BLASTX nr result
ID: Atractylodes21_contig00006186
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00006186
(1432 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACF49260.1| 12-oxophytodienoate reductase-like protein [Artem... 702 0.0
sp|C5H429.1|DBR2_ARTAN RecName: Full=Artemisinic aldehyde Delta(... 685 0.0
gb|AER51971.1| 12-oxophytodienoate reductase 3 [Solanum tuberosum] 632 e-179
ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lyc... 631 e-178
pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-... 631 e-178
>gb|ACF49260.1| 12-oxophytodienoate reductase-like protein [Artemisia annua]
Length = 392
Score = 702 bits (1813), Expect = 0.0
Identities = 341/382 (89%), Positives = 361/382 (94%)
Frame = +2
Query: 185 AHKPTLFSPYKMGNFNLSHRVVLAPMTRCRALNNTPNEALVEYYRQRATDGGFLITEGTM 364
+ KPTLFSPYKMGNFNLSHRVVLAPMTRCRA+N PNEALVEYYRQR+T GGFLITEGTM
Sbjct: 2 SEKPTLFSPYKMGNFNLSHRVVLAPMTRCRAINAIPNEALVEYYRQRSTAGGFLITEGTM 61
Query: 365 ISPTSAGFPHVPGIFNKEQVEGWKKVVDAVHKEGAVIFCQLWHVGRASHQVYQPGGAAPI 544
ISP+SAGFPHVPGIF KEQVEGWKKVVDA HKEGAVIFCQLWHVGRASHQVYQP GAAPI
Sbjct: 62 ISPSSAGFPHVPGIFTKEQVEGWKKVVDAAHKEGAVIFCQLWHVGRASHQVYQPRGAAPI 121
Query: 545 SSTNKPISKKWRILMADATYNRYPEPRPLAIHEIPEVVEDYRLAAINAIEAGFDGIEIHG 724
SST+KPISKKW+ILM DATY YPEPRPLA +EI EVVEDYR+AAINAIEAGFDGIEIHG
Sbjct: 122 SSTSKPISKKWKILMPDATYGTYPEPRPLAANEILEVVEDYRIAAINAIEAGFDGIEIHG 181
Query: 725 AHGYLLDQFMKDGINDRTDEYGGSLANRCKFLLQVVKAVATAIGTDRVGVRISPAIDHLD 904
AHGYLLDQFMKDGINDRTDEYGGSL NRCKF+LQVV+AV+ AIG DRVG+RISPAIDHLD
Sbjct: 182 AHGYLLDQFMKDGINDRTDEYGGSLENRCKFILQVVQAVSAAIGADRVGLRISPAIDHLD 241
Query: 905 AMDSDPRSLGLAVIERLNKLQFELGSKLSYLHVTQPRYTAYGQTEAGTHGSEEEVAELMK 1084
AMDSDPRSLGLAVIERLNKLQF+LGS+L+YLHVTQPRY AYGQTEAG HGSEEEVA+LMK
Sbjct: 242 AMDSDPRSLGLAVIERLNKLQFKLGSRLAYLHVTQPRYMAYGQTEAGAHGSEEEVAQLMK 301
Query: 1085 TWRHAYVGTFICSGGYTRELGLEAVAKEDADLVSYGRLFISNPDLPLRLKLNAPLNRYVR 1264
TWR AYVGTFICSGGYTRELGL+AVA+ DADLV++GR FISNPDL LRLKLNAPLNRYVR
Sbjct: 302 TWRGAYVGTFICSGGYTRELGLQAVAQGDADLVAFGRHFISNPDLVLRLKLNAPLNRYVR 361
Query: 1265 ATFYTHDPVVGYTDYPSLDKGN 1330
ATFYTHDPVVGYTDYPSLDKGN
Sbjct: 362 ATFYTHDPVVGYTDYPSLDKGN 383
>sp|C5H429.1|DBR2_ARTAN RecName: Full=Artemisinic aldehyde Delta(11(13)) reductase
gi|197310860|gb|ACH61780.1| artemisinic aldehyde
delta-11(13) reductase [Artemisia annua]
Length = 387
Score = 685 bits (1768), Expect = 0.0
Identities = 334/384 (86%), Positives = 358/384 (93%), Gaps = 2/384 (0%)
Frame = +2
Query: 185 AHKPTLFSPYKMGNFNLSHRVVLAPMTRCRALNNTPNEALVEYYRQRATDGGFLITEGTM 364
+ KPTLFS YKMG FNLSHRVVLAPMTRCRA+N PNEALVEYYRQR+T GGFLITEGTM
Sbjct: 2 SEKPTLFSAYKMGKFNLSHRVVLAPMTRCRAINAIPNEALVEYYRQRSTAGGFLITEGTM 61
Query: 365 ISPTSAGFPHVPGIFNKEQVEGWKKVVDAVHKEGAVIFCQLWHVGRASHQVYQPGGAAPI 544
ISP+SAGFPHVPGIF KEQVEGWKKVVDA HKEGAVIFCQLWHVGRASHQVYQPGGAAPI
Sbjct: 62 ISPSSAGFPHVPGIFTKEQVEGWKKVVDAAHKEGAVIFCQLWHVGRASHQVYQPGGAAPI 121
Query: 545 SSTNKPISKKWRILMADATYNRYPEPRPLAIHEIPEVVEDYRLAAINAIEAGFDGIEIHG 724
SST+KPISKKW IL+ DATY YPEPRPLA +EI EVVEDYR+AAINAIEAGFDGIEIHG
Sbjct: 122 SSTSKPISKKWEILLPDATYGTYPEPRPLAANEILEVVEDYRVAAINAIEAGFDGIEIHG 181
Query: 725 AHGYLLDQFMKDGINDRTDEYGGSLANRCKFLLQVVKAVATAIGTDRVGVRISPAIDHLD 904
AHGYLLDQFMKDGINDRTDEYGGSL NRCKF+LQVV+AV+ AIGTDRVG+RISPAIDH D
Sbjct: 182 AHGYLLDQFMKDGINDRTDEYGGSLENRCKFILQVVQAVSAAIGTDRVGIRISPAIDHTD 241
Query: 905 AMDSDPRSLGLAVIERLNKLQFELGSKLSYLHVTQPRYTA--YGQTEAGTHGSEEEVAEL 1078
AMDSDPRSLGLAVIERLNKLQF+LGS+L+YLHVTQPRYTA +GQTEAG +GSEEEVA+L
Sbjct: 242 AMDSDPRSLGLAVIERLNKLQFKLGSRLAYLHVTQPRYTADGHGQTEAGANGSEEEVAQL 301
Query: 1079 MKTWRHAYVGTFICSGGYTRELGLEAVAKEDADLVSYGRLFISNPDLPLRLKLNAPLNRY 1258
MKTWR AYVGTFIC GGYTRELGL+AVA+ DADLV++GR F+SNPDL LRLKLNAPLNRY
Sbjct: 302 MKTWRGAYVGTFICCGGYTRELGLQAVAQGDADLVAFGRYFVSNPDLVLRLKLNAPLNRY 361
Query: 1259 VRATFYTHDPVVGYTDYPSLDKGN 1330
RATFYTHDPVVGYTDYPSLDKG+
Sbjct: 362 DRATFYTHDPVVGYTDYPSLDKGS 385
>gb|AER51971.1| 12-oxophytodienoate reductase 3 [Solanum tuberosum]
Length = 400
Score = 632 bits (1631), Expect = e-179
Identities = 314/401 (78%), Positives = 346/401 (86%), Gaps = 4/401 (0%)
Frame = +2
Query: 164 MAQTTPSAHKP---TLFSPYKMGNFNLSHRVVLAPMTRCRALNNTPNEALVEYYRQRATD 334
MA TT S+ + LFSPYKMG FNLSHR+VLAPMTRCRALNN P AL EYY QRAT
Sbjct: 1 MANTTSSSAQDGNNPLFSPYKMGKFNLSHRIVLAPMTRCRALNNIPQAALGEYYEQRATA 60
Query: 335 GGFLITEGTMISPTSAGFPHVPGIFNKEQVEGWKKVVDAVHKEGAVIFCQLWHVGRASHQ 514
GGFLITEGTMISPTSAGFPHVPGIF KEQVE WKK+VD VH +GAVIFCQLWHVGRASH+
Sbjct: 61 GGFLITEGTMISPTSAGFPHVPGIFTKEQVEEWKKIVDVVHAKGAVIFCQLWHVGRASHE 120
Query: 515 VYQPGGAAPISSTNKPISKKWRILMADATYNRYPEPRPLAIHEIPEVVEDYRLAAINAIE 694
VYQP GAAPISST PISK+WRILM D T+ YP+PR + +EI +VVEDYR +A+NAIE
Sbjct: 121 VYQPAGAAPISSTEMPISKRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIE 180
Query: 695 AGFDGIEIHGAHGYLLDQFMKDGINDRTDEYGGSLANRCKFLLQVVKAVATAIGTDRVGV 874
AGFDGIEIHGAHGYL+DQF+KDGINDRTDEYGGSLANRCKF+ QVV+AV +AIG DRVGV
Sbjct: 181 AGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGV 240
Query: 875 RISPAIDHLDAMDSDPRSLGLAVIERLNKLQFELGSKLSYLHVTQPRYTAYGQTEAGTHG 1054
R+SPAIDHLDAMDS+P SLGLAV+ERLNK+Q GSKL+YLHVTQPRY AYGQTEAG G
Sbjct: 241 RVSPAIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLG 300
Query: 1055 SEEEVAELMKTWRHAYVGTFICSGGYTRELGLEAVAKEDADLVSYGRLFISNPDLPLRLK 1234
SEEE A LM+T R+AY GTFICSGGYTRELG+EAVA+ DADLVSYGRLFISNPDL +R+K
Sbjct: 301 SEEEEARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIK 360
Query: 1235 LNAPLNRYVRATFYTHDPVVGYTDYPSLDKGNASFGP-SRL 1354
LNAPLN+Y R TFYT DPVVGYTDYP L +GN S GP SRL
Sbjct: 361 LNAPLNKYNRKTFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 400
>ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lycopersicum]
gi|62900706|sp|Q9FEW9.1|OPR3_SOLLC RecName:
Full=12-oxophytodienoate reductase 3; AltName:
Full=12-oxophytodienoate-10,11-reductase 3;
Short=OPDA-reductase 3; AltName: Full=LeOPR3
gi|12056507|emb|CAC21424.1| 12-oxophytodienoate reductase
3 [Solanum lycopersicum]
Length = 396
Score = 631 bits (1627), Expect = e-178
Identities = 312/398 (78%), Positives = 343/398 (86%), Gaps = 1/398 (0%)
Frame = +2
Query: 164 MAQTTPSAHKPTLFSPYKMGNFNLSHRVVLAPMTRCRALNNTPNEALVEYYRQRATDGGF 343
MA + + P LFSPYKMG FNLSHRVVLAPMTRCRALNN P AL EYY QRAT GGF
Sbjct: 1 MASSAQDGNNP-LFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGF 59
Query: 344 LITEGTMISPTSAGFPHVPGIFNKEQVEGWKKVVDAVHKEGAVIFCQLWHVGRASHQVYQ 523
LITEGTMISPTSAGFPHVPGIF KEQV WKK+VD VH +GAVIFCQLWHVGRASH+VYQ
Sbjct: 60 LITEGTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQ 119
Query: 524 PGGAAPISSTNKPISKKWRILMADATYNRYPEPRPLAIHEIPEVVEDYRLAAINAIEAGF 703
P GAAPISST KPIS +WRILM D T+ YP+PR + +EI +VVEDYR +A+NAIEAGF
Sbjct: 120 PAGAAPISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGF 179
Query: 704 DGIEIHGAHGYLLDQFMKDGINDRTDEYGGSLANRCKFLLQVVKAVATAIGTDRVGVRIS 883
DGIEIHGAHGYL+DQF+KDGINDRTDEYGGSLANRCKF+ QVV+AV +AIG DRVGVR+S
Sbjct: 180 DGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVS 239
Query: 884 PAIDHLDAMDSDPRSLGLAVIERLNKLQFELGSKLSYLHVTQPRYTAYGQTEAGTHGSEE 1063
PAIDHLDAMDS+P SLGLAV+ERLNK+Q GSKL+YLHVTQPRY AYGQTEAG GSEE
Sbjct: 240 PAIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEE 299
Query: 1064 EVAELMKTWRHAYVGTFICSGGYTRELGLEAVAKEDADLVSYGRLFISNPDLPLRLKLNA 1243
E A LM+T R+AY GTFICSGGYTRELG+EAVA+ DADLVSYGRLFISNPDL +R+KLNA
Sbjct: 300 EEARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNA 359
Query: 1244 PLNRYVRATFYTHDPVVGYTDYPSLDKGNASFGP-SRL 1354
PLN+Y R TFYT DPVVGYTDYP L +GN S GP SRL
Sbjct: 360 PLNKYNRKTFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 396
>pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-
Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|116667815|pdb|2HS6|B Chain B, Crystal Structure Of The
E291k Mutant Of 12- Oxophytodienoate Reductase 3 (Opr3)
From Tomato gi|116667818|pdb|2HSA|B Chain B, Crystal
Structure Of 12-Oxophytodienoate Reductase 3 (Opr3) From
Tomato gi|116667819|pdb|2HSA|A Chain A, Crystal Structure
Of 12-Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|256599757|pdb|3HGS|A Chain A, Crystal Structure Of
Tomato Opr3 In Complex With Phb gi|256599758|pdb|3HGS|B
Chain B, Crystal Structure Of Tomato Opr3 In Complex With
Phb
Length = 402
Score = 631 bits (1627), Expect = e-178
Identities = 312/398 (78%), Positives = 343/398 (86%), Gaps = 1/398 (0%)
Frame = +2
Query: 164 MAQTTPSAHKPTLFSPYKMGNFNLSHRVVLAPMTRCRALNNTPNEALVEYYRQRATDGGF 343
MA + + P LFSPYKMG FNLSHRVVLAPMTRCRALNN P AL EYY QRAT GGF
Sbjct: 7 MASSAQDGNNP-LFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGF 65
Query: 344 LITEGTMISPTSAGFPHVPGIFNKEQVEGWKKVVDAVHKEGAVIFCQLWHVGRASHQVYQ 523
LITEGTMISPTSAGFPHVPGIF KEQV WKK+VD VH +GAVIFCQLWHVGRASH+VYQ
Sbjct: 66 LITEGTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQ 125
Query: 524 PGGAAPISSTNKPISKKWRILMADATYNRYPEPRPLAIHEIPEVVEDYRLAAINAIEAGF 703
P GAAPISST KPIS +WRILM D T+ YP+PR + +EI +VVEDYR +A+NAIEAGF
Sbjct: 126 PAGAAPISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGF 185
Query: 704 DGIEIHGAHGYLLDQFMKDGINDRTDEYGGSLANRCKFLLQVVKAVATAIGTDRVGVRIS 883
DGIEIHGAHGYL+DQF+KDGINDRTDEYGGSLANRCKF+ QVV+AV +AIG DRVGVR+S
Sbjct: 186 DGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVS 245
Query: 884 PAIDHLDAMDSDPRSLGLAVIERLNKLQFELGSKLSYLHVTQPRYTAYGQTEAGTHGSEE 1063
PAIDHLDAMDS+P SLGLAV+ERLNK+Q GSKL+YLHVTQPRY AYGQTEAG GSEE
Sbjct: 246 PAIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEE 305
Query: 1064 EVAELMKTWRHAYVGTFICSGGYTRELGLEAVAKEDADLVSYGRLFISNPDLPLRLKLNA 1243
E A LM+T R+AY GTFICSGGYTRELG+EAVA+ DADLVSYGRLFISNPDL +R+KLNA
Sbjct: 306 EEARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNA 365
Query: 1244 PLNRYVRATFYTHDPVVGYTDYPSLDKGNASFGP-SRL 1354
PLN+Y R TFYT DPVVGYTDYP L +GN S GP SRL
Sbjct: 366 PLNKYNRKTFYTQDPVVGYTDYPFL-QGNGSNGPLSRL 402