BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00010414
         (460 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   213   1e-53
gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      213   2e-53
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   181   5e-44
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   164   9e-39
ref|XP_003524231.1| PREDICTED: cytochrome P450 716B2-like [Glyci...   149   2e-34

>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
           gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
           13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  213 bits (543), Expect = 1e-53
 Identities = 107/153 (69%), Positives = 126/153 (82%)
 Frame = +1

Query: 1   EEVNVYKSVKLYAFELACRLFMSLEEPNHIAKLGSLFNIFLKGIIELPIDFPGTRFYSAK 180
           EEVNV+++VKLYAFELACRLFM+L++PNHIAKLGSLFNIFLKGIIELPID PGTRFYS+K
Sbjct: 165 EEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSK 224

Query: 181 KAAATIRIQLMPIIKERRMAVEEGKVXXXXXXXXXXXXXXDENGKYLTEMEIANTILLLL 360
           KAAA IRI+L  +IK R++ ++EGK               DENG +LTE EI + ILLLL
Sbjct: 225 KAAAAIRIELKKLIKARKLELKEGKASSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLL 284

Query: 361 FAGHDTSAVSITLVMKSLGEHPDVYDKVLREHL 459
           FAGHDTSA+SITL+MK+LGEH DVYDKVL+E L
Sbjct: 285 FAGHDTSALSITLLMKTLGEHSDVYDKVLKEQL 317


>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  213 bits (541), Expect = 2e-53
 Identities = 107/153 (69%), Positives = 126/153 (82%)
 Frame = +1

Query: 1   EEVNVYKSVKLYAFELACRLFMSLEEPNHIAKLGSLFNIFLKGIIELPIDFPGTRFYSAK 180
           EEVNV+++VKLYAFELACRLFM+L++PNHIAKLGSLFNIFLKGIIELPID PGTRFYS+K
Sbjct: 165 EEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSK 224

Query: 181 KAAATIRIQLMPIIKERRMAVEEGKVXXXXXXXXXXXXXXDENGKYLTEMEIANTILLLL 360
           KAAA IRI+L  +IK R++ ++EGK               DENG +LTE EI + ILLLL
Sbjct: 225 KAAAAIRIELKKLIKARKLELKEGKPSSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLL 284

Query: 361 FAGHDTSAVSITLVMKSLGEHPDVYDKVLREHL 459
           FAGHDTSA+SITL+MK+LGEH DVYDKVL+E L
Sbjct: 285 FAGHDTSALSITLLMKALGEHSDVYDKVLKEQL 317


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
           annua]
          Length = 480

 Score =  181 bits (459), Expect = 5e-44
 Identities = 91/153 (59%), Positives = 117/153 (76%), Gaps = 1/153 (0%)
 Frame = +1

Query: 4   EVNVYKSVKLYAFELACRLFMSLEEPNHIAKLGSLFNIFLKGIIELPIDFPGTRFYSAKK 183
           E+ V+++V+ Y FELACRLF+SL++P H+A+LG+LFN FLKG+ ELPI+ PGTRFY AK+
Sbjct: 169 ELKVFETVRPYLFELACRLFLSLDDPKHVAELGTLFNTFLKGLTELPINIPGTRFYRAKR 228

Query: 184 AAATIRIQLMPIIKERRMAV-EEGKVXXXXXXXXXXXXXXDENGKYLTEMEIANTILLLL 360
           AA  I+ QL+ IIK+RR A+ +E +               DENG++L+E EIAN +LLLL
Sbjct: 229 AANAIKKQLIVIIKQRRQALKQEDQSSSFEDLLSHLLVSSDENGRFLSEAEIANNVLLLL 288

Query: 361 FAGHDTSAVSITLVMKSLGEHPDVYDKVLREHL 459
           FAGHDTSAVSITL+MKSL EHP VYD VL+E L
Sbjct: 289 FAGHDTSAVSITLLMKSLAEHPQVYDNVLKEQL 321


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
           vinifera]
          Length = 473

 Score =  164 bits (414), Expect = 9e-39
 Identities = 84/153 (54%), Positives = 109/153 (71%)
 Frame = +1

Query: 1   EEVNVYKSVKLYAFELACRLFMSLEEPNHIAKLGSLFNIFLKGIIELPIDFPGTRFYSAK 180
           EE+ VY  +KLY FELACRLF S+++P HI+KL + FN+FLKGII+ PI+FPGT+F  A 
Sbjct: 164 EEMQVYPIIKLYTFELACRLFASMDDPGHISKLAAHFNVFLKGIIDFPINFPGTKFXYAT 223

Query: 181 KAAATIRIQLMPIIKERRMAVEEGKVXXXXXXXXXXXXXXDENGKYLTEMEIANTILLLL 360
           KAA  IR +L  +  ERR+A+EE                 D +G++LTEMEI + ILLLL
Sbjct: 224 KAANAIREELRVMPMERRVALEEKTASATQDLLSHLLVTADASGRFLTEMEIIDNILLLL 283

Query: 361 FAGHDTSAVSITLVMKSLGEHPDVYDKVLREHL 459
           FAGHDT+A +ITL++K LGE P+VY KVLRE +
Sbjct: 284 FAGHDTTASAITLLIKYLGELPEVYAKVLREKI 316


>ref|XP_003524231.1| PREDICTED: cytochrome P450 716B2-like [Glycine max]
          Length = 486

 Score =  149 bits (376), Expect = 2e-34
 Identities = 78/153 (50%), Positives = 105/153 (68%)
 Frame = +1

Query: 1   EEVNVYKSVKLYAFELACRLFMSLEEPNHIAKLGSLFNIFLKGIIELPIDFPGTRFYSAK 180
           E+V VY  V+LY FELAC LF+S+E+ +HI+KL   F+ FLKGII  P++ PGTRFY A 
Sbjct: 175 EQVCVYPIVQLYTFELACCLFLSIEDSDHISKLSLKFDEFLKGIIGFPLNVPGTRFYRAM 234

Query: 181 KAAATIRIQLMPIIKERRMAVEEGKVXXXXXXXXXXXXXXDENGKYLTEMEIANTILLLL 360
           KAA  IR ++  I+K+R++ +EE +V              D +G+++TEMEI + ILLLL
Sbjct: 235 KAADVIRKEIKMILKKRKVDLEEKRVSPTQDLLSHMLVTSDPSGRFMTEMEILDNILLLL 294

Query: 361 FAGHDTSAVSITLVMKSLGEHPDVYDKVLREHL 459
           FAGHDTS   ++LVMK LG+ P VY+ VL E L
Sbjct: 295 FAGHDTSRSVLSLVMKYLGQLPQVYEHVLEEQL 327