BLASTX nr result
ID: Atractylodes21_contig00007602
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00007602
(1538 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAD47832.1| cytochrome P450 [Nicotiana tabacum] 410 e-115
emb|CAC24711.1| cytochrome P450 [Solanum tuberosum] 409 e-114
sp|A6YIH8.1|C7D55_HYOMU RecName: Full=Premnaspirodiene oxygenase... 400 e-112
gb|ABC69404.1| CYP71D51v2 [Nicotiana tabacum] 398 e-112
gb|ABC69405.1| CYP71D51v3 [Nicotiana tabacum] 398 e-112
>gb|AAD47832.1| cytochrome P450 [Nicotiana tabacum]
Length = 499
Score = 410 bits (1055), Expect(2) = e-115
Identities = 196/395 (49%), Positives = 278/395 (70%), Gaps = 1/395 (0%)
Frame = -3
Query: 1536 IHQLAGLLPHRAFRDLARKHGPIMHVQLGQISSVIVSSPRLAKEALKTHDIALADRPQTF 1357
+H L GL PH RDLA+K+GPIMH+QLG++S V++SSP +AKE LKTHD+A A+RP
Sbjct: 46 LHMLGGL-PHHVLRDLAKKYGPIMHLQLGEVSLVVISSPGMAKEVLKTHDLAFANRPLLV 104
Query: 1356 VGEVVVYGNTNIVLSPYGDYWRQMKKICSLELLSAKKVQSFGAIREQELDGFMEFLRLSS 1177
++ Y +I LSPYG+YWRQM+KIC LELLSAK V+SF +IR+ E+ ++F R S
Sbjct: 105 AAKIFSYNCMDIALSPYGNYWRQMRKICLLELLSAKNVKSFNSIRQDEVHRMIKFFRSSP 164
Query: 1176 GKPINIHETIMQVINSIVCIASFGKYCKHQHALLEFMEELSLVNSGFHVADLFPDYKFLY 997
GKP+N+ + I NS+ C ++FG+ K Q ++ ++++S + GF VAD+FP KFL+
Sbjct: 165 GKPVNVTKRISLFTNSMTCRSAFGQEYKEQDEFVQLVKKVSNLIEGFDVADIFPSLKFLH 224
Query: 996 VVSGLRSQLMKLRNTYDKIFDDILKEHQDRRRG-GEAEGENILEVLLKIKEEGGLEFPVT 820
V++G+++++M N D I ++I+ EH+ + GE+ GE I+ VLL++ +EGGL+FP+T
Sbjct: 225 VLTGMKAKVMNTHNELDAILENIINEHKKTSKSDGESGGEGIIGVLLRLMKEGGLQFPIT 284
Query: 819 NNIIKGIFVDIFGGGADTSSVTIEWAMTEMIRHPDVMKKAQEELRKAFKGKGKIKESDIQ 640
N+ IK I DIFGGG +TSS TI WAM EM+++P V KAQ E+R+ +GK E D++
Sbjct: 285 NDNIKAIISDIFGGGTETSSTTINWAMVEMMKNPSVFSKAQAEVREILRGKETFGEIDVE 344
Query: 639 GLNYLQSVIKETXXXXXXXXXXXXXVCREQCKIDGYDIPIKTKVIINAYACSTDPEYWDD 460
YL+ VIKET CRE+ ++GY IP+KTKV++NA+A DP+YWDD
Sbjct: 345 EFKYLKMVIKETFRLHPPLPLLLPRECREEIDLNGYTIPLKTKVVVNAWAMGRDPKYWDD 404
Query: 459 SESFKPERFEKNTSIDFRGTNHQFIPFGSGRRICP 355
ESFKPERFE N S+D+ G N++++PFGSGRRICP
Sbjct: 405 VESFKPERFEHN-SMDYIGNNYEYLPFGSGRRICP 438
Score = 33.5 bits (75), Expect(2) = e-115
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -2
Query: 292 GSNPMDIDMTEVDGTFAAKKVPLHLIPTSY 203
G NP + D+TE G A+K LHLI T+Y
Sbjct: 465 GINPRNCDLTEAAGAACARKNDLHLIATAY 494
>emb|CAC24711.1| cytochrome P450 [Solanum tuberosum]
Length = 502
Score = 409 bits (1052), Expect(2) = e-114
Identities = 190/395 (48%), Positives = 277/395 (70%), Gaps = 4/395 (1%)
Frame = -3
Query: 1527 LAGLLPHRAFRDLARKHGPIMHVQLGQISSVIVSSPRLAKEALKTHDIALADRPQTFVGE 1348
+AG LPH RDLA+K+GP+MH+QLG++S+V+V+SP +AKE LKTHDIA A RP+ E
Sbjct: 48 MAGGLPHHVLRDLAKKYGPLMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPE 107
Query: 1347 VVVYGNTNIVLSPYGDYWRQMKKICSLELLSAKKVQSFGAIREQELDGFMEFLRLSSGKP 1168
+V Y ++I PYGDYWRQM+KIC LELLSAK V+S+G+IR E+D + F+R SSG+P
Sbjct: 108 IVCYNRSDIAFCPYGDYWRQMRKICVLELLSAKNVRSYGSIRRDEVDRLVNFIRSSSGEP 167
Query: 1167 INIHETIMQVINSIVCIASFGKYCKHQHALLEFMEELSLVNSGFHVADLFPDYKFLYVVS 988
+N E + +S+ C ++FGK K Q ++ ++E+ + GF VAD+FP KFL+V+S
Sbjct: 168 VNFTERLFLFTSSMTCRSAFGKVFKEQDKFIQLIKEVIGLAGGFDVADIFPSLKFLHVLS 227
Query: 987 GLRSQLMKLRNTYDKIFDDILKEHQDR----RRGGEAEGENILEVLLKIKEEGGLEFPVT 820
G++ ++M + D I +D++ EH+ + + G GE++++VL+++ +GGL+FP+T
Sbjct: 228 GMKGKIMNAHHKVDAIVEDVINEHKKKFAIGKTNGALGGEDLIDVLIRLMNDGGLQFPIT 287
Query: 819 NNIIKGIFVDIFGGGADTSSVTIEWAMTEMIRHPDVMKKAQEELRKAFKGKGKIKESDIQ 640
N+ IK I D+F G +TSS T+ WAM +M+++P V+ KAQ E+R+AFK K E+D++
Sbjct: 288 NDNIKAIIFDMFAAGTETSSSTLVWAMVQMMKNPSVIAKAQAEVREAFKDKETFDENDVE 347
Query: 639 GLNYLQSVIKETXXXXXXXXXXXXXVCREQCKIDGYDIPIKTKVIINAYACSTDPEYWDD 460
L YL+ VIKET CRE+ I+GY IP+KTKV++N +A DP+YWDD
Sbjct: 348 ELKYLKLVIKETLRLHPPVPLLVPRECREETDINGYTIPVKTKVMVNVWALGRDPKYWDD 407
Query: 459 SESFKPERFEKNTSIDFRGTNHQFIPFGSGRRICP 355
+ESFKPERFE+ S+DF G N +++PFG GRRICP
Sbjct: 408 AESFKPERFEQR-SVDFVGNNFEYLPFGGGRRICP 441
Score = 31.2 bits (69), Expect(2) = e-114
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -2
Query: 292 GSNPMDIDMTEVDGTFAAKKVPLHLIPTSYAP 197
G P D+++TE+ G AA+K L L+ T Y P
Sbjct: 468 GMEPKDLNLTELVGVTAARKDDLILVATPYEP 499
>sp|A6YIH8.1|C7D55_HYOMU RecName: Full=Premnaspirodiene oxygenase; Short=HPO; AltName:
Full=Cytochrome P450 71D55 gi|151335776|gb|ABS00393.1|
cytochrome P450 hydroxylase [Hyoscyamus muticus]
Length = 502
Score = 400 bits (1029), Expect(2) = e-112
Identities = 186/398 (46%), Positives = 276/398 (69%), Gaps = 4/398 (1%)
Frame = -3
Query: 1536 IHQLAGLLPHRAFRDLARKHGPIMHVQLGQISSVIVSSPRLAKEALKTHDIALADRPQTF 1357
+H + GL PH RDLA+K+GP+MH+QLG++S+V+V+SP +AKE LKTHDIA A RP+
Sbjct: 46 LHMVGGL-PHHVLRDLAKKYGPLMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLL 104
Query: 1356 VGEVVVYGNTNIVLSPYGDYWRQMKKICSLELLSAKKVQSFGAIREQELDGFMEFLRLSS 1177
E+V Y ++I PYGDYWRQM+KIC LE+LSAK V+SF +IR E+ + F+R S+
Sbjct: 105 APEIVCYNRSDIAFCPYGDYWRQMRKICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSST 164
Query: 1176 GKPINIHETIMQVINSIVCIASFGKYCKHQHALLEFMEELSLVNSGFHVADLFPDYKFLY 997
+P+N E + +S+ C ++FGK K Q ++ ++E+ + GF VAD+FP KFL+
Sbjct: 165 SEPVNFTERLFLFTSSMTCRSAFGKVFKEQETFIQLIKEVIGLAGGFDVADIFPSLKFLH 224
Query: 996 VVSGLRSQLMKLRNTYDKIFDDILKEHQDR----RRGGEAEGENILEVLLKIKEEGGLEF 829
V++G+ ++MK + D I +D++ EH+ + G GE++++VLL++ +GGL+F
Sbjct: 225 VLTGMEGKIMKAHHKVDAIVEDVINEHKKNLAMGKTNGALGGEDLIDVLLRLMNDGGLQF 284
Query: 828 PVTNNIIKGIFVDIFGGGADTSSVTIEWAMTEMIRHPDVMKKAQEELRKAFKGKGKIKES 649
P+TN+ IK I D+F G +TSS T+ WAM +M+R+P ++ KAQ E+R+AFKGK E+
Sbjct: 285 PITNDNIKAIIFDMFAAGTETSSSTLVWAMVQMMRNPTILAKAQAEVREAFKGKETFDEN 344
Query: 648 DIQGLNYLQSVIKETXXXXXXXXXXXXXVCREQCKIDGYDIPIKTKVIINAYACSTDPEY 469
D++ L YL+ VIKET CRE+ +I+GY IP+KTKV++N +A DP+Y
Sbjct: 345 DVEELKYLKLVIKETLRLHPPVPLLVPRECREETEINGYTIPVKTKVMVNVWALGRDPKY 404
Query: 468 WDDSESFKPERFEKNTSIDFRGTNHQFIPFGSGRRICP 355
WDD+++FKPERFE+ S+DF G N +++PFG GRRICP
Sbjct: 405 WDDADNFKPERFEQ-CSVDFIGNNFEYLPFGGGRRICP 441
Score = 33.9 bits (76), Expect(2) = e-112
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = -2
Query: 292 GSNPMDIDMTEVDGTFAAKKVPLHLIPTSYAP 197
G P D+D+TE+ G AA+K L L+ T Y P
Sbjct: 468 GMEPKDLDLTELVGVTAARKSDLMLVATPYQP 499
>gb|ABC69404.1| CYP71D51v2 [Nicotiana tabacum]
Length = 514
Score = 398 bits (1023), Expect(2) = e-112
Identities = 188/398 (47%), Positives = 274/398 (68%), Gaps = 4/398 (1%)
Frame = -3
Query: 1536 IHQLAGLLPHRAFRDLARKHGPIMHVQLGQISSVIVSSPRLAKEALKTHDIALADRPQTF 1357
+H + GL PH RDLA+K+GP+MH+QLG++S+V+V+SP AKE LKTHDIA A RP
Sbjct: 58 LHMVGGL-PHHVLRDLAKKYGPLMHLQLGEVSAVVVTSPDTAKEVLKTHDIAFASRPSLL 116
Query: 1356 VGEVVVYGNTNIVLSPYGDYWRQMKKICSLELLSAKKVQSFGAIREQELDGFMEFLRLSS 1177
E+V Y +++ PYGDYWRQM+KIC LE+LSAK V++F +IR E+ + F+R SS
Sbjct: 117 APEIVCYNRSDLAFCPYGDYWRQMRKICVLEVLSAKNVRTFSSIRRNEVLRLINFIRSSS 176
Query: 1176 GKPINIHETIMQVINSIVCIASFGKYCKHQHALLEFMEELSLVNSGFHVADLFPDYKFLY 997
G+PIN+ E I +S+ C ++FG+ K Q ++ ++E+ L+ GF VAD+FP KFL+
Sbjct: 177 GEPINVTERIFLFTSSMTCRSAFGQVFKEQDKFIQLIKEVILLAGGFDVADIFPSLKFLH 236
Query: 996 VVSGLRSQLMKLRNTYDKIFDDILKEHQDR----RRGGEAEGENILEVLLKIKEEGGLEF 829
V+SG++ ++M + D I ++++ EH+ + G GE++++VLL++ +GGL+F
Sbjct: 237 VLSGMKGKIMNAHHKVDAIVENVINEHKKNLAIGKTNGALGGEDLIDVLLRLMNDGGLQF 296
Query: 828 PVTNNIIKGIFVDIFGGGADTSSVTIEWAMTEMIRHPDVMKKAQEELRKAFKGKGKIKES 649
P+TN+ IK I D+F G +TSS TI WAM EM+++P V KAQ E+R+AF+GK E+
Sbjct: 297 PITNDNIKAIIFDMFAAGTETSSSTIVWAMVEMVKNPAVFAKAQAEVREAFRGKETFDEN 356
Query: 648 DIQGLNYLQSVIKETXXXXXXXXXXXXXVCREQCKIDGYDIPIKTKVIINAYACSTDPEY 469
D++ LNYL+ VIKET CRE+ I+GY IP+KTKV++N +A DP+Y
Sbjct: 357 DVEELNYLKLVIKETLRLHPPVPLLLPRECREETNINGYTIPVKTKVMVNVWALGRDPKY 416
Query: 468 WDDSESFKPERFEKNTSIDFRGTNHQFIPFGSGRRICP 355
W+D+E+F PERFE+ S DF G N +++PFG GRRICP
Sbjct: 417 WNDAETFMPERFEQ-CSKDFVGNNFEYLPFGGGRRICP 453
Score = 35.0 bits (79), Expect(2) = e-112
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = -2
Query: 292 GSNPMDIDMTEVDGTFAAKKVPLHLIPTSYAP 197
G P D+D+TE+ G AA+K L+L+ T Y P
Sbjct: 480 GIEPSDLDLTELVGVTAARKSDLYLVATPYQP 511
>gb|ABC69405.1| CYP71D51v3 [Nicotiana tabacum]
Length = 514
Score = 398 bits (1023), Expect(2) = e-112
Identities = 188/398 (47%), Positives = 274/398 (68%), Gaps = 4/398 (1%)
Frame = -3
Query: 1536 IHQLAGLLPHRAFRDLARKHGPIMHVQLGQISSVIVSSPRLAKEALKTHDIALADRPQTF 1357
+H + GL PH RDLA+K+GP+MH+QLG++S+V+V+SP AKE LKTHDIA A RP
Sbjct: 58 LHMVGGL-PHHVLRDLAKKYGPLMHLQLGEVSAVVVTSPDTAKEVLKTHDIAFASRPSLL 116
Query: 1356 VGEVVVYGNTNIVLSPYGDYWRQMKKICSLELLSAKKVQSFGAIREQELDGFMEFLRLSS 1177
E+V Y +++ PYGDYWRQM+KIC LE+LSAK V++F +IR E+ + F+R SS
Sbjct: 117 APEIVCYNRSDLAFCPYGDYWRQMRKICVLEVLSAKNVRTFSSIRRNEVLRLINFIRSSS 176
Query: 1176 GKPINIHETIMQVINSIVCIASFGKYCKHQHALLEFMEELSLVNSGFHVADLFPDYKFLY 997
G+PIN+ E I +S+ C ++FG+ K Q ++ ++E+ L+ GF VAD+FP KFL+
Sbjct: 177 GEPINVTERIFLFTSSMTCRSAFGQVFKEQDKFIQLIKEVILLAGGFDVADIFPSLKFLH 236
Query: 996 VVSGLRSQLMKLRNTYDKIFDDILKEHQDR----RRGGEAEGENILEVLLKIKEEGGLEF 829
V+SG++ ++M + D I ++++ EH+ + G GE++++VLL++ +GGL+F
Sbjct: 237 VLSGMKGKIMNAHHKVDAIVENVINEHKKNLAIGKTNGALGGEDLIDVLLRLMNDGGLQF 296
Query: 828 PVTNNIIKGIFVDIFGGGADTSSVTIEWAMTEMIRHPDVMKKAQEELRKAFKGKGKIKES 649
P+TN+ IK I D+F G +TSS TI WAM EM+++P V KAQ E+R+AF+GK E+
Sbjct: 297 PITNDNIKAIIFDMFAAGTETSSSTIVWAMVEMVKNPAVFAKAQAEVREAFRGKETFDEN 356
Query: 648 DIQGLNYLQSVIKETXXXXXXXXXXXXXVCREQCKIDGYDIPIKTKVIINAYACSTDPEY 469
D++ LNYL+ VIKET CRE+ I+GY IP+KTKV++N +A DP+Y
Sbjct: 357 DVEELNYLKLVIKETLRLHPPVPLLLPRECREETNINGYTIPVKTKVMVNVWALGRDPKY 416
Query: 468 WDDSESFKPERFEKNTSIDFRGTNHQFIPFGSGRRICP 355
W+D+E+F PERFE+ S DF G N +++PFG GRRICP
Sbjct: 417 WNDAETFMPERFEQ-CSKDFVGNNFEYLPFGGGRRICP 453
Score = 35.0 bits (79), Expect(2) = e-112
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = -2
Query: 292 GSNPMDIDMTEVDGTFAAKKVPLHLIPTSYAP 197
G P D+D+TE+ G AA+K L+L+ T Y P
Sbjct: 480 GIEPSDLDLTELVGVTAARKSDLYLVATPYQP 511