BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00012504
         (1838 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   857   0.0  
gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   854   0.0  
emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]       852   0.0  
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   843   0.0  
gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]       842   0.0  

>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  857 bits (2215), Expect = 0.0
 Identities = 414/462 (89%), Positives = 444/462 (96%)
 Frame = -1

Query: 1691 DSKFLNVGVLLIATLFAAKIISAFLFPRSRKRLPPVVKAWPLVGGLFRFLKGPVVMLKQE 1512
            D+K LNVG+LL+ATL  AK+ISA + PRS+KRLPPVVKAWP+VGGL RFLKGPVVML+QE
Sbjct: 5    DNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVVKAWPIVGGLIRFLKGPVVMLRQE 64

Query: 1511 YPKLGSVFTLNLLNKNITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1332
            YPKLGSVFTLNLLNKNITF IGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 65   YPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 124

Query: 1331 SVRQEQFRFFTESLRVTKLKGYVDQMVTEAEEYFSKWGNSGEVDLKYELEHLIILTASRC 1152
            ++RQEQFRFFTE+LRVTKLKGYVDQMVTEAEEYFSKWG SGEVDLKYELEHLIILTASRC
Sbjct: 125  TIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILTASRC 184

Query: 1151 LLGEEVRNKLFDDVSTLFHDLDNGMLPISVIFPYLPIPAHRRRDQARKKLAQIFASIIAA 972
            LLGEEVRNKLFDDVS LFHDLDNGMLPISVIFPYLPIPAHRRRD ARKKLA+IFA+II +
Sbjct: 185  LLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIINS 244

Query: 971  RKQTGKSENDMLQCFIDSKYKNGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLAN 792
            RK+TGK+ENDMLQCFIDSKYK+GRPTTE E+TGLLIAALFAGQHTSSITSTWTG+YLL N
Sbjct: 245  RKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSYLLTN 304

Query: 791  NKYMSAVVEEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFSV 612
            +KYMSAVV+EQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHS+FSV
Sbjct: 305  DKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSEFSV 364

Query: 611  TTKEGKEFDIPKGHIVATSPAFANRLPHIYKDPDTYNPERFNAGREEDKAAGAFSYISFG 432
            TT+EGKE+DI KGHIVATSPAFANRLPHI+K+PDTY+P+RF  GREEDKAAGAFSYISFG
Sbjct: 365  TTREGKEYDISKGHIVATSPAFANRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSYISFG 424

Query: 431  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNA 306
            GGRHGCLGEPFAYLQIKAIWSHLLRNFE EL+SPFPEIDWNA
Sbjct: 425  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA 466


>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  854 bits (2206), Expect = 0.0
 Identities = 411/462 (88%), Positives = 444/462 (96%)
 Frame = -1

Query: 1691 DSKFLNVGVLLIATLFAAKIISAFLFPRSRKRLPPVVKAWPLVGGLFRFLKGPVVMLKQE 1512
            D+K LNVG+LL+ATL  AK+ISA + PRS+KRLPPV+KA P+VGGL RFLKGP+VML+QE
Sbjct: 5    DNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVIKALPIVGGLIRFLKGPIVMLRQE 64

Query: 1511 YPKLGSVFTLNLLNKNITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1332
            YPKLGSVFTLNLLNKNITF IGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 65   YPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 124

Query: 1331 SVRQEQFRFFTESLRVTKLKGYVDQMVTEAEEYFSKWGNSGEVDLKYELEHLIILTASRC 1152
            ++RQEQFRFFTE+LRVTKLKGYVDQMVTEAEEYFSKWG SGEVDLKYELEHLIILTASRC
Sbjct: 125  TIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILTASRC 184

Query: 1151 LLGEEVRNKLFDDVSTLFHDLDNGMLPISVIFPYLPIPAHRRRDQARKKLAQIFASIIAA 972
            LLGEEVRNKLFDDVS LFHDLDNGMLPISVIFPYLPIPAHRRRD ARKKLA+IFA+II +
Sbjct: 185  LLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIINS 244

Query: 971  RKQTGKSENDMLQCFIDSKYKNGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLAN 792
            RK+TGK+ENDMLQCFIDSKYK+GRPTTE E+TGLLIAALFAGQHTSSITSTWTG+YLL N
Sbjct: 245  RKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSYLLTN 304

Query: 791  NKYMSAVVEEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFSV 612
            NKYMSAVV+EQKNLMKKHGNKVDHDILSEM+VLYRCIKEALRLHPPLIMLLRSSHSDFSV
Sbjct: 305  NKYMSAVVDEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSV 364

Query: 611  TTKEGKEFDIPKGHIVATSPAFANRLPHIYKDPDTYNPERFNAGREEDKAAGAFSYISFG 432
            TT+EGKE+DIPKGHIVATSPAFANRLPHI+K+P++Y+P+RF  GREEDKAAGAFSYISFG
Sbjct: 365  TTREGKEYDIPKGHIVATSPAFANRLPHIFKNPESYDPDRFGPGREEDKAAGAFSYISFG 424

Query: 431  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNA 306
            GGRHGCLGEPFAYLQIKAIWSHLLRNFE EL+SPFPEIDWNA
Sbjct: 425  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA 466


>emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]
          Length = 492

 Score =  852 bits (2202), Expect = 0.0
 Identities = 412/462 (89%), Positives = 443/462 (95%)
 Frame = -1

Query: 1691 DSKFLNVGVLLIATLFAAKIISAFLFPRSRKRLPPVVKAWPLVGGLFRFLKGPVVMLKQE 1512
            D+K +NVG+LL+ATL  AK+ISA + PRS+KRLPP VKAWP++GGLFRFLKGPVVML++E
Sbjct: 6    DNKIMNVGLLLVATLVVAKLISALIMPRSKKRLPPSVKAWPVLGGLFRFLKGPVVMLREE 65

Query: 1511 YPKLGSVFTLNLLNKNITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1332
            YPKLGSVFTLNLLNK ITF IGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 66   YPKLGSVFTLNLLNKKITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 125

Query: 1331 SVRQEQFRFFTESLRVTKLKGYVDQMVTEAEEYFSKWGNSGEVDLKYELEHLIILTASRC 1152
            ++RQEQFRFFTESLRVTKLKGYVDQMV EAEEYFSKWG+SGEVDLKYELEHLIILTASRC
Sbjct: 126  TIRQEQFRFFTESLRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRC 185

Query: 1151 LLGEEVRNKLFDDVSTLFHDLDNGMLPISVIFPYLPIPAHRRRDQARKKLAQIFASIIAA 972
            LLGEEVRNKLF+DVS LFHDLDNGMLPISVIFPYL IPAHRRRD ARKKLAQIFA+IIA+
Sbjct: 186  LLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLSIPAHRRRDNARKKLAQIFANIIAS 245

Query: 971  RKQTGKSENDMLQCFIDSKYKNGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLAN 792
            RK+TGK+E+DMLQCFIDSKYK+GRPTTESE+TGLLIAALFAGQHTSSITSTWTGAYLL N
Sbjct: 246  RKRTGKAESDMLQCFIDSKYKDGRPTTESEITGLLIAALFAGQHTSSITSTWTGAYLLCN 305

Query: 791  NKYMSAVVEEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFSV 612
            NKYMSAVV+EQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDF+V
Sbjct: 306  NKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTV 365

Query: 611  TTKEGKEFDIPKGHIVATSPAFANRLPHIYKDPDTYNPERFNAGREEDKAAGAFSYISFG 432
             T+EGKE+DIPKGHIVATSPAFANRLPHIYK+PDTY+P+RF  GREEDK AGAFSYISFG
Sbjct: 366  KTREGKEYDIPKGHIVATSPAFANRLPHIYKNPDTYDPDRFAPGREEDKTAGAFSYISFG 425

Query: 431  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNA 306
            GGRHGCLGEPFAYLQIKAIWSHLLRNFE EL+SPFPEIDWNA
Sbjct: 426  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA 467


>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  843 bits (2179), Expect = 0.0
 Identities = 404/462 (87%), Positives = 440/462 (95%)
 Frame = -1

Query: 1691 DSKFLNVGVLLIATLFAAKIISAFLFPRSRKRLPPVVKAWPLVGGLFRFLKGPVVMLKQE 1512
            D+K LN G+LL+ATL  AK+ISA + PRS+KRLPPV+K+WP++GGL RFLKGPVVML++E
Sbjct: 5    DNKILNAGLLLVATLVVAKLISALIMPRSKKRLPPVIKSWPILGGLLRFLKGPVVMLREE 64

Query: 1511 YPKLGSVFTLNLLNKNITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1332
            YPKLGSVFTLNLLNKNITF +GPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 65   YPKLGSVFTLNLLNKNITFFVGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 124

Query: 1331 SVRQEQFRFFTESLRVTKLKGYVDQMVTEAEEYFSKWGNSGEVDLKYELEHLIILTASRC 1152
            ++RQEQFRFFTESLRV KLKGYVDQMV EAEEYFSKWG+SGEVDLKYELEHLIILTASRC
Sbjct: 125  TIRQEQFRFFTESLRVNKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRC 184

Query: 1151 LLGEEVRNKLFDDVSTLFHDLDNGMLPISVIFPYLPIPAHRRRDQARKKLAQIFASIIAA 972
            LLGEEVRNKLF+DVS LFHDLDNGMLPISVIFPYLPIPAHRRRD ARKKLA+IFA+II +
Sbjct: 185  LLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIDS 244

Query: 971  RKQTGKSENDMLQCFIDSKYKNGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLAN 792
            RK+TGK+E+DMLQCFIDSKYK+GR TTESE+TGLLIAALFAGQHTSSITSTW GAYLL N
Sbjct: 245  RKRTGKAESDMLQCFIDSKYKDGRATTESEITGLLIAALFAGQHTSSITSTWAGAYLLCN 304

Query: 791  NKYMSAVVEEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFSV 612
            NKYMSAVV+EQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDF+V
Sbjct: 305  NKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTV 364

Query: 611  TTKEGKEFDIPKGHIVATSPAFANRLPHIYKDPDTYNPERFNAGREEDKAAGAFSYISFG 432
             T+EGKE+DIPKGHIVATSPAFANRLPH+YK+PDTY+P+RF  GR+EDK AGAFSYISFG
Sbjct: 365  KTREGKEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSYISFG 424

Query: 431  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNA 306
            GGRHGCLGEPFAYLQIKAIWSHLLRNFE EL+SPFPEIDWNA
Sbjct: 425  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA 466


>gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]
          Length = 487

 Score =  842 bits (2176), Expect = 0.0
 Identities = 405/462 (87%), Positives = 440/462 (95%)
 Frame = -1

Query: 1691 DSKFLNVGVLLIATLFAAKIISAFLFPRSRKRLPPVVKAWPLVGGLFRFLKGPVVMLKQE 1512
            D+KFLNVG+LL+ATL  AK++SA + PRS+KRLPPV+ +WP++GGL RFLKGPVVML++E
Sbjct: 5    DNKFLNVGLLLVATLVVAKLMSALIMPRSKKRLPPVINSWPILGGLIRFLKGPVVMLREE 64

Query: 1511 YPKLGSVFTLNLLNKNITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 1332
            YPKLGSVFTLN+LNKNITF IGPEVSAHFFKA E+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 65   YPKLGSVFTLNILNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 124

Query: 1331 SVRQEQFRFFTESLRVTKLKGYVDQMVTEAEEYFSKWGNSGEVDLKYELEHLIILTASRC 1152
            ++RQEQF FFTE+LRVTKLKGYVDQMV EAEEYFSKWG+SGEVDLKYELEHLIILTASRC
Sbjct: 125  TIRQEQFTFFTEALRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRC 184

Query: 1151 LLGEEVRNKLFDDVSTLFHDLDNGMLPISVIFPYLPIPAHRRRDQARKKLAQIFASIIAA 972
            LLG+EV NKLF DVS LFHDLDNGMLPISVIFPYLPIPAHRRRD+ARKKLA+IFA+II +
Sbjct: 185  LLGQEVPNKLFADVSALFHDLDNGMLPISVIFPYLPIPAHRRRDKARKKLAEIFANIINS 244

Query: 971  RKQTGKSENDMLQCFIDSKYKNGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLAN 792
            RK+TGK+ENDMLQCFIDSKYK+GR TTE E+TGLLIAALFAGQHTSSITSTWTGAYLL N
Sbjct: 245  RKRTGKAENDMLQCFIDSKYKDGRATTEGEITGLLIAALFAGQHTSSITSTWTGAYLLCN 304

Query: 791  NKYMSAVVEEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFSV 612
             KYMSAVV+EQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLR SHSDF V
Sbjct: 305  KKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRHSHSDFCV 364

Query: 611  TTKEGKEFDIPKGHIVATSPAFANRLPHIYKDPDTYNPERFNAGREEDKAAGAFSYISFG 432
            TTKEGKE+DIPKGHIVATSPAFANRLPHIYK+PDTY+P+RF+ GREEDKAAGAFSYISFG
Sbjct: 365  TTKEGKEYDIPKGHIVATSPAFANRLPHIYKNPDTYDPDRFSPGREEDKAAGAFSYISFG 424

Query: 431  GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNA 306
            GGRHGCLGEPFAYLQIKAIWSHLLRNFE EL+SPFPEIDWNA
Sbjct: 425  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA 466