BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00001661
         (1933 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFL93685.1| 4-coumarate: coenzyme A ligase [Cynara cardunculu...  1003   0.0  
dbj|BAJ17664.1| 4-coumarate:CoA ligase [Gynura bicolor]               964   0.0  
gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandif...   916   0.0  
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   908   0.0  
sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; ...   903   0.0  

>gb|AFL93685.1| 4-coumarate: coenzyme A ligase [Cynara cardunculus var. scolymus]
          Length = 539

 Score = 1003 bits (2594), Expect = 0.0
 Identities = 507/539 (94%), Positives = 520/539 (96%)
 Frame = +1

Query: 73   MEAKAETIFRSKLPDIYIPKHLSLHSYCFENISNFSGRPCLIDGATGDVYTYADVELTSR 252
            MEAK+ETIFRS+LPDIYIPKHLSLHSYCFENIS FS RPCLIDGATGDVYTYADVELTSR
Sbjct: 1    MEAKSETIFRSRLPDIYIPKHLSLHSYCFENISAFSDRPCLIDGATGDVYTYADVELTSR 60

Query: 253  KVGSALHKHGINKGDVIMILLPNSPEFVFSFLGASYLGAVSTMANPFFTSAEIIKQAKAS 432
            KVGSALHKHGINKGDVIMILLPNSPEFV+SFLGAS++GAVSTMANPFFTSAEIIKQAKAS
Sbjct: 61   KVGSALHKHGINKGDVIMILLPNSPEFVYSFLGASFIGAVSTMANPFFTSAEIIKQAKAS 120

Query: 433  GAKIIITQSAHVAKVKDFASENSIKLVCIDSAPEECLHFSELTSGNETELPEVEISSDDV 612
             AKIIITQS H AKVKDFASENSIKLVCIDSAPEECLHFSEL SG+E ELPEVEISSDDV
Sbjct: 121  AAKIIITQSEHAAKVKDFASENSIKLVCIDSAPEECLHFSELISGDEEELPEVEISSDDV 180

Query: 613  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNS 792
            VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNS
Sbjct: 181  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNS 240

Query: 793  ILLCGLRAGAAILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLSSI 972
            ILLCGLRAGAAILIMQKF IVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDL+SI
Sbjct: 241  ILLCGLRAGAAILIMQKFHIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLTSI 300

Query: 973  RTVMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGT 1152
            RTVM GAAPLGKELE+TVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGT
Sbjct: 301  RTVMFGAAPLGKELENTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGT 360

Query: 1153 VVRNAEMKIVDPDTGASLPRNQRGEICIRGDQIMKGYLNDPEATKRTIEDGWLHTGDIGY 1332
            VVRNAEMKIVDPDTG SLPRNQRGEICIRGDQIMKGYLNDPEATK TI DGWLHTGDIGY
Sbjct: 361  VVRNAEMKIVDPDTGVSLPRNQRGEICIRGDQIMKGYLNDPEATKSTIVDGWLHTGDIGY 420

Query: 1333 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIDEVAGEVPVAFV 1512
            IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPM  E AGEVPVAFV
Sbjct: 421  IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMKHEDAGEVPVAFV 480

Query: 1513 VKSNGSNVTEDEVKKFISKQVVFYKRLNRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1689
            VKSNG+ VTE +VK+FISKQVVFYK+L RVFFID +PKSPSGKILRKDLRAKLAAGVPN
Sbjct: 481  VKSNGTTVTEVDVKQFISKQVVFYKKLYRVFFIDKVPKSPSGKILRKDLRAKLAAGVPN 539


>dbj|BAJ17664.1| 4-coumarate:CoA ligase [Gynura bicolor]
          Length = 542

 Score =  964 bits (2492), Expect = 0.0
 Identities = 480/535 (89%), Positives = 512/535 (95%), Gaps = 1/535 (0%)
 Frame = +1

Query: 88   ETIFRSKLPDIYIPKHLSLHSYCFENISNFSGRPCLIDGATGDVYTYADVELTSRKVGSA 267
            E IFRSKLPDIYIPKHL LHSYCFENIS FS RPCLIDGA+G  YTYADVELTSRKV SA
Sbjct: 8    EIIFRSKLPDIYIPKHLPLHSYCFENISKFSDRPCLIDGASGQEYTYADVELTSRKVASA 67

Query: 268  LHKHGINKGDVIMILLPNSPEFVFSFLGASYLGAVSTMANPFFTSAEIIKQAKASGAKII 447
            LH+ GINKGDVIMILLPNSPEFV++F+GASYLGA+STMANPFFT+AEIIKQAKAS AKII
Sbjct: 68   LHQQGINKGDVIMILLPNSPEFVYAFIGASYLGAISTMANPFFTAAEIIKQAKASNAKII 127

Query: 448  ITQSAHVAKVKDFASENSIKLVCIDSAPEECLHFSELTSGNETELPEVEISSDDVVALPY 627
            ITQSAHV+KVK F+SENSI +VCIDSAPE CLH+SEL SG+E++LPEVEIS DDVVALPY
Sbjct: 128  ITQSAHVSKVKRFSSENSITIVCIDSAPEGCLHYSELLSGDESKLPEVEISPDDVVALPY 187

Query: 628  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNSILLCG 807
            SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVL+CVLPLFHIYSLNSILLCG
Sbjct: 188  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLICVLPLFHIYSLNSILLCG 247

Query: 808  LRAGAAILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLSSIRTVMS 987
            LRAGAAILIMQKFDIVPFL+LI+KYKVTIGPFVPPIVLTIA NE++VDKYDLSSIRTVMS
Sbjct: 248  LRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEKIVDKYDLSSIRTVMS 307

Query: 988  GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNA 1167
            GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP++IKSGACGTVVRNA
Sbjct: 308  GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYDIKSGACGTVVRNA 367

Query: 1168 EMKIVDPDTGASLPRNQRGEICIRGDQIMKGYLNDPEATKRTIE-DGWLHTGDIGYIDDD 1344
            EMKIVDPDTG SLPRNQRGEICIRGDQIMKGYLNDPE+TK TI+ DGWLHTGDIG IDDD
Sbjct: 368  EMKIVDPDTGLSLPRNQRGEICIRGDQIMKGYLNDPESTKNTIDADGWLHTGDIGLIDDD 427

Query: 1345 DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIDEVAGEVPVAFVVKSN 1524
            DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVV M+++ AGEVPVAFVVK+N
Sbjct: 428  DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVSMVNDAAGEVPVAFVVKTN 487

Query: 1525 GSNVTEDEVKKFISKQVVFYKRLNRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1689
             S+VTEDE+K+F+SKQVVFYKR+NRVFFID IPKSPSGKILRKDLRAKLAAGVPN
Sbjct: 488  DSSVTEDEIKQFVSKQVVFYKRINRVFFIDTIPKSPSGKILRKDLRAKLAAGVPN 542


>gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandiflorus]
          Length = 556

 Score =  916 bits (2367), Expect = 0.0
 Identities = 458/537 (85%), Positives = 497/537 (92%), Gaps = 1/537 (0%)
 Frame = +1

Query: 82   KAETIFRSKLPDIYIPKHLSLHSYCFENISNFSGRPCLIDGATGDVYTYADVELTSRKVG 261
            K E IFRSKLPDIYIP HL LHSYCFENIS FS RPCLI+GATG VYTYADVE+TSRKV 
Sbjct: 21   KQEIIFRSKLPDIYIPNHLPLHSYCFENISKFSERPCLIEGATGKVYTYADVEVTSRKVA 80

Query: 262  SALHKHGINKGDVIMILLPNSPEFVFSFLGASYLGAVSTMANPFFTSAEIIKQAKASGAK 441
            S+L+K GI +GD IM+LLPNS EFVF+FLGASYLGA+STMANPFFT AEIIKQ KAS AK
Sbjct: 81   SSLNKLGIGQGDTIMLLLPNSAEFVFAFLGASYLGAISTMANPFFTHAEIIKQVKASNAK 140

Query: 442  IIITQSAHVAKVKDFASENSIKLVCIDSAPEECLHFSELTSGNETELPEVEISSDDVVAL 621
            IIIT + HV KVK+FASEN +K++CID APE CLH+SELT G+E +LPEV+IS DDVVAL
Sbjct: 141  IIITLACHVDKVKEFASENDVKVICIDKAPEGCLHYSELTLGDENDLPEVKISPDDVVAL 200

Query: 622  PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNSILL 801
            PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NL++HSEDVL+CVLPLFHIYSLNSILL
Sbjct: 201  PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLICVLPLFHIYSLNSILL 260

Query: 802  CGLRAGAAILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLSSIRTV 981
            CGLR GAAILIMQKFDIVPFLELI+KYKVTIGPFVPPIVL +AK+   VDKYDL+SIRTV
Sbjct: 261  CGLRVGAAILIMQKFDIVPFLELIEKYKVTIGPFVPPIVLAMAKSSH-VDKYDLTSIRTV 319

Query: 982  MSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVR 1161
            MSGAAPLGKELEDTVR K PNAKLGQGYGMTEAGPVLAMCLAFAKEP+EIKSGACGTVVR
Sbjct: 320  MSGAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVR 379

Query: 1162 NAEMKIVDPDTGASLPRNQRGEICIRGDQIMKGYLNDPEATKRTIE-DGWLHTGDIGYID 1338
            NAEMKIVDPDTG SLPRNQRGEICIRGDQIMKGYLNDPEAT+RTI+ +GWLHTGDIGYID
Sbjct: 380  NAEMKIVDPDTGDSLPRNQRGEICIRGDQIMKGYLNDPEATERTIDKEGWLHTGDIGYID 439

Query: 1339 DDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIDEVAGEVPVAFVVK 1518
            DDDELFIVDRLKELIKYKGFQVAPAELEALLL HP+ISDAAVV MIDE AGEVPVAFVV+
Sbjct: 440  DDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVSMIDEAAGEVPVAFVVR 499

Query: 1519 SNGSNVTEDEVKKFISKQVVFYKRLNRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1689
            +NGS +TEDE+K+FISKQVVFYKR+NRVFF D IPK+PSGKILRK+LRA+LAAG+PN
Sbjct: 500  ANGSIITEDEIKQFISKQVVFYKRINRVFFTDTIPKAPSGKILRKELRARLAAGIPN 556


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  908 bits (2347), Expect = 0.0
 Identities = 452/537 (84%), Positives = 488/537 (90%), Gaps = 1/537 (0%)
 Frame = +1

Query: 82   KAETIFRSKLPDIYIPKHLSLHSYCFENISNFSGRPCLIDGATGDVYTYADVELTSRKVG 261
            + + IFRSKLPDIYIP HL LHSYCFENIS FS RPCLI+GA   +YTYADVEL SRKV 
Sbjct: 7    QVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVA 66

Query: 262  SALHKHGINKGDVIMILLPNSPEFVFSFLGASYLGAVSTMANPFFTSAEIIKQAKASGAK 441
            + LHK GI   D IMILLPNSPEFVF+F+GASYLGA+STMANP FT AE++KQAKAS AK
Sbjct: 67   AGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 126

Query: 442  IIITQSAHVAKVKDFASENSIKLVCIDSAPEECLHFSELTSGNETELPEVEISSDDVVAL 621
            II+TQ+ HV KVKD+A EN +K++CIDSAPE CLHFS LT  NE ++PEVEI  DDVVAL
Sbjct: 127  IIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVAL 186

Query: 622  PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNSILL 801
            PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNL+IHSEDV++CVLPLFHIYSLNS+LL
Sbjct: 187  PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLL 246

Query: 802  CGLRAGAAILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLSSIRTV 981
            CGLR GAAILIMQKFDIV FLELIQ+YKVTIGPFVPPIVL IAK+  +VD YDLSS+RTV
Sbjct: 247  CGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKS-PMVDDYDLSSVRTV 305

Query: 982  MSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVR 1161
            MSGAAPLGKELEDTVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVR
Sbjct: 306  MSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVR 365

Query: 1162 NAEMKIVDPDTGASLPRNQRGEICIRGDQIMKGYLNDPEATKRTIE-DGWLHTGDIGYID 1338
            NAEMKIVDP TG SLPRNQ GEICIRGDQIMKGYLNDPEAT RTI+ +GWL+TGDIGYID
Sbjct: 366  NAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYID 425

Query: 1339 DDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIDEVAGEVPVAFVVK 1518
            DDDELFIVDRLKELIKYKGFQVAPAELEALLL HP+ISDAAVVPM DE AGEVPVAFVV+
Sbjct: 426  DDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVR 485

Query: 1519 SNGSNVTEDEVKKFISKQVVFYKRLNRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN 1689
            SNGS +TEDEVK FISKQV+FYKR+ RVFF+DAIPKSPSGKILRKDLRAKLAAG+PN
Sbjct: 486  SNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542


>sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|1663722|gb|AAB18637.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 547

 Score =  903 bits (2333), Expect = 0.0
 Identities = 453/547 (82%), Positives = 491/547 (89%), Gaps = 7/547 (1%)
 Frame = +1

Query: 70   PMEAKAET------IFRSKLPDIYIPKHLSLHSYCFENISNFSGRPCLIDGATGDVYTYA 231
            PME   ET      IFRSKLPDIYIPKHL LHSYCFENIS FS RPCLI+GA   +YTYA
Sbjct: 2    PMETTTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYA 61

Query: 232  DVELTSRKVGSALHKHGINKGDVIMILLPNSPEFVFSFLGASYLGAVSTMANPFFTSAEI 411
            +VELT RKV   L+K GI + D IMILLPNSPEFVF+F+GASYLGA+STMANP FT AE+
Sbjct: 62   EVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEV 121

Query: 412  IKQAKASGAKIIITQSAHVAKVKDFASENSIKLVCIDSAPEECLHFSELTSGNETELPEV 591
            +KQAKAS AKIIITQS  V KVKD+ASEN +K++CIDSAPE CLHFSELT  +E E+PEV
Sbjct: 122  VKQAKASSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEV 181

Query: 592  EISSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLF 771
            +I  DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NL++HSEDVLMCVLPLF
Sbjct: 182  KIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLF 241

Query: 772  HIYSLNSILLCGLRAGAAILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVD 951
            HIYSLNSILLCGLR GAAILIMQKFDI PFLELIQKYKV+IGPFVPPIVL IAK+  +VD
Sbjct: 242  HIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKS-PIVD 300

Query: 952  KYDLSSIRTVMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEI 1131
             YDLSS+RTVMSGAAPLGKELED VR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+I
Sbjct: 301  SYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDI 360

Query: 1132 KSGACGTVVRNAEMKIVDPDTGASLPRNQRGEICIRGDQIMKGYLNDPEATKRTIE-DGW 1308
            KSGACGTVVRNAEMKIVDPDTG SLPRNQ GEICIRGDQIMKGYLNDPEAT RTI+ +GW
Sbjct: 361  KSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGW 420

Query: 1309 LHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIDEVA 1488
            LHTGDIG+ID+DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP+ISDAAVVPM DE A
Sbjct: 421  LHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQA 480

Query: 1489 GEVPVAFVVKSNGSNVTEDEVKKFISKQVVFYKRLNRVFFIDAIPKSPSGKILRKDLRAK 1668
            GEVPVAFVV+SNGS +TEDEVK FISKQV+FYKR+ RVFF++ +PKSPSGKILRKDLRA+
Sbjct: 481  GEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRAR 540

Query: 1669 LAAGVPN 1689
            LAAGVPN
Sbjct: 541  LAAGVPN 547