BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000821
         (1218 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P37706.2|RRFC_DAUCA RecName: Full=Ribosome-recycling factor, ...   347   5e-93
gb|ABK96574.1| unknown [Populus trichocarpa x Populus deltoides]      319   1e-84
ref|XP_002302112.1| predicted protein [Populus trichocarpa] gi|2...   318   2e-84
emb|CBI28691.3| unnamed protein product [Vitis vinifera]              311   2e-82
ref|XP_004140097.1| PREDICTED: ribosome-recycling factor, chloro...   311   3e-82

>sp|P37706.2|RRFC_DAUCA RecName: Full=Ribosome-recycling factor, chloroplastic; Short=RRF;
           AltName: Full=Nuclear located protein D2; AltName:
           Full=Ribosome-releasing factor, chloroplastic; Flags:
           Precursor
          Length = 227

 Score =  347 bits (889), Expect = 5e-93
 Identities = 184/230 (80%), Positives = 200/230 (86%)
 Frame = -1

Query: 942 WTAAANYAKLQVGNAKSCHKTMVLSQLAQKRRGTVRCATMEEIEAEKSLIEKDVRERMEK 763
           WTAAANY K++VG  K   KT+VLSQ   KR GT++CATMEEIEAEKSLIEK V+ERMEK
Sbjct: 1   WTAAANYVKIKVGTGKFARKTVVLSQ---KRTGTLKCATMEEIEAEKSLIEKSVKERMEK 57

Query: 762 TIENVRASFSSIRTGRSNPAMLDKIEVEYYGTPTSLKSIAQISTPDPSSLLINPYDKSSL 583
           TIENV+ASF+SIRT RSNP MLDKI+VEYYGTPTSLKSIAQISTPD SSLL+NPYDKSSL
Sbjct: 58  TIENVKASFNSIRTXRSNPDMLDKIKVEYYGTPTSLKSIAQISTPDSSSLLVNPYDKSSL 117

Query: 582 KAIEKAIVNSDLGMTPNNDGEVIRLSIPQLTADRRKELTRVVAKQAEEGKVALRNIRRDA 403
           K IEKAIVNSDLG+TPNNDG+VIRLSIPQLTADRRKEL+++VAKQAEEGKVALRNIRRDA
Sbjct: 118 KDIEKAIVNSDLGITPNNDGDVIRLSIPQLTADRRKELSKIVAKQAEEGKVALRNIRRDA 177

Query: 402 IXXXXXXXXXXXXXXDNVKDLASDLQKVIDEYIKKVDSILKQKEKELMTV 253
           I              DNVKDL+SDLQKVIDEYIKKVDSI KQKEKELMTV
Sbjct: 178 IKSYDKLEKEKKLSEDNVKDLSSDLQKVIDEYIKKVDSIFKQKEKELMTV 227


>gb|ABK96574.1| unknown [Populus trichocarpa x Populus deltoides]
          Length = 286

 Score =  319 bits (817), Expect = 1e-84
 Identities = 169/265 (63%), Positives = 208/265 (78%), Gaps = 6/265 (2%)
 Frame = -1

Query: 1029 PNPPKIALSLKS---KGPNCVKF---NLTYGCVKSWTAAANYAKLQVGNAKSCHKTMVLS 868
            PNPPK  LSL     +GP+  K    ++    V  W+++ANY+ L+ G++K   K + + 
Sbjct: 22   PNPPKALLSLTGSFHRGPHRAKVGYNSIGAASVSLWSSSANYSSLRNGSSKLSGKPVAVK 81

Query: 867  QLAQKRRGTVRCATMEEIEAEKSLIEKDVRERMEKTIENVRASFSSIRTGRSNPAMLDKI 688
            +L Q RRG VR AT+EEIEAEKSLIE D + RMEKTI+ +R +F+S+RTGR+NP+MLDKI
Sbjct: 82   RLLQNRRGVVRNATIEEIEAEKSLIEDDAKGRMEKTIDTMRTNFNSVRTGRANPSMLDKI 141

Query: 687  EVEYYGTPTSLKSIAQISTPDPSSLLINPYDKSSLKAIEKAIVNSDLGMTPNNDGEVIRL 508
            EVEYYGTP SLKSIAQISTPD SSLL+ PYDKSSLKAIEKAIV+SDLG+TPNNDGEVIR+
Sbjct: 142  EVEYYGTPVSLKSIAQISTPDASSLLVQPYDKSSLKAIEKAIVSSDLGLTPNNDGEVIRM 201

Query: 507  SIPQLTADRRKELTRVVAKQAEEGKVALRNIRRDAIXXXXXXXXXXXXXXDNVKDLASDL 328
            SIPQLT++RRKEL+++VAK AEEGKVALRNIRRDA+              DNVKDL+SDL
Sbjct: 202  SIPQLTSERRKELSKMVAKLAEEGKVALRNIRRDALKAYEKLEKEKKLSEDNVKDLSSDL 261

Query: 327  QKVIDEYIKKVDSILKQKEKELMTV 253
            QK+ DEY+KK+D+I KQKEKEL+ V
Sbjct: 262  QKLTDEYMKKLDNIFKQKEKELLKV 286


>ref|XP_002302112.1| predicted protein [Populus trichocarpa] gi|222843838|gb|EEE81385.1|
            predicted protein [Populus trichocarpa]
          Length = 286

 Score =  318 bits (814), Expect = 2e-84
 Identities = 169/265 (63%), Positives = 208/265 (78%), Gaps = 6/265 (2%)
 Frame = -1

Query: 1029 PNPPKIALSLKS---KGPNCVKF---NLTYGCVKSWTAAANYAKLQVGNAKSCHKTMVLS 868
            PNPPK  LSL     +GP+  K    ++    V  W+++ANY+ L+ G+ K   K + + 
Sbjct: 22   PNPPKALLSLTGSFHRGPHRAKVCYNSIGATSVSLWSSSANYSSLRNGSTKLSGKPVAVK 81

Query: 867  QLAQKRRGTVRCATMEEIEAEKSLIEKDVRERMEKTIENVRASFSSIRTGRSNPAMLDKI 688
            +L Q RRG VR AT+EEIEAEKSLIE D + RMEKTI+ +RA+F+S+RTGR+NP+MLDKI
Sbjct: 82   RLLQNRRGVVRNATIEEIEAEKSLIEDDAKGRMEKTIDTMRANFNSVRTGRANPSMLDKI 141

Query: 687  EVEYYGTPTSLKSIAQISTPDPSSLLINPYDKSSLKAIEKAIVNSDLGMTPNNDGEVIRL 508
            EVEYYGTP SLKSIAQISTPD SSLL+ PYDKSSLKAIEKAIV+SDLG+TPNNDGEVIR+
Sbjct: 142  EVEYYGTPVSLKSIAQISTPDASSLLVQPYDKSSLKAIEKAIVSSDLGLTPNNDGEVIRM 201

Query: 507  SIPQLTADRRKELTRVVAKQAEEGKVALRNIRRDAIXXXXXXXXXXXXXXDNVKDLASDL 328
            SIPQLT++RRKEL+++VAK AEEGKVALRNIRRDA+              DNVKDL+SDL
Sbjct: 202  SIPQLTSERRKELSKMVAKLAEEGKVALRNIRRDALKAYEKLEKEKKLSEDNVKDLSSDL 261

Query: 327  QKVIDEYIKKVDSILKQKEKELMTV 253
            QK+ DEY+KK+++I KQKEKEL+ V
Sbjct: 262  QKLTDEYMKKLENIFKQKEKELLKV 286


>emb|CBI28691.3| unnamed protein product [Vitis vinifera]
          Length = 274

 Score =  311 bits (797), Expect = 2e-82
 Identities = 163/247 (65%), Positives = 195/247 (78%)
 Frame = -1

Query: 993 KGPNCVKFNLTYGCVKSWTAAANYAKLQVGNAKSCHKTMVLSQLAQKRRGTVRCATMEEI 814
           +GP   K +L  G   +  +AANY +L  G  +   K  V  +  QKR G  RCAT+EEI
Sbjct: 28  RGPGYEKCHLNIGIAFTLRSAANYVRLGDGAREYSGKLFVAKRNFQKRTGIFRCATIEEI 87

Query: 813 EAEKSLIEKDVRERMEKTIENVRASFSSIRTGRSNPAMLDKIEVEYYGTPTSLKSIAQIS 634
           EAEKS IEKDV+ERMEKTIE VR +F+SIRTGR+NPA+LD++EVEYYGTP SLKSIAQIS
Sbjct: 88  EAEKSSIEKDVKERMEKTIETVRTNFNSIRTGRANPAILDRVEVEYYGTPVSLKSIAQIS 147

Query: 633 TPDPSSLLINPYDKSSLKAIEKAIVNSDLGMTPNNDGEVIRLSIPQLTADRRKELTRVVA 454
           TPD +SLL+ PYDKSSLK IEKAIV+SDLG+TPNNDGEVIRLS+PQLT++RRKEL++VVA
Sbjct: 148 TPDSASLLVQPYDKSSLKVIEKAIVSSDLGLTPNNDGEVIRLSLPQLTSERRKELSKVVA 207

Query: 453 KQAEEGKVALRNIRRDAIXXXXXXXXXXXXXXDNVKDLASDLQKVIDEYIKKVDSILKQK 274
           KQ EEGKVALRNIRRDA+              DNVKDL+SDLQK+IDEY+KK+++I KQK
Sbjct: 208 KQTEEGKVALRNIRRDALKAYEKLEKDKKLSEDNVKDLSSDLQKLIDEYMKKIETIYKQK 267

Query: 273 EKELMTV 253
           EKEL+ V
Sbjct: 268 EKELLKV 274


>ref|XP_004140097.1| PREDICTED: ribosome-recycling factor, chloroplastic-like [Cucumis
            sativus]
          Length = 278

 Score =  311 bits (796), Expect = 3e-82
 Identities = 167/259 (64%), Positives = 201/259 (77%), Gaps = 2/259 (0%)
 Frame = -1

Query: 1023 PPKIALSLK--SKGPNCVKFNLTYGCVKSWTAAANYAKLQVGNAKSCHKTMVLSQLAQKR 850
            PPK  LSL+  + G NCVK       + SW +A+NY +L         +  V   + + R
Sbjct: 20   PPKSLLSLRDYNGGLNCVKCGSITAHLCSWKSASNYVRLNRQALVISTQLSVSGNVFKNR 79

Query: 849  RGTVRCATMEEIEAEKSLIEKDVRERMEKTIENVRASFSSIRTGRSNPAMLDKIEVEYYG 670
            R  VR AT+EEIEAEK+LIEKDV+ RMEKTI+ VR++F+SIRTGRSNPAMLDKIEVEYYG
Sbjct: 80   RLVVRAATIEEIEAEKTLIEKDVKARMEKTIDTVRSNFNSIRTGRSNPAMLDKIEVEYYG 139

Query: 669  TPTSLKSIAQISTPDPSSLLINPYDKSSLKAIEKAIVNSDLGMTPNNDGEVIRLSIPQLT 490
            +P SLKSIAQISTPD SSLL+ PYDKSSLK+IEKAIVNSDLGMTPNNDGEVIRL++PQLT
Sbjct: 140  SPVSLKSIAQISTPDASSLLVQPYDKSSLKSIEKAIVNSDLGMTPNNDGEVIRLTLPQLT 199

Query: 489  ADRRKELTRVVAKQAEEGKVALRNIRRDAIXXXXXXXXXXXXXXDNVKDLASDLQKVIDE 310
            ++RRKEL++VVAKQAEEGKVA+RNIRRDA+              DNVKDL+SDLQK+ D+
Sbjct: 200  SERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLEKEKKLSEDNVKDLSSDLQKLTDD 259

Query: 309  YIKKVDSILKQKEKELMTV 253
            Y+KK+D + KQKEKEL+ V
Sbjct: 260  YMKKIDVLYKQKEKELLKV 278