BLASTX nr result
ID: Atropa21_contig00019479
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atropa21_contig00019479
(975 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006354927.1| PREDICTED: nucleobase-ascorbate transporter ... 449 e-124
ref|XP_004238190.1| PREDICTED: nucleobase-ascorbate transporter ... 447 e-123
ref|XP_006473388.1| PREDICTED: nucleobase-ascorbate transporter ... 393 e-107
ref|XP_006434867.1| hypothetical protein CICLE_v10000779mg [Citr... 392 e-106
gb|EXB38630.1| Nucleobase-ascorbate transporter 3 [Morus notabilis] 389 e-106
ref|XP_004499100.1| PREDICTED: nucleobase-ascorbate transporter ... 387 e-105
ref|XP_006386511.1| PIGMENT DEFECTIVE EMBRYO 135 family protein ... 386 e-105
ref|XP_003589253.1| Nucleobase-ascorbate transporter [Medicago t... 386 e-105
ref|XP_003544440.1| PREDICTED: nucleobase-ascorbate transporter ... 384 e-104
ref|XP_002282229.1| PREDICTED: nucleobase-ascorbate transporter ... 384 e-104
gb|EOY14596.1| Xanthine/uracil permease family protein isoform 1... 383 e-104
gb|AFK46481.1| unknown [Medicago truncatula] 383 e-104
ref|XP_003550352.1| PREDICTED: nucleobase-ascorbate transporter ... 383 e-104
gb|EOY14597.1| Xanthine/uracil permease family protein isoform 2... 379 e-102
gb|ESW32808.1| hypothetical protein PHAVU_001G018700g [Phaseolus... 377 e-102
ref|XP_002510280.1| purine permease, putative [Ricinus communis]... 376 e-102
ref|XP_006375005.1| PIGMENT DEFECTIVE EMBRYO 135 family protein ... 374 e-101
gb|EMJ26942.1| hypothetical protein PRUPE_ppa003778mg [Prunus pe... 372 e-101
ref|XP_004160603.1| PREDICTED: nucleobase-ascorbate transporter ... 372 e-100
ref|XP_004141239.1| PREDICTED: nucleobase-ascorbate transporter ... 372 e-100
>ref|XP_006354927.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Solanum
tuberosum]
Length = 549
Score = 449 bits (1156), Expect = e-124
Identities = 233/270 (86%), Positives = 236/270 (87%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LLSSAPWIKVPYPFQWGTPIFRAS VFGMMGAALVSSAESTATFYAASRLAGAT PPAHV
Sbjct: 280 LLSSAPWIKVPYPFQWGTPIFRASHVFGMMGAALVSSAESTATFYAASRLAGATLPPAHV 339
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
VSRSI GAVVG TASVENVGLLGLTRVGSRRVVQISTAFM
Sbjct: 340 VSRSIGLQGIGQLFDGFFGAVVGTTASVENVGLLGLTRVGSRRVVQISTAFMIFFSIFGK 399
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
AQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF
Sbjct: 400 FGAFFAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 459
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNTDI GHGPV TP+GWFNDILNTIFSSPPTVATIVGT +DNTLEAR S DDRGVPWLV
Sbjct: 460 VMNTDITGHGPVRTPAGWFNDILNTIFSSPPTVATIVGTVVDNTLEARHSYDDRGVPWLV 519
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQRRKGDSRN+EFYSYPLRINEYIPSRFL
Sbjct: 520 PFQRRKGDSRNEEFYSYPLRINEYIPSRFL 549
>ref|XP_004238190.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Solanum
lycopersicum]
Length = 549
Score = 447 bits (1151), Expect = e-123
Identities = 229/270 (84%), Positives = 237/270 (87%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LLSSAPWIKVPYPF WGTPIFRAS+VFGMMGAALVS+AESTATFYAASRLAGAT PPAHV
Sbjct: 280 LLSSAPWIKVPYPFLWGTPIFRASNVFGMMGAALVSAAESTATFYAASRLAGATTPPAHV 339
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
VSRSI GA+VG TASVENVGLLGLTRVGSRRVVQISTAFM
Sbjct: 340 VSRSIGLQGIGQLFDGFFGAIVGTTASVENVGLLGLTRVGSRRVVQISTAFMIFFSIFGK 399
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
AQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLG+SLFLGISIPQYF
Sbjct: 400 FGAFFAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGVSLFLGISIPQYF 459
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNTDIAGHGPV TP+GWFNDILNTIFSSPPTVATIVGT +DNTLEAR S DDRG+PWLV
Sbjct: 460 VMNTDIAGHGPVRTPAGWFNDILNTIFSSPPTVATIVGTVVDNTLEARHSYDDRGIPWLV 519
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQRRKGDSRN+EFYSYPLRINEYIPSRFL
Sbjct: 520 PFQRRKGDSRNEEFYSYPLRINEYIPSRFL 549
>ref|XP_006473388.1| PREDICTED: nucleobase-ascorbate transporter 3-like isoform X1 [Citrus
sinensis] gi|568838793|ref|XP_006473389.1| PREDICTED:
nucleobase-ascorbate transporter 3-like isoform X2
[Citrus sinensis] gi|568838795|ref|XP_006473390.1|
PREDICTED: nucleobase-ascorbate transporter 3-like
isoform X3 [Citrus sinensis]
gi|568838797|ref|XP_006473391.1| PREDICTED:
nucleobase-ascorbate transporter 3-like isoform X4
[Citrus sinensis]
Length = 543
Score = 393 bits (1009), Expect = e-107
Identities = 200/270 (74%), Positives = 217/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LLSSAPWIKVPYPFQWGTPIFRAS VFGM+GAALV+SAEST TF AASR AGAT PPAHV
Sbjct: 274 LLSSAPWIKVPYPFQWGTPIFRASHVFGMIGAALVTSAESTGTFIAASRFAGATAPPAHV 333
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G+VVG TASVENVGLLGLT +GSRRVVQISTAFM
Sbjct: 334 LSRSIGLQGIGMLVEGIFGSVVGTTASVENVGLLGLTHIGSRRVVQISTAFMIFFSIFGK 393
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L GIVAA+GI+FIQFAN NSMRNIY+LG+SLFLGISIPQYF
Sbjct: 394 FGAFFASIPLPIFAAIYCVLLGIVAAVGITFIQFANNNSMRNIYILGLSLFLGISIPQYF 453
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
T GHGPV T GWFNDI NTIFSSPPTVA IVGT +DNTL+AR ++ DRG+PW
Sbjct: 454 ASKTTEDGHGPVRTGGGWFNDIWNTIFSSPPTVAMIVGTVLDNTLDARDTVADRGLPWWK 513
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGDSRNDEFYS+PLRINEYIP+RFL
Sbjct: 514 PFQHRKGDSRNDEFYSFPLRINEYIPTRFL 543
>ref|XP_006434867.1| hypothetical protein CICLE_v10000779mg [Citrus clementina]
gi|567884619|ref|XP_006434868.1| hypothetical protein
CICLE_v10000779mg [Citrus clementina]
gi|567884621|ref|XP_006434869.1| hypothetical protein
CICLE_v10000779mg [Citrus clementina]
gi|557536989|gb|ESR48107.1| hypothetical protein
CICLE_v10000779mg [Citrus clementina]
gi|557536990|gb|ESR48108.1| hypothetical protein
CICLE_v10000779mg [Citrus clementina]
gi|557536991|gb|ESR48109.1| hypothetical protein
CICLE_v10000779mg [Citrus clementina]
Length = 543
Score = 392 bits (1006), Expect = e-106
Identities = 199/270 (73%), Positives = 217/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LLSSAPWIKVPYPFQWGTPIFRAS VFGM+GAALV+SAEST TF AASR AGAT PPAHV
Sbjct: 274 LLSSAPWIKVPYPFQWGTPIFRASHVFGMIGAALVTSAESTGTFIAASRFAGATAPPAHV 333
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G++VG TASVENVGLLGLT +GSRRVVQISTAFM
Sbjct: 334 LSRSIGLQGIGMLVEGIFGSLVGTTASVENVGLLGLTHIGSRRVVQISTAFMIFFSIFGK 393
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L GIVAA+GI+FIQFAN NSMRNIY+LG+SLFLGISIPQYF
Sbjct: 394 FGAFFASIPLPIFAAIYCVLLGIVAAVGITFIQFANNNSMRNIYILGLSLFLGISIPQYF 453
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
T GHGPV T GWFNDI NTIFSSPPTVA IVGT +DNTL+AR ++ DRG+PW
Sbjct: 454 ASKTTEDGHGPVRTGGGWFNDIWNTIFSSPPTVAMIVGTVLDNTLDARDTVADRGLPWWK 513
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGDSRNDEFYS+PLRINEYIP+RFL
Sbjct: 514 PFQHRKGDSRNDEFYSFPLRINEYIPTRFL 543
>gb|EXB38630.1| Nucleobase-ascorbate transporter 3 [Morus notabilis]
Length = 489
Score = 389 bits (1000), Expect = e-106
Identities = 196/270 (72%), Positives = 219/270 (81%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWI++PYPFQWGTPIF+AS VFGMMGAALV+SAEST TF+AA+RL+GAT PPA V
Sbjct: 220 LISSAPWIRIPYPFQWGTPIFKASHVFGMMGAALVASAESTGTFFAAARLSGATAPPAFV 279
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI GA G+TASVENVGLLGLT +GSRRVVQISTAFM
Sbjct: 280 LSRSIGMQGIGMLLEGIFGAAFGNTASVENVGLLGLTHIGSRRVVQISTAFMFFFSIFGK 339
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA GISFIQFAN NSMRN+Y+LG+SLFLGISIPQYF
Sbjct: 340 FGAFFASIPLPIFAAIYCVLFGIVAASGISFIQFANSNSMRNLYILGLSLFLGISIPQYF 399
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNT G GPV T +GWF+DILNTIFSSPPTVA IVGT +DNTLE++ +IDDRGVPW
Sbjct: 400 VMNTTADGRGPVRTDAGWFDDILNTIFSSPPTVAMIVGTLLDNTLESKYAIDDRGVPWWR 459
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGD RNDEFY PLRI+EYIPSRFL
Sbjct: 460 PFQHRKGDPRNDEFYGLPLRIHEYIPSRFL 489
>ref|XP_004499100.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Cicer arietinum]
Length = 548
Score = 387 bits (995), Expect = e-105
Identities = 193/270 (71%), Positives = 217/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LLSSAPWIK PYPFQWGTPIF+AS VFGMMGAALVSSAEST TF+AA+RL+GATPPPAHV
Sbjct: 279 LLSSAPWIKFPYPFQWGTPIFKASHVFGMMGAALVSSAESTGTFFAAARLSGATPPPAHV 338
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
++RSI G++VG TASVENVGLLGLT +GSRRVVQIS AFM
Sbjct: 339 LTRSIGLQGLSMLLEGIFGSIVGTTASVENVGLLGLTHIGSRRVVQISCAFMIFFSIFGK 398
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA+GISFIQF N NS+RNIYV G++LFLGISIPQYF
Sbjct: 399 FGAFFASIPLPIFAAIYCVLFGIVAAVGISFIQFTNNNSIRNIYVFGLTLFLGISIPQYF 458
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
+MNT GHGPV T GWFNDILNTIFSSPPTVA IVGT +DNTLEA+Q+ DRG+ W
Sbjct: 459 IMNTSPDGHGPVRTNGGWFNDILNTIFSSPPTVAIIVGTVLDNTLEAKQTAVDRGMQWWA 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ +KGD RNDEFY +PLR+ EYIP+RFL
Sbjct: 519 PFQNKKGDVRNDEFYRFPLRLTEYIPTRFL 548
>ref|XP_006386511.1| PIGMENT DEFECTIVE EMBRYO 135 family protein [Populus trichocarpa]
gi|566157753|ref|XP_006386512.1| hypothetical protein
POPTR_0002s13080g [Populus trichocarpa]
gi|550344899|gb|ERP64308.1| PIGMENT DEFECTIVE EMBRYO 135
family protein [Populus trichocarpa]
gi|550344900|gb|ERP64309.1| hypothetical protein
POPTR_0002s13080g [Populus trichocarpa]
Length = 550
Score = 386 bits (992), Expect = e-105
Identities = 197/270 (72%), Positives = 218/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSA W+KVPYPFQWG PIFRAS VFGM+GAALVSSAEST TF+AA+RLAGAT PPAHV
Sbjct: 281 LMSSASWVKVPYPFQWGAPIFRASHVFGMIGAALVSSAESTGTFFAAARLAGATHPPAHV 340
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI GA VG TASVENVGLLGLT VGSRRVVQISTAFM
Sbjct: 341 LSRSIGLQGVSLLLDGIFGAAVGTTASVENVGLLGLTHVGSRRVVQISTAFMFFFSIFGK 400
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAAIGISFIQF+N NSMRN Y+LG++LFLGISIPQYF
Sbjct: 401 FGALFASIPLPIFAAIYCVLFGIVAAIGISFIQFSNNNSMRNHYILGMALFLGISIPQYF 460
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
V NT GHGPV T GWFNDILNT+FSSPPTVA IVGT +D+TLEARQ+I+DRG+PW
Sbjct: 461 VSNTTGDGHGPVRTNGGWFNDILNTLFSSPPTVAMIVGTLLDSTLEARQTINDRGIPWWK 520
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGD R DEFYS PLRINE++P+RFL
Sbjct: 521 PFQSRKGDVRTDEFYSLPLRINEWMPTRFL 550
>ref|XP_003589253.1| Nucleobase-ascorbate transporter [Medicago truncatula]
gi|355478301|gb|AES59504.1| Nucleobase-ascorbate
transporter [Medicago truncatula]
Length = 549
Score = 386 bits (992), Expect = e-105
Identities = 195/270 (72%), Positives = 217/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LL+ APWI VPYPFQWGTPIF+AS VFGMMGAALV+S EST TF+AA+RL+GATPPPAHV
Sbjct: 280 LLTRAPWIYVPYPFQWGTPIFKASHVFGMMGAALVTSFESTGTFFAAARLSGATPPPAHV 339
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G+VVG TASVENVGLLGLT +GSRRVVQ+S FM
Sbjct: 340 LSRSIGLQGISMLIEGICGSVVGTTASVENVGLLGLTHIGSRRVVQMSCGFMILCSIFGK 399
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA GISFIQFAN NS+RNIYV G++LFLGISIPQYF
Sbjct: 400 FGAFFASIPLPIFAAIYCVLFGIVAATGISFIQFANNNSIRNIYVFGLTLFLGISIPQYF 459
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNT GHGPV T GWFNDILNTIFSSPPTVA IVGT +DNTLEA+Q+ DRG+PW V
Sbjct: 460 VMNTAPDGHGPVRTNGGWFNDILNTIFSSPPTVAIIVGTVLDNTLEAKQTAVDRGLPWWV 519
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ+RKGD RNDEFY +PLR+ EYIPSRFL
Sbjct: 520 PFQKRKGDVRNDEFYRFPLRLTEYIPSRFL 549
>ref|XP_003544440.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Glycine max]
Length = 548
Score = 384 bits (986), Expect = e-104
Identities = 195/270 (72%), Positives = 214/270 (79%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWIKVPYPFQWGTPIFRAS VFGMMGAALVSSAEST F+AA+RL+GATPPPAHV
Sbjct: 279 LMSSAPWIKVPYPFQWGTPIFRASHVFGMMGAALVSSAESTGAFFAAARLSGATPPPAHV 338
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G++VG T SVENVGLLGLT +GSRRVVQIS FM
Sbjct: 339 LSRSIGMQGIGMLLEGIFGSIVGTTVSVENVGLLGLTHIGSRRVVQISCGFMIFFSIFGK 398
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA GISFIQFAN NS+RNIYVLG++LFL ISIPQYF
Sbjct: 399 FGAFFASIPLPIFAAIYCVLFGIVAATGISFIQFANTNSIRNIYVLGLTLFLAISIPQYF 458
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNT GHGPV T GWFNDILNTIFSS PTVA IVGT +DNTLE +Q+ DRG+PW
Sbjct: 459 VMNTAPDGHGPVRTGGGWFNDILNTIFSSAPTVAIIVGTLVDNTLEGKQTAVDRGLPWWG 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGD RNDEFY PLRINEY+P+RFL
Sbjct: 519 PFQNRKGDVRNDEFYRLPLRINEYMPTRFL 548
>ref|XP_002282229.1| PREDICTED: nucleobase-ascorbate transporter 3 [Vitis vinifera]
gi|302142380|emb|CBI19583.3| unnamed protein product
[Vitis vinifera]
Length = 545
Score = 384 bits (985), Expect = e-104
Identities = 191/270 (70%), Positives = 217/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SS+PWI++PYPFQWG P+FRAS VFGMMGAALV+SAEST TF+AA+RLAGATPPP HV
Sbjct: 276 LISSSPWIRIPYPFQWGPPVFRASHVFGMMGAALVTSAESTGTFFAAARLAGATPPPPHV 335
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI GAVVG TASVENVGLLGLT +GSRRVVQISTAFM
Sbjct: 336 LSRSIGLQGISLLLDGLFGAVVGTTASVENVGLLGLTHIGSRRVVQISTAFMFFFSIFGK 395
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA GISF+QFAN NSMRN+YVLG+SLFLG+SI QYF
Sbjct: 396 FGAFFASIPLPIFAAIYCVLFGIVAATGISFLQFANSNSMRNLYVLGLSLFLGVSISQYF 455
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
V +T GHGPV T GWFNDILNTIFSSPPTVA IVGT +DNTL+ R+ DDRG+ WLV
Sbjct: 456 VSHTTTDGHGPVKTDGGWFNDILNTIFSSPPTVAIIVGTLLDNTLDPRRFHDDRGIQWLV 515
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PF KGDSRN+EFY+ PLRINEY+P+R+L
Sbjct: 516 PFHHWKGDSRNEEFYNLPLRINEYMPTRYL 545
>gb|EOY14596.1| Xanthine/uracil permease family protein isoform 1 [Theobroma cacao]
Length = 548
Score = 383 bits (984), Expect = e-104
Identities = 193/270 (71%), Positives = 218/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWIK+PYPFQWGTPIFRA+ VFGM+GAALVSSAEST TF+AA+RL+GAT PPAHV
Sbjct: 280 LMSSAPWIKIPYPFQWGTPIFRATHVFGMIGAALVSSAESTGTFFAAARLSGATAPPAHV 339
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G++VG TASVENVGLLGLT +GSRRVVQIST FM
Sbjct: 340 LSRSIGLQGVGMLIEGLFGSLVGTTASVENVGLLGLTHIGSRRVVQISTGFMIFFSIFGK 399
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L GIVAA GI+FIQFAN NSMRNIYVLG+SLFLG+SIPQYF
Sbjct: 400 FGAFFASIPLPIFAAIYCVLLGIVAATGITFIQFANNNSMRNIYVLGVSLFLGLSIPQYF 459
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
MNT + GHGPV T +GWFN+ILNTIFSSP TVA IVGT +DNTLEA+ +DDRGVPW
Sbjct: 460 AMNTPLDGHGPVRTDAGWFNNILNTIFSSPATVAIIVGTVLDNTLEAKH-VDDRGVPWWK 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ KGD R +EFYSYPLR+NEY+P+RFL
Sbjct: 519 PFQHSKGDVRTEEFYSYPLRLNEYMPTRFL 548
>gb|AFK46481.1| unknown [Medicago truncatula]
Length = 549
Score = 383 bits (984), Expect = e-104
Identities = 194/270 (71%), Positives = 216/270 (80%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
LL+ APWI VPYPFQWGTPIF+AS VFGMMGAALV+S EST TF+AA+RL+GATPPPA V
Sbjct: 280 LLTRAPWIYVPYPFQWGTPIFKASHVFGMMGAALVTSFESTGTFFAAARLSGATPPPARV 339
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G+VVG TASVENVGLLGLT +GSRRVVQ+S FM
Sbjct: 340 LSRSIGLQGISMLIEGICGSVVGTTASVENVGLLGLTHIGSRRVVQMSCGFMTLCSIFGK 399
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA GISFIQFAN NS+RNIYV G++LFLGISIPQYF
Sbjct: 400 FGAFFASIPLPIFAAIYCVLFGIVAATGISFIQFANNNSIRNIYVFGLTLFLGISIPQYF 459
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNT GHGPV T GWFNDILNTIFSSPPTVA IVGT +DNTLEA+Q+ DRG+PW V
Sbjct: 460 VMNTAPDGHGPVRTNGGWFNDILNTIFSSPPTVAIIVGTVLDNTLEAKQTAVDRGLPWWV 519
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ+RKGD RNDEFY +PLR+ EYIPSRFL
Sbjct: 520 PFQKRKGDVRNDEFYRFPLRLTEYIPSRFL 549
>ref|XP_003550352.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Glycine max]
Length = 548
Score = 383 bits (984), Expect = e-104
Identities = 196/270 (72%), Positives = 214/270 (79%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWIKVPYPFQWGTPIFRAS VFGMMGAALVSSAEST F+AA+RL+GATPPPAHV
Sbjct: 279 LMSSAPWIKVPYPFQWGTPIFRASHVFGMMGAALVSSAESTGGFFAAARLSGATPPPAHV 338
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G+VVG T SVENVGLLGLT +GSRRVVQIS +M
Sbjct: 339 LSRSIGMQGIGMLLEGIFGSVVGTTVSVENVGLLGLTHIGSRRVVQISCGYMIFFSIFGK 398
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA GISFIQFAN NS+RNIYVLG++LFL ISIPQYF
Sbjct: 399 FGAFFASIPLPIFAAIYCVLFGIVAATGISFIQFANTNSIRNIYVLGLTLFLAISIPQYF 458
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
VMNT GHGPV T GWFNDILNTIFSS PTVA IVGT IDNTLE +Q+ DRG+PW
Sbjct: 459 VMNTAPDGHGPVRTDGGWFNDILNTIFSSAPTVAIIVGTLIDNTLEGKQTAVDRGLPWWG 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGD RNDEFY PLRINEY+P+RFL
Sbjct: 519 PFQNRKGDVRNDEFYRLPLRINEYMPTRFL 548
>gb|EOY14597.1| Xanthine/uracil permease family protein isoform 2 [Theobroma cacao]
Length = 549
Score = 379 bits (972), Expect = e-102
Identities = 192/271 (70%), Positives = 217/271 (80%), Gaps = 1/271 (0%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWIK+PYPFQWGTPIFRA+ VFGM+GAALVSSAEST TF+AA+RL+GAT PPAHV
Sbjct: 280 LMSSAPWIKIPYPFQWGTPIFRATHVFGMIGAALVSSAESTGTFFAAARLSGATAPPAHV 339
Query: 795 VSRSIXXXXXXXXXXXXXG-AVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXX 619
+SRSI ++VG TASVENVGLLGLT +GSRRVVQIST FM
Sbjct: 340 LSRSIGLQQGVGMLIEGLFGSLVGTTASVENVGLLGLTHIGSRRVVQISTGFMIFFSIFG 399
Query: 618 XXXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQY 439
A IPLPIFAAIYC+L GIVAA GI+FIQFAN NSMRNIYVLG+SLFLG+SIPQY
Sbjct: 400 KFGAFFASIPLPIFAAIYCVLLGIVAATGITFIQFANNNSMRNIYVLGVSLFLGLSIPQY 459
Query: 438 FVMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWL 259
F MNT + GHGPV T +GWFN+ILNTIFSSP TVA IVGT +DNTLEA+ +DDRGVPW
Sbjct: 460 FAMNTPLDGHGPVRTDAGWFNNILNTIFSSPATVAIIVGTVLDNTLEAKH-VDDRGVPWW 518
Query: 258 VPFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ KGD R +EFYSYPLR+NEY+P+RFL
Sbjct: 519 KPFQHSKGDVRTEEFYSYPLRLNEYMPTRFL 549
>gb|ESW32808.1| hypothetical protein PHAVU_001G018700g [Phaseolus vulgaris]
Length = 548
Score = 377 bits (967), Expect = e-102
Identities = 194/270 (71%), Positives = 210/270 (77%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWIKVPYPFQWGTPIFRAS VFGMMGAALVSSAEST F+AA+RL+GATPPPAHV
Sbjct: 279 LMSSAPWIKVPYPFQWGTPIFRASHVFGMMGAALVSSAESTGGFFAAARLSGATPPPAHV 338
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI G+VVG T SVENVGLLGLT +GSRRVVQIS FM
Sbjct: 339 LSRSIGMQGIGMLLEGIFGSVVGTTVSVENVGLLGLTHIGSRRVVQISCGFMIFFSIFGK 398
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA+GISFIQFAN NS+RNIYVLG+SLFL ISIPQYF
Sbjct: 399 FGAFFASIPLPIFAAIYCVLFGIVAAVGISFIQFANTNSIRNIYVLGLSLFLAISIPQYF 458
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
V NT GHGPV T GWFNDILNTIFSS PTVA IVG IDNTLE + DRG+ W
Sbjct: 459 VTNTAPDGHGPVKTDGGWFNDILNTIFSSSPTVAIIVGILIDNTLEGKLKAVDRGLQWWS 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGD RNDEFY PLRINEY+P+RFL
Sbjct: 519 PFQNRKGDVRNDEFYRLPLRINEYMPTRFL 548
>ref|XP_002510280.1| purine permease, putative [Ricinus communis]
gi|223550981|gb|EEF52467.1| purine permease, putative
[Ricinus communis]
Length = 548
Score = 376 bits (966), Expect = e-102
Identities = 188/270 (69%), Positives = 214/270 (79%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPW++VPYPFQWG PIFRAS VFGMMGAALVSSAEST ++AA+RL+GAT PPAHV
Sbjct: 279 LISSAPWVRVPYPFQWGAPIFRASHVFGMMGAALVSSAESTGAYFAAARLSGATHPPAHV 338
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
++RSI GA VG+T SVENVGLLG+T +GSRRVVQISTAFM
Sbjct: 339 LTRSIGLQGVGMLLEGIFGAAVGNTVSVENVGLLGMTNIGSRRVVQISTAFMIFFSIFGK 398
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPL IFAAIYC+L+GIVAAIGISFIQF+N NSMRN Y+LG+SLFLGISIPQYF
Sbjct: 399 FGAFFASIPLTIFAAIYCVLFGIVAAIGISFIQFSNNNSMRNHYILGLSLFLGISIPQYF 458
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
NT I GHGPV T GWFNDILNTIFSSPPTVA VGT +D+TL+AR + +DRG+PW
Sbjct: 459 ASNTTIDGHGPVRTDGGWFNDILNTIFSSPPTVAMTVGTVLDSTLDARHTTNDRGLPWWK 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGD R +EFYS PLRINE++PSRFL
Sbjct: 519 PFQHRKGDVRTEEFYSLPLRINEWLPSRFL 548
>ref|XP_006375005.1| PIGMENT DEFECTIVE EMBRYO 135 family protein [Populus trichocarpa]
gi|550323319|gb|ERP52802.1| PIGMENT DEFECTIVE EMBRYO 135
family protein [Populus trichocarpa]
Length = 547
Score = 374 bits (960), Expect = e-101
Identities = 187/270 (69%), Positives = 214/270 (79%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SS+PW++VPYPFQWG P FRAS VFGM+GAALVSSAEST TF+AASRLAGAT PPAHV
Sbjct: 278 LVSSSPWVRVPYPFQWGAPKFRASHVFGMIGAALVSSAESTGTFFAASRLAGATSPPAHV 337
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+SRSI GA VG TASVENVGLLGLT +GSRRVVQ+STAFM
Sbjct: 338 LSRSIGLQGVGLLLNGIFGAAVGTTASVENVGLLGLTHIGSRRVVQVSTAFMFFFSIFGK 397
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPL IFAA YC+LYGIVAAIGISFIQF+N NSMRN YVLG++LFLGISIPQYF
Sbjct: 398 FGALFASIPLAIFAAAYCVLYGIVAAIGISFIQFSNNNSMRNHYVLGLALFLGISIPQYF 457
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
V NT GHGPV GWFNDILNT+FSSPPTVA IVGT +DN LEA+++ DRG+PW
Sbjct: 458 VSNTTGDGHGPVRADGGWFNDILNTVFSSPPTVAMIVGTLLDNLLEAKKNATDRGIPWWK 517
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGDSR+DE+YS P+R+NE++P+R+L
Sbjct: 518 PFQNRKGDSRSDEYYSMPIRMNEWMPTRYL 547
>gb|EMJ26942.1| hypothetical protein PRUPE_ppa003778mg [Prunus persica]
Length = 549
Score = 372 bits (956), Expect = e-101
Identities = 187/270 (69%), Positives = 215/270 (79%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPW+++PYPFQWG PIFRAS VFGMMGAALV+SAEST T++AA+RLAGATPPPA V
Sbjct: 280 LISSAPWVRIPYPFQWGKPIFRASHVFGMMGAALVTSAESTGTYFAAARLAGATPPPASV 339
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+S+SI GA VG TASVENVGLLGLT +GSRRVVQISTAFM
Sbjct: 340 ISQSIGLQGVGMLLEGIFGAAVGTTASVENVGLLGLTHIGSRRVVQISTAFMFFFSIFGK 399
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIFAAIYC+L+GIVAA+GI+FIQF N NS+RNIYVLG+SLFLGISIPQYF
Sbjct: 400 FGAFFASIPLPIFAAIYCVLFGIVAAVGITFIQFTNNNSLRNIYVLGLSLFLGISIPQYF 459
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
+ NT G GPV T WF+DI+NTIFSS PTVA IVGT +DNTL+A+ S+DDRG+ W
Sbjct: 460 ISNTTPNGVGPVRTDGIWFDDIVNTIFSSSPTVAIIVGTLLDNTLDAKYSVDDRGLAWWR 519
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRFL 166
PFQ RKGDSRN+EFYS P+RI EYIPSR+L
Sbjct: 520 PFQSRKGDSRNEEFYSLPVRIREYIPSRYL 549
>ref|XP_004160603.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Cucumis sativus]
Length = 544
Score = 372 bits (955), Expect = e-100
Identities = 182/269 (67%), Positives = 212/269 (78%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWI+VPYPFQWGTPIFRAS VFGMMGA LV+SAEST TF+AA+RL+GATPPPA++
Sbjct: 275 LMSSAPWIRVPYPFQWGTPIFRASHVFGMMGATLVASAESTGTFFAAARLSGATPPPAYI 334
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+RSI G++ G++ASVENVGLLGLT +GSRRVVQIST FM
Sbjct: 335 FNRSIGLQGIGLLVEGIFGSIAGNSASVENVGLLGLTHIGSRRVVQISTGFMIFFSIFGK 394
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIF AIYC+L+GIVAA GISF+QF N NSMRN+Y++G+SLFLGISIPQYF
Sbjct: 395 FGAFFASIPLPIFGAIYCVLFGIVAATGISFMQFTNNNSMRNLYIIGLSLFLGISIPQYF 454
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
V NT G GPV T GWFNDILNTIFSS PT+A I+GT +D TL+A+ SI+DRGV W
Sbjct: 455 VTNTSQDGRGPVQTAGGWFNDILNTIFSSAPTIAIIIGTVLDQTLDAKHSINDRGVSWWK 514
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRF 169
PFQ +KGD+RNDEFY PLRINEYIP+RF
Sbjct: 515 PFQHKKGDTRNDEFYGLPLRINEYIPTRF 543
>ref|XP_004141239.1| PREDICTED: nucleobase-ascorbate transporter 3-like [Cucumis sativus]
Length = 548
Score = 372 bits (955), Expect = e-100
Identities = 182/269 (67%), Positives = 212/269 (78%)
Frame = -1
Query: 975 LLSSAPWIKVPYPFQWGTPIFRASSVFGMMGAALVSSAESTATFYAASRLAGATPPPAHV 796
L+SSAPWI+VPYPFQWGTPIFRAS VFGMMGA LV+SAEST TF+AA+RL+GATPPPA++
Sbjct: 279 LMSSAPWIRVPYPFQWGTPIFRASHVFGMMGATLVASAESTGTFFAAARLSGATPPPAYI 338
Query: 795 VSRSIXXXXXXXXXXXXXGAVVGHTASVENVGLLGLTRVGSRRVVQISTAFMXXXXXXXX 616
+RSI G++ G++ASVENVGLLGLT +GSRRVVQIST FM
Sbjct: 339 FNRSIGLQGIGLLVEGIFGSIAGNSASVENVGLLGLTHIGSRRVVQISTGFMIFFSIFGK 398
Query: 615 XXXXXAQIPLPIFAAIYCILYGIVAAIGISFIQFANKNSMRNIYVLGISLFLGISIPQYF 436
A IPLPIF AIYC+L+GIVAA GISF+QF N NSMRN+Y++G+SLFLGISIPQYF
Sbjct: 399 FGAFFASIPLPIFGAIYCVLFGIVAATGISFMQFTNNNSMRNLYIIGLSLFLGISIPQYF 458
Query: 435 VMNTDIAGHGPVGTPSGWFNDILNTIFSSPPTVATIVGTFIDNTLEARQSIDDRGVPWLV 256
V NT G GPV T GWFNDILNTIFSS PT+A I+GT +D TL+A+ SI+DRGV W
Sbjct: 459 VTNTSQDGRGPVQTAGGWFNDILNTIFSSAPTIAIIIGTVLDQTLDAKHSINDRGVSWWK 518
Query: 255 PFQRRKGDSRNDEFYSYPLRINEYIPSRF 169
PFQ +KGD+RNDEFY PLRINEYIP+RF
Sbjct: 519 PFQHKKGDTRNDEFYGLPLRINEYIPTRF 547