BLASTX nr result
ID: Atropa21_contig00026139
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atropa21_contig00026139
(822 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006346019.1| PREDICTED: ent-kaur-16-ene synthase, chlorop... 422 e-116
ref|XP_004243964.1| PREDICTED: ent-kaur-16-ene synthase, chlorop... 415 e-114
gb|ACQ99376.1| ent-kaurene synthase [Coffea arabica] 290 3e-76
gb|AEZ55689.1| kaurene synthase 2, partial [Salvia miltiorrhiza] 285 2e-74
ref|XP_002311286.2| hypothetical protein POPTR_0008s08220g [Popu... 284 3e-74
gb|AEF33360.1| ent-kaurene synthase [Scoparia dulcis] 283 4e-74
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop... 281 2e-73
emb|CBI24150.3| unnamed protein product [Vitis vinifera] 281 2e-73
gb|AGN70881.1| ent-kaurene synthase [Plectranthus barbatus] 281 3e-73
dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa] 278 1e-72
gb|EOY14266.1| Ent-kaurene synthase No1, putative [Theobroma cacao] 276 5e-72
emb|CBL42916.1| kaurene synthase [Helianthus annuus] gi|29835728... 275 1e-71
emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|29835728... 269 8e-70
gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana] gi... 267 4e-69
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso... 266 7e-69
gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana] 263 6e-68
gb|EXB51253.1| Ent-kaur-16-ene synthase [Morus notabilis] 261 2e-67
ref|XP_006585394.1| PREDICTED: ent-kaur-16-ene synthase, chlorop... 261 2e-67
gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima] 257 4e-66
sp|Q39548.1|KSB_CUCMA RecName: Full=Ent-kaur-16-ene synthase, ch... 256 9e-66
>ref|XP_006346019.1| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Solanum
tuberosum]
Length = 826
Score = 422 bits (1085), Expect = e-116
Identities = 214/268 (79%), Positives = 229/268 (85%), Gaps = 14/268 (5%)
Frame = +2
Query: 59 AAASSLYLSVLKPHTIQTNTNLTNKFNTATRL--------------ACRVDATQTADFAL 196
A++SS YLSV+K TI+ LT + RL AC VDAT+ DFAL
Sbjct: 10 ASSSSSYLSVVKHQTIRDTNTLTANTIPSRRLDGGLSFTQHSHSTAACVVDATRVPDFAL 69
Query: 197 QCNETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSW 376
QCNE TKERIRKLFHKVEFSVSSYDTAWVAMVPSPHS +VPCFPECLHWVL NQLEDGSW
Sbjct: 70 QCNEATKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSAKVPCFPECLHWVLHNQLEDGSW 129
Query: 377 GLPHHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFD 556
GLPHHQP+LLKDVLSSTLACVLALKRW IGE+LIS+GLRFIELNF+SATD+DQYSPIGFD
Sbjct: 130 GLPHHQPLLLKDVLSSTLACVLALKRWGIGEQLISKGLRFIELNFASATDKDQYSPIGFD 189
Query: 557 VIFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKL 736
VIFPGMLEYAQ LSLKLHLESRVFNELLHKR I+L RSYDS SLELNAYLAYVSEGMG+L
Sbjct: 190 VIFPGMLEYAQHLSLKLHLESRVFNELLHKRAIQLTRSYDSSSLELNAYLAYVSEGMGEL 249
Query: 737 IDWKMVMKYQKKNGSLFNSPSTTAASLI 820
DWKMVMKYQ+KNGSLFNSPSTTAASLI
Sbjct: 250 QDWKMVMKYQRKNGSLFNSPSTTAASLI 277
>ref|XP_004243964.1| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Solanum
lycopersicum] gi|348659081|gb|AEP82778.1| ent-kaurene
synthase [Solanum lycopersicum]
Length = 820
Score = 415 bits (1067), Expect = e-114
Identities = 213/270 (78%), Positives = 233/270 (86%), Gaps = 11/270 (4%)
Frame = +2
Query: 44 TATIM---AAASSLYLSVLKPHTIQTNT--------NLTNKFNTATRLACRVDATQTADF 190
+ATI+ A++SS YLSV+K I+ T L+ ++++ AC VDAT+ DF
Sbjct: 2 SATIIFPAASSSSSYLSVVKHQMIRDITIPSRRLGGGLSFTQHSSSTAACVVDATRGPDF 61
Query: 191 ALQCNETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDG 370
ALQCNETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHS +VPCFPECLHWVL NQLEDG
Sbjct: 62 ALQCNETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSAKVPCFPECLHWVLHNQLEDG 121
Query: 371 SWGLPHHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIG 550
SWGLPHHQP+LLKDVLSSTLACVLALKRW IGE+LIS GLRFIELNF+SATDEDQYSPIG
Sbjct: 122 SWGLPHHQPLLLKDVLSSTLACVLALKRWGIGEQLISNGLRFIELNFASATDEDQYSPIG 181
Query: 551 FDVIFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMG 730
FDVIFPGMLEYAQ LSLKLHLES VFNELLHKR I+L R YDS SLELNAYLAYVSEG+G
Sbjct: 182 FDVIFPGMLEYAQHLSLKLHLESGVFNELLHKRAIQLTRPYDSSSLELNAYLAYVSEGIG 241
Query: 731 KLIDWKMVMKYQKKNGSLFNSPSTTAASLI 820
+L DWKMVMKYQ+KNGSLFNSPSTTAASLI
Sbjct: 242 ELQDWKMVMKYQRKNGSLFNSPSTTAASLI 271
>gb|ACQ99376.1| ent-kaurene synthase [Coffea arabica]
Length = 808
Score = 290 bits (743), Expect = 3e-76
Identities = 136/205 (66%), Positives = 172/205 (83%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
+ +KERIRKLF++V++SVS+YDTAWVAMVP PHS Q P FP+CL+W+L +QL DGSW LP
Sbjct: 52 DQSKERIRKLFNEVDYSVSAYDTAWVAMVPDPHSSQAPLFPKCLNWLLGSQLHDGSWSLP 111
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
HH P+LLKDVLSSTLACVLALK W IGEE I +G+RFIELNF+ A +E Q+SP+GFD+IF
Sbjct: 112 HHHPLLLKDVLSSTLACVLALKTWGIGEEHIKKGVRFIELNFALAYEECQFSPVGFDIIF 171
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
PGML++A++LSL LE ++ N+LLHKRD+EL R +S S + A+LAYVSEG+ KL++W
Sbjct: 172 PGMLDHARDLSLGFQLEPKLLNDLLHKRDMELGRIRESHSKKSEAFLAYVSEGLLKLLNW 231
Query: 746 KMVMKYQKKNGSLFNSPSTTAASLI 820
M MK+Q+KNGSLFNSPS TAA+ I
Sbjct: 232 DMAMKFQRKNGSLFNSPSATAAAAI 256
>gb|AEZ55689.1| kaurene synthase 2, partial [Salvia miltiorrhiza]
Length = 762
Score = 285 bits (728), Expect = 2e-74
Identities = 132/205 (64%), Positives = 165/205 (80%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
E T+ RI KLFHK E SVS+YDTAWVAMVPSP S + PCFP+CL+W+L+NQ DGSW P
Sbjct: 2 EQTRGRIAKLFHKDELSVSTYDTAWVAMVPSPTSLEEPCFPDCLNWLLENQCHDGSWARP 61
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
HH P+L KDVLSSTLAC+LALK+W +GEE I RGL F+ELNF+SATD+ Q +P+GFD+IF
Sbjct: 62 HHHPLLKKDVLSSTLACILALKKWGVGEEQIKRGLHFLELNFASATDKCQITPMGFDIIF 121
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
P ML+YA+ SL L L+ FN+L+HKRD+EL+RS + S E Y AY++EGMG+L +W
Sbjct: 122 PAMLDYARGFSLNLRLDPTTFNDLMHKRDLELKRSNRNYSSETETYWAYIAEGMGELQNW 181
Query: 746 KMVMKYQKKNGSLFNSPSTTAASLI 820
+ VMKYQ++NGSLFN PSTTAA+ I
Sbjct: 182 ESVMKYQRRNGSLFNCPSTTAAAFI 206
>ref|XP_002311286.2| hypothetical protein POPTR_0008s08220g [Populus trichocarpa]
gi|550332654|gb|EEE88653.2| hypothetical protein
POPTR_0008s08220g [Populus trichocarpa]
Length = 775
Score = 284 bits (726), Expect = 3e-74
Identities = 134/203 (66%), Positives = 160/203 (78%)
Frame = +2
Query: 212 TKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLPHH 391
TKERI+K+F K+E SVSSYDTAWVAMVPSP + PCFPEC W+L+NQL DGSW LPH
Sbjct: 39 TKERIKKMFDKIELSVSSYDTAWVAMVPSPDCPETPCFPECTKWILENQLGDGSWSLPHG 98
Query: 392 QPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIFPG 571
P+L+KD LSSTLAC+LALKRW IGEE I++GLRFIELN +S TD +Q+ PIGFD+IFPG
Sbjct: 99 NPLLVKDALSSTLACILALKRWGIGEEQINKGLRFIELNSASVTDNEQHKPIGFDIIFPG 158
Query: 572 MLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDWKM 751
M+EYA++L L L L+ N +LH+R +EL AYLAYVSEG+GKL DW+M
Sbjct: 159 MIEYAKDLDLNLPLKPTDINSMLHRRALELTSGGGKNLEGRRAYLAYVSEGIGKLQDWEM 218
Query: 752 VMKYQKKNGSLFNSPSTTAASLI 820
MKYQ+KNGSLFNSPSTTAA+ I
Sbjct: 219 AMKYQRKNGSLFNSPSTTAAAFI 241
>gb|AEF33360.1| ent-kaurene synthase [Scoparia dulcis]
Length = 790
Score = 283 bits (725), Expect = 4e-74
Identities = 130/203 (64%), Positives = 165/203 (81%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
E TKERI KL HK E S+SSYDTAWVAMVPSPHS Q PCFP+CL+W+++NQ DGSW P
Sbjct: 45 EETKERIAKLLHKAELSISSYDTAWVAMVPSPHSSQEPCFPDCLNWLMENQCPDGSWARP 104
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
HH +L KDVLSSTLA VLAL+ W +GEE ISRG+RFIE NF SA ++ Q SP+GF ++F
Sbjct: 105 HHHSLLGKDVLSSTLASVLALQGWGVGEEQISRGVRFIEQNFVSAMEKSQISPLGFGILF 164
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
PGMLEYA+++SL +H E R+ + L+H+R++E++R +S + AYLAYV+EGMGKL +W
Sbjct: 165 PGMLEYAKDISLDIHFEPRILHALMHEREMEIKRCNQIQSADTEAYLAYVAEGMGKLQNW 224
Query: 746 KMVMKYQKKNGSLFNSPSTTAAS 814
+ V+KYQ+KNGSLFNSPSTTAA+
Sbjct: 225 ESVLKYQRKNGSLFNSPSTTAAA 247
>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
vinifera]
Length = 793
Score = 281 bits (719), Expect = 2e-73
Identities = 136/204 (66%), Positives = 163/204 (79%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
E TKERI+K+F KVE SVSSYDTAWVAMVPSP+S Q P FPEC++W+L+NQ DGSWGLP
Sbjct: 55 EGTKERIKKMFDKVELSVSSYDTAWVAMVPSPYSSQAPYFPECVNWLLENQSHDGSWGLP 114
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
H P+L+KD LSSTLA VLALKRW +GEE ++GL FI NF+S +DE Q+SPIGFD+IF
Sbjct: 115 HPHPMLVKDALSSTLASVLALKRWGVGEEQRNKGLWFIASNFASVSDEKQHSPIGFDIIF 174
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
PGM+EYA+EL L L L R + +L KRD+EL+ S S + AYLAY+SEGMG+L DW
Sbjct: 175 PGMIEYAKELDLNLPLGQRDVDAMLQKRDLELKGSLGSNTKSREAYLAYISEGMGRLQDW 234
Query: 746 KMVMKYQKKNGSLFNSPSTTAASL 817
+MVMKYQ KNGSL NSPS TAA+L
Sbjct: 235 EMVMKYQMKNGSLLNSPSATAAAL 258
>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
Length = 826
Score = 281 bits (719), Expect = 2e-73
Identities = 136/204 (66%), Positives = 163/204 (79%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
E TKERI+K+F KVE SVSSYDTAWVAMVPSP+S Q P FPEC++W+L+NQ DGSWGLP
Sbjct: 80 EGTKERIKKMFDKVELSVSSYDTAWVAMVPSPYSSQAPYFPECVNWLLENQSHDGSWGLP 139
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
H P+L+KD LSSTLA VLALKRW +GEE ++GL FI NF+S +DE Q+SPIGFD+IF
Sbjct: 140 HPHPMLVKDALSSTLASVLALKRWGVGEEQRNKGLWFIASNFASVSDEKQHSPIGFDIIF 199
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
PGM+EYA+EL L L L R + +L KRD+EL+ S S + AYLAY+SEGMG+L DW
Sbjct: 200 PGMIEYAKELDLNLPLGQRDVDAMLQKRDLELKGSLGSNTKSREAYLAYISEGMGRLQDW 259
Query: 746 KMVMKYQKKNGSLFNSPSTTAASL 817
+MVMKYQ KNGSL NSPS TAA+L
Sbjct: 260 EMVMKYQMKNGSLLNSPSATAAAL 283
>gb|AGN70881.1| ent-kaurene synthase [Plectranthus barbatus]
Length = 792
Score = 281 bits (718), Expect = 3e-73
Identities = 130/203 (64%), Positives = 162/203 (79%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
E TK RI KLFHK E S+S+YDTAWVAMVPSP S + PCFP CL+W+L+NQ DGSW P
Sbjct: 50 EETKGRIAKLFHKDELSISTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCLDGSWARP 109
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
HH P+L KDVLSSTLAC+LALK+W +GEE I+RGL FIELNF+SAT++ Q +P+GFD++F
Sbjct: 110 HHHPMLKKDVLSSTLACILALKKWGVGEEQINRGLHFIELNFASATEKCQITPMGFDIVF 169
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
P ML+ A+ LSL + LE N+L++KRD+EL R Y S S E Y AY++EGMGKL +W
Sbjct: 170 PAMLDRARALSLNIRLEPTTLNDLMNKRDLELNRCYQSSSTEREVYRAYIAEGMGKLQNW 229
Query: 746 KMVMKYQKKNGSLFNSPSTTAAS 814
+ VMKYQ+KNG+LFN PSTTAA+
Sbjct: 230 ESVMKYQRKNGTLFNCPSTTAAA 252
>dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]
Length = 788
Score = 278 bits (712), Expect = 1e-72
Identities = 133/205 (64%), Positives = 166/205 (80%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
+ TKERIRKLF+ VE SVSSYDTAWVAMVPSP+S + PCFP+CL+W+L NQL+DGSWGL
Sbjct: 55 DVTKERIRKLFNNVEVSVSSYDTAWVAMVPSPNSPKSPCFPDCLNWLLDNQLDDGSWGLL 114
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
HQ L+KD LSSTLACVLALKRW++G++ I++GL +IE NF+S TD++Q SP GFD+IF
Sbjct: 115 PHQSPLIKDTLSSTLACVLALKRWNVGKDQINKGLHYIESNFASVTDKNQASPFGFDIIF 174
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
PGMLEYA++L +KL L + +LH+R++ELRR + + AYLAY+SEG+G L DW
Sbjct: 175 PGMLEYAKDLDIKLPLNQTHLSVMLHERELELRRCHSNGR---EAYLAYISEGLGNLNDW 231
Query: 746 KMVMKYQKKNGSLFNSPSTTAASLI 820
MVMKYQ KNGSLFNSPS TA+ LI
Sbjct: 232 NMVMKYQMKNGSLFNSPSATASVLI 256
>gb|EOY14266.1| Ent-kaurene synthase No1, putative [Theobroma cacao]
Length = 785
Score = 276 bits (707), Expect = 5e-72
Identities = 137/246 (55%), Positives = 177/246 (71%)
Frame = +2
Query: 80 LSVLKPHTIQTNTNLTNKFNTATRLACRVDATQTADFALQCNETTKERIRKLFHKVEFSV 259
+S+ PHT+ + ++ + + C T E TKERI+K+F +E SV
Sbjct: 1 MSLFHPHTLSFRCSTSS---ISVSMDCGAKLTTDTHTNGLNFEGTKERIKKMFDHIELSV 57
Query: 260 SSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLPHHQPVLLKDVLSSTLACV 439
SSYDTAWVAMVPSP+S PCFP CL W+L+NQL DGSWG PH P+L KD LSSTLACV
Sbjct: 58 SSYDTAWVAMVPSPNSPGNPCFPGCLQWLLENQLSDGSWGPPHRYPLLTKDSLSSTLACV 117
Query: 440 LALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIFPGMLEYAQELSLKLHLES 619
LAL+RW +G E +++GL+FIE +F S +DE+Q++P+GFD+IF GM+EYA++L+L L L S
Sbjct: 118 LALRRWGVGIEQMTKGLQFIESHFGSISDENQHTPVGFDIIFSGMIEYAKDLNLNLLLRS 177
Query: 620 RVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDWKMVMKYQKKNGSLFNSPS 799
+ + HKRD+ELR + S + AYLAYVSEG+GK DW MVMKYQ+KNGSLFNSPS
Sbjct: 178 TDVDAMFHKRDLELRSCHKESSKGMQAYLAYVSEGIGKHQDWGMVMKYQQKNGSLFNSPS 237
Query: 800 TTAASL 817
TTAA+L
Sbjct: 238 TTAAAL 243
>emb|CBL42916.1| kaurene synthase [Helianthus annuus] gi|298357286|emb|CBM82408.1|
kaurene synthase [Helianthus annuus]
Length = 774
Score = 275 bits (703), Expect = 1e-71
Identities = 133/207 (64%), Positives = 166/207 (80%), Gaps = 2/207 (0%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
+TTKERIRKLF+ V+ SVSSYDTAWVAMVPSP S + PCFPECL+W++ NQL+DGSWGL
Sbjct: 45 DTTKERIRKLFNNVDLSVSSYDTAWVAMVPSPSSPKSPCFPECLNWLVDNQLDDGSWGLV 104
Query: 386 HHQPV--LLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDV 559
+H L+KD LSSTLAC++ALKRW++GE+ I++GLRFIE N +SATD+ Q SP GFD+
Sbjct: 105 NHSSTHPLIKDSLSSTLACIVALKRWNVGEDQINKGLRFIESNLASATDKSQPSPFGFDI 164
Query: 560 IFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLI 739
IFPGMLEYAQ+L++ L + F +LH+RD+EL+R + S E+ AYLAY SEG+G L
Sbjct: 165 IFPGMLEYAQQLNINLRSKQTDFTLMLHERDLELKRCH---SNEMEAYLAYNSEGLGNLY 221
Query: 740 DWKMVMKYQKKNGSLFNSPSTTAASLI 820
DW MV KYQ KNGS+FNSPS TAA+ I
Sbjct: 222 DWHMVKKYQMKNGSVFNSPSATAAAFI 248
>emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|298357288|emb|CBM82409.1|
kaurene synthase [Helianthus annuus]
Length = 772
Score = 269 bits (688), Expect = 8e-70
Identities = 129/207 (62%), Positives = 163/207 (78%), Gaps = 2/207 (0%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
+TTKERIRKLF+ V+ SVSSYDTAWVAMVPSP S + PCFPECL+W++ NQL+DGSWGL
Sbjct: 42 DTTKERIRKLFNNVDLSVSSYDTAWVAMVPSPSSPKSPCFPECLNWLVDNQLDDGSWGLV 101
Query: 386 HHQPV--LLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDV 559
+H LLKD LSSTLAC++ALKRW++GE I++GL FIE NF+SATD+ SP GFD+
Sbjct: 102 NHSRTHPLLKDSLSSTLACIVALKRWNVGEAQINKGLHFIESNFASATDKSHPSPFGFDI 161
Query: 560 IFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLI 739
IFPGM+EYA+ L++ L F +LH+R++EL+R + S E+ AYLAY+SEG+G L
Sbjct: 162 IFPGMVEYAERLNINLRSNQTDFTLMLHERELELKRCHSS---EMKAYLAYISEGLGNLY 218
Query: 740 DWKMVMKYQKKNGSLFNSPSTTAASLI 820
DW +V KYQ KNGS+FNSPS TAA+ I
Sbjct: 219 DWHIVKKYQLKNGSVFNSPSATAAAFI 245
>gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana] gi|507118465|gb|AGM34859.1|
Ent-kaurene synthase [synthetic construct]
Length = 784
Score = 267 bits (682), Expect = 4e-69
Identities = 130/209 (62%), Positives = 164/209 (78%), Gaps = 4/209 (1%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
+TTKERI+KLF VE SVSSYDTAWVAMVPSP+S + PCFPECL+W++ NQL DGSWGL
Sbjct: 43 DTTKERIQKLFKNVEISVSSYDTAWVAMVPSPNSPKSPCFPECLNWLINNQLNDGSWGLV 102
Query: 386 HH----QPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGF 553
+H LLKD LSSTLAC++ALKRW++GE+ I++GL FIE N +SATD+ Q SPIGF
Sbjct: 103 NHTHNHNHPLLKDSLSSTLACIVALKRWNVGEDQINKGLSFIESNLASATDKSQPSPIGF 162
Query: 554 DVIFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGK 733
D+IFPG+LEYA+ L + L + F+ +LHKR++E +R + S E++ YLAY+SEG+G
Sbjct: 163 DIIFPGLLEYAKNLDINLLSKQTDFSLMLHKRELEQKRCH---SNEIDGYLAYISEGLGN 219
Query: 734 LIDWKMVMKYQKKNGSLFNSPSTTAASLI 820
L DW MV KYQ KNGS+FNSPS TAA+ I
Sbjct: 220 LYDWNMVKKYQMKNGSVFNSPSATAAAFI 248
>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
synthase B, chloroplast precursor, putative [Ricinus
communis]
Length = 808
Score = 266 bits (680), Expect = 7e-69
Identities = 123/195 (63%), Positives = 158/195 (81%), Gaps = 1/195 (0%)
Frame = +2
Query: 233 LFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLPHHQPVLLKD 412
+F K+E SVS YDTAWVAM+PS +S Q P FPEC W++ NQL DGSWGLPHH P+L+KD
Sbjct: 1 MFDKIELSVSPYDTAWVAMIPSLNSVQAPFFPECTKWIVDNQLSDGSWGLPHHHPLLIKD 60
Query: 413 VLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIFPGMLEYAQE 592
LSSTLACVLALK+W +GE L+++GL+FIELN +S DE Q++PIGFD+IFP MLE+A+E
Sbjct: 61 TLSSTLACVLALKKWGVGETLVNKGLQFIELNSTSLNDEKQHTPIGFDIIFPAMLEHAKE 120
Query: 593 LSLKLHLESRVFNELLHKRDIELRRSYDSRSLE-LNAYLAYVSEGMGKLIDWKMVMKYQK 769
L+L L L+S V + +LH+RD++L+ + E AYLAY++EG+GK DW+MVMKYQ+
Sbjct: 121 LALNLPLKSDVIDAMLHRRDVDLKSGSGGSNTEGRKAYLAYIAEGIGKFQDWEMVMKYQR 180
Query: 770 KNGSLFNSPSTTAAS 814
KNGSLFNSPSTTAA+
Sbjct: 181 KNGSLFNSPSTTAAA 195
>gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]
Length = 784
Score = 263 bits (672), Expect = 6e-68
Identities = 128/209 (61%), Positives = 163/209 (77%), Gaps = 4/209 (1%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
+TTKERI+K F VE SVSSYDTAWVAMVPSP+S + PCFPECL+W++ NQL DGSWGL
Sbjct: 43 DTTKERIQKQFKNVEISVSSYDTAWVAMVPSPNSPKSPCFPECLNWLINNQLNDGSWGLV 102
Query: 386 HH----QPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGF 553
+H LLKD LSSTLAC++ALKRW++GE+ I++GL FIE N +SAT++ Q SPIGF
Sbjct: 103 NHTHNHNHPLLKDSLSSTLACIVALKRWNVGEDQINKGLSFIESNLASATEKSQPSPIGF 162
Query: 554 DVIFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGK 733
D+IFPG+LEYA+ L + L + F+ +LHKR++E +R + S E++ YLAY+SEG+G
Sbjct: 163 DIIFPGLLEYAKNLDINLLSKQTDFSLMLHKRELEQKRCH---SNEMDGYLAYISEGLGN 219
Query: 734 LIDWKMVMKYQKKNGSLFNSPSTTAASLI 820
L DW MV KYQ KNGS+FNSPS TAA+ I
Sbjct: 220 LYDWNMVKKYQMKNGSVFNSPSATAAAFI 248
>gb|EXB51253.1| Ent-kaur-16-ene synthase [Morus notabilis]
Length = 735
Score = 261 bits (668), Expect = 2e-67
Identities = 120/194 (61%), Positives = 156/194 (80%)
Frame = +2
Query: 233 LFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLPHHQPVLLKD 412
+F+KVE SVSSYDTAWVAMVPSP S + P FP+C++W+L+NQ +G+WGLPHH +L+KD
Sbjct: 1 MFNKVELSVSSYDTAWVAMVPSPSSARDPFFPQCVNWLLENQHSNGAWGLPHHHSLLMKD 60
Query: 413 VLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIFPGMLEYAQE 592
L STLA VLALK+W++GEE I++GL FIE N +S TD+ QYSP+GF+VIFP M+EYA+
Sbjct: 61 ALLSTLASVLALKQWNVGEEQINKGLHFIEANLASVTDDKQYSPVGFEVIFPTMIEYAKN 120
Query: 593 LSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDWKMVMKYQKK 772
L L +E F+ L+HKR++EL+ +Y S S AYLAY+SEG+GK +DW+ VMKYQ+K
Sbjct: 121 LDLNFSMEPSNFDALIHKRELELKSAYGSNSEGQRAYLAYISEGIGKSVDWEAVMKYQRK 180
Query: 773 NGSLFNSPSTTAAS 814
NGSLFNSPSTTA +
Sbjct: 181 NGSLFNSPSTTAVA 194
>ref|XP_006585394.1| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like isoform X1
[Glycine max]
Length = 834
Score = 261 bits (667), Expect = 2e-67
Identities = 124/204 (60%), Positives = 155/204 (75%)
Frame = +2
Query: 200 CNETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWG 379
C+E +KERIRKLF+KVE SVSSYDTAWVAM+PSP S P FP+CL+W+L NQL DGSWG
Sbjct: 67 CHEASKERIRKLFNKVELSVSSYDTAWVAMIPSPASPHTPFFPQCLNWLLYNQLLDGSWG 126
Query: 380 LPHHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDV 559
LP P+L+ D L STLA +LALK+W +GE+ I+RGLRFI+ N +S DE+Q+ PIGF +
Sbjct: 127 LPDRHPLLMNDALLSTLASILALKQWGVGEDQINRGLRFIQSNITSINDENQHPPIGFGI 186
Query: 560 IFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLI 739
+FP M+EYAQ L + L + + ++ KR+IEL R S S AYLAYVSEGM +
Sbjct: 187 LFPSMIEYAQNLGINLPIGATSLEAMIQKREIELHRGSQSNSDGRRAYLAYVSEGMLESQ 246
Query: 740 DWKMVMKYQKKNGSLFNSPSTTAA 811
DWK +MKYQ+KNGSLFNSP+TTAA
Sbjct: 247 DWKSIMKYQRKNGSLFNSPATTAA 270
>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
Length = 784
Score = 257 bits (656), Expect = 4e-66
Identities = 119/203 (58%), Positives = 155/203 (76%)
Frame = +2
Query: 206 ETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHWVLQNQLEDGSWGLP 385
E TK+RI+ +F KVE SVSSYDTAWVAM+P ++ Q P FP+C++W+L NQL DGSWGLP
Sbjct: 38 EGTKQRIKTMFDKVELSVSSYDTAWVAMIPCQNTPQAPFFPQCVNWLLDNQLHDGSWGLP 97
Query: 386 HHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSATDEDQYSPIGFDVIF 565
+ L+KD L STLAC+L LK+W +GEE +++GL FIE N ++ATDE Q SPIGFD+IF
Sbjct: 98 NRDSFLVKDALLSTLACILPLKQWGVGEEQMNKGLFFIESNIAAATDEKQVSPIGFDIIF 157
Query: 566 PGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAYLAYVSEGMGKLIDW 745
P ++EYA+ L L + L + + L HKR++EL+R Y S +YLAY SEG+GK DW
Sbjct: 158 PALIEYAKNLDLSIPLGATNLDALFHKRELELKRGYGSNLEGKGSYLAYFSEGLGKSADW 217
Query: 746 KMVMKYQKKNGSLFNSPSTTAAS 814
+ +MKYQ+KNGSLFNSPSTTAA+
Sbjct: 218 ETIMKYQRKNGSLFNSPSTTAAA 240
>sp|Q39548.1|KSB_CUCMA RecName: Full=Ent-kaur-16-ene synthase, chloroplastic; AltName:
Full=Ent-kaurene synthase; AltName: Full=Ent-kaurene
synthase B; Short=KSB; Flags: Precursor
gi|1431870|gb|AAB39482.1| ent-kaurene synthase B
[Cucurbita maxima]
Length = 789
Score = 256 bits (653), Expect = 9e-66
Identities = 132/219 (60%), Positives = 164/219 (74%)
Frame = +2
Query: 164 VDATQTADFALQCNETTKERIRKLFHKVEFSVSSYDTAWVAMVPSPHSDQVPCFPECLHW 343
VD T E TKERI+KLF KVE SVS+YDTAWVAMVPSP+S P FPEC++W
Sbjct: 28 VDVDTTTKTGALHFEETKERIKKLFDKVELSVSAYDTAWVAMVPSPNSLNQPLFPECINW 87
Query: 344 VLQNQLEDGSWGLPHHQPVLLKDVLSSTLACVLALKRWSIGEELISRGLRFIELNFSSAT 523
VL +Q DGSWGL H+ +L+K L STLACVL LKRW+IG + +S+ L FI+ N +SAT
Sbjct: 88 VLDSQHADGSWGLLHNDQLLMKANLLSTLACVLTLKRWNIGHDHMSKALDFIKSNIASAT 147
Query: 524 DEDQYSPIGFDVIFPGMLEYAQELSLKLHLESRVFNELLHKRDIELRRSYDSRSLELNAY 703
DE+Q SP+GFD+IFPGM+EYA++L+L L L + L+ K+++EL RS S S AY
Sbjct: 148 DENQRSPVGFDIIFPGMIEYAKDLNLNLPLAPTNVDALVRKKELEL-RSCRSNSEGGKAY 206
Query: 704 LAYVSEGMGKLIDWKMVMKYQKKNGSLFNSPSTTAASLI 820
LAYVSEG+GKL DW MVM+YQ+KNGSLFNSPSTTAA+ +
Sbjct: 207 LAYVSEGIGKLQDWDMVMQYQRKNGSLFNSPSTTAAAFM 245