BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00030235
         (1106 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]             275   e-119
emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|29835728...   251   e-102
gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana]         249   e-100
gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]         249   4e-99
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              215   2e-77

>dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]
          Length = 788

 Score =  275 bits (704), Expect(2) = e-119
 Identities = 133/158 (84%), Positives = 146/158 (92%)
 Frame = +3

Query: 633  SPLLKDTLSSTLACILALKRWNVREDQINKGLCFIESNFASAIDKNQPSPFGFDIIFLGM 812
            SPL+KDTLSSTLAC+LALKRWNV +DQINKGL +IESNFAS  DKNQ SPFGFDIIF GM
Sbjct: 118  SPLIKDTLSSTLACVLALKRWNVGKDQINKGLHYIESNFASVTDKNQASPFGFDIIFPGM 177

Query: 813  LDYAKDLNIRLPLNQTDLTMMLHERELELRRCHSNEREAYLAYISEGLGNFQDWDMVMKY 992
            L+YAKDL+I+LPLNQT L++MLHERELELRRCHSN REAYLAYISEGLGN  DW+MVMKY
Sbjct: 178  LEYAKDLDIKLPLNQTHLSVMLHERELELRRCHSNGREAYLAYISEGLGNLNDWNMVMKY 237

Query: 993  QMKNGSLFNSPSATAAALIHHQNAGCLNYLSSLLQKFG 1106
            QMKNGSLFNSPSATA+ LIHHQNAGCL+YL+SLL KFG
Sbjct: 238  QMKNGSLFNSPSATASVLIHHQNAGCLHYLTSLLDKFG 275



 Score =  180 bits (456), Expect(2) = e-119
 Identities = 82/120 (68%), Positives = 96/120 (80%)
 Frame = +1

Query: 289 IAHSTSSVIVVPSLHRAHQPWLVGCCMQRYNRSALHTVSSHAVQANPPILTLDTAKERIR 468
           IA  TSS ++VPS H  H+ W+V CCM +YN S L T SS A Q NP ++TLD  KERIR
Sbjct: 3   IAQITSSAMLVPSSHIPHRSWVVNCCMVQYNPSGLRTASSQAGQVNPTVMTLDVTKERIR 62

Query: 469 KLFSNVELSVSSYDTAWVAMVPSPNSPKSPCFPECLNWVMDNQLNDGSWGLLNHHPHYLK 648
           KLF+NVE+SVSSYDTAWVAMVPSPNSPKSPCFP+CLNW++DNQL+DGSWGLL H    +K
Sbjct: 63  KLFNNVEVSVSSYDTAWVAMVPSPNSPKSPCFPDCLNWLLDNQLDDGSWGLLPHQSPLIK 122


>emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|298357288|emb|CBM82409.1|
            kaurene synthase [Helianthus annuus]
          Length = 772

 Score =  251 bits (640), Expect(2) = e-102
 Identities = 122/157 (77%), Positives = 138/157 (87%)
 Frame = +3

Query: 636  PLLKDTLSSTLACILALKRWNVREDQINKGLCFIESNFASAIDKNQPSPFGFDIIFLGML 815
            PLLKD+LSSTLACI+ALKRWNV E QINKGL FIESNFASA DK+ PSPFGFDIIF GM+
Sbjct: 108  PLLKDSLSSTLACIVALKRWNVGEAQINKGLHFIESNFASATDKSHPSPFGFDIIFPGMV 167

Query: 816  DYAKDLNIRLPLNQTDLTMMLHERELELRRCHSNEREAYLAYISEGLGNFQDWDMVMKYQ 995
            +YA+ LNI L  NQTD T+MLHERELEL+RCHS+E +AYLAYISEGLGN  DW +V KYQ
Sbjct: 168  EYAERLNINLRSNQTDFTLMLHERELELKRCHSSEMKAYLAYISEGLGNLYDWHIVKKYQ 227

Query: 996  MKNGSLFNSPSATAAALIHHQNAGCLNYLSSLLQKFG 1106
            +KNGS+FNSPSATAAA I+HQ+ GCLNYL+SLL KFG
Sbjct: 228  LKNGSVFNSPSATAAAFINHQDTGCLNYLTSLLDKFG 264



 Score =  148 bits (373), Expect(2) = e-102
 Identities = 68/87 (78%), Positives = 77/87 (88%), Gaps = 3/87 (3%)
 Frame = +1

Query: 385 SALHTVSSHAVQANPPILTLDTAKERIRKLFSNVELSVSSYDTAWVAMVPSPNSPKSPCF 564
           SA+HT SSH  QANP  L +DT KERIRKLF+NV+LSVSSYDTAWVAMVPSP+SPKSPCF
Sbjct: 22  SAIHTASSHVGQANPSNLAIDTTKERIRKLFNNVDLSVSSYDTAWVAMVPSPSSPKSPCF 81

Query: 565 PECLNWVMDNQLNDGSWGLLNH---HP 636
           PECLNW++DNQL+DGSWGL+NH   HP
Sbjct: 82  PECLNWLVDNQLDDGSWGLVNHSRTHP 108


>gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana]
          Length = 784

 Score =  249 bits (637), Expect(2) = e-100
 Identities = 121/157 (77%), Positives = 137/157 (87%)
 Frame = +3

Query: 636  PLLKDTLSSTLACILALKRWNVREDQINKGLCFIESNFASAIDKNQPSPFGFDIIFLGML 815
            PLLKD+LSSTLACI+ALKRWNV EDQINKGL FIESN ASA DK+QPSP GFDIIF G+L
Sbjct: 111  PLLKDSLSSTLACIVALKRWNVGEDQINKGLSFIESNLASATDKSQPSPIGFDIIFPGLL 170

Query: 816  DYAKDLNIRLPLNQTDLTMMLHERELELRRCHSNEREAYLAYISEGLGNFQDWDMVMKYQ 995
            +YAK+L+I L   QTD ++MLH+RELE +RCHSNE + YLAYISEGLGN  DW+MV KYQ
Sbjct: 171  EYAKNLDINLLSKQTDFSLMLHKRELEQKRCHSNEIDGYLAYISEGLGNLYDWNMVKKYQ 230

Query: 996  MKNGSLFNSPSATAAALIHHQNAGCLNYLSSLLQKFG 1106
            MKNGS+FNSPSATAAA I+HQN GCLNYL+SLL KFG
Sbjct: 231  MKNGSVFNSPSATAAAFINHQNPGCLNYLNSLLDKFG 267



 Score =  142 bits (358), Expect(2) = e-100
 Identities = 67/87 (77%), Positives = 75/87 (86%), Gaps = 2/87 (2%)
 Frame = +1

Query: 385 SALHTVS-SHAVQANPPILTLDTAKERIRKLFSNVELSVSSYDTAWVAMVPSPNSPKSPC 561
           SA+HT S SH  Q NP  L +DT KERI+KLF NVE+SVSSYDTAWVAMVPSPNSPKSPC
Sbjct: 22  SAIHTASTSHGGQTNPTNLIIDTTKERIQKLFKNVEISVSSYDTAWVAMVPSPNSPKSPC 81

Query: 562 FPECLNWVMDNQLNDGSWGLLNH-HPH 639
           FPECLNW+++NQLNDGSWGL+NH H H
Sbjct: 82  FPECLNWLINNQLNDGSWGLVNHTHNH 108


>gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]
          Length = 784

 Score =  249 bits (635), Expect(2) = 4e-99
 Identities = 120/157 (76%), Positives = 137/157 (87%)
 Frame = +3

Query: 636  PLLKDTLSSTLACILALKRWNVREDQINKGLCFIESNFASAIDKNQPSPFGFDIIFLGML 815
            PLLKD+LSSTLACI+ALKRWNV EDQINKGL FIESN ASA +K+QPSP GFDIIF G+L
Sbjct: 111  PLLKDSLSSTLACIVALKRWNVGEDQINKGLSFIESNLASATEKSQPSPIGFDIIFPGLL 170

Query: 816  DYAKDLNIRLPLNQTDLTMMLHERELELRRCHSNEREAYLAYISEGLGNFQDWDMVMKYQ 995
            +YAK+L+I L   QTD ++MLH+RELE +RCHSNE + YLAYISEGLGN  DW+MV KYQ
Sbjct: 171  EYAKNLDINLLSKQTDFSLMLHKRELEQKRCHSNEMDGYLAYISEGLGNLYDWNMVKKYQ 230

Query: 996  MKNGSLFNSPSATAAALIHHQNAGCLNYLSSLLQKFG 1106
            MKNGS+FNSPSATAAA I+HQN GCLNYL+SLL KFG
Sbjct: 231  MKNGSVFNSPSATAAAFINHQNPGCLNYLNSLLDKFG 267



 Score =  140 bits (352), Expect(2) = 4e-99
 Identities = 66/87 (75%), Positives = 74/87 (85%), Gaps = 2/87 (2%)
 Frame = +1

Query: 385 SALHTVS-SHAVQANPPILTLDTAKERIRKLFSNVELSVSSYDTAWVAMVPSPNSPKSPC 561
           SA+HT S SH  Q NP  L +DT KERI+K F NVE+SVSSYDTAWVAMVPSPNSPKSPC
Sbjct: 22  SAIHTASTSHGGQTNPTNLIIDTTKERIQKQFKNVEISVSSYDTAWVAMVPSPNSPKSPC 81

Query: 562 FPECLNWVMDNQLNDGSWGLLNH-HPH 639
           FPECLNW+++NQLNDGSWGL+NH H H
Sbjct: 82  FPECLNWLINNQLNDGSWGLVNHTHNH 108


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  215 bits (548), Expect(2) = 2e-77
 Identities = 111/163 (68%), Positives = 129/163 (79%), Gaps = 4/163 (2%)
 Frame = +3

Query: 630  PSPLL-KDTLSSTLACILALKRWNVREDQINKGLCFIESNFASAIDKNQPSPFGFDIIFL 806
            P P+L KD LSSTLA +LALKRW V E+Q NKGL FI SNFAS  D+ Q SP GFDIIF 
Sbjct: 141  PHPMLVKDALSSTLASVLALKRWGVGEEQRNKGLWFIASNFASVSDEKQHSPIGFDIIFP 200

Query: 807  GMLDYAKDLNIRLPLNQTDLTMMLHERELELR---RCHSNEREAYLAYISEGLGNFQDWD 977
            GM++YAK+L++ LPL Q D+  ML +R+LEL+     ++  REAYLAYISEG+G  QDW+
Sbjct: 201  GMIEYAKELDLNLPLGQRDVDAMLQKRDLELKGSLGSNTKSREAYLAYISEGMGRLQDWE 260

Query: 978  MVMKYQMKNGSLFNSPSATAAALIHHQNAGCLNYLSSLLQKFG 1106
            MVMKYQMKNGSL NSPSATAAAL H QNAGCLNYL SLL+KFG
Sbjct: 261  MVMKYQMKNGSLLNSPSATAAALSHLQNAGCLNYLRSLLEKFG 303



 Score =  101 bits (252), Expect(2) = 2e-77
 Identities = 45/71 (63%), Positives = 57/71 (80%)
 Frame = +1

Query: 436 LTLDTAKERIRKLFSNVELSVSSYDTAWVAMVPSPNSPKSPCFPECLNWVMDNQLNDGSW 615
           L  +  KERI+K+F  VELSVSSYDTAWVAMVPSP S ++P FPEC+NW+++NQ +DGSW
Sbjct: 77  LGFEGTKERIKKMFDKVELSVSSYDTAWVAMVPSPYSSQAPYFPECVNWLLENQSHDGSW 136

Query: 616 GLLNHHPHYLK 648
           GL + HP  +K
Sbjct: 137 GLPHPHPMLVK 147