BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000587
         (1808 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABI78942.1| aspartic protease [Ipomoea batatas]                    642   0.0  
dbj|BAG16519.1| putative aspartic protease [Capsicum chinense]        644   0.0  
dbj|BAB20970.1| aspartic proteinase 2 [Nepenthes alata]               592   e-167
gb|ABG37021.1| aspartic protease [Nicotiana tabacum]                  587   e-165
ref|XP_002892661.1| aspartyl protease family protein [Arabidopsi...   583   e-164

>gb|ABI78942.1| aspartic protease [Ipomoea batatas]
          Length = 508

 Score =  642 bits (1655), Expect(2) = 0.0
 Identities = 317/457 (69%), Positives = 354/457 (77%), Gaps = 8/457 (1%)
 Frame = +2

Query: 278  LTNYLDAQYYGEIGIGSPPQKFTVIFDTGSSNLWVPSSKCYFSIACWVHPXXXXXXXXXX 457
            L NYLDAQYYGEI IGSPPQKFTVIFDTGSSNLWVPSSKCYFSIAC+ H           
Sbjct: 72   LKNYLDAQYYGEISIGSPPQKFTVIFDTGSSNLWVPSSKCYFSIACYFHSKYKSSKSSTY 131

Query: 458  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSGFLSQDNVEVGDLLIKDQVFIETTKEGS 637
                                          +SGFLSQDNV VGDL++KDQVFIETTKE S
Sbjct: 132  TKIGTSCSITYGSGS---------------ISGFLSQDNVGVGDLVVKDQVFIETTKEPS 176

Query: 638  LTFVIAQFDGILGLGFQEIAVGDVVPPWYNMVEQGLVKEQVFSFWLNRDAEAEEGGELVF 817
            LTFV+A+FDG+LGLGFQEI+V DVVP WYNMVEQGLV E VFSFWLNRD  AEEGGEL+F
Sbjct: 177  LTFVLAKFDGLLGLGFQEISVEDVVPVWYNMVEQGLVDEPVFSFWLNRDTNAEEGGELIF 236

Query: 818  GGVDPKHFKGKHTYVPITEKGYWQFDMGDFLIGNKSTGYCDGGCSAIVDSGTSLLAGPTN 997
            GGVDP HFKGKHTYVP+T+KGYWQF+MGDFLIGN STG+C+GGC+AIVDSGTSLL GPT 
Sbjct: 237  GGVDPNHFKGKHTYVPVTQKGYWQFEMGDFLIGNSSTGFCEGGCAAIVDSGTSLLTGPTT 296

Query: 998  --------VVTQINHAIGAEGIVSAECKQVVSEYGEMIWDLLVSGLGPDAVCSQIGLCFS 1153
                    +VT+INHAIGAEG+VS ECK++VS+YG MIWDLLVSG+ PD VCSQ+GLCF 
Sbjct: 297  IVTEINHAIVTEINHAIGAEGVVSTECKEIVSQYGNMIWDLLVSGVKPDEVCSQVGLCFF 356

Query: 1154 NGKDQHVSSIIKTVVEKANHRQASSNDNLECTACELLVSWVANQLKQKETKESVLNYVNE 1333
            NG      S I  VVEK N  ++SS+    CTACE+ V W+ NQLKQK  KE V +YVN+
Sbjct: 357  NGA---AGSNIGMVVEKDNEGKSSSDP--MCTACEMAVVWMQNQLKQKVVKEKVFDYVNQ 411

Query: 1334 LCESFPSPMGESAIDCNSLSKMPNITFTIGSKAFSLTPEQYILKTGEGPATICLSGFIAL 1513
            LCE  PSPMGES IDCNS+S MPN+TF I  K F LTPEQYILKTGEG ATIC+SGF+A+
Sbjct: 412  LCEKIPSPMGESTIDCNSISNMPNVTFKIADKDFVLTPEQYILKTGEGVATICVSGFLAM 471

Query: 1514 DVPPPKGPLWILGDVFMGVYHTVFDYGNLQLGFAEAA 1624
            DVP P+GPLWILGDVFMGVYHTVFDYGNLQ+GFAEAA
Sbjct: 472  DVPAPRGPLWILGDVFMGVYHTVFDYGNLQIGFAEAA 508



 Score = 60.1 bits (144), Expect(2) = 0.0
 Identities = 29/50 (58%), Positives = 38/50 (76%)
 Frame = +1

Query: 13  MGHKYLWTSLCLWAFACTILHASSDDLLRVGLKKRQLDRDSIKAARSSKV 162
           M  KYL  S+ LW  AC++L ASS+ LLRVGLKK  LD +SIKAA++++V
Sbjct: 1   MAWKYLCASILLWVIACSVLPASSEKLLRVGLKKNPLDFNSIKAAKAARV 50


>dbj|BAG16519.1| putative aspartic protease [Capsicum chinense]
          Length = 506

 Score =  644 bits (1660), Expect(2) = 0.0
 Identities = 311/449 (69%), Positives = 352/449 (78%)
 Frame = +2

Query: 278  LTNYLDAQYYGEIGIGSPPQKFTVIFDTGSSNLWVPSSKCYFSIACWVHPXXXXXXXXXX 457
            L NYLDAQYYGEIGIGSPPQKF VIFDTGSSNLWVPSS+CYFSIACW H           
Sbjct: 75   LKNYLDAQYYGEIGIGSPPQKFKVIFDTGSSNLWVPSSRCYFSIACWFH----------- 123

Query: 458  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSGFLSQDNVEVGDLLIKDQVFIETTKEGS 637
                                          +SG  SQDNV+VGDL++KDQVFIE T+E S
Sbjct: 124  ----HKYKAGKSSTYTRNGKSCSIRYGTGSISGHFSQDNVQVGDLVVKDQVFIEATREPS 179

Query: 638  LTFVIAQFDGILGLGFQEIAVGDVVPPWYNMVEQGLVKEQVFSFWLNRDAEAEEGGELVF 817
            +TF+I +FDGILGLGFQEI+VG+  P WYNMV+QGLVKE VFSFW NRDA  +EGGELVF
Sbjct: 180  ITFIIGKFDGILGLGFQEISVGNATPVWYNMVDQGLVKEPVFSFWFNRDASTKEGGELVF 239

Query: 818  GGVDPKHFKGKHTYVPITEKGYWQFDMGDFLIGNKSTGYCDGGCSAIVDSGTSLLAGPTN 997
            GGVDPKHFKG HTYVP+T+KGYWQF+MGDFLIGN STGYC GGC+AIVDSGTSLLAGPT 
Sbjct: 240  GGVDPKHFKGNHTYVPLTQKGYWQFNMGDFLIGNTSTGYCAGGCAAIVDSGTSLLAGPTT 299

Query: 998  VVTQINHAIGAEGIVSAECKQVVSEYGEMIWDLLVSGLGPDAVCSQIGLCFSNGKDQHVS 1177
            +VTQ+NHAIGAEG+VSAECK +VS+YGE++WDLLVSG+ PD VCSQ GLCF NG  +HVS
Sbjct: 300  IVTQLNHAIGAEGVVSAECKTIVSQYGEVLWDLLVSGVRPDQVCSQAGLCFFNGA-EHVS 358

Query: 1178 SIIKTVVEKANHRQASSNDNLECTACELLVSWVANQLKQKETKESVLNYVNELCESFPSP 1357
            S I+TVVE+ N   +     L CT CE+ V W+ NQLKQ+ TKE VL YV++LCE  PSP
Sbjct: 359  SNIRTVVERENEGSSVGEAPL-CTVCEMAVVWIQNQLKQQGTKERVLEYVDQLCEKLPSP 417

Query: 1358 MGESAIDCNSLSKMPNITFTIGSKAFSLTPEQYILKTGEGPATICLSGFIALDVPPPKGP 1537
            MGES +DCNS+S +PNITFTI  KAF LTPEQYILKTGEG A+IC+SGF A DVPPP+GP
Sbjct: 418  MGESVVDCNSISSLPNITFTIKDKAFVLTPEQYILKTGEGIASICISGFAAFDVPPPRGP 477

Query: 1538 LWILGDVFMGVYHTVFDYGNLQLGFAEAA 1624
            LWILGDVFMG YHTVFDYGN Q+GFAEAA
Sbjct: 478  LWILGDVFMGPYHTVFDYGNSQVGFAEAA 506



 Score = 52.8 bits (125), Expect(2) = 0.0
 Identities = 26/50 (52%), Positives = 38/50 (76%)
 Frame = +1

Query: 13  MGHKYLWTSLCLWAFACTILHASSDDLLRVGLKKRQLDRDSIKAARSSKV 162
           M +K+L  +L L A AC++L ASSD+LLR+GLKK  +D +SI AAR +++
Sbjct: 1   MENKHLCAALLLLAIACSVLPASSDNLLRIGLKKHHVDVNSINAARVARL 50


>dbj|BAB20970.1| aspartic proteinase 2 [Nepenthes alata]
          Length = 514

 Score =  592 bits (1527), Expect = e-167
 Identities = 282/449 (62%), Positives = 338/449 (75%)
 Frame = +2

Query: 278  LTNYLDAQYYGEIGIGSPPQKFTVIFDTGSSNLWVPSSKCYFSIACWVHPXXXXXXXXXX 457
            L NY+DAQY+GEIGIGSPPQKFTVIFDTGSSNLWVPS+KCYFSIAC++HP          
Sbjct: 82   LKNYMDAQYFGEIGIGSPPQKFTVIFDTGSSNLWVPSAKCYFSIACYLHPKYKSFKSSTY 141

Query: 458  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSGFLSQDNVEVGDLLIKDQVFIETTKEGS 637
                                          +SGF SQD+V++GDL++++Q FIE TKE S
Sbjct: 142  AKNGKSAAIHYGTGA---------------ISGFFSQDHVKMGDLVVENQDFIEATKEPS 186

Query: 638  LTFVIAQFDGILGLGFQEIAVGDVVPPWYNMVEQGLVKEQVFSFWLNRDAEAEEGGELVF 817
            +TFV A+FDGILGLGFQEI+VGD VP WYNM++QGLV E VFSFWLNR +E EEGGE+VF
Sbjct: 187  ITFVAAKFDGILGLGFQEISVGDAVPAWYNMIDQGLVNEPVFSFWLNRKSEEEEGGEIVF 246

Query: 818  GGVDPKHFKGKHTYVPITEKGYWQFDMGDFLIGNKSTGYCDGGCSAIVDSGTSLLAGPTN 997
            GGVDP H+KG+HTYVP+T KGYWQFDM D L+G ++TGYC GGCSAI DSGTSLLAGPT 
Sbjct: 247  GGVDPNHYKGEHTYVPVTRKGYWQFDMDDVLVGGETTGYCSGGCSAIADSGTSLLAGPTT 306

Query: 998  VVTQINHAIGAEGIVSAECKQVVSEYGEMIWDLLVSGLGPDAVCSQIGLCFSNGKDQHVS 1177
            ++ QINHAIGA G+VS ECK VVS+YG+ I D LV+   P  +CSQIGLC  +GK + VS
Sbjct: 307  IIVQINHAIGASGLVSQECKAVVSQYGKAILDALVAEAQPQKICSQIGLCTFDGK-RGVS 365

Query: 1178 SIIKTVVEKANHRQASSNDNLECTACELLVSWVANQLKQKETKESVLNYVNELCESFPSP 1357
              I++VVEK     +    +  CTACE+ V W+ NQL+Q  T+E +LNYVNELC   PSP
Sbjct: 366  MGIESVVEKNPGNSSDGLQDAMCTACEMAVVWMQNQLRQNRTEEQILNYVNELCNRLPSP 425

Query: 1358 MGESAIDCNSLSKMPNITFTIGSKAFSLTPEQYILKTGEGPATICLSGFIALDVPPPKGP 1537
            MGES++DC SLS MPN++ TIG K F L+PE+Y+LK GEG A  C+SGFIALD+ PP+GP
Sbjct: 426  MGESSVDCGSLSSMPNVSLTIGGKVFDLSPEKYVLKVGEGVAAQCISGFIALDIAPPRGP 485

Query: 1538 LWILGDVFMGVYHTVFDYGNLQLGFAEAA 1624
            LWILGD+FMG YHTVFDYGNL +GFAEAA
Sbjct: 486  LWILGDIFMGQYHTVFDYGNLSVGFAEAA 514


>gb|ABG37021.1| aspartic protease [Nicotiana tabacum]
          Length = 508

 Score =  587 bits (1514), Expect = e-165
 Identities = 277/449 (61%), Positives = 339/449 (75%)
 Frame = +2

Query: 278  LTNYLDAQYYGEIGIGSPPQKFTVIFDTGSSNLWVPSSKCYFSIACWVHPXXXXXXXXXX 457
            L NYLDAQY+GEI IGSPPQKFTVIFDTGSSNLWVPS++CYFS+AC++HP          
Sbjct: 77   LKNYLDAQYFGEICIGSPPQKFTVIFDTGSSNLWVPSARCYFSLACYLHPKYKSSHSSTY 136

Query: 458  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSGFLSQDNVEVGDLLIKDQVFIETTKEGS 637
                                          +SG+ S DNV+VGDL++KDQ FIE T+E  
Sbjct: 137  KKNGTSAAIRYGTGS---------------ISGYFSNDNVKVGDLIVKDQDFIEATREPG 181

Query: 638  LTFVIAQFDGILGLGFQEIAVGDVVPPWYNMVEQGLVKEQVFSFWLNRDAEAEEGGELVF 817
            +TF+ A+FDGILGLGFQEI+VG  VP WYNMV QGLVK+ VFSFW NR+A+ EEGGELVF
Sbjct: 182  ITFLAAKFDGILGLGFQEISVGKSVPVWYNMVNQGLVKKPVFSFWFNRNAQEEEGGELVF 241

Query: 818  GGVDPKHFKGKHTYVPITEKGYWQFDMGDFLIGNKSTGYCDGGCSAIVDSGTSLLAGPTN 997
            GGVDP HFKGKHTYVP+T KGYWQFDMGD L+G ++TG+C GGCSAI DSGTSLLAGPT 
Sbjct: 242  GGVDPNHFKGKHTYVPVTHKGYWQFDMGDVLVGGETTGFCSGGCSAIADSGTSLLAGPTT 301

Query: 998  VVTQINHAIGAEGIVSAECKQVVSEYGEMIWDLLVSGLGPDAVCSQIGLCFSNGKDQHVS 1177
            ++TQINH IGA G+VS ECK +V+EYG+ I DLL S   P  +CSQIGLC S+G  + VS
Sbjct: 302  IITQINHVIGASGVVSQECKSLVTEYGKTILDLLESKAAPQKICSQIGLCSSDG-SRDVS 360

Query: 1178 SIIKTVVEKANHRQASSNDNLECTACELLVSWVANQLKQKETKESVLNYVNELCESFPSP 1357
             II++VV+K N       D + C  CE+ V W+ NQ+++ ET +S+ +YVN+LC+  PSP
Sbjct: 361  MIIESVVDKHNGASNGLGDEM-CRVCEMAVIWMQNQMRRNETADSIYDYVNQLCDRLPSP 419

Query: 1358 MGESAIDCNSLSKMPNITFTIGSKAFSLTPEQYILKTGEGPATICLSGFIALDVPPPKGP 1537
            MGESA+DC+SL+ MPN++FT+G++ F LTP+QY+L+ GEGP   C+SGF ALDVPPP+GP
Sbjct: 420  MGESAVDCSSLASMPNVSFTVGNQTFGLTPQQYVLQVGEGPVAQCISGFTALDVPPPRGP 479

Query: 1538 LWILGDVFMGVYHTVFDYGNLQLGFAEAA 1624
            LWILGDVFMG YHTVFDYGN ++GFAEAA
Sbjct: 480  LWILGDVFMGRYHTVFDYGNSRVGFAEAA 508


>ref|XP_002892661.1| aspartyl protease family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297338503|gb|EFH68920.1| aspartyl protease family
            protein [Arabidopsis lyrata subsp. lyrata]
          Length = 506

 Score =  583 bits (1502), Expect(2) = e-164
 Identities = 278/449 (61%), Positives = 333/449 (74%)
 Frame = +2

Query: 278  LTNYLDAQYYGEIGIGSPPQKFTVIFDTGSSNLWVPSSKCYFSIACWVHPXXXXXXXXXX 457
            L NYLDAQYYGEI IG+PPQKFTV+FDTGSSNLWVPSSKCYFS+AC +HP          
Sbjct: 74   LKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLACLLHPKYKSSRSSTY 133

Query: 458  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSGFLSQDNVEVGDLLIKDQVFIETTKEGS 637
                                          ++GF S D V VGDL++KDQ FIE TKE  
Sbjct: 134  EKNGKSAAIHYGTGA---------------IAGFFSNDAVTVGDLVVKDQEFIEATKEPG 178

Query: 638  LTFVIAQFDGILGLGFQEIAVGDVVPPWYNMVEQGLVKEQVFSFWLNRDAEAEEGGELVF 817
            +TFV+A+FDGILGLGFQEI+VG+  P WYNM++QGL+KE VFSFW NR+A+ EEGGELVF
Sbjct: 179  ITFVVAKFDGILGLGFQEISVGNATPVWYNMLKQGLIKEPVFSFWFNRNADEEEGGELVF 238

Query: 818  GGVDPKHFKGKHTYVPITEKGYWQFDMGDFLIGNKSTGYCDGGCSAIVDSGTSLLAGPTN 997
            GGVDP HFKGKHTYVP+T+KGYWQFDMGD LIG   TG+C+ GCSAI DSGTSLLAGPT 
Sbjct: 239  GGVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTT 298

Query: 998  VVTQINHAIGAEGIVSAECKQVVSEYGEMIWDLLVSGLGPDAVCSQIGLCFSNGKDQHVS 1177
            ++T INHAIGA G+VS +CK VV +YG+ I DLL+S   P  +CSQIGLC  +G  + VS
Sbjct: 299  IITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDG-TRGVS 357

Query: 1178 SIIKTVVEKANHRQASSNDNLECTACELLVSWVANQLKQKETKESVLNYVNELCESFPSP 1357
              I++VV+K N + ++   +  C+ACE+ V W+ +QL+Q  T+E +LNYVNELCE  PSP
Sbjct: 358  MGIESVVDKENSKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSP 417

Query: 1358 MGESAIDCNSLSKMPNITFTIGSKAFSLTPEQYILKTGEGPATICLSGFIALDVPPPKGP 1537
            MGESA+DC  LS MP ++ TIG K F L PE+Y+LK GEGP   C+SGFIALDV PP+GP
Sbjct: 418  MGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGP 477

Query: 1538 LWILGDVFMGVYHTVFDYGNLQLGFAEAA 1624
            LWILGDVFMG YHTVFD+GN Q+GFAEAA
Sbjct: 478  LWILGDVFMGKYHTVFDFGNEQVGFAEAA 506



 Score = 24.3 bits (51), Expect(2) = e-164
 Identities = 15/29 (51%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +1

Query: 82  SDDLLRVGLKKRQLDRDSIKAAR-SSKVD 165
           +D   RVGLKK +LD  +  AAR  SK D
Sbjct: 27  NDGTFRVGLKKLKLDSKNRLAARVESKQD 55