BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000584
         (957 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABG76785.1| ribosome-inactivating protein [Panax ginseng]          152   1e-56
gb|ACV60361.2| putative type 2 ribosome-inactivating protein [Ca...   109   6e-45
gb|ADF45510.1| type 2 ribosome-inactivating protein precursor [C...   109   1e-44
sp|Q9M6E9.1|AGGL_ABRPR RecName: Full=Agglutinin-1; AltName: Full...   121   3e-44
gb|ADF45512.1| type 2 ribosome-inactivating protein precursor [C...   107   3e-43

>gb|ABG76785.1| ribosome-inactivating protein [Panax ginseng]
          Length = 443

 Score =  152 bits (383), Expect(2) = 1e-56
 Identities = 95/243 (39%), Positives = 135/243 (55%), Gaps = 31/243 (12%)
 Frame = -1

Query: 951 IIVAIDKTNTFLMAYRTEYKAFFFPDTNGSLTSSLFSGIPRYIIPFDSSIKTMEKIAGPR 772
           I +AID TN +++ YRT    ++F D+    T  +F  +P   +PF+SS   ++  AG R
Sbjct: 111 ITLAIDVTNLYVVGYRTGDLFYYFLDSAVD-TPGIFRELPHIELPFNSSYPALQHEAGDR 169

Query: 771 KEIALGMGELDKSIQYLHDNSNSNLASSLIVVIQMLAQALIYRYVENVVVEHITDDVKSA 592
             I LG+ ELD+SIQ L     +NLA   IVVIQM+++A+ +RY+EN+VV  I  D    
Sbjct: 170 VNIPLGITELDQSIQDLRHYRRTNLARPFIVVIQMVSEAVRFRYIENLVVLSIRADGSRQ 229

Query: 591 VFYPSDTMLTFENSWQRLSNRIHRSVGGVIYPAFVLPNVSEP---ITSITPNLRLNIAVI 421
            F P+ TM++ EN+W  LS  I +SV G   P+  L N  +    ++S+TP L LNIAV+
Sbjct: 230 NFRPTRTMISLENNWDTLSTSIQQSVEGRFTPSVELVNREDQSVLVSSVTPGLILNIAVM 289

Query: 420 RY-ASTPN---------------------------VREPTVRIIGRNGLCADVQDAFYDD 325
            +  + PN                           + EPT+RI GRNGLCADV+D  + D
Sbjct: 290 IFVCNNPNPNQLISPMRHVNVDHVMGINDDHICSIIPEPTIRISGRNGLCADVKDRSFHD 349

Query: 324 GNP 316
           GNP
Sbjct: 350 GNP 352



 Score = 95.1 bits (235), Expect(2) = 1e-56
 Identities = 49/85 (57%), Positives = 58/85 (68%), Gaps = 1/85 (1%)
 Frame = -3

Query: 295 CKSNSYDNQLWTLMKDGTIRSKGKCLTAYRLDIEQYLMIYNC-TTVPNYSGTKWEIQDNG 119
           CKSN  +NQLWTL  DGT RS GKC+TAY     QY+MIYNC T VP  + TKW++  NG
Sbjct: 358 CKSNEDENQLWTLKTDGTTRSNGKCMTAYGFKARQYVMIYNCATAVP--TATKWQVWGNG 415

Query: 118 NVINKESKLALTAYEGYSWSLLTLD 44
            +IN ES L LTA  G S + LT+D
Sbjct: 416 TIINPESGLVLTAGLGSSRTELTVD 440


>gb|ACV60361.2| putative type 2 ribosome-inactivating protein [Camellia sinensis]
           gi|294861494|gb|ADF45509.1| type 2 ribosome-inactivating
           protein precursor [Camellia sinensis]
          Length = 570

 Score =  109 bits (273), Expect(2) = 6e-45
 Identities = 86/246 (34%), Positives = 125/246 (50%), Gaps = 32/246 (13%)
 Frame = -1

Query: 957 ARIIVAIDKTNTFLMAYRTEYKAFFFPDTNGSLTSSLFSGIPRYIIPFDSSIKTME-KIA 781
           A I +A+D  N +++AY+   +++FF D      S+LF+   +    F  S   +E +  
Sbjct: 97  ATITLAVDVQNAYVVAYQAGDQSYFFRDAPEVAFSNLFNDTQQSTFNFTGSYVDLEGRSR 156

Query: 780 GPRKEIALGMGELDKSIQYLHDNS---NSNLASSLIVVIQMLAQALIYRYVENVVVEHIT 610
             R++I LG+  L+++I  L  +S    S +A SLIV IQM+++A  +RY+E  V + IT
Sbjct: 157 HDREDIDLGILALERAIFSLSRSSAAAESTIARSLIVCIQMVSEAARFRYIERRVRQSIT 216

Query: 609 DDVKSAVFYPSDTMLTFENSWQRLSNRIHRSVGGVIY-------PAFVLPNVSEPITSIT 451
                  F P   ML+ EN+W  LS  I +S  GV         P +   NV     SI 
Sbjct: 217 S-AGYGHFRPDAGMLSLENNWGALSYAIQQSNQGVFSSPVQLQRPDYTFFNVDSATLSIV 275

Query: 450 PNLRLNIAV--IRYAST---PNVR----------------EPTVRIIGRNGLCADVQDAF 334
            NL L + V  I +AS+   P +R                EPT+RI GRNGLC DV+D  
Sbjct: 276 RNLALMLFVCNINHASSQFSPLIRSVVAPVDDDDDTCAEPEPTIRISGRNGLCVDVRDGQ 335

Query: 333 YDDGNP 316
           Y+DGNP
Sbjct: 336 YNDGNP 341



 Score = 98.6 bits (244), Expect(2) = 6e-45
 Identities = 47/98 (47%), Positives = 62/98 (63%)
 Frame = -3

Query: 295 CKSNSYDNQLWTLMKDGTIRSKGKCLTAYRLDIEQYLMIYNCTTVPNYSGTKWEIQDNGN 116
           C+SN+  NQLWTL +DGTI+S GKCLT Y      Y+MIYNC T    + T+W++ DNG 
Sbjct: 347 CESNTDANQLWTLKRDGTIQSNGKCLTTYGYSPGDYVMIYNCDTAAT-AATRWQVWDNGT 405

Query: 115 VINKESKLALTAYEGYSWSLLTLDQKSYTSSQGWSFRN 2
           +IN ES L L+A    + + LT++   Y S QGW   N
Sbjct: 406 IINPESALVLSAESENAGTTLTMEINIYASRQGWRASN 443


>gb|ADF45510.1| type 2 ribosome-inactivating protein precursor [Camellia sinensis]
           gi|294861498|gb|ADF45511.1| type 2 ribosome-inactivating
           protein precursor [Camellia sinensis]
          Length = 570

 Score =  109 bits (272), Expect(2) = 1e-44
 Identities = 86/246 (34%), Positives = 124/246 (50%), Gaps = 32/246 (13%)
 Frame = -1

Query: 957 ARIIVAIDKTNTFLMAYRTEYKAFFFPDTNGSLTSSLFSGIPRYIIPFDSSIKTME-KIA 781
           A I +A+D  N +++AY+   +++FF D      S+LF+   +    F  S   +E +  
Sbjct: 97  ATITLAVDVQNAYVVAYQAGDQSYFFRDAPEVAFSNLFNDTQQSTFNFTGSYVDLEGRSR 156

Query: 780 GPRKEIALGMGELDKSIQYLHDNS---NSNLASSLIVVIQMLAQALIYRYVENVVVEHIT 610
             R++I LG+  L+ +I  L  +S    S +A SLIV IQM+++A  +RY+E  V + IT
Sbjct: 157 QDREDIDLGILALELAISSLRRSSAAAESTIARSLIVCIQMVSEAARFRYIERRVRQSIT 216

Query: 609 DDVKSAVFYPSDTMLTFENSWQRLSNRIHRSVGGVIY-------PAFVLPNVSEPITSIT 451
                  F P   ML+ EN+W  LS  I +S  GV         P +   NV     SI 
Sbjct: 217 S-AGYEHFRPDAGMLSLENNWGALSYAIQQSNQGVFSSPVQLQRPDYTFFNVDSATLSIV 275

Query: 450 PNLRLNIAV--IRYAST---PNVR----------------EPTVRIIGRNGLCADVQDAF 334
            NL L + V  I +AS+   P +R                EPT+RI GRNGLC DV+D  
Sbjct: 276 RNLALMLFVCNINHASSQFSPLIRSVVAPVDDDDDTCAEPEPTIRISGRNGLCVDVRDGQ 335

Query: 333 YDDGNP 316
           Y+DGNP
Sbjct: 336 YNDGNP 341



 Score = 97.8 bits (242), Expect(2) = 1e-44
 Identities = 47/98 (47%), Positives = 62/98 (63%)
 Frame = -3

Query: 295 CKSNSYDNQLWTLMKDGTIRSKGKCLTAYRLDIEQYLMIYNCTTVPNYSGTKWEIQDNGN 116
           C+SN+  NQLWTL +DGTI+S GKCLT Y      Y+MIYNC T    + T+W++ DNG 
Sbjct: 347 CESNADANQLWTLKRDGTIQSNGKCLTTYGYSPGVYVMIYNCDTAVT-AATRWQVWDNGT 405

Query: 115 VINKESKLALTAYEGYSWSLLTLDQKSYTSSQGWSFRN 2
           +IN ES L L+A    + + LT++   Y S QGW   N
Sbjct: 406 IINPESALVLSAESENAGTTLTVETNIYASRQGWRASN 443


>sp|Q9M6E9.1|AGGL_ABRPR RecName: Full=Agglutinin-1; AltName: Full=Agglutinin I; Contains:
           RecName: Full=Agglutinin-1 chain A; AltName: Full=AAG-A;
           Contains: RecName: Full=Agglutinin-1 chain B; AltName:
           Full=AAG-B; Flags: Precursor
           gi|6760352|gb|AAF28309.1|AF190173_1 preproagglutinin
           [Abrus precatorius]
          Length = 547

 Score =  121 bits (303), Expect(2) = 3e-44
 Identities = 79/239 (33%), Positives = 129/239 (53%), Gaps = 27/239 (11%)
 Frame = -1

Query: 951 IIVAIDKTNTFLMAYRTEYKAFFFPDTNGSLTSSLFSGIPRYIIPFDSSIKTMEKIA-GP 775
           I + ID TN +++AYR   ++FFF +   S ++ LF+G  +Y +PFD +   +EK A   
Sbjct: 83  IQLGIDLTNAYVVAYRAGSESFFFRNAPASASTYLFTGTQQYSLPFDGNYDDLEKWAHQS 142

Query: 774 RKEIALGMGELDKSIQYLHDNSNSN--LASSLIVVIQMLAQALIYRYVENVVVEHITDDV 601
           R+ I+LG+  L + I++L   ++ +  +A +LIV+IQM+A+A  +RYV  +VV  +++  
Sbjct: 143 RQRISLGLEALRQGIKFLRSGASDDEEIARTLIVIIQMVAEAARFRYVSKLVVISLSN-- 200

Query: 600 KSAVFYPSDTMLTFENSWQRLSNRIHRSVGG---------------VIYPAFVLPNVS-- 472
             A F P  +ML+ EN+W+ LS  +  +V                 V+  +   P+VS  
Sbjct: 201 -RAAFQPDPSMLSLENTWEPLSRAVQHTVQDTFPQNVTLINVRQERVVVSSLSHPSVSAL 259

Query: 471 -------EPITSITPNLRLNIAVIRYASTPNVREPTVRIIGRNGLCADVQDAFYDDGNP 316
                   P+ +    L +   V +     +  EPTVRI GR+GLC DV D  Y++GNP
Sbjct: 260 ALMLFVCNPLNATQSPLLIRSVVEQSKICSSHYEPTVRIGGRDGLCVDVSDNAYNNGNP 318



 Score = 84.7 bits (208), Expect(2) = 3e-44
 Identities = 44/98 (44%), Positives = 54/98 (55%)
 Frame = -3

Query: 295 CKSNSYDNQLWTLMKDGTIRSKGKCLTAYRLDIEQYLMIYNCTTVPNYSGTKWEIQDNGN 116
           CK     NQLWTL  D TIRSKGKCLT Y      Y+MIY+C++      T W+I DNG 
Sbjct: 324 CKDQLEVNQLWTLKSDKTIRSKGKCLTTYGYAPGNYVMIYDCSSAV-AEATYWDIWDNGT 382

Query: 115 VINKESKLALTAYEGYSWSLLTLDQKSYTSSQGWSFRN 2
           +IN +S L L+A        LT+ +  Y   QGW   N
Sbjct: 383 IINPKSGLVLSAESSSMGGTLTVQKNDYRMRQGWRTGN 420


>gb|ADF45512.1| type 2 ribosome-inactivating protein precursor [Camellia sinensis]
          Length = 570

 Score =  107 bits (268), Expect(2) = 3e-43
 Identities = 86/246 (34%), Positives = 124/246 (50%), Gaps = 32/246 (13%)
 Frame = -1

Query: 957 ARIIVAIDKTNTFLMAYRTEYKAFFFPDTNGSLTSSLFSGIPRYIIPFDSSIKTME-KIA 781
           A I +A+D  N +++AY+   +++FF D      S+LF+   +    F  S   +E +  
Sbjct: 97  ATITLAVDVQNAYVVAYQAGDQSYFFRDAPEVAFSNLFNDTQQSTFTFTGSYVDLEGRSH 156

Query: 780 GPRKEIALGMGELDKSIQYLHDNSN---SNLASSLIVVIQMLAQALIYRYVENVVVEHIT 610
             R++I LG+  L+ +I  L  +S+   S +A SLIV IQM+++A  +RY+E  V + IT
Sbjct: 157 QDREDIDLGILALELAILSLRRSSSAAESTIARSLIVCIQMVSEAARFRYIERRVRQSIT 216

Query: 609 DDVKSAVFYPSDTMLTFENSWQRLSNRIHRSVGGVIY-------PAFVLPNVSEPITSIT 451
                  F P   ML+ EN+W  LS  I +S  GV         P +   NV     SI 
Sbjct: 217 S-AGYEHFRPDAGMLSLENNWGALSYAIQQSNQGVFSSPVQLQRPDYTFFNVDSATLSIV 275

Query: 450 PNLRLNIAV--IRYAST---PNVR----------------EPTVRIIGRNGLCADVQDAF 334
            NL L + V  I  AS+   P +R                EPT+RI GRNGLC DV+D  
Sbjct: 276 RNLALMLFVCNINQASSQFSPLIRSVVAPVDDDDDTCAEPEPTIRISGRNGLCVDVRDGQ 335

Query: 333 YDDGNP 316
           Y+DGNP
Sbjct: 336 YNDGNP 341



 Score = 94.7 bits (234), Expect(2) = 3e-43
 Identities = 46/98 (46%), Positives = 61/98 (62%)
 Frame = -3

Query: 295 CKSNSYDNQLWTLMKDGTIRSKGKCLTAYRLDIEQYLMIYNCTTVPNYSGTKWEIQDNGN 116
           C+SN+  NQLWTL +D TI+S GKCLT Y      Y+MIYNC T    + T+W++ DNG 
Sbjct: 347 CESNTDANQLWTLKRDRTIQSNGKCLTTYGYSPGDYVMIYNCDTAVT-AATRWQVWDNGT 405

Query: 115 VINKESKLALTAYEGYSWSLLTLDQKSYTSSQGWSFRN 2
           +IN ES L L+A    + + LT++   Y S QGW   N
Sbjct: 406 IINPESALVLSAESENAGTTLTVEINIYASRQGWRASN 443