BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00027100
         (1561 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267422.2| PREDICTED: LOW QUALITY PROTEIN: abietadienol...   577   e-162
emb|CBI26404.3| unnamed protein product [Vitis vinifera]              576   e-162
ref|XP_002331913.1| cytochrome P450 [Populus trichocarpa] gi|222...   565   e-159
ref|XP_002887479.1| cytochrome P450 family protein [Arabidopsis ...   556   e-156
gb|AAG30983.1|AC012396_19 steroid 22-alpha-hydroxylase, putative...   546   e-153

>ref|XP_002267422.2| PREDICTED: LOW QUALITY PROTEIN: abietadienol/abietadienal
            oxidase-like [Vitis vinifera]
          Length = 500

 Score =  577 bits (1488), Expect = e-162
 Identities = 301/481 (62%), Positives = 353/481 (73%), Gaps = 20/481 (4%)
 Frame = +2

Query: 2    FVIYVAKSV-NLKRNKG--------RLPPGGRGWPVVGDXXXXXXXXXXXNPTTFLQQQV 154
            F+ + AK V N K+N G        ++PPG RGWP++G            +P  F+ QQ 
Sbjct: 17   FIWFAAKFVINKKKNVGGERSSVVYKMPPGSRGWPLIGHSLSWYNSVAGPHPPRFVHQQA 76

Query: 155  LRYGKIFSSNVFGIRAVVSGDPEFNRYVMHNEGKLFKSSYPKSFRDLVGANGVITAQGDQ 334
            L++GKIFS ++FG  AVVS D  FNR++M NEGKLF+SSYPKSFRDLVG NGVIT QG+Q
Sbjct: 77   LKFGKIFSCSLFGKWAVVSADSGFNRFIMQNEGKLFQSSYPKSFRDLVGKNGVITVQGEQ 136

Query: 335  HRKLHMIASNMMRSDILKSRFLTDIQVVMHKTFTNFHHNQTILLQDVCRK---------- 484
             RKLH IASNMMR + LK  FL DIQ++M +T  NF +NQ ILLQDVCRK          
Sbjct: 137  QRKLHSIASNMMRLEKLKFHFLKDIQMIMLQTLGNFANNQVILLQDVCRKAKSDTXIIVQ 196

Query: 485  LAINQMVNQLLGVSSESEVGEMTQLFSAFVDGCLSLPISFPGFAYHTAMXXXXXXXXXXX 664
            +AIN MVNQLLGVS+ESE+ +M QLFS FVDGCLS+PI+FPGFA+HTAM           
Sbjct: 197  VAINLMVNQLLGVSTESEINQMAQLFSDFVDGCLSIPINFPGFAFHTAMKAREAIISKIN 256

Query: 665  XXXXETRHDD-SXXXXXXXXXXXXXXXSLSDDAIADFIINLLFAGYETTAKTMLFAIYYL 841
                  R  + S               SL DDA+ADFIINLLFAG ETTAKTMLFA+Y+L
Sbjct: 257  RIIQMRRQQNLSSKEGDGVLGRLIEEESLGDDAVADFIINLLFAGNETTAKTMLFAVYFL 316

Query: 842  TGSPGAANQLLEEHQNIRLKRPDSGGENDMLTWEEYKSMSFTQNVIDETLRLGGIAIWLM 1021
            T  P A  QLL+EH +IR  RP +G E ++LTW++YK+M FTQ VIDETLRLGGIAIWLM
Sbjct: 317  THCPRAMKQLLDEHCSIR-SRPING-EEELLTWQDYKAMPFTQCVIDETLRLGGIAIWLM 374

Query: 1022 REATEDVEYQDYVIPKGSFVVPFLSAVHRDENFHEEATTFNPWRWMDPENQEKRNWRNSP 1201
            REA EDV+YQDYVIPKG FVVPFLSAVH DEN ++ A +FNPWRWM+PENQEKRNWR+SP
Sbjct: 375  REAKEDVKYQDYVIPKGCFVVPFLSAVHLDENIYKGALSFNPWRWMEPENQEKRNWRSSP 434

Query: 1202 FYSPFGGGSRLCPGAELARLQIALFLHYFVISYRWSQVKEDRMSFFPSARLVNGFPIQLS 1381
             YSPFGGG+R CPGAELARLQIALFLHYFV +YRW Q+KEDRMSFFPSARLVNGF I L+
Sbjct: 435  LYSPFGGGARFCPGAELARLQIALFLHYFVTTYRWRQMKEDRMSFFPSARLVNGFQICLT 494

Query: 1382 K 1384
            +
Sbjct: 495  R 495


>emb|CBI26404.3| unnamed protein product [Vitis vinifera]
          Length = 486

 Score =  576 bits (1485), Expect = e-162
 Identities = 297/471 (63%), Positives = 349/471 (74%), Gaps = 10/471 (2%)
 Frame = +2

Query: 2    FVIYVAKSV-NLKRNKG--------RLPPGGRGWPVVGDXXXXXXXXXXXNPTTFLQQQV 154
            F+ + AK V N K+N G        ++PPG RGWP++G            +P  F+ QQ 
Sbjct: 17   FIWFAAKFVINKKKNVGGERSSVVYKMPPGSRGWPLIGHSLSWYNSVAGPHPPRFVHQQA 76

Query: 155  LRYGKIFSSNVFGIRAVVSGDPEFNRYVMHNEGKLFKSSYPKSFRDLVGANGVITAQGDQ 334
            L++GKIFS ++FG  AVVS D  FNR++M NEGKLF+SSYPKSFRDLVG NGVIT QG+Q
Sbjct: 77   LKFGKIFSCSLFGKWAVVSADSGFNRFIMQNEGKLFQSSYPKSFRDLVGKNGVITVQGEQ 136

Query: 335  HRKLHMIASNMMRSDILKSRFLTDIQVVMHKTFTNFHHNQTILLQDVCRKLAINQMVNQL 514
             RKLH IASNMMR + LK  FL DIQ++M +T  NF +NQ ILLQDVCRK+AIN MVNQL
Sbjct: 137  QRKLHSIASNMMRLEKLKFHFLKDIQMIMLQTLGNFANNQVILLQDVCRKVAINLMVNQL 196

Query: 515  LGVSSESEVGEMTQLFSAFVDGCLSLPISFPGFAYHTAMXXXXXXXXXXXXXXXETRHDD 694
            LGVS+ESE+ +M QLFS FVDGCLS+PI+FPGFA+HTAM                 R  +
Sbjct: 197  LGVSTESEINQMAQLFSDFVDGCLSIPINFPGFAFHTAMKAREAIISKINRIIQMRRQQN 256

Query: 695  -SXXXXXXXXXXXXXXXSLSDDAIADFIINLLFAGYETTAKTMLFAIYYLTGSPGAANQL 871
             S               SL DDA+ADFIINLLFAG ETTAKTMLFA+Y+LT  P A  QL
Sbjct: 257  LSSKEGDGVLGRLIEEESLGDDAVADFIINLLFAGNETTAKTMLFAVYFLTHCPRAMKQL 316

Query: 872  LEEHQNIRLKRPDSGGENDMLTWEEYKSMSFTQNVIDETLRLGGIAIWLMREATEDVEYQ 1051
            L     + + RP +G E ++LTW++YK+M FTQ VIDETLRLGGIAIWLMREA EDV+YQ
Sbjct: 317  L-----VIISRPING-EEELLTWQDYKAMPFTQCVIDETLRLGGIAIWLMREAKEDVKYQ 370

Query: 1052 DYVIPKGSFVVPFLSAVHRDENFHEEATTFNPWRWMDPENQEKRNWRNSPFYSPFGGGSR 1231
            DYVIPKG FVVPFLSAVH DEN ++ A +FNPWRWM+PENQEKRNWR+SP YSPFGGG+R
Sbjct: 371  DYVIPKGCFVVPFLSAVHLDENIYKGALSFNPWRWMEPENQEKRNWRSSPLYSPFGGGAR 430

Query: 1232 LCPGAELARLQIALFLHYFVISYRWSQVKEDRMSFFPSARLVNGFPIQLSK 1384
             CPGAELARLQIALFLHYFV +YRW Q+KEDRMSFFPSARLVNGF I L++
Sbjct: 431  FCPGAELARLQIALFLHYFVTTYRWRQMKEDRMSFFPSARLVNGFQICLTR 481


>ref|XP_002331913.1| cytochrome P450 [Populus trichocarpa] gi|222874585|gb|EEF11716.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 491

 Score =  565 bits (1457), Expect = e-159
 Identities = 287/463 (61%), Positives = 342/463 (73%), Gaps = 5/463 (1%)
 Frame = +2

Query: 8    IYVAKSVNLKRNKGRLPPGGRGWPVVGDXXXXXXXXXXXNPTTFLQQQVLRYGKIFSSNV 187
            + V K  + ++   +LPPG RGWP++GD           +P  F+ QQV R+GKIFS ++
Sbjct: 23   LLVCKKKSKEKTSYKLPPGRRGWPLIGDSFNWFNAVAGSHPPQFVHQQVNRFGKIFSCSL 82

Query: 188  FGIRAVVSGDPEFNRYVMHNEGKLFKSSYPKSFRDLVGANGVITAQGDQHRKLHMIASNM 367
            FG  AVVS DP FNR++M NEGKLF+SSYPKSFRDLVG NGVIT  G+Q RKLH IASNM
Sbjct: 83   FGKWAVVSADPTFNRFIMQNEGKLFQSSYPKSFRDLVGKNGVITVHGEQQRKLHGIASNM 142

Query: 368  MRSDILKSRFLTDIQVVMHKTFTNFHHNQTILLQDVCRK-----LAINQMVNQLLGVSSE 532
            MR + LK  FL +IQ++M +T     +NQ ILLQDVCRK     +AIN MVNQLLG SSE
Sbjct: 143  MRLEKLKFHFLDNIQLIMLQTLNKLDNNQVILLQDVCRKASSALVAINLMVNQLLGASSE 202

Query: 533  SEVGEMTQLFSAFVDGCLSLPISFPGFAYHTAMXXXXXXXXXXXXXXXETRHDDSXXXXX 712
            +E+ EM   FS FVDGCLSLPI+ PGFAYHTAM               +   ++S     
Sbjct: 203  TEINEMAHFFSDFVDGCLSLPINIPGFAYHTAMKAREKIIRKINKTIEKHGQEESSEGSN 262

Query: 713  XXXXXXXXXXSLSDDAIADFIINLLFAGYETTAKTMLFAIYYLTGSPGAANQLLEEHQNI 892
                      SL D+A+ADFIINLLFAG ETTAKTMLFA+Y+LT  P A  QLL+E  +I
Sbjct: 263  GVLGRLLEEESLPDNAVADFIINLLFAGNETTAKTMLFAVYFLTRCPKAMQQLLDEQDSI 322

Query: 893  RLKRPDSGGENDMLTWEEYKSMSFTQNVIDETLRLGGIAIWLMREATEDVEYQDYVIPKG 1072
            R    +S GE  MLTW++YK+MSFTQ VIDETLRLGGIAIWLMREA +DV YQDYVIPKG
Sbjct: 323  R---SNSSGEG-MLTWQDYKAMSFTQCVIDETLRLGGIAIWLMREAKQDVVYQDYVIPKG 378

Query: 1073 SFVVPFLSAVHRDENFHEEATTFNPWRWMDPENQEKRNWRNSPFYSPFGGGSRLCPGAEL 1252
             FVVPFLSAVH DEN ++ A+TF+PWRWM+PENQEKRNWR+SPFY PFGGG+R CPGAEL
Sbjct: 379  CFVVPFLSAVHLDENLYKGASTFHPWRWMEPENQEKRNWRSSPFYCPFGGGARFCPGAEL 438

Query: 1253 ARLQIALFLHYFVISYRWSQVKEDRMSFFPSARLVNGFPIQLS 1381
            +RLQIA+FLHYFV ++ W+Q+KEDRMSFFPSARLVNGF I+L+
Sbjct: 439  SRLQIAIFLHYFVTTFTWTQLKEDRMSFFPSARLVNGFQIRLT 481


>ref|XP_002887479.1| cytochrome P450 family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297333320|gb|EFH63738.1| cytochrome P450 family
            protein [Arabidopsis lyrata subsp. lyrata]
          Length = 488

 Score =  556 bits (1432), Expect = e-156
 Identities = 277/452 (61%), Positives = 336/452 (74%), Gaps = 2/452 (0%)
 Frame = +2

Query: 35   KRNKGRLPPGGRGWPVVGDXXXXXXXXXXXNPTTFLQQQVLRYGKIFSSNVFGIRAVVSG 214
            +R   RLPPG RGWP++GD           +P++F+ +Q+ RYG+IFS ++FG  AVVS 
Sbjct: 38   RREPHRLPPGSRGWPLIGDTFAWLNAVSGSHPSSFVDKQIKRYGRIFSCSLFGKWAVVSA 97

Query: 215  DPEFNRYVMHNEGKLFKSSYPKSFRDLVGANGVITAQGDQHRKLHMIASNMMRSDILKSR 394
            DP FNR++M NEGKLF+SSYPKSFRDLVG +GVIT  GDQ R+LH IAS+MMR D LK+ 
Sbjct: 98   DPAFNRFIMQNEGKLFQSSYPKSFRDLVGKDGVITVHGDQQRRLHSIASSMMRHDQLKTH 157

Query: 395  FLTDIQVVMHKTFTNFHHNQTILLQDVCRKLAINQMVNQLLGVSSESEVGEMTQLFSAFV 574
            FL  I VVM +T +NF   + +LLQD+CRK+AI+ MVNQLLGVSSESEV E++QLFS FV
Sbjct: 158  FLEVIPVVMLQTLSNFKDGEVVLLQDICRKVAIHLMVNQLLGVSSESEVDEISQLFSDFV 217

Query: 575  DGCLSLPISFPGFAYHTAMXXXXXXXXXXXXXXXET--RHDDSXXXXXXXXXXXXXXXSL 748
            DGCLS+PI  PGF YH AM               +     + S               SL
Sbjct: 218  DGCLSVPIDLPGFTYHKAMKARKEIIRKINKTVEKLLQNKEASDTAGNGVLGRLLEEESL 277

Query: 749  SDDAIADFIINLLFAGYETTAKTMLFAIYYLTGSPGAANQLLEEHQNIRLKRPDSGGEND 928
             ++++ADFIINLLFAG ETT+KTMLFA+Y+LT  P A  QLL EH  +      +GG   
Sbjct: 278  PNESLADFIINLLFAGNETTSKTMLFAVYFLTHCPKAMTQLLAEHDRL------AGGT-- 329

Query: 929  MLTWEEYKSMSFTQNVIDETLRLGGIAIWLMREATEDVEYQDYVIPKGSFVVPFLSAVHR 1108
             LTW++YK+M FTQ VIDETLRLGGIAIWLMREA EDV YQDYVIPKG FVVPFLSAVH 
Sbjct: 330  -LTWQDYKTMDFTQCVIDETLRLGGIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVHL 388

Query: 1109 DENFHEEATTFNPWRWMDPENQEKRNWRNSPFYSPFGGGSRLCPGAELARLQIALFLHYF 1288
            DE++++E+ +FNPWRW+DPE Q+KRNWR SPFY PFGGG+R CPGAELARLQIALFLHYF
Sbjct: 389  DESYYKESLSFNPWRWLDPETQQKRNWRTSPFYCPFGGGTRFCPGAELARLQIALFLHYF 448

Query: 1289 VISYRWSQVKEDRMSFFPSARLVNGFPIQLSK 1384
            + +YRW+Q+KEDR+SFFPSARLVNGF IQL++
Sbjct: 449  ITTYRWTQLKEDRISFFPSARLVNGFKIQLNR 480


>gb|AAG30983.1|AC012396_19 steroid 22-alpha-hydroxylase, putative [Arabidopsis thaliana]
          Length = 512

 Score =  546 bits (1407), Expect = e-153
 Identities = 278/477 (58%), Positives = 341/477 (71%), Gaps = 27/477 (5%)
 Frame = +2

Query: 35   KRNKGRLPPGGRGWPVVGDXXXXXXXXXXXNPTTFLQQQV-------------------- 154
            +R   RLPPG RGWP++GD           +P++F+++Q+                    
Sbjct: 37   RRAPHRLPPGSRGWPLIGDTFAWLNAVAGSHPSSFVEKQIKKFVSLLCSVLLLILKRPDN 96

Query: 155  -----LRYGKIFSSNVFGIRAVVSGDPEFNRYVMHNEGKLFKSSYPKSFRDLVGANGVIT 319
                 +RYG+IFS ++FG  AVVS DP+FNR++M NEGKLF+SSYPKSFRDLVG +GVIT
Sbjct: 97   SGFNEIRYGRIFSCSLFGKWAVVSADPDFNRFIMQNEGKLFQSSYPKSFRDLVGKDGVIT 156

Query: 320  AQGDQHRKLHMIASNMMRSDILKSRFLTDIQVVMHKTFTNFHHNQTILLQDVCRKLAINQ 499
              GDQ R+LH IAS+MMR D LK+ FL  I VVM +T +NF   + +LLQD+CRK+AI+ 
Sbjct: 157  VHGDQQRRLHSIASSMMRHDQLKTHFLEVIPVVMLQTLSNFKDGEVVLLQDICRKVAIHL 216

Query: 500  MVNQLLGVSSESEVGEMTQLFSAFVDGCLSLPISFPGFAYHTAMXXXXXXXXXXXXXXXE 679
            MVNQLLGVSSESEV EM+QLFS FVDGCLS+PI  PGF Y+ AM               +
Sbjct: 217  MVNQLLGVSSESEVDEMSQLFSDFVDGCLSVPIDLPGFTYNKAMKARKEIIRKINKTIEK 276

Query: 680  TRHDD--SXXXXXXXXXXXXXXXSLSDDAIADFIINLLFAGYETTAKTMLFAIYYLTGSP 853
               +   S               SL ++++ADFIINLLFAG ETT+KTMLFA+Y+LT  P
Sbjct: 277  RLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFAVYFLTHCP 336

Query: 854  GAANQLLEEHQNIRLKRPDSGGENDMLTWEEYKSMSFTQNVIDETLRLGGIAIWLMREAT 1033
             A  QLLEEH  +      +GG   MLTW++YK+M FTQ VIDETLRLGGIAIWLMREA 
Sbjct: 337  KAMTQLLEEHDRL------AGG---MLTWQDYKTMDFTQCVIDETLRLGGIAIWLMREAK 387

Query: 1034 EDVEYQDYVIPKGSFVVPFLSAVHRDENFHEEATTFNPWRWMDPENQEKRNWRNSPFYSP 1213
            EDV YQDYVIPKG FVVPFLSAVH DE++++E+ +FNPWRW+DPE Q+KRNWR SPFY P
Sbjct: 388  EDVSYQDYVIPKGCFVVPFLSAVHLDESYYKESLSFNPWRWLDPETQQKRNWRTSPFYCP 447

Query: 1214 FGGGSRLCPGAELARLQIALFLHYFVISYRWSQVKEDRMSFFPSARLVNGFPIQLSK 1384
            FGGG+R CPGAELARLQIALFLHYF+ +Y+W+Q+KEDR+SFFPSARLVNGF IQL++
Sbjct: 448  FGGGTRFCPGAELARLQIALFLHYFITTYKWTQLKEDRISFFPSARLVNGFKIQLNR 504