BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus24_contig00008640
         (1145 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012567740.1| PREDICTED: sacsin [Cicer arietinum]               666   0.0  
gb|KHM99056.1| Sacsin [Glycine soja]                                  655   0.0  
gb|KHN29572.1| Sacsin [Glycine soja]                                  661   0.0  
ref|XP_003533689.1| PREDICTED: sacsin-like [Glycine max] >gi|947...   661   0.0  
gb|KRH40865.1| hypothetical protein GLYMA_09G282200 [Glycine max]     661   0.0  
gb|KRH40867.1| hypothetical protein GLYMA_09G282200 [Glycine max]     661   0.0  
ref|XP_013466864.1| zinc finger, C3HC4 type (RING finger) protei...   656   0.0  
ref|XP_006605419.1| PREDICTED: sacsin-like [Glycine max] >gi|947...   655   0.0  
gb|PNY03759.1| zinc finger C3HC4 type (RING finger) protein, par...   652   0.0  
ref|XP_020205115.1| sacsin isoform X1 [Cajanus cajan]                 648   0.0  
ref|XP_020205116.1| sacsin isoform X2 [Cajanus cajan]                 648   0.0  
gb|KYP37061.1| Sacsin [Cajanus cajan]                                 648   0.0  
dbj|GAU44495.1| hypothetical protein TSUD_13070 [Trifolium subte...   645   0.0  
ref|XP_019420625.1| PREDICTED: sacsin isoform X1 [Lupinus angust...   634   0.0  
ref|XP_019420628.1| PREDICTED: sacsin isoform X2 [Lupinus angust...   634   0.0  
ref|XP_007153087.1| hypothetical protein PHAVU_003G005800g [Phas...   624   0.0  
ref|XP_017426833.1| PREDICTED: sacsin isoform X1 [Vigna angulari...   620   0.0  
ref|XP_017426834.1| PREDICTED: sacsin isoform X2 [Vigna angularis]    620   0.0  
ref|XP_017426835.1| PREDICTED: sacsin isoform X3 [Vigna angularis]    620   0.0  
ref|XP_020989804.1| sacsin [Arachis duranensis]                       615   0.0  

>ref|XP_012567740.1| PREDICTED: sacsin [Cicer arietinum]
          Length = 4748

 Score =  666 bits (1718), Expect = 0.0
 Identities = 327/369 (88%), Positives = 345/369 (93%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAKKLHLIYDKREHPRQSLLQHNLGE+QGPALVAIFE A LSREE SNFQLLPPWR
Sbjct: 2822 ADCCKAKKLHLIYDKREHPRQSLLQHNLGEYQGPALVAIFEGACLSREEFSNFQLLPPWR 2881

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLV CYSICD+LSVVSGGYFYMFDPRGL LAALSTN P+AKMFSL GT+L
Sbjct: 2882 LRGNTLNYGLGLVSCYSICDVLSVVSGGYFYMFDPRGLVLAALSTNAPSAKMFSLIGTDL 2941

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
             QRF DQFSPMLIDQN+LWSLSDSTIIRMPLS +CLKV SDFG+N+IKHI+DIF+E GSR
Sbjct: 2942 KQRFHDQFSPMLIDQNDLWSLSDSTIIRMPLSSDCLKVGSDFGTNQIKHITDIFMEHGSR 3001

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVLEVSISTWEEG SHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN
Sbjct: 3002 ALLFLKSVLEVSISTWEEGQSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 3061

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AA+KM VIDV+LC EGTT IDRWLLVL+LGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 3062 AAIKMPVIDVSLCLEGTTFIDRWLLVLTLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 3121

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH +NIYS SSIMTPLPLSG I+LPVT+ GCFLVCHN GRYLFKYQD+GASA GHFD G
Sbjct: 3122 NGHHSNIYSMSSIMTPLPLSGRINLPVTIFGCFLVCHNRGRYLFKYQDKGASAEGHFDVG 3181

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3182 NQLIESWNR 3190



 Score =  108 bits (269), Expect = 1e-21
 Identities = 111/387 (28%), Positives = 166/387 (42%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D R H   SLL  +L ++QGPAL+A +  A  S E+  +   +    
Sbjct: 45   ADDAGATTVSLCLDLRSHGSDSLLSDSLSQWQGPALLA-YNDAVFSEEDFVSISKIGGSS 103

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K      
Sbjct: 104  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSAANP-GKRIDFTS 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI---KHISDIF 559
            ++    ++DQFSP      ++ S    T+ R PL  N  +  S   S +    + IS +F
Sbjct: 163  SSALSFYKDQFSPFCAFGCDMQSPFAGTLFRFPLR-NVEQAASSKLSRQAYSPEDISSMF 221

Query: 560  I---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQ 730
            +   E+G   LLFLKSVL + +  W+ G   P +  S S+   SS+      +  W +  
Sbjct: 222  VQLFEEGILTLLFLKSVLCIEMYVWDGGEPEPKKINSCSV---SSVT----DDTVWHRQA 274

Query: 731  LSRLFSSSNAAMKMHVIDVTLCSEGTT------IIDRWLLVLSLGSGQTRNMALDR---R 883
            L RL    N   ++    +   SE  +        +R+ +V ++ S  +R  +  +   +
Sbjct: 275  LLRLSKCLNTTTEVDAFPLDFVSEAISGAETERHTERFYVVQTMASASSRIGSFAKTASK 334

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
                +L P A IAA IS N  P  + +       LPL     L V V G F V  N    
Sbjct: 335  EYDIHLMPWASIAACISDNS-PNKVLTTGLAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 390

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++   WNR
Sbjct: 391  ------RGIWYGDDMDRSGKVRSIWNR 411



 Score = 64.7 bits (156), Expect = 2e-07
 Identities = 81/325 (24%), Positives = 137/325 (42%), Gaps = 26/325 (8%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1412 AEDAGASEVIFLLDKSQYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1471

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    +FDP    L  +S + P  ++    G 
Sbjct: 1472 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVLFDPHASNLPGISPSHPGLRI-KFVGR 1530

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFS +L    +L +    T+ R PL      S + +K E  +    ++ +  
Sbjct: 1531 QILEQFPDQFSSLLHFGCDLQNPFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 1589

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH-SHPCQNFSISIDPSSSI----MRNPFS 706
             F E  S  LLFL +V  +SI     T +E H  H  +   I      S     + N F 
Sbjct: 1590 AFSEVVSETLLFLHNVKSISIFVKEGTGDEMHLLHRVRRNCIGEPEIGSTEAQDVFNFFK 1649

Query: 707  EKK---WRKFQLSRLFSSS---NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTR-- 862
            + +     + Q  +  S S   +   K   I +T  S     +  W++   LG G  R  
Sbjct: 1650 QNRRVGMNRAQFLKKLSQSIDRDLPYKCQKILITEKSISGHHLHYWMITECLGGGNARKG 1709

Query: 863  -NMALDRRYLAYNLTPVAGIAALIS 934
             + A +R    YN  P A +AA ++
Sbjct: 1710 TSEAANRN--CYNFVPWACVAAYLN 1732


>gb|KHM99056.1| Sacsin [Glycine soja]
          Length = 2398

 Score =  655 bits (1690), Expect = 0.0
 Identities = 316/369 (85%), Positives = 340/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLGEFQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 462  ADCCKAKRLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFECACLSREEFSNFQLRPPWR 521

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDPRGL L A STN P+AKMFSL GT+L
Sbjct: 522  LRGNTINYGLGLVCCYSICDLLSVISGGYFYMFDPRGLVLGAPSTNAPSAKMFSLIGTDL 581

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKVE D GSNRIKHI+DIF+E GSR
Sbjct: 582  TQRFCDQFSPMLIDRNDLWSLADSTIIRMPLSSDCLKVEPDLGSNRIKHITDIFMEHGSR 641

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHP +NFSISIDPSSSI+RNPFSEKKWR FQLSR+FSSSN
Sbjct: 642  ALLFLKSVLQVSISTWEEGHSHPSKNFSISIDPSSSILRNPFSEKKWRNFQLSRIFSSSN 701

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMH IDV L SEGTT+IDRWL+ LSLGSGQTRNMALDRRYLAY+LTPVAGIAALIS 
Sbjct: 702  AVIKMHAIDVNLYSEGTTVIDRWLVALSLGSGQTRNMALDRRYLAYDLTPVAGIAALISS 761

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLP+SGCI++P+TVLGCFLVCHN GRYLFKYQDRG  A GHFDAG
Sbjct: 762  NGHHANVYSRSSIMAPLPMSGCINMPITVLGCFLVCHNRGRYLFKYQDRGTLAEGHFDAG 821

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 822  NQLIESWNR 830


>gb|KHN29572.1| Sacsin [Glycine soja]
          Length = 3507

 Score =  661 bits (1705), Expect = 0.0
 Identities = 319/369 (86%), Positives = 342/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLG+FQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 1570 ADCCKAKRLHLIYDKREHPRQSLLQHNLGDFQGPALVAIFEGACLSREEFSNFQLRPPWR 1629

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDPRGL L   STN P+AKMFSL GT+L
Sbjct: 1630 LRGNTINYGLGLVCCYSICDLLSVISGGYFYMFDPRGLVLGVPSTNAPSAKMFSLIGTDL 1689

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKVE   GSNRIKHI+DIF+E GSR
Sbjct: 1690 TQRFCDQFSPMLIDRNDLWSLTDSTIIRMPLSSDCLKVEPGLGSNRIKHITDIFMEHGSR 1749

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHP QNFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 1750 ALLFLKSVLQVSISTWEEGHSHPSQNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 1809

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+VL LGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 1810 AVIKMHVIDVNLYSEGTTVIDRWLVVLCLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 1869

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLPLSGCI++P+T+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 1870 NGHHANVYSRSSIMAPLPLSGCINMPITILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 1929

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 1930 NQLIESWNR 1938



 Score = 69.3 bits (168), Expect = 7e-09
 Identities = 79/323 (24%), Positives = 134/323 (41%), Gaps = 24/323 (7%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 159  AEDAGASEVIFLLDKSHYGTSSILSPEMADWQGPALYCYNDSVFSPQDLYAISRIGQESK 218

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 219  LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENVVMFDPHASNLPGISPSHPGLRI-KFVGR 277

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E+ +    ++ +  
Sbjct: 278  QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEA-YTPEDVRSLFA 336

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGHSHPCQNFSISIDP-----SSSIMRNPFS 706
             F E  S  LLFL++V  +SI     T  E H   C   +   +P      +  + N F 
Sbjct: 337  AFSEVVSETLLFLRNVKSISIFVKEGTGHEMHLLHCVRRTCIGEPEFGSTEAQDVFNFFK 396

Query: 707  EKK---WRKFQLSRLFSSS---NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNM 868
            E +     + Q  +  S S   +   K   + +T  S  +     W+    LG G  +  
Sbjct: 397  ESRHVGMNRVQFLKKLSLSIGRDLPYKCQKMLITEQSTSSCNSHYWITTECLGDGNAQKR 456

Query: 869  ALD-RRYLAYNLTPVAGIAALIS 934
              +      YN  P A +AA ++
Sbjct: 457  TSETANSNCYNFVPWACVAAYLN 479


>ref|XP_003533689.1| PREDICTED: sacsin-like [Glycine max]
 gb|KRH40866.1| hypothetical protein GLYMA_09G282200 [Glycine max]
          Length = 4760

 Score =  661 bits (1705), Expect = 0.0
 Identities = 319/369 (86%), Positives = 342/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLG+FQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 2823 ADCCKAKRLHLIYDKREHPRQSLLQHNLGDFQGPALVAIFEGACLSREEFSNFQLRPPWR 2882

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDPRGL L   STN P+AKMFSL GT+L
Sbjct: 2883 LRGNTINYGLGLVCCYSICDLLSVISGGYFYMFDPRGLVLGVPSTNAPSAKMFSLIGTDL 2942

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKVE   GSNRIKHI+DIF+E GSR
Sbjct: 2943 TQRFCDQFSPMLIDRNDLWSLTDSTIIRMPLSSDCLKVEPGLGSNRIKHITDIFMEHGSR 3002

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHP QNFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 3003 ALLFLKSVLQVSISTWEEGHSHPSQNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 3062

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+VL LGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 3063 AVIKMHVIDVNLYSEGTTVIDRWLVVLCLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 3122

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLPLSGCI++P+T+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 3123 NGHHANVYSRSSIMAPLPLSGCINMPITILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 3182

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3183 NQLIESWNR 3191



 Score =  117 bits (293), Expect = 1e-24
 Identities = 114/388 (29%), Positives = 167/388 (43%), Gaps = 19/388 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL  +L ++QGPAL+A F  A  + E+  +   +    
Sbjct: 44   ADDAGATTVSLCLDRRSHAAGSLLSDSLAQWQGPALLA-FNDAVFTEEDFVSISKIGGST 102

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K     G
Sbjct: 103  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSAANP-GKRIDFTG 161

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI---KHISDIF 559
            ++    +RDQFSP      ++ S    T+ R PL  N  +  +   S +    + IS +F
Sbjct: 162  SSALSFYRDQFSPYCAFGCDMQSPFSGTLFRFPLR-NAYQAATSKLSRQAYSPEDISSMF 220

Query: 560  I---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQ 730
            +   E+G   LLFLKSVL + +  W+ G   P +  S S+   SS+      +  W +  
Sbjct: 221  VQLYEEGVLTLLFLKSVLCIEMYLWDAGEPEPKKIHSCSV---SSVT----DDTVWHRQA 273

Query: 731  LSRLFSSSNAAMKMHVIDVTLC------SEGTTIIDRWLLVLSLGSGQTR----NMALDR 880
            L RL  S N   ++    +          E     +R+ +V ++ S  +R      +  +
Sbjct: 274  LLRLSKSLNTTAEVDAFPLDFLIERINGDESERQKERFYVVQTMASASSRIGSFASSASK 333

Query: 881  RYLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGR 1060
             Y   +L P A IAA IS N    NI         LPL     L V V G F V  N   
Sbjct: 334  EY-DIHLLPWASIAACISDNSQNNNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR-- 390

Query: 1061 YLFKYQDRGASAGGHFDAGIQLIESWNR 1144
                   RG   G   D   ++  +WNR
Sbjct: 391  -------RGIWYGDDMDRSGKVRSTWNR 411



 Score = 68.9 bits (167), Expect = 1e-08
 Identities = 58/210 (27%), Positives = 97/210 (46%), Gaps = 8/210 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1412 AEDAGASEVIFLLDKSHYGTSSILSPEMADWQGPALYCYNDSVFSPQDLYAISRIGQESK 1471

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1472 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENVVMFDPHASNLPGISPSHPGLRI-KFVGR 1530

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E+ +    ++ +  
Sbjct: 1531 QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEA-YTPEDVRSLFA 1589

Query: 554  IFIEQGSRALLFLKSVLEVSISTWEEGHSH 643
             F E  S  LLFL++V  +SI   +EG  H
Sbjct: 1590 AFSEVVSETLLFLRNVKSISIFV-KEGTGH 1618


>gb|KRH40865.1| hypothetical protein GLYMA_09G282200 [Glycine max]
          Length = 4758

 Score =  661 bits (1705), Expect = 0.0
 Identities = 319/369 (86%), Positives = 342/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLG+FQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 2823 ADCCKAKRLHLIYDKREHPRQSLLQHNLGDFQGPALVAIFEGACLSREEFSNFQLRPPWR 2882

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDPRGL L   STN P+AKMFSL GT+L
Sbjct: 2883 LRGNTINYGLGLVCCYSICDLLSVISGGYFYMFDPRGLVLGVPSTNAPSAKMFSLIGTDL 2942

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKVE   GSNRIKHI+DIF+E GSR
Sbjct: 2943 TQRFCDQFSPMLIDRNDLWSLTDSTIIRMPLSSDCLKVEPGLGSNRIKHITDIFMEHGSR 3002

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHP QNFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 3003 ALLFLKSVLQVSISTWEEGHSHPSQNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 3062

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+VL LGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 3063 AVIKMHVIDVNLYSEGTTVIDRWLVVLCLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 3122

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLPLSGCI++P+T+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 3123 NGHHANVYSRSSIMAPLPLSGCINMPITILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 3182

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3183 NQLIESWNR 3191



 Score =  117 bits (293), Expect = 1e-24
 Identities = 114/388 (29%), Positives = 167/388 (43%), Gaps = 19/388 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL  +L ++QGPAL+A F  A  + E+  +   +    
Sbjct: 44   ADDAGATTVSLCLDRRSHAAGSLLSDSLAQWQGPALLA-FNDAVFTEEDFVSISKIGGST 102

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K     G
Sbjct: 103  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSAANP-GKRIDFTG 161

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI---KHISDIF 559
            ++    +RDQFSP      ++ S    T+ R PL  N  +  +   S +    + IS +F
Sbjct: 162  SSALSFYRDQFSPYCAFGCDMQSPFSGTLFRFPLR-NAYQAATSKLSRQAYSPEDISSMF 220

Query: 560  I---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQ 730
            +   E+G   LLFLKSVL + +  W+ G   P +  S S+   SS+      +  W +  
Sbjct: 221  VQLYEEGVLTLLFLKSVLCIEMYLWDAGEPEPKKIHSCSV---SSVT----DDTVWHRQA 273

Query: 731  LSRLFSSSNAAMKMHVIDVTLC------SEGTTIIDRWLLVLSLGSGQTR----NMALDR 880
            L RL  S N   ++    +          E     +R+ +V ++ S  +R      +  +
Sbjct: 274  LLRLSKSLNTTAEVDAFPLDFLIERINGDESERQKERFYVVQTMASASSRIGSFASSASK 333

Query: 881  RYLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGR 1060
             Y   +L P A IAA IS N    NI         LPL     L V V G F V  N   
Sbjct: 334  EY-DIHLLPWASIAACISDNSQNNNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR-- 390

Query: 1061 YLFKYQDRGASAGGHFDAGIQLIESWNR 1144
                   RG   G   D   ++  +WNR
Sbjct: 391  -------RGIWYGDDMDRSGKVRSTWNR 411



 Score = 68.9 bits (167), Expect = 1e-08
 Identities = 58/210 (27%), Positives = 97/210 (46%), Gaps = 8/210 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1412 AEDAGASEVIFLLDKSHYGTSSILSPEMADWQGPALYCYNDSVFSPQDLYAISRIGQESK 1471

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1472 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENVVMFDPHASNLPGISPSHPGLRI-KFVGR 1530

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E+ +    ++ +  
Sbjct: 1531 QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEA-YTPEDVRSLFA 1589

Query: 554  IFIEQGSRALLFLKSVLEVSISTWEEGHSH 643
             F E  S  LLFL++V  +SI   +EG  H
Sbjct: 1590 AFSEVVSETLLFLRNVKSISIFV-KEGTGH 1618


>gb|KRH40867.1| hypothetical protein GLYMA_09G282200 [Glycine max]
          Length = 4579

 Score =  661 bits (1705), Expect = 0.0
 Identities = 319/369 (86%), Positives = 342/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLG+FQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 2642 ADCCKAKRLHLIYDKREHPRQSLLQHNLGDFQGPALVAIFEGACLSREEFSNFQLRPPWR 2701

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDPRGL L   STN P+AKMFSL GT+L
Sbjct: 2702 LRGNTINYGLGLVCCYSICDLLSVISGGYFYMFDPRGLVLGVPSTNAPSAKMFSLIGTDL 2761

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKVE   GSNRIKHI+DIF+E GSR
Sbjct: 2762 TQRFCDQFSPMLIDRNDLWSLTDSTIIRMPLSSDCLKVEPGLGSNRIKHITDIFMEHGSR 2821

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHP QNFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 2822 ALLFLKSVLQVSISTWEEGHSHPSQNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 2881

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+VL LGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 2882 AVIKMHVIDVNLYSEGTTVIDRWLVVLCLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 2941

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLPLSGCI++P+T+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 2942 NGHHANVYSRSSIMAPLPLSGCINMPITILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 3001

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3002 NQLIESWNR 3010



 Score = 68.9 bits (167), Expect = 1e-08
 Identities = 58/210 (27%), Positives = 97/210 (46%), Gaps = 8/210 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1231 AEDAGASEVIFLLDKSHYGTSSILSPEMADWQGPALYCYNDSVFSPQDLYAISRIGQESK 1290

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1291 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENVVMFDPHASNLPGISPSHPGLRI-KFVGR 1349

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E+ +    ++ +  
Sbjct: 1350 QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEA-YTPEDVRSLFA 1408

Query: 554  IFIEQGSRALLFLKSVLEVSISTWEEGHSH 643
             F E  S  LLFL++V  +SI   +EG  H
Sbjct: 1409 AFSEVVSETLLFLRNVKSISIFV-KEGTGH 1437


>ref|XP_013466864.1| zinc finger, C3HC4 type (RING finger) protein [Medicago truncatula]
 gb|KEH40905.1| zinc finger, C3HC4 type (RING finger) protein [Medicago truncatula]
          Length = 4760

 Score =  656 bits (1692), Expect = 0.0
 Identities = 323/369 (87%), Positives = 341/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC AKKLHLIYDKREHPRQSLLQHNLGEFQGP+LVAIFE A LSREE SNFQLLPPWR
Sbjct: 2822 ADCCNAKKLHLIYDKREHPRQSLLQHNLGEFQGPSLVAIFEGACLSREEFSNFQLLPPWR 2881

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLV CYSICDLLSVVSGGYFYMFDPRGL LAALSTN P+AKMFSL GT+L
Sbjct: 2882 LRGNTINYGLGLVSCYSICDLLSVVSGGYFYMFDPRGLVLAALSTNAPSAKMFSLIGTDL 2941

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
             QRF DQFSPM ID+N+LWSLSDSTIIRMPLS +CLKV SD G+NRIK+I+DIF+E GSR
Sbjct: 2942 KQRFHDQFSPMFIDRNDLWSLSDSTIIRMPLSSDCLKVGSDLGTNRIKNITDIFMEHGSR 3001

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVLEVSISTWEEG SHPC+NFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN
Sbjct: 3002 ALLFLKSVLEVSISTWEEGQSHPCKNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 3061

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AA+KMHVIDV LCSEGT  IDRWLLVL+LGSGQTRNMALDRRYLAYNLTP+AGIAALISR
Sbjct: 3062 AAIKMHVIDVNLCSEGTKFIDRWLLVLTLGSGQTRNMALDRRYLAYNLTPIAGIAALISR 3121

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG+  NIYS SSIMTPLPLSG I LPVTV GCFLVCHN GRYLFKYQD+GASA G+FDAG
Sbjct: 3122 NGNHMNIYSTSSIMTPLPLSGHIKLPVTVFGCFLVCHNRGRYLFKYQDKGASAEGNFDAG 3181

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3182 NQLIESWNR 3190



 Score =  114 bits (286), Expect = 8e-24
 Identities = 110/388 (28%), Positives = 167/388 (43%), Gaps = 19/388 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H R+SLL  +L ++QGPAL+A +  A  S E+  +   +    
Sbjct: 45   ADDAGATTVSLCLDRRSHGRESLLSDSLSQWQGPALLA-YNDAVFSEEDFVSISKIGGSS 103

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K      
Sbjct: 104  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYLVLFDPQGVYLPRVSAANP-GKRIDFTS 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI---KHISDIF 559
            ++    ++DQF P      ++ S    T+ R PL  N  +  S   S +    + IS +F
Sbjct: 163  SSALSFYKDQFFPFCAFGCDMQSSFTGTLFRFPLR-NADQAASSKLSRQAYSPEDISSMF 221

Query: 560  I---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQ 730
            +   E+G   LLFLKSVL + +  W +G + P +  S S+   SS+      +  W +  
Sbjct: 222  VQLFEEGILTLLFLKSVLCIEMYVWNDGEAEPKKIHSCSV---SSVT----DDTVWHRQA 274

Query: 731  LSRLFSSSNAAMKMHVIDVTLCSEGTTII------DRWLLVLSLGSGQTR----NMALDR 880
            L RL    N   +M    +   SE  + +      +R+ +V ++ S  +R         +
Sbjct: 275  LLRLSKCPNTTTEMDAFPLNFVSETISGVETERQTERFYVVQTMASASSRIGSFATTASK 334

Query: 881  RYLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGR 1060
             Y   +L P A +AA IS       +         LPL     L V V G F V  N   
Sbjct: 335  EY-DIHLMPWASVAACISDQSPNNKVLRTGQAFCFLPLPVRTGLSVQVNGYFEVSSNR-- 391

Query: 1061 YLFKYQDRGASAGGHFDAGIQLIESWNR 1144
                   RG   G   D   ++  +WNR
Sbjct: 392  -------RGIWYGDDMDRSGKVRSNWNR 412



 Score = 65.9 bits (159), Expect = 9e-08
 Identities = 56/205 (27%), Positives = 93/205 (45%), Gaps = 8/205 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1414 AEDAGASEVIFLLDKSQYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1473

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1474 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1532

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFS ML    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1533 QILEQFPDQFSSMLHFGCDLQEPFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 1591

Query: 554  IFIEQGSRALLFLKSVLEVSISTWE 628
             F E  S  LL+L +V  +SI T E
Sbjct: 1592 AFSEVVSETLLYLHNVKSISIFTKE 1616


>ref|XP_006605419.1| PREDICTED: sacsin-like [Glycine max]
 gb|KRG89136.1| hypothetical protein GLYMA_20G003400 [Glycine max]
          Length = 4758

 Score =  655 bits (1690), Expect = 0.0
 Identities = 316/369 (85%), Positives = 340/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLGEFQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 2822 ADCCKAKRLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFECACLSREEFSNFQLRPPWR 2881

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDPRGL L A STN P+AKMFSL GT+L
Sbjct: 2882 LRGNTINYGLGLVCCYSICDLLSVISGGYFYMFDPRGLVLGAPSTNAPSAKMFSLIGTDL 2941

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKVE D GSNRIKHI+DIF+E GSR
Sbjct: 2942 TQRFCDQFSPMLIDRNDLWSLADSTIIRMPLSSDCLKVEPDLGSNRIKHITDIFMEHGSR 3001

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHP +NFSISIDPSSSI+RNPFSEKKWR FQLSR+FSSSN
Sbjct: 3002 ALLFLKSVLQVSISTWEEGHSHPSKNFSISIDPSSSILRNPFSEKKWRNFQLSRIFSSSN 3061

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMH IDV L SEGTT+IDRWL+ LSLGSGQTRNMALDRRYLAY+LTPVAGIAALIS 
Sbjct: 3062 AVIKMHAIDVNLYSEGTTVIDRWLVALSLGSGQTRNMALDRRYLAYDLTPVAGIAALISS 3121

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLP+SGCI++P+TVLGCFLVCHN GRYLFKYQDRG  A GHFDAG
Sbjct: 3122 NGHHANVYSRSSIMAPLPMSGCINMPITVLGCFLVCHNRGRYLFKYQDRGTLAEGHFDAG 3181

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3182 NQLIESWNR 3190



 Score =  114 bits (286), Expect = 8e-24
 Identities = 112/386 (29%), Positives = 167/386 (43%), Gaps = 17/386 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R HP  SLL  +L ++QGPAL+A F  A  + E+  +   +    
Sbjct: 44   ADDAGATTVSLCLDRRSHPAGSLLSDSLAQWQGPALLA-FNDAVFTEEDFVSISKIGGST 102

Query: 218  LRG---NTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VS  Y  +FDP+G+ L  +S   P  K     G
Sbjct: 103  KHGQASKTGRFGVGFNSVYHLTDLPSFVSHKYVVLFDPQGVYLPRVSAANP-GKRIDFTG 161

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL--SPNCLKVESDFGSNRIKHISDIFI 562
            ++    +RDQFSP      ++ S    T+ R PL  +    K +    +   + IS +F+
Sbjct: 162  SSAFSFYRDQFSPYCAFGCDMQSPFSGTLFRFPLRNADQAAKSKLSRQAYSPEDISSMFV 221

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + +  W+ G   P +  S S+   SS+      +  W +  L
Sbjct: 222  QLFEEGVLTLLFLKSVLCIEMYLWDAGEPEPKKIHSCSV---SSVT----DDTVWHRQSL 274

Query: 734  SRLFSSSNAAMKM------HVIDVTLCSEGTTIIDRWLLVLSLGSGQTR---NMALDRRY 886
             RL  S N   ++       +I+     E     +R+ +V ++ S  +R     +   + 
Sbjct: 275  LRLSKSLNTIAEVDAFPLDFLIERISGDEAERQTERFYVVQTMASTSSRIGSFASTASKE 334

Query: 887  LAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYL 1066
               +L P A +AA IS N    NI         LPL     L V V G F V  N     
Sbjct: 335  YDIHLLPWASVAACISDNFLNNNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR---- 390

Query: 1067 FKYQDRGASAGGHFDAGIQLIESWNR 1144
                 RG   G   D   ++  +WNR
Sbjct: 391  -----RGIWYGDDMDRSGKVRSTWNR 411



 Score = 67.8 bits (164), Expect = 2e-08
 Identities = 55/201 (27%), Positives = 93/201 (46%), Gaps = 8/201 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1411 AEDAGASEVMFLLDKSHYGTSSVLSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1470

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1471 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENVVMFDPHASNLPGISPSHPGLRI-KFVGQ 1529

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E+ +    ++ +  
Sbjct: 1530 QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGLASRSQIKKEA-YTPEDVRSLLA 1588

Query: 554  IFIEQGSRALLFLKSVLEVSI 616
             F E  S  LLFL++V  +SI
Sbjct: 1589 AFSEVVSETLLFLRNVKSISI 1609


>gb|PNY03759.1| zinc finger C3HC4 type (RING finger) protein, partial [Trifolium
            pratense]
          Length = 4410

 Score =  652 bits (1681), Expect = 0.0
 Identities = 317/369 (85%), Positives = 340/369 (92%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC+AKKLHLIYDKREHPRQSLLQHNLGE+QGP LVAIFE A LSREE SNFQLLPPWR
Sbjct: 2482 ADCCQAKKLHLIYDKREHPRQSLLQHNLGEYQGPGLVAIFEGACLSREEFSNFQLLPPWR 2541

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+N GLGLVCCYSICDLLSVVSGGYFYMFDPRGL LAALSTN P+AKMFSL GT+L
Sbjct: 2542 LRGNTLNNGLGLVCCYSICDLLSVVSGGYFYMFDPRGLVLAALSTNAPSAKMFSLTGTDL 2601

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
             QRF DQFSPMLID N+LWS SDSTIIRMPLS + LK+ SD G+NRIKHI+DIF+E GSR
Sbjct: 2602 KQRFHDQFSPMLIDHNDLWSSSDSTIIRMPLSSDSLKIGSDLGTNRIKHITDIFMEHGSR 2661

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVLEVSISTWEEG SHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSS+
Sbjct: 2662 ALLFLKSVLEVSISTWEEGQSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSH 2721

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AA+KMHVIDV++CSEGTT +D+WLLVL+LGSGQTRNMALDRRYLAYNLTP+AGIAALISR
Sbjct: 2722 AAIKMHVIDVSVCSEGTTFVDQWLLVLTLGSGQTRNMALDRRYLAYNLTPIAGIAALISR 2781

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH  NIY   SIMTPLPLSG I+LPVTV GCFLVCHN GRYLFKYQD+GASA G++DAG
Sbjct: 2782 NGHHTNIYPTGSIMTPLPLSGHINLPVTVFGCFLVCHNRGRYLFKYQDKGASAAGNYDAG 2841

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 2842 NQLIESWNR 2850



 Score = 66.2 bits (160), Expect = 7e-08
 Identities = 82/326 (25%), Positives = 134/326 (41%), Gaps = 27/326 (8%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1071 AEDAGASEVIFLLDKSQYGTSSVLSPEMADWQGPALYCFNDSIFSPQDLYAISRIGQESK 1130

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1131 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1189

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFS ML    +L      TI R PL      S + +K E  +    ++ +  
Sbjct: 1190 QILEQFPDQFSSMLHFGCDLQQSFPGTIFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 1248

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH-SHPCQNFSISIDPSSSI----MRNPFS 706
             F E  S  LLFL +V  +SI     T +E H  H  +   I      S     + N F 
Sbjct: 1249 AFSEVVSETLLFLHNVKSISIFVKEGTGDEMHLLHRVRRTCIGEPEIGSTKAQDVFNFFK 1308

Query: 707  EKK---WRKFQLSRLFSSS------NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQT 859
            E +     + Q  +  S S          KM + + ++ S     +  W++   LG G  
Sbjct: 1309 ENRRVGMNRVQFLKNLSQSIGRDLPYKCQKMLITEQSISSGHH--LHYWIITECLGGGNV 1366

Query: 860  RNMALD-RRYLAYNLTPVAGIAALIS 934
            R    +      YN  P A +AA ++
Sbjct: 1367 RKSTSEASNSNCYNFVPWACVAAYLN 1392


>ref|XP_020205115.1| sacsin isoform X1 [Cajanus cajan]
          Length = 4748

 Score =  648 bits (1671), Expect = 0.0
 Identities = 315/369 (85%), Positives = 337/369 (91%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLGEFQGPAL+AIFE A LSREE SNFQL PPWR
Sbjct: 2823 ADCCKAKRLHLIYDKREHPRQSLLQHNLGEFQGPALIAIFEGACLSREEFSNFQLRPPWR 2882

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICD+LSV+SGGYFYMFDPRGL L A STN P+AKMFSL GT+L
Sbjct: 2883 LRGNTINYGLGLVCCYSICDILSVISGGYFYMFDPRGLVLGAPSTNGPSAKMFSLIGTDL 2942

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
              RF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKV  D  SNRIKHI DIF+E GSR
Sbjct: 2943 KHRFCDQFSPMLIDRNDLWSLADSTIIRMPLSSDCLKVGPDLESNRIKHIIDIFMEHGSR 3002

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHPCQNFSISIDPSSSI+RNPF EKKWRKFQLSR+FSSSN
Sbjct: 3003 ALLFLKSVLQVSISTWEEGHSHPCQNFSISIDPSSSILRNPFLEKKWRKFQLSRIFSSSN 3062

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT++DRWL+VLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 3063 AVIKMHVIDVNLYSEGTTVMDRWLVVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 3122

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG  AN+YS SSIM PLPLSG I +PVT+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 3123 NGDHANVYSMSSIMAPLPLSGSIKMPVTILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 3182

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3183 NQLIESWNR 3191



 Score =  115 bits (287), Expect = 6e-24
 Identities = 110/387 (28%), Positives = 168/387 (43%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL ++L ++QGPAL+A +  A  + ++  +   +    
Sbjct: 45   ADDAGATTVSLCLDRRSHGGDSLLSNSLAQWQGPALLA-YNDAVFTEDDFVSISKIGGSS 103

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S + P  K     G
Sbjct: 104  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSASNP-GKRIDFAG 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL--SPNCLKVESDFGSNRIKHISDIFI 562
            ++    +RDQFSP       + S    T+ R PL  +    K +    +   + IS +F+
Sbjct: 163  SSALSFYRDQFSPYCAFGCNMQSPFSGTLFRFPLRNADQAAKSKLSRQAYSPEDISSMFV 222

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + +  W+ G   P + +S S+   SS+      +  W +  L
Sbjct: 223  QLFEEGVLTLLFLKSVLCIEMYLWDVGEPEPKKIYSCSV---SSVT----DDTVWHRQAL 275

Query: 734  SRLFSSSNAAMKMHVIDVTLCS------EGTTIIDRWLLVLSLGSGQTR----NMALDRR 883
             RL    N+  ++    +   S      E     +++ +V ++ S  +R         + 
Sbjct: 276  LRLSKCLNSTSEVDAFPLDFLSERINGDEAERQTEKFYVVQTMASMSSRIGSFATTASKE 335

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
            Y   +L P A IAA IS N   +NI         LPL     L V V G F V  N    
Sbjct: 336  Y-DIHLLPWASIAACISDNSLNSNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 391

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++   WNR
Sbjct: 392  ------RGIWYGDDMDRSGKVRSIWNR 412



 Score = 66.6 bits (161), Expect = 5e-08
 Identities = 81/337 (24%), Positives = 134/337 (39%), Gaps = 24/337 (7%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1413 AEDAGASEVIFLLDKSHYGTSSILSPEMADWQGPALYCFNDSIFSPQDLYAISRIGQESK 1472

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    +FDP    L  +S + P  ++    G 
Sbjct: 1473 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVLFDPHASNLPGISPSHPGLRI-KFVGR 1531

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1532 QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 1590

Query: 554  IFIEQGSRALLFLKSVLEVSISTWE-EGH--------SHPCQNFSISIDPSSSIMRNPFS 706
             F E  S  LLFL +V  +SI   E  GH        S  C          +  + N F 
Sbjct: 1591 TFSEVVSETLLFLHNVKSISIFVKEGTGHEMHLLHRVSRTCIGEPEIGSTEAQDVFNFFK 1650

Query: 707  EKK---WRKFQLSRLFSSS---NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNM 868
            E +     + Q  +  S S   +   K   I +T  S  +     W+    LG G  +  
Sbjct: 1651 ESRHVGMNRVQFLKKISLSIGRDLPYKCQKILITEQSTSSRNSHYWITTECLGDGNAQKR 1710

Query: 869  ALDR-RYLAYNLTPVAGIAALISRNGHPANIYSRSSI 976
              +      YN  P A +AA ++      N+   S +
Sbjct: 1711 TSETVNSNCYNFVPWACVAAYLNSVKVDGNLVESSEV 1747


>ref|XP_020205116.1| sacsin isoform X2 [Cajanus cajan]
          Length = 4746

 Score =  648 bits (1671), Expect = 0.0
 Identities = 315/369 (85%), Positives = 337/369 (91%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLGEFQGPAL+AIFE A LSREE SNFQL PPWR
Sbjct: 2823 ADCCKAKRLHLIYDKREHPRQSLLQHNLGEFQGPALIAIFEGACLSREEFSNFQLRPPWR 2882

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICD+LSV+SGGYFYMFDPRGL L A STN P+AKMFSL GT+L
Sbjct: 2883 LRGNTINYGLGLVCCYSICDILSVISGGYFYMFDPRGLVLGAPSTNGPSAKMFSLIGTDL 2942

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
              RF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKV  D  SNRIKHI DIF+E GSR
Sbjct: 2943 KHRFCDQFSPMLIDRNDLWSLADSTIIRMPLSSDCLKVGPDLESNRIKHIIDIFMEHGSR 3002

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHPCQNFSISIDPSSSI+RNPF EKKWRKFQLSR+FSSSN
Sbjct: 3003 ALLFLKSVLQVSISTWEEGHSHPCQNFSISIDPSSSILRNPFLEKKWRKFQLSRIFSSSN 3062

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT++DRWL+VLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 3063 AVIKMHVIDVNLYSEGTTVMDRWLVVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 3122

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG  AN+YS SSIM PLPLSG I +PVT+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 3123 NGDHANVYSMSSIMAPLPLSGSIKMPVTILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 3182

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3183 NQLIESWNR 3191



 Score =  115 bits (287), Expect = 6e-24
 Identities = 110/387 (28%), Positives = 168/387 (43%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL ++L ++QGPAL+A +  A  + ++  +   +    
Sbjct: 45   ADDAGATTVSLCLDRRSHGGDSLLSNSLAQWQGPALLA-YNDAVFTEDDFVSISKIGGSS 103

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S + P  K     G
Sbjct: 104  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSASNP-GKRIDFAG 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL--SPNCLKVESDFGSNRIKHISDIFI 562
            ++    +RDQFSP       + S    T+ R PL  +    K +    +   + IS +F+
Sbjct: 163  SSALSFYRDQFSPYCAFGCNMQSPFSGTLFRFPLRNADQAAKSKLSRQAYSPEDISSMFV 222

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + +  W+ G   P + +S S+   SS+      +  W +  L
Sbjct: 223  QLFEEGVLTLLFLKSVLCIEMYLWDVGEPEPKKIYSCSV---SSVT----DDTVWHRQAL 275

Query: 734  SRLFSSSNAAMKMHVIDVTLCS------EGTTIIDRWLLVLSLGSGQTR----NMALDRR 883
             RL    N+  ++    +   S      E     +++ +V ++ S  +R         + 
Sbjct: 276  LRLSKCLNSTSEVDAFPLDFLSERINGDEAERQTEKFYVVQTMASMSSRIGSFATTASKE 335

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
            Y   +L P A IAA IS N   +NI         LPL     L V V G F V  N    
Sbjct: 336  Y-DIHLLPWASIAACISDNSLNSNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 391

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++   WNR
Sbjct: 392  ------RGIWYGDDMDRSGKVRSIWNR 412



 Score = 66.6 bits (161), Expect = 5e-08
 Identities = 81/337 (24%), Positives = 134/337 (39%), Gaps = 24/337 (7%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK  +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1413 AEDAGASEVIFLLDKSHYGTSSILSPEMADWQGPALYCFNDSIFSPQDLYAISRIGQESK 1472

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    +FDP    L  +S + P  ++    G 
Sbjct: 1473 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVLFDPHASNLPGISPSHPGLRI-KFVGR 1531

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSP+L    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1532 QILEQFPDQFSPLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 1590

Query: 554  IFIEQGSRALLFLKSVLEVSISTWE-EGH--------SHPCQNFSISIDPSSSIMRNPFS 706
             F E  S  LLFL +V  +SI   E  GH        S  C          +  + N F 
Sbjct: 1591 TFSEVVSETLLFLHNVKSISIFVKEGTGHEMHLLHRVSRTCIGEPEIGSTEAQDVFNFFK 1650

Query: 707  EKK---WRKFQLSRLFSSS---NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNM 868
            E +     + Q  +  S S   +   K   I +T  S  +     W+    LG G  +  
Sbjct: 1651 ESRHVGMNRVQFLKKISLSIGRDLPYKCQKILITEQSTSSRNSHYWITTECLGDGNAQKR 1710

Query: 869  ALDR-RYLAYNLTPVAGIAALISRNGHPANIYSRSSI 976
              +      YN  P A +AA ++      N+   S +
Sbjct: 1711 TSETVNSNCYNFVPWACVAAYLNSVKVDGNLVESSEV 1747


>gb|KYP37061.1| Sacsin [Cajanus cajan]
          Length = 3973

 Score =  648 bits (1671), Expect = 0.0
 Identities = 315/369 (85%), Positives = 337/369 (91%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAK+LHLIYDKREHPRQSLLQHNLGEFQGPAL+AIFE A LSREE SNFQL PPWR
Sbjct: 2212 ADCCKAKRLHLIYDKREHPRQSLLQHNLGEFQGPALIAIFEGACLSREEFSNFQLRPPWR 2271

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLVCCYSICD+LSV+SGGYFYMFDPRGL L A STN P+AKMFSL GT+L
Sbjct: 2272 LRGNTINYGLGLVCCYSICDILSVISGGYFYMFDPRGLVLGAPSTNGPSAKMFSLIGTDL 2331

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
              RF DQFSPMLID+N+LWSL+DSTIIRMPLS +CLKV  D  SNRIKHI DIF+E GSR
Sbjct: 2332 KHRFCDQFSPMLIDRNDLWSLADSTIIRMPLSSDCLKVGPDLESNRIKHIIDIFMEHGSR 2391

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHSHPCQNFSISIDPSSSI+RNPF EKKWRKFQLSR+FSSSN
Sbjct: 2392 ALLFLKSVLQVSISTWEEGHSHPCQNFSISIDPSSSILRNPFLEKKWRKFQLSRIFSSSN 2451

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT++DRWL+VLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 2452 AVIKMHVIDVNLYSEGTTVMDRWLVVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 2511

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG  AN+YS SSIM PLPLSG I +PVT+LGCFLVCHN GRYLFKYQDRGASA GHFDAG
Sbjct: 2512 NGDHANVYSMSSIMAPLPLSGSIKMPVTILGCFLVCHNRGRYLFKYQDRGASAEGHFDAG 2571

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 2572 NQLIESWNR 2580



 Score =  115 bits (287), Expect = 6e-24
 Identities = 110/387 (28%), Positives = 168/387 (43%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL ++L ++QGPAL+A +  A  + ++  +   +    
Sbjct: 45   ADDAGATTVSLCLDRRSHGGDSLLSNSLAQWQGPALLA-YNDAVFTEDDFVSISKIGGSS 103

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S + P  K     G
Sbjct: 104  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSASNP-GKRIDFAG 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL--SPNCLKVESDFGSNRIKHISDIFI 562
            ++    +RDQFSP       + S    T+ R PL  +    K +    +   + IS +F+
Sbjct: 163  SSALSFYRDQFSPYCAFGCNMQSPFSGTLFRFPLRNADQAAKSKLSRQAYSPEDISSMFV 222

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + +  W+ G   P + +S S+   SS+      +  W +  L
Sbjct: 223  QLFEEGVLTLLFLKSVLCIEMYLWDVGEPEPKKIYSCSV---SSVT----DDTVWHRQAL 275

Query: 734  SRLFSSSNAAMKMHVIDVTLCS------EGTTIIDRWLLVLSLGSGQTR----NMALDRR 883
             RL    N+  ++    +   S      E     +++ +V ++ S  +R         + 
Sbjct: 276  LRLSKCLNSTSEVDAFPLDFLSERINGDEAERQTEKFYVVQTMASMSSRIGSFATTASKE 335

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
            Y   +L P A IAA IS N   +NI         LPL     L V V G F V  N    
Sbjct: 336  Y-DIHLLPWASIAACISDNSLNSNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 391

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++   WNR
Sbjct: 392  ------RGIWYGDDMDRSGKVRSIWNR 412


>dbj|GAU44495.1| hypothetical protein TSUD_13070 [Trifolium subterraneum]
          Length = 4764

 Score =  645 bits (1665), Expect = 0.0
 Identities = 313/369 (84%), Positives = 337/369 (91%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC+AKKLHLIYDKREHPRQSLLQHNLGE+QGP LVAIFE A LSREE SNFQLLPPWR
Sbjct: 2825 ADCCQAKKLHLIYDKREHPRQSLLQHNLGEYQGPGLVAIFEGACLSREEFSNFQLLPPWR 2884

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+N GLGLVCCYSICDLLS+VSGGYFYMFDPRGL LAALSTN P+AKMF L GT+L
Sbjct: 2885 LRGNTLNNGLGLVCCYSICDLLSIVSGGYFYMFDPRGLVLAALSTNAPSAKMFPLIGTDL 2944

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
             QRF DQFSPM +D N+LWS SDSTIIRMPLS + LK+  D G+NRIKHI+DIF+E GSR
Sbjct: 2945 KQRFHDQFSPMFMDHNDLWSSSDSTIIRMPLSSDSLKIGCDLGTNRIKHITDIFMEHGSR 3004

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVLEVSISTWEEG SHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSS+
Sbjct: 3005 ALLFLKSVLEVSISTWEEGQSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSH 3064

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AA+KMHVIDV++CSEGTT +DRWLLVL+LGSGQTRNMALDRRYLAYNLTP+AGIAALISR
Sbjct: 3065 AAIKMHVIDVSVCSEGTTFVDRWLLVLTLGSGQTRNMALDRRYLAYNLTPIAGIAALISR 3124

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH  NIY   SIMTPLPLSG I+LPVTV GCFLVCHN GRYLFKYQD+GASA G++DAG
Sbjct: 3125 NGHHTNIYPTGSIMTPLPLSGHINLPVTVFGCFLVCHNRGRYLFKYQDKGASAAGNYDAG 3184

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3185 NQLIESWNR 3193



 Score =  113 bits (283), Expect = 2e-23
 Identities = 109/387 (28%), Positives = 165/387 (42%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D R H R SLL  +L ++QGPAL+A + +A  S E+  +   +    
Sbjct: 45   ADDAGATTVSLCLDLRSHGRDSLLSDSLSQWQGPALLA-YNNAVFSEEDFVSISKIGGSS 103

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K      
Sbjct: 104  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSAANP-GKRIDFTS 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI--KHISDIFI 562
            ++    ++DQF P      ++ S    T+ R PL        S         + IS +F+
Sbjct: 163  SSALSFYKDQFLPFCAFGCDMQSPFSGTLFRFPLRNADQATRSKLSRQAYSPEDISSMFV 222

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + +  W++G   P +  S S+   SS+ +    +  W +  L
Sbjct: 223  QLFEEGIFTLLFLKSVLCIEMYVWDDGEPEPKKIHSCSV---SSVTQ----DTVWHRQAL 275

Query: 734  SRLFSSSNAAMKMHVIDVTLCSEGTTII------DRWLLVLSLGSGQTR----NMALDRR 883
             RL   SN   +M    +   SE  + +      +R+ +V ++ S  +R         + 
Sbjct: 276  LRLSKCSNTTTEMDAFPLDFVSETVSGLETERQTERFYVVQTMASTSSRIGSFATTASKE 335

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
            Y   +L P A +AA IS +     +         LPL     L V V G F V  N    
Sbjct: 336  Y-DIHLMPWASVAACISDDSPNNKVLRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 391

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++  +WNR
Sbjct: 392  ------RGIWYGDDMDRSGKVRSTWNR 412



 Score = 65.1 bits (157), Expect = 2e-07
 Identities = 55/201 (27%), Positives = 91/201 (45%), Gaps = 8/201 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1414 AEDAGASEVIFLLDKSQYGTSSVLSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1473

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1474 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1532

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFS ML    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1533 QILEQFPDQFSSMLHFGCDLQQSFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 1591

Query: 554  IFIEQGSRALLFLKSVLEVSI 616
             F E  S  LLFL +V  +SI
Sbjct: 1592 AFSEVVSETLLFLHNVKSISI 1612


>ref|XP_019420625.1| PREDICTED: sacsin isoform X1 [Lupinus angustifolius]
          Length = 4759

 Score =  634 bits (1635), Expect = 0.0
 Identities = 316/369 (85%), Positives = 337/369 (91%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAKKLHLIYDKREHP QSLLQHNLGE+QGPALV IFE A LS+EE SNFQL PPWR
Sbjct: 2824 ADCCKAKKLHLIYDKREHPCQSLLQHNLGEYQGPALVVIFEGACLSQEEFSNFQLRPPWR 2883

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRG T+NYGLGLV CYSICDLLSVVSGGYFYMFDPRGL LAA STN P+AKMFSL GT+L
Sbjct: 2884 LRGTTLNYGLGLVSCYSICDLLSVVSGGYFYMFDPRGLVLAAPSTNAPSAKMFSLIGTDL 2943

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFS MLIDQN+LWSLSDSTIIRMPLS +CLKV SD GSNRIKHI+DIF+E GSR
Sbjct: 2944 TQRFSDQFSSMLIDQNDLWSLSDSTIIRMPLSSDCLKVGSDSGSNRIKHITDIFMEHGSR 3003

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWE G  HP +NFSIS+DPSSS+MRNPFSEKKWRKFQLSRLFSSSN
Sbjct: 3004 ALLFLKSVLQVSISTWEVGSPHPLKNFSISVDPSSSVMRNPFSEKKWRKFQLSRLFSSSN 3063

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AAMKMHVIDV+L S GTTIIDRWL+VLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 3064 AAMKMHVIDVSLYSGGTTIIDRWLVVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 3123

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN++S SSIM+PLPLSG +++PVTVLGCFLVCHN GRYLFKYQDR A A GHFDAG
Sbjct: 3124 NGHHANVFSMSSIMSPLPLSGYLNIPVTVLGCFLVCHNRGRYLFKYQDR-ALAEGHFDAG 3182

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3183 NQLIESWNR 3191



 Score =  111 bits (277), Expect = 1e-22
 Identities = 108/386 (27%), Positives = 163/386 (42%), Gaps = 17/386 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A ++ L  D+R H R SLL  +L ++QGPAL+A F  AS + E+  +   +    
Sbjct: 45   ADDAGATRVSLCLDRRSHGRDSLLSDSLSQWQGPALLA-FNDASFTEEDFVSISKIGGSS 103

Query: 218  LRG---NTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
             +G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K      
Sbjct: 104  KQGQSWKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSAANP-GKRIDFTS 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI--KHISDIFI 562
            ++      DQFSP      ++ +    T+ R PL        S         + IS +F+
Sbjct: 163  SSALSYCMDQFSPYCAFGCDMQNPFAGTLFRFPLRNADQAARSKLSRQAYSPEDISSMFV 222

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + I  W+ G   P +  S S+   SS+  +    ++     L
Sbjct: 223  QLFEEGVLTLLFLKSVLCIEIYVWDAGEPEPKKIHSCSV---SSVTDDTVCHRQ----AL 275

Query: 734  SRLFSSSNAAMKMHVIDVTLCSEGTTII------DRWLLVLSLGSGQTR---NMALDRRY 886
             RL    N   +M    +   +E    +      DR+ +V ++    +R     +   + 
Sbjct: 276  LRLSRCLNTTSEMDAFSLDFSTEAVCGVETERKTDRFYVVQTMAPASSRIGSFASTASKE 335

Query: 887  LAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYL 1066
               +L P A +AA IS N    ++         LPL     L V V G F V  N     
Sbjct: 336  YDIHLLPWASVAACISDNSLNNSVLRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR---- 391

Query: 1067 FKYQDRGASAGGHFDAGIQLIESWNR 1144
                 RG   G   D   ++  +WNR
Sbjct: 392  -----RGIWYGDDMDRSGKVRSNWNR 412



 Score = 67.8 bits (164), Expect = 2e-08
 Identities = 81/323 (25%), Positives = 132/323 (40%), Gaps = 24/323 (7%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1412 AEDAGASEVRFLLDKSQYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1471

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1472 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHACNLPGISPSHPGLRI-KFVGR 1530

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFS +L    ++      T+ R PL      S + +K E  +    +  +  
Sbjct: 1531 QILEQFPDQFSSLLHFGCDMQHSFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVGSLFA 1589

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH-SHPCQNFSISIDPSSSI----MRNPFS 706
             F E  S  LLFL++V  +SI       +E H  H     SI    + S     M N F 
Sbjct: 1590 AFSEVVSETLLFLRNVKSISIYVKEGAGQEMHLLHHVHRTSIDEPETGSTEAQEMFNVFK 1649

Query: 707  EKK---WRKFQLSRLFSSS---NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNM 868
            EK+     + Q  +  S S   N   K   I +T  S        W+    LG G   N 
Sbjct: 1650 EKRHVGMNRSQFLKKLSLSIDRNLPYKCQKILITEKSTSGHNSHFWITTECLGGGNASNG 1709

Query: 869  ALD-RRYLAYNLTPVAGIAALIS 934
              +  +   +N  P A +A  ++
Sbjct: 1710 TSEASKVNCHNFVPWACVATRLN 1732


>ref|XP_019420628.1| PREDICTED: sacsin isoform X2 [Lupinus angustifolius]
          Length = 4757

 Score =  634 bits (1635), Expect = 0.0
 Identities = 316/369 (85%), Positives = 337/369 (91%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCCKAKKLHLIYDKREHP QSLLQHNLGE+QGPALV IFE A LS+EE SNFQL PPWR
Sbjct: 2824 ADCCKAKKLHLIYDKREHPCQSLLQHNLGEYQGPALVVIFEGACLSQEEFSNFQLRPPWR 2883

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRG T+NYGLGLV CYSICDLLSVVSGGYFYMFDPRGL LAA STN P+AKMFSL GT+L
Sbjct: 2884 LRGTTLNYGLGLVSCYSICDLLSVVSGGYFYMFDPRGLVLAAPSTNAPSAKMFSLIGTDL 2943

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFS MLIDQN+LWSLSDSTIIRMPLS +CLKV SD GSNRIKHI+DIF+E GSR
Sbjct: 2944 TQRFSDQFSSMLIDQNDLWSLSDSTIIRMPLSSDCLKVGSDSGSNRIKHITDIFMEHGSR 3003

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWE G  HP +NFSIS+DPSSS+MRNPFSEKKWRKFQLSRLFSSSN
Sbjct: 3004 ALLFLKSVLQVSISTWEVGSPHPLKNFSISVDPSSSVMRNPFSEKKWRKFQLSRLFSSSN 3063

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AAMKMHVIDV+L S GTTIIDRWL+VLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 3064 AAMKMHVIDVSLYSGGTTIIDRWLVVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 3123

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN++S SSIM+PLPLSG +++PVTVLGCFLVCHN GRYLFKYQDR A A GHFDAG
Sbjct: 3124 NGHHANVFSMSSIMSPLPLSGYLNIPVTVLGCFLVCHNRGRYLFKYQDR-ALAEGHFDAG 3182

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3183 NQLIESWNR 3191



 Score =  111 bits (277), Expect = 1e-22
 Identities = 108/386 (27%), Positives = 163/386 (42%), Gaps = 17/386 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A ++ L  D+R H R SLL  +L ++QGPAL+A F  AS + E+  +   +    
Sbjct: 45   ADDAGATRVSLCLDRRSHGRDSLLSDSLSQWQGPALLA-FNDASFTEEDFVSISKIGGSS 103

Query: 218  LRG---NTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
             +G    T  +G+G    Y + DL S VSG Y  +FDP+G+ L  +S   P  K      
Sbjct: 104  KQGQSWKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGVYLPRVSAANP-GKRIDFTS 162

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI--KHISDIFI 562
            ++      DQFSP      ++ +    T+ R PL        S         + IS +F+
Sbjct: 163  SSALSYCMDQFSPYCAFGCDMQNPFAGTLFRFPLRNADQAARSKLSRQAYSPEDISSMFV 222

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G   LLFLKSVL + I  W+ G   P +  S S+   SS+  +    ++     L
Sbjct: 223  QLFEEGVLTLLFLKSVLCIEIYVWDAGEPEPKKIHSCSV---SSVTDDTVCHRQ----AL 275

Query: 734  SRLFSSSNAAMKMHVIDVTLCSEGTTII------DRWLLVLSLGSGQTR---NMALDRRY 886
             RL    N   +M    +   +E    +      DR+ +V ++    +R     +   + 
Sbjct: 276  LRLSRCLNTTSEMDAFSLDFSTEAVCGVETERKTDRFYVVQTMAPASSRIGSFASTASKE 335

Query: 887  LAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYL 1066
               +L P A +AA IS N    ++         LPL     L V V G F V  N     
Sbjct: 336  YDIHLLPWASVAACISDNSLNNSVLRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR---- 391

Query: 1067 FKYQDRGASAGGHFDAGIQLIESWNR 1144
                 RG   G   D   ++  +WNR
Sbjct: 392  -----RGIWYGDDMDRSGKVRSNWNR 412



 Score = 67.8 bits (164), Expect = 2e-08
 Identities = 81/323 (25%), Positives = 132/323 (40%), Gaps = 24/323 (7%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1412 AEDAGASEVRFLLDKSQYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1471

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1472 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHACNLPGISPSHPGLRI-KFVGR 1530

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFS +L    ++      T+ R PL      S + +K E  +    +  +  
Sbjct: 1531 QILEQFPDQFSSLLHFGCDMQHSFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVGSLFA 1589

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH-SHPCQNFSISIDPSSSI----MRNPFS 706
             F E  S  LLFL++V  +SI       +E H  H     SI    + S     M N F 
Sbjct: 1590 AFSEVVSETLLFLRNVKSISIYVKEGAGQEMHLLHHVHRTSIDEPETGSTEAQEMFNVFK 1649

Query: 707  EKK---WRKFQLSRLFSSS---NAAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNM 868
            EK+     + Q  +  S S   N   K   I +T  S        W+    LG G   N 
Sbjct: 1650 EKRHVGMNRSQFLKKLSLSIDRNLPYKCQKILITEKSTSGHNSHFWITTECLGGGNASNG 1709

Query: 869  ALD-RRYLAYNLTPVAGIAALIS 934
              +  +   +N  P A +A  ++
Sbjct: 1710 TSEASKVNCHNFVPWACVATRLN 1732


>ref|XP_007153087.1| hypothetical protein PHAVU_003G005800g [Phaseolus vulgaris]
 gb|ESW25081.1| hypothetical protein PHAVU_003G005800g [Phaseolus vulgaris]
          Length = 4756

 Score =  624 bits (1609), Expect = 0.0
 Identities = 302/369 (81%), Positives = 332/369 (89%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC+AK+LHLIYDKREHPRQSLLQHNLGEFQGPALVAIFE A LSREE SNFQL PPWR
Sbjct: 2819 ADCCQAKRLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFEGACLSREEFSNFQLRPPWR 2878

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLV CYSICDLLSV+S G+FYMFDP GL L    TN P+AKMFSL G +L
Sbjct: 2879 LRGNTINYGLGLVSCYSICDLLSVISSGFFYMFDPHGLVLGTPLTNAPSAKMFSLIGNDL 2938

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPML+D+N+LWSL+DSTIIRMPLS +CLK   D GSNRI+ I+DIF++ GSR
Sbjct: 2939 TQRFCDQFSPMLVDRNDLWSLADSTIIRMPLSSDCLKDGPDLGSNRIRLITDIFMKHGSR 2998

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
             LLFLKSVL+VSISTWEEG+ +P QNFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 2999 TLLFLKSVLQVSISTWEEGNPNPSQNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 3058

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+ LSLGSGQTRNMALDRRYLAYNLTPVAGIAAL+S 
Sbjct: 3059 AMIKMHVIDVDLYSEGTTVIDRWLVALSLGSGQTRNMALDRRYLAYNLTPVAGIAALVSS 3118

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NGH AN+YSRSSIM PLPLSGCI++PVTV+GCFLVCHN GR+LFKYQDRGAS  GHFDAG
Sbjct: 3119 NGHHANVYSRSSIMAPLPLSGCINMPVTVIGCFLVCHNRGRFLFKYQDRGASTEGHFDAG 3178

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3179 NQLIESWNR 3187



 Score =  118 bits (296), Expect = 4e-25
 Identities = 111/386 (28%), Positives = 163/386 (42%), Gaps = 17/386 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL ++L ++QGPAL+A +  A+ + ++  +   +    
Sbjct: 43   ADDAGATTVSLCLDRRSHRGDSLLANSLAQWQGPALLA-YNDAAFTEDDFVSISKIGGSA 101

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G  L  +S   P  K     G
Sbjct: 102  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGAYLPRVSAANP-GKRIDFTG 160

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI--KHISDIFI 562
            ++    +RDQFSP      ++ S    T+ R PL        S         + IS +F+
Sbjct: 161  SSALSFYRDQFSPYCAFGCDMQSPFSGTLFRFPLRNADQAARSKLSRQAYSPEDISSMFV 220

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G  ALLFLKSVL + +  W+ G   P + +S S+   S        +  W +  L
Sbjct: 221  QLFEEGVLALLFLKSVLCIEMYLWDVGEPKPKKIYSCSVSSVS-------DDTVWHRQAL 273

Query: 734  SRLFSSSNAAMKMHVIDVTLCSE---GTTI---IDRWLLVLSLGSGQTR---NMALDRRY 886
             RL    N   +M    +   SE   G  +    +R+ +V ++ +  +R         + 
Sbjct: 274  VRLSKCLNTTAEMDAFQLEFLSERISGDEVKRQTERFYVVQTMAAASSRIGSFATTASKD 333

Query: 887  LAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYL 1066
               +L P A IAA IS N    NI         LPL     L V V G F V  N     
Sbjct: 334  YDIHLLPWASIAACISENSTKNNILRTGQAFCFLPLPVRTGLTVQVNGFFEVSSNR---- 389

Query: 1067 FKYQDRGASAGGHFDAGIQLIESWNR 1144
                 RG   G   D   ++   WNR
Sbjct: 390  -----RGIWYGDDMDRSGKVRSIWNR 410



 Score = 65.9 bits (159), Expect = 9e-08
 Identities = 55/201 (27%), Positives = 91/201 (45%), Gaps = 8/201 (3%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + D   +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1411 AEDAGASEVIFLLDNSHYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1470

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1471 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1529

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSPML    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1530 QILEQFPDQFSPMLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEI-YTPEDVRSLFA 1588

Query: 554  IFIEQGSRALLFLKSVLEVSI 616
             F E  S  LLFL++V  +SI
Sbjct: 1589 SFSEVVSETLLFLRNVKSISI 1609


>ref|XP_017426833.1| PREDICTED: sacsin isoform X1 [Vigna angularis]
 dbj|BAT98743.1| hypothetical protein VIGAN_10007200 [Vigna angularis var. angularis]
          Length = 4756

 Score =  620 bits (1599), Expect = 0.0
 Identities = 302/369 (81%), Positives = 331/369 (89%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC+AK+LHL+YDKREHPRQ LLQHNLGEFQGPALV IFE A LSREE SN QL PPWR
Sbjct: 2821 ADCCQAKRLHLVYDKREHPRQFLLQHNLGEFQGPALVVIFEGACLSREEFSNLQLRPPWR 2880

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLV CYSICDLLSV+SGG+FYMFDPRGL L     N P+AKMFSL G +L
Sbjct: 2881 LRGNTINYGLGLVSCYSICDLLSVMSGGFFYMFDPRGLVLGTPLANAPSAKMFSLIGNDL 2940

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLI+Q +LWSL+DSTIIRMPLS +CLK   D GSNRI+HI DIF++ GSR
Sbjct: 2941 TQRFCDQFSPMLIEQTDLWSLADSTIIRMPLSFDCLKDGPDLGSNRIRHIIDIFMKHGSR 3000

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHS+P +NFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 3001 ALLFLKSVLQVSISTWEEGHSNPRRNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 3060

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+ LSLGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 3061 AMIKMHVIDVNLYSEGTTVIDRWLVALSLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 3120

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG+ AN +SRSSIM PLPLSGCI+LPVT++GCFLVCHN+GRYLFKYQDRGAS  GHFDAG
Sbjct: 3121 NGYHANDFSRSSIMAPLPLSGCINLPVTIIGCFLVCHNTGRYLFKYQDRGASTQGHFDAG 3180

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3181 NQLIESWNR 3189



 Score =  120 bits (300), Expect = 1e-25
 Identities = 113/387 (29%), Positives = 165/387 (42%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL ++L ++QGPAL+A + +A  + ++  +   +    
Sbjct: 42   ADDAGATTVSLCLDRRTHRGDSLLTNSLAQWQGPALLA-YNNAVFTEDDFVSISKIGGSA 100

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G  L  +S   P  K     G
Sbjct: 101  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGAYLPRVSAANP-GKRIDFTG 159

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI--KHISDIFI 562
            ++    +RDQFSP      ++ S    T+ R PL      V S         + IS +F+
Sbjct: 160  SSALSFYRDQFSPYCAFGCDMQSPFSGTLFRFPLRNADQAVRSKLSRQAYSPEDISSMFV 219

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G  ALLFLKSVL + +  W+ G S P +  S S+   S        +  W +  L
Sbjct: 220  QLFEEGVLALLFLKSVLCIEMYLWDVGESEPKKIHSCSVSSVS-------DDTVWHRQAL 272

Query: 734  SRLFSSSNAAMKMHVIDVTLCS------EGTTIIDRWLLVLSLGSGQTR----NMALDRR 883
             RL    N   +M    +   S      E     +R+ +V ++ +  +R    +    + 
Sbjct: 273  VRLSKHLNTTAEMDAFQLDFVSERISGDEAKRQTERFYVVQTMAAASSRIGSFSTTASKD 332

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
            Y   +L P A IAA IS N    NI         LPL     L V V G F V  N    
Sbjct: 333  Y-DIHLLPWASIAACISENLSNNNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 388

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++  +WNR
Sbjct: 389  ------RGIWYGDDMDRSGKVRSTWNR 409



 Score = 71.2 bits (173), Expect = 2e-09
 Identities = 81/325 (24%), Positives = 139/325 (42%), Gaps = 26/325 (8%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + D   +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1410 AEDAGASEVIFLLDNSHYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1469

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1470 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1528

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSPML    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1529 KILEQFPDQFSPMLHFGCDLQHPFPGTLFRFPLRSAGVASRSQIKKEI-YTPEDVRSLFT 1587

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH--SHPCQNFSISIDPSSSIMRNPFS-EK 712
             F E  S  LLFL++V  +SI     T  E H     C+      +  S+  ++ F+  K
Sbjct: 1588 SFSEVVSETLLFLRNVKSISIFVKEGTMNEMHLLHRVCRTCIGEPETGSAEAQDVFNFFK 1647

Query: 713  KWRKFQLSRLFSSSNAAM--------KMHVIDVTLCSEGTTIIDRWLLVLSLGSG---QT 859
            + R+  ++R+    N ++        K   I +T  S  +     W+    LG G   + 
Sbjct: 1648 ESRRVGMNRVQFLKNLSLSIGRDLPYKCQKIIITEQSTSSRTSHYWITTECLGDGNAQKK 1707

Query: 860  RNMALDRRYLAYNLTPVAGIAALIS 934
            R+   +  Y  YN  P A +AA ++
Sbjct: 1708 RSETANSSY--YNFVPWACVAAYLN 1730


>ref|XP_017426834.1| PREDICTED: sacsin isoform X2 [Vigna angularis]
          Length = 4754

 Score =  620 bits (1599), Expect = 0.0
 Identities = 302/369 (81%), Positives = 331/369 (89%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC+AK+LHL+YDKREHPRQ LLQHNLGEFQGPALV IFE A LSREE SN QL PPWR
Sbjct: 2821 ADCCQAKRLHLVYDKREHPRQFLLQHNLGEFQGPALVVIFEGACLSREEFSNLQLRPPWR 2880

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLV CYSICDLLSV+SGG+FYMFDPRGL L     N P+AKMFSL G +L
Sbjct: 2881 LRGNTINYGLGLVSCYSICDLLSVMSGGFFYMFDPRGLVLGTPLANAPSAKMFSLIGNDL 2940

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLI+Q +LWSL+DSTIIRMPLS +CLK   D GSNRI+HI DIF++ GSR
Sbjct: 2941 TQRFCDQFSPMLIEQTDLWSLADSTIIRMPLSFDCLKDGPDLGSNRIRHIIDIFMKHGSR 3000

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHS+P +NFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 3001 ALLFLKSVLQVSISTWEEGHSNPRRNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 3060

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+ LSLGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 3061 AMIKMHVIDVNLYSEGTTVIDRWLVALSLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 3120

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG+ AN +SRSSIM PLPLSGCI+LPVT++GCFLVCHN+GRYLFKYQDRGAS  GHFDAG
Sbjct: 3121 NGYHANDFSRSSIMAPLPLSGCINLPVTIIGCFLVCHNTGRYLFKYQDRGASTQGHFDAG 3180

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 3181 NQLIESWNR 3189



 Score =  120 bits (300), Expect = 1e-25
 Identities = 113/387 (29%), Positives = 165/387 (42%), Gaps = 18/387 (4%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            AD   A  + L  D+R H   SLL ++L ++QGPAL+A + +A  + ++  +   +    
Sbjct: 42   ADDAGATTVSLCLDRRTHRGDSLLTNSLAQWQGPALLA-YNNAVFTEDDFVSISKIGGSA 100

Query: 218  LRGN---TVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRG 388
              G    T  +G+G    Y + DL S VSG Y  +FDP+G  L  +S   P  K     G
Sbjct: 101  KHGQASKTGRFGVGFNSVYHLTDLPSFVSGKYVVLFDPQGAYLPRVSAANP-GKRIDFTG 159

Query: 389  TNLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRI--KHISDIFI 562
            ++    +RDQFSP      ++ S    T+ R PL      V S         + IS +F+
Sbjct: 160  SSALSFYRDQFSPYCAFGCDMQSPFSGTLFRFPLRNADQAVRSKLSRQAYSPEDISSMFV 219

Query: 563  ---EQGSRALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQL 733
               E+G  ALLFLKSVL + +  W+ G S P +  S S+   S        +  W +  L
Sbjct: 220  QLFEEGVLALLFLKSVLCIEMYLWDVGESEPKKIHSCSVSSVS-------DDTVWHRQAL 272

Query: 734  SRLFSSSNAAMKMHVIDVTLCS------EGTTIIDRWLLVLSLGSGQTR----NMALDRR 883
             RL    N   +M    +   S      E     +R+ +V ++ +  +R    +    + 
Sbjct: 273  VRLSKHLNTTAEMDAFQLDFVSERISGDEAKRQTERFYVVQTMAAASSRIGSFSTTASKD 332

Query: 884  YLAYNLTPVAGIAALISRNGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRY 1063
            Y   +L P A IAA IS N    NI         LPL     L V V G F V  N    
Sbjct: 333  Y-DIHLLPWASIAACISENLSNNNILRTGQAFCFLPLPVRTGLSVQVNGFFEVSSNR--- 388

Query: 1064 LFKYQDRGASAGGHFDAGIQLIESWNR 1144
                  RG   G   D   ++  +WNR
Sbjct: 389  ------RGIWYGDDMDRSGKVRSTWNR 409



 Score = 71.2 bits (173), Expect = 2e-09
 Identities = 81/325 (24%), Positives = 139/325 (42%), Gaps = 26/325 (8%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + D   +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1410 AEDAGASEVIFLLDNSHYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1469

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1470 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1528

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSPML    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1529 KILEQFPDQFSPMLHFGCDLQHPFPGTLFRFPLRSAGVASRSQIKKEI-YTPEDVRSLFT 1587

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH--SHPCQNFSISIDPSSSIMRNPFS-EK 712
             F E  S  LLFL++V  +SI     T  E H     C+      +  S+  ++ F+  K
Sbjct: 1588 SFSEVVSETLLFLRNVKSISIFVKEGTMNEMHLLHRVCRTCIGEPETGSAEAQDVFNFFK 1647

Query: 713  KWRKFQLSRLFSSSNAAM--------KMHVIDVTLCSEGTTIIDRWLLVLSLGSG---QT 859
            + R+  ++R+    N ++        K   I +T  S  +     W+    LG G   + 
Sbjct: 1648 ESRRVGMNRVQFLKNLSLSIGRDLPYKCQKIIITEQSTSSRTSHYWITTECLGDGNAQKK 1707

Query: 860  RNMALDRRYLAYNLTPVAGIAALIS 934
            R+   +  Y  YN  P A +AA ++
Sbjct: 1708 RSETANSSY--YNFVPWACVAAYLN 1730


>ref|XP_017426835.1| PREDICTED: sacsin isoform X3 [Vigna angularis]
          Length = 4408

 Score =  620 bits (1599), Expect = 0.0
 Identities = 302/369 (81%), Positives = 331/369 (89%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC+AK+LHL+YDKREHPRQ LLQHNLGEFQGPALV IFE A LSREE SN QL PPWR
Sbjct: 2473 ADCCQAKRLHLVYDKREHPRQFLLQHNLGEFQGPALVVIFEGACLSREEFSNLQLRPPWR 2532

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            LRGNT+NYGLGLV CYSICDLLSV+SGG+FYMFDPRGL L     N P+AKMFSL G +L
Sbjct: 2533 LRGNTINYGLGLVSCYSICDLLSVMSGGFFYMFDPRGLVLGTPLANAPSAKMFSLIGNDL 2592

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
            TQRF DQFSPMLI+Q +LWSL+DSTIIRMPLS +CLK   D GSNRI+HI DIF++ GSR
Sbjct: 2593 TQRFCDQFSPMLIEQTDLWSLADSTIIRMPLSFDCLKDGPDLGSNRIRHIIDIFMKHGSR 2652

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEGHS+P +NFSISIDPSSSI+RNPFSEKKWRKFQLSR+FSSSN
Sbjct: 2653 ALLFLKSVLQVSISTWEEGHSNPRRNFSISIDPSSSILRNPFSEKKWRKFQLSRIFSSSN 2712

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            A +KMHVIDV L SEGTT+IDRWL+ LSLGSGQTRNMALDRRYLAYNLTPVAGIAALIS 
Sbjct: 2713 AMIKMHVIDVNLYSEGTTVIDRWLVALSLGSGQTRNMALDRRYLAYNLTPVAGIAALISS 2772

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG+ AN +SRSSIM PLPLSGCI+LPVT++GCFLVCHN+GRYLFKYQDRGAS  GHFDAG
Sbjct: 2773 NGYHANDFSRSSIMAPLPLSGCINLPVTIIGCFLVCHNTGRYLFKYQDRGASTQGHFDAG 2832

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 2833 NQLIESWNR 2841



 Score = 71.2 bits (173), Expect = 2e-09
 Identities = 81/325 (24%), Positives = 139/325 (42%), Gaps = 26/325 (8%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            A+   A ++  + D   +   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 1062 AEDAGASEVIFLLDNSHYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 1121

Query: 218  LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
            L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 1122 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHASNLPGISPSHPGLRI-KFVGR 1180

Query: 392  NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
             + ++F DQFSPML    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 1181 KILEQFPDQFSPMLHFGCDLQHPFPGTLFRFPLRSAGVASRSQIKKEI-YTPEDVRSLFT 1239

Query: 554  IFIEQGSRALLFLKSVLEVSI----STWEEGH--SHPCQNFSISIDPSSSIMRNPFS-EK 712
             F E  S  LLFL++V  +SI     T  E H     C+      +  S+  ++ F+  K
Sbjct: 1240 SFSEVVSETLLFLRNVKSISIFVKEGTMNEMHLLHRVCRTCIGEPETGSAEAQDVFNFFK 1299

Query: 713  KWRKFQLSRLFSSSNAAM--------KMHVIDVTLCSEGTTIIDRWLLVLSLGSG---QT 859
            + R+  ++R+    N ++        K   I +T  S  +     W+    LG G   + 
Sbjct: 1300 ESRRVGMNRVQFLKNLSLSIGRDLPYKCQKIIITEQSTSSRTSHYWITTECLGDGNAQKK 1359

Query: 860  RNMALDRRYLAYNLTPVAGIAALIS 934
            R+   +  Y  YN  P A +AA ++
Sbjct: 1360 RSETANSSY--YNFVPWACVAAYLN 1382


>ref|XP_020989804.1| sacsin [Arachis duranensis]
          Length = 2561

 Score =  615 bits (1585), Expect = 0.0
 Identities = 305/369 (82%), Positives = 333/369 (90%)
 Frame = +2

Query: 38   ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
            ADCC AK+LHLIYDKREHPRQSLLQHNLGE+QGPAL+AIFE A LSREE SNFQL PPWR
Sbjct: 1427 ADCCNAKRLHLIYDKREHPRQSLLQHNLGEYQGPALIAIFEGACLSREEFSNFQLHPPWR 1486

Query: 218  LRGNTVNYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGTNL 397
            +RGNT+NYGLGLVCCYSICDLLSV+SGGYFYMFDP G+ LAA STN P+AKMFSL G  L
Sbjct: 1487 IRGNTLNYGLGLVCCYSICDLLSVISGGYFYMFDPHGMVLAAPSTNAPSAKMFSLIGMLL 1546

Query: 398  TQRFRDQFSPMLIDQNELWSLSDSTIIRMPLSPNCLKVESDFGSNRIKHISDIFIEQGSR 577
              RF DQFSPMLIDQ++LWSLSDSTIIRMPLS N LKV  D GS++IKHI++IF+E GSR
Sbjct: 1547 --RFCDQFSPMLIDQHDLWSLSDSTIIRMPLSSNHLKVGPDHGSDKIKHITEIFMEYGSR 1604

Query: 578  ALLFLKSVLEVSISTWEEGHSHPCQNFSISIDPSSSIMRNPFSEKKWRKFQLSRLFSSSN 757
            ALLFLKSVL+VSISTWEEG S P QNFS+SIDPSSS+MRNPFSEKKWRKFQLSRLF SSN
Sbjct: 1605 ALLFLKSVLQVSISTWEEGSSQPLQNFSVSIDPSSSVMRNPFSEKKWRKFQLSRLFGSSN 1664

Query: 758  AAMKMHVIDVTLCSEGTTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 937
            AA+KMHVIDV+L SEG TIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR
Sbjct: 1665 AAIKMHVIDVSLYSEGVTIIDRWLLVLSLGSGQTRNMALDRRYLAYNLTPVAGIAALISR 1724

Query: 938  NGHPANIYSRSSIMTPLPLSGCIDLPVTVLGCFLVCHNSGRYLFKYQDRGASAGGHFDAG 1117
            NG  AN+YS SSIM+PLPLSGC+++PVTV+GCFLV H+ GRYLFKYQDR ASA G +DAG
Sbjct: 1725 NGQHANVYSVSSIMSPLPLSGCMNMPVTVVGCFLVRHSRGRYLFKYQDRRASAEGRYDAG 1784

Query: 1118 IQLIESWNR 1144
             QLIESWNR
Sbjct: 1785 NQLIESWNR 1793



 Score = 66.6 bits (161), Expect = 5e-08
 Identities = 57/210 (27%), Positives = 96/210 (45%), Gaps = 8/210 (3%)
 Frame = +2

Query: 38  ADCCKAKKLHLIYDKREHPRQSLLQHNLGEFQGPALVAIFESASLSREELSNFQLLPPWR 217
           A+   A ++  + DK ++   S+L   + ++QGPAL    +S    ++  +  ++    +
Sbjct: 52  AEDAGASEVIFLLDKSQYGTSSILSPEMADWQGPALYCFNDSVFSPQDLYAISRIGQESK 111

Query: 218 LRGNTV--NYGLGLVCCYSICDLLSVVSGGYFYMFDPRGLALAALSTNTPTAKMFSLRGT 391
           L        +GLG  C Y   D+   VSG    MFDP    L  +S + P  ++    G 
Sbjct: 112 LEKAFAIGRFGLGFNCVYHFTDIPMFVSGENIVMFDPHACNLPGISPSHPGLRI-KFVGR 170

Query: 392 NLTQRFRDQFSPMLIDQNELWSLSDSTIIRMPL------SPNCLKVESDFGSNRIKHISD 553
            + ++F DQFS +L    +L      T+ R PL      S + +K E  +    ++ +  
Sbjct: 171 QILEQFPDQFSSLLHFGCDLQHPFPGTLFRFPLRTAGVASRSQIKKEV-YTPEDVRSLFA 229

Query: 554 IFIEQGSRALLFLKSVLEVSISTWEEGHSH 643
            F E  S  LLFL++V  +SI   +EG  H
Sbjct: 230 AFSEVVSETLLFLRNVKSISIFV-KEGTGH 258