BLASTX nr result
ID: Atractylodes21_contig00001556
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes21_contig00001556
(1831 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ACN65825.1| flavonoid 3'-hydroxylase [Cichorium intybus] 352 e-177
gb|ACN65826.1| flavonoid 3'-hydroxylase [Echinops bannaticus] 350 e-177
gb|ADM26615.1| flavonoid 3'-hydroxylase [Cynara cardunculus var.... 342 e-175
gb|AEA06595.1| flavonoid 3'-hydroxylase [Chromolaena odorata] 345 e-174
gb|ABM46853.1| flavonoid 3'-hydroxylase [Ageratina adenophora] 350 e-174
>gb|ACN65825.1| flavonoid 3'-hydroxylase [Cichorium intybus]
Length = 511
Score = 352 bits (904), Expect(2) = e-177
Identities = 169/208 (81%), Positives = 181/208 (87%)
Frame = -2
Query: 1797 MTLLTLVLYACITALVFYALLNLRTRHPNRLPPGPTPWPIIGNLPHLGTSPHHSLAALAT 1618
MTLLTL++YAC+T + Y LLNLR R RLPPGPTPWPI+GNLPHLGT PHHSLAALAT
Sbjct: 1 MTLLTLIIYACVTGIAAYVLLNLRNRRAKRLPPGPTPWPIVGNLPHLGTIPHHSLAALAT 60
Query: 1617 KYGPLMHLRFGFVDXXXXXXXXXAEQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1438
+YGPLMHLR GFVD A QFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG
Sbjct: 61 RYGPLMHLRLGFVDVVVAASASVAAQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 120
Query: 1437 PKWRLLRKICSVHLFSTKALDDFRHVRQEEVAILARALAVAGRSTVKLDQLLNVCTTNAL 1258
P+WR+LRKICSVHLFS K+LDDFRHVRQEEVAIL RAL AG+STV L QLLNVCTTNAL
Sbjct: 121 PRWRMLRKICSVHLFSAKSLDDFRHVRQEEVAILTRALVDAGKSTVILGQLLNVCTTNAL 180
Query: 1257 ARMVLGRRVFGDGSGNGDPKADEFKDMV 1174
AR++LGRRVFGDGSG GDPKADEFKDMV
Sbjct: 181 ARVMLGRRVFGDGSGGGDPKADEFKDMV 208
Score = 298 bits (763), Expect(2) = e-177
Identities = 152/176 (86%), Positives = 164/176 (93%), Gaps = 3/176 (1%)
Frame = -3
Query: 1112 ELMVLAGEFNIGDFIPALDWLDLQGITKKMKKLHARFDLFFNTILEEHKAGVSG---NLD 942
ELMVLAGEFNIGDFIPALD LDLQG+TKKMKKLH RFD F NTILEEHK G SG ++D
Sbjct: 210 ELMVLAGEFNIGDFIPALDILDLQGVTKKMKKLHTRFDSFLNTILEEHKTGGSGASAHVD 269
Query: 941 LLSTLISLKDDADGEGGKLSDNEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPQLLKQA 762
LLSTLISLKDDADGEGGKLSD EIKALLLNLF AGTDTSSSTVEWAIAELIRHP L+KQA
Sbjct: 270 LLSTLISLKDDADGEGGKLSDTEIKALLLNLFAAGTDTSSSTVEWAIAELIRHPHLMKQA 329
Query: 761 QEEMDTVVGRDQLVTDLDLSQLTFLQAIVKETFRLHPSTPLSLPRIASDSCKVDGY 594
Q+EMDTVVG+D+LVT+LDLSQLTFLQAIVKETFRLHPSTPLSLPRIAS+SC+++GY
Sbjct: 330 QQEMDTVVGQDRLVTELDLSQLTFLQAIVKETFRLHPSTPLSLPRIASESCEINGY 385
Score = 159 bits (402), Expect = 2e-36
Identities = 76/86 (88%), Positives = 83/86 (96%)
Frame = -2
Query: 423 DADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWELANGLDPEKLNMEEA 244
+ADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLV AFDWELANGL+P LNMEEA
Sbjct: 426 NADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVQAFDWELANGLEPADLNMEEA 485
Query: 243 FGLTLQRAAPLMVHPRPRLSPHVYKS 166
+GLTLQRAAPL+VHPRPRL+P+VYK+
Sbjct: 486 YGLTLQRAAPLVVHPRPRLAPYVYKT 511
>gb|ACN65826.1| flavonoid 3'-hydroxylase [Echinops bannaticus]
Length = 508
Score = 350 bits (899), Expect(2) = e-177
Identities = 172/210 (81%), Positives = 181/210 (86%), Gaps = 2/210 (0%)
Frame = -2
Query: 1797 MTLLTLVLYACITALVFYAL--LNLRTRHPNRLPPGPTPWPIIGNLPHLGTSPHHSLAAL 1624
MT+LT +LY CIT LVFYAL NLRT H NRLPPGPTPWPI+GNLPHLG PHHSLA L
Sbjct: 1 MTILTFLLYTCITGLVFYALHLFNLRTPHRNRLPPGPTPWPIVGNLPHLGRVPHHSLADL 60
Query: 1623 ATKYGPLMHLRFGFVDXXXXXXXXXAEQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAP 1444
ATKYGPL+HLR GFVD A QFLK HDANFASRPPNSGAKHMAYNYQD+VFAP
Sbjct: 61 ATKYGPLLHLRLGFVDVVVAGSASVAAQFLKVHDANFASRPPNSGAKHMAYNYQDMVFAP 120
Query: 1443 YGPKWRLLRKICSVHLFSTKALDDFRHVRQEEVAILARALAVAGRSTVKLDQLLNVCTTN 1264
YGPKWR+LRKICSVHLFSTKALDDFRHVRQEEVAILARAL AG STVKL QLLNVCTTN
Sbjct: 121 YGPKWRMLRKICSVHLFSTKALDDFRHVRQEEVAILARALVGAGESTVKLGQLLNVCTTN 180
Query: 1263 ALARMVLGRRVFGDGSGNGDPKADEFKDMV 1174
ALAR++LGRRVFGDGSG GD K+DEFKDMV
Sbjct: 181 ALARVMLGRRVFGDGSGGGDSKSDEFKDMV 210
Score = 300 bits (767), Expect(2) = e-177
Identities = 149/174 (85%), Positives = 162/174 (93%)
Frame = -3
Query: 1112 ELMVLAGEFNIGDFIPALDWLDLQGITKKMKKLHARFDLFFNTILEEHKAGVSGNLDLLS 933
E+MVLAGEFNIGDFIPALDWLDLQ +TKKMKKLH RFD F NTILEEHK SGN+D +S
Sbjct: 212 EMMVLAGEFNIGDFIPALDWLDLQSVTKKMKKLHVRFDSFLNTILEEHK---SGNMDFVS 268
Query: 932 TLISLKDDADGEGGKLSDNEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPQLLKQAQEE 753
LIS+KDDADG+GGKLSD EIKALLLNLF AGTDTSSSTVEWAIAELIRHPQLLKQAQEE
Sbjct: 269 RLISVKDDADGQGGKLSDTEIKALLLNLFAAGTDTSSSTVEWAIAELIRHPQLLKQAQEE 328
Query: 752 MDTVVGRDQLVTDLDLSQLTFLQAIVKETFRLHPSTPLSLPRIASDSCKVDGYY 591
MDT+VGRD+LVT++DLS+LTFLQAIVKE FRLHPSTPLSLPRIASD+C+VDGYY
Sbjct: 329 MDTIVGRDRLVTEVDLSRLTFLQAIVKEIFRLHPSTPLSLPRIASDTCEVDGYY 382
Score = 171 bits (433), Expect = 6e-40
Identities = 80/87 (91%), Positives = 86/87 (98%)
Frame = -2
Query: 423 DADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWELANGLDPEKLNMEEA 244
DADVKGNDFE+IPFGAGRRICAGMSLGL+MVQLLTATLVHAFDWELANGLDP+KLNMEEA
Sbjct: 422 DADVKGNDFELIPFGAGRRICAGMSLGLKMVQLLTATLVHAFDWELANGLDPDKLNMEEA 481
Query: 243 FGLTLQRAAPLMVHPRPRLSPHVYKSG 163
+GLTLQRA PLMVHPRPRL+PHVY+SG
Sbjct: 482 YGLTLQRATPLMVHPRPRLAPHVYQSG 508
>gb|ADM26615.1| flavonoid 3'-hydroxylase [Cynara cardunculus var. scolymus]
Length = 512
Score = 342 bits (876), Expect(2) = e-175
Identities = 168/208 (80%), Positives = 179/208 (86%)
Frame = -2
Query: 1797 MTLLTLVLYACITALVFYALLNLRTRHPNRLPPGPTPWPIIGNLPHLGTSPHHSLAALAT 1618
MT+L L+LYA IT L+ Y LLNLRT NRLPPGPTPWPIIGNLPHLG PHH+LAA+AT
Sbjct: 1 MTILPLLLYASITGLLLYVLLNLRTPRSNRLPPGPTPWPIIGNLPHLGRIPHHALAAMAT 60
Query: 1617 KYGPLMHLRFGFVDXXXXXXXXXAEQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1438
KYGPLMHLR G VD A QFLK HDANFASRPPNSGAKH+AY+YQDLVFAPYG
Sbjct: 61 KYGPLMHLRLGVVDVVVAASASVAAQFLKVHDANFASRPPNSGAKHIAYDYQDLVFAPYG 120
Query: 1437 PKWRLLRKICSVHLFSTKALDDFRHVRQEEVAILARALAVAGRSTVKLDQLLNVCTTNAL 1258
PKWR+LRKICSVHLFS KALDDFRHVR+EEVAILARALA AGRSTV L QLLNVCTTNAL
Sbjct: 121 PKWRMLRKICSVHLFSNKALDDFRHVREEEVAILARALAGAGRSTVALGQLLNVCTTNAL 180
Query: 1257 ARMVLGRRVFGDGSGNGDPKADEFKDMV 1174
AR++LGRRVFGDGSG DPKADEFKDMV
Sbjct: 181 ARVMLGRRVFGDGSGGVDPKADEFKDMV 208
Score = 302 bits (773), Expect(2) = e-175
Identities = 152/177 (85%), Positives = 166/177 (93%), Gaps = 3/177 (1%)
Frame = -3
Query: 1112 ELMVLAGEFNIGDFIPALDWLDLQGITKKMKKLHARFDLFFNTILEEHKAG---VSGNLD 942
ELMVLAGEFNIGDFIPALDWLDLQ +TKKMKKLH RFD F NT+LE+HK G VSGN+D
Sbjct: 210 ELMVLAGEFNIGDFIPALDWLDLQSVTKKMKKLHLRFDSFLNTVLEDHKNGGDVVSGNVD 269
Query: 941 LLSTLISLKDDADGEGGKLSDNEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPQLLKQA 762
LLSTLISLKDDADGEGGKLSD EIKALLLNLF AGTDTSSSTVEWA+AELIRHPQL+KQA
Sbjct: 270 LLSTLISLKDDADGEGGKLSDIEIKALLLNLFAAGTDTSSSTVEWAMAELIRHPQLMKQA 329
Query: 761 QEEMDTVVGRDQLVTDLDLSQLTFLQAIVKETFRLHPSTPLSLPRIASDSCKVDGYY 591
QEEMD VVGRD+LV++LDLS+LTFL+AIVKETFRLHPSTPLSLPRIAS+SC+V+GYY
Sbjct: 330 QEEMDIVVGRDRLVSELDLSRLTFLEAIVKETFRLHPSTPLSLPRIASESCEVNGYY 386
Score = 164 bits (414), Expect = 1e-37
Identities = 77/86 (89%), Positives = 84/86 (97%)
Frame = -2
Query: 423 DADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWELANGLDPEKLNMEEA 244
+A+VKGNDFE+IPFGAGRRICAGMSLGLRMVQLL A+LVHAFDWELANGLDPEKLNMEEA
Sbjct: 426 NANVKGNDFEIIPFGAGRRICAGMSLGLRMVQLLIASLVHAFDWELANGLDPEKLNMEEA 485
Query: 243 FGLTLQRAAPLMVHPRPRLSPHVYKS 166
+GLTLQRAAPLMVHP PRL+PH+YKS
Sbjct: 486 YGLTLQRAAPLMVHPSPRLAPHLYKS 511
>gb|AEA06595.1| flavonoid 3'-hydroxylase [Chromolaena odorata]
Length = 507
Score = 345 bits (885), Expect(2) = e-174
Identities = 167/208 (80%), Positives = 178/208 (85%)
Frame = -2
Query: 1797 MTLLTLVLYACITALVFYALLNLRTRHPNRLPPGPTPWPIIGNLPHLGTSPHHSLAALAT 1618
M++L +LY ITALVFY LLNLR RH RLPPGP+PWPI+GNLPHLGT PHHSLAALA
Sbjct: 1 MSILISLLYTFITALVFYLLLNLRPRHSKRLPPGPSPWPIVGNLPHLGTIPHHSLAALAK 60
Query: 1617 KYGPLMHLRFGFVDXXXXXXXXXAEQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1438
KYGPLMHLR GFVD A QFLK HDANFASRPPNSGAKH+AYNY DLVFAPYG
Sbjct: 61 KYGPLMHLRLGFVDVVVAASASVAAQFLKTHDANFASRPPNSGAKHIAYNYHDLVFAPYG 120
Query: 1437 PKWRLLRKICSVHLFSTKALDDFRHVRQEEVAILARALAVAGRSTVKLDQLLNVCTTNAL 1258
P+WR+LRKICSVHLFSTKALDDFRH+RQEEVAIL R L AG STVKL QLLNVCTTNAL
Sbjct: 121 PRWRMLRKICSVHLFSTKALDDFRHIRQEEVAILTRVLVGAGESTVKLGQLLNVCTTNAL 180
Query: 1257 ARMVLGRRVFGDGSGNGDPKADEFKDMV 1174
AR++LGRRVFGDGSG GDPKADEFKDMV
Sbjct: 181 ARVMLGRRVFGDGSGTGDPKADEFKDMV 208
Score = 296 bits (758), Expect(2) = e-174
Identities = 150/174 (86%), Positives = 161/174 (92%)
Frame = -3
Query: 1112 ELMVLAGEFNIGDFIPALDWLDLQGITKKMKKLHARFDLFFNTILEEHKAGVSGNLDLLS 933
ELMVLAGEFNIGDFIPALDWLDLQG+TKKMKKLH RFD F NTILEEHK G +G DLLS
Sbjct: 210 ELMVLAGEFNIGDFIPALDWLDLQGVTKKMKKLHTRFDSFLNTILEEHKTGNNG--DLLS 267
Query: 932 TLISLKDDADGEGGKLSDNEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPQLLKQAQEE 753
TLI+LKDDAD EGGKLSD EIKALLLNLF AGTDTSSSTVEWAIAELIR PQLLKQAQEE
Sbjct: 268 TLIALKDDADEEGGKLSDIEIKALLLNLFAAGTDTSSSTVEWAIAELIRQPQLLKQAQEE 327
Query: 752 MDTVVGRDQLVTDLDLSQLTFLQAIVKETFRLHPSTPLSLPRIASDSCKVDGYY 591
+DT+VGRD+LVT+LDLSQLTFLQAIVKETFRLHPSTPLSLPR++SD C+V GY+
Sbjct: 328 IDTIVGRDRLVTELDLSQLTFLQAIVKETFRLHPSTPLSLPRVSSDDCEVSGYH 381
Score = 141 bits (356), Expect = 5e-31
Identities = 67/86 (77%), Positives = 75/86 (87%)
Frame = -2
Query: 423 DADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWELANGLDPEKLNMEEA 244
+ DV+GNDFEVIPFGAGRRIC G+SLGLRMVQLL ATLV FDWELA GL+PEKLNM+E
Sbjct: 421 NVDVRGNDFEVIPFGAGRRICVGISLGLRMVQLLVATLVQTFDWELAKGLEPEKLNMDET 480
Query: 243 FGLTLQRAAPLMVHPRPRLSPHVYKS 166
+GLTLQRA PLMVHP+ RL+ HV KS
Sbjct: 481 YGLTLQRAEPLMVHPKSRLAHHVCKS 506
>gb|ABM46853.1| flavonoid 3'-hydroxylase [Ageratina adenophora]
Length = 510
Score = 350 bits (897), Expect(2) = e-174
Identities = 168/208 (80%), Positives = 181/208 (87%)
Frame = -2
Query: 1797 MTLLTLVLYACITALVFYALLNLRTRHPNRLPPGPTPWPIIGNLPHLGTSPHHSLAALAT 1618
MT+LTL+LY I+AL FY LLNLRTRHP RLPPGPTPWPI+GNLPHLGT PHHSLAALAT
Sbjct: 1 MTILTLLLYTSISALAFYLLLNLRTRHPKRLPPGPTPWPIVGNLPHLGTVPHHSLAALAT 60
Query: 1617 KYGPLMHLRFGFVDXXXXXXXXXAEQFLKAHDANFASRPPNSGAKHMAYNYQDLVFAPYG 1438
KYGPLMHLR GFVD A QFLK+HDANFASRPPNSGAKH+AYNY DLVFAPYG
Sbjct: 61 KYGPLMHLRLGFVDVVVAASASVASQFLKSHDANFASRPPNSGAKHIAYNYHDLVFAPYG 120
Query: 1437 PKWRLLRKICSVHLFSTKALDDFRHVRQEEVAILARALAVAGRSTVKLDQLLNVCTTNAL 1258
P+WR+LRKICSVHLFS K+LDDFRHVRQEEVAIL R L AG +TVKL QLLNVCTTNAL
Sbjct: 121 PRWRMLRKICSVHLFSAKSLDDFRHVRQEEVAILTRVLISAGETTVKLGQLLNVCTTNAL 180
Query: 1257 ARMVLGRRVFGDGSGNGDPKADEFKDMV 1174
AR++LGRRVFGDGS + DPKADEFKDMV
Sbjct: 181 ARVLLGRRVFGDGSRDSDPKADEFKDMV 208
Score = 291 bits (744), Expect(2) = e-174
Identities = 151/177 (85%), Positives = 165/177 (93%), Gaps = 3/177 (1%)
Frame = -3
Query: 1112 ELMVLAGEFNIGDFIPALDWLDLQGITKKMKKLHARFDLFFNTILEEHKA-GVSGNLDLL 936
E+MVLAGEFNIGDFIPALDWLDLQG+TKKMKKLH RFD FFN ILEEHK+ G SG DLL
Sbjct: 210 EMMVLAGEFNIGDFIPALDWLDLQGVTKKMKKLHTRFDSFFNLILEEHKSYGNSG--DLL 267
Query: 935 STLISLKDDAD--GEGGKLSDNEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPQLLKQA 762
STLI LKDDAD GEG KL+D EIKALLLNLFTAGTDTSSSTVEWAIAELIRHP+LLKQA
Sbjct: 268 STLIGLKDDADADGEGVKLTDIEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPRLLKQA 327
Query: 761 QEEMDTVVGRDQLVTDLDLSQLTFLQAIVKETFRLHPSTPLSLPRIASDSCKVDGYY 591
QEE+DTVVGRD+LVT+LDL+QLTF+QAIVKETFRLHPSTPLSLPRI+S++C+VDGYY
Sbjct: 328 QEEIDTVVGRDRLVTELDLNQLTFIQAIVKETFRLHPSTPLSLPRISSEACEVDGYY 384
Score = 143 bits (361), Expect = 1e-31
Identities = 67/86 (77%), Positives = 77/86 (89%)
Frame = -2
Query: 423 DADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWELANGLDPEKLNMEEA 244
+ DV+GNDFEVIPFGAGRRIC GM+LGLRMVQLL ATLV FDWELA GL+PEKLNM+EA
Sbjct: 424 NVDVRGNDFEVIPFGAGRRICVGMTLGLRMVQLLVATLVQTFDWELAKGLEPEKLNMDEA 483
Query: 243 FGLTLQRAAPLMVHPRPRLSPHVYKS 166
+GLTLQRA L+VHP+PRL+P VY+S
Sbjct: 484 YGLTLQRAEQLIVHPKPRLAPGVYES 509