BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00006188
         (1829 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADO16182.1| cytochrome P450 mono-oxygenase [Artemisia annua]       650   0.0  
gb|ADO16183.1| cytochrome P450 mono-oxygenase [Artemisia annua]       616   e-174
emb|CBI19823.3| unnamed protein product [Vitis vinifera]              582   e-164
ref|XP_002280472.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   582   e-164
ref|XP_002278300.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   576   e-162

>gb|ADO16182.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 515

 Score =  650 bits (1678), Expect = 0.0
 Identities = 327/487 (67%), Positives = 385/487 (79%), Gaps = 10/487 (2%)
 Frame = +1

Query: 79   KTIINNSRFN-------PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFI 237
            K I N S+ N       PPGP G+PFIGNLHQID S  H SLW LSKSYGP++SL+ GFI
Sbjct: 24   KIIKNKSKSNVHGQFRSPPGPHGMPFIGNLHQIDKSNFHISLWSLSKSYGPVVSLNLGFI 83

Query: 238  PTIVVSSASLAKEVMKTQDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIH 417
            P IVVSSAS+AKE++KTQDL FCSRPS  G  +VSYNGLDV  SPYN+ W++MR+IF ++
Sbjct: 84   PAIVVSSASVAKEILKTQDLTFCSRPSFHGLQRVSYNGLDVALSPYNKNWKEMRRIFTVY 143

Query: 418  LLGPKRVQSFRHIREDEVSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDE 597
            L  PKR+QS R IREDEVS+AM+ IH LALSS HVNLSEI   V   ++ R+GFGKRY E
Sbjct: 144  LFSPKRLQSSRFIREDEVSLAMEKIHGLALSSKHVNLSEIAHIVMSNMVTRIGFGKRY-E 202

Query: 598  DEHKRTTVFRLLSEVQAMMADFFVSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQE 777
            D ++   + RLL E+QA + ++F+SDLWP  P VGL+DR LGK  RLEKC Q  DSF  +
Sbjct: 203  DGYESKEILRLLHELQATLTNYFISDLWPDFPLVGLIDRLLGKFYRLEKCLQGLDSF-TK 261

Query: 778  LIDEHLNPQNAKSHEEEEDFIDILLRLKKDQL---FDLTYDHIKAMLMDVLVAGTDTSSA 948
            LIDEHL+ + +K +EE ED IDILL+L+  QL   F+LT DH+KAML D+LVAGTD S+A
Sbjct: 262  LIDEHLDAEYSKPNEEHEDLIDILLQLRNGQLSDSFELTNDHMKAMLTDILVAGTDNSAA 321

Query: 949  TVVWAMTALIKNPNVMKKAQEEVRNVVGKKGKVDEDDLPKLTYLKAVVKEIMRLYPSVPL 1128
            T+VWAMT L+K P  MKKAQEEVR +V  K KVDEDDLPKLTYLKAVVKE+MRLYP+ PL
Sbjct: 322  TLVWAMTTLVKYPKAMKKAQEEVRKMVQNKDKVDEDDLPKLTYLKAVVKEVMRLYPAAPL 381

Query: 1129 LVPRETTKDAILHGYKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDF 1308
            L+PR TTKDAIL  YKIKQ TLV+VNA+AIGRDPE WE+PEEF PERFLGSDI FKG+DF
Sbjct: 382  LIPRVTTKDAILLDYKIKQNTLVYVNAMAIGRDPESWENPEEFSPERFLGSDIGFKGSDF 441

Query: 1309 ELIPFGAGRRICPGISMGAVTVDLLLANLVYLFDWGLPDGMRMEDVDFEVLPGLTMHKRN 1488
            ELIPFGAGRRICPGISMG  +V+L LANL+Y FDWGLPDG ++ED+D  VLPGLTM  + 
Sbjct: 442  ELIPFGAGRRICPGISMGVNSVELFLANLIYSFDWGLPDGTKIEDIDSGVLPGLTMTNKK 501

Query: 1489 ELCLLAH 1509
             LCLLAH
Sbjct: 502  GLCLLAH 508


>gb|ADO16183.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 491

 Score =  616 bits (1589), Expect = e-174
 Identities = 314/487 (64%), Positives = 365/487 (74%), Gaps = 10/487 (2%)
 Frame = +1

Query: 79   KTIINNSRFN-------PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFI 237
            K I N S+ N       PPGP G+PFIGNLHQID S  H SLW LSKSYGP++SL+ GFI
Sbjct: 24   KIIKNKSKSNVHGQFRSPPGPHGMPFIGNLHQIDKSNFHISLWSLSKSYGPVVSLNLGFI 83

Query: 238  PTIVVSSASLAKEVMKTQDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIH 417
            P IVVSSAS+AKE++KTQDL FCSRPS  G  +VSYNGLDV  SPYN+ W++MR+IF ++
Sbjct: 84   PAIVVSSASVAKEILKTQDLTFCSRPSFHGLQRVSYNGLDVALSPYNKNWKEMRRIFTVY 143

Query: 418  LLGPKRVQSFRHIREDEVSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDE 597
            L  PKR+QS R IREDEVS+AM+ IH LALSS HVNLSEI   V   +  R+GFGKRY+ 
Sbjct: 144  LFSPKRLQSSRFIREDEVSLAMEKIHGLALSSKHVNLSEIAHIVMSNMATRIGFGKRYE- 202

Query: 598  DEHKRTTVFRLLSEVQAMMADFFVSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQE 777
                                     D +   P VGL+DR LGK  RLEKC Q  DSFYQ 
Sbjct: 203  -------------------------DGYESFPLVGLIDRLLGKFYRLEKCLQGLDSFYQN 237

Query: 778  LIDEHLNPQNAKSHEEEEDFIDILLRLKKDQL---FDLTYDHIKAMLMDVLVAGTDTSSA 948
            LIDEHL+ + +K +EE ED IDILL+L+  QL   F+LT DH+KAML D+LVAGTD S+A
Sbjct: 238  LIDEHLDAEYSKPNEEHEDLIDILLQLRNGQLSDSFELTNDHMKAMLTDILVAGTDNSAA 297

Query: 949  TVVWAMTALIKNPNVMKKAQEEVRNVVGKKGKVDEDDLPKLTYLKAVVKEIMRLYPSVPL 1128
            T+VWAMT L+K P  MKKAQEEVR +V  K KVDEDDLPKLTYLKAVVKE+MRLYP+ PL
Sbjct: 298  TLVWAMTTLVKYPKAMKKAQEEVRKMVQNKDKVDEDDLPKLTYLKAVVKEVMRLYPAAPL 357

Query: 1129 LVPRETTKDAILHGYKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDF 1308
            L+PR T KDA L  YKIKQ TLV+VNA+AIGRDPE WE+PEEF PERFLGSDI FKG+DF
Sbjct: 358  LIPRVTMKDATLLDYKIKQNTLVYVNAMAIGRDPESWENPEEFSPERFLGSDIGFKGSDF 417

Query: 1309 ELIPFGAGRRICPGISMGAVTVDLLLANLVYLFDWGLPDGMRMEDVDFEVLPGLTMHKRN 1488
            ELIPFGAGRRICPGISMG  +V+L LANL+Y FDWGLPDG ++ED+D  VLPGLTM  + 
Sbjct: 418  ELIPFGAGRRICPGISMGVNSVELFLANLIYSFDWGLPDGTKIEDIDSGVLPGLTMTNKK 477

Query: 1489 ELCLLAH 1509
            +LCLLAH
Sbjct: 478  DLCLLAH 484


>emb|CBI19823.3| unnamed protein product [Vitis vinifera]
          Length = 912

 Score =  582 bits (1501), Expect = e-164
 Identities = 286/471 (60%), Positives = 361/471 (76%), Gaps = 2/471 (0%)
 Frame = +1

Query: 109  PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVSSASLAKEVMKT 288
            PPGPPGLPFIGNLHQ+D S  H  LW LSK YG ++SL  GFIPTIVVSSA +AKEVMKT
Sbjct: 446  PPGPPGLPFIGNLHQMDNSARHRYLWQLSKQYGSLMSLRLGFIPTIVVSSARIAKEVMKT 505

Query: 289  QDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKRVQSFRHIREDE 468
             DL F SRPSL+G  ++SYN LD+ FSPYN+YWR+MRKI ++HL   KRVQS+  IRE E
Sbjct: 506  HDLEFASRPSLIGPQRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQSYTPIREYE 565

Query: 469  VSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDEHKRTTVFRLLSEVQA 648
            VS  ++ I +LA +S  +NLSE +  +T TI+ RV FGKRY+++  +R+    LL++ QA
Sbjct: 566  VSQMIEKISKLASASKLINLSETLMFLTSTIICRVAFGKRYEDEGFERSRFHGLLNDAQA 625

Query: 649  MMADFFVSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEE 828
            M+  FF SD +P    +G +D+  G   RLEK F+  D FYQE+I++HL P   K  +E+
Sbjct: 626  MLGSFFFSDHFP---LIGWLDKLTGLTARLEKTFRDMDLFYQEIIEDHLKPDRKK--QEQ 680

Query: 829  EDFIDILLRLKKDQLF--DLTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKK 1002
            ED  D+L+ L+KD  F  D+T+DHIK +LM++ V GTDT +ATV+WAMTAL+KNP VMKK
Sbjct: 681  EDITDVLIGLQKDNSFAIDITWDHIKGVLMNIFVGGTDTGAATVIWAMTALMKNPRVMKK 740

Query: 1003 AQEEVRNVVGKKGKVDEDDLPKLTYLKAVVKEIMRLYPSVPLLVPRETTKDAILHGYKIK 1182
            AQEEVRN  GKKG + EDD+ KL YLKAVVKE MRL P+VPLL+PRET +   + GY+I 
Sbjct: 741  AQEEVRNTFGKKGFIGEDDVEKLPYLKAVVKETMRLLPAVPLLIPRETLQKCSIDGYEIP 800

Query: 1183 QKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFELIPFGAGRRICPGISMG 1362
             KTLVFVNA AIGRDPE WE+PEEF+PERFLGS ++F+G +++LIPFGAGRR+CPGI +G
Sbjct: 801  PKTLVFVNAWAIGRDPEAWENPEEFIPERFLGSSVDFRGQNYKLIPFGAGRRVCPGIHIG 860

Query: 1363 AVTVDLLLANLVYLFDWGLPDGMRMEDVDFEVLPGLTMHKRNELCLLAHAY 1515
            AVTV+L LANL+Y FDW +P GM  ED+DF+V+PGLTMHK+N LCL+A  Y
Sbjct: 861  AVTVELTLANLLYSFDWEMPAGMNKEDIDFDVIPGLTMHKKNALCLMAKKY 911



 Score =  402 bits (1034), Expect = e-109
 Identities = 207/364 (56%), Positives = 263/364 (72%), Gaps = 2/364 (0%)
 Frame = +1

Query: 109  PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVSSASLAKEVMKT 288
            PPGPPGLP IGNLHQ+D S  H  LW LSK YGP++SL  GFIPTIVVSSA +AKEVMKT
Sbjct: 32   PPGPPGLPVIGNLHQMDNSAPHRYLWQLSKQYGPLMSLRLGFIPTIVVSSARIAKEVMKT 91

Query: 289  QDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKRVQSFRHIREDE 468
             DL F SRPSL+G  ++SYN LD+ FSPYN+YWR+MRKI ++HL   KRVQS+  IRE E
Sbjct: 92   HDLKFASRPSLIGPRRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQSYTPIREYE 151

Query: 469  VSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDEHKRTTVFRLLSEVQA 648
            VS  ++ I +LA +S  +NLSE +  +T TI+ RV FGKRY+++  + +    LL++ QA
Sbjct: 152  VSQMIEKISKLASASKLINLSETVMFLTITIICRVSFGKRYEDEGCETSRFHGLLNDAQA 211

Query: 649  MMADFFVSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEE 828
            M+  FF SD     P +G +D+  G   RLEK  +  D FYQE+I++HL P   K  +E+
Sbjct: 212  MLGSFFFSD---HFPLMGWLDKLTGLTARLEKTLRDMDLFYQEIIEDHLKPDRKK--QEQ 266

Query: 829  EDFIDILLRLKKDQLF--DLTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKK 1002
            ED  D+L+ L+KD  F  D+T+DHIK +LM++ V GTD  +ATV+WAMTAL+KNP VMKK
Sbjct: 267  EDITDVLIELQKDNSFAIDITWDHIKGVLMNIFVGGTDAGTATVIWAMTALMKNPRVMKK 326

Query: 1003 AQEEVRNVVGKKGKVDEDDLPKLTYLKAVVKEIMRLYPSVPLLVPRETTKDAILHGYKIK 1182
            AQEEVRN  GKKG + EDD+ KL YLKAVVKE MRL P+ PLL+PRET +   + GY+I 
Sbjct: 327  AQEEVRNTFGKKGFIGEDDVEKLPYLKAVVKETMRLLPAAPLLLPRETLQKCSIDGYEIP 386

Query: 1183 QKTL 1194
             KTL
Sbjct: 387  PKTL 390


>ref|XP_002280472.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
            gi|147832399|emb|CAN64422.1| hypothetical protein
            VITISV_032274 [Vitis vinifera]
          Length = 498

 Score =  582 bits (1501), Expect = e-164
 Identities = 286/471 (60%), Positives = 361/471 (76%), Gaps = 2/471 (0%)
 Frame = +1

Query: 109  PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVSSASLAKEVMKT 288
            PPGPPGLPFIGNLHQ+D S  H  LW LSK YG ++SL  GFIPTIVVSSA +AKEVMKT
Sbjct: 32   PPGPPGLPFIGNLHQMDNSARHRYLWQLSKQYGSLMSLRLGFIPTIVVSSARIAKEVMKT 91

Query: 289  QDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKRVQSFRHIREDE 468
             DL F SRPSL+G  ++SYN LD+ FSPYN+YWR+MRKI ++HL   KRVQS+  IRE E
Sbjct: 92   HDLEFASRPSLIGPQRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQSYTPIREYE 151

Query: 469  VSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDEHKRTTVFRLLSEVQA 648
            VS  ++ I +LA +S  +NLSE +  +T TI+ RV FGKRY+++  +R+    LL++ QA
Sbjct: 152  VSQMIEKISKLASASKLINLSETLMFLTSTIICRVAFGKRYEDEGFERSRFHGLLNDAQA 211

Query: 649  MMADFFVSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEE 828
            M+  FF SD +P    +G +D+  G   RLEK F+  D FYQE+I++HL P   K  +E+
Sbjct: 212  MLGSFFFSDHFP---LIGWLDKLTGLTARLEKTFRDMDLFYQEIIEDHLKPDRKK--QEQ 266

Query: 829  EDFIDILLRLKKDQLF--DLTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKK 1002
            ED  D+L+ L+KD  F  D+T+DHIK +LM++ V GTDT +ATV+WAMTAL+KNP VMKK
Sbjct: 267  EDITDVLIGLQKDNSFAIDITWDHIKGVLMNIFVGGTDTGAATVIWAMTALMKNPRVMKK 326

Query: 1003 AQEEVRNVVGKKGKVDEDDLPKLTYLKAVVKEIMRLYPSVPLLVPRETTKDAILHGYKIK 1182
            AQEEVRN  GKKG + EDD+ KL YLKAVVKE MRL P+VPLL+PRET +   + GY+I 
Sbjct: 327  AQEEVRNTFGKKGFIGEDDVEKLPYLKAVVKETMRLLPAVPLLIPRETLQKCSIDGYEIP 386

Query: 1183 QKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFELIPFGAGRRICPGISMG 1362
             KTLVFVNA AIGRDPE WE+PEEF+PERFLGS ++F+G +++LIPFGAGRR+CPGI +G
Sbjct: 387  PKTLVFVNAWAIGRDPEAWENPEEFIPERFLGSSVDFRGQNYKLIPFGAGRRVCPGIHIG 446

Query: 1363 AVTVDLLLANLVYLFDWGLPDGMRMEDVDFEVLPGLTMHKRNELCLLAHAY 1515
            AVTV+L LANL+Y FDW +P GM  ED+DF+V+PGLTMHK+N LCL+A  Y
Sbjct: 447  AVTVELTLANLLYSFDWEMPAGMNKEDIDFDVIPGLTMHKKNALCLMAKKY 497


>ref|XP_002278300.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
          Length = 495

 Score =  576 bits (1485), Expect = e-162
 Identities = 283/468 (60%), Positives = 361/468 (77%), Gaps = 2/468 (0%)
 Frame = +1

Query: 109  PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVSSASLAKEVMKT 288
            PPGPPGLP IGNL Q+D S  H  LW LSK YGP++ L  GF+PT+VVSSA +AKEVMKT
Sbjct: 28   PPGPPGLPLIGNLLQLDKSAPHIYLWRLSKQYGPLMILRLGFVPTLVVSSARMAKEVMKT 87

Query: 289  QDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKRVQSFRHIREDE 468
             DL F  RPSL+G  K+SYNGLDV FSPYN+YWR+MRKI ++HL   KR QSFR IREDE
Sbjct: 88   HDLEFSGRPSLLGLRKLSYNGLDVAFSPYNDYWREMRKICVLHLFNSKRAQSFRPIREDE 147

Query: 469  VSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDEHKRTTVFRLLSEVQA 648
            V   +K I + A +S   NLSEI+ ++T TI+ RV F KRYD++ ++R+   +L+ E QA
Sbjct: 148  VLEMIKKISQFASASKLTNLSEILISLTSTIICRVAFSKRYDDEGYERSRFQKLVGEGQA 207

Query: 649  MMADFFVSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEE 828
            ++  F+ SD +P M   G VD+  G +   +K F+ FD FYQE+IDEHL+P   +   E+
Sbjct: 208  VVGGFYFSDYFPLM---GWVDKLTGMIALADKNFKEFDLFYQEIIDEHLDPN--RPEPEK 262

Query: 829  EDFIDILLRLKKDQLF--DLTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKK 1002
            ED  D+LL+L+K++LF  DLT+DHIKA+LM++ +AGTDTS+AT+VWAMT L+KNP  M K
Sbjct: 263  EDITDVLLKLQKNRLFTIDLTFDHIKAVLMNIFLAGTDTSAATLVWAMTMLMKNPRTMTK 322

Query: 1003 AQEEVRNVVGKKGKVDEDDLPKLTYLKAVVKEIMRLYPSVPLLVPRETTKDAILHGYKIK 1182
            AQEE+RN++GKKG VDEDDL KL YLKA+VKE MRL+P+ PLLVPRET +  ++ GY+I 
Sbjct: 323  AQEELRNLIGKKGFVDEDDLQKLPYLKAIVKETMRLHPASPLLVPRETLEKCVIDGYEIP 382

Query: 1183 QKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFELIPFGAGRRICPGISMG 1362
             KTLV+VNA AIGRDPE WE+PEEF+PERFLG+ I+FKG D++LIPFG GRRICPG+++G
Sbjct: 383  PKTLVYVNAWAIGRDPESWENPEEFMPERFLGTSIDFKGQDYQLIPFGGGRRICPGLNLG 442

Query: 1363 AVTVDLLLANLVYLFDWGLPDGMRMEDVDFEVLPGLTMHKRNELCLLA 1506
            A  V+L LANL+Y FDW +P GM  ED+D +V PG+TMHK+N LCLLA
Sbjct: 443  AAMVELTLANLLYSFDWEMPAGMNKEDIDIDVKPGITMHKKNALCLLA 490