BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes21_contig00001557
         (1763 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADM26615.1| flavonoid 3'-hydroxylase [Cynara cardunculus var....   301   0.0  
gb|ACN65826.1| flavonoid 3'-hydroxylase [Echinops bannaticus]         310   0.0  
gb|ACN65825.1| flavonoid 3'-hydroxylase [Cichorium intybus]           310   0.0  
gb|ADK56126.1| putative flavonoid 3'-hydroxylase [Coreopsis gran...   300   0.0  
gb|ACO35753.1| flavonoid 3'-hydroxylase [Bidens aurea]                302   0.0  

>gb|ADM26615.1| flavonoid 3'-hydroxylase [Cynara cardunculus var. scolymus]
          Length = 512

 Score =  301 bits (771), Expect(3) = 0.0
 Identities = 150/204 (73%), Positives = 162/204 (79%)
 Frame = -1

Query: 1730 MTLLTLVLYACITALVFYAXXXXXXXXXXXXXPGPTPWPIIGNLPHLGTSPHHSLAALAT 1551
            MT+L L+LYA IT L+ Y              PGPTPWPIIGNLPHLG  PHH+LAA+AT
Sbjct: 1    MTILPLLLYASITGLLLYVLLNLRTPRSNRLPPGPTPWPIIGNLPHLGRIPHHALAAMAT 60

Query: 1550 KYGPLLHLRLGFXXXXXXXXXXXXAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1371
            KYGPL+HLRLG             AQFLK HDANFASRPPNSGAKH+AY+YQDLVFAPYG
Sbjct: 61   KYGPLMHLRLGVVDVVVAASASVAAQFLKVHDANFASRPPNSGAKHIAYDYQDLVFAPYG 120

Query: 1370 PKWRMLRKICAVHLFSTKALEDFRHVRQEEVAILARALVGAGRSAVKLDQLLNVCTTNAL 1191
            PKWRMLRKIC+VHLFS KAL+DFRHVR+EEVAILARAL GAGRS V L QLLNVCTTNAL
Sbjct: 121  PKWRMLRKICSVHLFSNKALDDFRHVREEEVAILARALAGAGRSTVALGQLLNVCTTNAL 180

Query: 1190 ARVVLGRRVFGDGSGDTGPKADEF 1119
            ARV+LGRRVFGDGSG   PKADEF
Sbjct: 181  ARVMLGRRVFGDGSGGVDPKADEF 204



 Score =  257 bits (657), Expect(3) = 0.0
 Identities = 130/153 (84%), Positives = 143/153 (93%), Gaps = 3/153 (1%)
 Frame = -2

Query: 1081 DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQGITKRMKKLHAKFDSFLSMILEERKTG- 905
            DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQ +TK+MKKLH +FDSFL+ +LE+ K G 
Sbjct: 202  DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQSVTKKMKKLHLRFDSFLNTVLEDHKNGG 261

Query: 904  --VSGNLDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFIAGTDTSSSTIEWAIAELIR 731
              VSGN+DLLSTLISLKDDADGEGGKLSDIEIKALLLNLF AGTDTSSST+EWA+AELIR
Sbjct: 262  DVVSGNVDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFAAGTDTSSSTVEWAMAELIR 321

Query: 730  HPQLLKQAQEEMDTVVGQDRLVTELDLSQLTFL 632
            HPQL+KQAQEEMD VVG+DRLV+ELDLS+LTFL
Sbjct: 322  HPQLMKQAQEEMDIVVGRDRLVSELDLSRLTFL 354



 Score =  242 bits (617), Expect(3) = 0.0
 Identities = 112/134 (83%), Positives = 125/134 (93%)
 Frame = -3

Query: 558 ESCEVDGYYIPKESTLLVNVWAIARDPKIWTDPLEFRPARFLPGGEKSDVDVKGNDFEVI 379
           ESCEV+GYYIPK STLLVNVWAIARDPK+WTDPLEFRP+RFLPGGEK + +VKGNDFE+I
Sbjct: 378 ESCEVNGYYIPKGSTLLVNVWAIARDPKMWTDPLEFRPSRFLPGGEKPNANVKGNDFEII 437

Query: 378 PFGAGRRICAGLNMGLRMVQLVIAMLVHAIDWELADGLDAEKLNMEEAYGFALRRAAPLM 199
           PFGAGRRICAG+++GLRMVQL+IA LVHA DWELA+GLD EKLNMEEAYG  L+RAAPLM
Sbjct: 438 PFGAGRRICAGMSLGLRMVQLLIASLVHAFDWELANGLDPEKLNMEEAYGLTLQRAAPLM 497

Query: 198 VHPRPRLAPHVYKS 157
           VHP PRLAPH+YKS
Sbjct: 498 VHPSPRLAPHLYKS 511


>gb|ACN65826.1| flavonoid 3'-hydroxylase [Echinops bannaticus]
          Length = 508

 Score =  310 bits (793), Expect(3) = 0.0
 Identities = 156/206 (75%), Positives = 164/206 (79%), Gaps = 2/206 (0%)
 Frame = -1

Query: 1730 MTLLTLVLYACITALVFYAXXXXXXXXXXXXXP--GPTPWPIIGNLPHLGTSPHHSLAAL 1557
            MT+LT +LY CIT LVFYA                GPTPWPI+GNLPHLG  PHHSLA L
Sbjct: 1    MTILTFLLYTCITGLVFYALHLFNLRTPHRNRLPPGPTPWPIVGNLPHLGRVPHHSLADL 60

Query: 1556 ATKYGPLLHLRLGFXXXXXXXXXXXXAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAP 1377
            ATKYGPLLHLRLGF            AQFLK HDANFASRPPNSGAKHMAYNYQD+VFAP
Sbjct: 61   ATKYGPLLHLRLGFVDVVVAGSASVAAQFLKVHDANFASRPPNSGAKHMAYNYQDMVFAP 120

Query: 1376 YGPKWRMLRKICAVHLFSTKALEDFRHVRQEEVAILARALVGAGRSAVKLDQLLNVCTTN 1197
            YGPKWRMLRKIC+VHLFSTKAL+DFRHVRQEEVAILARALVGAG S VKL QLLNVCTTN
Sbjct: 121  YGPKWRMLRKICSVHLFSTKALDDFRHVRQEEVAILARALVGAGESTVKLGQLLNVCTTN 180

Query: 1196 ALARVVLGRRVFGDGSGDTGPKADEF 1119
            ALARV+LGRRVFGDGSG    K+DEF
Sbjct: 181  ALARVMLGRRVFGDGSGGGDSKSDEF 206



 Score =  249 bits (635), Expect(3) = 0.0
 Identities = 124/150 (82%), Positives = 139/150 (92%)
 Frame = -2

Query: 1081 DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQGITKRMKKLHAKFDSFLSMILEERKTGV 902
            DEFKDMV+E+MVLAGEFNIGDFIPALDWLDLQ +TK+MKKLH +FDSFL+ ILEE K   
Sbjct: 204  DEFKDMVMEMMVLAGEFNIGDFIPALDWLDLQSVTKKMKKLHVRFDSFLNTILEEHK--- 260

Query: 901  SGNLDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFIAGTDTSSSTIEWAIAELIRHPQ 722
            SGN+D +S LIS+KDDADG+GGKLSD EIKALLLNLF AGTDTSSST+EWAIAELIRHPQ
Sbjct: 261  SGNMDFVSRLISVKDDADGQGGKLSDTEIKALLLNLFAAGTDTSSSTVEWAIAELIRHPQ 320

Query: 721  LLKQAQEEMDTVVGQDRLVTELDLSQLTFL 632
            LLKQAQEEMDT+VG+DRLVTE+DLS+LTFL
Sbjct: 321  LLKQAQEEMDTIVGRDRLVTEVDLSRLTFL 350



 Score =  238 bits (606), Expect(3) = 0.0
 Identities = 108/135 (80%), Positives = 124/135 (91%)
 Frame = -3

Query: 558 ESCEVDGYYIPKESTLLVNVWAIARDPKIWTDPLEFRPARFLPGGEKSDVDVKGNDFEVI 379
           ++CEVDGYYIPK STLLVNVWAI+RDPKIW++PLEF+P RFLPGGEK D DVKGNDFE+I
Sbjct: 374 DTCEVDGYYIPKGSTLLVNVWAISRDPKIWSNPLEFQPTRFLPGGEKPDADVKGNDFELI 433

Query: 378 PFGAGRRICAGLNMGLRMVQLVIAMLVHAIDWELADGLDAEKLNMEEAYGFALRRAAPLM 199
           PFGAGRRICAG+++GL+MVQL+ A LVHA DWELA+GLD +KLNMEEAYG  L+RA PLM
Sbjct: 434 PFGAGRRICAGMSLGLKMVQLLTATLVHAFDWELANGLDPDKLNMEEAYGLTLQRATPLM 493

Query: 198 VHPRPRLAPHVYKSG 154
           VHPRPRLAPHVY+SG
Sbjct: 494 VHPRPRLAPHVYQSG 508


>gb|ACN65825.1| flavonoid 3'-hydroxylase [Cichorium intybus]
          Length = 511

 Score =  310 bits (793), Expect(3) = 0.0
 Identities = 152/204 (74%), Positives = 165/204 (80%)
 Frame = -1

Query: 1730 MTLLTLVLYACITALVFYAXXXXXXXXXXXXXPGPTPWPIIGNLPHLGTSPHHSLAALAT 1551
            MTLLTL++YAC+T +  Y              PGPTPWPI+GNLPHLGT PHHSLAALAT
Sbjct: 1    MTLLTLIIYACVTGIAAYVLLNLRNRRAKRLPPGPTPWPIVGNLPHLGTIPHHSLAALAT 60

Query: 1550 KYGPLLHLRLGFXXXXXXXXXXXXAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1371
            +YGPL+HLRLGF            AQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG
Sbjct: 61   RYGPLMHLRLGFVDVVVAASASVAAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 120

Query: 1370 PKWRMLRKICAVHLFSTKALEDFRHVRQEEVAILARALVGAGRSAVKLDQLLNVCTTNAL 1191
            P+WRMLRKIC+VHLFS K+L+DFRHVRQEEVAIL RALV AG+S V L QLLNVCTTNAL
Sbjct: 121  PRWRMLRKICSVHLFSAKSLDDFRHVRQEEVAILTRALVDAGKSTVILGQLLNVCTTNAL 180

Query: 1190 ARVVLGRRVFGDGSGDTGPKADEF 1119
            ARV+LGRRVFGDGSG   PKADEF
Sbjct: 181  ARVMLGRRVFGDGSGGGDPKADEF 204



 Score =  256 bits (655), Expect(3) = 0.0
 Identities = 133/153 (86%), Positives = 142/153 (92%), Gaps = 3/153 (1%)
 Frame = -2

Query: 1081 DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQGITKRMKKLHAKFDSFLSMILEERKTGV 902
            DEFKDMVVELMVLAGEFNIGDFIPALD LDLQG+TK+MKKLH +FDSFL+ ILEE KTG 
Sbjct: 202  DEFKDMVVELMVLAGEFNIGDFIPALDILDLQGVTKKMKKLHTRFDSFLNTILEEHKTGG 261

Query: 901  SG---NLDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFIAGTDTSSSTIEWAIAELIR 731
            SG   ++DLLSTLISLKDDADGEGGKLSD EIKALLLNLF AGTDTSSST+EWAIAELIR
Sbjct: 262  SGASAHVDLLSTLISLKDDADGEGGKLSDTEIKALLLNLFAAGTDTSSSTVEWAIAELIR 321

Query: 730  HPQLLKQAQEEMDTVVGQDRLVTELDLSQLTFL 632
            HP L+KQAQ+EMDTVVGQDRLVTELDLSQLTFL
Sbjct: 322  HPHLMKQAQQEMDTVVGQDRLVTELDLSQLTFL 354



 Score =  223 bits (569), Expect(3) = 0.0
 Identities = 103/134 (76%), Positives = 121/134 (90%)
 Frame = -3

Query: 558 ESCEVDGYYIPKESTLLVNVWAIARDPKIWTDPLEFRPARFLPGGEKSDVDVKGNDFEVI 379
           ESCE++GY IPK STLLVNVWAIARDPK+WT+PL+F+PARF+PGGEK + DVKGNDFEVI
Sbjct: 378 ESCEINGYNIPKGSTLLVNVWAIARDPKMWTNPLQFQPARFMPGGEKPNADVKGNDFEVI 437

Query: 378 PFGAGRRICAGLNMGLRMVQLVIAMLVHAIDWELADGLDAEKLNMEEAYGFALRRAAPLM 199
           PFGAGRRICAG+++GLRMVQL+ A LV A DWELA+GL+   LNMEEAYG  L+RAAPL+
Sbjct: 438 PFGAGRRICAGMSLGLRMVQLLTATLVQAFDWELANGLEPADLNMEEAYGLTLQRAAPLV 497

Query: 198 VHPRPRLAPHVYKS 157
           VHPRPRLAP+VYK+
Sbjct: 498 VHPRPRLAPYVYKT 511


>gb|ADK56126.1| putative flavonoid 3'-hydroxylase [Coreopsis grandiflora]
          Length = 512

 Score =  300 bits (769), Expect(3) = 0.0
 Identities = 148/204 (72%), Positives = 162/204 (79%)
 Frame = -1

Query: 1730 MTLLTLVLYACITALVFYAXXXXXXXXXXXXXPGPTPWPIIGNLPHLGTSPHHSLAALAT 1551
            MTLLTL++YAC+T +  Y              PGPTPWPI+GNLPHLGT PHHSLAALAT
Sbjct: 1    MTLLTLIIYACVTGIAAYVLLNLRNRRAKRLPPGPTPWPIVGNLPHLGTIPHHSLAALAT 60

Query: 1550 KYGPLLHLRLGFXXXXXXXXXXXXAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1371
            +YGPL+HLRLGF            AQFLKAHDANFASRP +SG KHMAYNYQDLVFAPYG
Sbjct: 61   RYGPLMHLRLGFVDVVVAASASVAAQFLKAHDANFASRPLSSGGKHMAYNYQDLVFAPYG 120

Query: 1370 PKWRMLRKICAVHLFSTKALEDFRHVRQEEVAILARALVGAGRSAVKLDQLLNVCTTNAL 1191
            P+WRMLRKIC+VHLF  K+L+DFRHVRQEEVAIL RALV AG+S V L QLLNVCTTNAL
Sbjct: 121  PRWRMLRKICSVHLFPAKSLDDFRHVRQEEVAILTRALVDAGKSTVILGQLLNVCTTNAL 180

Query: 1190 ARVVLGRRVFGDGSGDTGPKADEF 1119
            ARV+LGRRVFGDGSG   PKADEF
Sbjct: 181  ARVMLGRRVFGDGSGGGDPKADEF 204



 Score =  254 bits (649), Expect(3) = 0.0
 Identities = 132/153 (86%), Positives = 141/153 (92%), Gaps = 3/153 (1%)
 Frame = -2

Query: 1081 DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQGITKRMKKLHAKFDSFLSMILEERKTGV 902
            DEFKDMVVEL VLAGEFNIGDFIPALD LDLQG+TK+MKKLH +FDSFL+ ILEE KTG 
Sbjct: 202  DEFKDMVVELTVLAGEFNIGDFIPALDILDLQGVTKKMKKLHTRFDSFLNTILEEHKTGG 261

Query: 901  SG---NLDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFIAGTDTSSSTIEWAIAELIR 731
            SG   ++DLLSTLISLKDDADGEGGKLSD EIKALLLNLF AGTDTSSST+EWAIAELIR
Sbjct: 262  SGASAHVDLLSTLISLKDDADGEGGKLSDTEIKALLLNLFAAGTDTSSSTVEWAIAELIR 321

Query: 730  HPQLLKQAQEEMDTVVGQDRLVTELDLSQLTFL 632
            HP L+KQAQ+EMDTVVGQDRLVTELDLSQLTFL
Sbjct: 322  HPHLMKQAQQEMDTVVGQDRLVTELDLSQLTFL 354



 Score =  228 bits (581), Expect(3) = 0.0
 Identities = 105/135 (77%), Positives = 122/135 (90%)
 Frame = -3

Query: 558 ESCEVDGYYIPKESTLLVNVWAIARDPKIWTDPLEFRPARFLPGGEKSDVDVKGNDFEVI 379
           ESCE++GY IPK STLLVNVWAIARDPK+WT+PL+F+PARF+PGGEK + DVKGNDFEVI
Sbjct: 378 ESCEINGYNIPKGSTLLVNVWAIARDPKMWTNPLQFQPARFMPGGEKPNADVKGNDFEVI 437

Query: 378 PFGAGRRICAGLNMGLRMVQLVIAMLVHAIDWELADGLDAEKLNMEEAYGFALRRAAPLM 199
           PFGAGRRICAG+++GLRMVQL+ A LV A DWELA+GL+   LNMEEAYG  L+RAAPL+
Sbjct: 438 PFGAGRRICAGMSLGLRMVQLLTATLVQAFDWELANGLEPADLNMEEAYGLTLQRAAPLV 497

Query: 198 VHPRPRLAPHVYKSG 154
           VHPRPRLAPHVYK+G
Sbjct: 498 VHPRPRLAPHVYKTG 512


>gb|ACO35753.1| flavonoid 3'-hydroxylase [Bidens aurea]
          Length = 512

 Score =  302 bits (774), Expect(3) = 0.0
 Identities = 146/204 (71%), Positives = 165/204 (80%)
 Frame = -1

Query: 1730 MTLLTLVLYACITALVFYAXXXXXXXXXXXXXPGPTPWPIIGNLPHLGTSPHHSLAALAT 1551
            MT+L+L+LY  +TA++ Y              PGPTPWP++GNLPHLGT PHHSLAALA 
Sbjct: 1    MTILSLLLYTSLTAIILYLLHNLLTRHTNRLPPGPTPWPVVGNLPHLGTVPHHSLAALAA 60

Query: 1550 KYGPLLHLRLGFXXXXXXXXXXXXAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1371
            KYGPL+HLRLGF            +QFLK HDANFASRPPNSGA+H+AYNYQDLVFAPYG
Sbjct: 61   KYGPLMHLRLGFVDVVVAASASVASQFLKTHDANFASRPPNSGAEHIAYNYQDLVFAPYG 120

Query: 1370 PKWRMLRKICAVHLFSTKALEDFRHVRQEEVAILARALVGAGRSAVKLDQLLNVCTTNAL 1191
            P+WRMLRKIC+VHLFS KAL+D+RHVRQEEVAIL RALVGAG++ VKL QLLNVCTTNAL
Sbjct: 121  PRWRMLRKICSVHLFSGKALDDYRHVRQEEVAILTRALVGAGKAPVKLGQLLNVCTTNAL 180

Query: 1190 ARVVLGRRVFGDGSGDTGPKADEF 1119
            ARV+LGRRVFGDGSG   PKADEF
Sbjct: 181  ARVMLGRRVFGDGSGGGDPKADEF 204



 Score =  249 bits (635), Expect(3) = 0.0
 Identities = 127/153 (83%), Positives = 140/153 (91%), Gaps = 3/153 (1%)
 Frame = -2

Query: 1081 DEFKDMVVELMVLAGEFNIGDFIPALDWLDLQGITKRMKKLHAKFDSFLSMILEERKTGV 902
            DEFKDMVVE+MVLAGEFNIGDFIPALDWLDLQGITK+MKKLHA+FDSFL+ ILEE K G 
Sbjct: 202  DEFKDMVVEMMVLAGEFNIGDFIPALDWLDLQGITKKMKKLHARFDSFLNTILEEHKPGK 261

Query: 901  SG---NLDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFIAGTDTSSSTIEWAIAELIR 731
             G   + DLLSTLISLKDDADGEGGKLSDIEIKALLLNLF+AGTDTSSS +EWA+AELIR
Sbjct: 262  GGASSHKDLLSTLISLKDDADGEGGKLSDIEIKALLLNLFVAGTDTSSSKVEWAMAELIR 321

Query: 730  HPQLLKQAQEEMDTVVGQDRLVTELDLSQLTFL 632
            +P++L+QAQEEMD VVG+DRLVTE DL QLTFL
Sbjct: 322  NPKILRQAQEEMDNVVGRDRLVTESDLGQLTFL 354



 Score =  228 bits (580), Expect(3) = 0.0
 Identities = 106/135 (78%), Positives = 119/135 (88%)
 Frame = -3

Query: 558 ESCEVDGYYIPKESTLLVNVWAIARDPKIWTDPLEFRPARFLPGGEKSDVDVKGNDFEVI 379
           ESCEVDGYYIPK STLLVNVWAIARDPK+WTDPLEFRP RFLPGGEK +VDVKGNDFEVI
Sbjct: 378 ESCEVDGYYIPKGSTLLVNVWAIARDPKMWTDPLEFRPTRFLPGGEKPNVDVKGNDFEVI 437

Query: 378 PFGAGRRICAGLNMGLRMVQLVIAMLVHAIDWELADGLDAEKLNMEEAYGFALRRAAPLM 199
           PFGAGRRIC G+++GLRMVQL++A LV   DWELA+G+  EKLNM EAYG  L+RA PL+
Sbjct: 438 PFGAGRRICVGISLGLRMVQLLVATLVQTFDWELANGVQPEKLNMNEAYGLTLQRAEPLI 497

Query: 198 VHPRPRLAPHVYKSG 154
           VHP+PRLAP V +SG
Sbjct: 498 VHPKPRLAPDVDESG 512