BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00004023
         (1253 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFC36356.1| 2-oxoglutarate-dependent dioxygenase [Salvia milt...   394   e-107
ref|XP_002298131.1| 2-oxoglutarate-dependent dioxygenase [Populu...   371   e-100
ref|XP_002284974.1| PREDICTED: gibberellin 20 oxidase 1 [Vitis v...   365   1e-98
emb|CAN80139.1| hypothetical protein VITISV_028513 [Vitis vinifera]   365   1e-98
ref|XP_002298127.1| 2-oxoglutarate-dependent dioxygenase [Populu...   362   8e-98

>gb|AFC36356.1| 2-oxoglutarate-dependent dioxygenase [Salvia miltiorrhiza]
          Length = 316

 Score =  394 bits (1012), Expect = e-107
 Identities = 187/310 (60%), Positives = 236/310 (76%), Gaps = 2/310 (0%)
 Frame = -2

Query: 1174 MHKLPTIDFTNNRNLKPGSSSWLSTSIEATAALEQYGCFIAEYDKVPVELNNAVFQELQH 995
            +HKL  I+FT+ +N  P + SW  T  +   ALE+YGCF+A YDK+  E++   F  LQ 
Sbjct: 6    VHKLGVIEFTD-KNTNPETESWSETCKKVVDALEEYGCFVAVYDKLTHEIHKQAFSSLQQ 64

Query: 994  LFHLPTETKQQNKSTKPLYGYVGQIPFIPLYESMGFDYSNTLDGVGSFTRVMWPNGNEAF 815
            LF LPT+TK QNKS+KPLYGYVGQIPF+PLYESMG D ++T+ G+ +F+ VMW NGN AF
Sbjct: 65   LFDLPTQTKVQNKSSKPLYGYVGQIPFLPLYESMGIDDAHTIQGIQNFSNVMWHNGNPAF 124

Query: 814  SATLLAYNKLVAELEEMVTRMVFERYGVEKHLDGHRKMVTYLCRGMKYRGPEKNETNMGF 635
               L+ Y KL AE+E +  RMV E YGV KH D + +   YLCR MKYR P+  E  M F
Sbjct: 125  DENLVVYTKLAAEVERIAVRMVCESYGVGKHYDSYVESANYLCRVMKYREPKIGENKMAF 184

Query: 634  VAHTDKDFITVLHQNQVNGLEVKARDGQWFTV-DLLPSSYIVMSGDAAMAWSNDRLHSPY 458
            V HTDK F++ +HQNQV+GL++KA+DG+WFTV  L PSS IVM+GDA MAWSN R+ SP+
Sbjct: 185  VTHTDKSFMSTIHQNQVDGLQIKAKDGEWFTVHHLSPSSIIVMAGDAIMAWSNKRIKSPH 244

Query: 457  HRVTMNGKESRYSIAQFSFLE-GIIETPEELVDEEHPLQFKPFDHLKYLDFYNREENRKL 281
            HRVTM G E+RYSIAQFSF+E  ++ TP+E VDE+HPLQ+KPFDHLK+LDF+++EENR+L
Sbjct: 245  HRVTMEGNEARYSIAQFSFMEKDMVATPDEFVDEDHPLQYKPFDHLKFLDFFSQEENRRL 304

Query: 280  ESAIRTYCGV 251
            ESAI TYCGV
Sbjct: 305  ESAITTYCGV 314


>ref|XP_002298131.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
            gi|222845389|gb|EEE82936.1| 2-oxoglutarate-dependent
            dioxygenase [Populus trichocarpa]
          Length = 313

 Score =  371 bits (952), Expect = e-100
 Identities = 179/311 (57%), Positives = 231/311 (74%), Gaps = 1/311 (0%)
 Frame = -2

Query: 1180 LNMHKLPTIDFTNNRNLKPGSSSWLSTSIEATAALEQYGCFIAEYDKVPVELNNAVFQEL 1001
            L+  +LP IDF+   N+KPG+SSWLST  +   ALE+YGCF+A YDKVP+ L+N +F  L
Sbjct: 4    LSPPELPVIDFSKE-NMKPGTSSWLSTCKDVMYALEEYGCFVAVYDKVPLTLHNDLFSTL 62

Query: 1000 QHLFHLPTETKQQNKSTKPLYGYVGQIPFIP-LYESMGFDYSNTLDGVGSFTRVMWPNGN 824
            + LF LP ETK++N S  P YGYVG  PFIP LYE MG D + TL+G   F   MWP GN
Sbjct: 63   KDLFDLPIETKRKNVSDLPYYGYVGNQPFIPSLYEGMGIDNAITLEGTEDFADTMWPGGN 122

Query: 823  EAFSATLLAYNKLVAELEEMVTRMVFERYGVEKHLDGHRKMVTYLCRGMKYRGPEKNETN 644
            + F  T+L+Y+ LV+ELE+MV RMVFE YGV +H D H++  TYL R MKYRGPEKNE N
Sbjct: 123  DHFCKTVLSYSMLVSELEQMVVRMVFESYGVYQHHDSHKESTTYLFRMMKYRGPEKNEAN 182

Query: 643  MGFVAHTDKDFITVLHQNQVNGLEVKARDGQWFTVDLLPSSYIVMSGDAAMAWSNDRLHS 464
            +G  AHTDK FITVLHQNQV+GLEVK++DGQW      PSS+IVM+G+A + WSNDR+++
Sbjct: 183  VGCDAHTDKSFITVLHQNQVSGLEVKSKDGQWMGFKPSPSSFIVMAGEALLGWSNDRINA 242

Query: 463  PYHRVTMNGKESRYSIAQFSFLEGIIETPEELVDEEHPLQFKPFDHLKYLDFYNREENRK 284
            P+HRV M+  E RYS+  FSF++GII+ PE+L+DE++PL+FKPF++   L ++  EE R 
Sbjct: 243  PFHRVVMSESEPRYSVGLFSFIKGIIKVPEKLIDEKNPLRFKPFENFGLLRYFFTEEGRG 302

Query: 283  LESAIRTYCGV 251
             ES I+ YCGV
Sbjct: 303  KESTIKAYCGV 313


>ref|XP_002284974.1| PREDICTED: gibberellin 20 oxidase 1 [Vitis vinifera]
            gi|297743336|emb|CBI36203.3| unnamed protein product
            [Vitis vinifera]
          Length = 311

 Score =  365 bits (938), Expect = 1e-98
 Identities = 174/306 (56%), Positives = 222/306 (72%)
 Frame = -2

Query: 1168 KLPTIDFTNNRNLKPGSSSWLSTSIEATAALEQYGCFIAEYDKVPVELNNAVFQELQHLF 989
            +LP ++F+    LKPG+ SWL    +   ALE+YGCF+A YDKV  EL+N  F +L+ LF
Sbjct: 7    RLPVLNFSTG-TLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQLEELF 65

Query: 988  HLPTETKQQNKSTKPLYGYVGQIPFIPLYESMGFDYSNTLDGVGSFTRVMWPNGNEAFSA 809
             LPTETK +N S KP +GY+GQ P IPL+ESMG + + TL GV SFT +MWP GN+ F  
Sbjct: 66   DLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAGNDHFCE 125

Query: 808  TLLAYNKLVAELEEMVTRMVFERYGVEKHLDGHRKMVTYLCRGMKYRGPEKNETNMGFVA 629
            T+L+Y+KLV+ELE+MV +MVFE Y  EK+ D H +  TYL R +KYRGP+KNET +G   
Sbjct: 126  TVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIGTSC 185

Query: 628  HTDKDFITVLHQNQVNGLEVKARDGQWFTVDLLPSSYIVMSGDAAMAWSNDRLHSPYHRV 449
            HTDK FITVLHQN+V GLE++ +DG+W +    PSSY+VM+GDA +AWSN R+HSP HRV
Sbjct: 186  HTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGRIHSPEHRV 245

Query: 448  TMNGKESRYSIAQFSFLEGIIETPEELVDEEHPLQFKPFDHLKYLDFYNREENRKLESAI 269
             MNG ++RYSI  F++  GIIE PEEL DEEHPLQFKPF+H   L FY   +  + ES  
Sbjct: 246  IMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNKAGQAESTA 305

Query: 268  RTYCGV 251
            + YCGV
Sbjct: 306  KAYCGV 311


>emb|CAN80139.1| hypothetical protein VITISV_028513 [Vitis vinifera]
          Length = 311

 Score =  365 bits (938), Expect = 1e-98
 Identities = 174/306 (56%), Positives = 222/306 (72%)
 Frame = -2

Query: 1168 KLPTIDFTNNRNLKPGSSSWLSTSIEATAALEQYGCFIAEYDKVPVELNNAVFQELQHLF 989
            +LP ++F+    LKPG+ SWL    +   ALE+YGCF+A YDKV  EL+N  F +L+ LF
Sbjct: 7    RLPVLNFSTG-TLKPGTRSWLLACKDVLHALEEYGCFVAVYDKVSSELDNTTFCQLEELF 65

Query: 988  HLPTETKQQNKSTKPLYGYVGQIPFIPLYESMGFDYSNTLDGVGSFTRVMWPNGNEAFSA 809
             LPTETK +N S KP +GY+GQ P IPL+ESMG + + TL GV SFT +MWP GN+ F  
Sbjct: 66   DLPTETKVKNTSDKPYFGYIGQHPLIPLHESMGIEDATTLKGVESFTNIMWPAGNDHFCE 125

Query: 808  TLLAYNKLVAELEEMVTRMVFERYGVEKHLDGHRKMVTYLCRGMKYRGPEKNETNMGFVA 629
            T+L+Y+KLV+ELE+MV +MVFE Y  EK+ D H +  TYL R +KYRGP+KNET +G   
Sbjct: 126  TVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKNETCIGTSC 185

Query: 628  HTDKDFITVLHQNQVNGLEVKARDGQWFTVDLLPSSYIVMSGDAAMAWSNDRLHSPYHRV 449
            HTDK FITVLHQN+V GLE++ +DG+W +    PSSY+VM+GDA +AWSN R+HSP HRV
Sbjct: 186  HTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGRIHSPEHRV 245

Query: 448  TMNGKESRYSIAQFSFLEGIIETPEELVDEEHPLQFKPFDHLKYLDFYNREENRKLESAI 269
             MNG ++RYSI  F++  GIIE PEEL DEEHPLQFKPF+H   L FY   +  + ES  
Sbjct: 246  IMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNKAGQTESTA 305

Query: 268  RTYCGV 251
            + YCGV
Sbjct: 306  KAYCGV 311


>ref|XP_002298127.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
            gi|222845385|gb|EEE82932.1| 2-oxoglutarate-dependent
            dioxygenase [Populus trichocarpa]
          Length = 313

 Score =  362 bits (930), Expect = 8e-98
 Identities = 174/306 (56%), Positives = 222/306 (72%)
 Frame = -2

Query: 1168 KLPTIDFTNNRNLKPGSSSWLSTSIEATAALEQYGCFIAEYDKVPVELNNAVFQELQHLF 989
            KLP +DF    +LKPG+SSWL    +   ALE+YGCF+ EY+++P+EL + VF  L+ LF
Sbjct: 9    KLPILDFAKE-DLKPGTSSWLKACSDVRLALEEYGCFVVEYNRLPLELRDKVFGVLKELF 67

Query: 988  HLPTETKQQNKSTKPLYGYVGQIPFIPLYESMGFDYSNTLDGVGSFTRVMWPNGNEAFSA 809
             LPTETK QNK  KPL GYVGQI  +PL+ESMG D + +LD   +FT +MWPNGN+ F  
Sbjct: 68   DLPTETKMQNKYEKPLNGYVGQIAKLPLHESMGIDNATSLDATQNFTDLMWPNGNDRFCE 127

Query: 808  TLLAYNKLVAELEEMVTRMVFERYGVEKHLDGHRKMVTYLCRGMKYRGPEKNETNMGFVA 629
             +L Y  L AEL+ MVTRMVFE YGVEK+ D + +  TYL R +K R P+ +E N+GFV 
Sbjct: 128  CVLKYANLAAELDRMVTRMVFESYGVEKYHDSYVESTTYLLRLLKNRPPKVDEHNLGFVT 187

Query: 628  HTDKDFITVLHQNQVNGLEVKARDGQWFTVDLLPSSYIVMSGDAAMAWSNDRLHSPYHRV 449
            HTDK F T+LHQN++NGLEV  +DG+   V+L PSS++V++GDA MAWSNDR+ SP HRV
Sbjct: 188  HTDKSFTTILHQNEINGLEVDTKDGKKINVELSPSSFVVIAGDALMAWSNDRVISPSHRV 247

Query: 448  TMNGKESRYSIAQFSFLEGIIETPEELVDEEHPLQFKPFDHLKYLDFYNREENRKLESAI 269
             MNGK  RYS+A F+F +GI++ PEELVDEEHPL +KP DH+  L FY  EE  K +  I
Sbjct: 248  IMNGKIDRYSLALFAFNKGILQVPEELVDEEHPLMYKPLDHIGLLHFYRTEEGYKSKCPI 307

Query: 268  RTYCGV 251
            + YCGV
Sbjct: 308  KAYCGV 313