BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00005010
         (1990 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]             844   0.0  
gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana]         839   0.0  
gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum]   828   0.0  
gb|AFB77929.1| pectin methylesterase [Gossypium hirsutum]             819   0.0  
ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vini...   814   0.0  

>emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]
          Length = 579

 Score =  844 bits (2181), Expect = 0.0
 Identities = 424/579 (73%), Positives = 482/579 (83%), Gaps = 6/579 (1%)
 Frame = +3

Query: 93   DSGKNINLSNKRKKLFLTAFGSXXXXXXXXXXXXXXKSH-NSSSDNSTLQSITASAAHAA 269
            DSGKN+N S  +KKLFLT   S              K   N+S D++ +Q+IT SAAHA 
Sbjct: 3    DSGKNVNFSKGKKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAIT-SAAHAI 61

Query: 270  VKSSCSSTLYPDLCYSTITSHPELTKKVKTQKDVIELAVNITVTAVEHSYFQIKKLA-TR 446
            VKS+C +TL+P+LCYSTI S  + +KKV +QKDVIEL++NIT  AV+H++F+++KL  TR
Sbjct: 62   VKSACENTLHPELCYSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVEKLIKTR 121

Query: 447  KGLTKRQVGALHDCLEMVSETLDELHDVIKDLEEYKTKKTIRRHANDLKTLMSSAITNQE 626
            KGL  R+  ALHDCLE + ETLDELH  IKDLE Y  KK+++ HA+DLKTL+SSAITNQE
Sbjct: 122  KGLKPREKVALHDCLETIDETLDELHTAIKDLELYPNKKSLKAHADDLKTLISSAITNQE 181

Query: 627  TCLDGFSHDGADKKVRKVLIKVEDRVEKMCSNALAMICNMTNTDLANEMKLKG----RNL 794
            TCLDGFSHD ADKKVRK L+K +  VEKMCSNALAMICNMT+TD+ANE KLKG    R L
Sbjct: 182  TCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTDIANEQKLKGTTTNRKL 241

Query: 795  KEAEETVWPEWLSAGDRRLLQSGTVRPNVVVAADGSGNYRTXXXXXXXXPSKSSTRYVIR 974
            +E + + WPEWLSAGDRRLLQS TVRP+VVVAADGSGN++T        P KSS RYVIR
Sbjct: 242  RE-DNSEWPEWLSAGDRRLLQSSTVRPDVVVAADGSGNFKTVSEAVAKAPEKSSKRYVIR 300

Query: 975  IKAGVYRENVEVPKAKTKIMFMGDGRRNTIITGSRSVKGGSTTFESATVAIVGDGFLARD 1154
            IKAGVYRENV+VPK KT IMFMGDGR NTIITGSR+VK GSTTF SATVA VG+ FLARD
Sbjct: 301  IKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVGEKFLARD 360

Query: 1155 ITFQNTAGAANHQAVALRVGSDLSAFYQCDMLAYQDTLYVHRNRQFYINCLVVGTVDFIF 1334
            ITFQNTAGAA HQAVALRVGSDLSAFY+CD+LAYQD+LYVH NRQ+++ CL+ GTVDFIF
Sbjct: 361  ITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGTVDFIF 420

Query: 1335 GNAAAVFQDCDLHARRPDSGQKNMFTAQGRTDPNQNTGIVIQKCRIGATSDLLPVQGSFP 1514
            GNAAAV Q+CD+HARRP SGQKNM TAQGR+DPNQNTGIVIQKCRIGATSDL PVQ SFP
Sbjct: 421  GNAAAVLQNCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQKSFP 480

Query: 1515 TYLGRPWKEYSRTVVMQSSITNVINPAGWFEWNGNFALNTLYYGEYQNTGAGAGTSGRVK 1694
            TYLGRPWKEYSRTV+MQSSIT+VIN AGW EWNGNFALNTL+YGEYQNTGAGAGTSGRVK
Sbjct: 481  TYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGTSGRVK 540

Query: 1695 WKGFKVITSSTEAQGFTPGSFLGGGSWLRDTGFPFSLGL 1811
            WKGFKVITS+TEAQ +TPG F+ GGSWL  TGFPFSLGL
Sbjct: 541  WKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579


>gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana]
          Length = 579

 Score =  839 bits (2168), Expect = 0.0
 Identities = 422/579 (72%), Positives = 479/579 (82%), Gaps = 6/579 (1%)
 Frame = +3

Query: 93   DSGKNINLSNKRKKLFLTAFGSXXXXXXXXXXXXXXKSH-NSSSDNSTLQSITASAAHAA 269
            DSGKN+N S  +KKLFLT   S              K   N+S D++ +Q+IT SAAHA 
Sbjct: 3    DSGKNVNFSKGKKKLFLTVVASVLLVAAVIGVVVGVKFRSNNSDDHADIQAIT-SAAHAI 61

Query: 270  VKSSCSSTLYPDLCYSTITSHPELTKKVKTQKDVIELAVNITVTAVEHSYFQIKKLA-TR 446
            VKS+C +TL+P+LCYSTI S  + +KKV +QKDVIEL++NIT  AV+H++F+++KL  TR
Sbjct: 62   VKSACENTLHPELCYSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVEKLIKTR 121

Query: 447  KGLTKRQVGALHDCLEMVSETLDELHDVIKDLEEYKTKKTIRRHANDLKTLMSSAITNQE 626
            KGL  R+  ALHDCLE + ETLDELH  IKDLE Y  KK+++ HA+ LKTL+SSAITNQE
Sbjct: 122  KGLKPREKVALHDCLETIDETLDELHTAIKDLELYPNKKSLKAHADGLKTLISSAITNQE 181

Query: 627  TCLDGFSHDGADKKVRKVLIKVEDRVEKMCSNALAMICNMTNTDLANEMKLKG----RNL 794
            TCLDGFSHD ADKKVRK L+K +  VEKMCSNALAMICNMT+TD+ANE KLKG    R L
Sbjct: 182  TCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTDIANEQKLKGTTTNRKL 241

Query: 795  KEAEETVWPEWLSAGDRRLLQSGTVRPNVVVAADGSGNYRTXXXXXXXXPSKSSTRYVIR 974
            +E + + WPEWL AGDRRLLQS TVRP+VVVAADGSGN++T        P KSS RYVIR
Sbjct: 242  RE-DNSEWPEWLPAGDRRLLQSSTVRPDVVVAADGSGNFKTVSEAVAKAPEKSSKRYVIR 300

Query: 975  IKAGVYRENVEVPKAKTKIMFMGDGRRNTIITGSRSVKGGSTTFESATVAIVGDGFLARD 1154
            IKAGVYRENV+VPK KT IMFMGDGR NTIITGSR+VK GSTTF SATVA VG+ FLARD
Sbjct: 301  IKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVGEKFLARD 360

Query: 1155 ITFQNTAGAANHQAVALRVGSDLSAFYQCDMLAYQDTLYVHRNRQFYINCLVVGTVDFIF 1334
            ITFQNTAGAA HQAVALRVGSDLSAFY+CD+LAYQD+LYVH NRQ+++ CL+ GTVDFIF
Sbjct: 361  ITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGTVDFIF 420

Query: 1335 GNAAAVFQDCDLHARRPDSGQKNMFTAQGRTDPNQNTGIVIQKCRIGATSDLLPVQGSFP 1514
            GNAAAV QDCD+HARRP SGQKNM TAQGR+DPNQNTGIVIQKCRIGATSDL PVQ SFP
Sbjct: 421  GNAAAVLQDCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPVQKSFP 480

Query: 1515 TYLGRPWKEYSRTVVMQSSITNVINPAGWFEWNGNFALNTLYYGEYQNTGAGAGTSGRVK 1694
             YLGRPWKEYSRTV+MQSSIT+VIN AGW EWNGNFALNTL+YGEYQNTGAGAGTSGRVK
Sbjct: 481  MYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGTSGRVK 540

Query: 1695 WKGFKVITSSTEAQGFTPGSFLGGGSWLRDTGFPFSLGL 1811
            WKGFKVITS+TEAQ +TPG F+ GGSWL  TGFPFSLGL
Sbjct: 541  WKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579


>gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum]
          Length = 576

 Score =  828 bits (2140), Expect = 0.0
 Identities = 412/574 (71%), Positives = 466/574 (81%), Gaps = 1/574 (0%)
 Frame = +3

Query: 93   DSGKNINLSNKRKKLFLTAFGSXXXXXXXXXXXXXXKSHNSSSDNSTLQSITASAAHAAV 272
            DSGK I+ S ++KK++L    S              KS +++SD+       +S+AHA V
Sbjct: 3    DSGKQIDFSKRKKKIYLAIVASVLLVAAVIGVVAGVKSRSNNSDDDADIMAISSSAHAIV 62

Query: 273  KSSCSSTLYPDLCYSTITSHPELTKKVKTQKDVIELAVNITVTAVEHSYFQIKKLA-TRK 449
            KS+CS+TL+P+LCYS I +  + +KKV +QKDVIEL++NITV AV  +Y+ +K+L  TRK
Sbjct: 63   KSACSNTLHPELCYSAIVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRK 122

Query: 450  GLTKRQVGALHDCLEMVSETLDELHDVIKDLEEYKTKKTIRRHANDLKTLMSSAITNQET 629
            GLT R+  ALHDCLE + ETLDELH  + DLE Y  KK+++ HA DLKTL+SSAITNQET
Sbjct: 123  GLTPREKVALHDCLETMDETLDELHTAVADLELYPNKKSLKEHAEDLKTLISSAITNQET 182

Query: 630  CLDGFSHDGADKKVRKVLIKVEDRVEKMCSNALAMICNMTNTDLANEMKLKGRNLKEAEE 809
            CLDGFSHD ADKKVRKVL+K +  VEKMCSNALAMICNMTNTD+ANEMKL G      + 
Sbjct: 183  CLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTNTDIANEMKLSGSRKLVEDN 242

Query: 810  TVWPEWLSAGDRRLLQSGTVRPNVVVAADGSGNYRTXXXXXXXXPSKSSTRYVIRIKAGV 989
              WPEWLSAGDRRLLQS TV P+VVVAADGSG+Y+T        P KSS RYVIRIKAGV
Sbjct: 243  GEWPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVAKAPEKSSKRYVIRIKAGV 302

Query: 990  YRENVEVPKAKTKIMFMGDGRRNTIITGSRSVKGGSTTFESATVAIVGDGFLARDITFQN 1169
            YRENV+VPK KT IMFMGDGR NTIIT SR+V+ GSTTF SATVA VG+ FLARDITFQN
Sbjct: 303  YRENVDVPKKKTNIMFMGDGRSNTIITASRNVQDGSTTFHSATVAAVGEKFLARDITFQN 362

Query: 1170 TAGAANHQAVALRVGSDLSAFYQCDMLAYQDTLYVHRNRQFYINCLVVGTVDFIFGNAAA 1349
            TAGA+ HQAVALRVGSDLSAFY+CD+LAYQDTLYVH NRQF++ CLV GTVDFIFGN AA
Sbjct: 363  TAGASKHQAVALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAA 422

Query: 1350 VFQDCDLHARRPDSGQKNMFTAQGRTDPNQNTGIVIQKCRIGATSDLLPVQGSFPTYLGR 1529
            V QDCD+HARRP SGQKNM TAQGRTDPNQNTGIVIQKCRIGATSDL PVQ SFPTYLGR
Sbjct: 423  VLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGR 482

Query: 1530 PWKEYSRTVVMQSSITNVINPAGWFEWNGNFALNTLYYGEYQNTGAGAGTSGRVKWKGFK 1709
            PWKEYSRTV+MQSSIT+VI PAGW EWNGNFALNTL+YGEY NTGAGA TSGRVKWKG K
Sbjct: 483  PWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGAATSGRVKWKGHK 542

Query: 1710 VITSSTEAQGFTPGSFLGGGSWLRDTGFPFSLGL 1811
            VITSSTEAQ +TPGSF+ GGSWL  TGFPFSLGL
Sbjct: 543  VITSSTEAQAYTPGSFIAGGSWLSSTGFPFSLGL 576


>gb|AFB77929.1| pectin methylesterase [Gossypium hirsutum]
          Length = 582

 Score =  819 bits (2115), Expect = 0.0
 Identities = 409/589 (69%), Positives = 476/589 (80%), Gaps = 4/589 (0%)
 Frame = +3

Query: 57   MNRIKDFFAGLSDSGKNINLSNKRKKLFLTAFGSXXXXXXXXXXXXXXKSHNSSSDNSTL 236
            M+RIK+  + +S+S K+I+ + K KK+FL  F S               S N+S ++ T 
Sbjct: 1    MSRIKETLSNISNSAKHISFTKKHKKIFLALFASLVIVAAIIGIVAGVSSRNNSDESDT- 59

Query: 237  QSITASAAHAAVKSSCSSTLYPDLCYSTITSHPELT-KKVKTQKDVIELAVNITVTAVEH 413
                  + HA VKS+CS T YPDLC+S +T+ P  T KKV++QKDVIEL++NIT TAVEH
Sbjct: 60   ------SHHAVVKSACSGTFYPDLCFSAVTTVPAGTAKKVRSQKDVIELSLNITTTAVEH 113

Query: 414  SYFQIKKLATRKGLTKRQVGALHDCLEMVSETLDELHDVIKDLEEYKTKKTIRRHANDLK 593
            +YF+IKKL  RK LT R+  ALHDCLE + ETLDELH+ ++DL EY  KK++ +HA+DLK
Sbjct: 114  NYFKIKKLLARKDLTTREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLK 173

Query: 594  TLMSSAITNQETCLDGFSHDGADKKVRKVLIKVEDRVEKMCSNALAMICNMTNTDLANEM 773
            TLMS+A+TNQETCLDGFSH+GADKK+R+VLI  E  VEKMCSNALAMI NMT+TD+ANEM
Sbjct: 174  TLMSAAMTNQETCLDGFSHEGADKKIREVLIDGEKYVEKMCSNALAMIKNMTDTDIANEM 233

Query: 774  KLKGRN--LKEAEETV-WPEWLSAGDRRLLQSGTVRPNVVVAADGSGNYRTXXXXXXXXP 944
             LK  N  LKE E  + WPEWLSAGDRRLLQS +V PNVVVAADGSGN++T        P
Sbjct: 234  MLKSSNRKLKEDESGIAWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFKTVSEAVAKAP 293

Query: 945  SKSSTRYVIRIKAGVYRENVEVPKAKTKIMFMGDGRRNTIITGSRSVKGGSTTFESATVA 1124
             KSS RY+IRIKAGVYRENVEVPK K+ IMF+GDGR  TIITGSR+V  GSTTF SATVA
Sbjct: 294  EKSSKRYIIRIKAGVYRENVEVPKKKSNIMFIGDGRTKTIITGSRNVVDGSTTFHSATVA 353

Query: 1125 IVGDGFLARDITFQNTAGAANHQAVALRVGSDLSAFYQCDMLAYQDTLYVHRNRQFYINC 1304
             VG+ FLARDITFQNTAG + HQAVALRVGSDLSAFY CDMLAYQDTLYVH NRQFY+NC
Sbjct: 354  AVGEKFLARDITFQNTAGPSKHQAVALRVGSDLSAFYNCDMLAYQDTLYVHSNRQFYVNC 413

Query: 1305 LVVGTVDFIFGNAAAVFQDCDLHARRPDSGQKNMFTAQGRTDPNQNTGIVIQKCRIGATS 1484
            LV GTVDFIFGNAAAVFQ+CD+HAR+P+SGQKNM TAQGRTDPNQNTGIVIQKCRIGATS
Sbjct: 414  LVAGTVDFIFGNAAAVFQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 473

Query: 1485 DLLPVQGSFPTYLGRPWKEYSRTVVMQSSITNVINPAGWFEWNGNFALNTLYYGEYQNTG 1664
            DL PV+ +FPTYLGRPWKEYSRTVVMQS+I++VI PAGW EW+G+FAL TL+Y EYQNTG
Sbjct: 474  DLQPVRKNFPTYLGRPWKEYSRTVVMQSTISDVIQPAGWHEWSGSFALKTLFYAEYQNTG 533

Query: 1665 AGAGTSGRVKWKGFKVITSSTEAQGFTPGSFLGGGSWLRDTGFPFSLGL 1811
            AGA TS RVKW G+KVITS++EAQ FTPG F+ GGSWL  TGFPF+LGL
Sbjct: 534  AGASTSARVKWGGYKVITSASEAQAFTPGRFIAGGSWLSSTGFPFALGL 582


>ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vinifera]
          Length = 611

 Score =  814 bits (2102), Expect = 0.0
 Identities = 411/606 (67%), Positives = 479/606 (79%), Gaps = 3/606 (0%)
 Frame = +3

Query: 3    LLEPP--PHCFFLLH*LTKTMNRIKDFFAGLSDSGKNINLSNKRKKLFLTAFGSXXXXXX 176
            L  PP  P   + +      M  +K FF G+SDSGK+ ++S + KKLFL  F S      
Sbjct: 11   LFIPPFSPSIVYTIRHKIPQMPSVKQFFTGMSDSGKHTSISKRNKKLFLLVFSSVLLIAA 70

Query: 177  XXXXXXXXKSHNSSSDNSTLQSITASAAHAAVKSSCSSTLYPDLCYSTITSHPELTKKVK 356
                     S  SS+++  L     +A HA +KS+CSST YPDLCYS I + P  +KKV 
Sbjct: 71   VVGIVAGVHSRKSSTNDVGL-----TAGHAVLKSACSSTRYPDLCYSAIATVPGASKKVT 125

Query: 357  TQKDVIELAVNITVTAVEHSYFQIKKLATRKGLTKRQVGALHDCLEMVSETLDELHDVIK 536
            +QKDVI +++NITVTAVEH+YF I+KL   K LTKR+  ALHDCLE + ETLDELH  + 
Sbjct: 126  SQKDVIAVSLNITVTAVEHNYFTIEKLLDFKNLTKREKAALHDCLETIDETLDELHVAMD 185

Query: 537  DLEEYKTKKTIRRHANDLKTLMSSAITNQETCLDGFSHDGADKKVRKVLIKVEDRVEKMC 716
            DL+EY  KK++ +HA+DLKTLMS+A+TNQETCLDGFSHD ADK VR+VL+K +  VE MC
Sbjct: 186  DLDEYPDKKSLTQHADDLKTLMSAAMTNQETCLDGFSHDDADKHVREVLLKGQRHVEHMC 245

Query: 717  SNALAMICNMTNTDLANEMKLKGRNL-KEAEETVWPEWLSAGDRRLLQSGTVRPNVVVAA 893
            SNALAMI NMT+TD+A E +   R L +E +E+ WP+WLSAGDRRLLQS +V P+VVVAA
Sbjct: 246  SNALAMIKNMTDTDIAREREAMNRKLMEERDESGWPKWLSAGDRRLLQSSSVTPDVVVAA 305

Query: 894  DGSGNYRTXXXXXXXXPSKSSTRYVIRIKAGVYRENVEVPKAKTKIMFMGDGRRNTIITG 1073
            DGSG+Y+T        P KSS RY+I IKAGVY+ENVEV K KT IMF+GDGR NTIITG
Sbjct: 306  DGSGDYKTVSAAVAAAPEKSSKRYIIGIKAGVYKENVEVGKKKTNIMFLGDGRSNTIITG 365

Query: 1074 SRSVKGGSTTFESATVAIVGDGFLARDITFQNTAGAANHQAVALRVGSDLSAFYQCDMLA 1253
            S++V  GSTTF SATVA+VG+ F+ARDITFQNTAG + HQAVALRVGSDLSAFY+CDMLA
Sbjct: 366  SKNVVDGSTTFNSATVAVVGEKFIARDITFQNTAGPSKHQAVALRVGSDLSAFYKCDMLA 425

Query: 1254 YQDTLYVHRNRQFYINCLVVGTVDFIFGNAAAVFQDCDLHARRPDSGQKNMFTAQGRTDP 1433
            YQDTLYVH NRQFYINCLV GTVDFIFGNAAAVFQDCD+HARRP+SGQKNM TAQGRTDP
Sbjct: 426  YQDTLYVHSNRQFYINCLVAGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMLTAQGRTDP 485

Query: 1434 NQNTGIVIQKCRIGATSDLLPVQGSFPTYLGRPWKEYSRTVVMQSSITNVINPAGWFEWN 1613
            NQNTGIVIQKCRIGATSDL  V  SF TYLGRPWKEYSRTVVMQ+SITNVI+PAGW EW+
Sbjct: 486  NQNTGIVIQKCRIGATSDLQAVISSFKTYLGRPWKEYSRTVVMQTSITNVIDPAGWHEWS 545

Query: 1614 GNFALNTLYYGEYQNTGAGAGTSGRVKWKGFKVITSSTEAQGFTPGSFLGGGSWLRDTGF 1793
            G+FAL+TLYYGEYQNTGAGAGTS RV WKGFKVITS++EAQGFTPG+F+ G SWL  TGF
Sbjct: 546  GSFALSTLYYGEYQNTGAGAGTSKRVTWKGFKVITSASEAQGFTPGTFIAGSSWLGSTGF 605

Query: 1794 PFSLGL 1811
            P+SLGL
Sbjct: 606  PYSLGL 611