BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000514
         (1735 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P45350.1|DRTS_DAUCA RecName: Full=Bifunctional dihydrofolate ...   649   0.0  
emb|CAA05895.1| dihydrofolate reductase-thymidylate synthetase p...   649   0.0  
ref|XP_002306118.1| dihydrofolate reductase-thymidylate synthase...   614   e-173
ref|XP_002512938.1| bifunctional dihydrofolate reductase-thymidy...   611   e-172
ref|XP_002280254.1| PREDICTED: bifunctional dihydrofolate reduct...   608   e-171

>sp|P45350.1|DRTS_DAUCA RecName: Full=Bifunctional dihydrofolate reductase-thymidylate
            synthase; Short=DHFR-TS; Includes: RecName:
            Full=Dihydrofolate reductase; Includes: RecName:
            Full=Thymidylate synthase gi|288615|emb|CAA78954.1|
            dihydrofolate reductase-thymidylate synthetase [Daucus
            carota]
          Length = 528

 Score =  649 bits (1675), Expect = 0.0
 Identities = 308/352 (87%), Positives = 326/352 (92%)
 Frame = +3

Query: 678  ELLAESPYCVSIEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPQLDESVFQ 857
            ELLA SPYCVS+EKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIP LDESVFQ
Sbjct: 119  ELLAGSPYCVSVEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQ 178

Query: 858  PWYSSFPVVENNIRYCFTTYVRVRNSDVELSSEAXXXXXXXXXXXXKFDVQKFSFLPKKV 1037
            PWYSSFP+VEN IRYCFTTYVRVRNS  EL+S+              F++Q FSFLPK V
Sbjct: 179  PWYSSFPLVENKIRYCFTTYVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTV 238

Query: 1038 FEKHEEYLYLRLVENIISNGVAKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 1217
            FEKHEEYLYL LVENIISNGV KNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV
Sbjct: 239  FEKHEEYLYLGLVENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 298

Query: 1218 VEELLWFISGSTNAKVLQEKGIHIWDGNGSRDYLDSIGLAEREEGDLGPVYGFQWRHFGA 1397
            VEELLWFISGSTNAK+L+EKG++IW+GNGSR+YLDSIGL +REEGDLGP+YGFQWRHFGA
Sbjct: 299  VEELLWFISGSTNAKILKEKGVNIWEGNGSREYLDSIGLTDREEGDLGPIYGFQWRHFGA 358

Query: 1398 RYTDMHADYTGQGFDQLLDVISKIKNTPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 1577
            RYTDMHADY+GQGFDQLLDVISKIKN PDDRRII SAWNPSDL+LMALPPCHMFAQFYVA
Sbjct: 359  RYTDMHADYSGQGFDQLLDVISKIKNNPDDRRIIQSAWNPSDLRLMALPPCHMFAQFYVA 418

Query: 1578 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFVHTIGDAH 1733
            NGELSCQMYQRSADMGLGVPFNIA+YALLTCMIAHVCDLVPGDFVH+IGDAH
Sbjct: 419  NGELSCQMYQRSADMGLGVPFNIAAYALLTCMIAHVCDLVPGDFVHSIGDAH 470



 Score =  172 bits (436), Expect = 3e-40
 Identities = 81/89 (91%), Positives = 86/89 (96%)
 Frame = +2

Query: 284 MATDTLANPTNGSGGTRPDSQRTYQVVVAATQNMGIGKDGKLPWQLPSDLKFFKDVTMTT 463
           MA++ LANPTNGSG TRPD QRTYQVVVAATQNMGIGKDGKLPW+LPSD+KFFKDVTMTT
Sbjct: 1   MASELLANPTNGSGITRPDPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMTT 60

Query: 464 SDPLKRNAVIMGRKTWESIPLQHRPLPGR 550
           SDPLKRNAVIMGRKTWESIP+QHRPLPGR
Sbjct: 61  SDPLKRNAVIMGRKTWESIPIQHRPLPGR 89


>emb|CAA05895.1| dihydrofolate reductase-thymidylate synthetase precursor [Daucus
            carota]
          Length = 577

 Score =  649 bits (1675), Expect = 0.0
 Identities = 308/352 (87%), Positives = 326/352 (92%)
 Frame = +3

Query: 678  ELLAESPYCVSIEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPQLDESVFQ 857
            ELLA SPYCVS+EKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIP LDESVFQ
Sbjct: 168  ELLAGSPYCVSVEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQ 227

Query: 858  PWYSSFPVVENNIRYCFTTYVRVRNSDVELSSEAXXXXXXXXXXXXKFDVQKFSFLPKKV 1037
            PWYSSFP+VEN IRYCFTTYVRVRNS  EL+S+              F++Q FSFLPK V
Sbjct: 228  PWYSSFPLVENKIRYCFTTYVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTV 287

Query: 1038 FEKHEEYLYLRLVENIISNGVAKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 1217
            FEKHEEYLYL LVENIISNGV KNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV
Sbjct: 288  FEKHEEYLYLGLVENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 347

Query: 1218 VEELLWFISGSTNAKVLQEKGIHIWDGNGSRDYLDSIGLAEREEGDLGPVYGFQWRHFGA 1397
            VEELLWFISGSTNAK+L+EKG++IW+GNGSR+YLDSIGL +REEGDLGP+YGFQWRHFGA
Sbjct: 348  VEELLWFISGSTNAKILKEKGVNIWEGNGSREYLDSIGLTDREEGDLGPIYGFQWRHFGA 407

Query: 1398 RYTDMHADYTGQGFDQLLDVISKIKNTPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 1577
            RYTDMHADY+GQGFDQLLDVISKIKN PDDRRII SAWNPSDL+LMALPPCHMFAQFYVA
Sbjct: 408  RYTDMHADYSGQGFDQLLDVISKIKNNPDDRRIIQSAWNPSDLRLMALPPCHMFAQFYVA 467

Query: 1578 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFVHTIGDAH 1733
            NGELSCQMYQRSADMGLGVPFNIA+YALLTCMIAHVCDLVPGDFVH+IGDAH
Sbjct: 468  NGELSCQMYQRSADMGLGVPFNIAAYALLTCMIAHVCDLVPGDFVHSIGDAH 519



 Score =  185 bits (470), Expect = 3e-44
 Identities = 96/125 (76%), Positives = 104/125 (83%)
 Frame = +2

Query: 176 LTLRSASFCSLGSRSYSCDRKLSSVNLFYFRSFTTLMATDTLANPTNGSGGTRPDSQRTY 355
           L LRSAS   L +    C+  LSS N  +   FTT MA++ LANPTNGSG TRPD QRTY
Sbjct: 20  LALRSASLYLLTN----CN--LSSSNFSHCHRFTTYMASERLANPTNGSGITRPDPQRTY 73

Query: 356 QVVVAATQNMGIGKDGKLPWQLPSDLKFFKDVTMTTSDPLKRNAVIMGRKTWESIPLQHR 535
           QVVVAATQNMGIGKDGKLPW+LPSD+KFFKDVTMTTSDPLKRNAVIMGRKTWESIP+QHR
Sbjct: 74  QVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMTTSDPLKRNAVIMGRKTWESIPIQHR 133

Query: 536 PLPGR 550
           PLPGR
Sbjct: 134 PLPGR 138


>ref|XP_002306118.1| dihydrofolate reductase-thymidylate synthase [Populus trichocarpa]
            gi|222849082|gb|EEE86629.1| dihydrofolate
            reductase-thymidylate synthase [Populus trichocarpa]
          Length = 528

 Score =  614 bits (1584), Expect = e-173
 Identities = 291/352 (82%), Positives = 318/352 (90%)
 Frame = +3

Query: 678  ELLAESPYCVSIEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPQLDESVFQ 857
            ELLA SPYC+SIEKVFVIGGGQI RE+LNAPGCDA+HITEIE   +CDTFIP +D SVFQ
Sbjct: 120  ELLAASPYCLSIEKVFVIGGGQILRESLNAPGCDAIHITEIETDFDCDTFIPAIDTSVFQ 179

Query: 858  PWYSSFPVVENNIRYCFTTYVRVRNSDVELSSEAXXXXXXXXXXXXKFDVQKFSFLPKKV 1037
            PWYSSFP VEN+IR+CFTTYVRVR+S +E  S+             KF+V+KFSFLPK V
Sbjct: 180  PWYSSFPKVENDIRHCFTTYVRVRSSTIESHSQNNGENSDGNSENSKFEVKKFSFLPKMV 239

Query: 1038 FEKHEEYLYLRLVENIISNGVAKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 1217
            FE+HEEYLYLR+V++IIS+G  K+DRT TGT+S FGCQMRFNLRK+FPLLTTKKVFWRGV
Sbjct: 240  FERHEEYLYLRMVQDIISDGNLKDDRTGTGTLSKFGCQMRFNLRKNFPLLTTKKVFWRGV 299

Query: 1218 VEELLWFISGSTNAKVLQEKGIHIWDGNGSRDYLDSIGLAEREEGDLGPVYGFQWRHFGA 1397
            VEELLWFISGST+AKVLQEKGIHIWDGN SR+YLDSIGL +REEGDLGPVYGFQWRHFGA
Sbjct: 300  VEELLWFISGSTSAKVLQEKGIHIWDGNASREYLDSIGLKDREEGDLGPVYGFQWRHFGA 359

Query: 1398 RYTDMHADYTGQGFDQLLDVISKIKNTPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 1577
            RYTDMHADYTGQGFDQLLDVI KIKN P+DRRII+SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360  RYTDMHADYTGQGFDQLLDVIDKIKNNPNDRRIIISAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 1578 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFVHTIGDAH 1733
            NGELSCQMYQRSADMGLGVPFNIASYALLTC+IAHVCDL+PGDFVH IGDAH
Sbjct: 420  NGELSCQMYQRSADMGLGVPFNIASYALLTCIIAHVCDLIPGDFVHVIGDAH 471



 Score =  137 bits (345), Expect = 9e-30
 Identities = 65/90 (72%), Positives = 80/90 (88%), Gaps = 1/90 (1%)
 Frame = +2

Query: 284 MATDTLANPTNGSGGTRP-DSQRTYQVVVAATQNMGIGKDGKLPWQLPSDLKFFKDVTMT 460
           M+ D++AN +NG+  T+  D ++TYQVVVAAT++MGIGKDGKLPW+LPSDLKFFKD+T+T
Sbjct: 1   MSGDSVANLSNGNANTQQSDPRKTYQVVVAATKDMGIGKDGKLPWKLPSDLKFFKDLTLT 60

Query: 461 TSDPLKRNAVIMGRKTWESIPLQHRPLPGR 550
           T D  K+NAVIMGRKTWESIPL+HRPLPGR
Sbjct: 61  TMDSGKKNAVIMGRKTWESIPLEHRPLPGR 90


>ref|XP_002512938.1| bifunctional dihydrofolate reductase-thymidylate synthase, putative
            [Ricinus communis] gi|223547949|gb|EEF49441.1|
            bifunctional dihydrofolate reductase-thymidylate
            synthase, putative [Ricinus communis]
          Length = 528

 Score =  611 bits (1575), Expect = e-172
 Identities = 290/352 (82%), Positives = 312/352 (88%)
 Frame = +3

Query: 678  ELLAESPYCVSIEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPQLDESVFQ 857
            ELLA SPYC+SIEKV+VIGGGQI REALNAPGCDAVHITEIE   +CDTFIP +D S+F 
Sbjct: 120  ELLAASPYCLSIEKVYVIGGGQILREALNAPGCDAVHITEIETSFDCDTFIPAIDSSIFH 179

Query: 858  PWYSSFPVVENNIRYCFTTYVRVRNSDVELSSEAXXXXXXXXXXXXKFDVQKFSFLPKKV 1037
            PWY SFP+VENNIRY F TYVRVR+S VE +               KFDV+KFSFLPK V
Sbjct: 180  PWYLSFPMVENNIRYSFATYVRVRSSAVESNGHDSGLSSESSSENSKFDVKKFSFLPKMV 239

Query: 1038 FEKHEEYLYLRLVENIISNGVAKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 1217
            F++HEE+LYLRLV+ IIS+G+ K+DRT TGT+S FGCQ+RFNLRK+FPLLTTKKVFWRGV
Sbjct: 240  FDRHEEFLYLRLVQEIISDGILKDDRTGTGTLSKFGCQVRFNLRKTFPLLTTKKVFWRGV 299

Query: 1218 VEELLWFISGSTNAKVLQEKGIHIWDGNGSRDYLDSIGLAEREEGDLGPVYGFQWRHFGA 1397
            VEELLWFISGSTNAKVLQEKGIHIWDGN SRDYLDSIGL  REEGDLGPVYGFQWRHFGA
Sbjct: 300  VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLKNREEGDLGPVYGFQWRHFGA 359

Query: 1398 RYTDMHADYTGQGFDQLLDVISKIKNTPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 1577
            RYTDMHADYTGQGFDQLLDV++KIKN PDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360  RYTDMHADYTGQGFDQLLDVVNKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 1578 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFVHTIGDAH 1733
            NGELSCQMYQRSADMGLGVPFNIASY LLTC+IAHVCDLVPGDFVH IGD H
Sbjct: 420  NGELSCQMYQRSADMGLGVPFNIASYGLLTCIIAHVCDLVPGDFVHVIGDTH 471



 Score =  139 bits (350), Expect = 2e-30
 Identities = 68/90 (75%), Positives = 78/90 (86%), Gaps = 1/90 (1%)
 Frame = +2

Query: 284 MATDTLANPTNGSGGTRP-DSQRTYQVVVAATQNMGIGKDGKLPWQLPSDLKFFKDVTMT 460
           MA DT AN +NG+      D +RTYQVVVAAT++MGIGKDGKLPW+LPSDLKFFKDVT+T
Sbjct: 1   MAGDTKANLSNGNANIEQHDPRRTYQVVVAATRDMGIGKDGKLPWRLPSDLKFFKDVTLT 60

Query: 461 TSDPLKRNAVIMGRKTWESIPLQHRPLPGR 550
           T+D  K+NAVIMGRKTWESIPL+HRPLPGR
Sbjct: 61  TTDSGKKNAVIMGRKTWESIPLEHRPLPGR 90


>ref|XP_002280254.1| PREDICTED: bifunctional dihydrofolate reductase-thymidylate
            synthase-like [Vitis vinifera]
          Length = 524

 Score =  608 bits (1568), Expect = e-171
 Identities = 291/352 (82%), Positives = 315/352 (89%)
 Frame = +3

Query: 678  ELLAESPYCVSIEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPQLDESVFQ 857
            ELLA SPYC+SIEKVFVIGGGQI REALNAPGCDA+HITEIE   ECDTFIP +D  VFQ
Sbjct: 119  ELLAASPYCLSIEKVFVIGGGQILREALNAPGCDAIHITEIETSFECDTFIPAIDSLVFQ 178

Query: 858  PWYSSFPVVENNIRYCFTTYVRVRNSDVELSSEAXXXXXXXXXXXXKFDVQKFSFLPKKV 1037
            PWYSSFP+VENNIRY F TYVRVR+S  E   +             KF+V+KFSFLPK +
Sbjct: 179  PWYSSFPLVENNIRYSFMTYVRVRSSADESQIQ---NNGDSGLESIKFEVKKFSFLPKMI 235

Query: 1038 FEKHEEYLYLRLVENIISNGVAKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGV 1217
            FE+HEEY+YLRLVE+IISNGV ++DRT TGT+S FGCQMRFNLRK+FPLLTTKKVFWRGV
Sbjct: 236  FERHEEYIYLRLVEDIISNGVQRDDRTGTGTLSKFGCQMRFNLRKTFPLLTTKKVFWRGV 295

Query: 1218 VEELLWFISGSTNAKVLQEKGIHIWDGNGSRDYLDSIGLAEREEGDLGPVYGFQWRHFGA 1397
            VEELLWFISGSTNAKVLQEKGIHIWDGN SRDYLDSIGLA+REEGDLGP+YGFQWRHFGA
Sbjct: 296  VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLADREEGDLGPIYGFQWRHFGA 355

Query: 1398 RYTDMHADYTGQGFDQLLDVISKIKNTPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 1577
             Y DMHADYTGQGFDQLLDVI+KIKN P+DRRIILSAWNPSDLKLMALPPCHMFAQFYV+
Sbjct: 356  SYIDMHADYTGQGFDQLLDVINKIKNNPNDRRIILSAWNPSDLKLMALPPCHMFAQFYVS 415

Query: 1578 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFVHTIGDAH 1733
            +GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDF+H IGD+H
Sbjct: 416  HGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLVPGDFIHVIGDSH 467



 Score =  144 bits (364), Expect = 6e-32
 Identities = 65/89 (73%), Positives = 79/89 (88%)
 Frame = +2

Query: 284 MATDTLANPTNGSGGTRPDSQRTYQVVVAATQNMGIGKDGKLPWQLPSDLKFFKDVTMTT 463
           MA+DT+ +  NG+   +PD QRTYQVVVAAT++MGIGKDGKLPW+LPSD+K FKD+TMT 
Sbjct: 1   MASDTVVSLPNGNANIQPDPQRTYQVVVAATRDMGIGKDGKLPWRLPSDMKIFKDITMTV 60

Query: 464 SDPLKRNAVIMGRKTWESIPLQHRPLPGR 550
           +D +K+NAVIMGRKTWESIPL+HRPLPGR
Sbjct: 61  ADTVKKNAVIMGRKTWESIPLEHRPLPGR 89