BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00004452
         (376 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEG79727.1| costunolide synthase [Cichorium intybus]               131   7e-29
gb|AEI59780.1| costunolide synthase [Lactuca sativa]                  130   1e-28
dbj|BAM68819.1| putative cytochrome P450 monooxygenase CYP71AV1 ...   128   6e-28
gb|AFO64615.1| cytochrome P450 mono-oxygenase [Artemisia annua]       127   9e-28
gb|ABE57266.1| amorpha-4,11-diene monooxygenase [Artemisia annua]     127   9e-28

>gb|AEG79727.1| costunolide synthase [Cichorium intybus]
          Length = 494

 Score =  131 bits (329), Expect = 7e-29
 Identities = 62/123 (50%), Positives = 85/123 (69%)
 Frame = -3

Query: 374 THDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSAKKVQSFSPIREQ 195
           T+DI  ADRP T  S++  Y   D+  +PYGEYWRQMKKIC+LELLSAKKV+SFS IRE+
Sbjct: 90  TNDISFADRPTTTTSQIFFYKAQDIGWAPYGEYWRQMKKICTLELLSAKKVRSFSSIREE 149

Query: 194 ELDGFMEFLRLSSGKPINIHKTTTELINNVVCKASFGKNCKHQHALLDFLDELARVNTGF 15
           EL    + L   +G PIN  + T E++NNV+CKA+ G +CK Q  L++ L ++ +  + F
Sbjct: 150 ELSRISKVLESQAGTPINFTEMTVEMVNNVICKATLGDSCKDQATLIEVLYDVLKTLSAF 209

Query: 14  YVA 6
            +A
Sbjct: 210 NLA 212


>gb|AEI59780.1| costunolide synthase [Lactuca sativa]
          Length = 490

 Score =  130 bits (326), Expect = 1e-28
 Identities = 61/123 (49%), Positives = 85/123 (69%)
 Frame = -3

Query: 374 THDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSAKKVQSFSPIREQ 195
           T+DI  ADRP T  S++  Y   D+  +PYGEYWRQMKKIC+LELLSAKKV+SFS IRE+
Sbjct: 90  TNDISFADRPTTTTSQIFFYKAQDIGWAPYGEYWRQMKKICTLELLSAKKVRSFSSIREE 149

Query: 194 ELDGFMEFLRLSSGKPINIHKTTTELINNVVCKASFGKNCKHQHALLDFLDELARVNTGF 15
           EL    + L   +G P+N  + T E++NNV+CKA+ G +CK Q  L++ L ++ +  + F
Sbjct: 150 ELRRISKVLESKAGTPVNFTEMTVEMVNNVICKATLGDSCKDQATLIEVLYDVLKTLSAF 209

Query: 14  YVA 6
            +A
Sbjct: 210 NLA 212


>dbj|BAM68819.1| putative cytochrome P450 monooxygenase CYP71AV1 [Artemisia
           chamaemelifolia]
          Length = 495

 Score =  128 bits (321), Expect = 6e-28
 Identities = 61/125 (48%), Positives = 87/125 (69%), Gaps = 1/125 (0%)
 Frame = -3

Query: 374 THDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSAKKVQSFSPIREQ 195
           T+DI  A RP+T   E+V Y NTD+ALSPYGEYWRQ++KIC+LELLS KKV+SF  +RE+
Sbjct: 98  TYDITFAYRPETLTGEIVAYHNTDIALSPYGEYWRQLRKICTLELLSVKKVKSFQSLREE 157

Query: 194 ELDGFMEFLRLS-SGKPINIHKTTTELINNVVCKASFGKNCKHQHALLDFLDELARVNTG 18
           E    ++ ++ S SG+P+N+ +   +LI  ++C+A+FGK  K Q    +   E+A+   G
Sbjct: 158 ECWNLVQQIKASGSGRPVNLSENVFKLIATILCRAAFGKRIKDQEEFTEIAKEIAKQAGG 217

Query: 17  FYVAD 3
           F VAD
Sbjct: 218 FDVAD 222


>gb|AFO64615.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 488

 Score =  127 bits (319), Expect = 9e-28
 Identities = 61/125 (48%), Positives = 88/125 (70%), Gaps = 1/125 (0%)
 Frame = -3

Query: 374 THDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSAKKVQSFSPIREQ 195
           T+DI  A+RP+T   E+VLY NTDV L+PYGEYWRQ++KIC+LELLS KKV+SF  +RE+
Sbjct: 91  TYDITFANRPETLTGEIVLYHNTDVVLAPYGEYWRQLRKICTLELLSVKKVKSFQSLREE 150

Query: 194 ELDGFMEFLRLS-SGKPINIHKTTTELINNVVCKASFGKNCKHQHALLDFLDELARVNTG 18
           E    ++ ++ S SG+P+N+ +   +LI  ++ +A+FGK  K Q  L + + E+ R   G
Sbjct: 151 ECWNLVQEIKASGSGRPVNLSENVFKLIATILSRAAFGKGIKDQKELTEIVKEILRQTGG 210

Query: 17  FYVAD 3
           F VAD
Sbjct: 211 FDVAD 215


>gb|ABE57266.1| amorpha-4,11-diene monooxygenase [Artemisia annua]
          Length = 488

 Score =  127 bits (319), Expect = 9e-28
 Identities = 61/125 (48%), Positives = 88/125 (70%), Gaps = 1/125 (0%)
 Frame = -3

Query: 374 THDIVLADRPKTFASELVLYGNTDVALSPYGEYWRQMKKICSLELLSAKKVQSFSPIREQ 195
           T+DI  A+RP+T   E+VLY NTDV L+PYGEYWRQ++KIC+LELLS KKV+SF  +RE+
Sbjct: 91  TYDITFANRPETLTGEIVLYHNTDVVLAPYGEYWRQLRKICTLELLSVKKVKSFQSLREE 150

Query: 194 ELDGFMEFLRLS-SGKPINIHKTTTELINNVVCKASFGKNCKHQHALLDFLDELARVNTG 18
           E    ++ ++ S SG+P+N+ +   +LI  ++ +A+FGK  K Q  L + + E+ R   G
Sbjct: 151 ECWNLVQEIKASGSGRPVNLSENVFKLIATILSRAAFGKGIKDQKELTEIVKEILRQTGG 210

Query: 17  FYVAD 3
           F VAD
Sbjct: 211 FDVAD 215