTIFDSLDENGL+FGIYYQNIPAT FF+SLR+LKH+
Sbjct: 179  YVHSATSHGCSSNVKKDLVKGFPQKTIFDSLDENGLSFGIYYQNIPATFFFKSLRRLKHL 238

Query: 875  TKFHNYALKFKSDAKNGNLPNYVVVEQRYFDNKLFPANDDHPSHDVSLGQSLVKEVYEVL 696
             KFH+YALKFK DAK G LPNY VVEQRYFD  LFPANDDHPSHDV+ GQ  VKEVYE L
Sbjct: 239  VKFHSYALKFKLDAKLGKLPNYSVVEQRYFDIDLFPANDDHPSHDVAAGQRFVKEVYETL 298

Query: 695  RASPQWKEMALLITYDEHGGFYDHVPTPVSGVPNPDGIIGPDPYYFRFDRLGVRVPTLLI 516
            R+SPQWKEMALLITYDEHGGFYDHVPTPV GVPNPDGIIGPDP+YF FDRLGVRVPT LI
Sbjct: 299  RSSPQWKEMALLITYDEHGGFYDHVPTPVKGVPNPDGIIGPDPFYFGFDRLGVRVPTFLI 358

Query: 515  SPWIEKGLVIHQPSGPTASSEFEHSSIPATVKKLFNLDSNFLTKRDAWAGTFEKYFFLHD 336
            SPWIEKG VIH+P GPT  S+FEHSSIPATVKKLFNL S+FLTKRDAWAGTFEKYF + D
Sbjct: 359  SPWIEKGTVIHEPEGPTPHSQFEHSSIPATVKKLFNLKSHFLTKRDAWAGTFEKYFRIRD 418

Query: 335  NPRDDCPEKLPEVTKSLRSGGPNEDARLSEFQIELIQLASQLNGDYLLNTYPDIGKSLTV 156
            +PR DCPEKLPEV  SLR  G  ED++LSEFQ+ELIQLASQL GD+LLN+YPDIGK++TV
Sbjct: 419  SPRQDCPEKLPEVKLSLRPWGAKEDSKLSEFQVELIQLASQLVGDHLLNSYPDIGKNMTV 478

Query: 155  AEGSKYAADAIERFLEAGRAALRTGANDSAMVTMRPSLTSRTSGEEYSNYF 3
            +EG+KYA DA+++FLEAG AAL  GA+++ +VTMRPSLT+RTS  E +N +
Sbjct: 479  SEGNKYAEDAVQKFLEAGMAALEAGADENTIVTMRPSLTTRTSPSEGTNKY 529