BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000527
         (1518 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002533630.1| alcohol dehydrogenase, putative [Ricinus com...   711   0.0  
gb|AFP72379.1| alcohol dehydrogenase class III [Brassica olerace...   701   0.0  
ref|NP_199207.1| alcohol dehydrogenase class-3 [Arabidopsis thal...   700   0.0  
emb|CAA57973.1| class III ADH, glutathione-dependent formaldehyd...   699   0.0  
pdb|3UKO|A Chain A, Crystal Structure Of S-Nitrosoglutathione Re...   698   0.0  

>ref|XP_002533630.1| alcohol dehydrogenase, putative [Ricinus communis]
            gi|223526488|gb|EEF28759.1| alcohol dehydrogenase,
            putative [Ricinus communis]
          Length = 379

 Score =  711 bits (1836), Expect = 0.0
 Identities = 340/377 (90%), Positives = 359/377 (95%)
 Frame = -3

Query: 1459 MATQGQVITCKAAVAYEPNKPLVIEDVQVDPPQAGEVRIQILFTALCHTDAYTWSGKDPE 1280
            MATQGQVI CKAAVAYEPNKPLVIEDVQV PPQAGEVR+QILFTALCHTDAYTWSGKDPE
Sbjct: 1    MATQGQVIICKAAVAYEPNKPLVIEDVQVAPPQAGEVRVQILFTALCHTDAYTWSGKDPE 60

Query: 1279 GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECKECKFCKSGKTNLCGKVRSAT 1100
            GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAEC++CKFCKSGKTNLCGKVR+AT
Sbjct: 61   GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRDCKFCKSGKTNLCGKVRAAT 120

Query: 1099 GVGVMMNDRKSRFSINGKPLYHFMGTSTFSQYTVLHDVSVAKIDPKAPLDKVCLLGCGVP 920
            GVGVMMNDRKSRFSINGKP+YHFMGTSTFSQYTV+HDVSVAKIDPKAPL+KVCLLGCGVP
Sbjct: 121  GVGVMMNDRKSRFSINGKPIYHFMGTSTFSQYTVVHDVSVAKIDPKAPLEKVCLLGCGVP 180

Query: 919  TGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXASRVIGVDIDNRKFERAKNFGV 740
            TGLGAVWNTAKVE GSIVAVFGLGTV            ASR+IG+DID++K++RAK+FGV
Sbjct: 181  TGLGAVWNTAKVEAGSIVAVFGLGTVGLAVAEGAKAAGASRIIGIDIDSKKYDRAKDFGV 240

Query: 739  TEFVNPKDHEKPIQQVLVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 560
            TEFVNPKDH+KPIQQV+VDLTDGGVDYSFECIGNVSVMR+ALECCHKGWGTSVIVGVAAS
Sbjct: 241  TEFVNPKDHDKPIQQVIVDLTDGGVDYSFECIGNVSVMRSALECCHKGWGTSVIVGVAAS 300

Query: 559  GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHELTLAEINK 380
            GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLV+KYMKKEIKVDEYITH LTL EINK
Sbjct: 301  GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVDKYMKKEIKVDEYITHNLTLVEINK 360

Query: 379  AFDLLHEGDCLRCVLKV 329
            AFDL+HEGDCLRCVL V
Sbjct: 361  AFDLMHEGDCLRCVLSV 377


>gb|AFP72379.1| alcohol dehydrogenase class III [Brassica oleracea var. botrytis]
          Length = 379

 Score =  701 bits (1808), Expect = 0.0
 Identities = 334/375 (89%), Positives = 354/375 (94%)
 Frame = -3

Query: 1459 MATQGQVITCKAAVAYEPNKPLVIEDVQVDPPQAGEVRIQILFTALCHTDAYTWSGKDPE 1280
            MATQGQVITCKAAVAYEPNKPLVIEDVQV PPQAGEVRI+ILFTALCHTDAYTWSGKDPE
Sbjct: 1    MATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILFTALCHTDAYTWSGKDPE 60

Query: 1279 GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECKECKFCKSGKTNLCGKVRSAT 1100
            GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAEC+ECKFCKSGKTNLCGKVRSAT
Sbjct: 61   GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECRECKFCKSGKTNLCGKVRSAT 120

Query: 1099 GVGVMMNDRKSRFSINGKPLYHFMGTSTFSQYTVLHDVSVAKIDPKAPLDKVCLLGCGVP 920
            GVGVMMNDRKSRFS++GKP+YHFMGTSTFSQYTV+HDVSVAKIDPKAPL+KVCLLGCGVP
Sbjct: 121  GVGVMMNDRKSRFSVDGKPIYHFMGTSTFSQYTVVHDVSVAKIDPKAPLEKVCLLGCGVP 180

Query: 919  TGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXASRVIGVDIDNRKFERAKNFGV 740
            TGLGAVWNTAKVEPGS VA+FGLGTV            A+R+IG+DID++K+E AK FGV
Sbjct: 181  TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGATRIIGIDIDSKKYETAKKFGV 240

Query: 739  TEFVNPKDHEKPIQQVLVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 560
             EFVNPKDH+KPIQ+V+VDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS
Sbjct: 241  NEFVNPKDHQKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 300

Query: 559  GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHELTLAEINK 380
            GQEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEYITH +TL EINK
Sbjct: 301  GQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHSMTLGEINK 360

Query: 379  AFDLLHEGDCLRCVL 335
            AFDLLH+G CLRCVL
Sbjct: 361  AFDLLHDGTCLRCVL 375


>ref|NP_199207.1| alcohol dehydrogenase class-3 [Arabidopsis thaliana]
            gi|20141281|sp|Q96533.2|ADHX_ARATH RecName: Full=Alcohol
            dehydrogenase class-3; AltName: Full=Alcohol
            dehydrogenase class-III; AltName:
            Full=Glutathione-dependent formaldehyde dehydrogenase;
            Short=FALDH; Short=FDH; Short=GSH-FDH; AltName:
            Full=S-(hydroxymethyl)glutathione dehydrogenase
            gi|9758553|dbj|BAB09054.1| alcohol dehydrogenase (EC
            1.1.1.1) class III [Arabidopsis thaliana]
            gi|14517532|gb|AAK62656.1| AT5g43940/MRH10_4 [Arabidopsis
            thaliana] gi|21592856|gb|AAM64806.1| alcohol
            dehydrogenase (EC 1.1.1.1) class III [Arabidopsis
            thaliana] gi|33589744|gb|AAQ22638.1| At5g43940/MRH10_4
            [Arabidopsis thaliana] gi|110740923|dbj|BAE98557.1|
            alcohol dehydrogenase (EC 1.1.1.1) class III [Arabidopsis
            thaliana] gi|332007651|gb|AED95034.1| alcohol
            dehydrogenase class-3 [Arabidopsis thaliana]
          Length = 379

 Score =  700 bits (1807), Expect = 0.0
 Identities = 335/375 (89%), Positives = 352/375 (93%)
 Frame = -3

Query: 1459 MATQGQVITCKAAVAYEPNKPLVIEDVQVDPPQAGEVRIQILFTALCHTDAYTWSGKDPE 1280
            MATQGQVITCKAAVAYEPNKPLVIEDVQV PPQAGEVRI+IL+TALCHTDAYTWSGKDPE
Sbjct: 1    MATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPE 60

Query: 1279 GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECKECKFCKSGKTNLCGKVRSAT 1100
            GLFPCILGHEAAGIVESVGEGVTEVQ GDHVIPCYQAEC+ECKFCKSGKTNLCGKVRSAT
Sbjct: 61   GLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSAT 120

Query: 1099 GVGVMMNDRKSRFSINGKPLYHFMGTSTFSQYTVLHDVSVAKIDPKAPLDKVCLLGCGVP 920
            GVG+MMNDRKSRFS+NGKP+YHFMGTSTFSQYTV+HDVSVAKIDP APLDKVCLLGCGVP
Sbjct: 121  GVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 919  TGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXASRVIGVDIDNRKFERAKNFGV 740
            TGLGAVWNTAKVEPGS VA+FGLGTV            ASR+IG+DID++K+E AK FGV
Sbjct: 181  TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGV 240

Query: 739  TEFVNPKDHEKPIQQVLVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 560
             EFVNPKDH+KPIQ+V+VDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS
Sbjct: 241  NEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 300

Query: 559  GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHELTLAEINK 380
            GQEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEYITH LTL EINK
Sbjct: 301  GQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLTLGEINK 360

Query: 379  AFDLLHEGDCLRCVL 335
            AFDLLHEG CLRCVL
Sbjct: 361  AFDLLHEGTCLRCVL 375


>emb|CAA57973.1| class III ADH, glutathione-dependent formaldehyde dehydrogenase
            [Arabidopsis thaliana]
          Length = 379

 Score =  699 bits (1803), Expect = 0.0
 Identities = 334/375 (89%), Positives = 352/375 (93%)
 Frame = -3

Query: 1459 MATQGQVITCKAAVAYEPNKPLVIEDVQVDPPQAGEVRIQILFTALCHTDAYTWSGKDPE 1280
            MATQGQVITCKAAVAYEPNKPLVIEDVQV PPQAGEVRI+IL+TALCHTDAYTWSGKDPE
Sbjct: 1    MATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPE 60

Query: 1279 GLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECKECKFCKSGKTNLCGKVRSAT 1100
            GLFPCILGHEAAGIVESVGEGVTEVQ GDHVIPCYQAEC+ECKFCKSGKTNLCGKVRSAT
Sbjct: 61   GLFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSAT 120

Query: 1099 GVGVMMNDRKSRFSINGKPLYHFMGTSTFSQYTVLHDVSVAKIDPKAPLDKVCLLGCGVP 920
            GVG+MMNDRKSRFS+NGKP+YHFMGTSTFSQYTV+HDVSVAKIDP APLDKVCLLGCGVP
Sbjct: 121  GVGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVP 180

Query: 919  TGLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXASRVIGVDIDNRKFERAKNFGV 740
            TGLGAVWNTAKVEPGS VA+FGLGTV            ASR+IG+DID++K+E AK FGV
Sbjct: 181  TGLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGV 240

Query: 739  TEFVNPKDHEKPIQQVLVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 560
             EFVNPKDH+KPIQ+V+VDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS
Sbjct: 241  NEFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAAS 300

Query: 559  GQEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHELTLAEINK 380
            GQEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEYITH L+L EINK
Sbjct: 301  GQEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLSLGEINK 360

Query: 379  AFDLLHEGDCLRCVL 335
            AFDLLHEG CLRCVL
Sbjct: 361  AFDLLHEGTCLRCVL 375


>pdb|3UKO|A Chain A, Crystal Structure Of S-Nitrosoglutathione Reductase From
            Arabidopsis Thaliana, Complex With Nadh
            gi|414145343|pdb|3UKO|B Chain B, Crystal Structure Of
            S-Nitrosoglutathione Reductase From Arabidopsis Thaliana,
            Complex With Nadh gi|453056117|pdb|4GL4|A Chain A,
            Crystal Structure Of Oxidized S-nitrosoglutathione
            Reductase From Arabidopsis Thalina, Complex With Nadh
            gi|453056118|pdb|4GL4|B Chain B, Crystal Structure Of
            Oxidized S-nitrosoglutathione Reductase From Arabidopsis
            Thalina, Complex With Nadh
          Length = 378

 Score =  698 bits (1802), Expect = 0.0
 Identities = 334/374 (89%), Positives = 351/374 (93%)
 Frame = -3

Query: 1456 ATQGQVITCKAAVAYEPNKPLVIEDVQVDPPQAGEVRIQILFTALCHTDAYTWSGKDPEG 1277
            ATQGQVITCKAAVAYEPNKPLVIEDVQV PPQAGEVRI+IL+TALCHTDAYTWSGKDPEG
Sbjct: 1    ATQGQVITCKAAVAYEPNKPLVIEDVQVAPPQAGEVRIKILYTALCHTDAYTWSGKDPEG 60

Query: 1276 LFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECKECKFCKSGKTNLCGKVRSATG 1097
            LFPCILGHEAAGIVESVGEGVTEVQ GDHVIPCYQAEC+ECKFCKSGKTNLCGKVRSATG
Sbjct: 61   LFPCILGHEAAGIVESVGEGVTEVQAGDHVIPCYQAECRECKFCKSGKTNLCGKVRSATG 120

Query: 1096 VGVMMNDRKSRFSINGKPLYHFMGTSTFSQYTVLHDVSVAKIDPKAPLDKVCLLGCGVPT 917
            VG+MMNDRKSRFS+NGKP+YHFMGTSTFSQYTV+HDVSVAKIDP APLDKVCLLGCGVPT
Sbjct: 121  VGIMMNDRKSRFSVNGKPIYHFMGTSTFSQYTVVHDVSVAKIDPTAPLDKVCLLGCGVPT 180

Query: 916  GLGAVWNTAKVEPGSIVAVFGLGTVXXXXXXXXXXXXASRVIGVDIDNRKFERAKNFGVT 737
            GLGAVWNTAKVEPGS VA+FGLGTV            ASR+IG+DID++K+E AK FGV 
Sbjct: 181  GLGAVWNTAKVEPGSNVAIFGLGTVGLAVAEGAKTAGASRIIGIDIDSKKYETAKKFGVN 240

Query: 736  EFVNPKDHEKPIQQVLVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASG 557
            EFVNPKDH+KPIQ+V+VDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASG
Sbjct: 241  EFVNPKDHDKPIQEVIVDLTDGGVDYSFECIGNVSVMRAALECCHKGWGTSVIVGVAASG 300

Query: 556  QEISTRPFQLVTGRVWKGTAFGGFKSRSQVPWLVEKYMKKEIKVDEYITHELTLAEINKA 377
            QEISTRPFQLVTGRVWKGTAFGGFKSR+QVPWLVEKYM KEIKVDEYITH LTL EINKA
Sbjct: 301  QEISTRPFQLVTGRVWKGTAFGGFKSRTQVPWLVEKYMNKEIKVDEYITHNLTLGEINKA 360

Query: 376  FDLLHEGDCLRCVL 335
            FDLLHEG CLRCVL
Sbjct: 361  FDLLHEGTCLRCVL 374