BLASTX nr result
ID: Astragalus24_contig00010917
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus24_contig00010917
(1206 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ATY39976.1| Isoflavone O-methyltransferase [Astragalus membra... 631 0.0
sp|Q84KK5.1|D7OMT_GLYEC RecName: Full=Isoflavone 7-O-methyltrans... 534 0.0
gb|AEF14418.1| isoflavone O-methyltranferase [Onobrychis viciifo... 517 0.0
ref|XP_004489528.1| PREDICTED: isoflavone-7-O-methyltransferase ... 516 0.0
ref|XP_003621488.2| isoflavone-7-O-methyltransferase [Medicago t... 512 e-179
sp|O22309.1|7OMT9_MEDSA RecName: Full=Isoflavone-7-O-methyltrans... 511 e-179
sp|O22308.1|7OMT6_MEDSA RecName: Full=Isoflavone-7-O-methyltrans... 509 e-178
sp|O24529.1|7OMT8_MEDSA RecName: Full=Isoflavone-7-O-methyltrans... 508 e-177
dbj|GAU19866.1| hypothetical protein TSUD_170920 [Trifolium subt... 498 e-173
gb|PNX99091.1| isoflavone 7-O-methyltransferase-like protein [Tr... 496 e-173
ref|XP_003621484.1| isoflavone-7-O-methyltransferase [Medicago t... 489 e-170
pdb|1FPX|A Chain A, Crystal Structure Analysis Of Selenomethioni... 488 e-170
dbj|GAU19865.1| hypothetical protein TSUD_170910 [Trifolium subt... 486 e-169
pdb|6CIG|A Chain A, CRYSTAL STRUCTURE ANALYSIS OF SELENOMETHIONI... 484 e-168
gb|AAB88294.1| o-methytransferase [Medicago sativa] 484 e-168
ref|NP_001241162.1| isoflavone 7-O-methyltransferase-like [Glyci... 480 e-166
gb|ABD83942.1| IOMT 2 [Medicago truncatula] 478 e-166
ref|XP_020219848.1| isoflavone 7-O-methyltransferase-like [Cajan... 476 e-165
ref|XP_020219068.1| isoflavone 7-O-methyltransferase-like [Cajan... 475 e-165
ref|NP_001241010.1| uncharacterized protein LOC100812688 [Glycin... 469 e-162
>gb|ATY39976.1| Isoflavone O-methyltransferase [Astragalus membranaceus]
Length = 362
Score = 631 bits (1627), Expect = 0.0
Identities = 320/362 (88%), Positives = 321/362 (88%), Gaps = 3/362 (0%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI
Sbjct: 1 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXX---AYALTVASELLVKGTNLC 436
LKVPQTK+GNVQRLMRYLAHNGFFEIVRNH AYALTVASELLVKGTNLC
Sbjct: 61 LKVPQTKVGNVQRLMRYLAHNGFFEIVRNHDDDDDDEEEEEEAYALTVASELLVKGTNLC 120
Query: 437 LAPMVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMAS 616
LAPMVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMAS
Sbjct: 121 LAPMVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMAS 180
Query: 617 DSQMINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVT 796
DSQMINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVT
Sbjct: 181 DSQMINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVT 240
Query: 797 NNVSCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXX 976
NNVSCVGGDMFISVPKADAVLLKYILHNWTD A
Sbjct: 241 NNVSCVGGDMFISVPKADAVLLKYILHNWTDKDCIKILEKCKEAISSDGKKGKVIIIDIV 300
Query: 977 XNEKKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEI 1156
NEKKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEI
Sbjct: 301 INEKKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEI 360
Query: 1157 YP 1162
YP
Sbjct: 361 YP 362
>sp|Q84KK5.1|D7OMT_GLYEC RecName: Full=Isoflavone 7-O-methyltransferase; AltName:
Full=Daidzein 7-O-methyltransferase
dbj|BAC58012.1| S-adenosyl-L-methionine: daidzein 7-0-methyltransferase [Glycyrrhiza
echinata]
Length = 357
Score = 534 bits (1375), Expect = 0.0
Identities = 266/359 (74%), Positives = 295/359 (82%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGRK SEIF+GQ LLY+H+YAFIDSMCLKW +ELDIPNIIHNHGKPIT+ ELVSI
Sbjct: 1 MASSINGRKPSEIFQGQALLYRHIYAFIDSMCLKWIVELDIPNIIHNHGKPITVSELVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
LKVPQTK GNVQR+MRY+AHNGFFE VR AYALT ASELLVKG+ LCLAP
Sbjct: 61 LKVPQTKAGNVQRIMRYMAHNGFFERVR--IQEEQEENEAYALTAASELLVKGSELCLAP 118
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYHQLKKWIYEEDLTLFGV+LGSHF+EFLN NPEYNKSFN+AMASDSQ
Sbjct: 119 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVSLGSHFWEFLNENPEYNKSFNDAMASDSQ 178
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
MINLAL+DCNS FEG+ESIVDVGGG GTTAK+IC+TFP LKCIVFDRP+VVE+LS TNN+
Sbjct: 179 MINLALRDCNSGFEGVESIVDVGGGIGTTAKIICDTFPNLKCIVFDRPKVVENLSGTNNL 238
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
S VGGDMF SVPKADAVLLK+ILHNWTD A NE
Sbjct: 239 SYVGGDMFQSVPKADAVLLKWILHNWTDNDCRRILEKCKEAVSSDGEKGKVIIIEMVINE 298
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
+D+H ++ KLLMDVNMACLNG ER+ EEWKKLF +AGF+D+KISPLTG LS+IE+YP
Sbjct: 299 NQDEHEITGTKLLMDVNMACLNGKERSEEEWKKLFIEAGFRDYKISPLTGFLSLIEVYP 357
>gb|AEF14418.1| isoflavone O-methyltranferase [Onobrychis viciifolia]
Length = 361
Score = 517 bits (1332), Expect = 0.0
Identities = 258/355 (72%), Positives = 288/355 (81%)
Frame = +2
Query: 98 INGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSILKVP 277
INGRKASEIF+GQ LLYKH+YAFIDSMCLK +ELDIPNIIHNHGKPI L ELVSILKVP
Sbjct: 11 INGRKASEIFQGQALLYKHIYAFIDSMCLKCIVELDIPNIIHNHGKPIPLPELVSILKVP 70
Query: 278 QTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAPMVEC 457
+TK N+ R+MRY++HNGFF IV+ AYALT ASELLVKG++LCLAPMVEC
Sbjct: 71 ETKFDNLGRIMRYMSHNGFFVIVKTQEETEE----AYALTAASELLVKGSDLCLAPMVEC 126
Query: 458 VLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQMINL 637
VLDPTLSGSYHQLKKWIYEEDLTLFGV+LGSHFYEFLN NP+YN SFNEAMASDS MI+L
Sbjct: 127 VLDPTLSGSYHQLKKWIYEEDLTLFGVSLGSHFYEFLNNNPDYNSSFNEAMASDSHMIHL 186
Query: 638 ALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNVSCVG 817
AL+DCN VFEG+ESIVDVGGGTG TAK IC+ FP+LKCIVFDRPQVVE+LS +NN++ VG
Sbjct: 187 ALRDCNFVFEGVESIVDVGGGTGITAKTICDAFPKLKCIVFDRPQVVENLSGSNNLTYVG 246
Query: 818 GDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNEKKDD 997
GDMF S+PKA+AVLLKYILHNWTD A NE KDD
Sbjct: 247 GDMFQSIPKAEAVLLKYILHNWTDKDCIKILKKCKEAISSDGKKGKVILLDMVINENKDD 306
Query: 998 HVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
L+Q+KLLMDV MACLNG ERT EEW KLFT+AGFQD+KISPLTG+LS+IEIYP
Sbjct: 307 QRLTQIKLLMDVTMACLNGRERTEEEWSKLFTQAGFQDYKISPLTGLLSLIEIYP 361
>ref|XP_004489528.1| PREDICTED: isoflavone-7-O-methyltransferase 9 [Cicer arietinum]
Length = 357
Score = 516 bits (1329), Expect = 0.0
Identities = 255/357 (71%), Positives = 292/357 (81%)
Frame = +2
Query: 92 SPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSILK 271
S ING EIF+ Q LLYKH++AFIDSMCLKWT+E++IPNIIHNH KPITL LVSIL+
Sbjct: 4 SLINGHNPREIFQAQALLYKHIFAFIDSMCLKWTVEMNIPNIIHNHNKPITLSNLVSILQ 63
Query: 272 VPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAPMV 451
VP +KIGNVQRLMRYLAHNGFFEI+ N +YALTVAS+LLVKG++LCLAPMV
Sbjct: 64 VPSSKIGNVQRLMRYLAHNGFFEIITNQEEENEE---SYALTVASKLLVKGSDLCLAPMV 120
Query: 452 ECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQMI 631
ECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGS F++FLN NPEYNKSFNEAMASDS+MI
Sbjct: 121 ECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSGFWDFLNKNPEYNKSFNEAMASDSKMI 180
Query: 632 NLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNVSC 811
NLAL+DCN VFEGLES+VDVGGGTGTTAK+IC+TFP+LKC+VFDRPQVV++L +NN+S
Sbjct: 181 NLALRDCNLVFEGLESMVDVGGGTGTTAKIICDTFPKLKCVVFDRPQVVDNLCGSNNLSY 240
Query: 812 VGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNEKK 991
VGGDMF SVP ADAVLLK+ILHNWTD A NEKK
Sbjct: 241 VGGDMFSSVPNADAVLLKWILHNWTDKDCIRILEKCKEAVTSNGKKGKVIVIDMVINEKK 300
Query: 992 DDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
D++ ++Q+KLLMDVNMACLNG ER +EWKKLF +AGFQD+KISPLTG LS++EIYP
Sbjct: 301 DENQITQIKLLMDVNMACLNGKERNEKEWKKLFIEAGFQDYKISPLTGFLSLMEIYP 357
>ref|XP_003621488.2| isoflavone-7-O-methyltransferase [Medicago truncatula]
gb|AAY18582.1| SAM dependent isoflavone 7-O-methyltransferase [Medicago truncatula]
gb|AES77706.2| isoflavone-7-O-methyltransferase [Medicago truncatula]
Length = 352
Score = 512 bits (1319), Expect = e-179
Identities = 252/359 (70%), Positives = 294/359 (81%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS +NGRK SEIF+ Q LLYKH++AFIDSM LKW +E++IPNIIHNHGKPI+L LVSI
Sbjct: 1 MASSLNGRKPSEIFKAQALLYKHIFAFIDSMSLKWAVEMNIPNIIHNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L+VP +KIGNV+RLMRYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 61 LQVPSSKIGNVRRLMRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAP 113
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN+SFN+AMASDS+
Sbjct: 114 MVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNRSFNDAMASDSK 173
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+INLAL+DC+ VF+GLESIVDVGGG GTT K+ICETFP+LKCIVFDRPQVVE+LS +NN+
Sbjct: 174 LINLALRDCDFVFDGLESIVDVGGGNGTTGKIICETFPKLKCIVFDRPQVVENLSGSNNL 233
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+P ADAVLLKYILHNWTD A NE
Sbjct: 234 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCTRILKKCKEAVTNDGKKGKVIIIDMVINE 293
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD++ ++Q+KLLMDVNMACLNG ER EEWKKLF +AGFQD+KISPLTG LS+IEIYP
Sbjct: 294 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQDYKISPLTGFLSLIEIYP 352
>sp|O22309.1|7OMT9_MEDSA RecName: Full=Isoflavone-7-O-methyltransferase 9; AltName: Full=7
IOMT-9; AltName: Full=Isoflavone-O-methyltransferase 9
gb|AAC49927.1| 7-O-methyltransferase [Medicago sativa]
Length = 352
Score = 511 bits (1317), Expect = e-179
Identities = 254/359 (70%), Positives = 293/359 (81%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGRK SEIF+ Q LLYKH+YAFIDSM LKW + ++IPNIIHNHGKPI+L LVSI
Sbjct: 1 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L+VP +KIGNV+RLMRYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 61 LQVPSSKIGNVRRLMRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAP 113
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN SFN+AMASDS+
Sbjct: 114 MVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSK 173
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+INLAL+DC+ VF+GLESIVDVGGGTGTTAK+ICETFP+LKCIVFDRPQVVE+LS +NN+
Sbjct: 174 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 233
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+P ADAVLLKYILHNWTD A NE
Sbjct: 234 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINE 293
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD++ ++Q+KLLMDVNMACLNG ER EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 294 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352
>sp|O22308.1|7OMT6_MEDSA RecName: Full=Isoflavone-7-O-methyltransferase 6; AltName:
Full=7-IOMT-6; AltName:
Full=Isoflavone-O-methyltransferase 6
gb|AAC49926.1| 7-O-methyltransferase [Medicago sativa]
Length = 352
Score = 509 bits (1311), Expect = e-178
Identities = 253/359 (70%), Positives = 293/359 (81%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGRK SEIF+ Q LLYKH+YAFIDSM LKW +E++IPNII NHGKPI+L LVSI
Sbjct: 1 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L+VP +KIGNV+RLMRYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 61 LQVPSSKIGNVRRLMRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAP 113
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN SFN+AMASDS+
Sbjct: 114 MVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSK 173
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+INLAL+DC+ VF+GLESIVDVGGGTGTTAK+ICETFP+LKCIVFDRPQVVE+LS +NN+
Sbjct: 174 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 233
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+P ADAVLLKYILHNWTD A +E
Sbjct: 234 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDE 293
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD++ ++Q+KLLMDVNMACLNG ER EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 294 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352
>sp|O24529.1|7OMT8_MEDSA RecName: Full=Isoflavone-7-O-methyltransferase 8; AltName:
Full=7-IOMT-8; AltName:
Full=Isoflavone-O-methyltransferase 8
pdb|1FP2|A Chain A, Crystal Structure Analysis Of Isoflavone O-Methyltransferase
gb|AAC49928.1| isoflavone-O-methytransferase [Medicago sativa]
Length = 352
Score = 508 bits (1307), Expect = e-177
Identities = 252/359 (70%), Positives = 293/359 (81%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGRK SEIF+ Q LLYKH+YAFIDSM LKW +E++IPNII NHGKPI+L LVSI
Sbjct: 1 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L+VP +KIGNV+RLMRYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 61 LQVPSSKIGNVRRLMRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAP 113
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN SFN+AMASDS+
Sbjct: 114 MVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSK 173
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+INLAL+DC+ VF+GLESIVDVGGGTGTTAK+ICETFP+LKCIVFDRPQVVE+LS +NN+
Sbjct: 174 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 233
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+P ADAVLLKYILHNWTD A ++
Sbjct: 234 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 293
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD++ ++Q+KLLMDVNMACLNG ER EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 294 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352
>dbj|GAU19866.1| hypothetical protein TSUD_170920 [Trifolium subterraneum]
Length = 357
Score = 498 bits (1281), Expect = e-173
Identities = 246/359 (68%), Positives = 287/359 (79%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGR SEI++ Q LLYKH+YAFIDSM LKWT+E++IPNIIHNHGKPI+L LVSI
Sbjct: 1 MASSINGRNPSEIYKAQALLYKHIYAFIDSMSLKWTVEMNIPNIIHNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L+VP +K GNVQR+MRYLAHNGFFEI+ N AYALTVASELLVKG+ LCLAP
Sbjct: 61 LQVPSSKCGNVQRIMRYLAHNGFFEIITNRESENDEE--AYALTVASELLVKGSELCLAP 118
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH++KKWIYEEDLTLFGVTLG+ F++FL NP YN+SFNEAMASDS+
Sbjct: 119 MVECVLDPTLSGSYHEMKKWIYEEDLTLFGVTLGAGFWDFLEKNPSYNESFNEAMASDSK 178
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
MINLAL+DC+ VFEGLESIVDVGGGTGTTAK+ICE FP+LKC+VFDRPQVVE+L+ ++N+
Sbjct: 179 MINLALRDCDFVFEGLESIVDVGGGTGTTAKIICERFPKLKCVVFDRPQVVENLTESDNL 238
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+P DAVLLK+ILHNW D A NE
Sbjct: 239 TYVGGDMFKSIPNGDAVLLKWILHNWIDSDCIRILKKTKEAVTSDGKKGKVIIIDMVINE 298
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
K+D++ ++Q+KLLMDVNMA LNG ER EWKKLF +AGF D+KISPLTG LS+IEIYP
Sbjct: 299 KEDENQITQVKLLMDVNMASLNGKERNENEWKKLFKEAGFHDYKISPLTGFLSLIEIYP 357
>gb|PNX99091.1| isoflavone 7-O-methyltransferase-like protein [Trifolium pratense]
Length = 358
Score = 496 bits (1276), Expect = e-173
Identities = 247/360 (68%), Positives = 290/360 (80%), Gaps = 1/360 (0%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
M+S INGRK SEI++ Q LLYKH+YAFIDSM LKWT+E++IPNIIHNHGKPI+L L+SI
Sbjct: 1 MSSLINGRKPSEIYKAQALLYKHIYAFIDSMSLKWTVEMNIPNIIHNHGKPISLSNLLSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
LKVP +K GNVQR+MRYLAHNGFFEI++ AYALTVASELLVKG+ LCLAP
Sbjct: 61 LKVPSSKCGNVQRIMRYLAHNGFFEIIKTQEFENDEE--AYALTVASELLVKGSELCLAP 118
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH++KKWIYEEDLTLFGVTLG+ F++FL NP YNKSFNEAMASDS+
Sbjct: 119 MVECVLDPTLSGSYHEMKKWIYEEDLTLFGVTLGAGFWDFLEKNPGYNKSFNEAMASDSK 178
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLS-VTNN 802
MINLAL+DC+ VFEGLESIVDVGGGTGTTAK+ICE FP+LKC+VFDRPQVVE+L+ ++N
Sbjct: 179 MINLALRDCDFVFEGLESIVDVGGGTGTTAKIICERFPKLKCVVFDRPQVVENLTDQSDN 238
Query: 803 VSCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXN 982
++ VGGDMF S+P D+VLLK+ILHNW D A N
Sbjct: 239 LTYVGGDMFQSIPNGDSVLLKWILHNWIDKDCIRILKKCKEAVTSDGKKGKVIIIDMVIN 298
Query: 983 EKKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
EKKD++ ++Q+KLLMDVNMA LNG ER +EWKKLF +AGFQD+KISPLTG LS+IEIYP
Sbjct: 299 EKKDENQITQVKLLMDVNMASLNGKERNEDEWKKLFKEAGFQDYKISPLTGYLSLIEIYP 358
>ref|XP_003621484.1| isoflavone-7-O-methyltransferase [Medicago truncatula]
pdb|2QYO|A Chain A, Crystal Structure Of Isoflavone O-methyltransferase Homolog
In Complex With Biochanin A And Sah
pdb|2QYO|B Chain B, Crystal Structure Of Isoflavone O-methyltransferase Homolog
In Complex With Biochanin A And Sah
gb|ABD83943.1| IOMT 3 [Medicago truncatula]
gb|AES77702.1| isoflavone-7-O-methyltransferase [Medicago truncatula]
Length = 357
Score = 489 bits (1258), Expect = e-170
Identities = 241/359 (67%), Positives = 283/359 (78%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS IN RK SEIF+ Q LLYK++YAF+DSM LKW+IE++IPNIIHNHGKPITL LVSI
Sbjct: 1 MASSINNRKPSEIFKAQALLYKNMYAFVDSMSLKWSIEMNIPNIIHNHGKPITLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P TK+ NVQRLMRYLAHNGFFEI+ N AYALTVASELLVKGT LCLAP
Sbjct: 61 LQIPSTKVDNVQRLMRYLAHNGFFEIITNQELENEEE--AYALTVASELLVKGTELCLAP 118
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLS S+H LKKW+YEEDLTLF V LG +EFLN NPEYN +N+A+ASDS+
Sbjct: 119 MVECVLDPTLSTSFHNLKKWVYEEDLTLFAVNLGCDLWEFLNKNPEYNTLYNDALASDSK 178
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
MINLA+KDCN VFEGLESIVDVGGG GTT K+ICETFP+L C+VFDRP+VVE+L +NN+
Sbjct: 179 MINLAMKDCNLVFEGLESIVDVGGGNGTTGKIICETFPKLTCVVFDRPKVVENLCGSNNL 238
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMFISVPKADAVLLK +LH+WTD A NE
Sbjct: 239 TYVGGDMFISVPKADAVLLKAVLHDWTDKDCIKILKKCKEAVTSDGKRGKVIVIDMVINE 298
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD++ L+Q+KLLM+V ++C+NG ER EEWKKLF +AGFQD+KISP TG++S+IEIYP
Sbjct: 299 KKDENQLTQIKLLMNVTISCVNGKERNEEEWKKLFIEAGFQDYKISPFTGLMSLIEIYP 357
>pdb|1FPX|A Chain A, Crystal Structure Analysis Of Selenomethionine Substituted
Isoflavone O-Methyltransferase
Length = 352
Score = 488 bits (1257), Expect = e-170
Identities = 245/358 (68%), Positives = 285/358 (79%)
Frame = +2
Query: 89 ASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSIL 268
AS INGRK SEIF+ Q LLYKH+YAFIDS LKW +E +IPNII NHGKPI+L LVSIL
Sbjct: 2 ASSINGRKPSEIFKAQALLYKHIYAFIDSXSLKWAVEXNIPNIIQNHGKPISLSNLVSIL 61
Query: 269 KVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAPM 448
+VP +KIGNV+RL RYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 62 QVPSSKIGNVRRLXRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAPX 114
Query: 449 VECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQM 628
VECVLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN SFN+A ASDS++
Sbjct: 115 VECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAXASDSKL 174
Query: 629 INLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNVS 808
INLAL+DC+ VF+GLESIVDVGGGTGTTAK+ICETFP+LKCIVFDRPQVVE+LS +NN++
Sbjct: 175 INLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLT 234
Query: 809 CVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNEK 988
VGGD F S+P ADAVLLKYILHNWTD A ++K
Sbjct: 235 YVGGDXFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDXVIDKK 294
Query: 989 KDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KD++ ++Q+KLL DVN ACLNG ER EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 295 KDENQVTQIKLLXDVNXACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352
>dbj|GAU19865.1| hypothetical protein TSUD_170910 [Trifolium subterraneum]
Length = 359
Score = 486 bits (1250), Expect = e-169
Identities = 238/359 (66%), Positives = 283/359 (78%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS IN RK SEIF+ Q LYKH+YA IDSM LKW+IE++IPNIIHNHGKPI+L LVSI
Sbjct: 1 MASSINNRKPSEIFKAQAQLYKHMYAHIDSMSLKWSIEMNIPNIIHNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P +KI NVQRLMRYLAHNG FEI++N AYALTV SELLVKGT LCLAP
Sbjct: 61 LQIPSSKIDNVQRLMRYLAHNGIFEIIKNQKLENEEEEEAYALTVVSELLVKGTELCLAP 120
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVEC LDPT S S+H+ KKWIYEEDLTLFGV LGS F+EFLN NP+YN FN+AMASDS+
Sbjct: 121 MVECALDPTFSSSFHKFKKWIYEEDLTLFGVNLGSDFWEFLNENPKYNTMFNDAMASDSK 180
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
MINLALKDC+ VFEGLESIVDVGGG GTTAK+IC+ FP+LKC+VFDRP+VVE+LS +N+
Sbjct: 181 MINLALKDCDFVFEGLESIVDVGGGNGTTAKIICDKFPKLKCVVFDRPKVVENLSENDNL 240
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+PKADAVLLK+ILH+WTD A NE
Sbjct: 241 TYVGGDMFTSIPKADAVLLKWILHDWTDKDCIRILKKCKEAVTSDGKRGKVIVIDMVINE 300
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KK+D+ L+++KLLM+V+++CLNG ERT +EWKKLF +AGFQD+KISP T +LS+IEI+P
Sbjct: 301 KKEDNQLTEIKLLMNVSISCLNGKERTEKEWKKLFIEAGFQDYKISPCTELLSLIEIFP 359
>pdb|6CIG|A Chain A, CRYSTAL STRUCTURE ANALYSIS OF SELENOMETHIONINE SUBSTITUTED
ISOFLAVONE O-METHYLTRANSFERASE
Length = 352
Score = 484 bits (1247), Expect = e-168
Identities = 244/358 (68%), Positives = 284/358 (79%)
Frame = +2
Query: 89 ASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSIL 268
AS INGRK SEIF+ Q LLYKH+YAFIDS LKW +E +IPNII NHGKPI+L LVSIL
Sbjct: 2 ASSINGRKPSEIFKAQALLYKHIYAFIDSXSLKWAVEXNIPNIIQNHGKPISLSNLVSIL 61
Query: 269 KVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAPM 448
+VP +KIGNV+RL RYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 62 QVPSSKIGNVRRLXRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAPX 114
Query: 449 VECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQM 628
VE VLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN SFN+A ASDS++
Sbjct: 115 VEXVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAXASDSKL 174
Query: 629 INLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNVS 808
INLAL+DC+ VF+GLESIVDVGGGTGTTAK+ICETFP+LKCIVFDRPQVVE+LS +NN++
Sbjct: 175 INLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLT 234
Query: 809 CVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNEK 988
VGGD F S+P ADAVLLKYILHNWTD A ++K
Sbjct: 235 YVGGDXFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDXVIDKK 294
Query: 989 KDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KD++ ++Q+KLL DVN ACLNG ER EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 295 KDENQVTQIKLLXDVNXACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 352
>gb|AAB88294.1| o-methytransferase [Medicago sativa]
Length = 343
Score = 484 bits (1245), Expect = e-168
Identities = 246/359 (68%), Positives = 286/359 (79%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGRK SEIF+ Q LLYKH+YAFIDSM LKW +E++IPNII NHGKPI+L LVSI
Sbjct: 1 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L+VP +KIGNV+RLMRYLAHNGFFEI+ +YALTVASELLV+G++LCLAP
Sbjct: 61 LQVPSSKIGNVRRLMRYLAHNGFFEIITKEEE-------SYALTVASELLVRGSDLCLAP 113
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLSGSYH+LKKWIYEEDLTLFGVTLGS F++FL+ NPEYN SFN+AMASDS+
Sbjct: 114 MVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSK 173
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+INLAL+DC+ VF+GLESIVDVGGGTGTTAK+ICETFP+LKCIVFDRPQVVE+LS +NN+
Sbjct: 174 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 233
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+P ADAVLLK L A +E
Sbjct: 234 TYVGGDMFTSIPNADAVLLKDCLR---------ILKKCKEAVTNDGKRGKVTIIDMVIDE 284
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD++ ++Q+KLLMDVNMACLNG ER EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 285 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP 343
>ref|NP_001241162.1| isoflavone 7-O-methyltransferase-like [Glycine max]
gb|ACU19886.1| unknown [Glycine max]
gb|KRH01300.1| hypothetical protein GLYMA_18G267900 [Glycine max]
Length = 353
Score = 480 bits (1235), Expect = e-166
Identities = 239/359 (66%), Positives = 279/359 (77%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGR ASEIF+GQ LLYKHLYAFIDSMCLK +EL IP+IIHNHG+PITL ELVSI
Sbjct: 1 MASSINGRNASEIFQGQTLLYKHLYAFIDSMCLKCIVELGIPDIIHNHGQPITLPELVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P K+ VQ LMRYLAHNGFFE VR H AYALT ASELLVK + L LAP
Sbjct: 61 LQIPPAKVSQVQSLMRYLAHNGFFERVRIHEKE------AYALTAASELLVKSSELSLAP 114
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
M+E VLDPTLS S+HQLKKW+YE+DLTLF ++LGSH ++FLN NP +NKSFNEAMASDSQ
Sbjct: 115 MIEFVLDPTLSNSFHQLKKWVYEKDLTLFDISLGSHLWDFLNKNPAHNKSFNEAMASDSQ 174
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
M+NLAL+DCN VF+GLE IVDVGGGTGTTAK+ICE FP LKCIVFDRPQV+E+LS +NN+
Sbjct: 175 MMNLALRDCNWVFQGLEFIVDVGGGTGTTAKIICEAFPNLKCIVFDRPQVIENLSGSNNL 234
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+PKAD +LLK+ILHNW D A NE
Sbjct: 235 TYVGGDMFKSIPKADVILLKWILHNWIDKDCIKILKNCKEAISNNGKRGKVIIIDVVINE 294
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
K+D+H +++LKL+MD+ MAC+NG ER EEWKKLF +AGFQD+KI PLT LS+IEIYP
Sbjct: 295 KEDEHKVTELKLVMDITMACVNGKERNEEEWKKLFMEAGFQDYKIFPLTKYLSVIEIYP 353
>gb|ABD83942.1| IOMT 2 [Medicago truncatula]
Length = 357
Score = 478 bits (1230), Expect = e-166
Identities = 237/359 (66%), Positives = 281/359 (78%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS IN RKASEIF+ Q LLYK++YAFIDSM LKW++E+DIP II+NHGKPITL LVSI
Sbjct: 1 MASSINNRKASEIFKAQALLYKNMYAFIDSMSLKWSVEMDIPTIIYNHGKPITLSNLVSI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P TK+ NVQRLMR LAHNGFFEIV N AYALTV SELLVKGT LCLAP
Sbjct: 61 LQIPSTKVDNVQRLMRLLAHNGFFEIVTNQELENEEE--AYALTVTSELLVKGTELCLAP 118
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDP + S+H KKWIYEEDLTLF +LG +EFLN NPEYN+ FN+AMASDS+
Sbjct: 119 MVECVLDPKFNVSFHNFKKWIYEEDLTLFATSLGCDLWEFLNKNPEYNRLFNDAMASDSK 178
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
MINLALKDCN VFEGL+SIVDVGGG GTT K+ICET+P+L+C+VFDRP+VVE+L +NN+
Sbjct: 179 MINLALKDCNFVFEGLDSIVDVGGGNGTTGKIICETYPKLRCVVFDRPKVVENLCGSNNL 238
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMFISVPKADAVLLK+ILH+WTD A NE
Sbjct: 239 TYVGGDMFISVPKADAVLLKWILHDWTDRDCIKILKKCKEAVTTEGKRGKVIVIDMVINE 298
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KK+++ L+Q+KLLM+V ++CLNG ER EEWKKLF +AGF+++KISP TG+LS+IEIYP
Sbjct: 299 KKEENELTQIKLLMNVTISCLNGKERNEEEWKKLFMEAGFENYKISPCTGLLSLIEIYP 357
>ref|XP_020219848.1| isoflavone 7-O-methyltransferase-like [Cajanus cajan]
gb|KYP64754.1| Isoflavone-7-O-methyltransferase 9 [Cajanus cajan]
Length = 356
Score = 476 bits (1225), Expect = e-165
Identities = 240/359 (66%), Positives = 280/359 (77%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS IN R ASE+F+GQ LLY+HLYAFIDSMCLKW +EL IP+IIHNH KPITL ELVSI
Sbjct: 1 MAS-INERSASEMFQGQALLYRHLYAFIDSMCLKWIVELRIPDIIHNHAKPITLPELVSI 59
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P TK+G VQ LMRYL+HNGFFE VR H AYALT ASELLVK ++L LAP
Sbjct: 60 LQIPPTKVGQVQSLMRYLSHNGFFERVRIHENFEEKE--AYALTAASELLVKSSDLSLAP 117
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLS SYH LKKW+++ED+TLF ++LGSHF++FL NP YN+SFN+AMASDSQ
Sbjct: 118 MVECVLDPTLSDSYHHLKKWVHQEDVTLFKISLGSHFWDFLTKNPAYNESFNDAMASDSQ 177
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+ NLAL+DC VFEGLESIVDVGGGTGTTA +I + FP LKCIVFDRPQVV +LS TNN+
Sbjct: 178 ISNLALRDCKLVFEGLESIVDVGGGTGTTATIISQAFPNLKCIVFDRPQVVHNLSGTNNL 237
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+PKADAVLLK+ILHNW+D A NE
Sbjct: 238 AFVGGDMFSSIPKADAVLLKWILHNWSDKDCVKILKNCKEAISTNGKKGKVIIIDVVINE 297
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
+D H +++LKLLMDVNMAC+NG ER+ EEWKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 298 NQDQHEVTELKLLMDVNMACVNGKERSEEEWKKLFKEAGFQGYKISPLTGYLSLIEIYP 356
>ref|XP_020219068.1| isoflavone 7-O-methyltransferase-like [Cajanus cajan]
gb|KYP64758.1| Isoflavone-7-O-methyltransferase 9 [Cajanus cajan]
Length = 356
Score = 475 bits (1223), Expect = e-165
Identities = 239/359 (66%), Positives = 279/359 (77%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGR A+E+F+GQ LLY+HLYAFIDSMCLKW +EL IP+IIHNH KPITL ELVSI
Sbjct: 1 MAS-INGRSANEMFQGQALLYRHLYAFIDSMCLKWIVELRIPDIIHNHAKPITLPELVSI 59
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P TK+G VQ LMRYL+HNGFFE VR H AYALT ASELLVK + L LAP
Sbjct: 60 LQIPPTKVGQVQSLMRYLSHNGFFERVRIHENFEEKE--AYALTAASELLVKSSELSLAP 117
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVECVLDPTLS SYH LKKW+++ED+TLF ++LGSHF++FL NP YN+SFN+AMASDSQ
Sbjct: 118 MVECVLDPTLSDSYHHLKKWVHQEDVTLFKISLGSHFWDFLTKNPAYNESFNDAMASDSQ 177
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
+ NLAL+DC VFEGLESIVDVGGGTGTTA +I + FP LKCIVFDRPQVV +LS TNN+
Sbjct: 178 ISNLALRDCKLVFEGLESIVDVGGGTGTTATIISQAFPNLKCIVFDRPQVVHNLSGTNNL 237
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+PKADAVLLK+ILHNW+D A NE
Sbjct: 238 AFVGGDMFSSIPKADAVLLKWILHNWSDKDCVKILKNCKEAISTNGKKGKVIIIDVVINE 297
Query: 986 KKDDHVLSQLKLLMDVNMACLNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
+D H +++LKLLMDVNMAC+NG ER+ EEW KLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 298 NQDQHEVTELKLLMDVNMACVNGKERSEEEWMKLFKEAGFQGYKISPLTGYLSLIEIYP 356
>ref|NP_001241010.1| uncharacterized protein LOC100812688 [Glycine max]
gb|ACU20375.1| unknown [Glycine max]
gb|KRH01299.1| hypothetical protein GLYMA_18G267800 [Glycine max]
Length = 354
Score = 469 bits (1206), Expect = e-162
Identities = 238/360 (66%), Positives = 276/360 (76%), Gaps = 1/360 (0%)
Frame = +2
Query: 86 MASPINGRKASEIFEGQVLLYKHLYAFIDSMCLKWTIELDIPNIIHNHGKPITLQELVSI 265
MAS INGR ASEIF+GQ LLYKHL+A +DS CLK +EL IP+IIHNHG+PITL EL SI
Sbjct: 1 MASSINGRNASEIFQGQTLLYKHLFAHVDSKCLKCIVELGIPDIIHNHGQPITLPELASI 60
Query: 266 LKVPQTKIGNVQRLMRYLAHNGFFEIVRNHXXXXXXXXXAYALTVASELLVKGTNLCLAP 445
L++P K+ VQ LMRYLAHNGFFE V H AYALT ASELLVK + L LAP
Sbjct: 61 LQIPPAKVSQVQSLMRYLAHNGFFERVTIHEKE------AYALTAASELLVKSSELSLAP 114
Query: 446 MVECVLDPTLSGSYHQLKKWIYEEDLTLFGVTLGSHFYEFLNGNPEYNKSFNEAMASDSQ 625
MVE +LD T+SGS+HQLKKW++EEDLTLF ++LGSH ++FLN NP YNKSFNEAMASDSQ
Sbjct: 115 MVEYILDTTISGSFHQLKKWVHEEDLTLFEISLGSHLWDFLNRNPAYNKSFNEAMASDSQ 174
Query: 626 MINLALKDCNSVFEGLESIVDVGGGTGTTAKLICETFPELKCIVFDRPQVVESLSVTNNV 805
M+NLAL+DC VFEGLESIVDVGGGTG TAK+ICE FP+LKCIVFDRPQVVE+LS +NN+
Sbjct: 175 MLNLALRDCKLVFEGLESIVDVGGGTGATAKIICEAFPDLKCIVFDRPQVVENLSGSNNL 234
Query: 806 SCVGGDMFISVPKADAVLLKYILHNWTDXXXXXXXXXXXXAXXXXXXXXXXXXXXXXXNE 985
+ VGGDMF S+PKA AVL K ILHNW+D A NE
Sbjct: 235 TYVGGDMFKSIPKACAVLFKMILHNWSDEDCRKILENCKEAISSKSKTGKVIVIDVVINE 294
Query: 986 KKDDHVLSQLKLLMDVNMAC-LNGIERTVEEWKKLFTKAGFQDHKISPLTGMLSIIEIYP 1162
KKD+H +++LKLLMD+NMAC LNG ER E+WKKLF +AGFQ +KISPLTG LS+IEIYP
Sbjct: 295 KKDEHEITRLKLLMDLNMACLLNGKERREEDWKKLFVEAGFQSYKISPLTGYLSLIEIYP 354