BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00005105
         (1819 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    866   0.0  
gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        844   0.0  
sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desatur...   828   0.0  
ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vi...   721   0.0  
ref|XP_004138473.1| PREDICTED: delta(8)-fatty-acid desaturase-li...   700   0.0  

>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  866 bits (2238), Expect = 0.0
 Identities = 410/467 (87%), Positives = 429/467 (91%)
 Frame = +2

Query: 179  MCEDSAMTSPSIAVVEVLNSKADGKKYITSEELQKHTKPNDLWISIQGKVYNVTEWAKIH 358
            MCEDS M S  + V++  NSK+DGKKYITS+EL+KH KPNDLWISIQGKVYNVTEWAKIH
Sbjct: 1    MCEDSTMVSSPVDVLKS-NSKSDGKKYITSDELKKHNKPNDLWISIQGKVYNVTEWAKIH 59

Query: 359  PGGDIPLMNLAGQDVTDAFIAFHPGSAWQHLEKLFTGYHLKDYQVSDVSKDYRKLASEFA 538
            PGG+IPLMNLAGQDVTDAFIAFHPGSAW+HL KLFTGYHLKDYQVS VSKDYRKLASEFA
Sbjct: 60   PGGEIPLMNLAGQDVTDAFIAFHPGSAWEHLSKLFTGYHLKDYQVSAVSKDYRKLASEFA 119

Query: 539  KAGMFEKKGHGVIYXXXXXXXXXXXXXYGVLYSSSFWVHIXXXXXXXXXWIQIAYLGHDA 718
            KAGMFEKKGHGVIY             YGVL+SSSFWVH+         WIQIAYLGHDA
Sbjct: 120  KAGMFEKKGHGVIYSLCFVSLLLSGCVYGVLFSSSFWVHMLAGALLGLAWIQIAYLGHDA 179

Query: 719  GHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS 898
            GHYQMM+TRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS
Sbjct: 180  GHYQMMSTRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS 239

Query: 899  SKLFNSLTSVFYGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLCSRRKVPD 1078
            S+LFNSLTSVFYGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLL S+RKVPD
Sbjct: 240  SRLFNSLTSVFYGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLLSKRKVPD 299

Query: 1079 RGLNILGTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGAP 1258
            +GLNI GTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVG P
Sbjct: 300  KGLNIFGTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGPP 359

Query: 1259 KGNDWFEKQTSGTIDISCSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKKH 1438
             GNDWFEKQT+GTIDI+CSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSI+PIVRELCKKH
Sbjct: 360  TGNDWFEKQTNGTIDIACSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSIAPIVRELCKKH 419

Query: 1439 NLPYTSLSFFDANIATLKTLKTAALQARDLTNLAPQNLLWEAFNTHG 1579
            NLPYTS+SFFDANI TLKTL+TAALQARDLTN APQNLLWEAFNTHG
Sbjct: 420  NLPYTSVSFFDANIMTLKTLRTAALQARDLTNPAPQNLLWEAFNTHG 466


>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  844 bits (2180), Expect = 0.0
 Identities = 393/469 (83%), Positives = 424/469 (90%), Gaps = 2/469 (0%)
 Frame = +2

Query: 179  MCEDSAMTSPSIAVVEVLNS--KADGKKYITSEELQKHTKPNDLWISIQGKVYNVTEWAK 352
            MCEDS M SPS+AVVEVL S   +DGKKYITSEEL+KH KP DLWISIQGKVYNVTEW+K
Sbjct: 1    MCEDSTMVSPSVAVVEVLKSTSSSDGKKYITSEELEKHNKPTDLWISIQGKVYNVTEWSK 60

Query: 353  IHPGGDIPLMNLAGQDVTDAFIAFHPGSAWQHLEKLFTGYHLKDYQVSDVSKDYRKLASE 532
            IHPGGDIPL+NLAGQDVTDAFIAFHPGSAW HL+ LFTGYHL+DYQVSD+SKDYRKLASE
Sbjct: 61   IHPGGDIPLLNLAGQDVTDAFIAFHPGSAWHHLQSLFTGYHLQDYQVSDISKDYRKLASE 120

Query: 533  FAKAGMFEKKGHGVIYXXXXXXXXXXXXXYGVLYSSSFWVHIXXXXXXXXXWIQIAYLGH 712
            F+KAGMF+KKGHGVIY             YGVL S+S  VH+         W+QIAYLGH
Sbjct: 121  FSKAGMFDKKGHGVIYSLCFVSILLSLCVYGVLCSASVTVHMFAGALLGLAWMQIAYLGH 180

Query: 713  DAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLA 892
            DAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLA
Sbjct: 181  DAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLA 240

Query: 893  VSSKLFNSLTSVFYGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLCSRRKV 1072
            VSSKLF S+TSVFYGR+LTFD +ARFF+SYQHY+YYPIMCV RVNLYLQT+LLLCS+R++
Sbjct: 241  VSSKLFESITSVFYGRRLTFDSVARFFISYQHYTYYPIMCVTRVNLYLQTILLLCSKRRI 300

Query: 1073 PDRGLNILGTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVG 1252
            PDR LNI+GTL+FWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAA+VYVG
Sbjct: 301  PDRTLNIIGTLIFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVG 360

Query: 1253 APKGNDWFEKQTSGTIDISCSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCK 1432
             P+GNDWFEKQT+GTIDISCSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIV +LCK
Sbjct: 361  PPRGNDWFEKQTAGTIDISCSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVMDLCK 420

Query: 1433 KHNLPYTSLSFFDANIATLKTLKTAALQARDLTNLAPQNLLWEAFNTHG 1579
            KHNLPYTSLSFFDAN+ TLKTL+TAALQARDL N  PQNL+WEAFNTHG
Sbjct: 421  KHNLPYTSLSFFDANVTTLKTLRTAALQARDLANGPPQNLVWEAFNTHG 469


>sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
            Full=Delta(8)-sphingolipid desaturase
            gi|1040729|emb|CAA60621.1| delta-8 sphingolipid
            desaturase [Helianthus annuus]
          Length = 458

 Score =  828 bits (2140), Expect = 0.0
 Identities = 389/461 (84%), Positives = 416/461 (90%)
 Frame = +2

Query: 197  MTSPSIAVVEVLNSKADGKKYITSEELQKHTKPNDLWISIQGKVYNVTEWAKIHPGGDIP 376
            M SPSI   EVLNS ADGKKYITS+EL+KH  PNDLWISI GKVYNVTEWAK HPGGD P
Sbjct: 1    MVSPSI---EVLNSIADGKKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAP 57

Query: 377  LMNLAGQDVTDAFIAFHPGSAWQHLEKLFTGYHLKDYQVSDVSKDYRKLASEFAKAGMFE 556
            L+NLAGQDVTDAFIAFHPG+AW+HL+KLFTGYHLKDYQVSD+S+DYRKLASEFAKAGMFE
Sbjct: 58   LINLAGQDVTDAFIAFHPGTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFE 117

Query: 557  KKGHGVIYXXXXXXXXXXXXXYGVLYSSSFWVHIXXXXXXXXXWIQIAYLGHDAGHYQMM 736
            KKGHGVIY             YGVLYS SFW+H+         W+QIAYLGHDAGHYQMM
Sbjct: 118  KKGHGVIYSLCFVSLLLSACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMM 177

Query: 737  ATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNS 916
            ATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNS
Sbjct: 178  ATRGWNKFAGIFIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNS 237

Query: 917  LTSVFYGRQLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLCSRRKVPDRGLNIL 1096
            +TSVFYGRQLTFD LARFFVSYQHY YYPIMCVARVNLYLQT+LLL S+RK+PDRGLNIL
Sbjct: 238  ITSVFYGRQLTFDPLARFFVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNIL 297

Query: 1097 GTLVFWTWFPLLVSCLPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGAPKGNDWF 1276
            GTL+FWTWFPLLVS LPNWPERVAFVLVSFCVTGIQH+QFTLNHF+ +VYVG PKG++WF
Sbjct: 298  GTLIFWTWFPLLVSRLPNWPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWF 357

Query: 1277 EKQTSGTIDISCSSYMDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKKHNLPYTS 1456
            EKQT GTIDI+CSS+MDWFFGGLQFQLEHHLFPRLPRCHLRSISPI RELCKK+NLPY S
Sbjct: 358  EKQTRGTIDIACSSWMDWFFGGLQFQLEHHLFPRLPRCHLRSISPICRELCKKYNLPYVS 417

Query: 1457 LSFFDANIATLKTLKTAALQARDLTNLAPQNLLWEAFNTHG 1579
            LSF+DAN+ TLKTL+TAALQARDLTN APQNL WEAFNTHG
Sbjct: 418  LSFYDANVTTLKTLRTAALQARDLTNPAPQNLAWEAFNTHG 458


>ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vinifera]
          Length = 447

 Score =  721 bits (1861), Expect = 0.0
 Identities = 325/446 (72%), Positives = 385/446 (86%)
 Frame = +2

Query: 242  ADGKKYITSEELQKHTKPNDLWISIQGKVYNVTEWAKIHPGGDIPLMNLAGQDVTDAFIA 421
            A  +KYI++EEL++H KP DLWISIQGKVYNVT+WAK+HPGGDIPL+NLAGQDVTDAFIA
Sbjct: 2    AGERKYISAEELKQHDKPGDLWISIQGKVYNVTDWAKVHPGGDIPLLNLAGQDVTDAFIA 61

Query: 422  FHPGSAWQHLEKLFTGYHLKDYQVSDVSKDYRKLASEFAKAGMFEKKGHGVIYXXXXXXX 601
            +HPG+AWQ+L+K FTGY+LKD++VS+VSKD+RKLA+EF+K G+FEKKGHG++Y       
Sbjct: 62   YHPGTAWQYLDKFFTGYYLKDFKVSEVSKDFRKLANEFSKLGLFEKKGHGILYTCSIIGF 121

Query: 602  XXXXXXYGVLYSSSFWVHIXXXXXXXXXWIQIAYLGHDAGHYQMMATRGWNKFAGIFIGN 781
                  YGVL   SFWVH+         WIQ +YLGHD+GHYQ+M++ G+NK A + IGN
Sbjct: 122  LFMVCIYGVLCVDSFWVHLGCAALLGFLWIQSSYLGHDSGHYQIMSSPGFNKLAQMLIGN 181

Query: 782  CITGISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSLTSVFYGRQLTFDRL 961
            CITGISIAWWKWTHNAHHIACNSLDYDPDLQH+P+ AVSS LF+S+ SVFYGR L FD +
Sbjct: 182  CITGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAVSSSLFDSINSVFYGRILKFDSV 241

Query: 962  ARFFVSYQHYSYYPIMCVARVNLYLQTLLLLCSRRKVPDRGLNILGTLVFWTWFPLLVSC 1141
            ARFFVSYQH++YYP+MCVAR+NL+LQTLLLL S R +PDR LNI+G  VFWTW+PLLVSC
Sbjct: 242  ARFFVSYQHWTYYPVMCVARINLFLQTLLLLFSGRNIPDRALNIMGICVFWTWYPLLVSC 301

Query: 1142 LPNWPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGAPKGNDWFEKQTSGTIDISCSSY 1321
            LPNW ERV FV+VS  VT IQH+QFTLNHF+ANVYVG P GNDWFEKQTSGTIDISCS++
Sbjct: 302  LPNWGERVMFVIVSMAVTSIQHIQFTLNHFSANVYVGPPCGNDWFEKQTSGTIDISCSAW 361

Query: 1322 MDWFFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKKHNLPYTSLSFFDANIATLKTLK 1501
            MDWF GGLQFQLEHHLFPRLPRCHLRSISP+V++LCKKHNLPY SLSF++AN++T++TL+
Sbjct: 362  MDWFHGGLQFQLEHHLFPRLPRCHLRSISPVVKDLCKKHNLPYRSLSFWEANVSTIRTLR 421

Query: 1502 TAALQARDLTNLAPQNLLWEAFNTHG 1579
            TAALQARDLTN  P+NL+WEA N HG
Sbjct: 422  TAALQARDLTNPVPKNLVWEAANIHG 447


>ref|XP_004138473.1| PREDICTED: delta(8)-fatty-acid desaturase-like isoform 1 [Cucumis
            sativus] gi|449441408|ref|XP_004138474.1| PREDICTED:
            delta(8)-fatty-acid desaturase-like isoform 2 [Cucumis
            sativus] gi|449495238|ref|XP_004159774.1| PREDICTED:
            delta(8)-fatty-acid desaturase-like isoform 1 [Cucumis
            sativus] gi|449495242|ref|XP_004159775.1| PREDICTED:
            delta(8)-fatty-acid desaturase-like isoform 2 [Cucumis
            sativus]
          Length = 447

 Score =  700 bits (1807), Expect = 0.0
 Identities = 322/443 (72%), Positives = 370/443 (83%)
 Frame = +2

Query: 251  KKYITSEELQKHTKPNDLWISIQGKVYNVTEWAKIHPGGDIPLMNLAGQDVTDAFIAFHP 430
            KKYITSEEL KH KP DLWISIQGKVYNVTEWAK HPGGD P +NLAG DVTDAFIA+HP
Sbjct: 5    KKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFIAYHP 64

Query: 431  GSAWQHLEKLFTGYHLKDYQVSDVSKDYRKLASEFAKAGMFEKKGHGVIYXXXXXXXXXX 610
            G+AW +L++LFTG+HLKD+QVS++SKDYRKLASEF+K G+FEKKGH  +Y          
Sbjct: 65   GTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVAVMMF 124

Query: 611  XXXYGVLYSSSFWVHIXXXXXXXXXWIQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 790
               YGVL S S  VH+         WIQ AY+GHD+GH  +M++RG+NK A I  GNC+T
Sbjct: 125  LVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 184

Query: 791  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSLTSVFYGRQLTFDRLARF 970
            GISIAWWKWTHNAHHI+CNSLD+DPDLQH+P+ AVS+  F +LTS FYGR+L FD LARF
Sbjct: 185  GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDSLARF 244

Query: 971  FVSYQHYSYYPIMCVARVNLYLQTLLLLCSRRKVPDRGLNILGTLVFWTWFPLLVSCLPN 1150
            FVSYQH+++YP+MCVARVNL++QTLLLL S RKVPDR LNI+G LVFWTWFPLLVS LPN
Sbjct: 245  FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 304

Query: 1151 WPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGAPKGNDWFEKQTSGTIDISCSSYMDW 1330
            WPERV FVL SF V  +QH+QF LNHF+ANVYVG P GNDWFEKQ SGT+DISCS++MDW
Sbjct: 305  WPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCSTWMDW 364

Query: 1331 FFGGLQFQLEHHLFPRLPRCHLRSISPIVRELCKKHNLPYTSLSFFDANIATLKTLKTAA 1510
            FFGGLQFQLEHHLFPR+PRC LR ISPIV ELCKKHNLPY SLSF+DAN+ T++TL+TAA
Sbjct: 365  FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 424

Query: 1511 LQARDLTNLAPQNLLWEAFNTHG 1579
            LQARDLTN  P+NLLWEA NTHG
Sbjct: 425  LQARDLTNPVPKNLLWEAVNTHG 447