BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00003210
         (1109 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      217   5e-54
gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   217   5e-54
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   181   4e-43
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   145   2e-32
ref|XP_002299099.1| cytochrome P450 [Populus trichocarpa] gi|222...   124   5e-26

>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  217 bits (552), Expect = 5e-54
 Identities = 113/190 (59%), Positives = 128/190 (67%), Gaps = 1/190 (0%)
 Frame = -2

Query: 568 MIQFLTPIFXXXXXXXXXV-YRNRKSGSAKINLPPGSFGWPFXXXXXXXXXXXXXXXXXX 392
           MIQ LTPI            Y+++K+   KINLPPGSFGWPF                  
Sbjct: 1   MIQVLTPILLFLIFFVFWKVYKHQKT---KINLPPGSFGWPFLGETLALLRAGWDSEPER 57

Query: 391 XXXXXXXXXXXXXXXXXXXERIEKHGSPLVFKTSLIGERMAVLCGPAGNKFLFGNENRLV 212
                              ERI+KHGSPLVFKTSL G+R AVLCGPAGNKFLF NEN+LV
Sbjct: 58  FVR----------------ERIKKHGSPLVFKTSLFGDRFAVLCGPAGNKFLFCNENKLV 101

Query: 211 AVWWPLTMRKLFGRCLLTIRGDEAKWMRKMVLSYLGPDAFATHYAATMDSVTRRHIQIHW 32
           A WWP+ +RKLFG+ LLTIRGDEAKWMRKM+LSYLGPDAFATHYA TMD VTRRHI +HW
Sbjct: 102 ASWWPVPVRKLFGKSLLTIRGDEAKWMRKMLLSYLGPDAFATHYAVTMDVVTRRHIDVHW 161

Query: 31  QGKEEVNVYK 2
           +GKEEVNV++
Sbjct: 162 RGKEEVNVFQ 171


>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
           gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
           13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  217 bits (552), Expect = 5e-54
 Identities = 113/190 (59%), Positives = 128/190 (67%), Gaps = 1/190 (0%)
 Frame = -2

Query: 568 MIQFLTPIFXXXXXXXXXV-YRNRKSGSAKINLPPGSFGWPFXXXXXXXXXXXXXXXXXX 392
           MIQ LTPI            Y+++K+   KINLPPGSFGWPF                  
Sbjct: 1   MIQVLTPILLFLIFFVFWKVYKHQKT---KINLPPGSFGWPFLGETLALLRAGWDSEPER 57

Query: 391 XXXXXXXXXXXXXXXXXXXERIEKHGSPLVFKTSLIGERMAVLCGPAGNKFLFGNENRLV 212
                              ERI+KHGSPLVFKTSL G+R AVLCGPAGNKFLF NEN+LV
Sbjct: 58  FVR----------------ERIKKHGSPLVFKTSLFGDRFAVLCGPAGNKFLFCNENKLV 101

Query: 211 AVWWPLTMRKLFGRCLLTIRGDEAKWMRKMVLSYLGPDAFATHYAATMDSVTRRHIQIHW 32
           A WWP+ +RKLFG+ LLTIRGDEAKWMRKM+LSYLGPDAFATHYA TMD VTRRHI +HW
Sbjct: 102 ASWWPVPVRKLFGKSLLTIRGDEAKWMRKMLLSYLGPDAFATHYAVTMDVVTRRHIDVHW 161

Query: 31  QGKEEVNVYK 2
           +GKEEVNV++
Sbjct: 162 RGKEEVNVFQ 171


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
           annua]
          Length = 480

 Score =  181 bits (458), Expect = 4e-43
 Identities = 89/168 (52%), Positives = 107/168 (63%)
 Frame = -2

Query: 505 NRKSGSAKINLPPGSFGWPFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERI 326
           +R + +   NLPPGSFGWPF                                     ERI
Sbjct: 23  HRHNSTTTKNLPPGSFGWPFIGETLAYIRSKRGGDPERFTK----------------ERI 66

Query: 325 EKHGSPLVFKTSLIGERMAVLCGPAGNKFLFGNENRLVAVWWPLTMRKLFGRCLLTIRGD 146
           EK+GS LVFKTS+ GERMAV CGP GNKFLFGNEN+LVA WWP ++R LF +CL+TIRGD
Sbjct: 67  EKYGSTLVFKTSVAGERMAVFCGPEGNKFLFGNENKLVASWWPNSVRILFEKCLITIRGD 126

Query: 145 EAKWMRKMVLSYLGPDAFATHYAATMDSVTRRHIQIHWQGKEEVNVYK 2
           EAKW+RKM+ +YLGPDA +  Y  TM+ VTR HIQ HWQGK E+ V++
Sbjct: 127 EAKWLRKMMFAYLGPDALSNRYTGTMEVVTRLHIQNHWQGKSELKVFE 174


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
           vinifera]
          Length = 473

 Score =  145 bits (365), Expect = 2e-32
 Identities = 77/158 (48%), Positives = 94/158 (59%)
 Frame = -2

Query: 478 NLPPGSFGWPFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERIEKHGSPLVF 299
           NLPPGS GWP                                      ER++K+ S  VF
Sbjct: 30  NLPPGSLGWP----------------RIGETLVFLRSGEDGEPQRFIRERMDKYDSG-VF 72

Query: 298 KTSLIGERMAVLCGPAGNKFLFGNENRLVAVWWPLTMRKLFGRCLLTIRGDEAKWMRKMV 119
           KTSL+GE M V CGPAGNKFLFGNEN+LV+VWWP ++RKLF  CL+T  GDEAK MR+M+
Sbjct: 73  KTSLLGEPMMVFCGPAGNKFLFGNENKLVSVWWPSSVRKLFRSCLVTATGDEAKRMRRML 132

Query: 118 LSYLGPDAFATHYAATMDSVTRRHIQIHWQGKEEVNVY 5
           L++L PDA    Y  TMD VT+ HI+  W+G EE+ VY
Sbjct: 133 LTFLNPDAL-KRYVETMDLVTQHHIRTQWEGNEEMQVY 169


>ref|XP_002299099.1| cytochrome P450 [Populus trichocarpa] gi|222846357|gb|EEE83904.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 334

 Score =  124 bits (310), Expect = 5e-26
 Identities = 60/108 (55%), Positives = 78/108 (72%)
 Frame = -2

Query: 328 IEKHGSPLVFKTSLIGERMAVLCGPAGNKFLFGNENRLVAVWWPLTMRKLFGRCLLTIRG 149
           +EKH  P VFKTSL GE +AV CGPAGNKFLF NEN+LV +WWP +++KL    L+ + G
Sbjct: 1   MEKH-DPQVFKTSLFGETVAVFCGPAGNKFLFSNENKLVNLWWPRSVKKLLKSSLVNVAG 59

Query: 148 DEAKWMRKMVLSYLGPDAFATHYAATMDSVTRRHIQIHWQGKEEVNVY 5
           DEAK +R+++L++L PDA    Y   MD VT+ HI   W+GKEEV V+
Sbjct: 60  DEAKRIRRILLTFLDPDAL-KRYIERMDLVTQHHISTLWEGKEEVKVH 106