BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000251
         (2546 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283247.2| PREDICTED: uncharacterized protein LOC100247...   301   7e-79
ref|XP_004134381.1| PREDICTED: uncharacterized protein LOC101205...   283   2e-73
ref|XP_002304703.1| predicted protein [Populus trichocarpa] gi|2...   263   2e-67
ref|XP_004157665.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r...   261   9e-67
ref|NP_563717.1| RING/U-box domain-containing protein [Arabidops...   244   1e-61

>ref|XP_002283247.2| PREDICTED: uncharacterized protein LOC100247656 [Vitis vinifera]
          Length = 363

 Score =  301 bits (770), Expect = 7e-79
 Identities = 170/296 (57%), Positives = 198/296 (66%), Gaps = 8/296 (2%)
 Frame = +1

Query: 1402 GEPSNIRSVGTQNSHTAHSFNRIINIDEISPETRXXXXXXXXXXXXXXXDVRARQLEADE 1581
            GEPSN  S  +Q+ H       +I IDE SPE R               D RARQ+EADE
Sbjct: 69   GEPSNSGSTRSQSHHGQGVMEPVIEIDEQSPEIRHVASRNGGSMNNDDSDARARQIEADE 128

Query: 1582 VFAMELQEQLYYESNVAADREIDENVALALQQGS-LPHAFSSGTHHVSYSRSSLVPNLHR 1758
            + A ELQEQLY+E  V     ID ++A  LQQ   +    SS  H V  +    +  L+R
Sbjct: 129  ILARELQEQLYHEMPVDGGVGIDAHIAQMLQQQEQVQPTSSSRNHRVPRASGPAISRLYR 188

Query: 1759 QNQSRPSQNASNRRVAQTRNP-APRMARLRGRFPGQSRTI-SRQRNAMFPSDMDVDMRMH 1932
            Q+QSR SQN S RR  Q R P + RMA+LR RFP QS  I S +RN  FP +MD+DMR+ 
Sbjct: 189  QSQSRSSQNPSIRRGTQARGPTSTRMAQLRSRFPNQSHAIPSGERNLHFPLNMDLDMRID 248

Query: 1933 ILEALEA----FNDMGVAGNILQAQRDFNENDYEMLLALDENNHQHGGASASQINGLPES 2100
            ILEALEA    F DM + G+ILQ QRDFNENDYEMLLALDENNH + GAS +Q+N LP+S
Sbjct: 249  ILEALEAAVGDFGDMRMPGHILQIQRDFNENDYEMLLALDENNH-NVGASVNQMNSLPQS 307

Query: 2101 TVQMDNLED-CSICLETPSIGDTIRHLPCLHKFHKDCIDEWLRRKTSCPVCKSSIT 2265
            TVQ DN E+ C+ICLETP+IGDTIRHLPCLHKFHKDCID WL R TSCPVCKSSIT
Sbjct: 308  TVQTDNFEESCAICLETPTIGDTIRHLPCLHKFHKDCIDPWLARSTSCPVCKSSIT 363


>ref|XP_004134381.1| PREDICTED: uncharacterized protein LOC101205482 [Cucumis sativus]
          Length = 803

 Score =  283 bits (723), Expect = 2e-73
 Identities = 157/294 (53%), Positives = 198/294 (67%), Gaps = 6/294 (2%)
 Frame = +1

Query: 1402 GEPSNIRSVGTQNSHTAHSFNRIINIDEISPETRXXXXXXXXXXXXXXXDVRARQLEADE 1581
            GE SN RS   ++     + N +I +DE+SPE R               DVRARQLEADE
Sbjct: 513  GESSNSRSTRLKSKIVCDNLNEVIEVDELSPEMRHPVSQTGGSLNDDTSDVRARQLEADE 572

Query: 1582 VFAMELQEQLYYESNVAADREIDENVALALQQGSLPHAFSSGTHHVSYSRSSLVPNLHRQ 1761
            + A ELQEQLY E  +  + EIDE++A+ALQQ        S   H S  R SLV   +R+
Sbjct: 573  ILARELQEQLYQEIPIGGE-EIDEHLAMALQQVEHGLLAPSRRSHNS-QRGSLVAQANRR 630

Query: 1762 NQSRPSQNASNRRVAQTRNPAPRMARLRGRFPGQSRTIS-RQRNAMFPSDMDVDMRMHIL 1938
             +S+  QN SNR   +  + A RMA++R +F G S  +S RQRN  FP  MD+DMR+ IL
Sbjct: 631  TRSQSLQNPSNRTRTRVTHSA-RMAQIRNQFFGGSHRVSTRQRNLNFPMHMDLDMRLDIL 689

Query: 1939 EALEA----FNDMGVAGNILQAQRDFNENDYEMLLALDENNHQHGGASASQINGLPESTV 2106
            EALEA     +D+ +  +IL  QRDFNENDYEMLL+LDENNH+H GAS ++IN LP+STV
Sbjct: 690  EALEAAVGDMDDVRMNRDILHMQRDFNENDYEMLLSLDENNHRHAGASTNRINSLPQSTV 749

Query: 2107 QMDNLED-CSICLETPSIGDTIRHLPCLHKFHKDCIDEWLRRKTSCPVCKSSIT 2265
            Q D+ ++ C+ICL+TP+IGD IRHLPCLHKFHKDCID WL+R+TSCPVCK SIT
Sbjct: 750  QTDSTQEACAICLDTPTIGDVIRHLPCLHKFHKDCIDPWLQRRTSCPVCKCSIT 803


>ref|XP_002304703.1| predicted protein [Populus trichocarpa] gi|222842135|gb|EEE79682.1|
            predicted protein [Populus trichocarpa]
          Length = 740

 Score =  263 bits (671), Expect = 2e-67
 Identities = 191/487 (39%), Positives = 235/487 (48%), Gaps = 15/487 (3%)
 Frame = +1

Query: 850  REPKAEDMDSRRVKGKGLLTHNILSKESDAKMRPLSDRTHAGKVDISIPANEDAFEQSNR 1029
            RE  AED D  R KGK  + H   SKE DA M      T  G  D               
Sbjct: 311  REIVAEDNDCYRAKGKKAIVHPSASKEHDANM------TRDGCRDALF------------ 352

Query: 1030 ASGWISTRGRTRKLNPPLPDEEHLLFRRAGDPSNANKMPRNEVVRSNYKEGSNARKHPVS 1209
              GW ST  R++  + PL   E  +  R      +     + +V  +   G         
Sbjct: 353  -GGWRSTHKRSKTQDQPLSYMEQGILGRDDHARCSTNEHDDRLVERDSSSGGKLH----- 406

Query: 1210 SQNVSVAPITQTVSQKHQGCQLNECPGPSNTVLKRKKXXXXXXXXXXXXXXXXXXXXXXX 1389
              +V     T  ++ ++QG      P  +  +                            
Sbjct: 407  --HVGNLVATYGLTSRNQGECSTIVPDDTEVLFLGSSR---------------------- 442

Query: 1390 XXXPGEPSNIRSVGTQNSHTAHSFNRIINIDEISPETRXXXXXXXXXXXXXXXDVRARQL 1569
                 E S+ RS    N     +   I  IDE+  E R               DV ARQ+
Sbjct: 443  -----ESSSSRSSRVHNHQHDGNLEPIYEIDELLTEVRNNDPQLIGFRSNEDSDVTARQV 497

Query: 1570 EADEVFAMELQEQLYYESNVAADREIDENVALALQQ--GSLPHAFSSGTHHVSYSRSSLV 1743
            EADE+ A ELQE+LY+E       EIDEN+A  LQQ   +LP A S   H V + R+SLV
Sbjct: 498  EADEMLARELQERLYHEEPTFGGGEIDENIAWVLQQEEDALP-ATSGHNHPVPHLRNSLV 556

Query: 1744 PNLHRQNQSRPSQNASNRRVAQTRNPAPRMARLRGRFPGQSRT-ISRQRNAM-------- 1896
             +  RQ   R S N SNRR  Q +    R + LR R   ++   ISR+RN          
Sbjct: 557  AHSSRQRLPRSSHNPSNRRGNQVQVTTTRASGLRSRLSNRTPVRISRERNPFPTVFPGGL 616

Query: 1897 ---FPSDMDVDMRMHILEALEAFNDMGVAGNILQAQRDFNENDYEMLLALDENNHQHGGA 2067
               FPS MD++MR++ILE LEA      A  +L  QRDFNENDYEMLLALDENN QHG A
Sbjct: 617  NFQFPSGMDLEMRLNILENLEASM---TATRMLHVQRDFNENDYEMLLALDENNSQHG-A 672

Query: 2068 SASQINGLPESTVQMDNL-EDCSICLETPSIGDTIRHLPCLHKFHKDCIDEWLRRKTSCP 2244
            SA+QIN LPES VQ DN  E C++CLE P+IG+ IRHLPCLHKFHKDCID WL RKTSCP
Sbjct: 673  SANQINCLPESVVQTDNFGETCAVCLEAPTIGEKIRHLPCLHKFHKDCIDPWLSRKTSCP 732

Query: 2245 VCKSSIT 2265
            +CKSSIT
Sbjct: 733  ICKSSIT 739


>ref|XP_004157665.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis
            sativus]
          Length = 259

 Score =  261 bits (666), Expect = 9e-67
 Identities = 143/245 (58%), Positives = 178/245 (72%), Gaps = 6/245 (2%)
 Frame = +1

Query: 1549 DVRARQLEADEVFAMELQEQLYYESNVAADREIDENVALALQQGSLPHAFSSGTHHVSYS 1728
            DVRARQLEADE+ A ELQEQLY E  +  + EIDE++A+ALQQ        S   H S  
Sbjct: 18   DVRARQLEADEILARELQEQLYQEIPIGGE-EIDEHLAMALQQVEHGLLAPSRRSHNS-Q 75

Query: 1729 RSSLVPNLHRQNQSRPSQNASNRRVAQTRNPAPRMARLRGRFPGQSRTIS-RQRNAMFPS 1905
            R SLV   +R+ +S+  QN SNR   +  + A RMA++R +F G S  +S RQRN  FP 
Sbjct: 76   RGSLVAQANRRTRSQSLQNPSNRTRTRVTHSA-RMAQIRNQFFGGSHRVSTRQRNLNFPM 134

Query: 1906 DMDVDMRMHILEALEA----FNDMGVAGNILQAQRDFNENDYEMLLALDENNHQHGGASA 2073
             MD+DMR+ ILEALEA     +D+ +  +IL  QRDFNENDYEMLL+LDENNH+H GAS 
Sbjct: 135  HMDLDMRLDILEALEAAVGDMDDVRMNRDILHMQRDFNENDYEMLLSLDENNHRHAGAST 194

Query: 2074 SQINGLPESTVQMDNLED-CSICLETPSIGDTIRHLPCLHKFHKDCIDEWLRRKTSCPVC 2250
            ++IN LP+STVQ D+ ++ C+ICL+TP+IGD IRHLPCLHKFHKDCID WL+R+TSCPVC
Sbjct: 195  NRINSLPQSTVQTDSTQEACAICLDTPTIGDVIRHLPCLHKFHKDCIDPWLQRRTSCPVC 254

Query: 2251 KSSIT 2265
            K SIT
Sbjct: 255  KCSIT 259


>ref|NP_563717.1| RING/U-box domain-containing protein [Arabidopsis thaliana]
            gi|15450836|gb|AAK96689.1| Unknown protein [Arabidopsis
            thaliana] gi|30725482|gb|AAP37763.1| At1g04790
            [Arabidopsis thaliana] gi|332189623|gb|AEE27744.1|
            RING/U-box domain-containing protein [Arabidopsis
            thaliana]
          Length = 634

 Score =  244 bits (622), Expect = 1e-61
 Identities = 142/297 (47%), Positives = 183/297 (61%), Gaps = 9/297 (3%)
 Frame = +1

Query: 1402 GEPSNIRSVGTQNSHTAHSFNRIINIDEISPETRXXXXXXXXXXXXXXXDVRARQLEADE 1581
            GEPS+ R    QN        +++ I++ SPE R               DV  RQ+EADE
Sbjct: 340  GEPSSSRPPRIQNHLRHWHGTQVLEIEDSSPEVRVFRGPRRVENDVS--DVNIRQIEADE 397

Query: 1582 VFAMELQEQLYYESNVAADREIDENVALALQQ--GSLPHAFSSGTHHVSYSRSSLVPNLH 1755
            + A ELQEQLY E ++    +IDE +A +++Q   SL  + S  +  ++ S +++  N  
Sbjct: 398  ILARELQEQLYREESLIRHEQIDEIIARSMEQEENSLRASSSRASTRITRSSNTIAANPR 457

Query: 1756 RQN--QSRPSQNASNRRV--AQTRNPAPRMARLRG-RFPGQSRTISRQRNAMFPSDMDVD 1920
             ++  ++R  Q++S RR    Q R P    AR RG R  G S ++    N  FP DM +D
Sbjct: 458  GRSRLEARLQQHSSRRRFNPPQARAPVRAPARGRGYRLGGASASLRTALNFSFPIDMGLD 517

Query: 1921 MRMHILEALE-AFNDMGVAGNILQAQRDFNENDYEMLLALDENNHQHGGASASQINGLPE 2097
             RM ILE LE A      + N+L   RDF E+DYE+LLALDENNH+HGGASA++IN LPE
Sbjct: 518  SRMDILEELENAIGHSITSSNLLHMDRDFTEDDYELLLALDENNHRHGGASANRINNLPE 577

Query: 2098 STVQMDNLED-CSICLETPSIGDTIRHLPCLHKFHKDCIDEWLRRKTSCPVCKSSIT 2265
            STVQ DN ++ C ICLETP IGDTIRHLPCLHKFHKDCID WL R  SCPVCKSS+T
Sbjct: 578  STVQTDNFQETCVICLETPKIGDTIRHLPCLHKFHKDCIDPWLGRSKSCPVCKSSVT 634