BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00003434
         (1677 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q04468.1|TCMO_HELTU RecName: Full=Trans-cinnamate 4-monooxyge...   883   0.0  
gb|ADO16181.1| cytochrome P450 mono-oxygenase [Artemisia annua]       882   0.0  
gb|ACF74449.1| trans-cinnamate 4-monooxygenase [Echinacea angust...   879   0.0  
gb|ACJ37399.1| trans-cinnamate 4-monooxygenase [Echinacea angust...   864   0.0  
dbj|BAJ17666.1| cinnamate 4-hydroxylase [Gynura bicolor]              862   0.0  

>sp|Q04468.1|TCMO_HELTU RecName: Full=Trans-cinnamate 4-monooxygenase; AltName: Full=Cinnamic
            acid 4-hydroxylase; Short=C4H; Short=CA4H; AltName:
            Full=Cytochrome P450 73; AltName: Full=Cytochrome P450C4H
            gi|18859|emb|CAA78982.1| trans-cinnamate 4-monooxygenase
            [Helianthus tuberosus]
          Length = 505

 Score =  883 bits (2282), Expect = 0.0
 Identities = 442/505 (87%), Positives = 454/505 (89%)
 Frame = -2

Query: 1637 MDLLLLEKTXXXXXXXXXXXXXISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 1458
            MDLLL+EKT             ISKLRGK+FKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLIEKTLVALFAAIIGAILISKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 1457 LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 1278
            LAK+FGEI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKRFGEILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 1277 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXVKKNPASATEGIVIRRRLQL 1098
            VYGEHWRKMRRIMTVPFFTNKVVQQYR+GW          VKKNPA+ATEGIVIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEAEAAAVVDDVKKNPAAATEGIVIRRRLQL 180

Query: 1097 MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 918
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLR YLK
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRNYLK 240

Query: 917  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 738
            LCKEVK+KR QLFKDYFVDERKK+GSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV
Sbjct: 241  LCKEVKDKRIQLFKDYFVDERKKIGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 300

Query: 737  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 558
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 360

Query: 557  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 378
            +KETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNP+QWKK        
Sbjct: 361  VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPER 420

Query: 377  XXXEDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFELLPPPGQSKID 198
               E+AKVEANGNDFRYLPFGVGRRSCP             GRLVQNFELLPPPGQSKID
Sbjct: 421  FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKID 480

Query: 197  TSEKGGQFSLHILKHSTVVAKPRSF 123
            T EKGGQFSLHILKHST+VAKPRSF
Sbjct: 481  TDEKGGQFSLHILKHSTIVAKPRSF 505


>gb|ADO16181.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 505

 Score =  882 bits (2278), Expect = 0.0
 Identities = 441/505 (87%), Positives = 453/505 (89%)
 Frame = -2

Query: 1637 MDLLLLEKTXXXXXXXXXXXXXISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 1458
            MDLLLLEKT             ISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLLEKTLLGLLVAILGAIFISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 1457 LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 1278
            LAKKFGEIFLLRMGQRNLVVVSSPDLAK+VLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKKFGEIFLLRMGQRNLVVVSSPDLAKDVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 1277 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXVKKNPASATEGIVIRRRLQL 1098
            VYGEHWRKMRRIMTVPFFTNKVVQQYR GW          VKKNPASATEG V+RRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWEAEAAAVVEDVKKNPASATEGTVLRRRLQL 180

Query: 1097 MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 918
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 917  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 738
            LCKEVK+KR QLFKDYFVDERKKLGSTK +DNNQ+KCAIDHILEAK+KGEINEDNVLYIV
Sbjct: 241  LCKEVKDKRLQLFKDYFVDERKKLGSTKSLDNNQIKCAIDHILEAKDKGEINEDNVLYIV 300

Query: 737  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 558
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQ+TEPD+QNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQVTEPDIQNLPYLQAV 360

Query: 557  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 378
            IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNP+QWKK        
Sbjct: 361  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPER 420

Query: 377  XXXEDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFELLPPPGQSKID 198
               E++KVEANGNDFRYLPFGVGRRSCP             GRLVQNFELLPPPG SKID
Sbjct: 421  FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGVSKID 480

Query: 197  TSEKGGQFSLHILKHSTVVAKPRSF 123
            TSEKGGQFSLHILKHST+VAKPRSF
Sbjct: 481  TSEKGGQFSLHILKHSTIVAKPRSF 505


>gb|ACF74449.1| trans-cinnamate 4-monooxygenase [Echinacea angustifolia]
          Length = 505

 Score =  879 bits (2270), Expect = 0.0
 Identities = 439/505 (86%), Positives = 455/505 (90%)
 Frame = -2

Query: 1637 MDLLLLEKTXXXXXXXXXXXXXISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 1458
            MDL L+EKT             +SKLRGK+FKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLPLIEKTLIALFAAIIGAIVVSKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 1457 LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 1278
            LAKKFG+IFLLRMGQRNLVVVSSP+LAK+VLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKKFGQIFLLRMGQRNLVVVSSPELAKDVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 1277 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXVKKNPASATEGIVIRRRLQL 1098
            VYGEHWRKMRRIMTVPFFTNKVVQQYR+GW          VKKNPA+ATEG+VIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEAEAAAVVEDVKKNPAAATEGVVIRRRLQL 180

Query: 1097 MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 918
            MMYNNMFRIMFDRRFESE+DPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLR YLK
Sbjct: 181  MMYNNMFRIMFDRRFESEEDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRNYLK 240

Query: 917  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 738
            LCKEVKEKR QLFKDYFVDERKKLGSTKKMD+NQLKCAIDHILEAK+KGEINEDNVLYIV
Sbjct: 241  LCKEVKEKRIQLFKDYFVDERKKLGSTKKMDDNQLKCAIDHILEAKDKGEINEDNVLYIV 300

Query: 737  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 558
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 360

Query: 557  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 378
            IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNP+QWKK        
Sbjct: 361  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPER 420

Query: 377  XXXEDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFELLPPPGQSKID 198
               E+AKV+ANGNDFRYLPFGVGRRSCP             GRLVQNFELLPPPGQSKID
Sbjct: 421  FLEEEAKVDANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKID 480

Query: 197  TSEKGGQFSLHILKHSTVVAKPRSF 123
            T+EKGGQFSLHILKHSTVVAKPRSF
Sbjct: 481  TAEKGGQFSLHILKHSTVVAKPRSF 505


>gb|ACJ37399.1| trans-cinnamate 4-monooxygenase [Echinacea angustifolia]
          Length = 505

 Score =  864 bits (2232), Expect = 0.0
 Identities = 430/503 (85%), Positives = 448/503 (89%)
 Frame = -2

Query: 1637 MDLLLLEKTXXXXXXXXXXXXXISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 1458
            MDLLLLEKT             ISKLRGKRFKLPPGP+PVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLLEKTLIALFAAIIASIFISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 1457 LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 1278
            LAKKFGEI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKKFGEILLLRMGQRNLVVVSSPNLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 1277 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXVKKNPASATEGIVIRRRLQL 1098
            VYGEHWRKMRRIMTVPFFTNKVVQQY  GW          V+KNP +ATEG+VIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYHSGWEAEAAAVVEDVRKNPKAATEGVVIRRRLQL 180

Query: 1097 MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 918
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFL+GYLK
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 917  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 738
            LCKEVKEKRFQLFKDYFVDERKKLGSTK MDNNQLKCAIDHIL+AK+KGEINEDNVLYIV
Sbjct: 241  LCKEVKEKRFQLFKDYFVDERKKLGSTKSMDNNQLKCAIDHILDAKDKGEINEDNVLYIV 300

Query: 737  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 558
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQ+TEPD+  LPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQVTEPDLDKLPYLQAV 360

Query: 557  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 378
            IKETLRLRMAIPLLVPHMNLHDAKLGG+DIPAESKILVNAWWLANNP+QWK+        
Sbjct: 361  IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDQWKEPEVFRPER 420

Query: 377  XXXEDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFELLPPPGQSKID 198
               E++KVEANGNDFRYLPFGVGRRSCP             GRLVQNFELLPPPGQSK+ 
Sbjct: 421  FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKVG 480

Query: 197  TSEKGGQFSLHILKHSTVVAKPR 129
            T+EKGGQFSLHILKHST+VAKPR
Sbjct: 481  TTEKGGQFSLHILKHSTIVAKPR 503


>dbj|BAJ17666.1| cinnamate 4-hydroxylase [Gynura bicolor]
          Length = 505

 Score =  862 bits (2227), Expect = 0.0
 Identities = 430/505 (85%), Positives = 447/505 (88%)
 Frame = -2

Query: 1637 MDLLLLEKTXXXXXXXXXXXXXISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 1458
            MDLLLLEKT             ISKLRGK+FKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLLEKTLIALFTAIIIAIVISKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 1457 LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 1278
            +AKKFG+IF LRMGQRNL+VVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   MAKKFGDIFHLRMGQRNLIVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 1277 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXVKKNPASATEGIVIRRRLQL 1098
            VYGEHWRKMRRIMTVPFFTNKVVQQYR+GW          VKKNPA+ATEG+VIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAAVVEDVKKNPAAATEGVVIRRRLQL 180

Query: 1097 MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 918
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLR YL 
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRKYLN 240

Query: 917  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 738
            LCKEVK+KR QLFKDYFVDERKK+GSTKKMD N+LKCAIDHILEAKEKGEINEDNVLYIV
Sbjct: 241  LCKEVKDKRIQLFKDYFVDERKKIGSTKKMDKNELKCAIDHILEAKEKGEINEDNVLYIV 300

Query: 737  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 558
            ENINVAAIETTLWSIEWG+AELVNHP+IQAKLRHELDTKLGP VQITEPDVQNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGLAELVNHPKIQAKLRHELDTKLGPSVQITEPDVQNLPYLQAV 360

Query: 557  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 378
            +KETLRLRMAIPLLVPHMNLHDAKLGG+DIPAESKILVNAWWLANNP+ WK         
Sbjct: 361  VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDHWKNANEFRPER 420

Query: 377  XXXEDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFELLPPPGQSKID 198
               E+AKVEANGNDFRYLPFGVGRRSCP             GRLVQNFELLPPPGQ KID
Sbjct: 421  FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILAITLGRLVQNFELLPPPGQDKID 480

Query: 197  TSEKGGQFSLHILKHSTVVAKPRSF 123
             SEKGGQFSLHILKH TVVAKPRSF
Sbjct: 481  MSEKGGQFSLHILKHYTVVAKPRSF 505