BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00004453
         (390 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEG79727.1| costunolide synthase [Cichorium intybus]               135   4e-30
gb|AEI59780.1| costunolide synthase [Lactuca sativa]                  134   8e-30
dbj|BAM68819.1| putative cytochrome P450 monooxygenase CYP71AV1 ...   130   8e-29
gb|ABC41927.1| amorpha-4,11-diene monooxygenase [Artemisia annua]     130   1e-28
ref|XP_002513331.1| cytochrome P450, putative [Ricinus communis]...   130   1e-28

>gb|AEG79727.1| costunolide synthase [Cichorium intybus]
          Length = 494

 Score =  135 bits (339), Expect = 4e-30
 Identities = 63/128 (49%), Positives = 89/128 (69%)
 Frame = -2

Query: 389 KEVLKTHDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSSKKVQSFS 210
           +E++KT+DI  ADRP T  S++  Y   D+  +PYGEYWRQMKKIC+LELLS+KKV+SFS
Sbjct: 85  REIMKTNDISFADRPTTTTSQIFFYKAQDIGWAPYGEYWRQMKKICTLELLSAKKVRSFS 144

Query: 209 PIREQELEGFMEFLRLYSGKPINIHKTITELINNVVCKASFGTNCKHQHALLDFLGELAR 30
            IRE+EL    + L   +G PIN  +   E++NNV+CKA+ G +CK Q  L++ L ++ +
Sbjct: 145 SIREEELSRISKVLESQAGTPINFTEMTVEMVNNVICKATLGDSCKDQATLIEVLYDVLK 204

Query: 29  VNSGFYVA 6
             S F +A
Sbjct: 205 TLSAFNLA 212


>gb|AEI59780.1| costunolide synthase [Lactuca sativa]
          Length = 490

 Score =  134 bits (337), Expect = 8e-30
 Identities = 62/128 (48%), Positives = 89/128 (69%)
 Frame = -2

Query: 389 KEVLKTHDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSSKKVQSFS 210
           +E++KT+DI  ADRP T  S++  Y   D+  +PYGEYWRQMKKIC+LELLS+KKV+SFS
Sbjct: 85  REIMKTNDISFADRPTTTTSQIFFYKAQDIGWAPYGEYWRQMKKICTLELLSAKKVRSFS 144

Query: 209 PIREQELEGFMEFLRLYSGKPINIHKTITELINNVVCKASFGTNCKHQHALLDFLGELAR 30
            IRE+EL    + L   +G P+N  +   E++NNV+CKA+ G +CK Q  L++ L ++ +
Sbjct: 145 SIREEELRRISKVLESKAGTPVNFTEMTVEMVNNVICKATLGDSCKDQATLIEVLYDVLK 204

Query: 29  VNSGFYVA 6
             S F +A
Sbjct: 205 TLSAFNLA 212


>dbj|BAM68819.1| putative cytochrome P450 monooxygenase CYP71AV1 [Artemisia
           chamaemelifolia]
          Length = 495

 Score =  130 bits (328), Expect = 8e-29
 Identities = 62/130 (47%), Positives = 90/130 (69%), Gaps = 1/130 (0%)
 Frame = -2

Query: 389 KEVLKTHDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSSKKVQSFS 210
           KE+L T+DI  A RP+T   E+V Y NTD+ALSPYGEYWRQ++KIC+LELLS KKV+SF 
Sbjct: 93  KEILTTYDITFAYRPETLTGEIVAYHNTDIALSPYGEYWRQLRKICTLELLSVKKVKSFQ 152

Query: 209 PIREQELEGFMEFLRLY-SGKPINIHKTITELINNVVCKASFGTNCKHQHALLDFLGELA 33
            +RE+E    ++ ++   SG+P+N+ + + +LI  ++C+A+FG   K Q    +   E+A
Sbjct: 153 SLREEECWNLVQQIKASGSGRPVNLSENVFKLIATILCRAAFGKRIKDQEEFTEIAKEIA 212

Query: 32  RVNSGFYVAD 3
           +   GF VAD
Sbjct: 213 KQAGGFDVAD 222


>gb|ABC41927.1| amorpha-4,11-diene monooxygenase [Artemisia annua]
          Length = 488

 Score =  130 bits (327), Expect = 1e-28
 Identities = 63/130 (48%), Positives = 91/130 (70%), Gaps = 1/130 (0%)
 Frame = -2

Query: 389 KEVLKTHDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSSKKVQSFS 210
           KE+L T+DI  A+RP+T   E+VLY NTDV L+PYGEYWRQ++KIC+LELLS KKV+SF 
Sbjct: 86  KEILTTYDITFANRPETLTGEIVLYHNTDVVLAPYGEYWRQLRKICTLELLSVKKVKSFQ 145

Query: 209 PIREQELEGFMEFLRLY-SGKPINIHKTITELINNVVCKASFGTNCKHQHALLDFLGELA 33
            +RE+E    ++ ++   SG+P+N+ + I +LI  ++ +A+FG   K Q  L + + E+ 
Sbjct: 146 SLREEECWNLVQEIKASGSGRPVNLSENIFKLIATILSRAAFGKGIKDQKELTEIVKEIL 205

Query: 32  RVNSGFYVAD 3
           R   GF VAD
Sbjct: 206 RQTGGFDVAD 215


>ref|XP_002513331.1| cytochrome P450, putative [Ricinus communis]
           gi|223547239|gb|EEF48734.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 506

 Score =  130 bits (327), Expect = 1e-28
 Identities = 60/129 (46%), Positives = 87/129 (67%)
 Frame = -2

Query: 389 KEVLKTHDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSSKKVQSFS 210
           K+VLKT   + A+RP   AS++VLY   D+    YG++WRQM+KIC+ ELLS K+VQSFS
Sbjct: 90  KQVLKTQGELFAERPSILASKIVLYNGMDIIFGSYGDHWRQMRKICTFELLSPKRVQSFS 149

Query: 209 PIREQELEGFMEFLRLYSGKPINIHKTITELINNVVCKASFGTNCKHQHALLDFLGELAR 30
            +R++EL  ++ FL   +G P+N+ KT+  L N+V+ K + G  CK+Q ALL+ + E+  
Sbjct: 150 SVRQEELSNYVRFLHSNAGSPVNLSKTLFALTNSVIAKIAVGKECKNQEALLNLIEEVLV 209

Query: 29  VNSGFYVAD 3
              GF VAD
Sbjct: 210 AAGGFTVAD 218