BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000739
         (1579 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002312450.1| DELLA domain GRAS family transcription facto...   623   0.0  
ref|XP_002284648.1| PREDICTED: DELLA protein GAI1 isoform 1 [Vit...   645   0.0  
gb|AEK06229.1| GAI1 [Vitis vinifera]                                  643   0.0  
ref|XP_002314799.1| DELLA domain GRAS family transcription facto...   619   0.0  
gb|ABG26370.1| DELLA protein GAI [Gossypium barbadense]               628   0.0  

>ref|XP_002312450.1| DELLA domain GRAS family transcription factor, GA insensitive  (GAI),
            GA1-3 1 (RGA1) repressor protein [Populus trichocarpa]
            gi|222852270|gb|EEE89817.1| DELLA domain GRAS family
            transcription factor, GA insensitive (GAI), GA1-3 1
            (RGA1) repressor protein [Populus trichocarpa]
          Length = 600

 Score =  623 bits (1606), Expect(2) = 0.0
 Identities = 313/420 (74%), Positives = 351/420 (83%), Gaps = 8/420 (1%)
 Frame = -3

Query: 1238 E*KRLKTVVSPAVESPA--------SWQVPAAESQPVPVLVDSQENGVRLVHTLMACAQA 1083
            E KRLKT +     S +        S+ +    ++PV VLVDSQENG+RLVH LMACA+A
Sbjct: 184  ESKRLKTDLYQTSSSSSLSSATTLGSFGISTESTRPV-VLVDSQENGIRLVHLLMACAEA 242

Query: 1082 VQENNLKLAEALVKQIAFLAVSQIGAMKKVATYFAEALARRIYKLYPQSPQDTSFNDMLE 903
            VQE+N  LAEALVKQI FLAVSQ G M+KVATYFAEALARRIYKL PQ+  D S +D+L+
Sbjct: 243  VQESNFTLAEALVKQIGFLAVSQAGVMRKVATYFAEALARRIYKLCPQNSTDHSLSDILQ 302

Query: 902  MHFYETCPYLKFAHFTANQAILEAFADKKMVHVIDFSMKEGMQWPALMQALALRPSGPPT 723
            +HFYETCPYLKFAHFTANQAILEAF  KK VHVIDFSM +GMQWPALMQALALRP GPP 
Sbjct: 303  IHFYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGMQWPALMQALALRPGGPPA 362

Query: 722  FRLTGIGPPSHDNTDHLQEVGWKLAQFAETIHVKFEYRGFVANTLSDLDASMLDLREGET 543
            FRLTGIGPP+HDNTDHLQEVGWKLAQ AETIHV+FEYRGFVAN+L+DLDASML+LR  E+
Sbjct: 363  FRLTGIGPPAHDNTDHLQEVGWKLAQLAETIHVEFEYRGFVANSLADLDASMLELRPTES 422

Query: 542  VAVNSVFDLHQLLARPGAIEKVMSAVADMKPEIVTIVEQEANHNGPVFLDRFTESLHYYS 363
            VAVNSVF+LH+LL+RPGAIEKV+S V  MKPEIVT+VEQEANHNGP+FLDRFTESLHYYS
Sbjct: 423  VAVNSVFELHKLLSRPGAIEKVLSVVKQMKPEIVTVVEQEANHNGPIFLDRFTESLHYYS 482

Query: 362  TLFDSLESCGNGSGGIDAGANVSNQDKVMSEVYLGRQICNIVACEGADRIERHEPLTQWT 183
            TLFDSLE             +VS QDK+MSEVYLG+QICN+VACEG DR+ERHE LTQW 
Sbjct: 483  TLFDSLE------------GSVSTQDKIMSEVYLGKQICNVVACEGPDRVERHETLTQWR 530

Query: 182  ERFHSAGFEPVHIGSNAYKQASMLLALFAGGNGYRVEEHEGCLMLGWHTRPLITTSAWKL 3
             R  S GF PVH+GSNA+KQASMLLALFAGG+GYRVEE+ GCLMLGWHTRPLI TSAW+L
Sbjct: 531  TRLGSVGFAPVHLGSNAFKQASMLLALFAGGDGYRVEENNGCLMLGWHTRPLIATSAWRL 590



 Score =  136 bits (343), Expect(2) = 0.0
 Identities = 77/122 (63%), Positives = 88/122 (72%), Gaps = 20/122 (16%)
 Frame = -1

Query: 1558 DELLAVLGYKVRSSDMAGVAEKLQHLEEAMGQAQQQGISHLASDTVHYNPSDLSSWLESM 1379
            DELLAVLGYKVRSSDMA VA+KL+ LEE MG AQ+ G+SHLASD+VHYNPSDLS+WLESM
Sbjct: 54   DELLAVLGYKVRSSDMAEVAQKLEQLEEVMGHAQEDGLSHLASDSVHYNPSDLSTWLESM 113

Query: 1378 IVELNP----QPSDDLF---AESST-------------NRIIFDDSCSNDYDLKAIPGKA 1259
            I ELNP     PS D     AESST              + +F++S S+DYDLK IPGKA
Sbjct: 114  ISELNPNLNFDPSADSLLAPAESSTITSIDFSDHKHHQQQKLFEESSSSDYDLKVIPGKA 173

Query: 1258 VF 1253
            VF
Sbjct: 174  VF 175


>ref|XP_002284648.1| PREDICTED: DELLA protein GAI1 isoform 1 [Vitis vinifera]
            gi|75159681|sp|Q8S4W7.1|GAI1_VITVI RecName: Full=DELLA
            protein GAI1; AltName: Full=Gibberellic acid-insensitive
            mutant protein 1; AltName: Full=VvGAI1
            gi|20334379|gb|AAM19210.1|AF378125_1 GAI-like protein 1
            [Vitis vinifera]
          Length = 590

 Score =  645 bits (1664), Expect(2) = 0.0
 Identities = 320/414 (77%), Positives = 358/414 (86%), Gaps = 4/414 (0%)
 Frame = -3

Query: 1232 KRLKTVVSPAVESPAS----WQVPAAESQPVPVLVDSQENGVRLVHTLMACAQAVQENNL 1065
            KRLK   S    S +S    W VP   ++PV VLVDSQE G+RLVHTLMACA+AVQ+ NL
Sbjct: 172  KRLKPTTSATANSVSSVIGGWGVPTESARPV-VLVDSQETGIRLVHTLMACAEAVQQENL 230

Query: 1064 KLAEALVKQIAFLAVSQIGAMKKVATYFAEALARRIYKLYPQSPQDTSFNDMLEMHFYET 885
            KLAEALVKQI FLAVSQ GAM+KVATYFAE LARRIY+LYP  P D+SF+D+L+MHFYET
Sbjct: 231  KLAEALVKQIGFLAVSQAGAMRKVATYFAEGLARRIYRLYPDKPLDSSFSDILQMHFYET 290

Query: 884  CPYLKFAHFTANQAILEAFADKKMVHVIDFSMKEGMQWPALMQALALRPSGPPTFRLTGI 705
            CPYLKFAHFTANQAILEAF  KK VHVIDFSMK+GMQWPALMQALALRP GPP+FRLTGI
Sbjct: 291  CPYLKFAHFTANQAILEAFEGKKRVHVIDFSMKQGMQWPALMQALALRPGGPPSFRLTGI 350

Query: 704  GPPSHDNTDHLQEVGWKLAQFAETIHVKFEYRGFVANTLSDLDASMLDLREGETVAVNSV 525
            GPPS DNTDHL EVGWKLAQ AETIHV+FEYRGFVAN+L+DLDASML+LR+GE+VAVNSV
Sbjct: 351  GPPSTDNTDHLHEVGWKLAQLAETIHVEFEYRGFVANSLADLDASMLELRDGESVAVNSV 410

Query: 524  FDLHQLLARPGAIEKVMSAVADMKPEIVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSL 345
            F+LH LLARPG IE+V+SAV DMKP+IVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSL
Sbjct: 411  FELHSLLARPGGIERVLSAVKDMKPDIVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSL 470

Query: 344  ESCGNGSGGIDAGANVSNQDKVMSEVYLGRQICNIVACEGADRIERHEPLTQWTERFHSA 165
            E CG         + V+ QDK+MSEVYLG+QICN+VACEG +R+ERHE L QW  R  SA
Sbjct: 471  EGCG--------VSPVNTQDKLMSEVYLGQQICNVVACEGPERVERHETLAQWRARLGSA 522

Query: 164  GFEPVHIGSNAYKQASMLLALFAGGNGYRVEEHEGCLMLGWHTRPLITTSAWKL 3
            GF+PV++GSNA+KQASMLLALFAGG+GYRVEE+ GCLMLGWHTRPLI TSAW+L
Sbjct: 523  GFDPVNLGSNAFKQASMLLALFAGGDGYRVEENNGCLMLGWHTRPLIATSAWQL 576



 Score =  114 bits (285), Expect(2) = 0.0
 Identities = 65/118 (55%), Positives = 80/118 (67%), Gaps = 16/118 (13%)
 Frame = -1

Query: 1558 DELLAVLGYKVRSSDMAGVAEKLQHLEEAMGQAQQQGISHLASDTVHYNPSDLSSWLESM 1379
            DELLAVLGY V++SDMA VA+KL+ LEE +  AQ+ G+SHLAS+TVHYNPSDLS+WL SM
Sbjct: 35   DELLAVLGYNVKASDMAEVAQKLEQLEEVIVNAQEDGLSHLASETVHYNPSDLSNWLGSM 94

Query: 1378 IVELNPQPS---DDLF-------------AESSTNRIIFDDSCSNDYDLKAIPGKAVF 1253
            + E NP P+   D+ F              +      IF DS S DYDLKAIPGKA++
Sbjct: 95   LSEFNPTPNCALDNPFLPPISPLDYTNCSTQPKQEPSIF-DSPSLDYDLKAIPGKALY 151


>gb|AEK06229.1| GAI1 [Vitis vinifera]
          Length = 590

 Score =  643 bits (1658), Expect(2) = 0.0
 Identities = 319/414 (77%), Positives = 357/414 (86%), Gaps = 4/414 (0%)
 Frame = -3

Query: 1232 KRLKTVVSPAVESPAS----WQVPAAESQPVPVLVDSQENGVRLVHTLMACAQAVQENNL 1065
            KR K   S    S +S    W VP   ++PV VLVDSQE G+RLVHTLMACA+AVQ+ NL
Sbjct: 172  KRSKPTASATANSVSSVIGGWGVPTESARPV-VLVDSQETGIRLVHTLMACAEAVQQENL 230

Query: 1064 KLAEALVKQIAFLAVSQIGAMKKVATYFAEALARRIYKLYPQSPQDTSFNDMLEMHFYET 885
            KLAEALVKQI FLAVSQ GAM+KVATYFAE LARRIY+LYP  P D+SF+D+L+MHFYET
Sbjct: 231  KLAEALVKQIGFLAVSQAGAMRKVATYFAEGLARRIYRLYPDKPLDSSFSDILQMHFYET 290

Query: 884  CPYLKFAHFTANQAILEAFADKKMVHVIDFSMKEGMQWPALMQALALRPSGPPTFRLTGI 705
            CPYLKFAHFTANQAILEAF  KK VHVIDFSMK+GMQWPALMQALALRP GPP+FRLTGI
Sbjct: 291  CPYLKFAHFTANQAILEAFEGKKRVHVIDFSMKQGMQWPALMQALALRPGGPPSFRLTGI 350

Query: 704  GPPSHDNTDHLQEVGWKLAQFAETIHVKFEYRGFVANTLSDLDASMLDLREGETVAVNSV 525
            GPPS DNTDHL EVGWKLAQ AETIHV+FEYRGFVAN+L+DLDASML+LR+GE+VAVNSV
Sbjct: 351  GPPSTDNTDHLHEVGWKLAQLAETIHVEFEYRGFVANSLADLDASMLELRDGESVAVNSV 410

Query: 524  FDLHQLLARPGAIEKVMSAVADMKPEIVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSL 345
            F+LH LLARPG IE+V+SAV DMKP+IVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSL
Sbjct: 411  FELHSLLARPGGIERVLSAVKDMKPDIVTIVEQEANHNGPVFLDRFTESLHYYSTLFDSL 470

Query: 344  ESCGNGSGGIDAGANVSNQDKVMSEVYLGRQICNIVACEGADRIERHEPLTQWTERFHSA 165
            E CG         + V+ QDK+MSEVYLG+QICN+VACEG +R+ERHE L QW  R  SA
Sbjct: 471  EGCG--------VSPVNTQDKLMSEVYLGQQICNVVACEGPERVERHETLAQWRARLGSA 522

Query: 164  GFEPVHIGSNAYKQASMLLALFAGGNGYRVEEHEGCLMLGWHTRPLITTSAWKL 3
            GF+PV++GSNA+KQASMLLALFAGG+GYRVEE+ GCLMLGWHTRPLI TSAW+L
Sbjct: 523  GFDPVNLGSNAFKQASMLLALFAGGDGYRVEENNGCLMLGWHTRPLIATSAWQL 576



 Score =  112 bits (281), Expect(2) = 0.0
 Identities = 64/118 (54%), Positives = 80/118 (67%), Gaps = 16/118 (13%)
 Frame = -1

Query: 1558 DELLAVLGYKVRSSDMAGVAEKLQHLEEAMGQAQQQGISHLASDTVHYNPSDLSSWLESM 1379
            DELLAVLGY V++SDMA VA++L+ LEE +  AQ+ G+SHLAS+TVHYNPSDLS+WL SM
Sbjct: 35   DELLAVLGYNVKASDMAEVAQELEQLEEVIVNAQEDGLSHLASETVHYNPSDLSNWLGSM 94

Query: 1378 IVELNPQPS---DDLF-------------AESSTNRIIFDDSCSNDYDLKAIPGKAVF 1253
            + E NP P+   D+ F              +      IF DS S DYDLKAIPGKA++
Sbjct: 95   LSEFNPTPNCALDNPFLPPISPLDYTNCSTQPKQEPSIF-DSPSLDYDLKAIPGKALY 151


>ref|XP_002314799.1| DELLA domain GRAS family transcription factor GAI/RGA1 [Populus
            trichocarpa] gi|222863839|gb|EEF00970.1| DELLA domain
            GRAS family transcription factor GAI/RGA1 [Populus
            trichocarpa]
          Length = 602

 Score =  619 bits (1595), Expect(2) = 0.0
 Identities = 315/420 (75%), Positives = 349/420 (83%), Gaps = 8/420 (1%)
 Frame = -3

Query: 1238 E*KRLKTVVSPAVESPASWQ------VPAAESQPVPVLVDSQENGVRLVHTLMACAQAVQ 1077
            E KRLKT +     SP+S        V + ES    VLVDSQENGVRLVH LMACA+AVQ
Sbjct: 185  ESKRLKTDLYQTSSSPSSSSTTLGSLVASTESTRPVVLVDSQENGVRLVHLLMACAEAVQ 244

Query: 1076 ENNLKLAEALVKQIAFLAVSQIGAMKKVATYFAEALARRIYKLYPQSPQDTSFNDMLEMH 897
            ENNL LAEALVKQI FLAVSQ GAM+KVATYFAEALARRIYKLYPQ+  D S +D+L++H
Sbjct: 245  ENNLNLAEALVKQIGFLAVSQAGAMRKVATYFAEALARRIYKLYPQNSTDHSLSDILQIH 304

Query: 896  FYETCPYLKFAHFTANQAILEAFADKKMVHVIDFSMKEGMQWPALMQALALRPSGPPTFR 717
            FYETCPYLKFAHFTANQAILEAF  KK VHVIDFSM +GMQWPALMQALALRP GPP  R
Sbjct: 305  FYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGMQWPALMQALALRPGGPPALR 364

Query: 716  LTGIGPPSHDNTDHLQEVGWKLAQFAETIHVKFEYRGFVANTLSDLDASMLDLR--EGET 543
            LTGIGPP+HDNTD LQEVGWKLAQ AETIHV+FEYRGFVAN+L+DLDASML+LR  E E+
Sbjct: 365  LTGIGPPAHDNTDQLQEVGWKLAQLAETIHVEFEYRGFVANSLADLDASMLELRPTEFES 424

Query: 542  VAVNSVFDLHQLLARPGAIEKVMSAVADMKPEIVTIVEQEANHNGPVFLDRFTESLHYYS 363
            VAVNS+F+ H+LLA PGA++KV+S V  MKPEIVT+VEQEANHNGPVFLDRFTESLHYYS
Sbjct: 425  VAVNSIFEFHKLLAIPGAMKKVLSVVKQMKPEIVTVVEQEANHNGPVFLDRFTESLHYYS 484

Query: 362  TLFDSLESCGNGSGGIDAGANVSNQDKVMSEVYLGRQICNIVACEGADRIERHEPLTQWT 183
            TLFDSLE             +VS QDKVMSEVYL +QICN+VACEG  R+ERHE LTQW 
Sbjct: 485  TLFDSLE------------GSVSTQDKVMSEVYLAKQICNVVACEGPSRVERHETLTQWR 532

Query: 182  ERFHSAGFEPVHIGSNAYKQASMLLALFAGGNGYRVEEHEGCLMLGWHTRPLITTSAWKL 3
             R  SAGF PVH+GSNA+KQASMLLALFAGG+GYRVEE+ GCLMLGWHTRPLI TSAW++
Sbjct: 533  TRLSSAGFAPVHLGSNAFKQASMLLALFAGGDGYRVEENNGCLMLGWHTRPLIATSAWRV 592



 Score =  133 bits (335), Expect(2) = 0.0
 Identities = 75/122 (61%), Positives = 86/122 (70%), Gaps = 20/122 (16%)
 Frame = -1

Query: 1558 DELLAVLGYKVRSSDMAGVAEKLQHLEEAMGQAQQQGISHLASDTVHYNPSDLSSWLESM 1379
            DELLAVLGYKVRSSDMA VA+KL+ LEE MG AQ+ G+SHLASD+VHYNPSDLS+WLESM
Sbjct: 55   DELLAVLGYKVRSSDMAEVAQKLEQLEEVMGHAQEDGLSHLASDSVHYNPSDLSTWLESM 114

Query: 1378 IVELNPQPSDDLF-------AESSTNRII-------------FDDSCSNDYDLKAIPGKA 1259
            + ELNP    DL        AESST   I             F++S S++YDLK IPGKA
Sbjct: 115  LSELNPNHHFDLSADSLLAPAESSTVTSIDFTDRKHHQQPKLFEESSSSEYDLKVIPGKA 174

Query: 1258 VF 1253
            VF
Sbjct: 175  VF 176


>gb|ABG26370.1| DELLA protein GAI [Gossypium barbadense]
          Length = 616

 Score =  628 bits (1620), Expect(2) = 0.0
 Identities = 321/436 (73%), Positives = 356/436 (81%), Gaps = 12/436 (2%)
 Frame = -3

Query: 1274 DSGKSCFPGSTCE*KRLKTVVSPAVESP----------ASWQVPAAESQPVPVLVDSQEN 1125
            DS  S  P +    KR K+   P   S           AS+ +P A ++PV VLVDSQEN
Sbjct: 185  DSSSSSTPTNVKSEKRFKSTSGPPSPSDIFPPPPPPAAASYGIPTASTRPV-VLVDSQEN 243

Query: 1124 GVRLVHTLMACAQAVQENNLKLAEALVKQIAFLAVSQIGAMKKVATYFAEALARRIYKLY 945
            G+RLVH LMACA+AVQ+NNL LAEALVKQI FLA+SQ GAM+KVATYFAEALARRIY+ Y
Sbjct: 244  GIRLVHALMACAEAVQQNNLNLAEALVKQIGFLAISQAGAMRKVATYFAEALARRIYRFY 303

Query: 944  PQSPQDTSFNDMLEMHFYETCPYLKFAHFTANQAILEAFADKKMVHVIDFSMKEGMQWPA 765
            PQ+P D SF+D+L MHFYETCPYLKFAHFTANQAILEAF  KK VHVIDFSM +GMQWPA
Sbjct: 304  PQNPLDHSFSDVLHMHFYETCPYLKFAHFTANQAILEAFEGKKRVHVIDFSMNQGMQWPA 363

Query: 764  LMQALALRPSGPPTFRLTGIGPPSHDNTDHLQEVGWKLAQFAETIHVKFEYRGFVANTLS 585
            LMQALALR  GPP FRLTG GPPSHDN+DHLQEVG KLAQFA+ IHV+FEYRGFVAN+L+
Sbjct: 364  LMQALALRVGGPPAFRLTGFGPPSHDNSDHLQEVGCKLAQFAKKIHVEFEYRGFVANSLA 423

Query: 584  DLDASMLDLR--EGETVAVNSVFDLHQLLARPGAIEKVMSAVADMKPEIVTIVEQEANHN 411
            DLDASMLDLR  E E VAVNSVF+LH+LLARPGAI+KV S V  MKPE+VTIVEQEANHN
Sbjct: 424  DLDASMLDLRPSEVEAVAVNSVFELHKLLARPGAIDKVFSVVKQMKPELVTIVEQEANHN 483

Query: 410  GPVFLDRFTESLHYYSTLFDSLESCGNGSGGIDAGANVSNQDKVMSEVYLGRQICNIVAC 231
            GPVFLDRFTESLH+YSTLFDSLE             +VS+QDKVMSEVYLG+QICN+VAC
Sbjct: 484  GPVFLDRFTESLHFYSTLFDSLE------------GSVSSQDKVMSEVYLGKQICNVVAC 531

Query: 230  EGADRIERHEPLTQWTERFHSAGFEPVHIGSNAYKQASMLLALFAGGNGYRVEEHEGCLM 51
            EG DRIERHE LTQW  R  +AGF PVH+GSNA+KQASMLLALFAGG+GY VEE+ GCLM
Sbjct: 532  EGVDRIERHESLTQWRNRLSTAGFSPVHLGSNAFKQASMLLALFAGGDGYGVEENNGCLM 591

Query: 50   LGWHTRPLITTSAWKL 3
            LGWH RPLITTSAWKL
Sbjct: 592  LGWHNRPLITTSAWKL 607



 Score =  122 bits (306), Expect(2) = 0.0
 Identities = 70/135 (51%), Positives = 85/135 (62%), Gaps = 33/135 (24%)
 Frame = -1

Query: 1558 DELLAVLGYKVRSSDMAGVAEKLQHLEEAMGQAQQQGISHLASDTVHYNPSDLSSWLESM 1379
            DELLAVLGYKV++SDMA VA+KL+ LEE M   Q  GISHLAS+TVHYNPSDLS+WLESM
Sbjct: 41   DELLAVLGYKVKTSDMAEVAQKLEQLEEVMCNVQDDGISHLASETVHYNPSDLSTWLESM 100

Query: 1378 IVELNPQPSDDLF-----------------AESST----------------NRIIFDDSC 1298
            + ELNP  + D F                 AESST                 + IF+++ 
Sbjct: 101  LSELNPPSTFDPFGAAGTAAAALDDSFLVPAESSTLTTLDFDNINRKHQKSGQQIFEEAS 160

Query: 1297 SNDYDLKAIPGKAVF 1253
             +DYDLKAIPGKA++
Sbjct: 161  CSDYDLKAIPGKAIY 175