BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000616
         (3124 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271879.1| PREDICTED: uncharacterized protein LOC100247...   690   0.0  
ref|XP_002316143.1| integral membrane single C2 domain protein [...   679   0.0  
ref|XP_002312239.1| integral membrane single C2 domain protein [...   673   0.0  
ref|XP_003540643.1| PREDICTED: C2 domain-containing protein At1g...   673   0.0  
ref|XP_004135352.1| PREDICTED: uncharacterized protein LOC101220...   672   0.0  

>ref|XP_002271879.1| PREDICTED: uncharacterized protein LOC100247873 [Vitis vinifera]
            gi|297738258|emb|CBI27459.3| unnamed protein product
            [Vitis vinifera]
          Length = 667

 Score =  690 bits (1781), Expect = 0.0
 Identities = 368/527 (69%), Positives = 410/527 (77%), Gaps = 5/527 (0%)
 Frame = -2

Query: 1566 KRVAFSCFVSFCRREIYIIHRRKLCITSSVSGGAKNTPVKTISGKLDGKEVSQGSVALGT 1387
            KRV  +C +   RR         + + SS S GAK   V   S + +  E SQ S     
Sbjct: 46   KRVFLACAIPSDRRR----GNFNVQLASSTSRGAKIFVVNRFSEEFNDGEGSQESSVQMG 101

Query: 1386 SKFTNFREDPIVDKLRTQLGVVHTIPLPPFNQNIXXXXXXXXXXXXXFDKVWTGTKKKKS 1207
            S+FTNF+EDPIVDKLRTQLGV+H IP PP N+NI             FDKVWT  KKKKS
Sbjct: 102  SQFTNFQEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFGFFFLIGVVFDKVWTSGKKKKS 161

Query: 1206 DNE-GRHGMWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKLWKVYKLGLENWFVGLLQPV 1030
            + E GR G+WPQVPTSFSL LEKDLQRKESVEWVNMVLGKLWKVY+ G+ENW +GLLQPV
Sbjct: 162  NIEQGRSGIWPQVPTSFSLLLEKDLQRKESVEWVNMVLGKLWKVYRGGIENWLIGLLQPV 221

Query: 1029 IDNIKKPDYVKRVEIQQFSLGDEPLSVRNVERRTSRRVNDLQYQIGLRYTGGARMLLKLS 850
            IDN+KKPDYV+RVEI+QFSLGDEPLSVRNVERRTSRR NDLQYQIGLRYTGGARMLL LS
Sbjct: 222  IDNLKKPDYVQRVEIKQFSLGDEPLSVRNVERRTSRRANDLQYQIGLRYTGGARMLLMLS 281

Query: 849  LNFXXXXXXXXXXVRDFDIDGELWVKLRLIPSEPWVGAVSWAFVSLPKIKVVLSPFRLFN 670
            L F          VRDFDIDGELWVKLRLIP+EPWVGAVSWAFVSLPKIKV LSPFRLFN
Sbjct: 282  LKFSIIPIVVPVGVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKVELSPFRLFN 341

Query: 669  VMAIPVLSKFLTKLLTEDLPRLFVRPRKSVLDFQKGKAVGPVPNDFMSEETKEEKKDCVG 490
            +MAIPVLS FL KLLTEDLPRLFVRP+K+VLDFQKGKAVGPV N  ++ E +E  +D VG
Sbjct: 342  LMAIPVLSMFLKKLLTEDLPRLFVRPKKTVLDFQKGKAVGPVEN-ALTGEMQEGNRDFVG 400

Query: 489  ELSITLVDARKLSYVFSGKTDPYVILRLGDQVIRSKKNSQTSVFGSPGKPIWNQDFSMLV 310
            ELS+TLVDARKLSYVF GKTDPYV L +GDQ IRSKKNSQT+V G PG+PIWNQDF MLV
Sbjct: 401  ELSVTLVDARKLSYVFYGKTDPYVTLSIGDQKIRSKKNSQTTVIGPPGEPIWNQDFHMLV 460

Query: 309  ENPRKQILYVQVNDALGFTDLTIGTGEVALGLLEDTVPTDKIVSLLGGWRPFGERYSGEI 130
             NPRKQ L +QV D+LGF DLTIGTGEV LG L+DTVPTD+IV L GGW  F    SGEI
Sbjct: 461  ANPRKQKLLIQVKDSLGFADLTIGTGEVDLGSLKDTVPTDRIVVLQGGWGLFRRGSSGEI 520

Query: 129  LLRLTYKAYVEDDEEENTAAKVVDTEVSDDEMFDY----AIYEQPKR 1
            LLRLTYKAYVED+E++ T A+ +DT+VSDDEM D     A +EQ +R
Sbjct: 521  LLRLTYKAYVEDEEDDKTEAESMDTDVSDDEMSDSEEVDATFEQSQR 567


>ref|XP_002316143.1| integral membrane single C2 domain protein [Populus trichocarpa]
            gi|222865183|gb|EEF02314.1| integral membrane single C2
            domain protein [Populus trichocarpa]
          Length = 669

 Score =  679 bits (1751), Expect = 0.0
 Identities = 356/502 (70%), Positives = 395/502 (78%), Gaps = 5/502 (0%)
 Frame = -2

Query: 1491 ITSSVSGGAKNTPVKTISGKLDGKEVSQGSVALGTSKFTNFREDPIVDKLRTQLGVVHTI 1312
            +   +S G K   +K IS +L+ +E+SQ       S FT F+EDPIV KLRTQLGV+H I
Sbjct: 76   VNIELSKGTKGFVLKRISNELETEELSQEH---SISNFTGFQEDPIVGKLRTQLGVIHPI 132

Query: 1311 PLPPFNQNIXXXXXXXXXXXXXFDKVWTGTKKKKSDNEGRHG-MWPQVPTSFSLFLEKDL 1135
            P PP N+NI             FDK W   KK KS+ EG+ G  WPQVPTSFSLFLEKDL
Sbjct: 133  PSPPINRNIVGLFVFFFFVGVVFDKAWNSRKKDKSNEEGKRGEAWPQVPTSFSLFLEKDL 192

Query: 1134 QRKESVEWVNMVLGKLWKVYKLGLENWFVGLLQPVIDNIKKPDYVKRVEIQQFSLGDEPL 955
            QRKESVEWVNMVLGKLWKVY+ G+ENW +GLLQPVIDN+KKPDYV+RVEI+QFSLGDEPL
Sbjct: 193  QRKESVEWVNMVLGKLWKVYRGGIENWLIGLLQPVIDNLKKPDYVERVEIKQFSLGDEPL 252

Query: 954  SVRNVERRTSRRVNDLQYQIGLRYTGGARMLLKLSLNFXXXXXXXXXXVRDFDIDGELWV 775
            SVRNVERRTSRRVNDLQYQIGLRYTGGARMLL LSL F          VRDFDIDGELWV
Sbjct: 253  SVRNVERRTSRRVNDLQYQIGLRYTGGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELWV 312

Query: 774  KLRLIPSEPWVGAVSWAFVSLPKIKVVLSPFRLFNVMAIPVLSKFLTKLLTEDLPRLFVR 595
            KLRLIP+EPWVGAVSWAFVSLPKIK  LSPFRLFN+MAIPVLS FL KLLTEDLPRLFVR
Sbjct: 313  KLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMAIPVLSMFLKKLLTEDLPRLFVR 372

Query: 594  PRKSVLDFQKGKAVGPVPNDFMSEETKEEKKDCVGELSITLVDARKLSYVFSGKTDPYVI 415
            P+K VLDFQKGKAVGPV N+  S E +E  +D VGELS+TLVDARKLSYVF GKTDPYVI
Sbjct: 373  PKKIVLDFQKGKAVGPVANE--SGEMQEGNRDFVGELSVTLVDARKLSYVFLGKTDPYVI 430

Query: 414  LRLGDQVIRSKKNSQTSVFGSPGKPIWNQDFSMLVENPRKQILYVQVNDALGFTDLTIGT 235
            L LGDQ++RSKKNSQT+V G PG+PIWNQDF MLV NPRKQ L +QV D+LGFT LTIGT
Sbjct: 431  LNLGDQIMRSKKNSQTTVIGPPGEPIWNQDFHMLVTNPRKQKLNIQVKDSLGFTGLTIGT 490

Query: 234  GEVALGLLEDTVPTDKIVSLLGGWRPFGERYSGEILLRLTYKAYVEDDEEENTAAKVVDT 55
            GEV LG L+DTVPTDKIV L GGW  F +  SGEILLRLTYKAYVED++++    + VDT
Sbjct: 491  GEVDLGSLQDTVPTDKIVVLRGGWGLFRKASSGEILLRLTYKAYVEDEDDDKNEVEHVDT 550

Query: 54   EVSDDEMFD----YAIYEQPKR 1
              SDDEM D     AIYE  +R
Sbjct: 551  GASDDEMSDSDESNAIYEPSRR 572


>ref|XP_002312239.1| integral membrane single C2 domain protein [Populus trichocarpa]
            gi|222852059|gb|EEE89606.1| integral membrane single C2
            domain protein [Populus trichocarpa]
          Length = 657

 Score =  673 bits (1737), Expect = 0.0
 Identities = 352/498 (70%), Positives = 394/498 (79%), Gaps = 5/498 (1%)
 Frame = -2

Query: 1479 VSGGAKNTPVKTISGKLDGKEVSQGSVALGTSKFTNFREDPIVDKLRTQLGVVHTIPLPP 1300
            +S G K    K I+ +L+  E+SQ       S FT F+EDPIV KLRTQLG +H IP PP
Sbjct: 78   LSKGTKRFVFKRIANELETGELSQEP---SISNFTGFQEDPIVGKLRTQLGAIHPIPSPP 134

Query: 1299 FNQNIXXXXXXXXXXXXXFDKVWTGTKKKKSDNEG-RHGMWPQVPTSFSLFLEKDLQRKE 1123
             N+NI              DKVWT  K++KS+ EG R G WPQVPTSFSLFLEKDLQRKE
Sbjct: 135  INRNIVGLFVFFFFVGVVSDKVWTSRKREKSNEEGKRAGAWPQVPTSFSLFLEKDLQRKE 194

Query: 1122 SVEWVNMVLGKLWKVYKLGLENWFVGLLQPVIDNIKKPDYVKRVEIQQFSLGDEPLSVRN 943
            SVEWVNMVLGKLWKVY+ G+ENW VGLLQPVID++KKPDYV+RVEI+QFSLGDEPLSVRN
Sbjct: 195  SVEWVNMVLGKLWKVYRGGIENWLVGLLQPVIDDLKKPDYVERVEIKQFSLGDEPLSVRN 254

Query: 942  VERRTSRRVNDLQYQIGLRYTGGARMLLKLSLNFXXXXXXXXXXVRDFDIDGELWVKLRL 763
            VERRTSRRVNDLQYQIGLRYTGGARMLL LSL F          VRDFDIDGELWVKLRL
Sbjct: 255  VERRTSRRVNDLQYQIGLRYTGGARMLLMLSLKFSIIPIMLPVSVRDFDIDGELWVKLRL 314

Query: 762  IPSEPWVGAVSWAFVSLPKIKVVLSPFRLFNVMAIPVLSKFLTKLLTEDLPRLFVRPRKS 583
            IP+EPWVGA SWAFVSLPKIK  LSPFRLFN+MAIPVLS FL KLLTEDLPRLFVRP+K 
Sbjct: 315  IPTEPWVGAASWAFVSLPKIKFELSPFRLFNLMAIPVLSLFLKKLLTEDLPRLFVRPKKI 374

Query: 582  VLDFQKGKAVGPVPNDFMSEETKEEKKDCVGELSITLVDARKLSYVFSGKTDPYVILRLG 403
            VLDFQ GKAVGPV N+  S E +E  +D VGELS+TLVDARKLSYVF GKTDPYVIL LG
Sbjct: 375  VLDFQNGKAVGPVANE--SGEMQEGNEDFVGELSVTLVDARKLSYVFFGKTDPYVILSLG 432

Query: 402  DQVIRSKKNSQTSVFGSPGKPIWNQDFSMLVENPRKQILYVQVNDALGFTDLTIGTGEVA 223
            DQ++RSKKNS+T+V G PG+PIWNQDF MLV NPRKQ L +QV D+LGFTDLT+GTGEV 
Sbjct: 433  DQIMRSKKNSRTTVIGRPGEPIWNQDFHMLVANPRKQKLNIQVKDSLGFTDLTVGTGEVD 492

Query: 222  LGLLEDTVPTDKIVSLLGGWRPFGERYSGEILLRLTYKAYVEDDEEENTAAKVVDTEVSD 43
            LG L+DTVPTDKIV+L GGW  F +  SGEILLRLTYKAYVED++++    + VDT VSD
Sbjct: 493  LGSLQDTVPTDKIVALQGGWGLFRKASSGEILLRLTYKAYVEDEDDDKYGVEPVDTGVSD 552

Query: 42   DEMFD----YAIYEQPKR 1
            DE+ D     AIYE  +R
Sbjct: 553  DELSDSDDSNAIYEPSRR 570


>ref|XP_003540643.1| PREDICTED: C2 domain-containing protein At1g53590-like [Glycine max]
          Length = 665

 Score =  673 bits (1736), Expect = 0.0
 Identities = 343/473 (72%), Positives = 384/473 (81%), Gaps = 2/473 (0%)
 Frame = -2

Query: 1440 SGKLDGKEVSQGSVALGTSKFTNFREDPIVDKLRTQLGVVHTIPLPPFNQNIXXXXXXXX 1261
            +G +D +  +   V LG S FT F EDPIVDKLRTQLGV+H IP PP N+N+        
Sbjct: 83   NGVIDSELQATPPVQLG-SNFTAFSEDPIVDKLRTQLGVIHPIPSPPINRNVVFLFVFFF 141

Query: 1260 XXXXXFDKVWTGTKKKKSDNEGR--HGMWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKL 1087
                 FDK+WT  ++ K+++E R   G+WPQVPTSFSLFLEKDLQRKESVEWVNMVLGKL
Sbjct: 142  FVGVVFDKLWTSRRRNKNNSEDRLRGGVWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKL 201

Query: 1086 WKVYKLGLENWFVGLLQPVIDNIKKPDYVKRVEIQQFSLGDEPLSVRNVERRTSRRVNDL 907
            WKVY+ G+ENW +GLLQPVIDN+KKPDYV+RVEI+QFSLGDEPLSVRNVERRTSRRVNDL
Sbjct: 202  WKVYRGGIENWIIGLLQPVIDNLKKPDYVQRVEIKQFSLGDEPLSVRNVERRTSRRVNDL 261

Query: 906  QYQIGLRYTGGARMLLKLSLNFXXXXXXXXXXVRDFDIDGELWVKLRLIPSEPWVGAVSW 727
            QYQIGLRYTGGARMLL LSL F          VRDFDIDGELWVKLRLIP+EPWVGA SW
Sbjct: 262  QYQIGLRYTGGARMLLMLSLKFGIIPIVVPVGVRDFDIDGELWVKLRLIPTEPWVGAASW 321

Query: 726  AFVSLPKIKVVLSPFRLFNVMAIPVLSKFLTKLLTEDLPRLFVRPRKSVLDFQKGKAVGP 547
            AFVSLPKIK  LSPFRLFN+MAIPVLS FLTKLLTEDLP+LFVRP+K VLDFQKGKAVGP
Sbjct: 322  AFVSLPKIKFELSPFRLFNLMAIPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGP 381

Query: 546  VPNDFMSEETKEEKKDCVGELSITLVDARKLSYVFSGKTDPYVILRLGDQVIRSKKNSQT 367
            V     S E +E  KD VGELS+TLVDARKLSY+F GKTDPYVIL LG+QVIRSKKNSQT
Sbjct: 382  VAGGVKSGEMQEGNKDSVGELSVTLVDARKLSYIFYGKTDPYVILSLGNQVIRSKKNSQT 441

Query: 366  SVFGSPGKPIWNQDFSMLVENPRKQILYVQVNDALGFTDLTIGTGEVALGLLEDTVPTDK 187
            +V G PG PIWNQDF MLV NPRKQ L++QV DALGF DLTIGTGEV LG L+DTVPTD+
Sbjct: 442  TVIGPPGMPIWNQDFHMLVSNPRKQKLFIQVKDALGFADLTIGTGEVDLGSLKDTVPTDR 501

Query: 186  IVSLLGGWRPFGERYSGEILLRLTYKAYVEDDEEENTAAKVVDTEVSDDEMFD 28
            IV L GGW   G+R SGEILLRLTYKAYVED+E++ T    + T++SDDE+ D
Sbjct: 502  IVVLQGGWGFLGKRSSGEILLRLTYKAYVEDEEDDKTEVDAIYTDISDDELSD 554


>ref|XP_004135352.1| PREDICTED: uncharacterized protein LOC101220807 [Cucumis sativus]
            gi|449503295|ref|XP_004161931.1| PREDICTED:
            uncharacterized LOC101220807 [Cucumis sativus]
          Length = 674

 Score =  672 bits (1733), Expect = 0.0
 Identities = 352/520 (67%), Positives = 400/520 (76%), Gaps = 11/520 (2%)
 Frame = -2

Query: 1563 RVAFSCFVSFCRREIYIIHRRKLCITS--------SVSGGAKNTPVKTISGKLDGKEVSQ 1408
            R  FS  ++F RR   +       +TS        S   G +N  V  IS +L+G+E SQ
Sbjct: 36   RKPFSSTLTFRRRWFLVCSLSPDGVTSNFDLEFATSARRGVRNFVVNRISNELEGEEFSQ 95

Query: 1407 --GSVALGTSKFTNFREDPIVDKLRTQLGVVHTIPLPPFNQNIXXXXXXXXXXXXXFDKV 1234
               SV +G S FT F+EDPIVDKLRTQLG +H IP PP N+NI             FDK+
Sbjct: 96   EESSVQVG-SNFTGFQEDPIVDKLRTQLGAIHPIPSPPINRNIVGLFVFFFFVGVAFDKL 154

Query: 1233 WTGTKKKKS-DNEGRHGMWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKLWKVYKLGLEN 1057
            WT  K+ KS +N+GR G WPQVPTSFS FLEKDLQRKESVEWVNMVLGKLWKVY+ G+E+
Sbjct: 155  WTFRKRSKSRNNDGRLGTWPQVPTSFSSFLEKDLQRKESVEWVNMVLGKLWKVYRPGIED 214

Query: 1056 WFVGLLQPVIDNIKKPDYVKRVEIQQFSLGDEPLSVRNVERRTSRRVNDLQYQIGLRYTG 877
            W VGLLQPVIDN+KKPDYV+RVEI+QFSLG+EPLSVRNVERRTSRR NDLQYQIGLRYTG
Sbjct: 215  WLVGLLQPVIDNLKKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRANDLQYQIGLRYTG 274

Query: 876  GARMLLKLSLNFXXXXXXXXXXVRDFDIDGELWVKLRLIPSEPWVGAVSWAFVSLPKIKV 697
            GARMLL LSL F          VRDFDIDGELWVKLRLIP+EPWVGAVSWAFVSLPKIK 
Sbjct: 275  GARMLLMLSLKFGIIPIVVPVVVRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKF 334

Query: 696  VLSPFRLFNVMAIPVLSKFLTKLLTEDLPRLFVRPRKSVLDFQKGKAVGPVPNDFMSEET 517
             LSPFRLFN+MAIPVLS FLTKLLTEDLP+LFVRP+K VLDFQKGKAVGPVP++  S   
Sbjct: 335  ELSPFRLFNLMAIPVLSMFLTKLLTEDLPKLFVRPKKIVLDFQKGKAVGPVPDEVKSGVM 394

Query: 516  KEEKKDCVGELSITLVDARKLSYVFSGKTDPYVILRLGDQVIRSKKNSQTSVFGSPGKPI 337
            +E   D VGELS+TLVDARKLSY+F GKTDPYV+L LGDQ IRSKKNSQT+V G PG+PI
Sbjct: 395  QEGNNDFVGELSVTLVDARKLSYLFYGKTDPYVVLSLGDQTIRSKKNSQTTVIGPPGEPI 454

Query: 336  WNQDFSMLVENPRKQILYVQVNDALGFTDLTIGTGEVALGLLEDTVPTDKIVSLLGGWRP 157
            WNQDF MLV NPRKQ LY+QV D+LGF DLTIG  EV LG L+DTVPTD IV L  GW  
Sbjct: 455  WNQDFHMLVANPRKQKLYIQVKDSLGFADLTIGNAEVDLGSLQDTVPTDTIVVLREGWGL 514

Query: 156  FGERYSGEILLRLTYKAYVEDDEEENTAAKVVDTEVSDDE 37
            F  R SGE+L+RLTYKAYVED+E++  A+  +D ++SDD+
Sbjct: 515  FRNRSSGEVLVRLTYKAYVEDEEDDKAASDALDIDISDDD 554